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functional characterization of naturally occurring wild soybean mutant (sg-5) lacking astringent saponins using whole genome sequencing approach.triterpenoid saponins are one of the most highly accumulated groups of functional components in soybean (glycine max) and the oxidative reactions during their biosynthesis are required for their aglycone diversity. natural mutants of soyasaponins in wild soybean (glycine soja) are valuable resources for establishing the soyasaponin biosynthesis pathway and breeding new soybean varieties. in this study, we investigated the genetic mechanism behind the absence of group a saponins in a korean wild ...201829362093
elevation of soybean seed oil content through selection for seed coat shininess.many leguminous species have adapted their seed coat with a layer of powdery bloom that contains hazardous allergens and makes the seeds less visible, offering duel protection against potential predators 1 . nevertheless, a shiny seed surface without bloom is desirable for human consumption and health, and is targeted for selection under domestication. here we show that seed coat bloom in wild soybeans is mainly controlled by bloom1 (b1), which encodes a transmembrane transporter-like protein fo ...201829292374
adaptive evolution of rhizobial symbiotic compatibility mediated by co-evolved insertion sequences.mutualism between bacteria and eukaryotes has essential roles in the history of life, but the evolution of their compatibility is poorly understood. here we show that different sinorhizobium strains can form either nitrogen-fixing nodules or uninfected pseudonodules on certain cultivated soybeans, while being all effective microsymbionts of some wild soybeans. however, a few well-infected nodules can be found on a commercial soybean using inocula containing a mixed pool of tn5 insertion mutants ...201828800133
comparative analysis of complete plastid genomes from wild soybean (glycine soja) and nine other glycine species.the plastid genomes of different plant species exhibit significant variation, thereby providing valuable markers for exploring evolutionary relationships and population genetics. glycine soja (wild soybean) is recognized as the wild ancestor of cultivated soybean (g. max), representing a valuable genetic resource for soybean breeding programmes. in the present study, the complete plastid genome of g. soja was sequenced using illumina paired-end sequencing and then compared it for the first time ...201728763486
the difference of photosynthetic responses to the cadmium stress between a wild soybean (glycine soja sieb. et zucc.) and a cultivated soybean.the effects of cd on a halophyte of doingying wild soybean (dy-03262) were studied by comparing it with a cultivated soybean (sn-11) via investigating photosynthetic activity, chlorophyll fluorescence and cd concentrations in leaves after treatment with different concentrations of cd (0, 50, and 100 μm) for 10 days. there were no significant differences in photosynthetic rate (pn), actual photochemical efficiency of psii (φpsii), stomatal conductance (gs), and photochemical quenching (qp) betwee ...201728752180
mapping novel aphid resistance qtl from wild soybean, glycine soja 85-32.two novel qtls conferring aphid resistance were mapped and validated on soybean chromosomes 8 and 16, respectively. closely linked markers were developed to assist breeding for aphid resistance. soybean aphid, aphis glycines matsumura, is a highly destructive pest for soybean production. e08934, a soybean advanced breeding line derived from the wild soybean glycine soja 85-32, has shown strong resistance to aphids. to dissect the genetic basis of aphid resistance in e08934, a mapping population ...201728710504
comparison of salt tolerance in soja based on metabolomics of seedling roots.soybean is an important economic crop that is continually threatened by abiotic stresses, especially salt stress. wild soybean is an important germplasm resource for the breeding of cultivated soybean. the root system plays a very important role in plant salt tolerance. to explore the salt tolerance-related mechanisms among soja, we have demonstrated the seedling roots' growth and metabolomics in wild soybean, semi-wild soybean, and cultivated soybean under two types of salt stress by using gas ...201728690628
a novel ap2/erf family transcription factor from glycine soja, gserf71, is a dna binding protein that positively regulates alkaline stress tolerance in arabidopsis.here we first found that gserf71, an erf factor from wild soybean could increase plant alkaline stress tolerance by up-regulating h+-atpase and by modifing the accumulation of auxin. alkaline soils are widely distributed all over the world and greatly limit plant growth and development. in our previous transcriptome analyses, we have identified several erf (ethylene-responsive factor) genes that responded strongly to bicarbonate stress in the roots of wild soybean g07256 (glycine soja). in this ...201728681139
seed weight differences between wild and domesticated soybeans are associated with specific changes in gene expression.our study systematically explored potential genes and molecular pathways as candidates for differences in seed weight resulting from soybean domestication. in addition, potential contributions of lncrnas to seed weight were also investigated. soybeans have a long history of domestication in china, and there are several significant phenotypic differences between cultivated and wild soybeans, for example, seeds of cultivars are generally larger and heavier than those from wild accessions. we analy ...201728653111
extraction optimization, preliminary characterization and antioxidant activities of polysaccharides from glycine soja.single-factor experiment and central composite design (ccd) was applied to optimize the ultrasound-assisted extraction (uae) conditions of polysaccharides from glycine soja (cgps), and a preliminary characterization of three polysaccharide fractions (cgps, gps-1, and gps-2) and their antioxidant activities were investigated. under the optimal conditions: ratio of liquid to solid 42.7ml/g, extraction power 293.7w, extraction temperature 68.9°c, and extraction time 34.7min, the experimental cgps y ...201728579464
gsslah3, a glycine soja slow type anion channel homologue, positively modulates plant bicarbonate stress tolerance.alkaline stress is a major form of abiotic stress that severely inhibits plant growth and development, thus restricting crop productivity. however, little is known about how plants respond to alkali. in this study, a slow-type anion channel homologue 3 gene gsslah3 was isolated and functionally characterized. bioinformatics analysis showed that gsslah3 protein contains ten transmembrane helices. consistently, gsslah3 was found to locate on plasma membrane by transient expression in onion epiderm ...201729243826
genome-wide analysis and expression profiling of pp2c clade d under saline and alkali stresses in wild soybean and arabidopsis.protein phosphatase 2cs (pp2cs) belong to the largest protein phosphatase family in plants. some members have been described as being negative modulators of plant growth and development, as well as responses to hormones and environmental stimuli. however, little is known about the members of pp2c clade d, which may be involved in the regulation of signaling pathways, especially in response to saline and alkali stresses. here, we identified 13 pp2c orthologs from the wild soybean (glycine soja) g ...201729052008
