Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
cow teat skin, a potential source of diverse microbial populations for cheese production.the diversity of the microbial community on cow teat skin was evaluated using a culture-dependent method based on the use of different dairy-specific media, followed by the identification of isolates by 16s rrna gene sequencing. this was combined with a direct molecular approach by cloning and 16s rrna gene sequencing. this study highlighted the large diversity of the bacterial community that may be found on teat skin, where 79.8% of clones corresponded to various unidentified species as well as ...201222081572
aerococcus urinae and trimethoprim-sulfamethoxazole.aerococcus urinae has been described as resistant to trimethoprim-sulfamethoxazole (sxt), but the test medium may affect this observation. twenty-seven clinical isolates of a. urinae tested susceptible to sxt in cation-adjusted mueller-hinton broth (camhb) plus lysed horse blood and resistant in camhb plus lysed sheep blood.201121918023
Which aerococcus? comment on: an unusual microorganism, aerococcus viridans, causing endocarditis and aortic valvular obstruction due to a huge vegetation (turk kardiyol dern ars 2011;39:317-9). 201121983782
an unusual microorganism, aerococcus viridans, causing endocarditis and aortic valvular obstruction due to a huge vegetation.aerococcus viridans is not a common pathogen, and endocarditis due to a. viridans is very rare. a 44-year-old woman with persistent atrial fibrillation and rheumatic valvular heart disease was admitted with fever, sweating, weakness, and progressive shortness of breath. transthoracic echocardiography (tte) demonstrated a 8x9-mm vegetation attached to the right coronary cusp of the aortic valve, causing aortic obstruction. blood cultures yielded a. viridans susceptible to penicillin. despite opti ...201121646834
[aerococcus urinae infective endocarditis.] 201121794855
clinical significance and antimicrobial susceptibilities of aerococcus sanguinicola and aerococcus urinae.a retrospective chart review was performed on 92 patients from whom 118 isolates of aerococcus sanguinicola (n = 52) or aerococcus urinae (n = 66) were obtained from urine cultures between october 2007 and june 2008 to assess clinical presentation and antimicrobial susceptibilities. the mean patient age was 82 (range 24-101) years. the majority was female (76% and 87% for a. sanguinicola and a. urinae, respectively) and institutionalized (61% and 60%, respectively). the majority of male patients ...201121767701
phenotypic characterisation and 16s rrna sequence analysis of veterinary isolates of streptococcus pluranimalium.forty-two isolates of streptococcus pluranimalium were identified from cattle (n=38), sheep (n=2), an alpaca (n=1) and a pheasant (n=1) in the united kingdom. the isolates were confirmed as s.pluranimalium by 16s rrna sequence analysis but could not be differentiated reliably from streptococcus acidominimus by phenotypic characterisation using commercial kits routinely used in veterinary laboratories. the alanyl-phenylalanyl-proline arylamidase reaction could be used to differentiate s. pluranim ...201121703886
culture-negative infective endocarditis of the aortic valve due to aerococcus urinae: a rare aetiology.bacteria of the species aerococcus urinae are gram-positive, catalase-negative cocci that are arranged in pairs, tetrads, or clusters resembling enterococci or staphylococci. they are rare causative agents of infective endocarditis. repetitive urinary tract infections based upon underlying genitourinary tract abnormalities could involve these bacteria. due to their similarity to other gram-positive cocci misinterpretation may occur along the line of microbiologic differentiation, which could pot ...201121889909
Successful treatment of Aerococcus viridans endocarditis in a patient allergic to penicillin.Aerococcus viridans is a rare human pathogen that occasionally causes endocarditis. Most of the reported cases of endocarditis have been treated with penicillin. Here we describe a patient who was allergic to penicillin and was successfully treated with cefotaxime.201122154998
isolation and characterisation of bacterial colonies from seeds and in vitro cultures of fraxinus spp. from italian sites.culturable bacteria were isolated from seeds, embryos and contaminated in vitro cultures of ash (fraxinus excelsior l., f. ornus l. and f. angustifolia l.) and were identified using morphological and molecular analyses. fourteen morphologically distinct isolates were recovered from seeds of fraxinus spp. 16s rdna sequencing categorised these isolates into ten separate genera. three strains isolated from contaminated in vitro cultures, pantoea agglomerans, staphylococcus succinus and aerococcus v ...201121143738
diseases of american lobsters (homarus americanus): a review.the american lobster fishery is a significant economic driver in coastal communities of north america. increasingly, the impacts of infectious disease are recognized as important components and factors in the population ecology and subsequent management of the lobster fishery. both environmental and anthropogenic factors impact marine diseases. the review herein highlights aspects of several important bacterial, fungal and protistan diseases, including gaffkemia, shell disease, vibriosis, diseas ...201121215356
two novel antimicrobial peptides, arasin-likesp and grpsp, from the mud crab scylla paramamosain, exhibit the activity against some crustacean pathogenic bacteria.antimicrobial peptides (amps) are some of the important host molecules required to resist pathogen infection. two novel amps (arasin-likesp and grpsp) were identified from the hemocytes of the mud crab, scylla paramamosain, by analysis of a hemocyte expressed sequence tag library. the deduced open reading frames of the arasin-likesp and grpsp cdnas are 198 and 168 bp, and encode for predicted peptides of 65 and 55 amino acid residues, respectively. the calculated molecular mass of the mature pep ...201121220028
evaluation of microorganisms cultured from injured and repressed tissue regeneration sites in endangered giant aquatic ozark hellbender salamanders.investigation into the causes underlying the rapid, global amphibian decline provides critical insight into the effects of changing ecosystems. hypothesized and confirmed links between amphibian declines, disease, and environmental changes are increasingly represented in published literature. however, there are few long-term amphibian studies that include data on population size, abnormality/injury rates, disease, and habitat variables to adequately assess changes through time. we cultured and i ...201122205979
