Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| comparative genomics of canine-isolated leishmania (leishmania) amazonensis from an endemic focus of visceral leishmaniasis in governador valadares, southeastern brazil. | leishmaniasis is a highly diverse group of diseases caused by kinetoplastid of the genus leishmania. these parasites are taxonomically diverse, with human pathogenic species separated into two subgenera according to their development site inside the alimentary tract of the sand fly insect vector. the disease encompasses a variable spectrum of clinical manifestations with tegumentary or visceral symptoms. among the causative species in brazil, leishmania (leishmania) amazonensis is an important e ... | 2017 | 28091623 |
| leishmaniasis recidivans in ethiopia: cutaneous and mucocutaneous features. | cutaneous leishmaniasis (cl) is endemic in ethiopia. an unusual clinical form of this disease is leishmaniasis recidivans (lr), a prolonged, relapsing form of cutaneous leishmaniasis resembling tuberculosis of the skin that may persist for many years with a chronic and relapsing course. this rare variant has been shown to be caused by leishmania tropica species in the old world and by leishmania braziliensis, leishmania amazonensis, leishmania panamensis, and leishmania guyanensis in the new wor ... | 2017 | 28141598 |
| the anti-trichomonas vaginalis phloroglucinol derivative isoaustrobrasilol b modulates extracellular nucleotides hydrolysis. | trichomonas vaginalis causes trichomoniasis, a neglected sexually transmitted disease. due to severe health consequences and treatment failure, new therapeutic alternatives are crucial. phloroglucinols from southern brazilian hypericum species demonstrated anti-t. vaginalis and anti-leishmania amazonensis activities. the modulation of biochemical pathways involved in the control of inflammatory response by ectonucleotidases, ntpdase and ecto-5'-nucleotidase, represents new targets for combating ... | 2017 | 28390095 |
| glycosylated metal chelators as anti-parasitic agents with tunable selectivity. | trypanosoma cruzi and leishmania amazonensis are the causative agents of chagas' disease and leishmaniasis, respectively. these conditions affect millions of people worldwide, especially in developing countries. as such, there is an urgent need for novel, efficient and cost-effective treatments for these diseases, given the growing resistance and side-effects of current therapies. this work details the synthesis and evaluation of the anti-parasitic activity of novel amino- and iminopyridyl metal ... | 2017 | 28382355 |
| exploring the association of surface plasmon resonance with recombinant mhc:ig hybrid protein as a tool for detecting t lymphocytes in mice infected with leishmania (leishmania) amazonensis. | a surface plasmon resonance- (spr-) based recognition method applying h-2 l(d):ig/peptides complexes for ex vivo monitoring cellular immune responses during murine infection with leishmania (leishmania) amazonensis is described. lymphocytes from lesion-draining popliteal lymph nodes were captured on a carboxylated sensor chip surface previously functionalized with h-2 l(d):ig (dimerx) protein bound to synthetic peptides derived from the cooh-terminal region of cysteine proteinase b of l. (l.) am ... | 2017 | 28373990 |
| serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in indigenous reserves in the brazilian amazon region. | leishmaniasis is endemic to the northern, northeastern, central-western, and southeastern regions of brazil. we aimed to assess the epidemiological situation of leishmaniasis in humans and dogs in indigenous villages located in the states of mato grosso and tocantins using a serological survey conducted in may 2011. | 2017 | 28327803 |
| antileishmanial activity of handroanthus serratifolius (vahl) s. grose (bignoniaceae). | this study aimed to evaluate the leishmanicidal activity of ethanol extract, fractions, and isolated substance from handroanthus serratifolius against leishmania amazonensis. furthermore, this activity was related to cytotoxicity, and the selectivity index was determined. the ethanol extract was obtained by maceration of the stem powder, and the extract was subjected to fractionation on chromatographic column. the lapachol was obtained by acid base extraction followed by purification in chromato ... | 2017 | 28286535 |
| a chimera containing cd4+ and cd8+ t-cell epitopes of the leishmania donovani nucleoside hydrolase (nh36) optimizes cross-protection against leishmania amazonesis infection. | the leishmania donovani nucleoside hydrolase (nh36) and nh a34480 of leishmania amazonensis share 93% of sequence identity. in mice, the nh36 induced protection against visceral leishmaniasis is mediated by a cd4+ t cell response against its c-terminal domain (f3). besides this cd4+ th1 response, prevention and cure of l. amazonensis infection require also additional cd8+ and regulatory t-cell responses to the nh36 n-terminal (f1 domain). we investigated if mice vaccination with f1 and f3 domain ... | 2017 | 28280494 |
| leishmania (leishmania) amazonensis induces macrophage mir-294 and mir-721 expression and modulates infection by targeting nos2 and l-arginine metabolism. | leishmania (leishmania) amazonensis is an intracellular protozoan parasite responsible for the cutaneous leishmaniasis. the parasite replicates inside mammalian macrophage to establish infection. host-pathogen interactions result in microrna-mediated post-transcriptional regulation of host genes involved in inflammatory immune response. we analyzed macrophage mirna profiles during l. (l.) amazonensis infection. the regulation of macrophage mirna expression by the parasite correlates with/depends ... | 2017 | 28276497 |
| topical tamoxifen in the therapy of cutaneous leishmaniasis. | the aims of the present work were to test the effect of tamoxifen administered topically and the therapeutic efficacy of tamoxifen and pentavalent antimonial combinations in an experimental model of cutaneous leishmaniasis. balb/c mice infected with a luciferase expressing line of leishmania amazonensis were treated with topical tamoxifen in two different formulations (ethanol or oil-free cream) as monotherapy or in co-administration with pentavalent antimonial. treatment efficacy was evaluated ... | 2017 | 28274283 |
| induction of early autophagic process on leishmania amazonensis by synergistic effect of miltefosine and innovative semi-synthetic thiosemicarbazone. | drug combination therapy is a current trend to treat complex diseases. many benefits are expected from this strategy, such as cytotoxicity decrease, retardation of resistant strains development, and activity increment. this study evaluated in vitro combination between an innovative thiosemicarbazone molecule - bzts with miltefosine, a drug already consolidated in the leishmaniasis treatment, against leishmania amazonensis. cytotoxicity effects were also evaluated on macrophages and erythrocytes. ... | 2017 | 28270805 |
| cutaneous leishmaniasis in french guiana: revising epidemiology with pcr-rflp. | the development of polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) technique for species identification among patients presenting leishmaniasis allowed to better determine the main circulating species in french guiana. | 2017 | 28265182 |
