Publications

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application of dyes extracted from alternanthera dentata leaves and musa acuminata bracts as natural sensitizers for dye-sensitized solar cells.the natural dyes anthocyanin and chlorophyll were extracted from musa acuminata bracts and alternanthera dentata leaves, respectively. the dyes were then applied as sensitizers in tio2-based dye-sensitized solar cells (dsscs). the ethanol extracts of the dyes had maximum absorbance. high dye yields were obtained under extraction temperatures of 70 to 80°c, and the optimal extraction temperature was approximately 80°c. moreover, dye concentration sharply decreased under extraction temperatures th ...201829133132
employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana.diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. the introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. banana fruit (musa acuminata) is a well-established allergen source which besides six characterized allergens, contains unidentified ige reactive proteins whose clinical relevance remains undefined. by employment of a combinatorial peptide li ...201829331514
further insights into the metal ion binding abilities and the metalation pathway of a plant metallothionein from musa acuminata.the superfamily of metallothioneins (mts) combines a diverse group of metalloproteins, sharing the characteristics of rather low molecular weight and high cysteine content. the latter provides mts with the capability to coordinate thiophilic metal ions, in particular those with a d 10 electron configuration. the sub-family of plant mt3 proteins is only poorly characterized and there is a complete lack of three-dimensional structure information. building upon our previous results on the musa acum ...201829218632
a high throughput molecular pipeline reveals the diversity in prevalence and abundance of pratylenchus and meloidogyne species in coffee plantations.coffee yields are adversely affected by plant-parasitic nematodes and the pathogens are largely under-reported because a simple and reliable identification method is not available. we describe a pcr-based approach to rapidly detect and quantify the major pratylenchus and meloidogyne nematode species that are capable of parasitising coffee. the procedure was applied to soil samples obtained from a number of coffee farms in brazil, vietnam and indonesia to assess the prevalence of these species as ...201829291356
characterization and amplification of gene-based simple sequence repeat (ssr) markers in date palm.the paucity of molecular markers limits the application of genetic and genomic research in date palm (phoenix dactylifera l.). availability of expressed sequence tag (est) sequences in date palm may provide a good resource for developing gene-based markers. this study characterizes a substantial fraction of transcriptome sequences containing simple sequence repeats (ssrs) from the est sequences in date palm. the est sequences studied are mainly homologous to those of elaeis guineensis and musa a ...201728755229
antioxidant effcacy of unripe banana (musa acuminata colla) peel extracts in sunflower oil during accelerated storage.sunflower oil is prone to oxidation during storage time, leading to production of toxic compounds that might affect human health. synthetic antioxidants are used to prevent lipid oxidation. spreading interest in the replacement of synthetic food antioxidants by natural ones has fostered research on fruit and vegetables for new antioxidants.201728068040
a tree of life based on ninety-eight expressed genes conserved across diverse eukaryotic species.rapid advances in dna sequencing technologies have resulted in the accumulation of large data sets in the public domain, facilitating comparative studies to provide novel insights into the evolution of life. phylogenetic studies across the eukaryotic taxa have been reported but on the basis of a limited number of genes. here we present a genome-wide analysis across different plant, fungal, protist, and animal species, with reference to the 36,002 expressed genes of the rice genome. our analysis ...201728922368
carbon-doped mesoporous anatase tio2 multi-tubes nanostructures for highly improved visible light photocatalytic activity.development of a high surface area and efficient visible light induced photocatalyst on a large scale is a promising task from the practical perspective. in this study, visible light active c-doped anatase tio2 multi-tubes were synthesized using banana (musa acuminata) stem fiber as a sacrificial template, removed by calcination at 450 °c. during the calcination process, the lattice of anatase tio2 phase was doped with c, and obtained multi-tubes showed high surface area (∼99 m2/g) with a mesopo ...201728783348
the agpase family proteins in banana: genome-wide identification, phylogeny, and expression analyses reveal their involvement in the development, ripening, and abiotic/biotic stress responses.adp-glucose pyrophosphorylase (agpase) is the first rate-limiting enzyme in starch biosynthesis and plays crucial roles in multiple biological processes. despite its importance, agpase is poorly studied in starchy fruit crop banana (musa acuminata l.). in this study, eight maagpase genes have been identified genome-wide in m. acuminata, which could be clustered into the large (apl) and small (aps) subunits. comprehensive transcriptomic analysis revealed temporal and spatial expression variations ...201728757545
genome-wide analysis of banana mads-box family closely related to fruit development and ripening.proteins encoded by mads-box genes are important transcription factors involved in the regulation of flowering plant growth and development. currently, no systematic information exists regarding the mads-box family in the important tropical fruit banana. ninety-six mads-box genes were identified from the banana (pahang) a genome. phylogenetic analysis indicated that musa acuminata mcm1-agamous- deficiens-srf (mamads) could be divided into mikcc, mikc*, mα/β and mγ groups. mikcc could be further ...201728615681
evolution of the banana genome (musa acuminata) is impacted by large chromosomal translocations.most banana cultivars are triploid seedless parthenocarpic clones derived from hybridization between musa acuminata subspecies and sometimes m. balbisiana. m. acuminata subspecies were suggested to differ by a few large chromosomal rearrangements based on chromosome pairing configurations in intersubspecies hybrids. we searched for large chromosomal rearrangements in a seedy m. acuminata ssp. malaccensis banana accession through mate-pair sequencing, bac-fish, targeted pcr and marker (dartseq) s ...201728575404
soluble starch synthase iii-1 in amylopectin metabolism of banana fruit: characterization, expression, enzyme activity, and functional analyses.soluble starch synthase (ss) is one of the key enzymes involved in amylopectin biosynthesis in plants. however, no information is currently available about this gene family in the important fruit crop banana. herein, we characterized the function of massiii-1 in amylopectin metabolism of banana fruit and described the putative role of the other mass family members. firstly, starch granules, starch and amylopectin content were found to increase during banana fruit development, but decline during ...201728424724
effect of thai banana (musa aa group) in reducing accumulation of oxidation end products in uvb-irradiated mouse skin.chronic uvb exposure causes skin disorders and cancer through dna strand breaks and oxidation of numerous functional groups of proteins and lipids in the skin. in this study, we investigated the effects of thai banana (musa aa group, "khai," and musa abb group, "namwa") on the prevention of uvb-induced skin damage when fed to male icr mice.201728187402