aerococcus viridans expression of cpn60 is associated with virulence during infection of the american lobster, homarus americanus milne edwards.the gram-positive bacterium aerococcus viridans var. homari is a well-documented causative agent of the lethal systemic disease gaffkemia in both the american lobster, homarus americanus, and the european lobster, homarus gammarus. previous phenotypic characterization has been unsuccessful at differentiating avirulent from virulent strains without performing lethal animal infection trials. recent genetic characterization of a. viridans strains through 16s rrna sequencing and random amplification ...201121988355
Clinical and microbiological features of bacteraemia with Aerococcus urinae.Clin Microbiol Infect ABSTRACT: Aerococcus urinae is a Gram-positive bacterium that can cause invasive infection, including infectious endocarditis (IE), mainly in older men. A. urinae is often misclassified in routine diagnostic laboratories. Through searches in the laboratory databases we identify 16 isolates of A. urinae causing bacteraemia during a 6-year period in southern Sweden, indicating that bacteraemia with A. urinae occurs in at least three cases per million inhabitants per year. The ...201121895858
culture-independent exploration of the teat apex microbiota of dairy cows reveals a wide bacterial species diversity.due to their close proximity to the mammary gland tissue, the bacterial communities lining the teat apex of the udders from lactating cows influence udder health. denaturing gradient gel electrophoresis of the amplified v3 variable region of the 16s rrna gene was used as a culture-independent method to reveal the bacterial composition of 48 samples originating from the teat apices of twelve friesian-holstein dairy cows suffering from clinical mastitis in one quarter. the microbiota belonged to f ...201122266158
[a case of infective aerococcus urinae endocarditis successfully treated by aortic valve replacement].aerococcus urinae is a endocarditis rare causative organism with low virulene. we report an a. urinae endocarditis case treated by aortic valve replacement. an 80-year-old woman hospitalized for urinary tract infection and hydronephrosis due to three-week renal calculi. blood culture on admission isolated streptococcus acidominimus. during the course, she was transferred to our care for surgical intervention after developing congestive heart failure due to severe aortic regurgitation. echocardio ...201122250461
[chlorate reduction by immobilised bacteria in continuous conditions].chlorate reduction by the strain aerococcus dechloraticans tgs-463 immobilized on the corncob under flow conditions has been studied. it has been established that under growth in the medium with chlorate the increase of the dilution rate (d) results in the lower efficacy of the process, i.e., a higher concentration of residual chlorates in the medium and a lower rate of chlorate reduction. the optimal d for chlorate reduction ranges from 1.12 to 1.5 hour(-1).201020364714
aerococcus urinae and aerococcus sanguinicola, two frequently misidentified uropathogens.species belonging to the aerococcus genus are isolated from the urine and blood of elderly patients suffering from urinary tract infections (utis). however, the clinical significance, phenotypic features and antimicrobial susceptibilities of these underestimated and/or misidentified species remain unclear. from march 2006 to november 2008, among 350 non-enterococcal streptococcaceae species isolated from urinary specimens, 30 (8.6%) aerococcus spp. strains were recovered. all strains were charac ...201020482457
aerococcus viridans urinary tract infection in a pediatric patient with secondary pseudohypoaldosteronism.aerococcus viridans is a catalase-negative gram-positive bacterium rarely found as human pathogen. some cases of urinary tract infection (uti) have been described in immunocompromised adults. in this article we describe a uti case caused by this agent in a child with severe obstructive uropathy, clinically presented with secondary pseudohypoaldosteronism (spha). although a. viridans is rarely associated with child infection, it can be responsible for life threatening conditions/ situations. to o ...201021229195
a case report of successful management of aerococcus urinae peritonitis in a patient on peritoneal dialysis. 201021148062
expression of antimicrobial peptides from hyas araneus haemocytes following bacterial challenge in vitro.circulating haemocytes play major roles in the host defense reactions of decapods, including the synthesis and release of antimicrobial peptides (amps). unlike the amps from insects, those in decapods are constitutively expressed. this study aims to establish primary cell cultures of the three main haemocyte types in hyas araneus haemocytes, and to measure the in vitro expression of amp genes in the cells following microbial challenge. the haemocyte populations were separated on percoll gradient ...201020083137
molecular analysis of bacterial population structure and dynamics during cold storage of untreated and treated milk.spoilage bacteria in milk are controlled by treatments such as thermization, microfiltration and addition of carbon dioxide. however, little information is known about the changes in microbial communities during subsequent cold storage of treated milk. culture-dependent methods and a direct molecular approach combining 16s rrna gene clone libraries and quantitative pcr (q-pcr) were applied to obtain a better overview of the structure and the dynamics of milk microbiota. raw milk samples were tre ...201020137820
a case of endocarditis due to aerococcus urinae. 201020369517
[post-operative bacteremia caused by multidrug-resistant aerococcus viridans in a patient with gall bladder cancer].aerococcus viridans is a gram-positive, catalase and oxidase negative, microaerophylic and non-motile coccus which is rarely associated with human infections such as endocarditis, meningitis, artritis and bacteremia. we report a case of bacteremia due to a. viridans in a 61-years-old man with malignant gall bladder neoplasm. the patient underwent a surgical operation and on the 5th day of operation he had severe abdominal pain, vomiting, high fever and discharge from operation site. he was trans ...201020455408
investigation of mineral water springs of miercurea ciuc (csíkszereda) region (romania) with cultivation-dependent microbiological methods.water samples of ten mineral water springs at miercurea ciuc (csíkszereda) region (romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. the results of standard hygienic bacteriological tests showed that the hargita spring had perfect and five other springs had microbiologically acceptable water quality (zsögöd-, nagy-borvíz-, taploca-, szentegyháza- and lobogó springs). the water of borsáros spring was exceptionable (high germ count, presence of enterococc ...201020587384