differences in the metabolic profiles and antioxidant activities of wild and cultivated black soybeans evaluated by correlation analysis.wild soybeans are considered a potential resource for soybean domestication and an important source of genetic diversity for soybean crop improvement. understanding metabolite-caused bioactivity differences between cultivated and wild soybeans is essential for designing a soybean with enhanced nutritional traits. in this study, the non-targeted metabolic profiling of 26 soybean varieties, 15 wild black soybeans (wbs) and 11 cultivated black soybeans (cbs), using liquid chromatography-mass spectr ...201728888437
fine mapping of the soybean aphid-resistance genes rag6 and rag3c from glycine soja 85-32.rag6 and rag3c were delimited to a 49-kb interval on chromosome 8 and a 150-kb interval on chromosome 16, respectively. structural variants in the exons of candidate genes were identified. the soybean aphid, an invasive species, has significantly threatened soybean production in north america since 2000. host-plant resistance is known as an ideal management strategy for aphids. two novel aphid-resistance loci, rag6 and rag3c, from glycine soja 85-32, were previously detected in a 10.5-cm interva ...201728887657
the glycine soja nac transcription factor gsnac019 mediates the regulation of plant alkaline tolerance and aba sensitivity.overexpression of gshdz4 or gsnac019 enhanced alkaline tolerance in transgenic arabidopsis. we proved that gshdz4 up-regulated both gsnac019 and gsrd29b but gsnac019 may repress the gsrd29b expression under alkaline stress. wild soybean (glycine soja) has a high tolerance to environmental challenges. it is a model species for dissecting the molecular mechanisms of salt-alkaline stresses. although many nac transcription factors play important roles in response to multiple abiotic stresses, such a ...201728884328
gschx19.3, a member of cation/h+ exchanger superfamily from wild soybean contributes to high salinity and carbonate alkaline tolerance.cation/h+ exchangers (chx) are characterized to be involved in plant growth, development and stress responses. although soybean genome sequencing has been completed, the chx family hasn't yet been systematically analyzed, especially in wild soybean. here, through hidden markov model search against glycine soja proteome, 34 gschxs were identified and phylogenetically clustered into five groups. members within each group showed high conservation in motif architecture. interestingly, according to o ...201728842677
gssnap33, a novel glycine soja snap25-type protein gene: improvement of plant salt and drought tolerances in transgenic arabidopsis thaliana.the n-ethylmaleimide-sensitive factor attachment protein receptor (snare) superfamily, specifically the snap25-type proteins and t-snares, have been proposed to regulate cellular processes and plant resistance mechanisms. however, little is known about the role of snap25-type proteins in combating abiotic stresses, specifically in wild soybean. in the current study, the isolation and functional characterization of the putative synaptosomal-associated snap25-type protein gene gssnap33 from wild s ...201728841544
qtl mapping of antixenosis resistance to common cutworm (spodoptera litura fabricius) in wild soybean (glycine soja).the common cutworm (ccw; spodoptera litura fabricius) is a serious herbivorous insect pest of soybean (glycine max) in asia and oceania. previously, we identified quantitative trait loci (qtls) for ccw-antibiosis-resistance, ccw-1 and ccw-2, and antixenosis-resistance, qrslx1 and qrslx2, in the cultivar 'himeshirazu'. the effects of these qtls are useful in the breeding of ccw-resistant cultivars. in this study, we conducted an antixenosis bioassay on ccw using recombinant inbred lines derived f ...201729232719
chromosome segment detection for seed size and shape traits using an improved population of wild soybean chromosome segment substitution lines.size and shape of soybean seeds are closely related to seed yield and market value. annual wild soybeans have the potential to improve cultivated soybeans, but their inferior seed characteristics should be excluded. to detect quantitative trait loci (qtls)/segments of seed size and shape traits in annual wild soybean, its chromosome segment substitution lines (cssls) derived from nn1138-2 (recurrent parent, glycine max) and n24852 (donor parent, glycine soja) and then modified 2 iterations (code ...201729158636
soybean mosaic virus: a successful potyvirus with a wide distribution but restricted natural host range.taxonomy. soybean mosaic virus (smv) is a species within the genus potyvirus, family potyviridae that includes almost a quarter of all known plant rna viruses affecting agriculturally important plants. the potyvirus genus is the largest of all genera of plant rna viruses with 160 species. particle. the filamentous particles of smv, typical of potyviruses, are about 7,500 å long and 120 å in diameter with a central hole of about 15 å in diameter. coat protein residues are arranged in helice of ab ...201729134790
likelihood assessment for gene flow of transgenes from imported genetically modified soybean (glycine max (l.) merr.) to wild soybean (glycine soja seib. et zucc.) in japan as a component of environmental risk assessment.environmental risk assessment is required for genetically modified (gm) crops before their import into japan. annual roadside monitoring along transportation routes from ports to processing facilities for gm soybean (glycine max (l.) merr.) have been requested as a condition of import only approval because of lack of information on the likelihood of persistence of imported gm soybean for food, feed and processing and the potential for transfer of transgenes into wild soybean (glycine soja seib. ...201729085244
molecular cloning, recombinant expression and antifungal activity of bncpi, a cystatin in ramie (boehmeria nivea l.).phytocystatins play multiple roles in plant growth, development and resistance to pests and other environmental stresses. a ramie (boehmeria nivea l.) phytocystatin gene, designated as bncpi, was isolated from a ramie cdna library and its full-length cdna was obtained by rapid amplification of cdna ends (race). the full-length cdna sequence (691 bp) consisted of a 303 bp open reading frame (orf) encoding a protein of 100 amino acids with deduced molecular mass of 11.06 kda and a theoretical isoe ...201729019965
transcriptomic variation in proanthocyanidin biosynthesis pathway genes in soybean (glycine spp.).proanthocyanidins are oligomeric or polymeric end products of flavonoid metabolic pathways starting with the central phenylpropanoid pathway. although soybean (glycine spp.) seeds represent a major source of nutrients for the human diet, as well as components for the cosmetics industry as a result of their high levels of flavonoid metabolites, including isoflavonoids, anthocyanins and proanthocyanidins, the genetic regulatory mechanisms underlying proanthocyanidin biosynthesis in soybean remain ...201728960323
soybean aphid, aphis glycines (hemiptera: aphididae), developmental and reproductive capacity on white clover, trifolium repens (rosales: leguminosae), in northeast china.nymphs of aphis glycines matsumura were individually reared to adults in the laboratory on detached leaf discs of trifolium repens l. (white clover) mounted on agar medium. adults of a. glycines were fed t. repens within small clip cages in the field. development, reproduction and intrinsic rates of increase of a. glycines were studied. these data were compared to those of controls fed known host plants including cultivated soybean glycine max (l.) merr. and the wild soybean species glycine soja ...201728798493