phlebotomine sandfly ecology on the indian subcontinent: does village vegetation play a role in sandfly distribution in bihar, india?visceral leishmaniasis (vl) is a disease that results in approximately 50 000 human deaths annually. it is transmitted through the bites of phlebotomine sandflies and around two-thirds of cases occur on the indian subcontinent. indoor residual spraying (irs), the efficacy of which depends upon sandfly adults resting indoors, is the only sandfly control method used in india. recently, in bihar, india, considerable sandfly numbers have been recorded outdoors in village vegetation, which suggests t ...201728106262
the effect of blue light on stomatal oscillations and leaf turgor pressure in banana leaves.stomatal oscillations are cyclic opening and closing of stomata, presumed to initiate from hydraulic mismatch between leaf water supply and transpiration rate. to test this assumption, mismatches between water supply and transpiration were induced using manipulations of vapour pressure deficit (vpd) and light spectrum in banana (musa acuminata). simultaneous measurements of gas exchange with changes in leaf turgor pressure were used to describe the hydraulic mismatches. an increase of vpd above ...201728098339
the transcriptional regulatory network mediated by banana (musa acuminata) dehydration-responsive element binding (madreb) transcription factors in fruit ripening.fruit ripening is a complex, genetically programmed process involving the action of critical transcription factors (tfs). despite the established significance of dehydration-responsive element binding (dreb) tfs in plant abiotic stress responses, the involvement of drebs in fruit ripening is yet to be determined. here, we identified four genes encoding ripening-regulated dreb tfs in banana (musa acuminata), madreb1, madreb2, madreb3, and madreb4, and demonstrated that they play regulatory roles ...201728044313
use of banana (musa acuminata colla aaa) peel extract as an antioxidant source in orange juices.using banana peel extract as an antioxidant in freshly squeezed orange juices and juices from concentrate was evaluated. free radical scavenging capacity increased by adding banana peel extracts to both types of orange juice. in addition, remarkable increases in antioxidant capacity using 2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (abts) radical were observed when equal or greater than 5 mg of banana peel extract per ml of freshly squeezed juice was added. no clear effects were obse ...201727987069
long noncoding rnas that respond to fusarium oxysporum infection in 'cavendish' banana (musa acuminata).long noncoding rnas (lncrnas) are a class of genes that influence a variety of biological functions through acting as signal, decoy, guide, and scaffold molecules. in banana (musa spp.), an important economic fruit crop, particularly in southeast asia, the wilt disease caused by fusarium oxysporum f. sp. cubense (foc), especially strain foc tr4, is disastrous. in banana, how the biogenesis of these lncrnas is regulated in response to pathogen infection is still largely unknown. in this study, st ...201729209086
codon usage and codon pair patterns in non-grass monocot genomes.studies on codon usage in monocots have focused on grasses, and observed patterns of this taxon were generalized to all monocot species. here, non-grass monocot species were analysed to investigate the differences between grass and non-grass monocots.201729155926
effects of aqueous extract of three cultivars of banana (musa acuminata) fruit peel on kidney and liver function indices in wistar rats.background:musa acuminata fruit peels are used in the northern part of nigeria for the treatment of hypertension and other cardiovascular related diseases. the effects of aqueous extracts of ripped fruit peel of three cultivars of musa acuminata (saro, ominni and oranta) on the hepatic and renal parameters of normal rats were examined. methods: fruit peel aqueous extracts (fpae) of the 3 cultivars of bananas (100 mg/kg b.w.) were administered by oral intubation (that is through esophageal cannul ...201729065553
leaves from banana (musa nana) and maize (zea mays) have no phyto-prophylactic effects on the susceptibility of grass carp (ctenopharyngodon idella) to aeromonas hydrophila infection.the ubiquitous and opportunistic bacterial pathogen aeromonas hydrophila has been associated with ulcerative dermatitis in fish, especially under stressful conditions. it can cause severe losses in fresh water aquaculture and is particularly prevalent in tropical and subtropical regions. fresh leaves from maize and bananas have been used as feed supplement by fish farmers in vietnam and it has been reported that they may have phyto-prophylactic benefits. in the present study, a feeding trial was ...201729126413
genome-wide analysis of autophagy-related genes in banana highlights maatg8s in cell death and autophagy in immune response to fusarium wilt.maatg8s play important roles in hypersensitive-like cell death and immune response, and autophagy is essential for disease resistance against foc in banana. autophagy is responsible for the degradation of damaged cytoplasmic constituents in the lysosomes or vacuoles. although the effects of autophagy have been extensively revealed in model plants, the possible roles of autophagy-related gene in banana remain unknown. in this study, 32 maatgs were identified in the draft genome, and the profiles ...201728451821
local phytochemical response of musa acuminata × balbisiana colla cv. 'bluggoe' (abb) to colonization by sternorrhyncha.the interaction of two sternorrhyncha species, the banana aphid (pentalonia nigronervosa coquerel (hemiptera: aphididae, aphidinae)), vector of the banana bunchy top virus (bbtv), and the latania scale (hemiberlesia lataniae signoret (hemiptera: diaspididae, diaspidinae)) with musa acuminata × balbisiana colla (abb group) 'bluggoe' (musaceae) was investigated by a combination of conventional and spatially resolved analytical techniques, (1)h nmr, uhplc-ms, and matrix-free uv-laser desorption/ion ...201727839785
identification, transcriptional and functional analysis of heat-shock protein 90s in banana (musa acuminata l.) highlight their novel role in melatonin-mediated plant response to fusarium wilt.as one popular fresh fruit, banana (musa acuminata) is cultivated in the world's subtropical and tropical areas. in recent years, pathogen fusarium oxysporum f. sp. cubense (foc) has been widely and rapidly spread to banana cultivated areas, causing substantial yield loss. however, the molecular mechanism of banana response to foc remains unclear, and functional identification of disease-related genes is also very limited. in this study, nine 90 kda heat-shock proteins (hsp90s) were genomewide i ...201727627033
gene expression analysis in musa acuminata during compatible interactions with meloidogyne incognita.endoparasitic root-knot nematodes (rkns) (meloidogyne spp.) cause considerable losses in banana (musa spp.), with meloidogyne incognita a predominant species in cavendish sub-group bananas. this study investigates the root transcriptome in musa acuminata genotypes 4297-06 (aa) and cavendish grande naine (cav; aaa) during early compatible interactions with m. incognita methods: roots were analysed by brightfield light microscopy over a 35 d period to examine nematode penetration and morphological ...201728130221
evolution and structural diversification of nictaba-like lectin genes in food crops with a focus on soybean (glycine max).the nictaba family groups all proteins that show homology to nictaba, the tobacco lectin. so far, nictaba and an arabidopsis thaliana homologue have been shown to be implicated in the plant stress response. the availability of more than 50 sequenced plant genomes provided the opportunity for a genome-wide identification of nictaba-like genes in 15 species, representing members of the fabaceae, poaceae, solanaceae, musaceae, arecaceae, malvaceae and rubiaceae. additionally, phylogenetic relations ...201728087663
