Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
phenol degradation activity and reusability of corynebacterium glutamicum coated with nh(2)-functionalized silica-encapsulated fe(3)o(4) nanoparticles.in this study, a novel method to immobilize and separate corynebacterium glutamicum for phenol degradation was developed using fe(3)o(4) nanoparticles (nps). the fe(3)o(4) nps were encapsulated with silica and functionalized with nh(2) groups to enhance their capacity to adsorb on the cell surface. the results showed that the nh(2)-functionalized silica-encapsulated fe(3)o(4) nps strongly adsorbed on the cell surface of c. glutamicum during 32d culture without any interruptions of their normal c ...201222093979
Bacteremia following dental implant surgery: Preliminary results.Objectives: The aims of this study were to investigate the incidence of bacteremia, bacteriology and antibiotic susceptibility against to causative bacteria associated with dental implant installation. Study Design: 30 generally healthy patients were enrolled in this study. Blood samples were collected at baseline and at 30 minutes after dental implant installation and 24 hours after dental implant surgery. Blood samples were cultured in a BACTEC system. The isolated bacteria were identified us ...201222157668
Characterization and Comparison of Invasive Corynebacterium diphtheriae Isolates from France and Poland.Corynebacterium diphtheriae, the agent of diphtheria, is rarely responsible for bacteremia. However, high numbers of bacteremia have been reported in countries with extensive immunization coverage. Here, we used molecular and phenotypic tools to characterize and compare 42 invasive isolates collected in France (including New Caledonia) and Poland over a 23-year period.201222090411
Efficient aerobic succinate production from glucose in minimal medium with Corynebacterium glutamicum.Corynebacterium glutamicum, an established industrial amino acid producer, has been genetically modified for efficient succinate production from the renewable carbon source glucose under fully aerobic conditions in minimal medium. The initial deletion of the succinate dehydrogenase genes (sdhCAB) led to an accumulation of 4.7 g l(-1) (40 mM) succinate as well as high amounts of acetate (125 mM) as by-product. By deleting genes for all known acetate-producing pathways (pta-ackA, pqo and cat) ace ...201222018023
Construction of a novel sacB-based system for marker-free gene deletion in Corynebacterium glutamicum.Bacillus subtilis sacB gene with its 463bp upstream region including its native promoter has been used for marker-free gene deletion in Corynebacterium glutamicum, but the role of this upstream region is not clear. In this study, it was demonstrated that the upstream region of sacB failed to efficiently promote its expression in C. glutamicum, and the native promoter of sacB is weak in C. glutamicum. The expression level of sacB under its native promoter in C. glutamicum is not high enough for c ...201222100974
identification of non-diphtheriae corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.we evaluated the bruker biotyper matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry for identification of 92 clinical isolates of corynebacterium species in comparison to identification using rpob or 16s rrna gene sequencing. eighty isolates (87%) yielded a score of ≥1.700, and all of these were correctly identified to the species level with the exception of corynebacterium aurimucosum being misidentified as the closely related corynebacterium minutissimum.201222075579
genomic library screening for viruses from the human dental plaque revealed pathogen-specific lytic phage sequences.bacterial pathogenesis presents an astounding arsenal of virulence factors that allow them to conquer many different niches throughout the course of infection. principally fascinating is the fact that some bacterial species are able to induce different diseases by expression of different combinations of virulence factors. nevertheless, studies aiming at screening for the presence of bacteriophages in humans have been limited. such screening procedures would eventually lead to identification of p ...201221969025
Physiology and global gene expression of a Corynebacterium glutamicum ?F(1)F(O)-ATP synthase mutant devoid of oxidative phosphorylation.A mutant of Corynebacterium glutamicum ATCC 13032 with a deletion of the atpBEFHAGDC genes encoding F(1)F(O)-ATP synthase was characterized. Whereas no growth was observed with acetate as sole carbon source, the ?F(1)F(O) mutant reached 47% of the growth rate and 65% of the biomass of the wild type during shake-flask cultivation in glucose minimal medium. Initially, the mutant strain showed a strongly increased glucose uptake rate accompanied by a high oxygen consumption rate and pyruvate secret ...201222050934
genome sequence of corynebacterium glutamicum atcc 14067, which provides insight into amino acid biosynthesis in coryneform bacteria.we report the genome sequence of corynebacterium glutamicum atcc 14067 (once named brevibacterium flavum), which is useful for taxonomy research and further molecular breeding in amino acid production. preliminary comparison with those of the reported coryneform strains revealed some notable differences that might be related to the difficulties in molecular manipulation.201222247536
genome sequence of corynebacterium casei ucma 3821, isolated from a smear-ripened cheese.corynebacterium casei is one of the most prevalent species present on the surfaces of smear-ripened cheeses, where it contributes to the production of the desired organoleptic properties. here, we report the draft genome sequence of corynebacterium casei ucma 3821 to provide insights into its physiology.201222247534
efflux permease cgacr3-1 of corynebacterium glutamicum is an arsenite-specific antiporter.resistance to arsenite (as(iii)) by cells is generally accomplished by arsenite efflux permeases from acr3 or arsb unrelated families. we analyzed the function of three acr3 proteins from corynebacterium glutamicum, cgacr3-1, cgacr3-2, and cgacr3-3. cgacr3-1 conferred the highest level of as(iii) resistance and accumulation in vivo. cgacr3-1 was also the most active when everted membranes vesicles from escherichia coli or c. glutamicum mutants were assayed for efflux with different energy source ...201222102279
engineering corynebacterium glutamicum for the production of pyruvate.a corynebacterium glutamicum strain with inactivated pyruvate dehydrogenase complex and a deletion of the gene encoding the pyruvate:quinone oxidoreductase produces about 19 mm l: -valine, 28 mm l: -alanine and about 55 mm pyruvate from 150 mm glucose. based on this double mutant c. glutamicum △acee △pqo, we engineered c. glutamicum for efficient production of pyruvate from glucose by additional deletion of the ldha gene encoding nad(+)-dependent l: -lactate dehydrogenase (ldha) and introduction ...201222228312