impact of seed protein alleles from three soybean sources on seed composition and agronomic traits.evaluation of seed protein alleles in soybean populations showed that an increase in protein concentration is generally associated with a decrease in oil concentration and yield. soybean [glycine max (l.) merrill] meal is one of the most important plant-based protein sources in the world. developing cultivars high in seed protein concentration and seed yield is a difficult task because the traits have an inverse relationship. over two decades ago, a protein quantitative trait loci (qtl) was mapp ...201728795235
rna-seq study reveals genetic responses of diverse wild soybean accessions to increased ozone levels.ozone is an air pollutant widely known to cause a decrease in productivity in many plant species, including soybean (glycine max (l.) merr). while the response of cultivated soybean to ozone has been studied, very little information is available regarding the ozone response of its wild relatives.201728662633
changes in gene expression between a soybean f1 hybrid and its parents are associated with agronomically valuable traits.soybean [glycine max (l.) merr.] genetic diversity is limited because domesticated soybean has undergone multiple genetic bottlenecks. its progenitor, the wild soybean [glycine soja siebold & zucc], has not undergone the same intense selection and is much more genetically diverse than domesticated soybean. however, the agronomic importance of diversity in wild soybean is unclear, and its weedy nature makes assessment difficult. to address this issue, we chose for study a highly selected, adapted ...201728493991
identity recognition in response to different levels of genetic relatedness in commercial soya bean.identity recognition systems allow plants to tailor competitive phenotypes in response to the genetic relatedness of neighbours. there is limited evidence for the existence of recognition systems in crop species and whether they operate at a level that would allow for identification of different degrees of relatedness. here, we test the responses of commercial soya bean cultivars to neighbours of varying genetic relatedness consisting of other commercial cultivars (intraspecific), its wild proge ...201728280587
functional analysis of flower development related gene gslfy from glycine soja.leafy/floricaula (lfy/flo) is a family of plant-specific transcription factors, which plays an important role(s) in the regulation of floral organ formation and development. so far, lfy regulation on floral development in wild soybean has not been reported in the literature. in this study, the lfy gene, gslfy, has been isolated from glycine soja, and characterized with molecular and transgenic techniques. the cdna for gslfy gene is 1224 bp in length and contains an open reading frame encoding a ...201728115306
ensifer shofinae sp. nov., a novel rhizobial species isolated from root nodules of soybean (glycine max).two bacterial strains isolated from root nodules of soybean were characterized phylogenetically as members of a distinct group in the genus ensifer based on 16s rrna gene comparisons. they were also verified as a separated group by the concatenated sequence analyses of reca, atpd and glnii (with similarities ≤93.9% to the type strains for defined species), and by the average nucleotide identities (ani) between the whole genome sequence of the representative strain ccbau 251167(t) and those of th ...201728209394
selection for a zinc-finger protein contributes to seed oil increase during soybean domestication.seed oil is a momentous agronomical trait of soybean (glycine max) targeted by domestication in breeding. although multiple oil-related genes have been uncovered, knowledge of the regulatory mechanism of seed oil biosynthesis is currently limited. we demonstrate that the seed-preferred gene gmzf351, encoding a tandem ccch zinc finger protein, is selected during domestication. further analysis shows that gmzf351 facilitates oil accumulation by directly activating wrinkled1, biotin carboxyl carrie ...201728184009
complete nucleotide sequences of seven soybean mosaic viruses (smv), isolated from wild soybeans (glycine soja) in china.soybean mosaic virus (smv) is a devastating plant virus classified in the family potyviridae, and known to infect cultivated soybeans (glycine max). in this study, seven new smvs were isolated from wild soybean samples and analyzed by whole-genome sequencing. an updated smv phylogeny was built with the seven new and 83 known smv genomic sequences. results showed that three northeastern smv isolates were distributed in clade iii and iv, while four southern smvs were grouped together in clade ii a ...201727909932
physiological responses of three soybean species (glycine soja, g. gracilis, and g. max cv. melrose) to salinity stress.understanding the mechanism for salt tolerance in wild soybean (glycine soja) can help researchers improve that trait in cultivated soybean lines. we analyzed the effects of excess nacl on the growth, physiology, and ion distribution in three soybean species: wild g. soja (w8), semi-wild g. gracilis (sw18), and the cultivated salt-sensitive g. max (cv. melrose). these comparisons revealed that, under salt stress, shoot and root lengths and biomass (either shoot or root dry weights) were signific ...201728378100
a pp2c-1 allele underlying a quantitative trait locus enhances soybean 100-seed weight.cultivated soybeans may lose some useful genetic locus during domestication. introgression of genes from wild soybeans may broaden the genetic background and improve soybean agronomic traits. here, through whole-genome sequencing of an ril population derived from a cross between a wild soybean zyd7 and a cultivated soybean hn44, and mapping of qtls for seed weight, we discover that a phosphatase 2c-1 (pp2c-1) allele from wild soybean zyd7 contributes to the increase of seed weight/size in transg ...201728363587
plasticity and innovation of regulatory mechanisms underlying seed oil content mediated by duplicated genes in the palaeopolyploid soybean.many plants have undergone whole genome duplication (wgd). however, how regulatory networks underlying a particular trait are reshaped in polyploids has not been experimentally investigated. here we show that the regulatory pathways modulating seed oil content, which involve wrinkled1 (wri1), leafy cotyledon1 (lec1), and lec2 in arabidopsis, have been modified in the palaeopolyploid soybean. such modifications include functional reduction of gmwri1b of the gmwri1a/gmwri1b homoeologous pair relev ...201728295817
evolutionary trajectories of duplicated ft homologues and their roles in soybean domestication.to clarify the molecular bases of flowering time evolution in crop domestication, here we investigate the evolutionary fates of a set of four recently duplicated genes in soybean: ft2a, ft2b, ft2c and ft2d that are homologues of the floral inducer flowering locus t (ft). while ft2a maintained the flowering inducer function, other genes went through contrasting evolutionary paths. ft2b evolved attenuated expression potentially associated with a transposon insertion in the upstream intergenic regi ...201728244155
erratum to: complete nucleotide sequences of seven soybean mosaic viruses (smv), isolated from wild soybeans (glycine soja) in china. 201728116525
a genome-wide association study of seed composition traits in wild soybean (glycine soja).cultivated soybean (glycine max) is a major agricultural crop that provides a crucial source of edible protein and oil. decreased amounts of saturated palmitic acid and increased amounts of unsaturated oleic acid in soybean oil are considered optimal for human cardiovascular health and therefore there has considerable interest by breeders in discovering genes affecting the relative concentrations of these fatty acids. using a genome-wide association (gwa) approach with nearly 30,000 single nucle ...201728056769
