Publications

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Performance and penetration of laccase and ABTS inks on various printing substrates.Introduction of an enzyme and a colour-forming reagent into paper enables the development of an authenticity indicator. The purpose of this work was to study the performance of Trametes versicolor laccase, TvL, and ABTS, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt, in various printing substrates when printed with inkjet. The printing substrates included pre-coated mechanical paper additionally coated with PVA, silica and latex. The focus was on the bioanalytical perfor ...201222051108
Removal of monomer delignification products by laccase from Trametes versicolor.The influence of a laccase from Trametes versicolor on the removal of phenolic monomers in liquid hot water pretreated wheat straw supernatants (LHW-S) was examined. Beside the total phenol content derived by Folin-Ciocalteu (FC-) assay, phenolic monomers were measured via headspace-solid phase micro-extraction (HS-SPME)/GC-MS. A notable decrease of the phenols was achieved using 0.2 and 0.5U/mL laccase whilst higher dosage showed no improvement. Nearly all kind of monomer phenolic compounds ide ...201222176974
Efficacy of Yun Zhi (Coriolus versicolor) on Survival in Cancer Patients: Systematic Review and Meta-Analysis.Aim: Patients with cancer frequently use herbs along with the conventional medical treatment, hoping to enhance recovery. Mushrooms have an established history of use in traditional oriental therapies. In Asian cultures, mushrooms are combined with herbal mixtures to treat cancer. This systematic review and meta-analysis draw from randomized, placebo-controlled, double-blind trials to assess the efficacy of Yun Zhi (YZ) for survival in cancer patients. Material & Methods: Systematic review and m ...201222185453
effect of onion-type multilamellar liposomes on trametes versicolor laccase activity and stability.trametes versicolor laccase was encapsulated into onion-type, lipid-based multilamellar vesicles (mlvs). when encapsulated, laccase was isolated from the assay medium but was still active once freed from its capsule. the encapsulation efficiency was larger than 65% at 25 °c and 37 °c and decreased to 55% by introducing 140 mm nacl into the buffered medium (ph = 4.5). mlvs were shown to drastically improve both laccase stability and activity. at 25 °c, laccase activity was doubled in the presence ...201222051377
Nitrogen sources of Oligoporus placenta and Trametes versicolor evaluated in a 2(3) experimental plan.Four full-factorial 2(3) experimental plans were applied to evaluate the nitrogen (N) sources of Oligoporus placenta and Trametes versicolor and their interaction with the atmospheric N(2)-assimilating bacterium Beijerinckia acida. The effects of N from peptone, of sapwood and of N from gaseous N(2) on fungal, bacterial and fungal-bacterial activity were investigated. The activities were determined by quantification of biomass, formation of CO(2), consumption of O(2) and laccase activity. The si ...201222208603
effect of coriolus versicolor polysaccharide-b on the biological characteristics of human esophageal carcinoma cell line eca109.to investigate the effect of coriolus versicolor polysaccharide-b (cvps-b) on the biological characteristics of human esophageal carcinoma cell line eca109 in vitro.201223691473
psp activates monocytes in resting human peripheral blood mononuclear cells: immunomodulatory implications for cancer treatment.polysaccharopeptide (psp), from coriolus versicolor, has been used as an adjuvant to chemotherapy, and has demonstrated anti-tumor and immunomodulating effects. however its mechanism remains unknown. to elucidate how psp affects immune populations, we compared psp treatments both with and without prior incubation in phytohaemagglutinin (pha) - a process commonly used in immune population experimentation. we first standardised a capillary electrophoresis fingerprinting technique for psp identific ...201223497877
biodegradation of organics in landfill leachate by immobilized white rot fungi, trametes versicolor bcc 8725.immobilized trametes versicolor bcc 8725 was evaluated for the biodegradation of the organic components of four different types of landfill leachate collected at different time periods and locations from the nonthaburi landfill site of thailand in batch treatment. the effects of carbon source, ammonia and organic loading on colour, biochemical oxygen demand (bod) and chemical oxygen demand (cod) removal, and the reuse of immobilized fungi were investigated. it was found that fungi can remove 78% ...201223437657
synthetic dye decolorization by three sources of fungal laccase.decolorization of six synthetic dyes using three sources of fungal laccase with the origin of aspergillus oryzae, trametes versicolor, and paraconiothyrium variabile was investigated. among them, the enzyme from p. variabile was the most efficient which decolorized bromophenol blue (100%), commassie brilliant blue (91%), panseu-s (56%), rimazol brilliant blue r (rbbr; 47%), congo red (18.5%), and methylene blue (21.3%) after 3 h incubation in presence of hydroxybenzotriazole (hbt; 5 mm) as the l ...201223369690
Treatment of tetracycline antibiotics by laccase in the presence of 1-hydroxybenzotriazole.Tetracycline antibiotics are widely used in human and veterinary medicine; however, residual amounts of these antibiotics in the environment are of concern since they could contribute to selection of resistant bacteria. In this study, tetracycline (TC), chlortetracycline (CTC), doxycycline (DC) and oxytetracycline (OTC) were treated with laccase from the white rot fungus Trametes versicolor in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). High performance liquid chromatography ...201222071243
Effect of Coriolus versicolor supplemented diet on innate immune response and disease resistance in kelp grouper Epinephelus bruneus against Listonella anguillarum.The effect of Coriolus versicolor extract supplemented diets on innate immune response and disease resistance in kelp grouper, Epinephelus bruneus against Listonella anguillarum, is reported. Kelp grouper were divided into four groups of 25 each and fed with C. versicolor enriched diets at 0% (control), 0.01%, 0.1%, and 1.0% level. After 30 days of feeding, all fish were injected interaperitoneally (i.p.) with 50 µl of L. anguillarum (4.7 × 10(7) CFU) to investigate the immune parameters at week ...201222155010
optimization and kinetics evaluation of biodegradation of synthetic azo reactive dye by fungal consortium.wastewater containing direct dyes discharged from various industries, in particular, textile industry, often cause many environmental problems. among the various effluent treatment methods, biological methods found to be cost effective and do not end up in secondary pollutants. in this study, an attempt has been made to study the decolorization of cibacron yellow s-3r, an azo reactive dye by using fungal cultures such as coriolus versicolor, phanerochaete chrysosporium, pleurotus ostreatus, and ...201123505830
Insights into the homocoupling reaction of 4-methylamino benzoic acid mediated by Trametes versicolor laccase.Spectroscopic measurements combined with Density Functional Theory calculations were applied to the characterization of the homocoupling reaction of 4-methylamino benzoic acid mediated by laccase.201121912806
optimization of laccase production by trametes versicolor cultivated on industrial waste.laccases are very interesting biocatalysts for several industrial applications. its production by different white-rot fungi can be stimulated by a variety of inducing substrates, and the use of lignocellulosic wastes or industrial by-products is one of the possible approaches to reduce production costs. in this work, various industrial wastes were tested for laccase production by trametes versicolor mzki g-99. solid waste from chemomechanical treatment facility of a paper manufacturing plant sho ...201121989801