rapid paper disk test for identification of helicobacter pylori in mixed cultures of gerbil gastric homogenates.a method denominated rapid paper disk test (rpdt) was developed to identify h. pylori colonies in complex cultures obtained from gerbil gastric homogenates. identification is based on a characteristic reaction pattern (rp) for h. pylori colonies given by the combination of the urease-oxidase activities on a paper disk. compared to the rps obtained from gerbil's intestinal tract isolated bacteria, h. pylori rp is completely distinguishable, even from those of bacteria that share one or both activ ...201020624429
aerococcus urinae: severe and fatal bloodstream infections and endocarditis.aerococcus urinae is a pathogen that rarely causes severe or fatal infections. we describe four cases of severe a. urinae bloodstream infections. all patients had underlying urologic conditions. urine cultures, however, were negative.201020660216
microorganisms associated with maari, a baobab seed fermented product.a microbiological study was carried out on baobab fermented seeds (maari) obtained from 4 different production sites in burkina faso (mansila, toulfé, ouagadougou and gorgadji). a total of 390 representative isolates comprising 251 aerobic mesophilic bacteria (amb) and 139 lactic acid bacteria (lab) were isolated and identified to species level using a combination of pheno- and genotypic methods including conventional morphological analysis, carbohydrate fermentation profiling, rep-pcr ((gtg)(5) ...201020688411
platelet activation and biofilm formation by aerococcus urinae, an endocarditis-causing pathogen.the gram-positive bacterium aerococcus urinae can cause infectious endocarditis (ie) in older persons. biofilm formation and platelet aggregation are believed to contribute to bacterial virulence in ie. five a. urinae isolates from human blood were shown to form biofilms in vitro, and biofilm formation was enhanced by the presence of human plasma. four of the a. urinae isolates caused platelet aggregation in platelet-rich plasma from healthy donors. the au3 isolate, which induced platelet aggreg ...201020696834
detection of airborne bacteria in a german turkey house by cultivation-based and molecular methods.today's large-scale poultry production with densely stocked and enclosed production buildings is often accompanied by very high concentrations of airborne microorganisms leading to a clear health hazard for employees working in such environments. depending on the expected exposure to microorganisms, work has to be performed under occupational safety conditions. in this study, turkey houses bioaerosols were investigated by cultivation-based and molecular methods in parallel to determine the conce ...201020720091
molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis.bacterial vaginosis (bv) is an ecological disorder of the vaginal microbiota that affects millions of women annually, and is associated with numerous adverse health outcomes including pre-term birth and the acquisition of sexually transmitted infections. however, little is known about the overall structure and composition of vaginal microbial communities; most of the earlier studies focused on predominant vaginal bacteria in the process of bv. in the present study, the diversity and richness of ...201020819230
molecular cloning, genomic organization and antibacterial activity of a second isoform of antilipopolysaccharide factor (alf) from the mud crab, scylla paramamosain.antimicrobial peptides (amps) serve a major role in host defense systems against microbial invasion. in this study, a novel isoform (alfsp2) of antilipopolysaccharide factors (alfs) was cloned from the mud crab, scylla paramamosain. the open reading frame of the alfsp2 cdna is 348 bp and encodes for a predicted 115 amino acid residues (12.92 kda), and a mature protein of 94 amino acids and a molecular mass of 10.79 kda. the amino acid sequence of alfsp2 has an overall similarity of 74%, 66% and ...201020883796
streptococcus spp. and related bacteria: their identification and their pathogenic potential for chronic mastitis - a molecular approach.streptococcus spp. and related bacteria form a large group of organisms which are associated with bovine intramammary infections (imi). some of them are the well-known mastitis pathogens streptococcus uberis and streptococcus agalactiae. in addition, there are a considerable number of these gram-positive, catalase-negative cocci (pnc) with unclear mastitic pathogenicity such as aerococcus viridans which make the conventional diagnostics of pnc difficult. one diagnostic, api 20 strep (api, biomér ...201020971488
first molecular characterization of fluoroquinolone resistance in aerococcus spp. 201021078934
association of streptococcus pluranimalium with valvular endocarditis and septicaemia in adult broiler parents.the genus streptococcus consists of more than 60 species, but only streptococcus equi subspecies zooepidemicus, streptococcus gallolyticus ssp. gallolyticus, streptococcus gallinaceus, streptococcus dysgalactiae, streptococcus mutans and streptococcus suis have been isolated from poultry. during investigations of the aetiology of increased mortality in broiler parent stock at the end of production, pure cultures of streptococcal-like organisms that could not be classified among these six species ...200919322715
[bacterial diversity recovered from qiyi glacier and runoff, qilian mts].abundance and species diversity of bacteria were investigated respectively by epifluorescence microscope, the culture method and the analysis of 16s rdna genes, with snow and runoff samples from qiyi glacier in the qilian mts. according to the results, the total microbial cells and bacterial cfu range from 10(3) to 10(5) cells x ml(-1) and 0-600 cfu x ml(-1), respectively. the 16s rdna gene of 22 bacterial isolates recovered from snow and runoff samples belong to following groups: bacteroidetes, ...200919927833
[microbal reduction of chlorine oxyanions using plant raw material].a possibility of chlorates reduction at the joint growing of chlorate-reducing and cellulose-degrading bacteria on the medium with paper as a carbon source was proved. the growth rate of associative culture on the medium with paper was by 18-25% lower than that on mpb. enrichment culture of cellulose-degrading bacteria can reduce up to 10% of added chlorates. ethanol is the cellulose hydrolysis product which is primarily consumed under chlorate reduction; then follows the consumption of acetic a ...200919938597
