Publications

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matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of fungal rhinosinusitis pathogens.filamentous fungi are among the most important pathogens, causing fungal rhinosinusitis (frs). current laboratory diagnosis of frs pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time consuming and expertise dependent. although matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms has been employed to identify various fungi, its efficacy in the identification of frs fungi is less clear.201728100314
a novel zn2-cys6 transcription factor atrr plays a key role in an azole resistance mechanism of aspergillus fumigatus by co-regulating cyp51a and cdr1b expressions.successful treatment of aspergillosis caused by aspergillus fumigatus is threatened by an increasing incidence of drug resistance. this situation is further complicated by the finding that strains resistant to azoles, the major antifungal drugs for aspergillosis, have been widely disseminated across the globe. to elucidate mechanisms underlying azole resistance, we identified a novel transcription factor that is required for normal azole resistance in aspergillus fungi including a. fumigatus, as ...201728052140
self-excising cre/mutant lox marker recycling system for multiple gene integrations and consecutive gene deletions in aspergillus oryzae.in this study, we developed a self-excising cre/loxp-mediated marker recycling system with mutated lox sequences to introduce a number of biosynthetic genes into aspergillus oryzae. to construct the self-excising marker cassette, both the selectable marker, the aspergillus nidulans adea gene, and the cre recombinase gene (cre), conditionally expressed by the xylanase-encoding gene promoter, were designed to be located between the mutant lox sequences, lox66 and lox71. however, construction of th ...201728011085
analysis of the ionic interaction between the hydrophobin roda and two cutinases of aspergillus nidulans obtained via an aspergillus oryzae expression system.hydrophobins are amphipathic secretory proteins with eight conserved cysteine residues and are ubiquitous among filamentous fungi. in the fungus aspergillus oryzae, the hydrophobin rola and the polyesterase cutl1 are co-expressed when the sole available carbon source is the biodegradable polyester polybutylene succinate-co-adipate (pbsa). rola promotes the degradation of pbsa by attaching to the particle surface, changing its structure and interacting with cutl1 to concentrate cutl1 on the pbsa ...201727917435
evaluating the effects of microwave-assisted hydrodistillation on antifungal and radical scavenging activities of oliveria decumbens and chaerophyllum macropodum essential oils.in this study, radical scavenging and antifungal activities of chaerophyllum macropodum and oliveria decumbens essential oils (eos) extracted with microwave-assisted hydrodistillation (mahd) were evaluated in comparison with the same eos extracted by conventional hydrodistillation (hd). the final eo yields that were obtained using hd (after 150 min) and mahd (after 45 min) were 1.72 and 1.67% for c. macropodum and 8.10 and 7.91% for o. decumbens, respectively. there were no significant differenc ...201728371590
mixed colonies of aspergillus niger and aspergillus oryzae cooperatively degrading wheat bran.in both natural and man-made environments, microorganisms live in mixed populations, while in laboratory conditions monocultures are mainly used. microbial interactions are often described as antagonistic, but can also be neutral or cooperative, and are generally associated with a metabolic change of each partner and cause a change in the pattern of produced bioactive molecules. a. niger and a. oryzae are two filamentous fungi widely used in industry to produce various enzymes (e.g. pectinases, ...201728232095
glucoamylase: a current allergen in the baking industry.over a 10 year period a decline in the rate of sensitizations to α-amylase (aspergillus oryzae) was observed in bakers investigated for allergic obstructive airway disease. at the same time, glucoamylase (aspergillus niger) was identified as the currently the most relevant allergen in sensitizations to enzymes in the baking industry. the aim of the present study was to investigate whether, over a period of 10 years and in the case of new-onset disease, there had been any change in sensitization ...201726120537
effect of magnesium acetate on the antimold activity of lactobacillus.the antimold activity of lactic acid bacteria (lab) is used in food biopreservation. the aim of this study was to evaluate the effect of magnesium acetate added to de man rogosa sharpe (mrs) medium on the antimold activity of three lab strains ( lactobacillus plantarum , lactobacillus brevis , and lactobacillus fermentum ) against molds contaminating food ( aspergillus oryzae , aspergillus niger , penicillium chrysogenum , fusarium avenaceum , and rhizopus arrhizus ) and their ability to produce ...201728221876
identification of a novel penicillium chrysogenum rhamnogalacturonan rhamnohydrolase and the first report of a rhamnogalacturonan rhamnohydrolase gene.rhamnogalacturonan (rg) i is one of the main components of pectins in the plant cell wall. we recently reported two rg i-degrading enzymes, endo-rg and exo-rg lyases, secreted by penicillium chrysogenum 31b. here, our aims were to purify a rg rhamnohydrolase (pcrgrh78a) from the culture filtrate of this strain and to characterize this enzyme. on the basis of the internal amino acid sequences, the encoding gene, pcrgrh78a, was cloned and overexpressed in aspergillus oryzae. the deduced amino acid ...201728110667
quantitative evaluation of haze formation of koji and progression of internal haze by drying of koji during koji making.the construction of an experimental system that can mimic koji making in the manufacturing setting of a sake brewery is initially required for the quantitative evaluation of mycelia grown on/in koji pellets (haze formation). koji making with rice was investigated with a solid-state fermentation (ssf) system using a non-airflow box (nab), which produced uniform conditions in the culture substrate with high reproducibility and allowed for the control of favorable conditions in the substrate during ...201728373030
cell wall α-1,3-glucan prevents α-amylase adsorption onto fungal cell in submerged culture of aspergillus oryzae.we have previously reported that α-amylase (taka-amylase a, taa) activity disappears in the later stage of submerged aspergillus oryzae culture as a result of taa adsorption onto the cell wall. chitin, one of the major components of the cell wall, was identified as a potential factor that facilitates taa adsorption. however, taa adsorption only occurred in the later stage of cultivation, although chitin was assumed to be sufficiently abundant in the cell wall regardless of the submerged culture ...201728356219
carbon and nitrogen depletion-induced nucleophagy and selective autophagic sequestration of a whole nucleus in multinucleate cells of the filamentous fungus aspergillus oryzae.autophagy is a conserved cellular degradation process in eukaryotes, in which cytoplasmic components and organelles are digested in vacuoles/lysosomes. recently, autophagic degradation of nuclear materials, termed "nucleophagy", has been reported. in the multinucleate filamentous fungus aspergillus oryzae, a whole nucleus is degraded by nucleophagy after prolonged culture. while developing an h2b-egfp processing assay for the evaluation of nucleophagy in a. oryzae, we found that nucleophagy is e ...201728331162