Microbial transformations of diosgenin by the white-rot basidiomycete Coriolus versicolor.Microbial transformation of diosgenin (3ß-hydroxy-5-spirostene) using white-rot fungus Coriolus versicolor afforded four previously unreported polyhydroxylated steroids, 25(R)-spirost-5-en-3ß,7a,15a,21-tetraol (5), 25(R)-spirost-5-en-3ß,7ß,12ß,21-tetrol (6), (25R)-spirost-5-en-3ß,7a,12ß,21-tetraol (7), and (25R)-spirost-5-en-3ß,7ß,11a,21-tetraol (8), along with three known congeners, 25(R)-spirost-5-en-3ß,7ß-diol (2), 25(R)-spirost-5-en-3ß,7ß,21-triol (3), and 25(R)-spirost-5-en-3ß,7ß,12ß-triol ...201121985022
protective effect of coriolus versicolor cultivated in citrus extract against nitric oxide-induced apoptosis in human neuroblastoma sk-n-mc cells.nitric oxide (no) is a reactive free radical and a messenger molecule in many physiological functions. however, excessive no is believed to be a mediator of neurotoxicity. the medicinal plant coriolus versicolor is known to possess anti-tumor and immune-potentiating activities. in this study, we investigated whether coriolus versicolor possesses a protective effect against no donor sodium nitroprusside (snp)-induced apoptosis in the human neuroblastoma cell line sk-n-mc. we utilized 3-(4,5-dimet ...201122110367
Simple fabrication of polymer-based Trametes versicolor laccase for decolorization of malachite green.A highly efficient and stable biocatalyst (denoted D201_Lac) was fabricated by encapsulating Trametes versicolor laccase within a macroporous and strongly basic exchange resin D201 through a simple adsorption process. Transmission electron micrographs and Fourier transform infrared spectra of the resultant D201_Lac proved that nanosized laccase clusters were embedded into the inner nano-pores/channels of D201. As compared to the free laccase, D201_Lac showed enhanced resistance in the pH range o ...201122169216
degradation of carbamazepine by trametes versicolor in an air pulsed fluidized bed bioreactor and identification of intermediates.the paper describes the aerobic degradation of carbamazepine (cbz), an anti-epileptic drug widely found in aquatic environment, from erlenmeyer flask to bioreactor by the white-rot fungus trametes versicolor. in erlenmeyer flask, cbz at approximately 9 mg l(-1) was almost completely eliminated (94%) after 6 d, while at near environmentally relevant concentrations of 50 μg l(-1), 61% of the contaminant was degraded in 7 d. acridone, acridine, 10,11-dihydro-10,11-dihydroxy-cbz, and 10, 11-epoxy-cb ...201122178304
oxidation of galactomannan by laccase plus tempo yields an elastic gel.chemical modifications of galactomannans are applied to improve and/or modify their solubility, rheological and functional properties, but have limited specificity and are often difficult to control. enzymatic reactions, catalyzed under mild process conditions, such as depolymerization, debranching and oxidation, represent a viable and eco-friendly alternative. in this study, we describe oxidation of guar galactomannan primary hydroxyl groups by a fungal laccase using the stable radical tempo as ...201121903143
Biodegradation of tetrabromobisphenol A by oxidases in basidiomycetous fungi and estrogenic activity of the biotransformation products.Tetrabromobisphenol A (TBBPA) degradation was investigated using white rot fungi and their oxidative enzymes. Strains of the Trametes, Pleurotus, Bjerkandera and Dichomitus genera eliminated almost 1 mM TBBPA within 4 days. Laccase, whose role in TBBPA degradation was demonstrated in fungal cultures, was applied to TBBPA degradation alone and in combination with cellobiose dehydrogenase from Sclerotium rolfsii. Purified laccase from Trametes versicolor degraded approximately 2 mM TBBPA within 5 ...201121865031
biological pretreatment with a cellobiose dehydrogenase-deficient strain of trametes versicolor enhances the biofuel potential of canola straw.the use of trametes versicolor as a biological pretreatment for canola straw was explored in the context of biofuel production. specifically, the effects on the straw of a wild-type strain (52j) and a cellobiose dehydrogenase (cdh)-deficient strain (m4d) were investigated. the xylose and glucose contents of the straw treated with 52j were significantly reduced, while only the xylose content was reduced with m4d treatment. lignin extractability was greatly improved with fungal treatments compared ...201121903381
biodegradation of sulfamethazine by trametes versicolor: removal from sewage sludge and identification of intermediate products by uplc-qqtof-ms.degradation of the sulfonamide sulfamethazine (smz) by the white-rot fungus trametes versicolor was assessed. elimination was achieved to nearly undetectable levels after 20 h in liquid medium when smz was added at 9 mg l(-1). experiments with purified laccase and laccase-mediators resulted in almost complete removal. on the other hand, inhibition of smz degradation was observed when piperonilbutoxide, a cytochrome p450-inhibitor, was added to the fungal cultures. uplc-qqtof-ms analysis allowed ...201121944200
Polysaccharide-K augments docetaxel-induced tumor suppression and antitumor immune response in an immunocompetent murine model of human prostate cancer.Advanced castration-resistant prostate cancer has high mortality rates and limited treatment options. Novel therapies are needed to better contend with this disease. Polysaccharide-K® (PSK), an extract of the mushroom Trametes versicolor, has immunomodulatory and tumor suppressive activities. PSK is used in Asia as a cancer immunotherapy. However, its benefit in combination with taxanes for prostate cancer is unknown. We examined whethe ...201122159900
Enzyme-Catalyzed Oxidation of 17ß-Estradiol Using Immobilized Laccase from Trametes versicolor.Many natural and synthetic estrogens are amenable to oxidation through the catalytic action of oxidative enzymes such as the fungal laccase Trametes versicolor. This study focused on characterizing the conversion of estradiol (E(2)) using laccase that had been immobilized by covalent bonding onto silica beads contained in a bench-scale continuous-flow packed bed reactor. Conversion of E(2) accomplished in the reactor declined when the temperature of the system was changed from room temperature t ...201121869925
degradation of the antibiotics norfloxacin and ciprofloxacin by a white-rot fungus and identification of degradation products.more than 90% of the antibiotics ciprofloxacin (cipro) and norfloxacin (nor) at 2 mg l(-1) were degraded by trametes versicolor after 7 days of incubation in malt extract liquid medium. in in vitro assays with purified laccase (16.7 nkat ml(-1)), an extracellular enzyme excreted constitutively by this fungus, 16% of cipro was removed after 20 h. the addition of the laccase mediator 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt led to 97.7% and 33.7% degradation of cipro a ...201121937221
Isolation and evaluation of terrestrial fungi with algicidal ability from Zijin Mountain, Nanjing, China.Approximately 60 fungal isolates from Zijin Mountain (Nanjing, China) were screened to determine their algicidal ability. The results show that 8 fungi belonging to Ascomycota and 5 belonging to Basidiomycota have algicidal ability. Of these fungi, Irpex lacteus T2b, Trametes hirsuta T24, Trametes versicolor F21a, and Bjerkandera adusta T1 showed strong algicidal ability. The order of fungal chlorophyll-a removal efficiency was as follows: T. versicolor F21a > I. lacteus T2b > B. adusta T1 > T. ...201121887638