label-free potentiometric biosensor based on solid-contact for determination of total phenols in honey and propolis.we developed a label-free potentiometric biosensor using tyrosinase extracted from musa acuminata and immobilized by covalent bond on a surface of a solid-contact transducer. the transducer was manufactured containing two layers. the first layer contained a blend of poly(vinyl) chloride carboxylated (pvc-cooh), graphite and potassium permanganate. on this layer, we deposited a second layer containing just a mixture of poly(vinyl chloride) carboxylated and graphite. on the last layer of the trans ...201728107950
two banana cultivars differ in composition of potentially immunomodulatory mannan and arabinogalactan.banana (musa acuminata and m. acuminata x m. balbisiana) fruit cell walls are rich in mannans, homogalacturonans and xylogalacturonan, rhamnogalacturonan-i, and arabinogalactans, certain forms of which is considered to have immunomodulatory activity. the cultivars nanicão and thap maeo represent two widely variants with respect to compositional differences in the forms of these polysaccharides. nanicão has low amounts of mannan in the water-insoluble and water-soluble fraction. both cultivars ha ...201728325331
a combined spectroscopic and tddft study of natural dyes extracted from fruit peels of citrus reticulata and musa acuminata for dye-sensitized solar cells.this study reports the novel spectroscopic investigations and enhanced the electron transfers of citrus reticulata and musa acuminata fruit peels as the photosensitizers for the dye-sensitized solar cells. the calculated td-dft-ub3lyp/6-31+g(d,p)-iefpcm(uaks), experiment spectra of ultra-violet-visible spectroscopy, and fourier transform infrared spectroscopy studies indicate the main flavonoid (hesperidin and gallocatechin) structures of the dye extracts. the optimized flavonoid structures are ...201727497289
field evidence of strong differential pollen placement by old world bat-pollinated plants.sympatric plant species that share pollinators potentially compete for pollination and risk interspecific pollen transfer, but this competition can be minimized when plant species place pollen on different areas of the pollinator's body. multiple studies have demonstrated strong differential pollen placement by sympatric plant species under laboratory conditions; however, field evidence collected in natural settings is less common. furthermore, it is unknown whether precise pollen placement on t ...201727864223
xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east africa over the past decade.taxonomy: bacteria; phylum proteobacteria; class gammaproteobacteria; order xanthomonadales; family xanthomonadaceae; genus xanthomonas; currently classified as x. campestris pv. musacearum (xcm). however, fatty acid methyl esters analysis, genetic and genomic evidence suggest that this pathogen is x. vasicola and resides in a separate pathovar. isolation and detection: xcm can be isolated on yeast peptone glucose agar (ypga), cellobiose cephalexin agar and yeast tryptone sucrose agar (ytsa) com ...201728677256
infestation of raoiella indica hirst (trombidiformes: tenuipalpidae) on host plants of high socio-economic importance for tropical america.the mite raoiella indica hirst was recently introduced into america, where it has shown amazing ability to disseminate and broaden its range of hosts. an experiment was conducted in cancún, mexico, to determine infestation levels of this mite on plants recorded as hosts: coconut palm (cocos nucifera) of cultivars pacific tall and malayan dwarf, oil palm (elaeis guineensis) hybrids deli x ghana and deli x nigeria, dwarf giant banana (musa acuminata, aaa subgroup cavendish), horn plantain (m. acum ...201626874954
computational identification and comparative analysis of mirna precursors in three palm species.in the present study, mirna precursors in the genomes of three palm species were identified. analyzes of sequence conservation and biological function of their putative targets contribute to understand the roles of mirna in palm biology. micrornas are small rnas of 20-25 nucleotides in length, with important functions in the regulation of gene expression. recent genome sequencing of the palm species elaeis guineensis, elaeis oleifera and phoenix dactylifera have enabled the discovery of mirna ge ...201626919984
evaluation of terrestrial plants extracts for uranium sorption and characterization of potent phytoconstituents.sorption capacity of four plants (funaria hygrometrica, musa acuminata, brassica juncea and helianthus annuus) extracts/fractions for uranium, a radionuclide was investigated by edxrf and tracer studies. the maximum sorption capacity, i.e., 100% (complete sorption) was observed in case of musa acuminata extract and fractions. carbohydrate, proteins, phenolics and flavonoids contents in the active fraction (having maximum sorption capacity) were also determined. further purification of the most a ...201625946322
differential pollen placement on an old world nectar bat increases pollination efficiency.plant species that share pollinators are potentially subject to non-adaptive interspecific pollen transfer, resulting in reduced reproductive success. mechanisms that increase pollination efficiency between conspecific individuals are therefore highly beneficial. many nocturnally flowering plant species in thailand are pollinated by the nectar bat eonycteris spelaea (pteropodidae). this study tested the hypothesis that plant species within a community reduce interspecific pollen movement by plac ...201626482654
heat shock transcription factors in banana: genome-wide characterization and expression profile analysis during development and stress response.banana (musa acuminata) is one of the most popular fresh fruits. however, the rapid spread of fungal pathogen fusarium oxysporum f. sp. cubense (foc) in tropical areas severely affected banana growth and production. thus, it is very important to identify candidate genes involved in banana response to abiotic stress and pathogen infection, as well as the molecular mechanism and possible utilization for genetic breeding. heat stress transcription factors (hsfs) are widely known for their common in ...201627857174
wild banana seed phytobezoar rectal impaction causing intestinal obstruction.wild banana (musa acuminata subsp. microcarpa) seed phytobezoar rectal impaction in adult is a rare entity. here, we report a 75-year-old male with dementia who presented with lower abdominal pain, per-rectal bleeding and overflow faecal incontinence. our investigation discovered a large wild banana seed phytobezoar impacted in the rectum causing intestinal obstruction, stercoral ulcer and faecal overflow incontinence. in this article, we discuss the patient's clinical findings, imaging and mana ...201627574355
comparative study of the physicochemical, compositional and functional properties of eight flours obtained from different plant materials found in colombia.the physicochemical, compositional and functional properties of flour from green plantains (musa acuminata) of the large green plantain variety, oyster mushrooms (pleorotus ostreatus), pineapple peel (ananas comosus) of the 'apple pineapple' variety, yellow peas (pisum sativum), chickpeas (cicer arietinum), whole grain rice (oryza sativa), whole grain corn (zea mays) and whole grain white quinoa (chenopodium quinoa) were evaluated by using one-way analysis of variance, pearson correlations and p ...201627091150