synthesis of lactulose in batch and repeated-batch operation with immobilized β-galactosidase in different agarose functionalized supports.lactulose synthesis was done in repeated-batch mode with aspergillus oryzae β-galactosidase immobilized in glyoxyl-agarose (ga-βg), amino-glyoxyl-agarose (am-ga-βg) and chelate-glyoxyl-agarose (che-ga-βg), at fructose/lactose molar ratios of 4, 12 and 20. highest yields of lactulose in batch were obtained with che-ga-βg (0.21, 0.29 and 0.32g·g(-1)) for 4, 12 and 20 fructose/lactose molar ratios respectively; when operating in 10 repeated batches highest product to biocatalyst mass ratios were ob ...201728160659
ovicidal effect of chitinase and protease enzymes produced by soil fungi on the camel tick hyalomma dromedarii eggs (acari:ixodidae).the aim of this study was to evaluate the effect of chitinase and protease enzymes produced by environmentally safe soil fungi; aspergillus sp. nrc 4/5h; mucor sp. nrc 5; trichoderma sp. nrc 4/56; aspergillus sojae; mucor ranosisinus; aspergillus oryzae on embryo development of the camel tick hyalomma dromedarii eggs. the experiment was carried out on the 7 day aged eggs. concentrations of the chitinase and protease crude enzymes [1:1, 1:2, 1:3, 1:4 and 1:5 (v/v)] were prepared from six stock so ...201728316424
high-efficiency extracellular release of free fatty acids from aspergillus oryzae using non-ionic surfactants.free fatty acids (ffas) are useful for generating biofuel compounds and functional lipids. microbes are increasingly exploited to produce ffas via metabolic engineering. however, in many microorganisms, ffas accumulate in the cytosol, and disrupting cells to extract them is energy intensive. thus, a simple cost-effective extraction technique must be developed to remove this drawback. we found that ffas were released from cells of the filamentous fungus aspergillus oryzae with high efficiency whe ...201728300661
biosorption of copper (ii) from aqueous solutions by sclerotiogenic aspergillus oryzae g15.this study aimed at investigating the biosorption of the copper(ii) from aqueous solutions by sclerotiogenic aspergillus oryzae g15. potentiometric titration analysis results indicated that carboxyl group was mainly responsible for cu(ii) adsorption. sclerotia were superior biosorbent than mycelia, which could be ascribed to the higher amount of carboxyl sites exposed after differentiation. langmuir isotherm model fitted well the absorption process of mycelia and sclerotia with the maximum theor ...201728276318
analysis of microbial diversity in shenqu with different fermentation times by pcr-dgge.shenqu is a fermented product that is widely used in traditional chinese medicine (tcm) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. the aim of this study was to investigate microbial diversity in shenqu using different fermentation time periods. dgge (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles indicated that a strain of pediococcus acidilactici (band 9) is the predominant bacteria during fermentation and tha ...201728256389
functional expression of a novel α-amylase from antarctic psychrotolerant fungus for baking industry and its magnetic immobilization.α-amylase plays a pivotal role in a broad range of industrial processes. to meet increasing demands of biocatalytic tasks, considerable efforts have been made to isolate enzymes produced by extremophiles. however, the relevant data of α-amylases from cold-adapted fungi are still insufficient. in addition, bread quality presents a particular interest due to its high consummation. thus developing amylases to improve textural properties could combine health benefits with good sensory properties. fu ...201728245836
a comparative secretome analysis of industrial aspergillus oryzae and its spontaneous mutant zjgs-lz-21.aspergillus oryzae koji plays a crucial role in fermented food products due to the hydrolytic activities of secreted enzymes. in the present study, we performed a comparative secretome analysis of the industrial strain of aspergillus oryzae 3.042 and its spontaneous mutantzjgs-lz-21. one hundred and fifty two (152) differential protein spots were excised (p<0.05), and 25 proteins were identified. of the identified proteins, 91.3% belonged to hydrolytic enzymes acting on carbohydrates or proteins ...201728237882
immobilization of laccase from aspergillus oryzae on graphene nanosheets.laccase enzymes of aspergillus oryzae were immobilized on graphene nanosheets by physical adsorption and covalent bonding. morphological features of the graphene sheets were characterized via microscopy techniques. the immobilization by adsorption was carried out through contact between graphene and solution of laccase enzyme dissolved in deionized water. the adsorption process followed a freundlich model, showing no tendency to saturation within the range of values used. the process of immobili ...201728237573
effects of nonionic surfactants on pellet formation and the production of β-fructofuranosidases from aspergillus oryzae kb.aspergillus oryzae kb produces two β-fructofuranosidases (f1 and f2). f1 has high transferring activity and produces fructooligosaccharides from sucrose. mycelial growth pellets were altered by the addition of tween 20, 40 and 80 (hlb=16.7, 15.6 and 15.0, respectively) in liquid medium cultures to form small spherical pellets. the particle size of the pellets decreased with the hlb value, which corresponds to an increase in surfactant hydrophobicity. selective f1 production and pellet size were ...201728159248
aos28d, a proline-xaa carboxypeptidase secreted by aspergillus oryzae.prolyl peptidases of the merops s28 family are of particular interest because they are key enzymes in the digestion of proline-rich peptides. a blast analysis of the aspergillus oryzae genome revealed sequences coding for four proteases of the s28 family. three of these proteases, aos28a, aos28b, and aos28c, were previously characterized as acidic prolyl endopeptidases. the fourth protease, aos28d, showed high sequence divergence with other s28 proteases and belongs to a phylogenetically distinc ...201728229206
immobilization of aspergillus oryzae β-galactosidase in an agarose matrix functionalized by four different methods and application to the synthesis of lactulose.aspergillus oryzae β-galactosidase was immobilized in monofunctional glyoxyl-agarose and heterofunctional supports (amino-glyoxyl, carboxy-glyoxyl and chelate-glyoxyl agarose), for obtaining highly active and stable catalysts for lactulose synthesis. specific activities of the amino-glyoxyl agarose, carboxy-glyoxyl agarose and chelate-glyoxyl agarose derivatives were 3676, 430 and 454iu/g biocatalyst with half-life values at 50°c of 247, 100 and 100h respectively. specific activities of 3490, 25 ...201728214445
tolerance and stress response of sclerotiogenic aspergillus oryzae g15 to copper and lead.aspergillus oryzae g15 was cultured on czapek yeast extract agar medium containing different concentrations of copper and lead to investigate the mechanisms sustaining metal tolerance. the effects of heavy metals on biomass, metal accumulation, metallothionein (mt), malondialdehyde (mda), superoxide dismutase (sod), catalase (cat), and peroxidase (pod) were evaluated. cu and pb treatment remarkably delayed sclerotial maturation and inhibited mycelial growth, indicating the toxic effects of the m ...201728132138
biochemical characterization of thermostable β-1,4-mannanase belonging to the glycoside hydrolase family 134 from aspergillus oryzae.a β-1,4-mannanase, termed aoman134a, that belongs to the gh 134 family was identified in the filamentous fungus aspergillus oryzae. recombinant aoman134a was expressed in pichia pastoris, and the purified enzyme produced mannobiose, mannotriose, mannotetraose, and mannopentaose from galactose-free β-mannan, with mannotriose being the predominant reaction product. the catalytic efficiency (k cat/k m ) of aoman134a was 6.8-fold higher toward galactomannan from locust bean gum, than toward galactom ...201728105485