regulation of the malic enzyme gene male by the transcriptional regulator malr in corynebacterium glutamicum.corynebacterium glutamicum is a gram-positive nonpathogenic bacterium that is used for the biotechnological production of amino acids. here, we investigated the transcriptional control of the male gene encoding malic enzyme (male) in c. glutamicum atcc13032, which is known to involve the nitrogen regulator amtr. gel shift experiments using purified regulators rama and ramb revealed binding of these regulators to the male promoter. in dna-affinity purification experiments a hitherto uncharacteriz ...201222261175
corynebacterium diphtheriae 67-72p hemagglutinin, characterized as the protein dip0733, contributes to invasion and induction of apoptosis in hep-2 cells.although corynebacterium diphtheriae has been classically described as an exclusively extracellular pathogen, there is growing evidence that it may be internalized by epithelial cells. the aim of the present report was to investigate the nature and involvement of the surface-exposed non-fimbrial 67-72 kda proteins (67-72p), previously characterized as adhesin/hemagglutinin, in c. diphtheriae internalization by hep-2 cells. transmission electron microscopy and bacterial internalization inhibition ...201222239957
culture-independent exploration of the teat apex microbiota of dairy cows reveals a wide bacterial species diversity.due to their close proximity to the mammary gland tissue, the bacterial communities lining the teat apex of the udders from lactating cows influence udder health. denaturing gradient gel electrophoresis of the amplified v3 variable region of the 16s rrna gene was used as a culture-independent method to reveal the bacterial composition of 48 samples originating from the teat apices of twelve friesian-holstein dairy cows suffering from clinical mastitis in one quarter. the microbiota belonged to f ...201122266158
[effect of gamma-glutamyl kinase gene knock-out on metabolism in l-arginine-producing strain corynebacterium crenatum 8-193].in order to optimize precursor supply for l-arginine biosynthesis, we constructed a corynebacterium crenatum 8-193 mutant with gamma-glutamyl kinase gene (prob) in-frame deletion. the effects of prob knock-out on physiological characteristics of the mutant were investigated.201122260045
Prevalence of Corynebacterium ulcerans in dogs residing in Osaka, Japan.Diphtheria-like human illness caused by Corynebacterium ulcerans is an emerging threat in developed countries, with incidence sometimes higher than that of diphtheria caused by Corynebacterium diphtheriae. Companion animals are considered a potential source of human infections. In order to determine the prevalence of C. ulcerans among dogs, we performed a screening for the bacterium in 583 dogs in the custody of the Osaka Prefectural government. Forty-four dogs (7.5%) were positive for the bacte ...201121921111
isolation and characterization of lipase-producing bacteria in the intestine of the silkworm, bombyx mori, reared on different forage.abstract the silkworm, bombyx mori l. (lepidoptera: bombycidae), an oligophagous insect that mainly feeds on mulberry leaves, is susceptible to entomopathogen infection when reared with tricuspid cudrania leaves. a total of 56 dominant bacterial strains, classified into 12 phylotypes based on bacteriological properties and analysis of 16s rrna genes, were isolated from the intestine of the fourth and fifth instar silkworm larvae. ten and seven phylotypes exist in the intestine of the silkworm ...201122243438
Site-Directed Mutagenesis Studies on the L: -Arginine-Binding Sites of Feedback Inhibition in N-Acetyl-L: -glutamate Kinase (NAGK) from Corynebacterium glutamicum.Arginine biosynthesis in Corynebacterium glutamicum proceeds via a pathway that is controlled by arginine through feedback inhibition of NAGK, the enzyme that converts N-acetyl-L: -glutamate (NAG) to N-acety-L: -glutamy-L: -phosphate. In this study, the gene argB encoding NAGK from C. glutamicum ATCC 13032 was site-directed, and the L: -arginine-binding sites of feedback inhibition in Cglu_NAGK are described. The N-helix and C-terminal residues were first deleted, and the results indicated that ...201122101454
Biotin protein ligase from Corynebacterium glutamicum: role for growth and L: -lysine production.Corynebacterium glutamicum is a biotin auxotrophic Gram-positive bacterium that is used for large-scale production of amino acids, especially of L: -glutamate and L: -lysine. It is known that biotin limitation triggers L: -glutamate production and that L: -lysine production can be increased by enhancing the activity of pyruvate carboxylase, one of two biotin-dependent proteins of C. glutamicum. The gene cg0814 (accession number YP_225000) has been annotated to code for putative biotin protein li ...201122159614
antimicrobial drug resistance in corynebacterium diphtheriae mitis. 201122099107
site-directed mutagenesis and feedback-resistant n-acetyl-l: -glutamate kinase (nagk) increase corynebacterium crenatum l: -arginine production.n-acetyl-l: -glutamate kinase (ec 2.7.2.8) is first committed in the specific l: -arginine pathway of corynebacterium sp. a limited increase of l: -arginine production for the argb overexpression in the engineering c. creantum sypa-ccb strain indicated that l: -arginine feedback inhibition plays an influence on the l: -arginine production. in this study, we have performed site-directed mutagenesis of the key enzyme (nagk) and the three mutations (e19r, h26e and h268d) exhibited the increase of i ...201121901472
Diphtheria-like Illness due to Corynebacterium Ulcerans infection. 201122028605
biochemical disclosure of the mycolate outer membrane of corynebacterium glutamicum.corynebacterineae is a specific sub-order of gram-positive bacteria that includes mycobacterium tuberculosis and corynebacterium glutamicum. their cell wall is composed of a heteropolymer of peptidoglycan (pg) linked to arabinogalactan (ag), which in turn, is covalently associated to an atypical outer membrane hereafter called mycomembrane (m). this latter structure has been visualized by cryoelectron microscopy of vitreous sections but its biochemical composition is still poorly defined, thereb ...201122123248
The prevalence of interdigital erythrasma in southern region of Turkey.Background Erythrasma is a skin infection which is caused by Corynebacterium minutissimum. Interdigital erythrasma is the most common form. Objective The aim of this study was to detect the frequency and risk factors of interdigital erythrasma in patients with clinically suspected tinea pedis. Methods This study was conducted between June and December 2010 and included 122 patients who had interdigital foot lesions. All patients were examined using a Wood's lamp. The smears were stained using ...201121977942