azo dye decolorization by a laccase/mediator system in a membrane reactor: enzyme and mediator reusability.this paper presents the use of a membrane-integrated reactor system with recycling of laccase and mediator for azo dye decolorization. from initial screening of different laccases and mediators, trametes versicolor laccase and syringaldehyde provided the best system for decolorization. decolorization yields of 98, 88, 80 and 78% were obtained for red fn-2bl, red bws, remazol blue rr and blue 4bl, respectively. the reaction parameters were optimized and a membrane reactor was set up for dye decol ...201122112621
bioactive metabolites from biotransformation of paeonol by the white-rot basidiomycete coriolus versicolor.biotransformation of paeonol (1) with the white-rot basidiomycete coriolus versicolor afforded two metabolites, 2,4-dihydroxyacetophenone (2) and 2,5-dihydroxy-4-methoxyacetophenone (3), which were identified by spectroscopic methods. compound 3 showed higher antioxidative, antibacterial, antifungal activities than 1 or 2. the results demonstrate for the first time that c. versicolor has the capacities to catalyze hydroxylation and demethylation reactions on the aromatic compound.201121922916
protein-bound polysaccharide-k (psk) induces apoptosis and inhibits proliferation of promyelomonocytic leukemia hl-60 cells.protein-bound polysaccharide-k (psk) is extracted from coriolus versicolor (cm101), and is clinically used in combination therapy for gastrointestinal cancer and small cell lung carcinoma. psk is a biological response modifier (brm), and its mechanism of action is partly mediated, by modulating host immune systems, such as the activation of immune effector cells and the neutralization of transforming growth factor-beta (tgfβ) activity. direct inhibition of tumor cell proliferation has been repor ...201121868514
gc-ms and spectrophotometric analysis of biodegradation of new disazo dye by trametes versicolor.in this study; sub-tropical white rot fungi, trametes versicolor was investigated for its ability to degrade 4-(3'-methyl-4'-(4"-nitrophenyl)azo- 1'h-pyrazol-5'-ylazo)-3-methyl- h-pyrazol-5-on in the mediums containing glucose and different concentrations of degrade dye in batch systems. this dye was synthetized at pamukkale universtiy of organic chemistry research laboratory. samples were collected on 10 days, and was detected by shimadzu uv-1600a spectrophotometry. decolorization study showed ...201122232902
batch production of pyranose 2-oxidase from trametes versicolor (atcc 11235) in medium with a lignocellulosic substrate and enzymatic bleaching of cotton fabrics.the aim of this work was to determine new, different and low-cost substrates that can be used for enzyme production from the white rot fungus trametes versicolor (atcc 11235) by taking advantage of the broad substrate specificity of pyranose 2-oxidase. in this report, we investigated the production of pyranose 2-oxidase from t. versicolor (atcc 11235) using ten different agricultural residues such as clover straw, almond shells, hazelnut cobs, grass and others. pyranose 2-oxidase activity was de ...201122805934
evaluation of ten wild nigerian mushrooms for amylase and cellulase activities.amylases and cellulases are important enzymes that can be utilized for various biological activities. ten different wild nigerian mushrooms (agaricus blazei, agaricus sp., corilopsis occidentalis, coriolus versicolor, termitomyces clypeatus, termitomyces globulus, pleurotus tuber-regium, podoscypha bolleana, pogonomyces hydnoides, and nothopanus hygrophanus) were assayed for production of these secondary metabolites. the results revealed that most of the tested wild fungi demonstrated very good ...201122783085
pushing the limits of automatic computational protein design: design, expression, and characterization of a large synthetic protein based on a fungal laccase scaffold.the de novo engineering of new proteins will allow the design of complex systems in synthetic biology. but the design of large proteins is very challenging due to the large combinatorial sequence space to be explored and the lack of a suitable selection system to guide the evolution and optimization. one way to approach this challenge is to use computational design methods based on the current crystallographic data and on molecular mechanics. we have used a laccase protein fold as a scaffold to ...201122654993
Cloning of novel cellulases from cellulolytic fungi: Heterologous expression of a family 5 glycoside hydrolase from Trametes versicolor in Pichia pastoris.Total cDNA isolated from cellulolytic fungi cultured in cellulose was examined for the presence of sequences encoding for endoglucanases. Novel sequences encoding for glycoside hydrolases (GHs) were identified in Fusarium oxysporum, Ganoderma applanatum and Trametes versicolor. The cDNA encoding for partial sequences of GH family 61 cellulases from F. oxysporum and G. applanatum shares 58 and 68% identity with endoglucanases from Glomerella graminicola and Laccaria bicolor, respectively. A new G ...201122142722
morphology and laccase production of white-rot fungi grown on wheat bran flakes under semi-solid-state fermentation conditions.in this paper, we studied the laccase production and the growth morphology of different white-rot fungi, i.e. pleurotus ostreatus, trametes pubescens, cerrena unicolor and trametes versicolor, cultured under semi-solid-state fermentation conditions using wheat bran flakes as a natural low-cost support substrate. trametes versicolor exhibited the highest laccase activity per gram of total dry matter, followed by p. ostreatus (63.5 and 58.2ug(-1) , respectively). in addition, they showed a time pr ...201121291496
In situ encapsulation of laccase in nanofibers by electrospinning for development of enzyme biosensors for chlorophenol monitoring.A biosensor based on Trametes versicolor laccase (Lac) was developed for the determination of phenolic compounds. The biosensor was prepared by in situ electrospinning of a mixture of polyvinyl alcohol (PVA), Lac, PEO-PPO-PEO (F108) and gold nanoparticles (Au NPs), where F108 was used as an enzyme stabilizing additive and Au NPs was used to enhance the conductivity of the biosensor. Laser confocal scanning microscopy and electrochemical impedance spectroscopy proved that the enzyme was successfu ...201121961111
a highly efficient buckypaper-based electrode material for mediatorless laccase-catalyzed dioxygen reduction.the redox enzyme laccase from trametes versicolor efficiently catalyzes the oxygen reduction reaction (orr) in mediatorless biofuel cell cathodes when adsorbed onto multi-walled carbon nanotubes (mwcnts). in this work we demonstrate that the fabrication of mwcnts in form of buckypaper (bp) results in an excellent electrode material for laccase-catalyzed cathodes. bps are mechanically stable, self-entangling mats with high dispersion of mwcnts resulting in easy to handle homogeneous layers with h ...201121543222
treatment of a textile effluent from dyeing with cochineal extracts using trametes versicolor fungus.trametes versicolor (tv) fungus can degrade synthetic dyes that contain azo groups, anthraquinone, triphenylmethane polymers, and heterocyclic groups. however, no references have been found related to the degradation of natural dyes, such as the carminic acid that is contained in the cochineal extract. experiments to determine the decolorization of the effluent used in the cotton dyeing process with cochineal extract by means of tv fungus were done. treatments to determine decolorization in the ...201121552764