| chemical composition, antiprotozoal and cytotoxic activities of indole alkaloids and benzofuran neolignan of aristolochia cordigera. | this is a comparative study on the intraspecific chemical variability of aristolochia cordigera species, collected in two different regions of brazil, biome cerrado (semiarid) and biome amazônia (coastal). the use of gc-ms and statistical methods led to the identification of 56 compounds. a higher percentage of palmitone and germacrene-d in the hexanes extracts of the leaves of plants from these respective biomes was observed. phytochemical studies on the extracts led to the isolation and identi ... | 2017 | 28264205 |
| differences in the detection of brdu/edu incorporation assays alter the calculation for g1, s, and g2 phases of the cell cycle in trypanosomatids. | trypanosomatids are the etiologic agents of various infectious diseases in humans. they diverged early during eukaryotic evolution and have attracted attention as peculiar models for evolutionary and comparative studies. here, we show a meticulous study comparing the incorporation and detection of the thymidine analogs brdu and edu in leishmania amazonensis, trypanosoma brucei, and trypanosoma cruzi to monitor their dna replication. we used brdu- and edu-incorporated parasites with the respectiv ... | 2017 | 28258618 |
| metacaspase-binding peptide inhibits heat shock-induced death in leishmania (l.) amazonensis. | leishmania (leishmania) amazonensis is an important agent of cutaneous leishmaniasis in brazil. this parasite faces cell death in some situations during transmission to the vertebrate host, and this process seems to be dependent on the activity of metacaspase (mca), an enzyme bearing trypsin-like activity present in protozoans, plants and fungi. in fact, the association between mca expression and cell death induced by different stimuli has been demonstrated for several leishmania species. regula ... | 2017 | 28252649 |
| cytokines and microbicidal molecules regulated by il-32 in thp-1-derived human macrophages infected with new world leishmania species. | interleukin-32 (il-32) is expressed in lesions of patients with american tegumentary leishmaniasis (atl), but its precise role in the disease remains unknown. | 2017 | 28241012 |
| anti-leishmania activity of essential oil of myracrodruon urundeuva (engl.) fr. all.: composition, cytotoxity and possible mechanisms of action. | myracrodruon urundeuva (engl.) fr. all., commonly known as "aroeira-do-sertão", is a medicinal plant from anacardiaceae family. in this study, the chemical composition of m. urundeuva essential oil (mueo) was evaluated by gas chromatography-mass spectrometry (gc-ms), as well as its anti-leishmania potential, cytotoxicity, and macrophage activation capability as possible antiprotozoal mechanism of action were assessed. fourteen compounds were identified, which constituted 94.87% of total oil comp ... | 2017 | 28189487 |
| n-acetyl-cysteine inhibits liver oxidative stress markers in balb/c mice infected with leishmania amazonensis. | leishmaniasis is a parasitosis caused by several species of the genus leishmania. these parasites present high resistance against oxidative stress generated by inflammatory cells. | 2017 | 28177049 |
| leishmania in synanthropic rodents (rattus rattus): new evidence for the urbanization of leishmania (leishmania) amazonensis. | this study aimed to detect parasites from leishmania genus, to determine the prevalence of anti-leishmania spp. antibodies, to identify circulating species of the parasite, and to determine epidemiological variables associated with infection in rats caught in urban area of londrina, paraná, brazil. animal capture was carried out from may to december 2006, serological and molecular methods were performed. dna was extracted from total blood, and nested-pcr, targeting ssu rrna from leishmania genus ... | 2017 | 28177041 |
| leishmania amazonensis and leishmania chagasi: in vitro leishmanicide activity of virola surinamensis (rol.) warb. | virola species have been used in traditional medicine as healing in skin infections. from v. surinanensis oil were isolated several sesquiterpene as the nerolidol which showed activity against species of leishmania. the current study aimed to evaluate the leishmanicide activity and toxicity of extracts, fractions and surinamesin obtained from leaves of virola surinamensis. hexane, ethyl acetate, and methanol extracts were obtained from powder of dry leaves of v. surinamensis. the hexane and ethy ... | 2017 | 28174103 |
| in vitro and in vivo antimalarial potential of oleoresin obtained from copaifera reticulata ducke (fabaceae) in the brazilian amazon rainforest. | in view of the wide variety of the flora of the amazon region, many plants have been studied in the search for new antimalarial agents. copaifera reticulata is a tree distributed throughout the amazon region which contains an oleoresin rich in sesquiterpenes and diterpenes with β-caryophyllene as the major compound. the oleoresin has demonstrated antiparasitic activity against leishmania amazonensis. because of this previously reported activity, this oleoresin would be expected to also have anti ... | 2017 | 28160850 |
| design and synthesis of a new series of 3,5-disubstituted isoxazoles active against trypanosoma cruzi and leishmania amazonensis. | chagas disease and leishmaniasis are neglected tropical diseases (ntds) endemic in developing countries. although there are drugs available for their treatment, efforts on finding new efficacious therapies are continuous. the natural lignans grandisin (1) and veraguensin (2) show activity against trypomastigote t. cruzi and their scaffold has been used as inspiration to design new derivatives with improved potency and chemical properties. we describe here the planning and microwave-irradiated sy ... | 2017 | 28152426 |
| effect of clinically approved hdac inhibitors on plasmodium, leishmania and schistosoma parasite growth. | malaria, schistosomiasis and leishmaniases are among the most prevalent tropical parasitic diseases and each requires new innovative treatments. targeting essential parasite pathways, such as those that regulate gene expression and cell cycle progression, is a key strategy for discovering new drug leads. in this study, four clinically approved anti-cancer drugs (vorinostat, belinostat, panobinostat and romidepsin) that target histone/lysine deacetylase enzymes were examined for in vitro activity ... | 2017 | 28107750 |
| perifosine mechanisms of action in leishmania species. | here the mechanism by which perifosine induced cell death in leishmania donovani and leishmania amazonensis is described. the drug reduced leishmania mitochondrial membrane potential and decreased cellular atp levels while increasing phosphatidylserine externalization. perifosine did not increase membrane permeabilization. we also found that the drug inhibited the phosphorylation of akt in the parasites. these results highlight the potential use of perifosine as an alternative to miltefosine aga ... | 2017 | 28096161 |
| high dilutions of antimony modulate cytokines production and macrophage - leishmania (l.) amazonensis interaction in vitro. | in previous results mice treated with high dilutions of antimony presented reduction of monocyte migration to the site of infection with increase in b lymphocytes population in the local lymph node. | 2017 | 28092793 |
| vector competence of lutzomyia cruzi naturally demonstrated for leishmania infantum and suspected for leishmania amazonensis. | corumbá city is one of the oldest visceral leishmaniasis-endemic foci in the state of mato grosso do sul, brazil, where the transmission of leishmania infantum has been attributed to lutzomyia cruzi aiming at investigating the parameters of the vectorial capacity of lu. cruzi for l. infantum, a project was undertaken in this city. among these parameters, vector competence was investigated and the results obtained are reported herein. of the 12 hamsters exposed to feed wild-caught female sandflie ... | 2017 | 28077746 |