[The use of MLVA for Corynebacterium diphtheriae genotyping--preliminary studies].The complete genome sequence of strain NCTC 13129 C. diphtheriae were investigated in order to identify tandem repeats (VNTR). From 75 VNTR loci identified in the genome 14 were selected. Primers were designed and PCR conditions were optimized for amplification of the selected VNTR markers. Preliminary studies of usefulness of selected VNTR markers were conducted using a group of 28 C. diphtheriae strains. From 14 markers 8 were regarded as potentially useful. The diversity of individual markers ...201122184915
Backbone and side chain (1)H, (13)C and (15)N resonance assignments of the catalytic domain of diphtheria toxin.Diphtheria is a serious upper respiratory tract disease caused by the diphtheria toxin (DT) secreted from the bacteria Corynebacterium diphtheriae. Vaccination is the best way to protect against this infectious disease. Diphtheria vaccines are prepared by isolating, purifying and chemically deactivating DT. Although toxoids have been used for decades in immunization, there is still little understanding at the molecular level of the process of toxoid preparation, and how chemical treatment enhanc ...201122205447
[bacteriology of ethmoidal sinus in chronic rhinosinusitis with and without nasal polyps].to compare the bacteriologic features of anterior ethmoidal biopsy specimens between chronic rhinosinusitis without nasal polyps (crsnp-), chronic rhinosinusitis with nasal polyps(crsnp+) and control patients.201121845823
bio-based production of chemicals, materials and fuels -corynebacterium glutamicum as versatile cell factory.since their discovery almost 60 years ago, corynebacterium glutamicum and related subspecies are writing a remarkable success story in industrial biotechnology. today, these gram-positive soil bacteria, traditionally well-known as excellent producers of l-amino acids are becoming flexible, efficient production platforms for various chemicals, materials and fuels. this development is intensively driven by systems metabolic engineering concepts integrating systems biology and synthetic biology int ...201122138494
a disarticulating spinal lesion. bacterial abscess. 201121905296
high sero-prevalence of caseous lymphadenitis identified in slaughterhouse samples as a consequence of deficiencies in sheep farm management in the state of minas gerais, brazil.abstract:201122067701
Corynebacterium epidermidicanis sp. nov., isolated from a dog's skin.A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the skin of a dog, designated strain 410T, was subjected to comprehensive taxonomic characterization. Comparison of the 16S rRNA gene sequence showed highest similarities to Corynebacterium diphtheriae, Corynebacterium pseudotuberculosis, and Corynebacterium ulcerans (96.1-96.7%). The quinone system consisted predominantly of MK-8(H2) and MK-9(H2). The polar lipid profile of strain 410T contained the major compounds d ...201122081710
Corynebacterium striatum bacteremia associated with central venous catheter infection.Corynebacterium striatum (C striatum) has been considered a contaminant of blood culture in past decades. Here we report the case of a patient with acute deterioration of chronic renal failure. She received hemodialysis and died from C striatum bacteremia. By using a randomly amplified polymorphic DNA (RAPD) method, we found that an association existed between C striatum from the bloodstream and that from the central venous catheter. We suggest that C striatum could be a pathogen of bloodstream ...201122154992
photoletter to the editor: trichomycosis (trichobacteriosis) axillaris.trichomycosis axillaris is a common tropical disease usually affecting the hair shafts of the axillae, characterized by nodular concretions along the hair shafts caused by corynebacterium tenuis. we describe a 38-year-old patient with trichomycosis axillaris. treatment, which included shaving of affected hair, followed by topical 3% erythromycin cream and clotrimazole powder was fully effective.201121894255
Canada's first case of a multidrug-resistant Corynebacterium diphtheriae strain, isolated from a skin abscess.A toxigenic Corynebacterium diphtheriae biovar mitis sequence type 136 (ST136) strain was recovered from a toe infection of an unvaccinated patient recently returned from India. The isolate was resistant to clindamycin, erythromycin (ermX positive), tetracycline, and trimethoprim-sulfamethoxazole, intermediate to ceftriaxone and cefotaxime, and had high MICs for telithromycin and chloramphenicol but was sensitive to other drugs.201121880960
[A frequently overlooked bacteria in clinical microbiology laboratories: arcanobacterium haemolyticum].Arcanobacterium haemolyticum, previously known as Corynebacterium haemolyticum, is a facultative anaerobic, gram-positive bacillus with negative catalase and positive CAMP inhibition test results. It may be the causative agent of about 0.5-3% of acute bacterial pharyngitis especially in children and young adults. Since growth of A.haemolyticum is usually inhibited by flora members and since it slowly develops hemolysis in sheep blood agar and its colony morphology resembles beta-hemolytic strept ...201121935787
conformational changes of the betaine transporter betp from corynebacterium glutamicum studied by pulse epr spectroscopy.the betaine transporter betp from corynebacterium glutamicum is activated by hyperosmotic stress critically depending on the presence and integrity of its sensory c-terminal domain. the conformational properties of the trimeric betp reconstituted in liposomes in the inactive state and during osmotic activation were investigated by site-directed spin labeling and electron paramagnetic resonance (epr) spectroscopy. comparison of intra- and intermolecular inter spin distance distributions obtained ...201122051018
[determination of causative agents in ocular infection of external adnexa and anterior segments--multicenter study of causative agents and drug sensitivity of ocular infection by the japanese association for ocular infection part i].to determine the causative agents of ocular infection of external adnexa and anterior segments due to selected criteria.201122073597
Inflammation regulates microRNA expression in cooperation with p53 and nitric oxide.microRNA (miRNA) are small non-coding RNA targeting mRNAs leading to their instability and diminished translation. Altered expression of miRNA is associated with cancer. Inflammation and nitric oxide modulates the development of lymphomas in p53 knockout mice and there exists a negative feedback loop between p53 and NOS2. Using a genetic strategy, we tested the hypothesis that inflammation-induced oxidative and nitrosative stress modulates miRNA expression in mouse model deficient in either p53 ...201122042537