positive and negative aspects of green coffee consumption - antioxidant activity versus mycotoxins.the quality of coffee depends not only on the contents of healthy compounds but also on its contamination with microorganisms that can produce mycotoxins during development, harvesting, preparation, transport and storage.201728195330
reaction kinetics and galactooligosaccharide product profiles of the β-galactosidases from bacillus circulans, kluyveromyces lactis and aspergillus oryzae.β-galactosidase enzymes are used in the dairy industry to convert lactose into galactooligosaccharides (gos) that are added to infant formula to mimic the molecular sizes and prebiotic functions of human milk oligosaccharides. here we report a detailed analysis of the clearly different gos profiles of the commercial β-galactosidases from bacillus circulans, kluyveromyces lactis and aspergillus oryzae. also the gos yields of these enzymes differed, varying from 48.3% (b. circulans) to 34.9% (k. l ...201728193420
fed-batch operation for the synthesis of lactulose with β-galactosidase of aspergillus oryzae.fed-batch synthesis of lactulose from lactose and fructose with aspergillus oryzae β-galactosidase was evaluated, obtaining a concentration of 40.4g·l(-1), which is 20% higher than obtained in batch, while the concentration of transgalactosylated oligosaccharides (tos) was reduced by 98%. therefore, selectivity of lactulose synthesis can be significantly higher by operating in fed-batch mode. the enzyme-limiting substrate mass ratio (e/s) is a critical variable in fed-batch operation. higher val ...201728162924
cellular responses to the expression of unstable secretory proteins in the filamentous fungus aspergillus oryzae.filamentous fungi are often used as cell factories for recombinant protein production because of their ability to secrete large quantities of hydrolytic enzymes. however, even using strong transcriptional promoters, yields of nonfungal proteins are generally much lower than those of fungal proteins. recent analyses revealed that expression of certain nonfungal secretory proteins induced the unfolded protein response (upr), suggesting that they are recognized as proteins with folding defects in f ...201728064367
deletion of admb gene encoding a fungal adam affects cell wall construction in aspergillus oryzae.mammals possess a unique signaling system based on the proteolytic mechanism of a disintegrin and metalloproteinases (adams) on the cell surface. we found two genes encoding adams in aspergillus oryzae and named them adma and admb. we produced adma and admb deletion strains to elucidate their biological function and clarify whether fungal adams play a similar role as in mammals. the ∆adma∆admb and ∆admb strains were sensitive to cell wall-perturbing agents, congo red, and calcofluor white. moreo ...201728058997
learning from oligosaccharide soaks of crystals of an aa13 lytic polysaccharide monooxygenase: crystal packing, ligand binding and active-site disorder.lytic polysaccharide monooxygenases (lpmos) are a class of copper-dependent enzymes discovered within the last ten years. they oxidatively cleave polysaccharides (chitin, lignocellulose, hemicellulose and starch-derived), presumably making recalcitrant substrates accessible to glycoside hydrolases. recently, the first crystal structure of an lpmo-substrate complex was reported, giving insights into the interaction of lpmos with β-linked substrates (frandsen et al., 2016). the lpmos acting on α-l ...201728045386
mutational analysis of kex2 recognition sites and a disulfide bond in tannase from aspergillus oryzae.aspergillus oryzae tannase (aotana), which contains two kex2 recognition sites at positions arg311 and arg316, consists of two subunits that are generated by the cleavage of tannase gene product by the kex2 protease. based on the crystal structure of feruloyl esterase from aspergillus oryzae (aofaeb), which has been classified as a member of the fungal tannase family, the catalytic triad residues of aotana are predicted to be ser195, asp455, and his501, with the serine and histidine residues bro ...201727919681
microbial diversity during fermentation of sweet paste, a chinese traditional seasoning, using pcr-denaturing gradient gel electrophoresis.the aim of this study was to elucidate the changes in the microbial community and biochemical properties of a traditional sweet paste during fermentation. pcr-denaturing gradient gel electrophoresis (dgge) analysis showed that aspergillus oryzae was the predominant species in the koji and the majority of the fungi isolated belonged to 2 zygosaccharomyces species in the mash. the bacterial dgge profiles revealed the presence of bacillus subtilis during fermentation, and lactobacillus acidipiscis, ...201728081358
enzymatic production of 5'-inosinic acid by amp deaminase from a newly isolated aspergillus oryzae.5'-adenylic acid deaminase (amp deaminase), an important enzyme for the food industry, can catalyze the irreversible hydrolysis of adenosine monophosphate (amp) to inosine monophosphate (imp) and ammonia. in this study, a new strain was screened that efficiently produces 3191.6u/g of amp deaminase at 32°c. after purification, the optimal temperature and ph of the amp deaminase were found to be 40°c and 6.0, respectively, but it was partially inhibited by fe(3+), cu(2+), al(3+), and zn(2+). with ...201727596420
fermented brown rice extract stimulates bdnf gene transcription in c6 glioma cells: possible connection with ho-1 expression.fermented brown rice with aspergillus oryzae, designated as fbra, is known to be commercially available dietary fiber-rich food, which is appreciated as prebiotics to improve intestinal microflora, and also shown to contain various biologically active substances including polyphenolic compounds. on the other hand, polyphenolic compounds have been suggested to stimulate the expression of brain-derived neurotrophic factor (bdnf) gene in connection with the expression of heme oxidase-1 (ho-1) gene ...201727560964
detailed analysis of targeted gene mutations caused by the platinum-fungal talens in aspergillus oryzae rib40 strain and a ligd disruptant.transcription activator-like effector nucleases (talens), which can generate dna double-strand breaks at specific sites in the desired genome locus, have been used in many organisms as a tool for genome editing. in aspergilli, including aspergillus oryzae, however, the use of talens has not been validated. in this study, we performed genome editing of a. oryzae wild-type strain via error of nonhomologous end-joining (nhej) repair by transient expression of high-efficiency platinum-fungal talens ...201727780671
optimisation of nutritional requirements for dopamine synthesis by calcium alginate-entrapped mutant strain of aspergillus oryzae ems-6.the optimisation of nutritional requirements for dopamine (da) synthesis by calcium alginate-entrapped mutant variant of aspergillus oryzae ems-6 using submerged fermentation technique was investigated. a total of 13 strains were isolated from soil. isolate i-2 was selected as a better producer of da and improved by exposing with ethyl methylsulphonate (ems). ems-6 was selected as it exhibited 43 μg/ml da activity. the mutant variable was further treated with low levels of l-cysteine hcl to make ...201727686520