rna-seq data comparisons of wild soybean genotypes in response to soybean cyst nematode (heterodera glycines).soybean [glycine max (l.) merr.] is an important crop rich in vegetable protein and oil, and is a staple food for human and animals worldwide. however, soybean plants have been challenged by soybean cyst nematode (scn, heterodera glycines), one of the most damaging pests found in soybean fields. applying scn-resistant cultivars is the most efficient and environmentally friendly strategy to manage scn. currently, soybean breeding and further improvement in soybean agriculture are hindered by seve ...201728856099
comparative rna-seq analysis uncovers a complex regulatory network for soybean cyst nematode resistance in wild soybean (glycine soja).soybean cyst nematode (scn) is the most damaging pest of soybean worldwide. the molecular mechanism of scn resistance remains largely unknown. we conducted a global rna-seq comparison between a resistant genotype (s54) and a susceptible genotype (s67) of glycine soja, the wild progenitor of soybean, to understand its regulatory network in scn defense. the number of differentially expressed genes (degs) in s54 (2,290) was much larger than that in s67 (555). a number of defense-related genes/pathw ...201728852059
genetic architecture of wild soybean (glycine soja) response to soybean cyst nematode (heterodera glycines).the soybean cyst nematode (scn) is one of the most destructive pathogens of soybean plants worldwide. host-plant resistance is an environmentally friendly method to mitigate scn damage. to date, the resistant soybean cultivars harbor limited genetic variation, and some are losing resistance. thus, a better understanding of the genetic mechanisms of the scn resistance, as well as developing diverse resistant soybean cultivars, is urgently needed. in this study, a genome-wide association study (gw ...201728710561
genome-wide identification of major intrinsic proteins in glycine soja and characterization of gmtip2;1 function under salt and water stress.in different plant species, aquaporins (aqps) facilitate water movement by regulating root hydraulic conductivity under diverse stress conditions such as salt and water stresses. to improve survival and yield of crop plants, a detailed understanding of stress responses is imperative and required. we used glycine soja genome as a tool to study aqps, considering it shows abundant genetic diversity and higher salt environment tolerance features and identified 62 gs aqp genes. additionally, this stu ...201728646139
hematologic complications in a patient with glycine soja polyagglutination following fresh frozen plasma transfusion.polyagglutination is a rare and underdiagnosed condition, characterized by agglutination of red blood cells(rbcs) with almost all abo-compatible adult sera. polyagglutination can occur when a cryptantigen is exposed on rbcs via microbial enzyme activity. becausenearly all adults naturally produce antibodies against cryptantigens, transfusion of plasma can cause unexpected hemolysis and hematologic complications, such as thrombocytopenia and disseminated intravascular coagulation, in patients who ...201728657762
comparison of small rna profiles of glycine max and glycine soja at early developmental stages.small rnas, including micrornas (mirnas) and phased small interfering rnas (phasirnas; from phas loci), play key roles in plant development. cultivated soybean, glycine max, contributes a great deal to food production, but, compared to its wild kin, glycine soja, it may lose some genetic information during domestication. in this work, we analyzed the srna profiles of different tissues in both cultivated (c08) and wild soybeans (w05) at three stages of development. a total of 443 known mirnas and ...201627929436
natural diversity of seed α-tocopherol ratio in wild soybean (glycine soja) germplasm collection.alpha-tocopherol is one of four tocopherol isoforms and has the highest vitamin e activity in humans. most cultivated soybean seeds contain γ-tocopherol as the predominant form, and the ratio of α-tocopherol content to total tocopherol content (α-tocopherol ratio) is <10%. three soybean accessions from eastern europe have α-tocopherol ratios of >20%. this higher content is likely due to mutations in the promoter region of the γ-tocopherol methytransferase-3 (γ-tmt3) gene. we surveyed a wild soyb ...201627795691
environmental versus geographical effects on genomic variation in wild soybean (glycine soja) across its native range in northeast asia.a fundamental goal in evolutionary biology is to understand how various evolutionary factors interact to affect the population structure of diverse species, especially those of ecological and/or agricultural importance such as wild soybean (glycine soja). g. soja, from which domesticated soybeans (glycine max) were derived, is widely distributed throughout diverse habitats in east asia (russia, japan, korea, and china). here, we utilize over 39,000 single nucleotide polymorphisms genotyped in 99 ...201627648247
characterizing two inter-specific bin maps for the exploration of the qtls/genes that confer three soybean evolutionary traits.annual wild soybean (glycine soja sieb. and zucc.), the wild progenitor of the cultivated soybean [glycine max (l.) merr.], is valuable for improving the later. the construction of a linkage map is crucial for studying the genetic differentiation between these species, but marker density is the main factor limiting the accuracy of such a map. recent advances in next-generation sequencing technologies allow for the generation of high-density linkage maps. here, two sets of inter-specific recombin ...201627602037
metabolic profiles reveal changes in wild and cultivated soybean seedling leaves under salt stress.clarification of the metabolic mechanisms underlying salt stress responses in plants will allow further optimization of crop breeding and cultivation to obtain high yields in saline-alkali land. here, we characterized 68 differential metabolites of cultivated soybean (glycine max) and wild soybean (glycine soja) under neutral-salt and alkali-salt stresses using gas chromatography-mass spectrometry (gc-ms)-based metabolomics, to reveal the physiological and molecular differences in salt tolerance ...201627442489
identification and molecular analysis of four new alleles at the w1 locus associated with flower color in soybean.in soybean, flavonoid 3'5'-hydroxylase (f3'5'h) and dihydroflavonol-4-reductase (dfr) play a crucial role in the production of anthocyanin pigments. loss-of-function of the w1 locus, which encodes the former, or w3 and w4, which encode the latter, always produces white flowers. in this study, we searched for new genetic components responsible for the production of white flowers in soybean and isolated four white-flowered mutant lines, i.e., two glycine soja accessions (cw12700 and cw13381) and t ...201627442124
optimization of a virus-induced gene silencing system with soybean yellow common mosaic virus for gene function studies in soybeans.virus-induced gene silencing (vigs) is an effective tool for the study of soybean gene function. successful vigs depends on the interaction between virus spread and plant growth, which can be influenced by environmental conditions. recently, we developed a new vigs system derived from the soybean yellow common mosaic virus (sycmv). here, we investigated several environmental and developmental factors to improve the efficiency of a sycmv-based vigs system to optimize the functional analysis of th ...201627147931