Etiology and epidemiological analysis of glaucoma-filtering bleb infections in a tertiary eye care hospital in South India.To evaluate the microbial etiology and associated risk factors among patients with blebitis following trabeculectomy.201122011488
Studies on antimicrobial activities of solvent extracts of different spices.The antimicrobial activities of the ethyl acetate, acetone and methanol extract of 12 plant species were studied. The extract of Capsicum annuum (red pepper) (fruit) Zingiber officinale (ginger) (root), Cuminum cyminum (cumin), Alpinia ficinarum (galingale), Coriandrum sativum (coriander), Cinnamomun zeylanicum Nees (cinnamomun), Origanum onites L. (thyme), Folium sennae (senna), Eugenia caryophyllata (cloves), Flos tiliae (lime), Folium menthae crispae (peppermint) and Piper nigrum (blackpepper ...201121882663
Infectious endocarditis caused by Nocardia sp.: histological morphology as a guide for the specific diagnosis.Nocardia is a rare opportunistic agent, which may affect immunocompromised individuals causing lung infections and exceptionally infective endocarditis (IE). There are few reports of IE caused by Nocardia sp., usually involving biological prostheses but rarely in natural valves. Its accurate microbiological identification may be hampered by the similarity with Rhodococcus equi and Corynebacterium spp. Here we report a case of native mitral valve IE caused by this agent in which the clinical abse ...201121861012
molecular characterization of prpr, the transcriptional activator of propionate catabolism in corynebacterium glutamicum.the 2-methylcitrate cycle is used to metabolize propionate in corynebacterium glutamicum. the regulator, prpr (cg0800), of the prpdbc2 operon was identified and characterized. the regulator has no similarities to the up to now known prpr regulators from other organisms. growth of a δprpr mutant revealed severe growth deficits and a prolonged lag phase if propionate was present in the medium. transcriptome analyses demonstrated the inability of the δprpr strain to induce the prpdbc2 genes in the ...201121933687
Urosepsis caused by Globicatella sanguinis and Corynebacterium riegelii in an adult: case report and literature review.We report an extremely rare case of urosepsis caused by Globicatella sanguinis and Corynebacterium riegelii coinfection in a 94-year-old Japanese man with nephrolithiasis. Prompt identification of this coinfection is important so that effective antimicrobial coverage can be initiated.201122080192
Heterologous and homologous expression of the arginine biosynthetic argC~H cluster from Corynebacterium crenatum for improvement of L: -arginine production.The genes involved in L: -arginine biosynthesis in Corynebacterium crenatum are organized as the argCJBDFRGH cluster like in Corynebacterium glutamicum. However, the argC~H cluster of the C. crenatum SYPA 5-5, which is an industrialized L: -arginine producer, had a lethal mutation occurring in the ArgR repressor encoding gene. The argC~H cluster with an inactive argR was overexpressed in E. coli and C. crenatum. In the recombinant E. coli JM109 enzyme activities were increased, and more L: -argi ...201122009057
activity of daptomycin and three comparator agents against non-diphtheriae corynebacterium isolates of clinical interest. 201122129591
Bacteriology of hidradenitis suppurativa exacerbations. Deep tissue cultures obtained during carbon dioxide laser treatment.Background: Previous studies have shown different bacteria in hidradenitis suppurativa (HS) lesions, but the literature regarding bacteria in acute exacerbation of HS is sparse. Objectives: The aim of the study was to determine the type of bacteria isolated from HS lesions during exacerbation of the disease. Methods: HS patients with acute nodules or abscesses were examined and treated by carbon dioxide laser vaporisation. Bacterial samples for aerobic and anaerobic cultures were taken from t ...201122098253
Impact of a new glucose utilization pathway in amino acid-producing Corynebacterium glutamicum.Corynebacterium glutamicum imports and phosphorylates glucose, fructose and sucrose by the phosphoenolpyruvate-dependent phosphotransferase carbohydrate uptake system (PTS). Recently, we have discovered how glucose can be utilized by C. glutamicum in a PTS-independent manner. PTS-independent glucose uptake is mediated by one of two inositol permeases (IolT1 or IolT2) and the second function of PTS, substrate phosphorylation, is catalyzed by one of two glucokinases (Glk or PpgK). PTS-deficient C. ...201122008639
Antibiotic susceptibility of Corynebacterium minutissimum isolated from lesions of Turkish patients with erythrasma. 201122082845
Characterization of Corynebacterium diphtheriae isolates from infected skin lesions in the Northern Territory of Australia.Corynebacterium diphtheriae is commonly isolated from cutaneous skin lesions in the Northern Territory of Australia. We prospectively assessed 32 recent isolates from infected skin lesions, in addition to reviewing 192 isolates collected over 5 years for toxin status. No isolates carried the toxin gene. Toxigenic C. diphtheriae is now a rare occurrence in the Northern Territory.201121918025
[Genotyping and molecular marking of bacteria and viruses in epidemiological surveillance of actual infections].Determination of genetic and molecular features of pathogens circulating in Russia, in the northwest of the country and in St. Petersburg to resolve the problems of spread of diseases caused by these pathogens.201121913388
The glgB-encoded glycogen branching enzyme is essential for glycogen accumulation in Corynebacterium glutamicum.Corynebacterium glutamicum transiently accumulates glycogen as carbon capacitor during the early exponential growth phase in media containing carbohydrates. In some bacteria glycogen is synthesized by the consecutive action of ADP-glucose pyrophosphorylase (GlgC), glycogen synthase (GlgA) and glycogen branching enzyme (GlgB). GlgC and GlgA of C. glutamicum have been shown to be necessary for glycogen accumulation in this organism. However, although cg1381 has been annotated as the putative C. gl ...201121903753
deletion of the aconitase gene in corynebacterium glutamicum causes strong selection pressure for secondary mutations inactivating citrate synthase.the aconitase gene acn of corynebacterium glutamicum is regulated by four transcriptional regulators, indicating that the synthesis of this enzyme is carefully controlled. to understand the causes for this elaborate regulation, the properties of the δacn-1 deletion mutant were analyzed in detail. the mutant was glutamate auxotrophic in glucose minimal medium, showed a strong growth defect, and secreted large amounts of acetate. none of these phenotypes could be complemented by plasmid-encoded ac ...201121984793