bacterial taxa associated with the hematophagous mite dermanyssus gallinae detected by 16s rrna pcr amplification and ttge fingerprinting.dermanyssus gallinae (arthropoda, mesostigmata) is suspected to be involved in the transmission of a wide variety of pathogens, but nothing is known about its associated non-pathogenic bacterial community. to address this question, we examined the composition of bacterial communities in d. gallinae collected from standard poultry farms in brittany, france. genetic fingerprints of bacterial communities were generated by temporal temperature gradient gel electrophoresis (ttge) separation of indivi ...200919027065
prevalence of sulfonamide resistance genes in bacterial isolates from manured agricultural soils and pig slurry in the united kingdom.the prevalences of three sulfonamide resistance genes, sul1, sul2, and sul3 and sulfachloropyridazine (scp) resistance were determined in bacteria isolated from manured agricultural clay soils and slurry samples in the united kingdom over a 2-year period. slurry from tylosin-fed pigs amended with scp and oxytetracycline was used for manuring. isolates positive for sul genes were further screened for the presence of class 1 and 2 integrons. phenotypic resistance to scp was significantly higher in ...200919064898
[effect of sewage components of match production on chlorate-reducing activity of aerococcus dechloraticans tgs-463].a mathematical model has been offered which describes adequately the process of chlorates reduction by aerococcus dechloraticans tgs-463 culture, the component of sewage from production of ignition mixtures being available in the medium. it is shown that chromates, trivalent iron and zinc take the most negative effect on the rate of chlorates utilization (by the degree of decrease). phosphates have no effect on chlorate-reduction.200920458930
extracorporeal support in a patient with cardiogenic shock due to aerococcus urinae endocarditis.aerococcus urinae is a newly identified gram-positive coccus that causes serious infections. to date, only 15 cases of a. urinae infective endocarditis have been reported, but with a very high mortality. the case is reported of a patient with a. urinae double valve endocarditis. following aortic and mitral valve replacement, the patient suffered from refractory cardiogenic shock; extracorporeal membrane oxygenation was used successfully as a rescue mechanical support.200919852146
[aerococcus urinae endocarditis: first case report in belgium and review of the literature].aerococcus urinae is a rarely reported pathogen, possibly due to difficulties in the identification of the organism. only sixteen cases of a. urinae endocarditis have yet been described. generally, patients were males with predisposing conditions and the outcomes were bad. we describe the first belgian case of endocarditis due to a. urinae. which occurred to a 79-year-old female patient. the diagnostic of a. urinae endocarditis was based on the cardiac ultrasonography and the isolation of the ba ...200819202713
three cases of serious infection caused by aerococcus urinae: a patient with spontaneous bacterial peritonitis and two patients with bacteremia. 200818463786
six cases of aerococcus sanguinicola infection: clinical relevance and bacterial identification.aerococcus sanguinicola is a gram-positive coccus first described in 2001. infections in humans are rare but the use of 16s rrna gene sequencing and improved phenotypic methods has facilitated the identification of a. sanguinicola. we report here 6 cases of a. sanguinicola bacteraemia, 2 of which were associated with infective endocarditis. most patients were elderly (median age 70 y) and had underlying neurological disorders including dementia, cerebral degeneration, and myelomeningocele. the p ...200819086249
isolation and characterisation of two antimicrobial peptides from haemocytes of the american lobster homarus americanus.two antimicrobial peptides from haemocytes of the american lobster, homarus americanus h. milne edwards 1837, were isolated and partially characterised - the first such description for this species. cap-2, an approximately 12kda peptide, contained amino acid sequences corresponding to the predicted sequence for hoa-crustin. crustins are whey acidic protein (wap) domain - containing peptides isolated from crustacean haemocytes. cap-2 did not have any activity towards the gram positive coccus aero ...200818499476
a quantitative approach to the effectiveness of ozone against microbiota organisms colonizing toothbrushes.toothbrushes are rapidly contaminated with different microorganisms, which colonize the oral cavity and interdental spaces. this can represent a possible cause of infection or reinfection. in this study, the ozone experimental effect upon toothbrushes microflora was estimated microbiologically before and after saturation with ozone gas.200818502558
acute pyelonephritis caused by aerococcus urinae in a 12-year-old boy.aerococcus urinae are gram-positive cocci that cause urinary tract infections in adults with underlying genitourinary (gu) tract disease. we report a case of pyelonephritis caused by a. urinae in a 12-year-old boy with a history of pyeloplasty and gu reflux.200818574434
fatal infective endocarditis due to aerococcus urinae--case report and review of literature.aerococcus-related infective endocarditis is a rare form of endocarditis with a high mortality. most cases have associated genitourinary system pathology. aerococcus urinae is a gram-positive coccus that is arranged in clusters resembling staphylococcus, but its growth characteristics and colonial morphology are closer to that of an alpha-hemolytic streptococcus. this organism may initially be dismissed as a contaminant in clinical cultures from nonsterile sites. we report a representative case ...200818692408
aerococcus urinae in bovine reproductive disease. 200818931362
spondylodiscitis due to aerococcus viridans.aerococcus viridans is a microaerophilic, gram-positive, catalase-negative coccus, found singly or in tetrads. to date, no case of spondylodiscitis due to this organism has been reported. we report what we believe to be the first case of spondylodiscitis caused by a. viridans, in a patient with decompensated liver failure, and discuss the possible pathogenesis of this rather uncommon pathogen in this case.200818349378
x-ray structures of aerococcus viridans lactate oxidase and its complex with d-lactate at ph 4.5 show an alpha-hydroxyacid oxidation mechanism.l-lactate oxidase (lox) belongs to a family of flavin mononucleotide (fmn)-dependent alpha-hydroxy acid-oxidizing enzymes. previously, the crystal structure of lox (ph 8.0) from aerococcus viridans was solved, revealing that the active site residues are located around the fmn. here, we solved the crystal structures of the same enzyme at ph 4.5 and its complex with d-lactate at ph 4.5, in an attempt to analyze the intermediate steps. in the complex structure, the d-lactate resides in the substrat ...200818367206