molecular and geographic evolutionary support for the essential role of giganteaa in soybean domestication of flowering time.flowering time is a domestication trait of glycine max and varies in soybeans, yet, a gene for flowering time variation has not been associated with soybean domestication. gigantea (gi) is a major gene involved in the control of flowering time in arabidopsis, although three gi homologs complicate this model in the soybean genome.201627072125
the transcriptomic signature of developing soybean seeds reveals the genetic basis of seed trait adaptation during domestication.cultivated soybean has undergone many transformations during domestication. in this paper we report a comprehensive assessment of the evolution of gene co-expression networks based on the analysis of 40 transcriptomes from developing soybean seeds in cultivated and wild soybean accessions. we identified 2680 genes that are differentially expressed during seed maturation and established two cultivar-specific gene co-expression networks. through analysis of the two networks and integration with qu ...201627062090
characterization of natural and simulated herbivory on wild soybean (glycine soja seib. et zucc.) for use in ecological risk assessment of insect protected soybean.insect-protected soybean (glycine max (l.) merr.) was developed to protect against foliage feeding by certain lepidopteran insects. the assessment of potential consequences of transgene introgression from soybean to wild soybean (glycine soja seib. et zucc.) is required as one aspect of the environmental risk assessment (era) in japan. a potential hazard of insect-protected soybean may be hypothesized as transfer of a trait by gene flow to wild soybean and subsequent reduction in foliage feeding ...201626963815
qtl location and epistatic effect analysis of 100-seed weight using wild soybean (glycine soja sieb. & zucc.) chromosome segment substitution lines.increasing the yield of soybean (glycine max l. merrill) is a main aim of soybean breeding. the 100-seed weight is a critical factor for soybean yield. to facilitate genetic analysis of quantitative traits and to improve the accuracy of marker-assisted breeding in soybean, a valuable mapping population consisting of 194 chromosome segment substitution lines (cssls) was developed. in these lines, different chromosomal segments of the chinese cultivar suinong 14 were substituted into the genetic b ...201626934088
evaluation of genetic variation among brazilian soybean cultivars through genome resequencing.soybean [glycine max (l.) merrill] is one of the most important legumes cultivated worldwide, and brazil is one of the main producers of this crop. since the sequencing of its reference genome, interest in structural and allelic variations of cultivated and wild soybean germplasm has grown. to investigate the genetics of the brazilian soybean germplasm, we selected soybean cultivars based on the year of commercialization, geographical region and maturity group and resequenced their genomes.201626872939
development and application of a novel genome-wide snp array reveals domestication history in soybean.domestication of soybeans occurred under the intense human-directed selections aimed at developing high-yielding lines. tracing the domestication history and identifying the genes underlying soybean domestication require further exploration. here, we developed a high-throughput njau 355 k soysnp array and used this array to study the genetic variation patterns in 367 soybean accessions, including 105 wild soybeans and 262 cultivated soybeans. the population genetic analysis suggests that cultiva ...201626856884
environmental association analyses identify candidates for abiotic stress tolerance in glycine soja, the wild progenitor of cultivated soybeans.natural populations across a species range demonstrate population structure owing to neutral processes such as localized origins of mutations and migration limitations. selection also acts on a subset of loci, contributing to local adaptation. an understanding of the genetic basis of adaptation to local environmental conditions is a fundamental goal in basic biological research. when applied to crop wild relatives, this same research provides the opportunity to identify adaptive genetic variatio ...201626818076
global investigation of the co-evolution of mirna genes and microrna targets during soybean domestication.although the selection of coding genes during plant domestication has been well studied, the evolution of mirna genes (mirs) and the interaction between micrornas (mirnas) and their targets in this process are poorly understood. here, we present a genome-wide survey of the selection of mirs and mirna targets during soybean domestication and improvement. our results suggest that, overall, mirs have higher evolutionary rates than mirna targets. nonetheless, they do demonstrate certain similar evol ...201626714457
ensifer glycinis sp. nov., a rhizobial species associated with species of the genus glycine.rhizobial strains from root nodules of astragalus mongholicus and soybean (glycine max) were characterized phylogenetically as members of the genus ensifer (formerly named sinorhizobium), based on 16s rrna gene sequence comparisons. results based upon concatenated sequence analysis of three housekeeping genes (reca, atpd and glnii, ≤ 93.8 % similarities to known species) and average nucleotide identity (ani) values of whole genome sequence comparisons (ranging from 89.6 % to 83.4 % to ensifer fr ...201627125987
improvement in nitrogen fixation capacity could be part of the domestication process in soybean.biological nitrogen fixation (bnf) in soybeans is a complex process involving the interplay between the plant host and the symbiotic rhizobia. as nitrogen supply has a crucial role in growth and development, higher nitrogen fixation capacity would be important to achieve bigger plants and larger seeds, which were important selection criteria during plant domestication by humans. to test this hypothesis, we monitored the nitrogen fixation-related performance in 31 cultivated and 17 wild soybeans ...201627118154
genome-wide association study of resistance to soybean cyst nematode (heterodera glycines) hg type 2.5.7 in wild soybean (glycine soja).soybean cyst nematode (scn) is the most destructive soybean pest worldwide. host plant resistance is the most environmentally friendly and cost-effective way of mitigating scn damage to soybeans. however, overuse of the resistant soybean [glycine max (l.) merr.] cultivars from limited genetic resources has resulted in scn race shifts in many soybean-growing areas. thus, exploration of novel sources of scn resistance and dissection of the genetic basis are urgently needed. in this study, we scree ...201627582748
soybean cyst nematode resistance emerged via artificial selection of duplicated serine hydroxymethyltransferase genes.a major soybean (forrest cultivar) quantitative trait locus (qtl) gene, rhg4, which controls resistance to soybean cyst nematodes (scn), encodes the enzyme serine hydroxylmethyltransferase (shmt). the resistant allele possesses two critical missense mutations (p130r and n358y) compared to that of the sensitive allele, rhg4. to understand the evolutionary history of this gene, sequences of 117 shmt family members from 18 representative plant species were used to reconstruct their phylogeny. accor ...201627458476
genetic diversity of rhg1 and rhg4 loci in wild soybeans resistant to soybean cyst nematode race 3.over-utilization of germplasms that are resistant to the soybean cyst nematode (scn) in soybean breeding programs can lead to genetic vulnerability in resistant cultivars. resistant wild soybean (glycine soja) is considered an invaluable gene source for increasing the genetic diversity of scn resistance. in this study, we genotyped 23 g. soja accessions that are resistant to scn race 3 for polymorphisms in the resistance genes, rhg1, rhg4, and shmt, and investigated their genetic relationship wi ...201627323148