effects of addition of aspergillus oryzae culture and 2-hydroxyl-4-(methylthio) butanoic acid on milk performance and rumen fermentation of dairy cows.to investigate effects of aspergillus oryzae culture (aoc) and 2-hydroxy-4-(methylthio) butanoic acid (hmb) on milk performance and rumen fermentation of dairy cows. sixty-four multiparous chinese holstein cows were randomly allocated into four experimental diets: (i) control diet; (ii) aoc diet: 5 g aoc/day per head; (iii) hmb diet: 25 g hmb/day; and (iv) ah diet: 5 g aoc plus 25 g hmb/day. added hmb tended to increase the yield of milk protein (p = 0.06) and 3.5% fat-corrected milk (p = 0.08) ...201727506446
simultaneous conversion of free fatty acids and triglycerides to biodiesel by immobilized aspergillus oryzae expressing fusarium heterosporum lipase.the presence of high levels of free fatty acids (ffa) in oil is a barrier to one-step biodiesel production. undesirable soaps are formed during conventional chemical methods, and enzyme deactivation occurs when enzymatic methods are used. this work investigates an efficient technique to simultaneously convert a mixture of free fatty acids and triglycerides (tag). a partial soybean hydrolysate containing 73.04% free fatty acids and 24.81% triglycerides was used as a substrate for the enzymatic pr ...201727860394
enhanced production of fructosyltransferase in aspergillus oryzae by genome shuffling.to breed aspergillus oryzae strains with high fructosyltransferase (ftase) activity using intraspecific protoplast fusion via genome-shuffling.201727853895
aoatg26, a putative sterol glucosyltransferase, is required for autophagic degradation of peroxisomes, mitochondria, and nuclei in the filamentous fungus aspergillus oryzae.autophagy is a conserved process in eukaryotic cells for degradation of cellular proteins and organelles. in filamentous fungi, autophagic degradation of organelles such as peroxisomes, mitochondria, and nuclei occurs in basal cells after the prolonged culture, but its mechanism is not well understood. here, we functionally analyzed the filamentous fungus aspergillus oryzae aoatg26, an ortholog of the sterol glucosyltransferase ppatg26 involved in pexophagy in the yeast pichia pastoris. deletion ...201727696999
production and biochemical characterization of an alkaline protease from aspergillus oryzae ch93.in this study, aspergillus oryzae ch93 was isolated from soil sample and examined using molecular analysis. following culture of a. oryzae ch93 under optimal enzyme production, a 47.5kda extracellular protease was purified using ammonium sulfate precipitation and q-sepharose chromatography. the optimal ph 8 and temperature of 50°c obtained for the isolated protease. sodium dodecyl sulfate (sds), cetyltrimethyl ammonium bromide (ctab), h2o2 decreased activity, while triton x-100 and phenylmethane ...201727293035
comparative thermal inactivation analysis of aspergillus oryzae and thiellavia terrestris cutinase: role of glycosylation.cutinase thermostability is important so that the enzymes can function above the glass transition of what are often rigid polymer substrates. a detailed thermal inactivation analysis was performed for two well-characterized cutinases, aspergillus oryzae cutinase (aoc) and thiellavia terrestris cutinase (ttc). both aoc and ttc are prone to thermal aggregation upon unfolding at high temperature, which was found to be a major reason for irreversible loss of enzyme activity. our study demonstrates t ...201727425027
modeling and verification of process parameters for the production of tannase by aspergillus oryzae under submerged fermentation using agro-wastes.tannase production by aspergillus oryzae using various agro-wastes as substrates by submerged fermentation was studied in this research. the microbe was isolated from degrading corn kernel obtained from the corn fields at tiruchengode, india. the microbial identification was done using 18s rrna gene analysis. the agro-wastes chosen for the study were pomegranate rind, cassia auriculata flower, black gram husk, and tea dust. the process parameters chosen for optimization study were substrate conc ...201726498327
characterizations of the submerged fermentation of aspergillus oryzae using a fullzone impeller in a stirred tank bioreactor.a fullzone (fz) impeller was used in the first study of the characteristics involved in the fermentation of aspergillus oryzae. both the experimental and simulation results of this study revealed novel findings into the positive relationship between the global-axial mixing patterns of a fz impeller and fermentation efficiency. the mixing results when using the fz impeller compared with a double rushton turbine (drt) impeller indicated that the culture mixed by the fz resulted in a more homogeneo ...201727475924
homology modeling and molecular dynamics study on schwanniomyces occidentalis alpha-amylase.with consumers growing increasingly aware of environmental issues, industries find enzymes as a reasonable alternative over physical conditions and chemical catalysts. amylases are important hydrolase enzymes, which have been widely used in variety of industrial process such as pharmaceutical, food, and fermentation industries. among amylases α-amylase is in maximum demand due to its wide range of applications. the homology modeling study on schwanniomyces occidentalis amylase (amy1, uniprot ide ...201726882245
harnessing biodiesel-producing microbes: from genetic engineering of lipase to metabolic engineering of fatty acid biosynthetic pathway.microbial production routes, notably whole-cell lipase-mediated biotransformation and fatty-acids-derived biosynthesis, offer new opportunities for synthesizing biodiesel. they compare favorably to immobilized lipase and chemically catalyzed processes. genetically modified whole-cell lipase-mediated in vitro route, together with in vivo and ex vivo microbial biosynthesis routes, constitutes emerging and rapidly developing research areas for effective production of biodiesel. this review presents ...201726526353
proteolysis in tempeh-type products obtained with rhizopus and aspergillus strains from grass pea (lathyrus sativus) seeds.tempeh is a food product obtained from legumes by means of solid-state fermentation with rhizopus sp. our previous research proved that mixed-culture inoculum may also be successfully applied. the objective of present research was to study the proteolytic activity of r. microsporus var. chinensis and a. oryzae during tempeh-type fermentation of grass pea seeds, and the effect of inoculum composition on the protein level and in vitro protein bioavailability in products.201728068010
direct alkylation of amines with primary and secondary alcohols through biocatalytic hydrogen borrowing.the reductive aminase from aspergillus oryzae (aspredam) was combined with a single alcohol dehydrogenase (either metagenomic adh-150, an adh from sphingobium yanoikuyae (syadh), or a variant of the adh from thermoanaerobacter ethanolicus (tesadh w110a)) in a redox-neutral cascade for the biocatalytic alkylation of amines using primary and secondary alcohols. aliphatic and aromatic secondary amines were obtained in up to 99 % conversion, as well as chiral amines directly from the racemic alcohol ...201728671344
synthesis of supermacroporous cryogel for bioreactors continuous starch hydrolysis.a bioreactor was built by means of immobilizing alpha-amylase from aspergillus oryzae by encapsulation, through cryopolymerization of acrylamide monomers for the continuous starch hydrolysis. the starch hydrolysis was evaluated regarding ph, the concentration of immobilized amylase on cryogel, the concentration of starch solution and temperature. the maximum value for starch hydrolysis was achieved at ph 5.0, concentration of immobilized enzyme 111.44mg amylase /gcriogel , concentration of starc ...201728777449