fungal solid state fermentation on agro-industrial wastes for acid wastewater decolorization in a continuous flow packed-bed bioreactor.this study was aimed at developing a process of solid state fermentation (ssf) with the fungi pleurotus ostreatus and trametes versicolor on apple processing residues for wastewater decolorization. both fungi were able to colonize apple residues without any addition of nutrients, material support or water. p. ostreatus produced the highest levels of laccases (up to 9u g(-1) of dry matter) and xylanases (up to 80u g(-1) of dry matter). a repeated batch decolorization experiment was set up with ap ...201121652205
studies of laccase from trametes versicolor in aqueous solutions of several methylimidazolium ionic liquids.stability and kinetic behavior of laccase from trametes versicolor in the presence of several ionic liquids from the methylimidazolium family have been investigated. in general laccase stability diminished as the size of the alkylic substitute in the methylimidazolium ring increased. higher concentrations of ionic liquids caused more destabilization than lower ones. thus, low concentrations of [c(2)mim(+)][etso(4)(-)] allowed maintaining enzymatic stability. [c(4)mim(+)][cl(-)] appeared to have ...201121669518
coriolus versicolor suppresses inflammatory bowel disease by inhibiting the expression of stat1 and stat6 associated with ifn-? and il-4 expression.to investigate the effects of coriolus versicolor extract (cve) on infl?ammatory bowel disease (ibd), ulcerative colitis was induced in male balb/c mice by administering drinking water containing dextran-sulfate sodium (dss). the mice were divided into the following four experimental groups: control, dss-induced colitis, cve treatment and cve treatment + dss-induced colitis. mice receiving dss treatment developed clinical and macroscopic signs of ulcerative colitis. however, treatment with cve r ...201121796702
chemical composition and natural durability of juvenile and mature heartwood of robinia pseudoacacia l.the aim of this study was to characterize the properties of juvenile and mature heartwood of robinia pseudoacacia l. (black locust). the content, the composition, and subcellular localization of heartwood extractives were studied in 14 old-grown trees from forest sites in germany and hungary, as well as in 16 younger trees of four clone types. heartwood extractives (methanol and acetone extraction) were analysed by hplc-chromatography. uv microspectrophotometry was used to localize the extractiv ...201121779654
acute and subchronic oral toxicity of coriolus versicolor standardized water extract in sprague-dawley rats.aim of the study: coriolus versicolor, which is known as yun zhi, is one of the commonly used chinese medicinal herbs. recent studies have demonstrated its antitumor activities on cancer cells which led to its widespread use in cancer patient. however, little toxicological information is available regarding its safety. the present study evaluated the potential toxicity of coriolus versicolor standardized water extract after acute and subchronic administration in rats. material and method: in acu ...201121767625
geometric distortions on a three-coordinated t1 cu site model as a potential strategy to modulate redox potential. a theoretical study.a model of the three-coordinated t1 cu site from trametes versicolor was considered to evaluate the effect on redox potential of geometrical distortions in the copper coordination sphere. systematic modifications of geometrical parameters (distances and angles) of the coordination sphere of the t1 cu site were carried out within a density functional theory (dft) framework, to evaluate their effects on electron affinity directly related to redox potential. the most promising result in terms of re ...201121541748
polysaccharide peptides from coriolus versicolor competitively inhibit tolbutamide 4-hydroxylation in specific human cyp2c9 isoform and pooled human liver microsomes.polysaccharide peptide (psp), isolated from cov-1 strain of coriolus versicolor, is commonly used as an adjunct in cancer chemotherapy in china. previous studies have shown that psp decreased antipyrine clearance and inhibited cyp2c11-mediated tolbutamide 4-hydroxylation in the rat both in vitro and in vivo. in this study, the effects of water extractable fraction of psp on tolbutamide 4-hydroxylation was investigated in pooled human liver microsomes and in specific human cyp2c9 isoform. psp (2. ...201121757329
utilizing pigment-producing fungi to add commercial value to american beech (fagus grandifolia).american beech (fagus grandifolia) is an abundant, underutilized tree in certain areas of north america, and methods to increase its market value are of considerable interest. this research utilized pigment-producing fungi to induce color in american beech to potentially establish its use as a decorative wood. wood samples were inoculated with trametes versicolor, xylaria polymorpha, inonotus hispidus, and arthrographis cuboidea to induce fungal pigmentation. black pigmentation (t. versicolor, x ...201121931972
phytochemicals from cunninghamia konishii hayata act as antifungal agents.the aims of the present study were to isolate and identify the antifungal compounds from the ethanolic extract of cunninghamia konishii wood and to evaluate their antifungal activities against wood decay fungi. the results showed that the n-hex soluble fraction of the ethanolic extract from c. konishii wood had an excellent inhibitory effect against lenzites betulina, trametes versicolor, laetiporus sulphureus, and gloeophyllum trabeum, with ic(50) values of 33, 46, 62, and 49 μg/ml, respectivel ...201122129092
Laccase chloride inhibition reduction by an anthraquinonic substrate.Due to their low substrate specificity, fungal laccases have a great potential in industrial applications, including the bioremediation of colored wastewaters from textile industry. However, the presence of halides in these effluents (up to 1M NaCl) which inhibit laccases is a drawback for bioremediation processes. In order to develop an efficient enzymatic remediation process for textile dye effluent, the possibility to reduce this halide inhibition is conditioned by a better understanding of t ...201122142726
anti-protein-bound polysaccharide-k monoclonal antibody binds the active structure and neutralizes direct antitumor action of the compound.protein-bound polysaccharide k (psk) is extracted and purified from coriolus versicolor (cm101), and is used as an anti-cancer agent. in this study, focusing on the direct actions of psk, we investigated whether psk reaches tumor and immune tissues with its active structure remaining intact, and the direct action of psk was evaluated by its antitumor effects against metha fibrosarcomas implanted in immunodeficient nod/scid mice. the results obtained suggest that psk reaches the tumor tissue in i ...201121249323
wood decomposing abilities of diverse lignicolous fungi on nondecayed and decayed beech wood.we tested the decay abilities of 28 isolates from 28 lignicolous fungal species (basidiomycota, ascomycota and zygomycota) with the pure culture test. we used beech wood powder in varying moisture conditions and decay stages (nondecayed, intermediately decayed and well decayed) as substrates. the weight loss in wood powder was -0.2-17.8%. five isolates of basidiomycota (bjerkandera adusta, mycena haematopus, omphalotus guepiniformis, trametes hirsuta, trametes versicolor) caused high weight loss ...201121262989