| phenotypic diversity and selection maintain leishmania amazonensis infectivity in balb/c mouse model. | leishmania are protozoan parasites that show remarkable diversity, as revealed by the various clinical forms of leishmaniasis, which can range from mild skin lesions to severe metastatic cutaneous/mucosal lesions. the exact nature and extent of leishmania phenotypic diversity in establishing infection is not fully understood. in order to try to understand some aspects of this diversity, we subcutaneously infected balb/c mice with first and second generation subclones of a l. amazonensis strain i ... | 2017 | 28076468 |
| development and evaluation of zinc phthalocyanine nanoemulsions for use in photodynamic therapy for leishmania spp. | photodynamic therapy (pdt) combines light with photosensitizers (ps) for production of reactive oxygen species (ros) that can kill infectious microorganisms such as bacteria, fungi and protozoa. the application of nanotechnology has enabled the advancement of pdt because many ps are insoluble in water, necessitating a nanocarrier as a physiologically acceptable carrier. nanoemulsions are efficient nanocarriers for solubilizing liposoluble drugs, like the ps, in water. cutaneous (cl) and mucocuta ... | 2017 | 28071592 |
| in vitro antiprotozoal activity of (s)-cis-verbenol against leishmania spp. and trypanosoma cruzi. | (s)-cis-verbenol, a monoterpene frequently found as a component of essential oils, was assayed against leishmania amazonensis, leishmania infantum, leishmania brasiliensis and against two strains of trypanosoma cruzi. the cytotoxicity of the compound was also assayed against human fibroblast cells using a colorimetric method. benznidazole was used as reference drug against t. cruzi and amphotericin b was used against leishmania spp. the compound showed good activity against the trypanosomes, bei ... | 2017 | 28062234 |
| a recombinant fusion protein displaying murine and human mhc class i- and ii-specific epitopes protects against leishmania amazonensis infection. | tegumentary leishmaniasis (tl) constitutes a major public health problem with significant morbidity worldwide. synthetic peptide-based vaccines are attractive candidates to protect against leishmaniasis, since t cell-specific epitopes can be delivery to antigen-presenting cells, leading to the generation of a th1 cell-mediated immunity. in this context, the present study aims to evaluate the immunogenicity and protective efficacy of a vaccine composed of major histocompatibility complex class i ... | 2017 | 28049560 |
| oligopeptidase b and b2: comparative modelling and virtual screening as searching tools for new antileishmanial compounds. | leishmaniasis are diseases caused by parasites of the genus leishmania and transmitted to humans by the bite of infected insects of the subfamily phlebotominae. current drug therapy shows high toxicity and severe adverse effects. recently, two oligopeptidases (opbs) were identified in leishmania amazonensis, namely oligopeptidase b (opb) and oligopeptidase b2 (opb2). these opbs could be ideal targets, since both enzymes are expressed in all parasite lifecycle and were not identified in human. th ... | 2017 | 28031079 |
| antileishmanial activity of verbascoside: selective arginase inhibition of intracellular amastigotes of leishmania (leishmania) amazonensis with resistance induced by lps plus ifn-γ. | verbascoside is the main component of the traditional medicinal plants that were used against protozoa parasites that cause malaria and leishmaniasis. previously, we have described verbascoside inhibition of leishmania amazonensis arginase as well as its antileishmanial action against extracellular promastigotes. in this study, we have assessed arginase parasite inhibition in intracellular amastigotes. in addition, we verified whether verbascoside can influence the host defense against the paras ... | 2017 | 28017773 |
| leishmanicidal activity of the alkaloid-rich fraction from guatteria latifolia. | leishmaniasis is caused by protozoan parasites belonging to the genus leishmania and includes cutaneous, mucocutaneous and visceral clinical forms. the drugs currently available for leishmaniasis treatment are pentavalent antimonials, amphotericin b and miltefosine, which present high toxicity, elevated cost and development of parasite resistance. the natural products constitute an important source of substances with leishmanicidal potential. here we evaluated in vitro the anti-leishmania amazon ... | 2017 | 28011169 |
| bioactive compounds of aspergillus terreus-f7, an endophytic fungus from hyptis suaveolens (l.) poit. | the compounds terrein (1), butyrolactone i (2), and butyrolactone v (3) were isolated from the ethyl acetate extract (etoac) of the endophytic fungus aspergillus terreus-f7 obtained from hyptis suaveolens (l.) poit. the extract and the compounds presented schistosomicidal activity against schistosoma mansoni; at 100 µg/ml for etoac extract, 1297.3 µm for compound 1, 235.6 µm for compound 2, and 454.1 µm for compound 3, they killed 100% of the parasites after 72 h of treatment. compounds 1, 2, an ... | 2017 | 28243983 |
| biological activities of croton palanostigma klotzsch. | different species of croton are used in traditional amazonian medicine. among the popular uses are treatment of bacterial diseases, poorly healing wounds and fevers. | 2017 | 26246738 |
| treatment of leishmania (leishmania) amazonensis-infected mice with a combination of a palladacycle complex and heat-killed propionibacterium acnes triggers protective cellular immune responses. | palladacycle complex dppe 1.2 was previously reported to inhibit the in vitro and in vivo infection by leishmania (leishmania) amazonensis. the aim of the present study was to compare the effect of dppe 1.2, in association with heat-killed propionibacterium acnes, on l. (l.) amazonensis infection in two mouse strains, balb/c and c57bl/6, and to evaluate the immune responses of the treated animals. foot lesions of l. (l.) amazonensis-infected mice were injected with dppe 1.2 alone, or associated ... | 2017 | 28321209 |
| nanoscaled hydrated antimony (v) oxide as a new approach to first-line antileishmanial drugs. | coordination compounds of pentavalent antimony have been, and remain, the first-line drugs in leishmaniasis treatment for >70 years. molecular forms of sb (v) complexes are commercialized as sodium stibogluconate (pentostam(®)) and meglumine antimoniate (ma) (glucantime(®)). ever-increasing drug resistance in the parasites limits the use of antimonials, due to the low drug concentrations being administered against high parasitic counts. sb(5+) toxicity provokes severe side effects during treatme ... | 2017 | 28008252 |
| antiprotozoal and antioxidant alkaloids from alternanthera littoralis. | five alkaloids, in addition to hydroxytyrosol and uridine, were isolated from aerial parts of alternanthera littoralis p. beauv. among the isolated compounds, alternamide a was an unusual tricyclic alkaloid with a bridged benzoazepine core. all isolated alkaloids have a catechol moiety, indicating a possible common biosynthetic route. their structures were established by 1d and 2d nmr spectroscopy in combination with extensive tandem ms experiments by collisional induced dissociation (cid). the ... | 2017 | 27889243 |