purification and characterization of a newly serine protease inhibitor from rhamnus frangula with potential for use as therapeutic drug.protease inhibitors from plants are well known to be potent inhibitors of the growth of bacteria, fungi, and even certain viruses which make them excellent candidates for use as the lead compounds for the development of novel antimicrobial agents for applications in medicine. in this study, rhamnus frangula was selected as a protease inhibitor source. the maximum recovery of the protease inhibitor against trypsin was recorded in the crude extract made in 0.1 m phosphate buffer (ph 7.0) and isola ...201728597160
identification and functional analysis of a new glyphosate resistance gene from a fungus cdna library.glyphosate is a widely used broad spectrum herbicide; however, this limits its use once crops are planted. if glyphosate-resistant crops are grown, glyphosate can be used for weed control in crops. while several glyphosate resistance genes are used in commercial glyphosate tolerant crops, there is interest in identifying additional genes for glyphosate tolerance. this research constructed a high-quality cdna library form the glyphosate-resistant fungus aspergillus oryzae rib40 to identify genes ...201728755696
immobilization of β-galactosidase on surface modified cobalt/multiwalled carbon nanotube nanocomposite improves enzyme stability and resistance to inhibitor.the present study aimed to work out a high yield procedure for immobilization of aspergillus oryzae β-galactosidase on polyaniline cobalt multiwalled carbon nanotubes nanocomposite (pani/co/mwcntnc) by physical adsorption and covalent attachment via glutaraldehyde. the binding was confirmed by scanning and transmission electron microscopy along with fourier transform-infrared spectroscopy. the immobilization yields obtained for adsorbed and cross-linked enzymes were 93% and 97%, respectively. th ...201728735002
a new and efficient approach for construction of uridine/uracil auxotrophic mutants in the filamentous fungus aspergillus oryzae using agrobacterium tumefaciens-mediated transformation.aspergillus oryzae is a filamentous fungus widely used in food industry and as a microbial cell factory for recombinant protein production. due to the inherent resistance of a. oryzae to common antifungal compounds, genetic transformation of this mold usually requires auxotrophic mutants. in this study, we show that agrobacterium tumefaciens-mediated transformation (atmt) method is very efficient for deletion of the pyrg gene in different aspergillus oryzae wild-type strains to generate uridine/ ...201728466303
comparison in antioxidant and antitumor activities of pine polyphenols and its seven biotransformation extracts by fungi.microbial transformation can strengthen the antioxidant and antitumor activities of polyphenols. polyphenols contents, antioxidant and antitumor activities of pine polyphenols and its biotransformation extracts by aspergillus niger, aspergillus oryzae, aspergillus carbonarius, aspergillus candidus, trichodermas viride, mucor wutungkiao and rhizopus sp were studied. significant differences were noted in antioxidant and antitumor activities. the highest antioxidant activities in trolox equivalent ...201728560092
improved production of kojic acid by mutagenesis of aspergillus flavus hak1 and aspergillus oryzae hak2 and their potential antioxidant activity.two wild-type (wt) aspergillus strains, a. flavus hak1 and a. oryzae hak2, were selected for kojic acid (ka) biosynthesis. malt extract sucrose culture medium (mes) was the best culture medium for maximum production of ka. the maximum production of ka has been estimated at ph 4 after 7 days of incubation at 30 °c. overproduction of ka was attained by mutagenesis of both a. flavus hak1 and a. oryzae hak2 through their exposer to different doses of gamma irradiation. the mutant strains (mt) a. fla ...201728794931
diversity, application, and synthetic biology of industrially important aspergillus fungi.the filamentous fungal genus aspergillus consists of over 340 officially recognized species. a handful of these aspergillus fungi are predominantly used for food fermentation and large-scale production of enzymes, organic acids, and bioactive compounds. these industrially important aspergilli primarily belong to the two major aspergillus sections, nigri and flavi. aspergillus oryzae (section flavi) is the most commonly used mold for the fermentation of soybeans, rice, grains, and potatoes. asper ...201728732553
enzymatic characterization of a novel xaa-pro aminopeptidase xpma from aspergillus oryzae expressed in escherichia coli.xaa-pro aminopeptidases are peptidases responsible for the cleavage of any amino acid n-terminally adjacent to a proline residue. we identified a gene encoding a putative xaa-pro aminopeptidase in the genome of the filamentous fungus aspergillus oryzae (genome database number: ao090701000720) and named this gene xpma. we produced its enzyme in a c-terminally his6-tag-fused form in an escherichia coli expression system and purified it. the purified recombinant xpma (rxpma) showed hydrolysis activ ...201728698148
structural features of aspergillus niger β-galactosidase define its activity against glycoside linkages.β-galactosidases are biotechnologically interesting enzymes that catalyze the hydrolysis or transgalactosylation of β-galactosides. among them, the aspergillus niger β-galactosidase (anβgal) belongs to the glycoside hydrolase family 35 (gh35) and is widely used in the industry due to its high hydrolytic activity which is used to degrade lactose. we present here its three-dimensional structure in complex with different oligosaccharides, to illustrate the structural determinants of the broad speci ...201728391618
comparison between the univariate and multivariate analysis on the partial characterization of the endoglucanase produced in the solid state fermentation by aspergillus oryzae atcc 10124.endoglucanase production by aspergillus oryzae atcc 10124 cultivated in rice husks or peanut shells was optimized by experimental design as a function of humidity, time and temperature. the optimum temperature for the endoglucanase activity was estimated by a univariate analysis (one factor at the time) as 50 °c (rice husks) and 60 °c (peanut shells), however, by a multivariate analysis (synergism of factors) it was determined a different temperature (56 °c) for endoglucanase from peanut shells. ...201728857660
development of a versatile and conventional technique for gene disruption in filamentous fungi based on crispr-cas9 technology.filamentous fungi represent an invaluable source of pharmaceutically active compounds. the development of versatile methods to genetically manipulate filamentous fungi is of great value for improving the low yields of bioactive metabolites and expanding chemical diversity. the crispr-cas9-based system has become a common platform for genome editing in a variety of organisms. however, recent application of this technology in filamentous fungi is limited to model strains, a versatile method for ef ...201728835711
development of a mouse model of eosinophilic chronic rhinosinusitis with nasal polyp by nasal instillation of an aspergillus protease and ovalbumin.one subtype of chronic rhinosinusitis with nasal polyps (crswnp) is characterized by the development of a t-helper type 2 (th2) response and eosinophilic infiltration. here, we aimed to establish an eosinophilic crswnp murine model, which would be essential to understand the underlying pathogenesis and establish a treatment strategy. c57bl/6 mice were challenged intranasally with a mixture of an aspergillus oryzae-derived protease (ap) and ovalbumin (ova) for 6, 8, or 12 consecutive weeks (12 mi ...201728828551