purification and characterization of a novel immunomodulatory protein from the medicinal mushroom trametes versicolor.bioactive proteins represent an important group of functional agents in medicinal mushrooms. trametes versicolor (l.) lloyd is a mushroom frequently used in traditional chinese medicine for its anti-tumor and immunomodulatory activities. a new immunomodulatory protein from t. versicolor, named tvc, was purified by ammonium sulfate precipitation, ion-exchange chromatography and gel filtration chromatography. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the purified protei ...201121373981
composition and antifungal activities of the leaf essential oil of neolitsea parvigemma from taiwan.the hydrodistillated leaf essential oil of neolitsea parvigemma was analyzed to determine its composition and yield. sixty-two compounds were identified, the main components being beta-caryophyllene (14.2%), beta-eudesmol (12.9%), alpha-cadinol (10.2%) and tau-cadinol (8.8%). oxygenated sesquiterpenes (48.9%) and sesquiterpene hydrocarbons (48.8%) were the predominant groups of compounds. the antifungal indexes of the leaf oil against the 7 fungi, aspergillus clavatus, a. niger, chaetomium globo ...201121941915
immobilization of laccase on polymer grafted polytetrafluoroethylene membranes for biosensor construction.in this study, trametes versicolor laccase was immobilized on polytetrafluoroethylene (ptfe) membranes using two different techniques, entrapment to gelatin and covalent immobilization to the surface. for surface immobilization, functional groups were formed on ptfe surface by radiofrequency (rf) plasma treatment followed by polymer grafting. two different polymers, polyacrylamide (paam) and polyacrylic acid (paac) were tried. for polyacrylamide grafted ptfe, a two-step polymerization process wa ...201121376983
evaluation of the potential of fungal and plant laccases for active-packaging applications.laccases from trametes versicolor (tvl), myceliophthora thermophila (mtl), and rhus vernicifera (rvl) were investigated with regard to their potential utilization as oxygen scavengers in active packages containing food susceptible to oxidation reactions. the substrate selectivity of the laccases was investigated with a set of 17 reducing substrates, mainly phenolic compounds. the temperature dependence of reactions performed at low temperatures (4-31 °c) was studied. furthermore, the laccases we ...201121524087
TLR2 agonist PSK activates human NK cells and enhances the antitumor effect of HER2-targeted monoclonal antibody therapy.The therapeutic effect of trastuzumab monoclonal antibody (mAb) therapy has been shown to be partially dependent on functional natural killer (NK) cells. Novel agents that enhance NK cell function could potentially improve the antitumor effect of trastuzumab. We recently identified polysaccharide krestin (PSK), a natural product extracted from medicinal mushroom Trametes versicolor, as a potent toll-like receptor 2 (TLR2) agonist. This study was undertaken to evaluate the effect of PSK on human ...201121918170
mild and highly flexible enzyme-catalyzed modification of poly(ethersulfone) membranes.poly(ethersulfone) (pes) membranes are widely used in industry for separation and purification purposes. however, the drawback of this type of membranes is fouling by proteins. for that reason, modification of pes membranes has been studied to enhance their protein repellence. this paper presents the first example of enzyme-catalyzed modification of pes membranes. various phenolic acids (enzyme substrates) were bound to a membrane under very mild conditions (room temperature, water, nearly neutr ...201121344870
solid-phase treatment with the fungus trametes versicolor substantially reduces pharmaceutical concentrations and toxicity from sewage sludge.for safe biosolid-land-applying, sludge should be contaminant-free. however, it may contain important amounts of micropollutants, not removed in the wastewater-treatment-processes. an alternative treatment with the fungus trametes versicolor was applied in sterile solid-phase systems consisting of sludge and a lignocellulosic substrate. fungal colonization and activity were demonstrated during the process, according to monitoring of ergosterol, laccase activity and the naproxen-degradation test ...201121376580
optimisation of the operational conditions of trichloroethylene degradation using trametes versicolor under quinone redox cycling conditions using central composite design methodology.extracellular radicals produced by trametes versicolor under quinone redox cycling conditions can degrade a large variety of pollutant compounds, including trichloroethylene (tce). this study investigated the effect of the agitation speed and the gas-liquid phase volume ratio on tce degradation using central composite design (ccd) methodology for a future scale-up to a reactor system. the agitation speed ranged from 90 to 200 rpm, and the volume ratio ranged from 0.5 to 4.4. the results demonstr ...201121894452
28-day oral safety evaluation of extracellular polysaccharopeptides produced in submerged culture from the turkey tail medicinal mushroom Trametes versicolor (L.:Fr.) Pilát LH-1 in mice.Turkey tail medicinal mushroom, Trametes versicolor (TV), is a species with a variety of pharmacological activities. Its intracellular polysaccharopeptides are widely commercialized. Recently, we found a novel TV strain LH-1 in Taiwan and demonstrated that the extracellular polysaccharopeptide (ePSP) of LH-1 obtained from submerged culture exhibits significant immunomodulatory activity. In this in vivo study, we further evaluated the safety of orally administered LH-1 ePSP using both male and fe ...201122135874
design parameters for tuning the type 1 cu multicopper oxidase redox potential: insight from a combination of first principles and empirical molecular dynamics simulations.the redox potentials and reorganization energies of the type 1 (t1) cu site in four multicopper oxidases were calculated by combining first principles density functional theory (qm) and qm/mm molecular dynamics (md) simulations. the model enzymes selected included the laccase from trametes versicolor, the laccase-like enzyme isolated from bacillus subtilis, cueo required for copper homeostasis in escherichia coli, and the small laccase (slac) from streptomyces coelicolor. the results demonstrate ...201121388209
suppression of growth and invasive behavior of human prostate cancer cells by prostacaid™: mechanism of activity.since the use of dietary supplements as alternative treatments or adjuvant therapies in cancer treatment is growing, a scientific verification of their biological activity and the detailed mechanisms of their action are necessary for the acceptance of dietary supplements in conventional cancer treatments. in the present study we have evaluated the anti-cancer effects of dietary supplement prostacaid™ (pc) which contains mycelium from medicinal mushrooms (ganoderma lucidum, coriolus versicolor, p ...201121468543
efficient removal of pollutants from olive washing wastewater in bubble-column bioreactor by trametes versicolor.the white-rot fungi panus tigrinus, funalia trogii and trametes versicolor have been tested in shake flasks for the reduction of olive washing wastewater (oww) pollutants and production of oxidases on oww-based media. p. tigrinus was rejected for its scarce performance. f. trogii showed best production of laccase (27000ug(-1)), while t. versicolor appeared a good pollutant degrader reducing colour, cod and phenols by 60, 72 and 87%, respectively. only t. versicolor grew well in bubble-column bio ...201121524782
how is the reactivity of laccase affected by single-point mutations? engineering laccase for improved activity towards sterically demanding substrates.in spite of its broad specificity among phenols, trametes versicolor laccase hardly succeeds in oxidizing hindered substrates. to improve the oxidation ability of this laccase towards bulky phenolic substrates, we designed a series of single-point mutants on the basis of the amino-acid layout inside the reducing substrate active site known from the crystal structure of the enzyme. site-directed mutagenesis has addressed four phenylalanine residues in key positions 162, 265, 332, and 337 at the e ...201121468703