| consequences of acute oxidative stress in leishmania amazonensis: from telomere shortening to the selection of the fittest parasites. | leishmaniasis is a spectrum of diseases caused by parasites of the genus leishmania that affects millions of people around the world. during infection, the parasites use different strategies to survive the host's defenses, including overcoming exposure to reactive oxidant species (ros), responsible for causing damage to lipids, proteins and dna. this damage especially affects telomeres, which frequently results in genome instability, senescence and cell death. telomeres are the physical ends of ... | 2017 | 27836509 |
| anti-leishmania amazonensis activity of serjania lethalis a. st.-hil. | extracts of serjania lethalis a. st.-hil leaves and stems were tested in order to identify potential agents against leishmania amazonensis. the hexane fraction (hf) and dichloromethane subfractions (ddf and mdf) showed leishmanicidal effect. the anti-promastigote ic50 values were 10.29 (hf), 11.41 (ddf) and 28.33μg⁄ml (mdf); whereas those against amastigote were 7.2 (hf), 8.1 (ddf) and 6.5μg⁄ml (mdf). among the fractions and subfractions assayed, only hf altered the cell cycle of the parasite, i ... | 2017 | 27793725 |
| use of recombinant antigens for sensitive serodiagnosis of american tegumentary leishmaniasis caused by different leishmania species. | american tegumentary leishmaniasis (atl) (also known as cutaneous leishmaniasis [cl]) is caused by various species of protozoa of the genus leishmania the diagnosis is achieved on a clinical, epidemiological, and pathological basis, supported by positive parasitological exams and demonstration of leishmanin delayed-type hypersensitivity. serological assays are not routinely used in the diagnosis because many are considered to have low sensitivity and the particular leishmania species causing the ... | 2017 | 27927927 |
| the cytotoxic activity of miltefosine against leishmania and macrophages is associated with dynamic changes in plasma membrane proteins. | in this study, we combined electron paramagnetic resonance (epr) spectroscopy with an analysis of biophysical cellular parameters to study the mechanisms underlying the in vitro anti-leishmanial activity of miltefosine (mt). a thiol-specific spin label attached to membrane-bound proteins of leishmania amazonensis and peritoneal macrophages indicated that mt may bind to plasma membrane proteins in large quantities via a detergent-like action and cause structural changes associated with a marked i ... | 2017 | 27773565 |
| antileishmanial activity of a calophyllum brasiliense leaf extract. | calophyllum brasiliense (clusiaceae) is a tree that grows mainly in the atlantic forest in brazil. its leaves possess antibacterial activity, cytotoxic activity against certain tumor cell lines, and antimicrobial activity in balb/c mice infected with leishmania (leishmania) amazonensis.aiming to identify ultrastructural changes and dna fragmentation in leishmania (viannia) braziliensis, promastigotes were treated with a concentration of the dichloromethane extract and coumarin (-) mammea a/bb fr ... | 2017 | 27224267 |
| leishmania infection inhibits macrophage motility by altering f-actin dynamics and the expression of adhesion complex proteins. | leishmania is an intracellular protozoan parasite that causes a broad spectrum of clinical manifestations, ranging from self-healing skin lesions to fatal visceralizing disease. as the host cells of choice for all species of leishmania, macrophages are critical for the establishment of infections. how macrophages contribute to parasite homing to specific tissues and how parasites modulate macrophage function are still poorly understood. in this study, we show that leishmania amazonensis infectio ... | 2017 | 27641840 |
| in vitro effects of the neolignan 2,3-dihydrobenzofuran against leishmania amazonensis. | leishmaniasis is an infectious disease complex caused by protozoa from the leishmania genus, which presents a broad spectrum of clinical manifestations: cutaneous, mucocutaneous and visceral forms. the current treatments are unsatisfactory considering that few drugs are available and present some level of toxicity. many lignans and neolignans have been used for the development of new antileishmania drugs. the capability in vitro of the neolignan 2,3-dihydrobenzofuran (2,3-dbf), a commonly found ... | 2017 | 27398818 |
| a3k2a3-induced apoptotic cell death of leishmania amazonensis occurs through caspase- and atp-dependent mitochondrial dysfunction. | leishmaniasis is a neglected tropical disease that affects millions of people worldwide. current therapies mainly rely on antimonial drugs that are inadequate because of their high toxicity and increased drug resistance. an urgent need exists to discover new, more effective, more affordable, and more target-specific drugs. pathways that are associated with apoptosis-like cell death have been identified in unicellular eukaryotes, including protozoan parasites. in the present study, we studied the ... | 2017 | 27761752 |
| exploration of the imidazo[1,2-b]pyridazine scaffold as a protein kinase inhibitor. | 3,6-disubstituted imidazo[1,2-b]pyridazine derivatives were synthesized to identify new inhibitors of various eukaryotic kinases, including mammalian and protozoan kinases. among the imidazo[1,2-b]pyridazines tested as kinase inhibitors, several derivatives were selective for dyrks and clks, with ic50 < 100 nm. the characterization of the kinome of several parasites, such as plasmodium and leishmania, has pointed out profound divergences between protein kinases of the parasites and those of the ... | 2017 | 27721154 |
| a vaccine composed of a hypothetical protein and the eukaryotic initiation factor 5a from leishmania braziliensis cross-protection against leishmania amazonensis infection. | in the present study, two proteins cloned from leishmania braziliensis species, a hypothetical protein (lbhyp) and the eukaryotic initiation factor 5a (eif5a), were evaluated to protect balb/c mice against l. amazonensis infection. the animals were immunized with the antigens, either separately or in combination, using saponin as an immune adjuvant in both cases. spleen cells from vaccinated and later infected mice produced significantly higher levels of protein and parasite-specific ifn-γ, il-1 ... | 2017 | 27693018 |
| a proteomic road to acquire an accurate serological diagnosis for human tegumentary leishmaniasis. | diagnostic tools are important for clinical management and epidemiological evaluation of tegumentary (tl) and visceral (vl) leishmaniasis. serology is not frequently used for the diagnosis of the tl form because low antibody titers and cross-reaction with vl. therefore, it is crucial to identify specific and immunogenic antigens from species associated with the tl form. here we employed a proteomic approach coupled to an in silico analysis and identified the most abundant and immunogenic protein ... | 2017 | 27262223 |
| new primers for detection of leishmania infantum using polymerase chain reaction. | leishmania infantum causes visceral leishmaniasis (vl) in the new world. the diagnosis of vl is confirmed by parasitological and serological tests, which are not always sensitive or specific. our aim was to design new primers to perform a polymerase chain reaction (pcr) for detecting l. infantum. sequences of the minicircle kinetoplast dna (kdna) were obtained from genbank, and the flc2/rlc2 primers were designed. samples of dna from l. infantum, leishmania amazonensis, leishmania braziliensis, ... | 2017 | 26603223 |