consumption of an acid protease derived from aspergillus oryzae causes bifidogenic effect in rats.a marked elevation in the abundance of bifidobacterium was found in the cecum of rats that were fed a high-fat diet supplemented with an amano protease preparation (derived from aspergillus oryzae). the protease preparation contains several digestive enzymes, including acid protease (acp), alkaline protease, and amylase. we hypothesized that the elevation in the abundance of bifidobacterium by amano protease preparation is associated with the digestive enzymes involved in the protease preparatio ...201728821318
fungal endocarditis due to aspergillus oryzae: the first case reported in the literature.infective endocarditis (ie) is a severe disease with high mortality and morbidity. prosthetic valve endocarditis is a life-threatening complication which can occur in less than 10% of patients with valve prosthesis. a fungal etiology of ie is rare and accounts for only 2-4% of all case of endocarditis, but is associated with a higher mortality and morbidity. herein is reported the first case of fungal endocarditis of aortic valve prosthesis due to aspergillus oryzae in a 67-year-old caucasian ma ...201728820551
biochemical characterization of aspergillus oryzae recombinant α-l-rhamnosidase expressed in pichia pastoris.an α-l-rhamnosidase-encoding gene from aspergillus oryzae, which belongs to the glycoside hydrolase family 78, was cloned and expressed in pichia pastoris. sds-page of the purified recombinant α-l-rhamnosidase protein revealed smeared bands with apparent molecular mass of 90-130 kda. after n-deglycosylation, the recombinant enzyme showed a molecular mass of 70 kda. the enzyme exhibited optimal activity at a ph of 5.0 and a temperature of 70 °c. specific activity of the enzyme was higher toward h ...201728800907
characteristics of korean alcoholic beverages produced by using rice nuruks containing aspergillus oryzae n159-1.herein, nuruks derived from non-glutinous and glutinous rice inoculated with aspergillus oryzae n159-1 (having high alpha-amylase and beta-glucosidase activities) were used to produce korean alcoholic beverages. the resultant beverages had enhanced fruity (ethyl caproate and isoamyl alcohol) and rose (2-phenethyl acetate and phenethyl alcohol) flavors and high taste scores.201728781547
combinatorial biosynthesis of (+)-daurichromenic acid and its halogenated analogue.daurichromenic acid is a meroterpenoid with various pharmacological activities that is biosynthesized from grifolic acid in rhododendron dauricum. heterologous expression of grifolic acid synthases from stachybotrys bisbyi and a daurichromenic acid synthase from r. dauricum in aspergillus oryzae mediated three-step combinatorial biosynthesis of (+)-daurichromenic acid through enantioselective 6-endo-trig cyclization. additional introduction of a halogenase from fusarium sp. into the strain resul ...201728541042
free and immobilized aspergillus oryzae sbs50 producing protease-resistant and thermostable phytase.optimization for enhanced phytase production by aspergillus oryzae sbs50 in submerged fermentation was investigated using taguchi design. in first step design, starch, beef extract, magnesium sulphate, ferrous sulphate and tween 80 were identified as significant factors affecting phytase production. these significant factors were further optimized at four different levels using a second taguchi design and were observed that 1% starch, 2% beef extact, 3% tween 80, 0.1% magnesium sulphate and 0.22 ...201728669072
comparative analysis of polyspecificity of the endogenous trna synthetase of different expression host towards photocrosslinking amino acids using an in silico approach.photo-induced covalent crosslinking has emerged as the powerful strategy for analyzing and characterizing the protein-protein interaction and mapping protein 3d conformations. in the last decades, a number of photocrosslinking amino acids have been reported but only a few have been efficiently utilized for photocrosslinking purposes. recently, incorporation of diazirine containing photoactivatable analogs such as photo-methionine, photo-leucine, photo-isoleucine and photo-lysine into target prot ...201728641210
expression and activity analysis of fructosyltransferase from aspergillus oryzae.the fructosyltransferase gene was isolated and cloned from aspergillus oryzae. the gene was 1368 bp, which encoded a protein of 455 amino acids. to analyze the activity of the expressed fructosyltransferase, the pet32a-fructosyltransferase recombined plasmid was transformed into escherichia coli bl21. the fructosyltransferase gene was successfully expressed by isopropyl-β-d-thiogalactoside (iptg) induction. the molecular weight of the expression protein was about 45 kda. the optimal conditions o ...201728639160
pathway and rate-limiting step of glyphosate degradation by aspergillus oryzae a-f02.aspergillus oryzae a-f02, a glyphosate-degrading fungus, was isolated from an aeration tank in a pesticide factory. the pathway and rate-limiting step of glyphosate (gp) degradation were investigated through metabolite analysis. gp, aminomethylphosphonic acid (ampa), and methylamine were detected in the fermentation liquid of a. oryzae a-f02, whereas sarcosine and glycine were not. the pathway of gp degradation in a. oryzae a-f02 was revealed: gp was first degraded into ampa, which was then degr ...201728636478
comparison of fungicidal properties of non-thermal plasma produced by corona discharge and dielectric barrier discharge.the inactivation of four micromycete species by action of non-thermal plasma was followed. two sources of plasma were compared, namely, positive corona discharge and dielectric barrier discharge. the corona discharge appeared as suitable for fungal spore inactivation in water suspension, whereas the barrier discharge inactivated spores on the surface of cultivation agar. cladosporium sphaerospermum was the most sensitive, being inactivated within 10 min of exposure to plasma, whereas aspergillus ...201728623536
design, synthesis, and biological evaluation of vanillin hydroxamic acid derivatives as novel peptide deformylase inhibitors.infectious disease is increasingly hampering human health, which challenge the discovery of new antibacterial target. peptide deformylase (pdf), a metalloenzyme responsible for catalyzing the remove of the n-formyl group from nascent proteins, was considered as an important target in antibacterial drug discovery.201728606047
isotope-coded derivatization based lc/esi-ms/ms methods using a pair of novel reagents for quantification of hydroxycinnamic acids and hydroxybenzoic acids in fermented brown rice product.hydroxycinnamic acids (hcas) and hydroxybenzoic acids (hbas) are antioxidant phytochemicals found in rice and effective for the prevention of human diseases including cancer. fbra, which is a functional food manufactured by fermenting brown rice and rice bran with aspergillus oryzae, has been demonstrated to have chemopreventive effects against carcinogenesis in various organs. in this study, we developed methods for the relative and absolute quantification of ferulic acid, sinapic acid, caffeic ...201728531830
dietary safety of a dual-enzyme preparation for animal feed: acute and subchronic oral toxicity and genotoxicity studies.animal feed is routinely supplemented with exogenous enzymes to improve nutrient utilization, such as proteases to enhance protein hydrolysis in vivo and xylanases to alleviate feed related anti-nutritional factors. the present studies were conducted to evaluate the potential oral toxicity and genotoxicity of a dual-enzyme preparation, vegpro(®) concentrate (vpr-c). acute oral toxicity studies were conducted in adult male and female sprague-dawley crl cd rats and chs swiss ico:ofi (iops caw) mic ...201728583667
