Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| enhanced heterologous protein productivity by genome reduction in lactococcus lactis nz9000. | the implementation of novel chassis organisms to be used as microbial cell factories in industrial applications is an intensive research field. lactococcus lactis, which is one of the most extensively studied model organisms, exhibits superior ability to be used as engineered host for fermentation of desirable products. however, few studies have reported about genome reduction of l. lactis as a clean background for functional genomic studies and a model chassis for desirable product fermentation ... | 2017 | 28049473 |
| a perspective study of koumiss microbiome by metagenomics analysis based on single-cell amplification technique. | koumiss is a traditional fermented dairy product and a good source for isolating novel bacteria with biotechnology potential. in the present study, we applied the single-cell amplification technique in the metagenomics analysis of koumiss. this approach aimed at detecting the low-abundant bacteria in the koumiss. briefly, each sample was first serially diluted until reaching the level of approximately 100 cells. then, three diluted bacterial suspensions were randomly picked for further study. by ... | 2017 | 28223973 |
| lactococcus lactis phage tp901-1 as a model for siphoviridae virion assembly. | phages infecting lactococcus lactis pose a serious threat to the dairy fermentation sector. consequently, they are among the most thoroughly characterized gram positive-infecting phages. the majority of lactococcal phages belong to the tailed family of phages named the siphoviridae. the coliphage lambda and the bacillus subtilis phage spp1 have been the predominant comparators for emerging siphophages both genomically and structurally and both phages recognize a membrane protein receptor. in con ... | 2017 | 27144086 |
| inhibition of listeria monocytogenes biofilms by bacteriocin-producing bacteria isolated from mushroom substrate. | this study was designed to investigate the ability of naturally occurring bacteria isolated from mushroom substrate to prevent biofilm formation by listeria monocytogenes or to remove existing biofilms in mushroom production facilities. | 2017 | 27797439 |
| combined chemical and physical transformation method with rbcl and sepiolite for the transformation of various bacterial species. | dna transformation that delivers plasmid dnas into bacterial cells is fundamental in genetic manipulation to engineer and study bacteria. developed transformation methods to date are optimized to specific bacterial species for high efficiency. thus, there is always a demand for simple and species-independent transformation methods. we herein describe the development of a chemico-physical transformation method that combines a rubidium chloride (rbcl)-based chemical method and sepiolite-based phys ... | 2017 | 28185866 |
| a review on lactococcus lactis: from food to factory. | lactococcus lactis has progressed a long way since its discovery and initial use in dairy product fermentation, to its present biotechnological applications in genetic engineering for the production of various recombinant proteins and metabolites that transcends the heterologous species barrier. key desirable features of this gram-positive lactic acid non-colonizing gut bacteria include its generally recognized as safe (gras) status, probiotic properties, the absence of inclusion bodies and endo ... | 2017 | 28376880 |
| the additional effects of a probiotic mix on abdominal adiposity and antioxidant status: a double-blind, randomized trial. | to investigate whether a probiotic mix has additional effects when compared with an isolated dietary intervention on the body composition, lipid profile, endotoxemia, inflammation, and antioxidant profile. | 2017 | 28008750 |
| the use of 16s rrna gene metagenetic monitoring of refrigerated food products for understanding the kinetics of microbial subpopulations at different storage temperatures: the example of white pudding. | in order to control food losses and wastage, monitoring the microbial diversity of food products, during processing and storage is important, as studies have highlighted the metabolic activities of some microorganisms which can lead to spoilage. knowledge of this diversity can be greatly improved by using a metagenetic approach based on high throughput 16s rrna gene sequencing, which enables a much higher resolution than culture-based methods. moreover, the jameson effect, a phenomenon described ... | 2017 | 27751567 |