genome-wide association and genomic prediction identifies associated loci and predicts the sensitivity of tobacco ringspot virus in soybean plant introductions.genome-wide association study (gwas) is a useful tool for detecting and characterizing traits of interest including those associated with disease resistance in soybean. the availability of 50,000 single nucleotide polymorphism (snp) markers (soysnp50k iselect beadchip; www.soybase.org ) on 19,652 soybean and wild soybean plant introductions (pis) in the usda soybean germplasm collection allows for fast and robust identification of loci associated with a desired phenotype. by using a genome-wide ...201626924079
soybean gmdrebl increases lipid content in seeds of transgenic arabidopsis.a dreb-type transcription factor gene gmdrebl has been characterized for its functions in oil accumulation in seeds. the gene is specifically expressed in soybean seeds. the gmdrebl is localized in nucleus and has transcriptional activation ability. overexpression of gmdrebl increased the fatty acid content in the seeds of transgenic arabidopsis plants. gmdrebl can bind to the promoter region of wri1 to activate its expression. several other genes in the fatty acid biosynthesis pathway were also ...201627694917
the nuclear protein gmbzip110 has transcription activation activity and plays important roles in the response to salinity stress in soybean.plant basic-leucine zipper (bzip) transcription factors play important roles in many biological processes and are involved in the regulation of salt stress tolerance. previously, our lab generated digital gene expression profiling (dgep) data to identify differentially expressed genes in a salt-tolerant genotype of glycine soja (stgogs) and a salt-sensitive genotype of glycine max (ssgogm). this dgep data revealed that the expression (log2 ratio) of gmbzip110 was up-regulated 2.76-fold and 3.38- ...201626837841
identification and analysis of nahco3 stress responsive genes in wild soybean (glycine soja) roots by rna-seq.soil alkalinity is a major abiotic constraint to crop productivity and quality. wild soybean (glycine soja) is considered to be more stress-tolerant than cultivated soybean (g. max), and has considerable genetic variation for increasing alkalinity tolerance of soybean. in this study, we analyzed the transcriptome profile in the roots of an alkalinity tolerant wild soybean variety n24852 at 12 and 24 h after 90 mm nahco3 stress by rna-sequencing. compared with the controls, a total of 449 differe ...201628018382
structural and functional characterization of the vq protein family and vq protein variants from soybean.proteins containing the fxxxvqxhtg or vq motif interact with wrky transcription factors. although vq proteins have been reported in several plants, knowledge about their structures, functions and evolution is still very limited. here, we report structural and functional analysis of the vq protein family from soybean. like arabidopsis homologues, soybean vq proteins bind only group i and iic wrky proteins and a substantial number of their genes are responsive to stress-associated phytohormones. o ...201627708406
a novel glycine soja homeodomain-leucine zipper (hd-zip) i gene, gshdz4, positively regulates bicarbonate tolerance and responds to osmotic stress in arabidopsis.wild soybean (glycine soja) is a highly adaptive plant species which can grow well in saline-alkaline soils. in soybean genome, there exist about 140 hd-zip (homeodomain-leucine zipper) genes. hd-zip transcription factor family is one of the largest plant specific superfamilies and plays important roles in response to abiotic stresses. although hd-zip transcription factors have been broadly reported to be involved in plant resistance to abiotic stresses like salt and drought, their roles in resp ...201627553065
gserf6, an ethylene-responsive factor from glycine soja, mediates the regulation of plant bicarbonate tolerance in arabidopsis.this is an original study focus on erf gene response to alkaline stress. gserf6 functions as transcription factor and significantly enhanced plant tolerance to bicarbonate (hco 3 (-) ) in transgenic arabidopsis . alkaline stress is one of the most harmful, but little studied environmental factors, which negatively affects plant growth, development and yield. the cause of alkaline stress is mainly due to the damaging consequence of high concentration of the bicarbonate ion, high-ph, and osmotic s ...201627125386
a glycine soja methionine sulfoxide reductase b5a interacts with the ca(2+) /cam-binding kinase gscbrlk and activates ros signaling under carbonate alkaline stress.although research has extensively illustrated the molecular basis of plant responses to salt and high-ph stresses, knowledge on carbonate alkaline stress is poor and the specific responsive mechanism remains elusive. we have previously characterized a glycine soja ca(2+) /cam-dependent kinase gscbrlk that could increase salt tolerance. here, we characterize a methionine sulfoxide reductase (msr) b protein gsmsrb5a as a gscbrlk interactor by using y2h and bifc assays. further analyses showed that ...201627121031
environmental and historical determinants of patterns of genetic differentiation in wild soybean (glycine soja sieb. et zucc).wild soybean, the direct progenitor of cultivated soybean, inhabits a wide distribution range across the mainland of east asia and the japanese archipelago. a multidisciplinary approach combining analyses of population genetics based on 20 nuclear microsatellites and one plastid locus were applied to reveal the genetic variation of wild soybean, and the contributions of geographical, environmental factors and historic climatic change on its patterns of genetic differentiation. high genetic diver ...201626952904
functional characterization of a glycine soja ca(2+)atpase in salt-alkaline stress responses.it is widely accepted that ca(2+)atpase family proteins play important roles in plant environmental stress responses. however, up to now, most researches are limited in the reference plants arabidopsis and rice. the function of ca(2+)atpases from non-reference plants was rarely reported, especially its regulatory role in carbonate alkaline stress responses. hence, in this study, we identified the p-type ii ca(2+)atpase family genes in soybean genome, determined their chromosomal location and gen ...201626801329
domestication footprints anchor genomic regions of agronomic importance in soybeans.present-day soybeans consist of elite cultivars and landraces (glycine max, fully domesticated (fd)), annual wild type (glycine soja, nondomesticated (nd)), and semi-wild type (semi-domesticated (sd)). fd soybean originated in china, although the details of its domestication history remain obscure. more than 500 diverse soybean accessions were sequenced using specific-locus amplified fragment sequencing (slaf-seq) to address fundamental questions regarding soybean domestication. in total, 64,141 ...201626479264
genetically modified and wild soybeans: an immunologic comparison.most traits introduced into genetically engineered crops result from the expression of new proteins. as the first step toward assessing the allergenic potential of genetically modified organism (gmo) food, immunologic and physicochemical characterizations are needed. we prepared crude extract from gmo soybeans, wild soybeans, curd, and soy milk and then performed sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). after acidification with hcl, the samples were separated to glob ...201616119037