purification and characterization of a protease-resistant phytase of aspergillus oryzae sbs50 whose properties make it exceptionally useful as a feed supplement.an extracellular phytase of aspergillus oryzae sbs50 was purified to homogeneity using ammonium sulphate precipitation, ion-exchange and gel filtration chromatography. purified phytase has a monomeric molecular mass of ∼80kda exhibiting its optimal activity at ph 5.0 and 50°c with a t 1/2 of 300min at 50°c. phytase of a. oryzae displayed broad substrate specificity with vmax and km values of 58.82μmol/ml/min and 1.14mm, respectively, for calcium phytate. purity and homogeneity of the phytase was ...201728527994
impacts of amino acid substitutions in fungal cytochrome p450 monooxygenase (cyp57b3) on the effective production of 3΄-hydroxygenistein.aspergillus oryzae cytochrome p450 monooxygenase (cyp57b3) is capable of catalyzing hydroxylation of genistein to produce 3΄-hydroxygenistein. because hydroxylated derivatives of genistein, including 3΄-hydroxygenistein, exhibit various pharmacological activities, cyp57b3 would potentially be useful as a biocatalyst in the pharmaceutical field. we therefore performed random mutagenesis of cyp57b3 to improve its catalytic activities for genistein. random mutations were introduced by error-prone p ...201728520936
improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from aspergillus oryzae, aoxyn11a, by iterative mutagenesis based on in silico design.to improve the temperature characteristics and catalytic efficiency of a glycoside hydrolase family (ghf) 11 xylanase from aspergillus oryzae (aoxyn11a), its variants were predicted based on in silico design. firstly, gly(21) with the maximum b-factor value, which was confirmed by molecular dynamics (md) simulation on the three-dimensional structure of aoxyn11a, was subjected to site-saturation mutagenesis. thus, one variant with the highest thermostability, aoxyn11a(g21i), was selected from the ...201728508385
rewiring the reductive tricarboxylic acid pathway and l-malate transport pathway of aspergillus oryzae for overproduction of l-malate.aspergillus oryzae finds wide application in the food, feed, and wine industries, and is an excellent cell factory platform for production of organic acids. in this work, we achieved the overproduction of l-malate by rewiring the reductive tricarboxylic acid (rtca) pathway and l-malate transport pathway of a. oryzae nrrl 3488. first, overexpression of native pyruvate carboxylase and malate dehydrogenase in the rtca pathway improved the l-malate titer from 26.1gl(-1) to 42.3gl(-1) in shake flask ...201728506930
asp30 of aspergillus oryzae cutinase cutl1 is involved in the ionic interaction with fungal hydrophobin rola.aspergillus oryzae hydrophobin rola adheres to the biodegradable polyester polybutylene succinate-co-adipate (pbsa) and promotes pbsa degradation by interacting with a. oryzae polyesterase cutl1 and recruiting it to the pbsa surface. in our previous studies, we found that positively charged amino acid residues (h32, k34) of rola and negatively charged residues (e31, d142, d171) of cutl1 are important for the cooperative ionic interaction between rola and cutl1, but some other charged residues in ...201728475418
mitogenic lectins from cephalosporium curvulum (csl) and aspergillus oryzae (aol) mediate host-pathogen interactions leading to mycotic keratitis.a core-fucose-specific lectin, csl from cephalosporium curvulum, has been reported earlier. here we assign the role for csl and another lectin aol, from pathogenic fungus aspergillus oryzae, in causing mycotic keratitis. csl and aol show strong binding to immortalized and primary human corneal epithelial cells (hcecs) which are inhibited by asialofetuin, confirming their glycan-mediated binding. csl and aol showed increase in viability at lower concentrations (0.07 µg/ml) whereas at higher conce ...201728470344
comprehensive analysis of fungal diversity and enzyme activity in nuruk, a korean fermenting starter, for acquiring useful fungi.nuruk is a fermenting starter that is involved in the production of alcoholic beverages, and has been used in south korea for a very long time. to analyze the fungal diversity, we collected a total of 59 nuruk samples from several companies and persons in 2013 to 2014, and obtained 364 isolates. all of the single isolated fungi were identified, both morphologically and molecularly, based on the sequences of ribosomal rna gene [18s, its1-5.8s-its2, and 26s (d1/d2 region)]. in 46 nuruk samples out ...201728455587
engineered humicola insolens cutinase for efficient cellulose acetate deacetylation.cutinases comprise a family of esterases with broad hydrolytic activity for chain and pendant ester groups. this work aimed to identify and improve an efficient cutinase for cellulose acetate (ca) deacetylation. the development of a mild method for ca fiber surface deacetylation will result in improved surface hydrophilicity and reactivity while, when combined with cellulases, a route to the full recycling of ca to acetate and glucose. in this study, the comparative ca deacetylation activity of ...201728488758
highly efficient gene targeting in aspergillus oryzae industrial strains under ligd mutation introduced by genome editing: strain-specific differences in the effects of deleting ecdr, the negative regulator of sclerotia formation.numerous strains of aspergillus oryzae are industrially used for japanese traditional fermentation and for the production of enzymes and heterologous proteins. in a. oryzae, deletion of the ku70 or ligd genes involved in non-homologous end joining (nhej) has allowed high gene targeting efficiency. however, this strategy has been mainly applied under the genetic background of the a. oryzae wild strain rib40, and it would be laborious to delete the nhej genes in many a. oryzae industrial strains, ...201728484116
improved α-amylase production by dephosphorylation mutation of cred, an arrestin-like protein required for glucose-induced endocytosis of maltose permease and carbon catabolite derepression in aspergillus oryzae.aspergillusoryzae produces copious amount of amylolytic enzymes, and malp, a major maltose permease, is required for the expression of amylase-encoding genes. the expression of these genes is strongly repressed by carbon catabolite repression (ccr) in the presence of glucose. malp is transported from the plasma membrane to the vacuole by endocytosis, which requires the homolog of e6-ap carboxyl terminus ubiquitin ligase hula, an ortholog of yeast rsp5. in yeast, arrestin-like proteins mediate en ...201728455339
performance of an ultrafiltration membrane bioreactor (uf-mbr) as a processing strategy for the synthesis of galacto-oligosaccharides at high substrate concentrations.the performance of an ultrafiltration membrane bioreactor for galacto-oligosaccharides (gos) synthesis using high lactose concentrations (470 g/l) and β-galactosidase from aspergillus oryzae was assessed. tested processing variables were: transmembrane-pressure (pt), crossflow-velocity (cfv) and temperature. results showed that processing variables had significant effect on the yield, the enzyme productivity and the flux but did not on gos concentration and reaction conversion obtained. as expec ...201626924238
microbial bioconversion and processing methods enhance the phenolic acid and flavonoids and the radical scavenging capacity of smilax china l. leaf.it has been reported that smilax china l. leaf (scl) provided various biological functions owing to polyphenols. the objective of the current study was to assess the enhancing effect of processing methods and microbial conversions on phenolic acid and flavonoid content and radical scavenging capacity of scl for potential applications of diverse food products.201625754764