molecular characterisation of endophytic fungi from roots of wild banana (musa acuminata).endophytic fungi inhabit apparently healthy plant tissues and are prevalent in terrestrial plants, especially root tissues, which harbour a wide assemblage of fungal endophytes. therefore, this study focused on the isolation and characterisation of endophytic fungi from the roots of wild banana (musa acuminata). a total of 31 isolates of endophytic fungi were isolated from 80 root fragments. the endophytic fungi were initially sorted according to morphological characteristics and identified usin ...201627019688
dietary administration of banana (musa acuminata) peel flour affects the growth, antioxidant status, cytokine responses, and disease susceptibility of rohu, labeo rohita.to explore the feasibility of musa acuminata (banana) peels as a feed additive, effects of banana peel flour (bpf) on the growth and immune functions of labeo rohita were evaluated. diets containing five different concentrations of bpf (0% [basal diet], 1% [b1], 3% [b3], 5% [b5], and 7% [b7]) were fed to the fish (average weight: 15.3 g) for 60 days. the final weight gain and specific growth rate were higher (p < 0.05) in the b5 group. the most significant improvements in immune parameters such ...201627294156
extraction and partial characterization of polyphenol oxidase from banana (musa acuminata grande naine) roots.polyphenol oxidase activity (ppo, ec 1.14.18.1, monophenol monooxygenase, and ec 1.10.3.2, o-diphenoloxidase) has been extensively studied in banana fruit for its role in enzymatic browning. rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts indicate that ppo and phenolic compounds are ubiquitous in vegetative tissue of banana as well. they hamper biochemical and molecular studies in banana, as cumbersome adaptations of extraction protocols ...201616814556
transcriptome sequencing of mycosphaerella fijiensis during association with musa acuminata reveals candidate pathogenicity genes.mycosphaerella fijiensis, causative agent of the black sigatoka disease of banana, is considered the most economically damaging banana disease. despite its importance, the genetics of pathogenicity are poorly understood. previous studies have characterized polyketide pathways with possible roles in pathogenicity. to identify additional candidate pathogenicity genes, we compared the transcriptome of this fungus during the necrotrophic phase of infection with that during saprophytic growth in medi ...201627576702
improvement of the banana "musa acuminata" reference sequence using ngs data and semi-automated bioinformatics methods.recent advances in genomics indicate functional significance of a majority of genome sequences and their long range interactions. as a detailed examination of genome organization and function requires very high quality genome sequence, the objective of this study was to improve reference genome assembly of banana (musa acuminata).201626984673
traditional banana diversity in oceania: an endangered heritage.this study aims to understand the genetic diversity of traditional oceanian starchy bananas in order to propose an efficient conservation strategy for these endangered varieties. ssr and dart molecular markers are used to characterize a large sample of pacific accessions, from new guinea to tahiti and hawaii. all pacific starchy bananas are shown of new guinea origin, by interspecific hybridization between musa acuminata (aa genome), more precisely its local subspecies m. acuminata ssp. banksii, ...201626982801
the banana fruit dof transcription factor madof23 acts as a repressor and interacts with maerf9 in regulating ripening-related genes.the dna binding with one finger (dof) proteins, a family of plant-specific transcription factors, are involved in a variety of plant biological processes. however, little information is available on their involvement in fruit ripening. we have characterized 25 madof genes from banana fruit (musa acuminata), designated as madof1-madof25 gene expression analysis in fruit subjected to different ripening conditions revealed that madofs were differentially expressed during different stages of ripenin ...201626889012
characteristics of three thioredoxin genes and their role in chilling tolerance of harvested banana fruit.thioredoxins (trxs) are small proteins with a conserved redox active site wcgpc and are involved in a wide range of cellular redox processes. however, little information on the role of trx in regulating low-temperature stress of harvested fruit is available. in this study, three full-length trx cdnas, designated matrx6, matrx9 and matrx12, were cloned from banana (musa acuminata) fruit. phylogenetic analysis and protein sequence alignments showed that matrx6 was grouped to h2 type with a typical ...201627618038
antioxidant and antihyperglycemic properties of three banana cultivars (musa spp.).background. this study sought to investigate the antioxidant and antihyperglycemic properties of musa sapientum (latundan banana) (msl), musa acuminata (cavendish banana) (mac), and musa acuminate (red dacca) (mar). materials and methods. the sugar, starch, amylose, and amylopectin contents and glycemic index (gi) of the three banana cultivars were determined. furthermore, total phenol and vitamin c contents and α-amylase and α-glucosidase inhibitory effects of banana samples were also determine ...201627872791
transcriptomic changes of drought-tolerant and sensitive banana cultivars exposed to drought stress.in banana, drought responsive gene expression profiles of drought-tolerant and sensitive genotypes remain largely unexplored. in this research, the transcriptome of drought-tolerant banana cultivar (saba, abb genome) and sensitive cultivar (grand naine, aaa genome) was monitored using mrna-seq under control and drought stress condition. a total of 162.36 million reads from tolerant and 126.58 million reads from sensitive libraries were produced and mapped onto the musa acuminata genome sequence ...201627867388
translating the "banana genome" to delineate stress resistance, dwarfing, parthenocarpy and mechanisms of fruit ripening.evolutionary frozen, genetically sterile and globally iconic fruit "banana" remained untouched by the green revolution and, as of today, researchers face intrinsic impediments for its varietal improvement. recently, this wonder crop entered the genomics era with decoding of structural genome of double haploid pahang (aa genome constitution) genotype of musa acuminata. its complex genome decoded by hybrid sequencing strategies revealed panoply of genes and transcription factors involved in the pr ...201627833619
revisiting the zingiberales: using multiplexed exon capture to resolve ancient and recent phylogenetic splits in a charismatic plant lineage.the zingiberales are an iconic order of monocotyledonous plants comprising eight families with distinctive and diverse floral morphologies and representing an important ecological element of tropical and subtropical forests. while the eight families are demonstrated to be monophyletic, phylogenetic relationships among these families remain unresolved. neither combined morphological and molecular studies nor recent attempts to resolve family relationships using sequence data from whole plastomes ...201626819846
genome-wide analysis of the ap2/erf family in musa species reveals divergence and neofunctionalisation during evolution.ap2/erf domain containing transcription factor super family is one of the important regulators in the plant kingdom. the involvement of ap2/erf family members has been elucidated in various processes associated with plant growth, development as well as in response to hormones, biotic and abiotic stresses. in this study, we carried out genome-wide analysis to identify members of ap2/erf family in musa acuminata (a genome) and musa balbisiana (b genome) and changes leading to neofunctionalisation ...201626733055