| leishmania infection in bats from a non-endemic region of leishmaniasis in brazil. | leishmaniasis is a complex of zoonotic diseases caused by parasites of the genus leishmania, which can develop in domestic as well as wild animals and humans throughout the world. currently, this disease is spreading in rural and urban areas of non-endemic regions in brazil. recently, bats have gained epidemiological significance in leishmaniasis due to its close relationship with human settlements. in this study, we investigated the presence of leishmania spp. dna in blood samples from 448 bats ... | 2017 | 28831941 |
| synthesis, in vitro and in vivo giardicidal activity of nitrothiazole-nsaid chimeras displaying broad antiprotozoal spectrum. | we designed and synthesized five new 5-nitrothiazole-nsaid chimeras as analogues of nitazoxanide, using a dcc-activated amidation. compounds 1-5 were tested in vitro against a panel of five protozoa: 2 amitochondriates (giardia intestinalis, trichomonas vaginalis) and 3 kinetoplastids (leishmania mexicana, leishmania amazonensis and trypanosoma cruzi). all chimeras showed broad spectrum and potent antiprotozoal activities, with ic50 values ranging from the low micromolar to nanomolar order. comp ... | 2017 | 28645659 |
| platonia insignis mart., a brazilian amazonian plant: the stem barks extract and its main constituent lupeol exert antileishmanial effects involving macrophages activation. | platonia insignis mart., popularly known as "bacurizeiro," is used in traditional medical practices based on its diverse biological properties. this study was aimed at evaluating the antileishmanial effects of the ethanol extract (etoh-ext), hexane fraction (hex-f), and its main isolated lupeol obtained from stem barks of p. insignis against leishmania (leishmania) amazonensis, as well as their cytotoxicity and possible mechanisms of action. the etoh-ext, hex-f, and lupeol inhibited the growth o ... | 2017 | 28852412 |
| effects of bone marrow mesenchymal stromal cell therapy in experimental cutaneous leishmaniasis in balb/c mice induced by leishmania amazonensis. | cutaneous leishmaniasis remains both a public health and a therapeutic challenge. to date, no ideal therapy for cutaneous leishmaniasis has been identified, and no universally accepted therapeutic regimen and approved vaccines are available. due to the mesenchymal stromal cell (msc) immunomodulatory capacity, they have been applied in a wide variety of disorders, including infectious, inflammatory, and allergic diseases. we evaluated the potential effects of bone marrow msc therapy in a murine m ... | 2017 | 28848541 |
| a calmodulin-like protein (lcala) is a new leishmania amazonesis candidate for telomere end-binding protein. | leishmania spp. telomeres are composed of 5'-ttaggg-3' repeats associated with proteins. we have previously identified larbp38 and larpa-1 as proteins that bind the g-rich telomeric strand. at that time, we had also partially characterized a protein:dna complex, named lagt1, but we could not identify its protein component. | 2017 | 28844976 |
| the photodynamic action of pheophorbide a induces cell death through oxidative stress in leishmania amazonensis. | leishmaniasis is a disease caused by hemoflagellate protozoa, affecting millions of people worldwide. the difficulties of treating patients with this parasitosis include the limited efficacy and many side effects of the currently available drugs. therefore, the search for new compounds with leishmanicidal action is necessary. photodynamic therapy has been studied in the medical field because of its selectivity, utilizing a combination of visible light, a photosensitizer compound, and singlet oxy ... | 2017 | 28821011 |
| synthesis and in vitro evaluation of ca(2+) channel blockers 1,4-dihydropyridines analogues against trypanosoma cruzi and leishmania amazonensis: sar analysis. | drugs containing the1,4-dihydropyridine (dhp) core have recently attracted attention concerning their antiparasitic effect against various species of leishmania and trypanosoma. this approach named drugs repositioning led to interesting results, which have prompted us to prepare 21 dhp's analogues. the 1,4-dhp scaffold was decorated with different function groups at tree points including the nitrogen atom (nh and n-phenyl), the aryl group attached to c-4 (various substituted aryl residues) and t ... | 2017 | 28801098 |
| leishmania amazonensis-induced camp triggered by adenosine a2b receptor is important to inhibit dendritic cell activation and evade immune response in infected mice. | differently from others leishmania species, infection by the protozoan parasite l. amazonensis is associated with a lack of antigen-specific t-cell responses. dendritic cells (dc) are essential for the innate immune response and for directing the differentiation of t-helper lymphocytes. previously, we showed that l. amazonensis infection impairs dc activation through the activation of adenosine a2b receptor, and here, we evaluated the intracellular events triggered by this receptor in infected c ... | 2017 | 28791011 |
| morita-baylis-hillman adducts display anti-inflammatory effects by modulating inflammatory mediator expression in raw264.7 cells. | inflammatory response plays an important role not only in the normal physiology but also in pathologies such as cancers. the morita-baylis-hillman adducts (mbha) are a novel group of synthetic molecules that have demonstrated many biological activities against some parasitic cells such as plasmodium falciparum, leishmania amazonensis, and leishmania chagasi, and antimitotic activity against sea urchin embryonic cells was also related. however, little is known about the mechanisms induced by mbha ... | 2017 | 28785138 |
| dectin-1 activation during leishmania amazonensis phagocytosis prompts syk-dependent reactive oxygen species production to trigger inflammasome assembly and restriction of parasite replication. | protozoan parasites of the genus leishmania are the causative agents of leishmaniasis, a disease that can be lethal and affects 12 million people worldwide. leishmania replicates intracellularly in macrophages, a process that is essential for disease progression. although the production of reactive oxygen species (ros) accounts for restriction of parasite replication, leishmania is known to induce ros upon macrophage infection. we have recently demonstrated nlrp3 inflammasome activation in infec ... | 2017 | 28784846 |
| anti-leishmania activity of new ruthenium(ii) complexes: effect on parasite-host interaction. | leishmaniasis is a parasitic disease caused by protozoa of the genus leishmania. the many complications presented by the current treatment - including high toxicity, high cost and parasite resistance - make the development of new therapeutic agents indispensable. the present study aims to evaluate the anti-leishmania potential of new ruthenium(ii) complexes, cis‑[ru(ii)(η(2)-o2cr)(dppm)2]pf6, with dppm=bis(diphenylphosphino)methane and r=4-butylbenzoate (bbato) 1, 4-(methylthio)benzoate (mtbato) ... | 2017 | 28783554 |
| murine cutaneous leishmaniasis investigated by maldi mass spectrometry imaging. | imaging mass spectrometry (ims) is recognized as a powerful tool to investigate the spatial distribution of untargeted or targeted molecules of a wide variety of samples including tissue sections. leishmania is a protozoan parasite that causes different clinical manifestations in mammalian hosts. leishmaniasis is a major public health risk in different continents and represents one of the most important neglected diseases. cutaneous lesions from mice experimentally infected with leishmania spp. ... | 2017 | 28783195 |