coarsening of extracellularly biosynthesized cadmium crystal particles induced by thioacetamide in solution.a novel coarsening route for extracellularly biosynthesized cadmium nanocrystals was investigated for the first time. in this process, the white rot fungus coriolus versicolor was employed to take up cadmium ions and synthesize extracellular cadmium crystal particles. the coarsening of the particles was induced by thioacetamide under certain conditions. scanning electron microscopy showed that the formed cadmium crystal particles were coarsened from about 100nm to 2-3μm. the corresponding energy ...201121489598
a permeable cuticle is associated with the release of reactive oxygen species and induction of innate immunity.wounded leaves of arabidopsis thaliana show transient immunity to botrytis cinerea, the causal agent of grey mould. using a fluorescent probe, histological staining and a luminol assay, we now show that reactive oxygen species (ros), including h(2)o(2) and o(2) (-), are produced within minutes after wounding. ros are formed in the absence of the enzymes atrboh d and f and can be prevented by diphenylene iodonium (dpi) or catalase. h(2)o(2) was shown to protect plants upon exogenous application. ...201121829351
ISTA 14-Impact of antibiotics from pig slurry on soil microbial communities, including the basidiomycete Trametes versicolor.Livestock slurry containing antibiotics is a source of contamination of agricultural soils, with possible effects on soil function and micro-organisms. Extracellular oxido-reductases and hydrolases from the fungus T. versicolor and fungal growth were monitored in liquid cultures in the presence of tetracycline, lincomycine, sulfadiazine and ciprofloxacin for 10 days, in order to assess the suitability of these enzymes as biomarkers. Among the conditions of treatment, statistical analysis demonst ...201121702076
enzyme production by solid substrate fermentation of pleurotus ostreatus and trametes versicolor on tomato pomace.a process of solid state fermentation (ssf) on tomato pomace was developed with the white-rot fungi pleurotus ostreatus and trametes versicolor, using sorghum stalks as support. operative parameters (humidity, water activity, and size of substrate particles) guaranteeing a good colonization of tomato pomace by both fungi were defined and conditions for production at high titers of the industrially relevant enzymes laccase, xylanase and protease were identified. significant laccase activity level ...201120582639
utilization of horticultural waste for laccase production by trametes versicolor under solid-state fermentation.horticultural waste collected from a landscape company in singapore was utilized as the substrate for the production of laccase under solid-state fermentation by trametes versicolor. the effects of substrate particle size, types of inducers, incubation temperature and time, initial medium ph value, and moisture content on laccase production were investigated. the optimum productivity of laccase (8.6 u/g substrate) was achieved by employing horticultural waste of particle size greater than 500 μm ...201120640894
printing of polymer microcapsules for enzyme immobilization on paper substrate.poly(ethyleneimine) (pei) microcapsules containing laccase from trametes hirsuta (thl) and trametes versicolor (tvl) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. microcapsules were fabricated via interfacial polycondensation of pei with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. the same ink components were used for three printing methods, and it was found that lacc ...201121568314
chemopreventive effect of psp through targeting of prostate cancer stem cell-like population.recent evidence suggested that prostate cancer stem/progenitor cells (csc) are responsible for cancer initiation as well as disease progression. unfortunately, conventional therapies are only effective in targeting the more differentiated cancer cells and spare the cscs. here, we report that psp, an active component extracted from the mushroom turkey tail (also known as coriolus versicolor), is effective in targeting prostate cscs. we found that treatment of the prostate cancer cell line pc-3 wi ...201121603625
potential for bioremediation of agro-industrial effluents with high loads of pesticides by selected fungi.wastewaters from the fruit packaging industry contain a high pesticide load and require treatment before their environmental discharge. we provide first evidence for the potential bioremediation of these wastewaters. three white rot fungi (wrf) (phanerochaete chrysosporium, trametes versicolor, pleurotus ostreatus) and an aspergillus niger strain were tested in straw extract medium (stem) and soil extract medium (sem) for degrading the pesticides thiabendazole (tbz), imazalil (imz), thiophanate ...201120635121
biotechnological strategies to overcome inhibitors in lignocellulose hydrolysates for ethanol production: review.one of the major challenges faced in commercial production of lignocellulosic bioethanol is the inhibitory compounds generated during the thermo-chemical pre-treatment step of biomass. these inhibitory compounds are toxic to fermenting micro-organisms. the ethanol yield and productivity obtained during fermentation of lignocellulosic hydrolysates is decreased due to the presence of inhibiting compounds, such as weak acids, furans and phenolic compounds formed or released during thermo-chemical p ...201120513164
effect of aromatic compounds on the production of laccase and manganese peroxidase by white-rot basidiomycetes.three white-rot fungi displayed a wide diversity in their response to supplemented aromatic compounds. pyrogallol stimulated cerrena unicolor laccase and manganese peroxidase (mnp) synthesis in synthetic medium 2.5- and 2-fold, respectively, whereas 2,4,6-trinitrotoluene (tnt) brought about a 2.8-fold increase in laccase yield by trametes versicolor in submerged fermentation of ethanol production residue. no effect of the tested aromatic compounds on enzyme secretion by ganoderma lucidum in mann ...201020532947
new fungal biomasses for cyanide biodegradation.cyanide, a hazardous substance, is released into the environment as a result of natural processes of various industrial activities which is a toxic pollutant according to environmental protection agency. in nature, some microorganisms are responsible for the degradation of cyanide, but there is only limited information about the degradation characteristics of basidiomycetes for cyanide. the aim of the present study is to determine cyanide degradation characteristics in some basidiomycetes strain ...201020547364
immobilization strategies for laccase from trametes versicolor on mesostructured silica materials and the application to the degradation of naphthalene.the oxidation of naphthalene by immobilized laccase from trametes versicolor has been performed using diverse immobilization strategies on mesostructured silica materials. laccase was immobilized by physical adsorption on several sba-15 with different textural properties and by covalent attachment on functionalized sba-15 prepared by co-condensation method (direct synthesis). the adsorption of laccase was partially reversible and showed some degree of lixiviation. however, covalently attached la ...201020599376