aerococcus urinae in ovine reproductive disease. 200819011254
[protease activity of microflora in the oral cavity of patients with periodontitis].microbial spectrum and non-specific as well as specific iga1 protease activity of isolated microorganisms were investigated in gingival liquid of patients with periodontitis. microorganisms from the gingival liqud of these patients belonged to conditional-pathogenic obligate and facultatively anaerobic bacteria. 24 strains of microorganisms have been identified. nonspecific proteolytic activity was found in the following microorganisms: actinomyces israelii, actinomyces naeslundii, aerococcus vi ...200819205430
[screening and characterization of marine bacteria with antibacterial and cytotoxic activities, and existence of pks i and nrps genes in bioactive strains].antibacterial and cytotoxic activities were screened for marine bacteria which have been isolated from organism, sediments and seawater in china coastal area. the results showed that 42 isolates had antimicrobial activity and 12 isolates had cytotoxicity. molecular phylogenetic analysis of marine bacteria with cytotoxicity based on 16s rrna sequences indicated that they were belong to the genera aerococcus, agrobacterium, alteromona, bacillus, exiguobacterium, paracoccus, pseudoalteromons, rhein ...200717552225
characterization of aerococcus viridans isolates from swine clinical specimens.we present here the biochemical and genetic characterization and antimicrobial susceptibility of 58 isolates of aerococcus viridans isolated in pure culture from different clinical specimens of normally sterile body sites of pigs. a. viridans isolates were commonly susceptible to beta-lactam antimicrobials and exhibited a great genetic heterogeneity as determined by pulsed-field gel electrophoresis typing. the results indicate that a. viridans might be included in the list of possible etiologica ...200717596355
a taxonomic survey of lactic acid bacteria isolated from wheat (triticum durum) kernels and non-conventional flours.in order to explore the correspondence between raw material- and mature sourdough-lactic acid bacterial (lab) communities, 59 italian wheat (triticum durum) grain samples, one bran and six non-conventional flour samples were analyzed through a culture-dependent approach. the highest cell count by an agar medium specific for lab was 2.16 log cfu/g. from about 2300 presumptive lab (gram-positive and catalase-negative) colonies collected, a total of 356 isolates were subjected to identification by ...200717683888
molecular phylogenetic diversity of bacteria and its spatial distribution in composts.to investigate microbial diversity of swine manure composts in the initial stage and the spatial distribution due to gradient effect.200717897238
contamination of milk by enterococci and coliforms from bovine faeces.to determine the contribution of enterococci and coliforms from bovine faeces and teats to contamination of raw milk. methods: putative enterococci (n = 301) and coliforms (n = 365) were isolated from bovine faeces (n = 20), cows' teats (n = 20), the raw milk (n = 1) and the milking environment (n = 4) on one farm. the clonal relationships of each bacterial group were investigated using pulsed-field gel electrophoresis of genomic macrorestriction fragments. representatives of the different clust ...200717953550
serological cross-reactivity of environmental isolates of enterobacter, escherichia, stenotrophomonas, and aerococcus with shigella spp.-specific antisera.using protocols designed for the isolation of shigella from environmental freshwater samples from different regions of bangladesh, 11 bacterial strains giving rise to shigella-like colonies on selective agar plates and showing serological cross-reaction with shigella-specific antisera were isolated. phylogenetic analyses revealed that three of the isolates were most closely related to escherichia coli, four to enterobacter sp., two to stenotrophomonas, and two isolates belonged to the gram-posit ...200717171463
biodiversity of bacterial ecosystems in traditional egyptian domiati cheese.bacterial biodiversity occurring in traditional egyptian soft domiati cheese was studied by pcr-temporal temperature gel electrophoresis (ttge) and pcr-denaturing gradient gel electrophoresis (dgge). bands were identified using a reference species database (j.-c. ogier et al., appl. environ. microbiol. 70:5628-5643, 2004); de novo bands having nonidentified migration patterns were identified by dna sequencing. results reveal a novel bacterial profile and extensive bacterial biodiversity in domia ...200717189434
the structures of pyruvate oxidase from aerococcus viridans with cofactors and with a reaction intermediate reveal the flexibility of the active-site tunnel for catalysis.the crystal structures of pyruvate oxidase from aerococcus viridans (avpox) complexed with flavin adenine dinucleotide (fad), with fad and thiamine diphosphate (thdp) and with fad and the 2-acetyl-thdp intermediate (acthdp) have been determined at 1.6, 1.8 and 1.9 a resolution, respectively. each subunit of the homotetrameric avpox enzyme consists of three domains, as observed in other thdp-dependent enzymes. fad is bound within one subunit in the elongated conformation and with the flavin moiet ...200718007037
changes in immune gene expression and resistance to bacterial infection in lobster (homarus gammarus) post-larval stage vi following acute or chronic exposure to immune stimulating compounds.real-time pcr was used to measure changes in transcript abundance of genes encoding important immune proteins, namely prophenoloxidase (propo gene), beta-1,3-glucan binding protein (betagbp gene) and a 12.2 kda antimicrobial peptide (amp gene) in post-larval stage vi (plvi) juveniles of the european lobster, homarus gammarus. gene expression was studied in both healthy plvi and following single or repeat exposure to a range of compounds claimed to induce immune reactivity. a single acute (3-h) e ...200716569431
improving the thermal stability of lactate oxidase by directed evolution.lactate oxidase is used in biosensors to measure the concentration of lactate in the blood and other body fluids. increasing the thermostability of lactate oxidase can significantly prolong the lifetime of these biosensors. we have previously obtained a variant of lactate oxidase from aerococcus viridans with two mutations (e160g/v198i) that is significantly more thermostable than the wild-type enzyme. here we have attempted to further improve the thermostability of e160g/v198i lactate oxidase u ...200717131051