| evaluation of boldine activity against intracellular amastigotes of leishmania amazonensis. | leishmaniasis is a neglected and endemic disease that affects poorest population mainly in developing countries. a lack of adequate and definitive chemotherapeutic agents to fight against this infection has led to the investigation of numerous compounds. the aim of this study was to investigate in vitro activity of boldine against leishmania amazonensis murine cell infection. boldine ((s)-2,9-dihydroxy-1,10-dimethoxy-aporphine) is an aporphine alkaloid found abundantly in the leaves/bark of bold ... | 2017 | 28719960 |
| il-32γ promotes the healing of murine cutaneous lesions caused by leishmania braziliensis infection in contrast to leishmania amazonensis. | interleukin 32 (il-32) is a pro-inflammatory cytokine induced in patients with american tegumentary leishmaniasis (atl) caused by leishmania braziliensis. here, we investigated whether il-32 is also expressed in patient lesions caused by l. amazonensis. in addition, we evaluated experimental l. amazonensis and l. braziliensis infections in c57bl/6 transgenic mice for human il-32γ (il-32γtg) in comparison with wild-type (wt) mice that do not express the il-32 gene. | 2017 | 28709468 |
| chemical characterization, antileishmanial activity, and cytotoxicity effects of the essential oil from leaves of pluchea carolinensis (jacq.) g. don. (asteraceae). | current strategies to control leishmaniasis are mainly based on chemotherapy. however, none of the available drugs can be considered to be ideal to treat this disease. because of the hydrophobic nature and bioactivities of their components, essential oils (eos) can be considered as important sources for developing agents against intracellular pathogens, such as leishmania parasites. in this study, we report the chemical characterization, antileishmanial activities, and cytotoxicity effect of the ... | 2017 | 28703380 |
| kh-tfmdi, a novel sirtuin inhibitor, alters the cytoskeleton and mitochondrial metabolism promoting cell death in leishmania amazonensis. | treatment of leishmaniasis involves the use of antimonials, miltefosine, amphotericin b or pentamidine. however, the side effects of these drugs and the reports of drug-resistant parasites demonstrate the need for new treatments that are safer and more efficacious. histone deacetylase inhibitors are a new class of compounds with potential to treat leishmaniasis. herein, we evaluated the effects of kh-tfmdi, a novel histone deacetylase inhibitor, on leishmania amazonensis promastigotes and intrac ... | 2017 | 28685254 |
| amiodarone and itraconazole improve the activity of pentavalent antimonial in the treatment of experimental cutaneous leishmaniasis. | leishmaniasis affect millions of people, causing morbidity and mortality, especially in developing tropical and subtropical countries. unfortunately, the possibilities of treatment for these infections are still quite limited and most of the available drugs present serious side effects. the objective of this paper was to evaluate the therapeutic role of amiodarone and itraconazole in the treatment of cutaneous leishmaniasis caused by leishmania (leishmania) amazonensis. in order to perform this ... | 2017 | 28668672 |
| the potent cell permeable calpain inhibitor mdl28170 affects the interaction of leishmania amazonensis with macrophages and shows anti-amastigote activity. | since the discovery of the28 first drugs used in leishmaniasis treatment up to now, the search for compounds with anti-leishmania activity without toxic effects and able to overcome the emergency of resistant strains remains a major goal to combat this neglected disease. with this in mind, in the present work, we evaluated the effects of the calpain inhibitor mdl28170 on the interaction process of leishmania amazonensis promastigote forms with murine peritoneal macrophages and on the intracellul ... | 2017 | 28663009 |
| in vitro additive interaction between ketoconazole and antimony against intramacrophage leishmania (leishmania) amazonensis amastigotes. | leishmaniasis is a group of diseases caused by protozoa of leishmania genus. the currently available treatments for this disease are expensive, present high toxicity and are associated to difficulties of healing and parasite resistance. therefore, the development of strategies for leishmaniasis treatment is indispensable and includes reposition of existing drugs, as well as drug combination therapy. the aim of this study was to assess the nature of ketoconazole and antimony association on the cy ... | 2017 | 28662149 |
| lipid-core nanocapsules increase the oral efficacy of quercetin in cutaneous leishmaniasis. | new oral treatments are needed for all forms of leishmaniasis. here, the improved oral efficacy of quercetin (qc) and its penta-acetylated derivative (pqc) was evaluated in cutaneous leishmaniasis after encapsulation in lipid-core nanocapsules (lncs) of poly(ε-caprolactone). leishmania amazonensis-infected balb/c mice were given 51 daily oral doses of free drugs (16 mg kg-1) or lnc-loaded drugs (0·4 mg kg-1). while treatment with free qc reduced the lesion sizes and parasite loads by 38 and 71%, ... | 2017 | 28653597 |
| chemical profile and anti-leishmanial activity of three ecuadorian propolis samples from quito, guayaquil and cotacachi regions. | three propolis samples were collected from different regions of ecuador (quito, guayaquil and cotacachi) and their methanolic extracts were prepared. preliminary information supplied by tlc and nmr data, allowed us to define two main types of propolis: cotacachi propoli sample (cps), rich in flavonoids and quito and guayaquil samples (qps and gps) containing triterpenic alcohols and acetyl triterpenes as the main constituents. two different approaches based on rp-hplc preparative procedure and n ... | 2017 | 28642199 |
| crotoxin stimulates an m1 activation profile in murine macrophages during leishmania amazonensis infection. | american tegumentary leishmaniasis is caused by different species of leishmania. this protozoan employs several mechanisms to subvert the microbicidal activity of macrophages and, given the limited efficacy of current therapies, the development of alternative treatments is essential. animal venoms are known to exhibit a variety of pharmacological activities, including antiparasitic effects. crotoxin (ctx) is the main component of crotalus durissus terrificus venom, and it has several biological ... | 2017 | 28641584 |
| vosalophen: a vanadium complex with a stilbene derivative-induction of apoptosis, autophagy, and efficiency in experimental cutaneous leishmaniasis. | in our previous work, we demonstrated the promising in vitro effect of vosalophen, a vanadium complex with a stilbene derivative, against leishmania amazonensis. its antileishmanial activity has been associated with oxidative stress in l. amazonensis promastigotes and l. amazonensis-infected macrophages. in the present study, the mechanism involved in the death of parasites after treatment with vosalophen, as well as in vivo effect in the murine model cutaneous leishmaniasis, has been investigat ... | 2017 | 28597089 |
| 1,3-bis(aryloxy)propan-2-ols as potential antileishmanial agents. | we describe herein the synthesis and antileishmanial activity of 1,3-bis(aryloxy)propan-2-ols. five compounds (2, 3, 13, 17, and 18) exhibited an effective antileishmanial activity against stationary promastigote forms of leishmania amazonensis (ic50 < 15.0 μm), and an influence of compound lipophilicity on activity was suggested. most of the compounds were poorly selective, as they showed toxicity toward murine macrophages, except 17 and 18, which presented good selective indexes (si ≥ 10.0). ... | 2017 | 28590516 |