genome-wide analysis of the dna-binding with one zinc finger (dof) transcription factor family in bananas.dna-binding with one finger (dof) domain proteins are a multigene family of plant-specific transcription factors involved in numerous aspects of plant growth and development. in this study, we report a genome-wide search for musa acuminata dof (madof) genes and their expression profiles at different developmental stages and in response to various abiotic stresses. in addition, a complete overview of the dof gene family in bananas is presented, including the gene structures, chromosomal locations ...201627831816
dart whole genome profiling provides insights on the evolution and taxonomy of edible banana (musa spp.).dessert and cooking bananas are vegetatively propagated crops of great importance for both the subsistence and the livelihood of people in developing countries. a wide diversity of diploid and triploid cultivars including aa, ab, as, at, aaa, aab, abb, aas and aat genomic constitutions exists. within each of this genome groups, cultivars are classified into subgroups that are reported to correspond to varieties clonally derived from each other after a single sexual event. the number of those fou ...201627590334
phenylphenalenones accumulate in plant tissues of two banana cultivars in response to herbivory by the banana weevil and banana stem weevil.phenylphenalenone-type compounds accumulated in the tissues of two banana cultivars-musa acuminata cv. "grande naine" (aaa) and musa acuminata × balbisiana colla cv. "bluggoe" (abb)-when these were fed on by the banana weevil (cosmopolites sordidus (germ.) (coleoptera: curculionidae)) and the banana stem weevil (odoiporus longicollis (oliver) (coleoptera: curculionidae)). the chemical constituents of the banana material were separated by means of chromatographic techniques and identified by nmr ...201627571112
genome-wide expression analysis and metabolite profiling elucidate transcriptional regulation of flavonoid biosynthesis and modulation under abiotic stresses in banana.flavonoid biosynthesis is largely regulated at the transcriptional level due to the modulated expression of genes related to the phenylpropanoid pathway in plants. although accumulation of different flavonoids has been reported in banana, a staple fruit crop, no detailed information is available on regulation of the biosynthesis in this important plant. we carried out genome-wide analysis of banana (musa acuminata, aaa genome) and identified 28 genes belonging to 9 gene families associated with ...201627539368
whole genome sequencing of a banana wild relative musa itinerans provides insights into lineage-specific diversification of the musa genus.crop wild relatives are valuable resources for future genetic improvement. here, we report the de novo genome assembly of musa itinerans, a disease-resistant wild banana relative in subtropical china. the assembled genome size was 462.1 mb, covering 75.2% of the genome (615.2mb) and containing 32, 456 predicted protein-coding genes. since the approximate divergence around 5.8 million years ago, the genomes of musa itinerans and musa acuminata have shown conserved collinearity. gene family expans ...201627531320
the banana transcriptional repressor madear1 negatively regulates cell wall-modifying genes involved in fruit ripening.ethylene plays an essential role in many biological processes including fruit ripening via modulation of ethylene signaling pathway. ethylene response factors (erfs) are key transcription factors (tfs) involved in ethylene perception and are divided into ap2, rav, erf, and dreb sub-families. although a number of studies have implicated the involvement of dreb sub-family genes in stress responses, little is known about their roles in fruit ripening. in this study, we identified a dreb tf with a e ...201627462342
effects of particle size distribution on some physical, chemical and functional properties of unripe banana flour.the objective of this study was to examine the effect of particle size distribution on physical, chemical and functional properties of unripe banana flour for the first time. a pure triploid (aaa group) of musa acuminata subgroup cavendish (°brix;0.2, ph;4.73, titratable acidity; 0.56g/100g malic acid, total solids; 27.42%) which was supplied from gazipaşa, antalya, turkey from october 2014 to october 2015 was used. size fractions of <212, 212-315, 316-500 and 501-700μm were characterized for th ...201627451170
molecular characterization of constans-like (col) genes in banana (musa acuminata l. aaa group, cv. grand nain).the constans (co) family is an important regulator of flowering in photoperiod sensitive plants. but information regarding their role in day neutral plants is limited. we report identification of nine group i type constans-like (col) genes of banana and their characterization for their age dependent, diurnal and tissue-specific expression. our studies show that the group i genes are conserved in structure to members in other plants. expression of these genes shows a distinct circadian regulation ...201627186015
molecular characterization of maccs, a novel copper chaperone gene involved in abiotic and hormonal stress responses in musa acuminata cv. tianbaojiao.copper/zinc superoxide dismutases (cu/znsods) play important roles in improving banana resistance to adverse conditions, but their activities depend on the copper chaperone for superoxide dismutase (ccs) delivering copper to them. however, little is known about ccs in monocots and under stress conditions. here, a novel ccs gene (maccs) was obtained from a banana using reverse transcription pcr and rapid-amplification of cdna ends (race) pcr. sequence analyses showed that maccs has typical ccs do ...201627023517
genome-wide analysis of the musa wrky gene family: evolution and differential expression during development and stress.the wrky gene family plays an important role in the development and stress responses in plants. as information is not available on the wrky gene family in musa species, genome-wide analysis has been carried out in this study using available genomic information from two species, musa acuminata and musa balbisiana. analysis identified 147 and 132 members of the wrky gene family in m. acuminata and m. balbisiana, respectively. evolutionary analysis suggests that the wrky gene family expanded much b ...201627014321
banana mamads transcription factors are necessary for fruit ripening and molecular tools to promote shelf-life and food security.genetic solutions to postharvest crop loss can reduce cost and energy inputs while increasing food security, especially for banana (musa acuminata), which is a significant component of worldwide food commerce. we have functionally characterized two banana e class (sepallata3 [sep3]) mads box genes, mamads1 and mamads2, homologous to the tomato (solanum lycopersicum) rin-mads ripening gene. transgenic banana plants repressing either gene (via antisense or rna interference [rnai]) were created and ...201626956665
oral administration of banana lectin modulates cytokine profile and abundance of t-cell populations in mice.banana lectin (banlec) is a dimeric protein occurring in fruit pulp that modulates immune cell functioning in vitro. in order to assess the immune response in vivo, banlec from ripe banana (musa acuminata) fruit was purified and orally given to mice for seven days. the analysis of cytokines in the mice peripheral blood revealed increased il-10, il-17 and tnfα, and a reduction of ifnγ and il-6. in the thymus, an increase of cd4+ and a decrease of cd8+ t-cells were observed after oral administrati ...201627106589