microbial community structure and dynamics of dark fire-cured tobacco fermentation.the italian toscano cigar production includes a fermentation step that starts when dark fire-cured tobacco leaves are moistened and mixed with ca. 20% prefermented tobacco to form a 500-kg bulk. the dynamics of the process, lasting ca. 18 days, has never been investigated in detail, and limited information is available on microbiota involved. here we show that toscano fermentation is invariably associated with the following: (i) an increase in temperature, ph, and total microbial population; (ii ...200717142368
diversity of lactic acid bacteria associated with fish and the fish farm environment, established by amplified rrna gene restriction analysis.lactic acid bacteria have become a major source of concern for aquaculture in recent decades. in addition to true pathogenic species of worldwide significance, such as streptococcus iniae and lactococcus garvieae, several species have been reported to produce occasional fish mortalities in limited geographic areas, and many unidentifiable or ill-defined isolates are regularly isolated from fish or fish products. to clarify the nature and prevalence of different fish-associated bacteria belonging ...200717337536
quantitative analysis of the intestinal bacterial community in one- to three-week-old commercially reared broiler chickens fed conventional or antibiotic-free vegetable-based diets.to explore the effect of drug-free poultry production on the intestinal microflora of broiler chickens, the bacterial community of this environment was quantitatively profiled in both conventionally reared birds and birds reared without antibiotic growth promotants (agps) on a vegetable-based diet.200717381758
culture-negative infective endocarditis caused by aerococcus urinae.aerococcus urinae is a rarely reported pathogen that often causes mild urinary tract infection (uti), although serious complications such as endocarditis and septicemia have also been described. the organism may easily be missed or misidentified when using commercial detection systems. a. urinae is resistant to sulfonamides and, therefore, a typical treatment for uti may be inappropriate. to date, 14 cases of a. urinae infective endocarditis (ie) have been reported, most of which were elderly ma ...200717484472
infective endocarditis and spondylodiscitis in a patient due to aerococcus urinae: first report. 200716766062
enterococcus and lactobacillus contamination of raw milk in a farm dairy environment.enterococci and lactobacilli are ubiquitously found in the intestinal microflora of humans and animals. the aim of the present study was to determine the importance of bovine faeces as a source of these organisms in raw milk. one hundred and fifty six putative enterococci and 362 lactobacilli were isolated from bovine faeces (n=26), cows' teats, raw milk, the milking machine and the milking environment on one farm. the clonal relationships of each group were investigated using pulsed-field gel e ...200717189657
crystallographic study on the interaction of l-lactate oxidase with pyruvate at 1.9 angstrom resolution.l-lactate oxidase (lox) from aerococcus viridans catalyzes the oxidation of l-lactate to pyruvate by the molecular oxygen and belongs to a large family of 2-hydroxy acid-dependent flavoenzymes. to investigate the interaction of lox with pyruvate in structural details and understand the chemical mechanism of flavin-dependent l-lactate dehydrogenation, the lox-pyruvate complex was crystallized and the crystal structure of the complex has been solved at a resolution of 1.90 angstrom. one pyruvate m ...200717517371
aerococcus suis sp. nov., isolated from clinical specimens from swine.biochemical and molecular genetic studies were performed for five isolates of unknown gram-positive, catalase-negative, cocci-shaped micro-organisms obtained from clinical samples from pigs. the micro-organisms were tentatively identified as aerococcus species on the basis of the results from cellular morphological and biochemical tests. 16s rrna gene sequencing studies confirmed the provisional identification of the isolates as members of the genus aerococcus, but the micro-organism did not cor ...200717551045
case study: aerococcus urinae as pathogen in peritoneal dialysis peritonitis--a first report. 200717984441
[use of immobilized cells of bacteria in the process of purification of waste water containing chlorates and chromates].some regularities of immobilization of chlorate-reducing bacteria by various carriers and especially sewage treatment for chlorate and chromates by adhered bacteria have been studied. most bacterial cells are immobilized during the first hour of contact with the carrier. the studied carriers, as to their ability to adsorb aerococcus dechloraticans tgs-463 cells, may be arranged in the following sequence: porolon > claydite > glass broaches > maize stem > barley straw. the 12-24 hour culture aero ...200616686220
cloning of a crustin-like, single whey-acidic-domain, antibacterial peptide from the haemocytes of the european lobster, homarus gammarus, and its response to infection with bacteria.degenerate pcr was used to isolate a 221-base pair nucleotide sequence of a new crustin-like antibacterial peptide from the haemocytes of the european lobster, homarus gammarus. rapid amplification of cdna ends was used to extend the sequence to determine the complete open reading frame and un-translated regions. the inferred amino acid sequence of this peptide was found to be similar to crustin-like peptides isolated for several species of shrimp as well as the shore crab, carcinus maenas. the ...200616144710
the 2.1 a structure of aerococcus viridans l-lactate oxidase (lox).the crystal structure of l-lactate oxidase (lox) from aerococcus viridans has been determined at 2.1 a resolution. lox catalyzes the flavin mononucleotide (fmn) dependent oxidation of lactate to pyruvate and hydrogen peroxide. lox belongs to the alpha-hydroxy-acid oxidase flavoenzyme family; members of which bind similar substrates and to some extent have conserved catalytic properties and structural motifs. lox crystallized as two tightly packed tetramers in the asymmetric unit, each having fou ...200617142893
microbial colonization of tourniquets used in orthopedic surgery.this study analyzed tourniquets used for orthopedic surgery in our hospital to determine the frequency and type of microbial contamination. group a tourniquets were from our main operating room, group b tourniquets were from our ambulatory surgicenter, group c tourniquets were unused, prepackaged, sterile tourniquets from our main operating room, and group d tourniquets were sterilely packed tourniquets from our ambulatory surgicenter. tourniquets from groups a, b, c, and d had 100%, 40%, 0%, an ...200616924865