Corynebacterium pyruviciproducens promotes the production of ovalbumin specific antibody via stimulating dendritic cell differentiation and up-regulating Th2 biased immune response.Corynebacterium pyruviciproducens (C. pyruviciproducens), a newly discovered Corynebacterium, is gram-positive, non-flagellate, non-spore-forming lipophilic rod. No known pathogenic components of Corynebacteria have been found in this new bacterium, such as diphtheria toxin and tuberculostearic acid. In the present study, referring to Propionibacterium acnes (P. acnes), a well-known bacterial adjuvant, the stimulation of dendritic cells by C. pyruviciproducens was analyzed through detecting the ...201122178104
role of bundle helices in a regulatory crosstalk in the trimeric betaine transporter betp.the na(+)-coupled betaine symporter betp regulates transport activity in response to hyperosmotic stress only in its trimeric state, suggesting a regulatory crosstalk between individual protomers. betp shares the overall fold of two inverted structurally related five-transmembrane (tm) helix repeats with the sequence-unrelated na(+)-coupled symporters leut, vsglt, and mhp1, which are neither trimeric nor regulated in transport activity. conformational changes characteristic for this transporter ...201122024596
erratum to: corynebacterium glutamicum as a potent biocatalyst for the bioconversion of pentose sugars to value-added products. 201122159741
current knowledge on isobutanol production with escherichia coli, bacillus subtilis and corynebacterium glutamicum.due to steadily rising crude oil prices great efforts have been made to develop designer bugs for the fermentative production of higher alcohols, such as 2-methyl-1-butanol, 3-methyl-1-butanol and 2-methyl-1-propanol (isobutanol), which all possess quality characteristics comparable to traditional oil based fuels. the common metabolic engineering approach uses the last two steps of the ehrlich pathway, catalyzed by 2-ketoacid decarboxylase and an alcohol dehydrogenase converting the branched cha ...201122008938
genome sequence of corynebacterium glutamicum s9114, a strain for industrial production of glutamate.here we report the genome sequence of corynebacterium glutamicum s9114, an industrial producer widely used in production of glutamate in china. preliminary comparison with the sequences of the corynebacterium glutamicum strains atcc 13032 and r revealed some notable mutagenesis that might be related to the high yield of glutamate.201121994927
quantitative lipid composition of cell envelopes of corynebacterium glutamicum elucidated through reverse micelle extraction.cells of the corynebacterium-nocardia-mycobacterium group of bacteria are surrounded by an outer membrane (om) containing mycolic acids that are covalently linked to the underlying arabinogalactan-peptidoglycan complex. this om presumably acts as a permeability barrier that imparts high levels of intrinsic drug resistance to some members of this group, such as mycobacterium tuberculosis, and its component lipids have been studied intensively in a qualitative manner over the years. however, the q ...201121876124
corynebacterium glutamicum as a potent biocatalyst for the bioconversion of pentose sugars to value-added products.corynebacterium glutamicum, the industrial microbe traditionally used for the production of amino acids, proved its value for the fermentative production of diverse products through genetic/metabolic engineering. a successful demonstration of the heterologous expression of arabinose and xylose utilization genes made them interesting biocatalysts for pentose fermentation, which are the main components in lignocellulosic hydrolysates. its ability to withstand substantial amount of general growth i ...201122094976
Corynebacterium glutamicum whcB, a stationary phase-specific regulatory gene.The function of whcB, one of the four whiB homologues of Corynebacterium glutamicum, was assessed. Cells carrying the P(180) -whcB clone, and thus overexpressing the whcB gene, showed retarded growth, probably due to increased sensitivity to oxidants, whereas cells lacking whcB (?whcB) did not. However, growth retardation was not observed in cells with additionally whcE deleted. Furthermore, the ?whcE phenotype, characterized by slow growth and sensitivity to oxidants, was reversed in cells carr ...201122098456
production of minicellulosomes for the enhanced hydrolysis of cellulosic substrates by recombinant corynebacterium glutamicum.although cellulosic materials of plant origin are the most abundant utilizable biomass resource, the amino acid-producing organism corynebacterium glutamicum can not utilize these materials. here we report the engineering of a c. glutamicum strain expressing functional minicellulosomes containing chimeric endoglucanase e bound to minicbpa from clostridium cellulovorans that can hydrolyze cellulosic materials. the chimeric endoglucanase e consists of the endoglucanase e catalytic backbone of clos ...201122112952
[corynebacterium pseudodiphtheriticum causing severe pneumonia in secondary immunoglobulin deficiency].corynebacterium pseudodiphtheriticum is of increasing importance because of the rising number of immunocompromised patients. pneumonia, but also endocarditis, urinary tract infections or keratitis can be caused by this bacteria in case of immunosuppression. taking corynebacterium pseudodiphtheriticum into consideration as causitive agent provides for a fast onset of targeted antibiotic therapy.201122109586
growth independent rhamnolipid production from glucose using the non-pathogenic pseudomonas putida kt2440.abstract:201121999513
indigenous soil bacteria with the combined potential for hydrocarbon consumption and heavy metal resistance.introduction: transconjugant bacteria with combined potential for hydrocarbon utilization and heavy metal resistance were suggested by earlier investigators for bioremediation of soils co-contaminated with hydrocarbons and heavy metals. the purpose of this study was to offer evidence that such microorganisms are already part of the indigenous soil microflora. methods: microorganisms in pristine and oily soils wer ...201121948132
[pitted keratolysis--how to treat?].pitted keratolysis is a common and problematic skin condition affecting the feet caused by the keratolytic activity of corynebacterium, micrococcus, dermatophilus. skin lesions are characterized by crater-like pits of the epidermis accompanied by a burning sensation, itching, pain and malodor. the purpose of the present paper was to characterise the disease's clinical features and possibilities for the therapy. due to its interdisciplinary nature, not only dermatologists ought to be familiar wit ...201121936353