elimination of carbamazepine by repeated treatment with laccase in the presence of 1-hydroxybenzotriazole.carbamazepine (cbzp) is used as an antiepileptic drug and is highly persistent. in this study, cbzp was treated with laccase from white rot fungus trametes versicolor in the presence of a redox mediator 1-hydroxybenzotriazole (hbt). a single treatment with laccase and hbt eliminated cbzp by about 22% after 24h, and repeated treatments with laccase and hbt, which were added to the reaction mixture every 8h, helped eliminate about 60% of cbzp after 48h. this suggests that repeated treatment is eff ...201020619797
white-rot fungal conversion of wheat straw to energy rich cattle feed.in order to improve the digestibility and nutrient availability in rumen, wheat straw was subjected to solid state fermentation (ssf) with white-rot fungi (i.e. pleurotus ostreatus and trametes versicolor) and the fermented biomass (called myco-straw) was evaluated for biochemical, enzymatic and nutritional parameters. the fungal treatment after 30 days led to significant decrease (p < 0.05) in cell wall constituents viz, acid detergent fiber (adf), neutral detergent fiber (ndf), hemicellulose, ...201020734121
advanced oxidation of benzene, toluene, ethylbenzene and xylene isomers (btex) by trametes versicolor.advanced oxidation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylene (btex) by the extracellular hydroxyl radicals (*oh) generated by the white-rot fungus trametes versicolor is for the first time demonstrated. the production of *oh was induced by incubating the fungus with 2,6-dimethoxy-1,4-benzoquinone (dbq) and fe3+-edta. under these conditions, *oh were generated through dbq redox cycling catalyzed by quinone reductase and laccase. the capability of t. versicolor growing in malt ex ...201020627409
carbon electrodes for direct electron transfer type laccase cathodes investigated by current density-cathode potential behavior.direct electron transfer from carbon electrodes to adsorbed laccase (ec 1.10.3.2) from trametes versicolor is widely used to enable mediatorless enzymatic biofuel cell cathodes. however, data published so far are poorly comparable in terms of oxygen reduction performance. we thus present a comparative characterization of carbon-based electrode materials as cathode in half-cell configuration, employing adsorbed laccase as oxygen reduction catalyst. open circuit potentials and performances were si ...201020627511
influence of iron and copper nanoparticle powder on the production of lignocellulose degrading enzymes in the fungus trametes versicolor.white rot fungi are one of the key group of microorganisms that help to enrich the soil via degradation of wood. in the current communication, influence of iron and copper nanoparticles on the production of lignocellulolytic enzymes by trametes versicolor have been investigated. the production of enzymes in the presence of the two nanoparticles was compared to that of ferrous and cupric ions respectively. results show that both the tested nanoparticles alter the production profile of the lignoce ...201020185234
degradation of phenols in olive oil mill wastewater by biological, enzymatic, and photo-fenton oxidation.olive oil mill wastewater (oomw) environmental impacts minimization have been attempted by developing more effective processes, but no chemical or biological treatments were found to be totally effective to mitigate their impact on receiving systems. this work is the first that reports simultaneously the efficiency of three different approaches: biological treatment by two fungal species (trametes versicolor or pleurotus sajor caju), enzymatic treatment by laccase, and chemical treatment by phot ...201019841956
white-rot fungus-mediated degradation of the analgesic ketoprofen and identification of intermediates by hplc-dad-ms and nmr.ketoprofen is a nonsteroidal anti-inflammatory drug that has been detected in the environment in the range of ng l(-1)-microg l(-1) due to its low degradability in some wastewater treatment plants. in this study, the use of the white-rot fungus trametes versicolor to effectively degrade ketoprofen in a defined liquid medium was assessed. the fungus eliminated ketoprofen to nondetectable levels in 24h when it was added at 10mgl(-1) whereas at low concentration of 40microgl(-1) it was almost compl ...201019913277
docking simulation and competitive experiments validate the interaction between the 2,5-xylidine inhibitor and rigidoporus lignosus laccase.laccases are polyphenol oxidases which oxidize a broad range of reducing substrates, preferably phenolic compounds, and their use in biotechnological applications is increasing. recently, the first x-ray structure of active laccase from white rot fungus rigidoporus lignosus has been reported containing a full complement of copper ions. comparison among selected fungal laccases of known 3d structure has shown that the rigidoporus lignosus laccase has a very high similarity with the trametes versi ...201019916571
mathematical model for trametes versicolor growth in submerged cultivation.trametes versicolor is a white-rot fungus known as a producer of extracellular enzymes such as laccase, manganese-peroxidase, and lignin-peroxidase. the production of these enzymes requires detailed knowledge of the growth characteristics and physiology of the fungus. submerged cultivations of t. versicolor on glucose, fructose, and sucrose as sole carbon sources were performed in shake flasks. sucrose hydrolysis catalyzed by the whole cells of t. versicolor was considered as one-step enzymatic ...201019949814
biodegradation of the analgesic naproxen by trametes versicolor and identification of intermediates using hplc-dad-ms and nmr.the white-rot fungus trametes vesicolor degraded naproxen (10 mg l(-1)) in a liquid medium to non-detectable levels after 6h. when naproxen was added in the range of concentrations typically found in the environment (55 microg l(-1)), it was almost completely degraded (95%) after 5h. in vitro degradation experiments with purified laccase and purified laccase plus mediator 1-hydroxybenzotriazol showed slight and almost complete naproxen degradation, respectively. a noticeable inhibition on naprox ...201020004093
role for toll-like receptor 4 in tnf-alpha secretion by murine macrophages in response to polysaccharide krestin, a trametes versicolor mushroom extract.woody fungi and yeast preparations show promise in cancer treatment by activating anti-tumor immune responses. macrophages (j774a.1) were treated with psk, reishi extract, scleroglucan or vehicle control. pre-incubation with tlr4 blocking antibody inhibited tnf-alpha secretion by both j774a.1 cells and primary splenocytes but had inconclusive effect on scleroglucan-induced secretion of tnf-alpha. psk induced tnf-alpha and il-6 secretion by wild type but not by tlr4-deficient peritoneal macrophag ...201020550956
application of laccase-mediator system (lms) for the degradation of organophosphorus compounds.degradation of organophosphorus compounds was achieved in the presence of purified fungal laccase from trametes versicolor and a small molecular weight redox mediator (abts). this laccase-mediator system (lms) catalyzed degradation of vx, phx and vr while had no apparent effect on cvx, ecothiophate or demeton. inhibition of abts oxidation was shown with vx, phx, vr and cvx. results with cvx suggest either no degradation subsequent to interaction with the laccase active site or the formation of a ...201020149786
regulatory properties of polysaccharopeptide derived from coriolus versicolor and its combined effect with ciclosporin on the homeostasis of human lymphocytes.lymphocyte homoeostasis is essential in inflammatory and autoimmune diseases. in search of natural fungal metabolites with effects on lymphocyte homoeostasis, we recently reported that polysaccharopeptide (psp) from coriolus versicolor exhibited ciclosporin-like activity in controlling aberrant lymphocyte activation. this object of this study was to investigate its effect on lymphocyte homoeostasis. this was done by investigating the mechanistic actions of psp in relation to ciclosporin by perfo ...201020663037