| filthy lucre: a metagenomic pilot study of microbes found on circulating currency in new york city. | paper currency by its very nature is frequently transferred from one person to another and represents an important medium for human contact with-and potential exchange of-microbes. in this pilot study, we swabbed circulating $1 bills obtained from a new york city bank in february (winter) and june (summer) 2013 and used shotgun metagenomic sequencing to profile the communities found on their surface. using basic culture conditions, we also tested whether viable microbes could be recovered from b ... | 2017 | 28384336 |
| technological properties and biogenic amines production by bacteriocinogenic lactococci and enterococci strains isolated from raw goat's milk. | technological properties and biogenic amine production were analyzed in 56 bacteriocinogenic lactococci and enterococci strains isolated from raw goat's milk. fifteen lactococci strains were able to reduce milk ph to 5.3 or lower after 6 h, while enterococci strains were initially slow in producing acids. lactococcus lactis subsp. lactis glc06 and three strains of enterococcus faecalis (gen20, gen22, and gen23) presented high proteolytic activity. l. lactis subsp. lactis glc06 and e. faecalis ge ... | 2017 | 28221886 |
| some technological properties of lactic acid bacteria isolated from dahi and datshi, naturally fermented milk products of bhutan. | dahi and datshi are common naturally fermented milk (nfm) products of bhutan. population of lactic acid bacteria (lab) in dahi (ph 3.7) and datshi (ph 5.2) was 1.4 × 10(7) and 3.9 × 10(8) cfu/ml, respectively. based on 16s rrna gene sequencing isolates of lab from dahi and datshi were identified as enterococcus faecalis, e. faecium, lactococcus lactis subsp. lactis. lab strains were tested for some technological properties. all lab strains except e. faecalis ch2:17 caused coagulation of milk at ... | 2017 | 28203227 |
| diffusible substances from lactic acid bacterial cultures exert strong inhibitory effects on listeria monocytogenes and salmonella enterica serovar enteritidis in a co-culture model. | food-borne infections cause huge economic and human life losses. listeria monocytogenes and salmonella enterica serovar enteritidis are among the top ranking pathogens causing such losses. control of such infections is hampered by persistent contamination of foods and food-processing environments, resistance of pathogens to sanitizing agents, existence of heterogeneous populations of pathogens (including culturable and viable but non-culturable cells) within the same food items, and inability to ... | 2017 | 28202007 |
| inhibition of listeria monocytogenes on ready-to-eat meats using bacteriocin mixtures based on mode-of-action. | bacteriocin-producing (bac⁺) lactic acid bacteria (lab) comprising selected strains of lactobacillus curvatus, lactococcus lactis, pediococcus acidilactici, and enterococcus faecium and thailandicus were examined for inhibition of listeria monocytogenes during hotdog challenge studies. the bac⁺ strains, or their cell-free supernatants (cfs), were grouped according to mode-of-action (moa) as determined from prior studies. making a mixture of as many moas as possible is a practical way to obtain a ... | 2017 | 28335414 |
| microbiological and biochemical aspects of inland pecorino abruzzese cheese. | little is known on physicochemical and biochemical characteristics of "pecorino" abruzzese cheese in l'aquila province, an artisanal cheese produced from ewe raw full-cream milk. three batches of inland "pecorino" abruzzese cheese were examined for microbiological, compositional, biochemical and sensory characteristics at the aim of isolating and storing in a bacterial collection, indigenous strain to preserve the microbial biodiversity present in this cheese, to a possible definition of a pdo. ... | 2017 | 28280791 |
| enhanced control of listeria monocytogenes by enterococcus faecium ke82, a multiple enterocin-producing strain, in different milk environments. | enterococcus faecium ke82, isolated from traditional greek graviera cheese, was identified in pure broth cultures in vitro as a multiple enterocin-producing bacterial strain possessing the structural enta, entb, and entp enterocin genes. e. faecium ke82 was further assessed for in situ antilisterial activity in raw milk (rm) and commercially thermized milk (tm; 63°c for 30 s) in the presence of the indigenous microbiota and in sterile raw milk (srm; 121°c for 5 min) with or without the addition ... | 2017 | 28221890 |