the crystal structure of l-lactate oxidase from aerococcus viridans at 2.1a resolution reveals the mechanism of strict substrate recognition.l-lactate oxidase (lox) from aerococcus viridans is a member of the alpha-hydroxyacid-oxidase flavoenzyme family. we have determined the three-dimensional structure of lox and revealed the mechanism of substrate recognition. the lox monomer structure has a typical alpha(8)/beta(8) motif commonly found in other flavin family proteins. a related enzyme, glycolate oxidase, catalyzes the oxidation of glycolate rather than lactate. comparison of the two enzyme structures highlights the importance of ...200617007814
immunogen-dependent quantitative and qualitative differences in phagocytic responses of the circulating hemocytes of the lobster homarus americanus.phagocytic responses in circulating hemocytes of the lobster homarus americanus were measured before and after treatment of lobsters with 2 different immunogens: (1) lipolysaccharide (lps) or endotoxin from a non-pathogenic pseudomonas perolens, and (2) a vancomycin/live gram-positive pathogen (aerococcus viridans [var.] homari) combination, essentially attenuated cells, shown previously to induce a high degree of resistance to this pathogen. the responses elicited by each of the immunogens were ...200616724563
urosepsis with actinobaculum schaalii and aerococcus urinae.actinobaculum was isolated from urine only after prolonged incubation in 5% co(2) after discrepancy between urine gram stain and initial culture results was observed. additional patients were diagnosed using this method. the prevalence of actinobaculum species in urinary tract infections is underestimated since it is not isolated by routine urine culture procedures.200616455938
crystallization and preliminary x-ray diffraction study of l-lactate oxidase (lox), r181m mutant, from aerococcus viridans.l-lactate oxidase (lox) from aerococcus viridans is a member of the alpha-hydroxyacid oxidase flavoenzyme family. an x-ray crystallographic study of a lox mutant in which arg181 is replaced by met was initiated in order to understand the functions of the conserved amino-acid residues around the fmn in the enzyme active site. lox-r181m crystals belong to the tetragonal space group i422, with unit-cell parameters a = b = 192.632, c = 200.263 a, alpha = beta = gamma = 90 degrees. there are four mon ...200516511063
[gram-positive cocci as an opportunistic infection factor].the frequency of opportunistic infections increased in the last years. the opportunistic infections are serious and very difficult to diagnose complication at patients with lower immunity. the following factors are conducive to develop infections caused by opportunistic bacteria: invasive research methods, medical treatments and immunological defects. the publication presents pathogenicity of bacteria of following types: aerococcus, kocuria, kytococcus, lactococcus, leuconostoc, micrococcus, ped ...200516161935
antibacterial and antifungal lysozyme-type activity in cameraria ohridella pupae.lysozyme-type antibacterial and antifungal activity in pupae of cameraria ohridella was studied. activity against micrococcus luteus and bacillus megaterium was detected in pupae extract. also antifungal activity from c. ohridella pupae extract directed against saccharomyces cerevisiae strain w 303 was shown. during immunoblotting two bands in pupae extract, with molecular mass of about 15 and 28 kda were recognized by antibodies directed against hewl. after acid electrophoresis followed by bioa ...200516169556
volatile and biogenic amines, microbiological counts, and bacterial amino acid decarboxylase activity throughout the salt-ripening process of anchovies (engraulis encrasicholus).chemical and microbiological parameters were studied during the industrial production of salt-ripened anchovies (engraulis encrasicholus). gradual acidification and increases in the proteolysis index and in total volatile basic nitrogen were observed. at the end of the maturing process, the values reached ph 5.55 +/- 0.03, 21.33 +/- 5.82%, and 44.06 +/- 12.47 mg/ 100 g, respectively. in the three studied anchovy batches, the biogenic amines tyramine, histamine, putrescine, cadaverine, and agmati ...200521132979
aerococcus urinae: polyphasic characterization of the species.a polyphasic characterization of aerococcus urinae is presented. in this study the intraspecies relationships between 26 strains of varying geographical origin were examined by phenotypic tests, ribotyping and multilocus enzyme electrophoresis. the results demonstrated two main phenotypic patterns that could be distinguished in tests for hydrolysis of aesculin, and acid production from amygdalin and salicin. strains were either negative (n=19) or positive (n=6) in these tests. one strain had a d ...200516086822
effect of capsulation of opportunistic pathogenic bacteria on binding of the pattern recognition molecules mannan-binding lectin, l-ficolin, and h-ficolin.mannan-binding lectin (mbl), l-ficolin, and h-ficolin are pattern recognition molecules of the innate immune system. we investigated their ability to bind to different serotypes and noncapsulated variants of two gram-positive bacterial species, streptococcus pneumoniae and staphylococcus aureus. mbl did not bind to capsulated s. aureus or capsulated s. pneumoniae but did bind to a noncapsulated s. aureus variant (wood). l-ficolin bound to some capsulated s. aureus serotypes (serotypes 1, 8, 9, 1 ...200515664949
a case of aerococcus urinae septicemia: an unusual presentation and severe disease course. 200516117890
clinical significance of aerococcus urinae: a retrospective review.a retrospective chart review was performed on 54 patients with positive urine cultures during a 1-year period to assess the clinical significance of aerococcus urinae. based on predetermined criteria, patients were classified into 2 groups: those with urinary tract infections (utis) and those who were considered colonized. the majority of the patients were > or =65 years old and were female. only 31% of patients with uti and 45% of colonized patients had a. urinae isolated in pure cultures. both ...200516269223
lytic enzyme, labiase for a broad range of gram-positive bacteria and its application to analyze functional dna/rna.the lytic activity of labiase and achromopeptidase for bacterial dna/rna extraction were compared. rapid lysis of many bacterial strains was observed with labiase followed by sds treatment. both labiase and achromopeptidase showed high lytic activity against bacterial strains with the a1alpha chemotype (e.g., aerococcus viridans) and the a3alpha chemotype (e.g., staphylococcus epidermidis) for cell wall peptidoglycan structures. the lytic activity of labiase was higher than that of achromopeptid ...200515722152