optimization of removal conditions of copper ions from aqueous solutions by trametes versicolor.a multi-step response surface methodology was successfully applied to optimize the biosorption conditions for the maximum removal of cu(ii) ions from aqueous solutions using trametes versicolor fungi as a biosorbent. in the first step, the most effective medium factors, which are ph, temperature and initial cu(ii) concentration, on biosorption of cu(ii), were determined through plackett-burman design. then steepest accent followed by central composite design steps were utilized to evaluate the o ...201020156677
degradation of naproxen and carbamazepine in spiked sludge by slurry and solid-phase trametes versicolor systems.growth and activity of the white-rot fungus trametes versicolor on sewage sludge were assessed in bioslurry and solid-phase systems. bioslurry cultures with different loads of sludge (10%, 25% and 38%, w/v) were performed. a lag phase of at least 2 d appeared in the 25 and 38%-cultures, however, the total fungal biomass was higher for the latter and lower for the 10%-culture after 30 d, as revealed by ergosterol determination. detectable laccase activity levels were found in the 10 and 25%-cultu ...201020031398
comparison of emulsion and vibration nozzle methods for microencapsulation of laccase and glucose oxidase by interfacial reticulation of poly(ethyleneimine).microcapsules for enzyme immobilization were successfully fabricated via interfacial cross-linking of poly(ethyleneimine) (pei). a method based on laminar jet break-up technique using a commercial instrument developed to produce alginate beads is reported for the first time for production of pei microcapsules. the diameter, wall thickness and pore size of membranes were obtained from confocal laser scanning microscopy by labelling pei and proteins. the composition of membranes was analysed by el ...201020716009
immunomodulatory effects of polysaccharopeptide (psp) in human pbmc through regulation of traf6/tlr immunosignal-transduction pathways.polysaccharopeptide (psp) was extracted from coriolus versicolor, and has been proved to be a valuable adjuvant for the combination with chemotherapy or radiotherapy in the treatment of various cancers. objective: to understand the mechanism of psp on immunomodulation, we examined gene expression and cytokine secretion associated with immunosignal-transduction signaling in human peripheral blood mononuclear cells (pbmcs).201020131955
effect of nitrogen sources and vitamins on ligninolytic enzyme production by some white-rot fungi. dye decolorization by selected culture filtrates.the effect of amino acids, complex nitrogen sources and vitamin addition on trametes trogii, trametes villosa and coriolus versicolor var. antarcticus ligninolytic enzyme production, was evaluated. dye decolorization by their culture filtrates was compared. glutamic acid followed by peptone, were the best n sources for laccase and manganese peroxidase production. the three fungi produced two laccase isoenzymes (molecular weights from 38 up to 150 kda); their pattern of production was not affecte ...201020153961
laccase-based biosensor for the determination of polyphenol index in wine.in this work we have developed and characterized the use of laccases from trametes versicolor (tvl) and trametes hirsuta (thl) as biocatalytic components of electrochemical biosensors for the determination of polyphenol index in wines. polyazetidine prepolimer (pap) was used as immobilizing agent, multi-walled and single-walled carbon nanotubes screen-printed electrodes as sensors (mwcnts-spe and swcnts-spe) and gallic acid as standard substrate. the amperometric measurements were carried out by ...201020188914
fungi immobilization for landfill leachate treatment.this paper investigated treatment of landfill leachate collected from nonthaburi landfill site, thailand, by using immobilized white rot fungi, namely, trametes versicolor bcc 8725 and flavodon flavus bcc 17421. effects of ph and co-substrates were investigated at different contact times. three types of co-substrates as carbon source used in this study are glucose, corn starch and cassava. treatment efficiency was evaluated based on color, bod, and cod removal. initial bod and cod were found to ...201020861536
naproxen degradation test to monitor trametes versicolor activity in solid-state bioremediation processes.the white-rot fungus trametes versicolor has been studied as a potential agent for the removal of environmental pollutants. for long-time solid-phase bioremediation systems a test is required to monitor the metabolic status of t. versicolor and its degradation capability at different stages. a biodegradation test based on the percentage of degradation of a spiked model pharmaceutical (anti-inflammatory naproxen) in 24 h (nd24) is proposed to monitor the removal of pharmaceuticals and personal ca ...201020347219
changes in oxidative enzyme activity during interspecific mycelial interactions involving the white-rot fungus trametes versicolor.interspecific fungal antagonism leads to biochemical changes in competing mycelia, including up-regulation of oxidative enzymes. laccase, manganese peroxidase (mnp), manganese-repressed peroxidase (mrp) and lignin peroxidase (lip) gene expression and enzyme activity were compared during agar interactions between trametes versicolor and five other wood decay fungi resulting in a range of interaction outcomes from deadlock to replacement of one fungus by another. increased laccase and mn-oxidising ...201020371297
construction and comparison of trametes versicolor laccase biosensors capable of detecting xenobiotics.amperometric biosensors using laccase from trametes versicolor as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-d). laccase enzyme was immobilized by gelatin and glutaraldehyde onto a clark oxygen probe and screen printed electrodes (spes). amperometric and chronoamperometric measurements were carried out with the biosensors. first, the effect of laccase activity on the biosensor performances was investigated for both biosensors, and then optimum ph and temperature and al ...201020380615
degradation of triclosan by an integrated nano-bio redox process.in this study, a sequential reduction-oxidation method was developed for complete degradation of triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether, tcs) in aqueous solution. rapid reductive dechlorination of tcs was achieved with palladized zero-valent iron nanoparticles (pd/nfe), under anaerobic conditions, with generation of 2-phenoxyphenol as the sole dechlorination product. sequentially, 2-phenoxyphenol was transformed into a non-toxic polymer using laccase (ec:1.10.3.2) derived from tram ...201020381343
reversible immobilization of laccase to poly(4-vinylpyridine) grafted and cu(ii) chelated magnetic beads: biodegradation of reactive dyes.poly(4-vinyl pyridine), poly(vp), as a novel metal-chelating fibrous polymer was grafted on the magnetic beads. poly(4-vp) grafted and/or cu(ii) ions chelated magnetic beads were used for reversible immobilization of trametes versicolor laccase, and the amounts of immobilized laccase on the beads were determined as 36.8 and 56.4 mg/g beads, respectively. the adsorption of laccase on both modified magnetic beads appeared to follow the langmuir isotherm model. the degradation of textile dyes with ...201020388589
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