artificial neural network modelling of the antioxidant activity and phenolic compounds of bananas submitted to different drying treatments.bananas (cv. musa nana and musa cavendishii) fresh and dried by hot air at 50 and 70°c and lyophilisation were analysed for phenolic contents and antioxidant activity. all samples were subject to six extractions (three with methanol followed by three with acetone/water solution). the experimental data served to train a neural network adequate to describe the experimental observations for both output variables studied: total phenols and antioxidant activity. the results show that both bananas are ...201525172734
genome-wide identification and characterization of the superoxide dismutase gene family in musa acuminata cv. tianbaojiao (aaa group).superoxide dismutase (sod) is an essential enzyme of the plant antioxidant system that responds to oxidative stresses caused by adverse conditions. banana is an important staple and economic crop in tropical and subtropical regions. however, its growth and yield are constantly affected by various abiotic stresses. to analyze the roles of distinct sod genes under various stresses, a detailed characterization and analysis of the sod gene family in cavendish banana is indispensable.201526486759
efficacy of rapd, issr and damd markers in assessment of genetic variability and population structure of wild musa acuminata colla.north east india is considered as one of the major biodiversity hotspots worldwide and centre of origin of several plant species including musa. musa acuminata colla is known to be one of the wild progenitors of cultivated bananas and plantains. three single primer based dna marker techniques viz., random amplified polymorphic dna (rapd), inter simple sequence repeat (issr) and directed amplification of minisatellites dna (damd) were used for diversity diagnostics among 25 genotypes of wild m. a ...201526261399
high resolution mass spectrometry imaging reveals the occurrence of phenylphenalenone-type compounds in red paracytic stomata and red epidermis tissue of musa acuminata ssp. zebrina cv. 'rowe red'.the banana epidermis and in particular their stomata are conducive sites for the penetration of pathogenic fungi which can severely limit global banana production. the red pseudostem of the ornamental banana musa acuminata ssp. zebrina cv. 'rowe red' was used to study the chemical constituents of the epidermal cell layer using matrix-free laser desorption/ionization fourier transform ion cyclotron resonance mass spectrometric imaging (ldi-ft-icr-msi). the high resolution of this technique allowe ...201526004822
transcripts and micrornas responding to salt stress in musa acuminata colla (aaa group) cv. berangan roots.physiological responses to stress are controlled by expression of a large number of genes, many of which are regulated by micrornas. since most banana cultivars are salt-sensitive, improved understanding of genetic regulation of salt induced stress responses in banana can support future crop management and improvement in the face of increasing soil salinity related to irrigation and climate change. in this study we focused on determining mirna and their targets that respond to nacl exposure and ...201525993649
biochemical and in-silico studies on pectin methylesterase from g9 variety of musa acuminata for delayed ripening.ripening of fruit is a very important process but in some fruits early ripening leads to a great damage during long distance transportation. there are various biochemical changes taking place during the phase of ripening of fruit such as changes in respiration, aroma, flavor, ethylene production and activity of cell wall degrading enzymes. some important cell wall degrading enzymes are polygalacturonase (pg), pectin methylesterase (pme), pectin lyase, rgase. pme is known to act as a cell wall hy ...201525926894
efficient transformation of mycosphaerella fijiensis by underwater shock waves.black leaf streak disease, also known as black sigatoka, causes dramatic losses in production of banana and plantains fruits. the disease is caused by the pathogenic fungus mycosphaerella fijiensis (anamorph pseudocercospora fijiensis; mycosphaerellaceae). genetic transformation of m. fijiensis would allow a better understanding of molecular basis of pathogenicity and design novel approaches to control the infection caused by this pathogen. however, transformation of this fungus has not been eas ...201526456044
telomere-centric genome repatterning determines recurring chromosome number reductions during the evolution of eukaryotes.whole-genome duplication (wgd) is central to the evolution of many eukaryotic genomes, in particular rendering angiosperm (flowering plant) genomes much less stable than those of animals. following repeated duplication/triplication(s), angiosperm chromosome numbers have usually been restored to a narrow range, as one element in a 'diploidization' process that re-establishes diploid heredity. in several angiosperms affected by wgd, we show that chromosome number reduction (cnr) is best explained ...201525138576
banana ovate family protein maofp1 and mads-box protein mumads1 antagonistically regulated banana fruit ripening.the ovate family protein named maofp1 was identified in banana (musa acuminata l.aaa) fruit by a yeast two-hybrid (y2h) method using the banana mads-box gene mumads1 as bait and a 2 day postharvest (dph) banana fruit cdna library as prey. the interaction between mumads1 and maofp1 was further confirmed by y2h and bimolecular fluorescence complementation (bifc) methods, which showed that the mumads1 k domain interacted with maofp1. real-time quantitative pcr evaluation of mumads1 and maofp1 expre ...201525886169
a 3-vinyl cephem derivative, a useful intermediate in the synthesis of cepham antibiotics, from aspergillus awamori associated with banana fruit.aspergillus awamori was isolated from a diseased banana fruit, musa acuminata cv. ambul. the fungus was fermented in potato dextrose broth and on potato dextrose agar media and the fungal media were extracted with etoac. chromatographic separation of the etoac extracts furnished 4-methoxybenzyl 7-phenylacetamido-3-vinyl-3-cephem-4-carboxylate (1), along with three naphtho-γ-pyrones, flavasperone (2), foncesinone a (3) and aurasperone a (4), and three alkaloids, aspernigrin a (5), pestalamide c ( ...201526669099
activation of salicylic acid metabolism and signal transduction can enhance resistance to fusarium wilt in banana (musa acuminata l. aaa group, cv. cavendish).fusarium wilt caused by the fungus fusarium oxysporum f. sp. cubens (foc) is the most serious disease that attacks banana plants. salicylic acid (sa) can play a key role in plant-microbe interactions. our study is the first to examine the role of sa in conferring resistance to foc tr4 in banana (musa acuminata l. aaa group, cv. cavendish), which is the greatest commercial importance cultivar in musa. we used quantitative real-time reverse polymerase chain reaction (qrt-pcr) to analyze the expres ...201525277445
the effect of banana (musa acuminata) peels hot-water extract on the immunity and resistance of giant freshwater prawn, macrobrachium rosenbergii via dietary administration for a long term: activity and gene transcription.the non-specific immune parameters, disease resistance and immune genes expressions in macrobrachium rosenbergii were evaluated at 120 days of post feeding the diets containing the extracts of banana, musa acuminate, fruit's peel (banana peels extract, bpe) at 0, 1.0, 3.0 and 6.0 g kg(-1). results showed that prawns fed with a diet containing bpe at the level of 1.0, 3.0 and 6.0 g kg(-1) for 120 days had a significantly higher survival rate (30.0%, 40.0% and 56.7%, respectively) than those fed w ...201526118934