molecular cloning and characterization of cdna of penaeidin-like antimicrobial peptide from tiger shrimp (penaeus monodon).we report the molecular cloning and characterization of a penaeidin-like antimicrobial peptide (amp) complementary dna from the hemocytes of tiger shrimp (penaeus monodoni). a tiger shrimp amp cdna containing the entire coding region of the peptide, determined by rapid amplification of cdna 5' and 3' ends and polymerase chain reaction of the messenger rna isolated from hemocytes of the shrimp, is 683 bp in length with an open reading frame of 222 bp. the deduced amino acid sequence of this antim ...200515776314
genetic characterization of the lobster pathogen aerococcus viridans var. homari by 16s rrna gene sequence and rapd.a combination of 16s rrna sequencing and random amplified polymorphic dna (rapd) analysis was used to evaluate the genetic diversity within aerococcus viridans var. homari, the causative agent of gaffkemia in lobsters. a collection of 7 a. viridans var. homari strains and 2 avirulent a. viridans-like cocci isolated from homarid lobsters harvested from different regions on the atlantic coast of north america were analyzed. the isolates are separated geographically and temporally between the years ...200515819439
reclassification of pediococcus urinaeequi (ex mees 1934) garvie 1988 as aerococcus urinaeequi comb. nov.the taxonomic status of pediococcus urinaeequi is described, and the transfer of the species to the genus aerococcus with the name aerococcus urinaeequi comb. nov. is proposed, on the basis of the analysis of 16s rrna gene sequence and dna-dna hybridization data.200515879276
an unusual bacterium, aerococcus viridans, and four cases of infective endocarditis.four cases are described of aerococcus viridans endocarditis with favorable outcome, focusing on the clinical picture. extracardiac findings (e.g. neurological, renal, musculoskeletal, intra-abdominal) in infective endocarditis (ie) have been recognized more frequently in recent years, and may delay the diagnosis and have a negative influence on the prognosis. aerococcus viridans, a very rare microorganism causing invasive infections, has been associated with bacteremia, septic arthritis, and es ...200515974524
identification of streptococcus spp. causing bovine mastitis by pcr-rflp of 16s-23s ribosomal dna.traditional microbiological methods for identification of streptococcus spp. causing bovine mastitis have been demonstrated to be less than highly reliable. pcr-rflp analysis of 16s-23s ribosomal dna was used to characterise seven reference strains of streptococcal mastitis pathogens as well as four reference strains of other gram-positive, catalase-negative cocci of bovine origin to allow comparative identification of field isolates. rflp analysis of pcr products, using a combination of two res ...200516289400
m-ficolin, an innate immune defence molecule, binds patterns of acetyl groups and activates complement.ficolins play a role in the innate immune defence as pathogen-associated molecular pattern recognition molecules. three ficolins are found in humans: h-ficolin, l-ficolin and m-ficolin. l-ficolin and h-ficolin circulate in blood in complexes with mannan-binding lectin-associated serine proteases (masps) and are capable of activating the complement system. l-ficolin shows affinity for acetylated compounds and binds to various capsulated strains of bacteria. h-ficolin has been shown to bind aeroco ...200516305643
induced resistance to infection of lobsters homarus americanus by aerococcus viridans (var.) homari, the bacterium causing gaffkemia.a vaccine composed of steam sterilized (autoclaved) cells of a virulent strain of aerococcus viridans (var.) homari was effective in protecting lobsters homarus americanus against gaffkemia. at 15 degrees c the heat-killed vaccines (hkv) at concentrations between 1 and 5 x 10(7) particles kg(-1) lobster body wt induced maximal protection in induction periods ranging from 7 to 11 d. protection was substantial over the course of a 30 d post-induction trial period. spring-caught lobsters (i.e. thos ...200415672875
septicaemia associated with an aerococcus viridans infection in immunodeficient mice.this report describes a case series of septicaemia caused by infection with aerococcus viridans in immunodeficient nod/ltsz-prkdc(scid) (nod/scid) mice. during a period of 3 weeks more than 40 animals died or became ill with clinical signs of ruffled coat, weight loss, laboured breeding, and distended abdomen. at necropsy it was found that the animals displayed symptoms of sepsis with widespread abscesses in the liver, heart, lungs or pyogenic peritonitis. a gram-positive coccus was isolated in ...200415207044
pcr detection of bacillus and staphylococcus in various foods.a broad-range pcr assay for the detection of bacteria belonging to bacillus and staphylococcus genera was developed. primers targeting the bacterial 16s rrna gene were newly designed and used in a pcr assay. to determine the specificity of the assay, 81 different bacterial strains (of 50 genera), 2 fungi, 3 animals, and 4 plants were tested. results were positive for every tested bacillus, staphylococcus, or aerococcus strain. in addition, the result for listeria grayi was positive with lower pc ...200415222564
l-ficolin is a pattern recognition molecule specific for acetyl groups.l-ficolin and h-ficolin are molecules of the innate immune system. upon recognition of a suitable target they activate the complement system. the ligand recognition structure of ficolins is contained within a fibrinogen-like domain. we examined the selectivity of the ficolins through inhibiting the binding to bacteria or to beads coupled with n-acetylglucosamine. the binding of l-ficolin to streptococcus pneumoniae 11f and the beads was inhibited by n-acetylated sugars and not by non-acetylated ...200415331601
studies on the virulence of aerococcus viridans (var.) homari, the causative agent of gaffkemia, a fatal disease of homarid lobsters.virulent and avirulent strains of aerococcus viridans (var.) homari were used to extend previous studies to determine and confirm differences between the 2 types. virulent strains possessed polysaccharide capsules and were not agglutinated by lobster hemolymph serum; avirulent strains did not have capsules, were agglutinated by the lobster hemolymph serum, and most did not grow well in lobster hemolymph serum. growth of the avirulent strains in sterile lobster hemolymph serum induced the product ...200415460859
Refresh
firstprev
123
nextlast
Displaying items 1 - 100 of 278