Solution NMR structures reveal a distinct architecture and provide first structures for protein domain family PF04536.The protein family (Pfam) PF04536 is a broadly conserved domain family of unknown function (DUF477), with more than 1,350 members in prokaryotic and eukaryotic proteins. High-quality NMR structures of the N-terminal domain comprising residues 41-180 of the 684-residue protein CG2496 from Corynebacterium glutamicum and the N-terminal domain comprising residues 35-182 of the 435-residue protein PG0361 from Porphyromonas gingivalis both exhibit an a/ß fold comprised of a four-stranded ß-sheet, thre ...201122198206
molecular characterization of corynebacterium pseudotuberculosis isolates using eric-pcr.caseous lymphadenitis is an infectious sheep and goats disease caused by corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. c. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. the aim of this study is evaluating the potential of the enterobacterial repetitive intergenic consensus (eric-pcr) as a tool for molecular typing of c. pseudotuberculosis strains isolated in sh ...201121733644
Improving protein secretion of a transglutaminase-secreting Corynebacterium glutamicum recombinant strain on the basis of (13)C metabolic flux analysis.Corynebacterium glutamicum is known as a host species for amino acid production. This microorganism was recently noticed as a host that produces secreted proteins. In this study, we performed (13)C metabolic flux analysis ((13)C-MFA) on a recombinant C. glutamicum strain that secretes a heterologous transglutaminase (TGase) to improve TGase secretion. For the (13)C-MFA of a TGase-secreting C. glutamicum strain in batch cultivation, a (13)C-labeling experiment and measurement of mass isotopomer d ...201121903468
corynebacterium glutamicum rnase e/g-type endoribonuclease encoded by ncgl2281 is involved in the 5' maturation of 5s rrna.corynebacterium glutamicum has one rnase e/g ortholog and one rnase j ortholog but no rnase y. we previously reported that the c. glutamicum ncgl2281 gene encoding the rnase e/g ortholog complemented the rng::cat mutation in escherichia coli but not the rne-1 mutation. in this study, we constructed an ncgl2281 knockout mutant and found that the mutant cells accumulated 5s rrna precursor molecules. the processing of 16s and 23s rrna, trna, and tmrna was normal. primer extension analysis revealed ...201121717142
strain-dependent arthritogenic potential of the zoonotic pathogen corynebacterium ulcerans.during the last decade the majority of diphtheria cases in europe had corynebacterium ulcerans as the etiologic agent with dogs and cats as the reservoir hosts. however, little has been documented about the virulence factors of this zoonotic pathogen. to set up an in vivo experimental c. ulcerans infection model, conventional swiss webster mice were intravenously infected with different doses (from 1+ù10(7) to 5+ù10(9) bacteria per mouse) of c. ulcerans strains, namely 809 (from human lower resp ...201121742447
Corynebacterium glutamicum survives arsenic stress with arsenate reductases coupled to two distinct redox mechanisms.Arsenate reductases (ArsCs) evolved independently as a defence mechanism against toxic arsenate. In the genome of Corynebacterium glutamicum, there are two arsenic resistance operons (ars1 and ars2) and four potential genes coding for arsenate reductases (Cg_ArsC1, Cg_ArsC2, Cg_ArsC1' and Cg_ArsC4). Using knockout mutants, in vitro reconstitution of redox pathways, arsenic measurements and enzyme kinetics, we show that a single organism has two different classes of arsenate reductases. Cg_ArsC1 ...201122032722
metabolic engineering of cellular transport for overproduction of the platform chemical 1,5-diaminopentane in corynebacterium glutamicum.the present work describes the development of a superior strain of corynebacterium glutamicum for diaminopentane (cadaverine) production via metabolic engineering of cellular transport processes. in c. glutamicum dap-3c, a tailor-made producer, the diaminopentane forming enzyme, lysine decarboxylase, was inhibited in vivo by its end-product, suggesting a potential bottleneck at the level of the export. the previously proposed lysine exporter lyse was shown not to be involved in diaminopentane ex ...201121821142
bacterial classification using genomic fingerprints obtained by virtual hybridization.in silico genomic fingerprints were produced by virtual hybridization of 191 fully sequenced bacterial genomes using a set of 15,264 13-mer probes specially designed to produce universal whole genome fingerprints. a novel approach for constructing phylogenetic trees, based on comparative analysis of genomic fingerprints, was developed. the resultant bacterial phylogenetic tree had strong similarities to those produced from the alignment of conserved sequences. notably, the trees derived from the ...201121906634
improvement of the redox balance increases l-valine production by corynebacterium glutamicum under oxygen deprivation conditions.production of l-valine under oxygen deprivation conditions by corynebacterium glutamicum lacking the lactate dehydrogenase gene ldha and overexpressing the l-valine biosynthesis genes ilvbncde was repressed. this was attributed to imbalanced cofactor production and consumption in the overall l-valine synthesis pathway: two moles of nadh was generated and two moles of nadph was consumed per mole of l-valine produced from one mole of glucose. in order to solve this cofactor imbalance, the coenzyme ...201122138982
concurrent diphtheria and infectious mononucleosis: difficulties for management, investigation and control of diphtheria in developing countries.we report a case of concurrent diphtheria and infectious mononucleosis in a brazilian 11-year-old child. two days after started the specific treatment for diphtheria the case was discharged following clinical recovery. this case highlights the difficulties to diagnose diphtheria clinically in partially immunized individuals and for management and control of diphtheria in developing countries.201121680765
[The characteristics of Gram-positive bacteria isolated from skin lesions observed in ambulatory patients and the assessment of their susceptibility to antimicrobial drugs].The bacterial skin and soft-tissue infections occur commonly and are characterized by more or less intensified changes within skin and subcutaneous tissue. The bacterial skin infections give rise to significant therapeutic problems associated with increasing resistance etiological agents of these infections to antibiotics and chemotherapeutics. The aim of this study was to assess the distribution of various Gram-positive microorganisms in skin lesion observed in ambulatory patients in a period f ...201121853673
Enzymatic synthesis of S-adenosylhomocysteine: immobilization of recombinant S-adenosylhomocysteine hydrolase from Corynebacterium glutamicum (ATCC 13032).Recombinant S-adenosylhomocysteine hydrolase from Corynebacterium glutamicum (CgSAHase) was covalently bound to Eupergit® C. The maximum yield of bound protein was 91% and the catalytic efficiency was 96.9%. When the kinetic results for the immobilized enzyme were compared with those for the soluble enzyme, no decrease in the catalytic efficiency of the former was detected. Both soluble and immobilized enzymes showed similar optimum pH and temperature ranges. The reuse of immobilized CgSAHase ca ...201122202964