dietary supplement of banana (musa acuminata) peels hot-water extract to enhance the growth, anti-hypothermal stress, immunity and disease resistance of the giant freshwater prawn, macrobrachium rosenbergii.in the present study, macrobrachium rosenbergii were fed with diets containing extracts of banana, musa acuminate, fruit's peel (banana peels extract, bpe) at 0, 1.0, 3.0 and 6.0 g kg(-1). the non-specific immune parameters, disease resistance and anti-hypothermal stress were evaluated at 2, 4, 8, 16 and 32 days of post feeding. also, we demonstrated the percent weight gain (pwg), percent length gain (plg), feeding efficiency (fe), and survival rate of giant freshwater prawn at 30, 60, 90, and 1 ...201525634258
lectins with anti-hiv activity: a review.lectins including flowering plant lectins, algal lectins, cyanobacterial lectins, actinomycete lectin, worm lectins, and the nonpeptidic lectin mimics pradimicins and benanomicins, exhibit anti-hiv activity. the anti-hiv plant lectins include artocarpus heterophyllus (jacalin) lectin, concanavalin a, galanthus nivalis (snowdrop) agglutinin-related lectins, musa acuminata (banana) lectin, myrianthus holstii lectin, narcissus pseudonarcissus lectin, and urtica diocia agglutinin. the anti-hiv algal ...201525569520
genome-wide survey of the seagrass zostera muelleri suggests modification of the ethylene signalling network.seagrasses are flowering plants which grow fully submerged in the marine environment. they have evolved a range of adaptations to environmental challenges including light attenuation through water, the physical stress of wave action and tidal currents, high concentrations of salt, oxygen deficiency in marine sediment, and water-borne pollination. although, seagrasses are a key stone species of the costal ecosystems, many questions regarding seagrass biology and evolution remain unanswered. genom ...201525563969
high efficiency transformation of banana [musa acuminata l. cv. matti (aa)] for enhanced tolerance to salt and drought stress through overexpression of a peanut salinity-induced pathogenesis-related class 10 protein.bananas and plantains (musa spp. l.) are important subsistence crops and premium export commodity in several countries, and susceptible to a wide range of environmental and biotic stress conditions. here, we report efficient, rapid, and reproducible agrobacterium-mediated transformation and regeneration of an indian niche cultivar of banana [m. acuminata cv. matti (aa)]. apical meristem-derived highly proliferative multiple shoot clump (msc) explants were transformed with the agrobacterium strai ...201525173686
integrated syntenic and phylogenomic analyses reveal an ancient genome duplication in monocots.unraveling widespread polyploidy events throughout plant evolution is a necessity for inferring the impacts of whole-genome duplication (wgd) on speciation, functional innovations, and to guide identification of true orthologs in divergent taxa. here, we employed an integrated syntenic and phylogenomic analyses to reveal an ancient wgd that shaped the genomes of all commelinid monocots, including grasses, bromeliads, bananas (musa acuminata), ginger, palms, and other plants of fundamental, agric ...201425082857
genomic analysis of nac transcription factors in banana (musa acuminata) and definition of nac orthologous groups for monocots and dicots.identifying the molecular mechanisms underlying tolerance to abiotic stresses is important in crop breeding. a comprehensive understanding of the gene families associated with drought tolerance is therefore highly relevant. nac transcription factors form a large plant-specific gene family involved in the regulation of tissue development and responses to biotic and abiotic stresses. the main goal of this study was to set up a framework of orthologous groups determined by an expert sequence compar ...201424570169
effects of hot-water extract of banana (musa acuminata) fruit's peel on the antibacterial activity, and anti-hypothermal stress, immune responses and disease resistance of the giant freshwater prawn, macrobrachium rosenbegii.the hot-extracts isolated from fruit's peel of banana, musa acuminata, was evaluated on the antibacterial activity to pathogens from aquatic animals, and immunostimulating potential, disease resistance and anti-hypothermal stress in giant freshwater prawn, macrobrachium rosenbergii through injection administration. the banana peel extract (bpe) showed good activity against 1 gram-positive and 3 gram-negative pathogens, including lactococcus garvieae, photobacteria damsella, vibrio alginolyticus ...201424906123
production of bioethanol using agricultural waste: banana pseudo stem.india is amongst the largest banana (musa acuminata) producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. two fungal strains aspergillus ellipticus and aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse wer ...201425477922
trace metals in vegetables and fruits cultivated around the surroundings of tummalapalle uranium mining site, andhra pradesh, india.vegetables (tomato - solanum lycopersicum, green chilli - capsicum annum and bitter gourd - momordica charantia) and fruits (banana - musa acuminata colla, papaya - carica papaya and mosambi - citrus limetta) from the cultivated areas around the tummalapalle uranium mining site were analyzed for trace metals (al, cr, mn, fe, ni, cu, zn, pb, be, v, co, cd and u) using inductively coupled plasma-mass spectrometer (icp-ms). as per the estimated data, the concentrations of trace metals in vegetables ...201428962264
isolation and characterization of three cassava elongation factor 1 alpha (meef1a) promoters.in plant genetic engineering, the identification of gene promoters leading to particular expression patterns is crucial for the development of new genetically modified plant generations. this research was conducted in order to isolate and characterize several new promoters from cassava (manihot esculenta crantz) elongation factor 1 alpha (ef1a) gene family.three promoters meef1a3, meef1a5 and meef1a6 were successfully isolated [corrected]. sequence analyses showed that all of the promoters conta ...201424404183
genome-wide identification, classification and expression analysis of the heat shock transcription factor family in chinese cabbage.the hsf gene family, one of the most important transcription factor families, plays crucial roles in regulating heat resistance. however, a systematic and comprehensive analysis of this gene family has not been reported in chinese cabbage. therefore, systematic analysis of the hsf gene family in chinese cabbage has profound significance. in this study, 35 brhsf genes were identified from chinese cabbage, which could be classified into three groups according to their structural characteristics an ...201424609322
the diversification and activity of hat transposons in musa genomes.sequencing of plant genomes often identified the hat superfamily as the largest group of dna transposons. nevertheless, detailed information on the diversity, abundance and chromosomal localization of plant hat families are rare. by in silico analyses of the reference genome assembly and bacterial artificial chromosome (bac) sequences, respectively, we performed the classification and molecular characterization of hat transposon families in musa acuminata. musa hat transposons are organized in t ...201425377178
enhanced yield of phenolic extracts from banana peels (musa acuminata colla aaa) and cinnamon barks (cinnamomum varum) and their antioxidative potentials in fish oil.the bioactive compounds of banana peels and cinnamon barks were extracted by vacuum microwave and ultrasonic-assisted extraction methods at pre-determined temperatures and times. these methods enhance the yield extracts in shorter time. the highest yields of both extracts were obtained from the conditions which employed the highest temperature and the longest time. the extracts' yield from cinnamon bark method was higher by ultrasonic than vacuum microwave method, while vacuum microwave method g ...201425328205