a soybean quantitative trait locus that promotes flowering under long days is identified as ft5a, a flowering locus t ortholog.flowering locus t (ft) is an important floral integrator whose functions are conserved across plant species. in soybean, two orthologs, ft2a and ft5a, play a major role in initiating flowering. their expression in response to different photoperiods is controlled by allelic combinations at the maturity loci e1 to e4, generating variation in flowering time among cultivars. we determined the molecular basis of a quantitative trait locus (qtl) for flowering time in linkage group j (chromosome 16). f ...201627422993
proteomic and genetic analysis of glycinin subunits of sixteen soybean genotypes.we investigated proteomic and genomic profiles of glycinin, a family of major storage proteins in 16 different soybean genotypes consisting of four groups including wild soybean (glycine soja), unimproved cultivated soybean landraces from asia (g. max), ancestors of n. american soybean (g. max), and modern soybean (g. max) genotypes. we observed considerable variation in all five glycinin subunits, g1, g2 g3, g4 and g5 using proteomics and genetic analysis. two-dimensional polyacrylamide gel ele ...201617524657
nucleotide diversity patterns of three divergent soybean populations: evidences for population-dependent linkage disequilibrium and taxonomic status of glycine gracilis.the level of linkage disequilibrium (ld) is a major factor to determine dna polymorphism pattern of a population and to construct high-resolution maps useful in localizing and gene cloning of complicated traits. here, we investigated ld level of three soybean populations with different genetic backgrounds and taxonomic status of g. gracilis by comparing the dna polymorphism patterns of four high-diversity single-copy nuclear genes. a total of 152, 22, and 77 accessions of g. soja, g. gracilis, a ...201526442568
fingerprinting soybean germplasm and its utility in genomic research.the united states department of agriculture, soybean germplasm collection includes 18,480 domesticated soybean and 1168 wild soybean accessions introduced from 84 countries or developed in the united states. this collection was genotyped with the soysnp50k beadchip containing greater than 50k single-nucleotide polymorphisms. redundant accessions were identified in the collection, and distinct genetic backgrounds of soybean from different geographic origins were observed that could be a unique re ...201526224783
protamine sulfate precipitation method depletes abundant plant seed-storage proteins: a case study on legume plants.depletion of abundant proteins is one of the effective ways to improve detection and identification of low-abundance proteins. our previous study showed that protamine sulfate precipitation (psp) method can deplete abundant ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) from leaf proteins and is suitable for their in-depth proteome investigation. in this study, we provide evidence that the psp method can also be effectively used for depletion of abundant seed-storage proteins (ssps) f ...201525641780
heterosis and combining ability estimates in isoflavone content using different parental soybean accessions: wild soybean, a valuable germplasm for soybean breeding.isoflavone, a group of secondary metabolites in soybean, is beneficial to human health. improving isoflavone content in soybean seeds has become one of the most important breeding objectives. however, the narrow genetic base of soybean cultivars hampered crop improvement. wild soybean is an extraordinarily important gene pool for soybean breeding. in order to select an optimal germplasm for breeding programs to increase isoflavone concentration, 36 f1 soybean progenies from different parental ac ...201525607952
genetic diversity of wild soybean populations in dongying, china, by simple sequence repeat analysis.annual wild soybean (glycine soja sieb. et zucc.), the ancestor of cultivated soybean (g. max), is believed to be a potential gene source for further improvement of soybean to cope with environmental stress. in this study, 10 simple sequence repeat (ssr) markers were used to evaluate the genetic diversity and population genetic structure in five wild soybean populations using 195 accessions collected from dongying, china. ten ssr markers yielded 90 bands, with an average of nine bands per marker ...201526436402
[occurrence characteristics and molecular genetic basis of pod shattering in soybean].pod shattering is a natural property of wild soybean (glycine soja) for propagation and also a major cause of yield loss in cultivated soybean (glycine max l. merr). thus, studies on occurrence characteristics and molecular genetic basis of pod shattering in soybean can provide insights into both molecular mechanisms and potential application in legume crop improvement. in this review, we summarize the occurrence features and phenotypic identification methods of pod shattering based on analysis ...201526351049
comparative biochemical and proteomic analyses of soybean seed cultivars differing in protein and oil content.this study develops differential protein profiles of soybean (glycine max) seeds (cv. saedanbaek and daewon) varying in protein (47.9 and 39.2%) and oil (16.3 and 19.7%) content using protamine sulfate (ps) precipitation method coupled with a 2d gel electrophoresis (2dge) approach. of 71 detected differential spots between daewon and saedanbaek, 48 were successfully identified by maldi-tof/tof. gene ontology analysis revealed that up-regulated proteins in saedanbaek were largely associated with ...201526237057
gmhs1-1, encoding a calcineurin-like protein, controls hard-seededness in soybean.loss of seed-coat impermeability was essential in the domestication of many leguminous crops to promote the production of their highly nutritious seeds. here we show that seed-coat impermeability in wild soybean is controlled by a single gene, gmhs1-1, which encodes a calcineurin-like metallophosphoesterase transmembrane protein. gmhs1-1 is primarily expressed in the malpighian layer of the seed coat and is associated with calcium content. the transition from impermeability to permeability in do ...201526098868
functional marker development of mir1511-indel and allelic diversity within the genus glycine.single-stranded non-protein coding small rnas, 18-25 nucleotides in length, are ubiquitous throughout plants genomes and are involved in post-transcriptional gene regulation. several types of dna markers have been reported for the detection of genetic diversity or sequence variation in soybean, one of the most important legume crops in worldwide for seed protein and oil content. recently, with the available of public genomic databases, there has been a shift from the labor-intensive development ...201526084707
a single-nucleotide polymorphism in an endo-1,4-β-glucanase gene controls seed coat permeability in soybean.physical dormancy, a structural feature of the seed coat known as hard seededness, is an important characteristic for adaptation of plants against unstable and unpredictable environments. to dissect the molecular basis of qhs1, a quantitative trait locus for hard seededness in soybean (glycine max (l) merr.), we developed a near-isogenic line (nil) of a permeable (soft-seeded) cultivar, tachinagaha, containing a hard-seed allele from wild soybean (g. soja) introduced by successive backcrossings. ...201526039079
sequence and expression variations suggest an adaptive role for the da1-like gene family in the evolution of soybeans.the da1 gene family is plant-specific and arabidopsis da1 regulates seed and organ size, but the functions in soybeans are unknown. the cultivated soybean (glycine max) is believed to be domesticated from the annual wild soybeans (glycine soja). to evaluate whether da1-like genes were involved in the evolution of soybeans, we compared variation at both sequence and expression levels of da1-like genes from g. max (gmada1) and g. soja (gsoda1).201525975199