genetic and biochemical characterization of corynebacterium glutamicum atp phosphoribosyltransferase and its three mutants resistant to feedback inhibition by histidine.atp phosphoribosyltransferase (atp-prt) catalyzes the condensation of atp and prpp at the first step of histidine biosynthesis and is regulated by a feedback inhibition from product histidine. here, we report the genetic and biochemical characterization of such an enzyme, hisg(cg), from corynebacterium glutamicum, including site-directed mutagenesis of the histidine-binding site for the first time. gene disruption and complementation experiments showed that hisg(cg) is essential for histidine bi ...201122172596
locating an extracellular k+-dependent interaction site that modulates betaine-binding of the na+-coupled betaine symporter betp.betp, a trimeric na(+)-coupled betaine symporter, senses hyperosmotic stress via its cytoplasmic c-terminal domain and regulates transport activity in dependence of the cytoplasmic k(+)-concentration. this transport regulation of betp depends on a sophisticated interaction network. using single-molecule force spectroscopy we structurally localize and quantify these interactions changing on k(+)-dependent transport activation and substrate-binding. k(+) significantly strengthened all interactions ...201121987793
[blood culture results in specialistic unit for pulmonary illness and tuberculosis in bystra in 2008-2010].the study presents the results of analysis of blood culture examination carried out in specialistyczny zespół chorób płuc i gruźlicy in bystra in 2008-2010. contamination incidence and number of blood samples taken from individual patients make it possible to assess if the procedure for taking material for microbiological examination is carried out. the analysis of positive test results allows for the evaluation of epidemiological condition of the hospital and it is also largely dependent on the ...201122184946
tuberculosis-like pneumonias by the aerobic actinomycetes rhodococcus, tsukamurella and gordonia.the order actinomycetales includes phylogenetically diverse but morphologically similar aerobic and anaerobic organisms, exhibiting filamentous branching structures which fragment into rods or coccoid forms. lung pathogens of the order comprise mycobacterium, nocardia, corynebacterium, actinomyces, kytococcus, rothia, williamsia, as well as gordonia, tsukamurella and rhodococcus. particularly, members of the last three genera are uncommon aerobic agents of lung cavitations and tuberculosis(tb)-l ...201122192786
[monoclonal antibodies labeled with colloidal gold for immunochromatographic express analysis of diphtheria toxin].one-step rapid immunochromatographic method for detection of diphtheria toxin in different water samples (phosphate buffer, milk, human nasopharyngeal swab) with the conjugate of monoclonal antibodies labeled with colloidal gold was developed. the limit of visible detection of the diphtheria toxin is 10 ng/ml and 15 min time analysis. the use of silver sensitivity enhancement and scanning equipment decreased the detection limit to 1.25 ng/ml.201121899052
characterization of novel plasmid p1b146 from corynebacterium tuberculostearicum.corynebacterium tuberculostearicum b146, a strain derived from healthy human skin, contains a medium copy plasmid, p1b146. this plasmid was cloned and its complete nucleotide sequence determined. as a result, p1b146 was found to be 4.2 kb in size with a 53% g+c content, plus six open reading frames (orfs) were distinguished. according to a computer-assisted alignment, two of the orfs exhibited significant similarities to already-known common plasmid proteins, the first being the repa gene, respo ...201121876368
Complete genome sequence of Corynebacterium variabile DSM 44702 isolated from the surface of smear-ripened cheeses and insights into cheese ripening and flavor generation.Corynebacterium variabile is part of the complex microflora on the surface of smear-ripened cheeses and contributes to the development of flavor and textural properties during cheese ripening. Still little is known about the metabolic processes and microbial interactions during the production of smear-ripened cheeses. Therefore, the gene repertoire contributing to the lifestyle of the cheese isolate C. variabile DSM 44702 was deduced from the complete genome sequence to get a better understandin ...201122053731
structural view of the regulatory subunit of aspartate kinase from mycobacterium tuberculosis.the aspartate kinase (ak) from mycobacterium tuberculosis (mtb) catalyzes the biosynthesis of aspartate family amino acids, including lysine, threonine, isoleucine and methionine. we determined the crystal structures of the regulatory subunit of aspartate kinase from mtb alone (referred to as mtbakβ) and in complex with threonine (referred to as mtbakβ-thr) at resolutions of 2.6 å and 2.0 å, respectively. mtbakβ is composed of two perpendicular non-equivalent act domains [aspartate kinase, chori ...201121976064
the effect of including lactobacillus reuteri kub-ac5 during post-hatch feeding on the growth and ileum microbiota of broiler chickens.the probiotic strain lactobacillus reuteri kub-ac5, which was originally isolated from chicken intestine, was fed to newborn broiler chicks for the first week post-hatch. the growth and ileum microbiota of the chickens were carefully monitored for 6 wk. the inclusion of 5 log cfu/g of feed statistically increased the bw gain in the first week compared with that of the control group, but this effect did not continue thereafter. significant effects on host feed consumption and the feed-to-growth c ...201122080014
arabitol metabolism of corynebacterium glutamicum and its regulation by atlr.expression profiling of corynebacterium glutamicum in comparison to a derivative deficient of the transcriptional regulator atlr (previously known as sugr or mtlr) revealed eight genes showing more than fourfold higher mrna levels in the mutant. four of these are located in direct vicinity of the atlr gene, i.e., xylb, rbtt, mtld, and sixa, annotated as encoding xylulokinase, ribitol transporter, mannitol 2-dehydrogenase, and phosphohistidine phosphatase, respectively. transcriptional analysis i ...201122178972
host associated polymorphisms in the corynebacterium pseudotuberculosis rpob gene sequence.corynebacterium pseudotuberculosis is a widespread facultative intracellular pathogen that causes caseous lymphadenitis disease in sheep and goats, and generates cutaneous abscesses and granulomas in horses and cattle. although some genes have been studied for diagnostic and phylogenetic analysis within the genus corynebacterium, at subspecies level the pathogen has been poorly analyzed. the aim of this study was to characterize c. pseudotuberculosis strains isolated from domestic animals, throu ...201121482046