| anti-parasitic effect of the diuretic and na+-atpase inhibitor furosemide in cutaneous leishmaniasis. | leishmania amazonensis promastigotes are known to express furosemide (lasix®)-sensitive p-type membrane na+-atpase. in the present study, furosemide activity was studied in intracellular amastigotes and infected balb/c mice to investigate its efficacy in cutaneous leishmaniasis (cl). intracellular parasites, but not macrophages, were found to be sensitive to killing by furosemide (ic50 = 87 µ m vs cc50 ≫ 1000 µ m, respectively). although furosemide did not induce nitric oxide production or intra ... | 2017 | 28583224 |
| discovery of novel inhibitors for leishmania nucleoside diphosphatase kinase (ndk) based on its structural and functional characterization. | nucleoside diphosphate kinases (ndks) are ubiquitous enzymes that catalyze the transfer of the γ-phosphate moiety from an ntp donor to an ndp acceptor, crucial for maintaining the cellular level of nucleoside triphosphates (ntps). the inability of trypanosomatids to synthesize purines de novo and their dependence on the salvage pathway makes ndk an attractive target to develop drugs for the diseases they cause. here we report the discovery of novel inhibitors for leishmania ndk based on the stru ... | 2017 | 28551817 |
| the iron-dependent mitochondrial superoxide dismutase soda promotes leishmania virulence. | leishmaniasis is one of the leading globally neglected diseases, affecting millions of people worldwide. leishmania infection depends on the ability of insect-transmitted metacyclic promastigotes to invade mammalian hosts, differentiate into amastigotes, and replicate inside macrophages. to counter the hostile oxidative environment inside macrophages, these protozoans contain anti-oxidant systems that include iron-dependent superoxide dismutases (sods) in mitochondria and glycosomes. increasing ... | 2017 | 28550086 |
| cytotoxicity and anti-leishmania amazonensis activity of citrus sinensis leaf extracts. | leishmania amazonensis is the main agent of diffuse cutaneous leishmaniasis, a disease characterized by lesional polymorphism and the commitment of skin surface. previous reports demonstrated that the citrus genus possess antimicrobial activity. | 2017 | 28524774 |
| leishmanicidal activity and structure-activity relationships of essential oil constituents. | several constituents of essential oils have been shown to be active against pathogens such as bacteria, fungi, and protozoa. this study demonstrated the in vitro action of ten compounds present in essential oils against leishmania amazonensis promastigotes. with the exception of p-cymene, all evaluated compounds presented leishmanicidal activity, exhibiting ic50 between 25.4 and 568.1 μg ml(-1). compounds with the best leishmanicidal activity presented a phenolic moiety (ic50 between 25.4 and 82 ... | 2017 | 28509873 |
| efficacy of a binuclear cyclopalladated compound therapy for cutaneous leishmaniasis in the murine model of infection with leishmania amazonensis and its inhibitory effect on topoisomerase 1b. | leishmaniasis is a disease found throughout the (sub)tropical parts of the world caused by protozoan parasites of the leishmania genus. despite the numerous problems associated with existing treatments, pharmaceutical companies continue to neglect the development of better ones. the high toxicity of current drugs combined with emerging resistance makes the discovery of new therapeutic alternatives urgent. we report here the evaluation of a binuclear cyclopalladated complex containing pd(ii) and ... | 2017 | 28507113 |
| structurally optimized analogs of the retrograde trafficking inhibitor retro-2cycl limit leishmania infections. | in infected mammalian cells, leishmania parasites reside within specialized compartments called parasitophorous vacuoles (lpvs). we have previously shown that retro-2, a member of a novel class of small retrograde pathway inhibitors caused reduced lpv sizes and lower parasite numbers during experimental l. mexicana sp. infections. the purpose of this study was to determine if structural analogs of retro-2cycl reported to have superior potency in the inhibition of retrograde pathway-dependent phe ... | 2017 | 28505157 |
| a new l-amino acid oxidase from bothrops jararacussu snake venom: isolation, partial characterization, and assessment of pro-apoptotic and antiprotozoal activities. | a new l-amino acid oxidase (laao) from bothrops jararacussu venom (bjussulaao-ii) was isolated by using a three-step chromatographic procedure based on molecular exclusion, hydrophobicity, and affinity. bjussulaao-ii is an acidic enzyme with pi=3.9 and molecular mass=60.36kda that represents 0.3% of the venom proteins and exhibits high enzymatic activity (4884.53u/mg/mim). we determined part of the primary sequence of bjussulaao-ii by identifying 96 amino acids, from which 34 compose the n-termi ... | 2017 | 28495622 |
| experimental mixed infection of leishmania (leishmania) amazonensis and leishmania (l.) infantum in hamsters (mesocricetus auratus). | in south america, visceral leishmaniasis is frequently caused by leishmania infantum and, at an unknown frequency, by leishmania amazonensis. therefore, mixed infections with these organisms are possible. mixed infections might affect the clinical course, immune response, diagnosis, treatment and epidemiology of the disease. here we describe the clinical course of mixed infections with l. amazonensis and l. infantum in a hamster model. we show that mixed infections are associated with more sever ... | 2017 | 28487000 |
| voacamine alters leishmania ultrastructure and kills parasite by poisoning unusual bi-subunit topoisomerase ib. | indole alkaloids possess a large spectrum of biological activities including anti-protozoal action. here we report for the first time that voacamine, isolated from the plant tabernaemontana coronaria, is an antiprotozoal agent effective against a large array of trypanosomatid parasites including indian strain of leishmania donovani and brazilian strains of leishmania amazonensis and trypanosoma cruzi. it inhibits the relaxation activity of topoisomerase ib of l. donovani (ldtop1b) and stabilizes ... | 2017 | 28483460 |
| targeting leishmania amazonensis amastigotes through macrophage internalisation of a hydroxymethylnitrofurazone nanostructured polymeric system. | dextran-coated poly (n-butyl cyanoacrylate) nanoparticles (pbca-nps) were prepared and were evaluated for enhanced delivery of a promising anti-leishmania drug candidate, hydroxymethylnitrofurazone (nfoh), to phagocytic cells. currently available chemotherapy for leishmaniasis, such as pentavalent antimonials, presents low safety and efficacy. furthermore, widespread drug resistance in leishmaniasis is rapidly emerging. to overcome these drawbacks, the use of nanosized delivery systems can reduc ... | 2017 | 28454918 |
| evaluation of vaccinal effectiveness of preparations containing membrane antigens of leishmania (l.) amazonensis in experimental cutaneous leishmaniasis model. | american tegumentary leishmaniasis (atl) is considered a neglected disease, for which an effective vaccine or an efficient diagnosis is not yet available and whose chemotherapeutic arsenal is threatened by the emergence of resistance by etiological agents such as leishmania amazonensis. atl is endemic in poor countries and has a high incidence in brazil. vaccines developed from native parasite fractions have led to the identification of defined antigenic subunits and the development of vaccine a ... | 2017 | 28433944 |