tissue cell assisted fabrication of tubular catalytic platinum microengines.we report a facile platform for mass production of robust self-propelled tubular microengines. tissue cells extracted from fruits of banana and apple, musa acuminata and malus domestica, are used as the support on which a thin platinum film is deposited by means of physical vapor deposition. upon sonication of the cells/pt-coated substrate in water, microscrolls of highly uniform sizes are spontaneously formed. tubular microengines fabricated with the fruit cell assisted method exhibit a fast mo ...201425143056
inhibition of the recombinant cattle tick rhipicephalus (boophilus) annulatus glutathione s-transferase.rhipicephalus (boophilus) annulatus is a bloodsucking ectoparasite that causes severe production losses in the cattle industry. this study aims to evaluate the in vitro effects of tannic acid, hematin (gst inhibitors) and different plant extracts (rich in tannic acid) on the activity of the recombinant glutathione s-transferase enzyme of the egyptian cattle tick r. annulatus (rragst), in order to confirm their ability to inhibit the parasitic essential detoxification enzyme glutathione s-transfe ...201424953796
genome-wide analysis of repeat diversity across the family musaceae.the banana family (musaceae) includes genetically a diverse group of species and their diploid and polyploid hybrids that are widely cultivated in the tropics. in spite of their socio-economic importance, the knowledge of musaceae genomes is basically limited to draft genome assemblies of two species, musa acuminata and m. balbisiana. here we aimed to complement this information by analyzing repetitive genome fractions of six species selected to represent various phylogenetic groups within the f ...201424932725
evasion of short interfering rna-directed antiviral silencing in musa acuminata persistently infected with six distinct banana streak pararetroviruses.vegetatively propagated crop plants often suffer from infections with persistent rna and dna viruses. such viruses appear to evade the plant defenses that normally restrict viral replication and spread. the major antiviral defense mechanism is based on rna silencing generating viral short interfering rnas (sirnas) that can potentially repress viral genes posttranscriptionally through rna cleavage and transcriptionally through dna cytosine methylation. here we examined the rna silencing machinery ...201425056897
hormonal and hydroxycinnamic acids profiles in banana leaves in response to various periods of water stress.the pattern of change in the endogenous levels of several plant hormones and hydroxycinnamic acids in addition to growth and photosynthetic performance was investigated in banana plants (musa acuminata cv. "grand nain") subjected to various cycles of drought. water stress was imposed by withholding irrigation for six periods with subsequent rehydration. data showed an increase in abscisic acid (aba) and indole-3-acetic acid (iaa) levels, a transient increase in salicylic acid (sa) concentration, ...201424977208
protocol for simultaneous isolation of three important banana allergens.banana fruit (musa acuminata) has become an important food allergen source in recent years. so far, 5 ige reactive banana proteins have been identified, and the major allergens are: mus a 2 (a class i chitinase, 31kda), mus a 4 (thaumatin-like protein, 21kda), and mus a 5 (β-1,3-glucanase, 33kda). due to variations in allergen expression levels, diagnostic reagents for food allergy can be improved by using individual allergen components instead of banana allergen extracts. the purpose of this st ...201424887593
lipophilic phytochemicals from banana fruits of several musa species.the chemical composition of the lipophilic extract of ripe pulp of banana fruit from several banana cultivars belonging to the musa acuminata and musa balbisiana species (namely 'chinese cavendish', 'giant cavendish', 'dwarf red', 'grand nain', 'eilon', 'gruesa', 'silver', 'ricasa', 'williams' and 'zelig') was studied by gas chromatography-mass spectrometry for the first time. the banana cultivars showed similar amounts of lipophilic extractives (ca. 0.4% of dry material weight) as well as quali ...201424874383
expansion of banana (musa acuminata) gene families involved in ethylene biosynthesis and signalling after lineage-specific whole-genome duplications.whole-genome duplications (wgds) are widespread in plants, and three lineage-specific wgds occurred in the banana (musa acuminata) genome. here, we analysed the impact of wgds on the evolution of banana gene families involved in ethylene biosynthesis and signalling, a key pathway for banana fruit ripening. banana ethylene pathway genes were identified using comparative genomics approaches and their duplication modes and expression profiles were analysed. seven out of 10 banana ethylene gene fami ...201424716518
in vitro propagation and assessment of the genetic fidelity of musa acuminata (aaa) cv. vaibalhla derived from immature male flowers.an efficient in vitro propagation method has been developed for the first time for musa acuminata (aaa) cv. vaibalhla, an economically important banana cultivar of mizoram, india. immature male flowers were used as explants. murashige and skoog's (ms) medium supplemented with plant growth regulators (pgrs) were used for the regeneration process. out of different pgr combinations, ms medium supplemented with 2 mg l(-1) 6-benzylaminopurine (bap) + 0.5 mg l(-1) α-naphthalene acetic acid (naa) was o ...201424222501
from crossbreeding to biotechnology-facilitated improvement of banana and plantain.the annual harvest of banana and plantain (musa spp.) is approximately 145 million tons worldwide. about 85% of this global production comes from small plots and kitchen or backyard gardens from the developing world, and only 15% goes to the export trade. musa acuminata and musa balbisiana are the ancestors of several hundreds of parthenocarpic musa diploid and polyploid cultivars, which show multiple origins through inter- and intra-specific hybridizations from these two wild diploid species. g ...201424091289
annotation of differentially expressed genes in the somatic embryogenesis of musa and their location in the banana genome.analysis of cdna-aflp was used to study the genes expressed in zygotic and somatic embryogenesis of musa acuminata colla ssp. malaccensis, and a comparison was made between their differential transcribed fragments (tdfs) and the sequenced genome of the double haploid- (dh-) pahang of the malaccensis subspecies that is available in the network. a total of 253 transcript-derived fragments (tdfs) were detected with apparent size of 100-4000 bp using 5 pairs of aflp primers, of which 21 were differe ...201324027442
chlorophyll breakdown in senescent banana leaves: catabolism reprogrammed for biosynthesis of persistent blue fluorescent tetrapyrroles.chlorophyll breakdown is a visual phenomenon of leaf senescence and fruit ripening. it leads to the formation of colorless chlorophyll catabolites, a group of (chlorophyll-derived bilin-type) linear tetrapyrroles. here, analysis and structure elucidation of the chlorophyll breakdown products in leaves of banana (musa acuminata) is reported. in senescent leaves of this monocot all chlorophyll catabolites identified were hypermodified fluorescent chlorophyll catabolites (hmfccs). surprisingly, non ...201323946204
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