targeted allele replacement mutagenesis of corynebacterium pseudotuberculosis.a two-step allele replacement mutagenesis procedure, using a conditionally replicating plasmid, was developed to allow the creation of targeted, marker-free mutations in corynebacterium pseudotuberculosis. the relationship between homologous sequence length and recombination frequency was determined, and enhanced plasmid excision was observed due to the rolling-circle replication of the mutagenesis vector. furthermore, an antibiotic enrichment procedure was applied to improve the recovery of mut ...201121421779
metabolic engineering of 1,2-propanediol pathways in corynebacterium glutamicum.we analyzed 1,2-propanediol (1,2-pd) production in metabolically engineered corynebacterium glutamicum. wild-type c. glutamicum produced 93 µm 1,2-pd after 132 h incubation under aerobic conditions. no gene encoding the methylglyoxal synthase (mgs) which catalyzes the first step of 1,2-pd synthesis from the glycolytic pathway was detected on the c. glutamicum genome, but several genes annotated as encoding putative aldo-keto reductases (akrs) were present. akr functions as a methylglyoxal reduct ...201121424269
corynebacterium glutamicum tailored for efficient isobutanol production.we recently engineered corynebacterium glutamicum for aerobic production of 2-ketoisovalerate by inactivation of the pyruvate dehydrogenase complex, pyruvate:quinone oxidoreductase, transaminase b, and additional overexpression of the ilvbncd genes, encoding acetohydroxyacid synthase, acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. based on this strain, we engineered c. glutamicum for the production of isobutanol from glucose under oxygen deprivation conditions by inactivation ...201121441331
simple dual-spotting procedure enhances nlc-maldi ms/ms analysis of digests with less specific enzymes.the beneficial effect of high mass accuracy in mass spectrometry is especially pronounced when using less specific enzymes as the number of theoretically possible peptides increases dramatically without any cleavage specificity defined. together with a preceding chromatographic separation, high-resolution mass spectrometers such as the maldi-ltq-orbitrap are therefore well suited for the analysis of protein digests with less specific enzymes. a combination with fast, automated, and informative m ...201121480673
identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in corynebacterium glutamicum.corynebacterium glutamicum uses the phosphoenolpyruvate-dependent sugar phosphotransferase system (pts) to uptake and phosphorylate glucose; no other route has yet been identified. disruption of the ptsh gene in wild-type c. glutamicum resulted, as expected, in a phenotype exhibiting little growth on any of the pts sugars: glucose, fructose, and sucrose. however, a suppressor mutant that grew on glucose but not on the other two sugars was spontaneously isolated from the pts-negative strain wt?pt ...201121452034
mixed glucose and lactate uptake by corynebacterium glutamicum through metabolic engineering.the corynebacterium glutamicum atcc 13032 lysc(fbr) strain was engineered to grow fast on racemic mixtures of lactate and to secrete lysine during growth on lactate as well as on mixtures of lactate and glucose. the wild-type c. glutamicum only grows well on l-lactate. overexpression of d-lactate dehydrogenase (dld) achieved by exchanging the native promoter of the dld gene for the stronger promoter of the sod gene encoding superoxide dismutase in c. glutamicum resulted in a duplication of bioma ...201121370474
bronchoalveolar lavage in the diagnosis of ventilator-associated pneumonia: to quantitate or not, that is the question.quantitative bronchoalveolar lavage (bal) is used to diagnose ventilator-associated pneumonia (vap). we prospectively compared semiquantitative (sq) and quantitative (qu) culture of bal for vap diagnosis. ventilated patients suspected of vap underwent bronchoscopic bal. bal fluid was examined by both qu (colony-forming units [cfus]/ml) and sq culture (none, sparse, moderate, or heavy) and results were compared. vap was defined as 105 cfu/ml or greater on qu culture. over 36 months, 319 bals were ...201121375840
substrate specificity and ion coupling in the na+/betaine symporter betp.betp is an na(+)-coupled betaine-specific transporter of the betaine-choline-carnitine (bcc) transporter family involved in the response to hyperosmotic stress. the crystal structure of betp revealed an overall fold of two inverted structurally related repeats (leut-fold) that betp shares with other sequence-unrelated na(+)-coupled symporters. numerous structures of leut-fold transporters in distinct conformational states have contributed substantially to our understanding of the alternating acc ...201121364531
variability and diversity of nasopharyngeal microbiota in children: a metagenomic analysis.the nasopharynx is the ecological niche for many commensal bacteria and for potential respiratory or invasive pathogens like streptococcus pneumoniae, haemophilus influenzae, and neisseria meningitidis. disturbance of a balanced nasopharyngeal (np) microbiome might be involved in the onset of symptomatic infections with these pathogens, which occurs primarily in fall and winter. it is unknown whether seasonal infection patterns are associated with concomitant changes in np microbiota. as young c ...201121386965
cutaneous diphtheria in the urban poor population of vancouver, british columbia: a 10-year review.between 1998 - 2007, records from 33 patients with cutaneous diphtheria from vancouver's inner-city were reviewed. cases were associated with injection drug use and poverty. co-infections with staphylococcus aureus, streptococcus pyogenes and arcanobacterium haemolyticum occurred. corynebacterium diphtheriae is endemic in vancouver's urban core, with mlst sequence type 76 strains predominating.201121525220
exogenous coproporphyrin iii production by corynebacterium aurimucosum and microbacterium oxydans in erythrasma lesions.erythrasma is a superficial skin disease caused by gram-positive corynebacterium species. coral-red fluorescence under wood's light, strongly suggestive of erythrasma, can be attributed to the presence of porphyrins. fractionated porphyrin analysis in erythrasma lesions is yet to be reported. we attempted to investigate erythrasma lesions by isolating the responsible bacteria and determining their exogenous porphyrin production by hplc analysis. we observed a 78-year-old woman with erythrasma wh ...201121393451
Refresh
firstprev
12345678910...
nextlast
Displaying items 1 - 100 of 11012