| effect of 1,2,3-triazole salts, non-classical bioisosteres of miltefosine, on leishmania amazonensis. | here, we report the effect of new non-classical bioisosteres of miltefosine on leishmania amazonensis. fifteen compounds were synthesized and the compound dhmtac, containing an acetate anion, a side chain of 10 carbon atoms linked to n-1 and a methyl group linked to n-3, showed high and selective biological activity against l. amazonensis. on the intracellular amastigotes, stages of the parasite related to human disease, the ic50 values were near or similar to the 1.0μm (0.9, 0.8 and 1.0μm on l. ... | 2017 | 28433512 |
| pcr-based identification of trypanosoma lewisi and trypanosoma musculi using maxicircle kinetoplast dna. | trypanosoma lewisi, transmitted by rat fleas, is a widespread pathogen specific to rats with records of human infection cases. its closely related species with global distribution, trypanosoma musculi, is transmitted between mice by ingestion of infected fleas. these trypanosomes are of similar morphology, making it difficult to distinguish them by microscopy. in this study, we have developed a rapid, sensitive and reliable pcr method for the diagnosis of t. lewisi and t. musculi. the t. lewisi- ... | 2017 | 28427957 |
| effect of piplartine and cinnamides on leishmania amazonensis, plasmodium falciparum and on peritoneal cells of swiss mice. | plants of the piperaceae family produce piplartine that was used to synthesize the cinnamides. | 2017 | 28415906 |
| resolvin d1 drives establishment of leishmania amazonensis infection. | previous studies have indicated that the balance between different eicosanoids reflect the intensity of the inflammatory profile in patients with tegumentary leishmaniasis. more recently, pro-resolution lipid mediators have been shown to play critical roles in dampening pathological inflammatory processes to reestablish homeostasis in a diverse range of experimental settings. among these lipid mediator, resolvins from d series have been described as potent anti-inflammatory and immunomodulatory ... | 2017 | 28393908 |
| [cutaneous leishmaniosis: unusual clinical manifestation]. | clinical manifestations of leishmaniasis are diverse and related to the infecting species, its relationship with the environment and the host immune response. a case of late andean cutaneous leishmaniasis with extensive manifestation is presented. the case was confirmed through microbiological and immunological studies; identification was performed by cytochrome b gene sequencing and the species was determined as leishmania (leishmania) amazonensis. the patient was treated with sodium stibogluco ... | 2016 | 25418662 |
| proteomic analysis reveals differentially expressed proteins in macrophages infected with leishmania amazonensis or leishmania major. | cba macrophages effectively control leishmania major infection, yet are permissive to leishmania amazonensis. employing a transcriptomic approach, we previously showed the up-regulation of the genes involved in the classical pathway of macrophage activation in resistant mice. however, microarray analyses do not evaluate changes in gene expression that occur after translation. to circumvent this analytical limitation, we employed a proteomics approach to increase our understanding of the modulati ... | 2016 | 23628411 |
| phosphatidylserine exposure on the surface of leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function. | leishmania amazonensis parasites can cause diverse forms of leishmaniasis in humans and persistent lesions in most inbred strains of mice. in both cases, the infection is characterized by a marked immunosuppression of the host. we previously showed that amastigote forms of the parasite make use of surface-exposed phosphatidylserine (ps) molecules to infect host cells and promote alternative macrophage activation, leading to uncontrolled intracellular proliferation of the parasites. in this study ... | 2016 | 23163958 |
| lysosomal trafficking regulator (lyst). | regulation of vesicle trafficking to lysosomes and lysosome-related organelles (lros) as well as regulation of the size of these organelles are critical to maintain their functions. disruption of the lysosomal trafficking regulator (lyst) results in chediak-higashi syndrome (chs), a rare autosomal recessive disorder characterized by oculocutaneous albinism, prolonged bleeding, severe immunodeficiency, recurrent bacterial infection, neurologic dysfunction and hemophagocytic lympohistiocytosis (hl ... | 2016 | 26427484 |
| evaluation of leishmanicidal and trypanocidal activities of phenolic compounds from calea uniflora less. | the phytochemical study of calea uniflora led to the isolation of nine phenolic compounds identified as noreugenin (1), ethyl caffeate (2), a mixture of butein (3) + orobol (4), α-hydroxy-butein (5), caffeic acid (6), butein 4'-o-glucopyranosyl (7), quercetin 3-o-glucopyranosyl (8) and 3,5-di-o-caffeoylquinic acid (9). the chemical identity of the isolates was established on the basis of nmr and physical data. the chemical shifts of 5 and 7 have been reassigned and all the isolates were tested a ... | 2016 | 25880257 |
| synthesis and antileishmanial activity of natural dehydrodieugenol and its mono- and dimethyl ethers. | the study of chemistry of naturally occurring compounds and the synthesis of their derivatives is fundamentally important for the development of new drugs. in this work, dehydrodieugenol (dhde) was obtained through oxidative coupling of eugenol, promoted by an aqueous mixture of potassium ferricyanide (k3 [fe(cn)6 ]) and nh3 · h2 o. the partial methoxylation of dhde with mei and k2 co3 mainly resulted in the molecular-shaped monomethyl ether (dhde-1meo) and its dimethyl ether derivative (dhde-2 ... | 2016 | 27251851 |
| monitoring the response of patients with cutaneous leishmaniasis to treatment with pentamidine isethionate by quantitative real-time pcr, and identification of leishmania parasites not responding to therapy. | leishmania (viannia) guyanensis is believed to be the principal cause of cutaneous leishmaniasis (cl) in suriname. this disease is treated with pentamidine isethionate (pi), but treatment failure has increasingly been reported. | 2016 | 26648589 |
| antileishmanial and immunomodulatory effects of the essential oil from tetradenia riparia (hochstetter) codd. | cutaneous leishmaniasis usually presents therapeutic resistance to antimonials, and the existing therapies for leishmaniasis have many adverse effects and toxicity. natural products may be regarded as possible candidates for alternative leishmaniasis treatment. the plant tetradenia riparia has shown promise for the treatment of infectious diseases in folk medicine. we evaluated the antileishmanial activity of an essential oil from t. riparia (treo) and the modulatory effects of treo on cytokine ... | 2016 | 26615004 |
| intranasal vaccination with killed leishmania amazonensis promastigotes antigen (laag) associated with caf01 adjuvant induces partial protection in balb/c mice challenged with leishmania (infantum) chagasi - corrigendum. | 2016 | 26521984 | |
| indigenous microbiota and leishmaniasis. | animals are colonized by their indigenous microbiota from the early days of life. the estimated number of associated bacterial cells in humans is around of 10(14) per individual, most of them in the gut. several studies have investigated the microbiota-host relationship, and the use of germfree animals has been an important tool in these studies. these animals, when infected with a pathogen, have shown to be sometimes more resistant and other times more susceptible than conventional animals. lei ... | 2016 | 26407073 |