thermostabilization of aspergillus oryzae β-d-galactosidase.the thermal stability of the aspergillus oryzae β-d-galactosidase (β-gal) was evaluated at 60 °c. the stability of β-gal was dependent on the ph, buffer, and additives. the β-gal exhibited its highest thermal stability in a 0.02 m phosphate buffer ph 6. among all the tested additives, galactose, a competitive inhibitor of β-gal, was the upmost thermal stabilizer. a 0.15 m galactose solution caused β-gal to retain a whole of 98.65% of its initial activity after incubation at 60 °c for 2 h. the se ...201626011544
production of the natural iron chelator deferriferrichrysin from aspergillus oryzae and evaluation as a novel food-grade antioxidant.deferriferrichrysin (dfcy) is a siderophore found in foods fermented by aspergillus oryzae and is a promising candidate for an antioxidant food additive because of its high binding constant toward iron. however, the dfcy concentration is typically low in foods and cultures.201626399367
purification and enzymatic characterization of a novel β-1,6-glucosidase from aspergillus oryzae.in this study, among the 10 genes that encode putative β-glucosidases in the glycoside hydrolase family 3 (gh3) with a signal peptide in the aspergillus oryzae genome, we found a novel gene (ao090038000425) encoding β-1,6-glucosidase with a substrate specificity for gentiobiose. the transformant harboring ao090038000425, which we named bglh, was overexpressed under the control of the improved glaa gene promoter to form a small clear zone around the colony in a plate assay using 4-methylumbellife ...201626320404
heterologous expression and characterization of a thermostable exo-β-d-glucosaminidase from aspergillus oryzae.an exo-β-d-glucosaminidase (aorcsxa) from aspergillus oryzae fl402 was heterologously expressed and purified. the deduced amino acid sequence indicated that aorcsxa belonged to glycoside hydrolase family 2. aorcsxa digested colloid chitosan into glucosamine but not into chitosan oligosaccharides, demonstrating exo-β-d-glucosaminidase (csxa) activity. aorcsxa exhibited optimal activity at ph 5.5 and 50°c; however, the enzyme expressed in pichia pastoris (ppaorcsxa) showed much stronger thermostab ...201626597529
aorim15 is involved in conidial stress tolerance, conidiation and sclerotia formation in the filamentous fungus aspergillus oryzae.the serine-threonine kinase rim15p is a master regulator of stress signaling and is required for stress tolerance and sexual sporulation in the yeast saccharomyces cerevisiae. however, in filamentous fungi that reproduce asexually via conidiation, the physiological function of rim15p homologs has not been extensively analyzed. here, we functionally characterized the protein homolog of rim15p in the filamentous fungus aspergillus oryzae, by deleting and overexpressing the corresponding aorim15 ge ...201626467693
aspergillus oryzae fermented germinated soybean extract alleviates perimenopausal symptoms in ovariectomised rats.soybeans have been widely used to alleviate climacteric symptoms. in this study, we investigated the oestrogenic activities of isoflavones extracted from aspergillus oryzae-challenged germinated soybeans (ao-gs). eight-week-old virgin sprague-dawley female rats were ovariectomised (ovx). the rats were orally administered 0.1 mg kg(-1) 17α-ethinyl oestradiol or three different doses of ao-gs (0.5, 1.0 2.0 g kg(-1) day(-1)) in distilled water for 6 weeks, while control rats were administered vehic ...201625777819
toward rational thermostabilization of aspergillus oryzae cutinase: insights into catalytic and structural stability.cutinases are powerful hydrolases that can cleave ester bonds of polyesters such as poly(ethylene terephthalate) (pet), opening up new options for enzymatic routes for polymer recycling and surface modification reactions. cutinase from aspergillus oryzae (aoc) is promising owing to the presence of an extended groove near the catalytic triad which is important for the orientation of polymeric chains. however, the catalytic efficiency of aoc on rigid polymers like pet is limited by its low thermos ...201626522152
a long natural-antisense rna is accumulated in the conidia of aspergillus oryzae.analysis of expressed sequence tag libraries from various culture conditions revealed the existence of conidia-specific transcripts assembled to putative conidiation-specific reductase gene (csra) in aspergillus oryzae. however, the all transcripts were transcribed with opposite direction to the gene csra. the sequence analysis of the transcript revealed that the rna overlapped mrna of csra with 3'-end, and did not code protein longer than 60 amino acid residues. we designated the transcript con ...201626539735
evaluation of kojic acid production in a repeated-batch pcs biofilm reactor.in this study, kojic acid, a secondary metabolite as an industrially important compound, was produced by aspergillus oryzae (a. oryzae), which was immobilized in plastic composite support (pcs) bioreactor. nitrogen deficient medium was applied to increase the production of ka in pcs-immobilized bioreactor. the efficiency of immobilized culture for kojic acid (ka) production and the effect of morphology of a. oryzae on ka production were evaluated. after three cycles of cultivation, 83.47 g/l of ...201626657710
a biosensor based on gold nanoparticles stabilized in poly(allylamine hydrochloride) and decorated with laccase for determination of dopamine.gold nanoparticles stabilized in poly(allylamine hydrochloride) (aunp-pah) were used as a support for the immobilization of the enzyme laccase obtained from genetically-modified microorganisms (aspergillus oryzae) and successfully applied in the development of a new biosensor for the determination of dopamine by square-wave voltammetry. the electrochemical characterization of the biosensor was performed by cyclic voltammetry and electrochemical impedance spectroscopy and indicated that the nanom ...201626566574
occupational sensitization to lactase in the dietary supplement industry.aerogen lactase exposure carries a risk for the development of allergic asthma and rhinitis; only a few occupationally affected patients have been reported. the authors report the results of allergy testing with employees of a lactase tablets manufacturing plant. the survey involved 13 workers, including a questionnaire, spirometry, basophil activation test (bat), and skin prick tests (spts) with lactase and a panel of common aeroallergens. furthermore, lactase-specific immunoglobulin e (ige) an ...201626134755
stereoselective behavior of the chiral herbicides diclofop-methyl and diclofop during the soy sauce brewing process.chiral pesticides are now receiving more and more attention in the food-making process. this experiment studied the enantioselective behavior of diclofop-methyl (dm) and its main metabolite, diclofop (dc), during the soy sauce brewing process. two kinds of commonly used strains, aspergillus oryzae and saccharomyces rouxii, were investigated. however, they showed a different degradation ability to the enantiomers of dm and dc. it was observed that (-)-(s)-dm was degraded much faster than (+)-(r)- ...201626501920
multi-response optimisation of the extraction solvent system for phenolics and antioxidant activities from fermented soy flour using a simplex-centroid design.a simplex-centroid design comprising three solvents (water, ethanol and methanol) was used to optimise the extraction mixture for phenolics and antioxidant activities from defatted soy flour fermented with monascus purpureus or aspergillus oryzae. total phenolics were more efficiently extracted using only water for both samples. the highest antioxidant activities by the dpph and abts methods were obtained using extraction mixtures containing at least 75 wt% water. specific water:ethanol:methanol ...201626616938
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