genetic variants in root architecture-related genes in a glycine soja accession, a potential resource to improve cultivated soybean.root system architecture is important for water acquisition and nutrient acquisition for all crops. in soybean breeding programs, wild soybean alleles have been used successfully to enhance yield and seed composition traits, but have never been investigated to improve root system architecture. therefore, in this study, high-density single-feature polymorphic markers and simple sequence repeats were used to map quantitative trait loci (qtls) governing root system architecture in an inter-specific ...201525765991
development, validation and genetic analysis of a large soybean snp genotyping array.cultivated soybean (glycine max) suffers from a narrow germplasm relative to other crop species, probably because of under-use of wild soybean (glycine soja) as a breeding resource. use of a single nucleotide polymorphism (snp) genotyping array is a promising method for dissecting cultivated and wild germplasms to identify important adaptive genes through high-density genetic mapping and genome-wide association studies. here we describe a large soybean snp array for use in diversity analyses, li ...201525641104
fine-scale phylogenetic structure and major events in the history of the current wild soybean (glycine soja) and taxonomic assignment of semi-wild type (glycine gracilis skvortz.) within the chinese subgenus soja.wild and cultivated species of soybeans have coexisted for 5000 years in china. despite this long history, there is very little information on the genetic relationship of glycine soja and g. max. to gain insight into the major events in the history of the subgenus soja, we examined 20 simple sequence repeat (ssr) markers of a large number of accessions (910). the results showed no significant differences between wild and semi-wild soybeans in genetic diversity but significant differences between ...201521984661
genetic diversity and the conservation priority of glycine soja populations from northern china.knowledge of the spatial patterns of genetic variation in wild populations has significant implications for in situ conservation and the determination of conservation order. to study the levels of genetic diversity, spatial genetic structures, and genetic distances in glycine soja, 11 natural populations in northern china were analyzed by estimating genetic coefficients using inter-simple sequence repeat (issr) fingerprints via mixed sampling strategies. sixteen issr primers generated 98 reprodu ...201526681007
gscml27, a gene encoding a calcium-binding ef-hand protein from glycine soja, plays differential roles in plant responses to bicarbonate, salt and osmotic stresses.calcium, as the most widely accepted messenger, plays an important role in plant stress responses through calcium-dependent signaling pathways. the calmodulin-like family genes (cmls) encode ca2+ sensors and function in signaling transduction in response to environmental stimuli. however, until now, the function of plant cml proteins, especially soybean cmls, is largely unknown. here, we isolated a glycine soja cml protein gscml27, with four conserved ef-hands domains, and identified it as a cal ...201526550992
[distribution characteristics of soil nematodes in reclaimed land of copper-mine-tailings in different plant associations].a survey was carried out to investigate soil nematode communities in the plant associations of gramineae (arthraxon lanceolatus, al; imperata cylindrica, ic) and leguminosae (glycine soja, gs) in reclaimed land of copper-mine-tailings and in the plant associations of gramineae (digitaria chrysoblephara, dc-ck) of peripheral control in fenghuang mountain, tongling city. a total of 1277 nematodes were extracted and sorted into 51 genera. the average individual density of the nematodes was 590 indi ...201526094476
ectopic expression of a glycine soja myo-inositol oxygenase gene (gsmiox1a) in arabidopsis enhances tolerance to alkaline stress.myo-inositol participates in various aspects of plant physiology, and myo-inositol oxygenase is the key enzyme of the myo-inositol oxygenation pathway. previous studies indicated that myo-inositol oxygenase may play a role in plant responses to abiotic stresses. in this study, we focused on the functional characterization of gsmiox1a, a remarkable alkaline stress-responsive gene of glycine soja 07256, based on rna-seq data. using quantitative real-time pcr, we demonstrated that gsmiox1a is rapid ...201526091094
overexpression of gsgstu13 and scmrp in medicago sativa confers increased salt-alkaline tolerance and methionine content.tau-class glutathione s-transferases (gstus) are ubiquitous proteins encoded by a large gene family in plants, which play important roles in combating different environmental stresses. in previous studies, we constructed a glycine soja transcriptional profile, and identified three gstus (gsgstu13, gsgstu14 and gsgstu19) as potential salt-alkaline stress-responsive genes. two of them, gsgstu14 and gsgstu19, have been shown to positively regulate plant salt-alkaline tolerance. in this study, we fu ...201526010993
wild soybean roots depend on specific transcription factors and oxidation reduction related genesin response to alkaline stress.soil alkalinity is an important environmental problem limiting agricultural productivity. wild soybean (glycine soja) shows strong alkaline stress tolerance, so it is an ideal plant candidate for studying the molecular mechanisms of alkaline tolerance and identifying alkaline stress-responsive genes. however, limited information is available about g. soja responses to alkaline stress on a genomic scale. therefore, in the present study, we used rna sequencing to compare transcript profiles of g. ...201525874911
gsskp21, a glycine soja s-phase kinase-associated protein, mediates the regulation of plant alkaline tolerance and aba sensitivity.plant skp1-like family proteins, components of the scf complex e3 ligases, are involved in the regulation of plant development and stress responses. little is known about the precise function of skp genes in plant responses to environmental stresses. gsskp21 was initially identified as a potential stress-responsive gene based on the transcriptome sequencing of glycine soja. in this study, we found that gsskp21 protein contains highly conserved skp domains in its n terminus and an extra unidentif ...201525477077
a putative soybean gmssos1 confers enhanced salt tolerance to transgenic arabidopsis sos1-1 mutant.the cdna of gmssos1, a putative plasma membrane na(+)/h(+) antiporter gene isolated from glycine max, glycine soja, and their hybrid, was constructed into plant expression vector pcambia 1300 and then transformed with agrobacterium tumefaciens under the control of camv 35s promoter to arabidopsis thaliana wild-type (wt) and mutant (atsos1-1) plants. by hygromycin resistance detection and pcr analysis, transgenic plants (wt35s:gmssos1 and atsos1-1 35s:gmssos1) were obtained. seed germination, see ...201524934653
a 14-3-3 family protein from wild soybean (glycine soja) regulates aba sensitivity in arabidopsis.it is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. by conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in aba responses. hence, in this study, based on the latest glycine max genome on phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, ...201526717241
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