| growth interactions and antilisterial effects of the bacteriocinogenic lactococcus lactis subsp. cremoris m104 and enterococcus faecium ke82 strains in thermized milk in the presence or absence of a commercial starter culture. | traditional greek cheeses are often produced from thermized milk (tm) with the use of commercial starter cultures (cscs), which may not inhibit growth of listeria monocytogenes completely. therefore, this study evaluated the behavior of an artificial l. monocytogenes contamination in commercially tm (63 °c; 30 s) inoculated with a csc plus lactococcus lactis subsp. lactis m104 and/or enterococcus faecium ke82, two indigenous strains producing nisin a and enterocin a and b, respectively. inoculat ... | 2017 | 28213019 |
| characterization of class iia bacteriocin resistance in enterococcus faecium. | vancomycin-resistant enterococci, particularly resistant enterococcus faecium, pose an escalating threat in nosocomial environments because of their innate resistance to many antibiotics, including vancomycin, a treatment of last resort. many class iia bacteriocins strongly target these enterococci and may offer a potential alternative for the management of this pathogen. however, e. faecium's resistance to these peptides remains relatively uncharacterized. here, we explored the development of r ... | 2017 | 28115354 |
| monitoring of wheat lactic acid bacteria from the field until the first step of dough fermentation. | the present work was carried out to retrieve the origin of lactic acid bacteria (lab) in sourdough. to this purpose, wheat lab were monitored from ear harvest until the first step of fermentation for sourdough development. the influence of the geographical area and variety on lab species/strain composition was also determined. the ears of four triticum durum varieties (duilio, iride, saragolla and simeto) were collected from several fields located within the palermo province (sicily, italy) and ... | 2017 | 27889157 |
| a new broad range plasmid for dna delivery in eukaryotic cells using lactic acid bacteria: in vitro and in vivo assays. | lactococcus lactis is well documented as a promising candidate for development of novel oral live vaccines. it has been broadly engineered for heterologous expression, as well as for plasmid expression vector delivery, directly inside eukaryotic cells, for dna vaccine, or as therapeutic vehicle. this work describes the characteristics of a new plasmid, pexu (extra chromosomal unit), for dna delivery using l. lactis and evaluates its functionality both by in vitro and in vivo assays. this plasmid ... | 2017 | 28344994 |
| an engineered food-grade lactococcus lactis strain for production and delivery of heat-labile enterotoxin b subunit to mucosal sites. | recent researches have been focusing on mucosal immune adjuvants, which play the key roles in mucosal immunization and have become the limitation for non-injected vaccine development. escherichia coli heat-labile enterotoxin b subunit (ltb) was regarded as a promising mucosal adjuvant for its nontoxicity and potent activity. ltb preparation issues have always been recurring, in part owing to that the recombinant ltb expressed by e. coli does not act as its native form. | 2017 | 28264682 |
| turning hydrophilic bacteria into biorenewable hydrophobic material with potential antimicrobial activity via interaction with chitosan. | alteration of a bacteriocin-producing hydrophilic bacterium, lactococcus lactis io-1, into a hydrophobic material with potential antimicrobial activity using chitosan was investigated and compared with five other bacterial species with industrial importance. the negatively charged bacterial cells were neutralized by positively charged chitosan, resulting in a significant increase in the hydrophobicity of the bacterial cell surface. the largest gram-positive b. megaterium atcc 14581 showed a mode ... | 2017 | 28161626 |
| cytoplasmic expression of a thermostable invertase from thermotoga maritima in lactococcus lactis. | to evaluate the secretory and cytoplasmic expression of a thermostable thermogata maritima invertase in lactococcus lactis. | 2017 | 28155177 |
| engineering mycobacteria for the production of self-assembling biopolyesters displaying mycobacterial antigens for use as a tuberculosis vaccine. | tuberculosis (tb) is a disease caused by mycobacterium tuberculosis or mycobacterium bovis and still remains one of the world's biggest global health burdens. recently, engineered polyhydroxyalkanoate (pha) biobeads that were produced in both escherichia coli and lactococcus lactis and displayed mycobacterial antigens were found to induce significant cell-mediated immune responses in mice. we observed that such pha beads contained host cell proteins as impurities, which we hypothesized to have t ... | 2017 | 28087528 |
| quantitative analyses of the bacterial microbiota of rearing environment, tilapia and common carp cultured in earthen ponds and inhibitory activity of its lactic acid bacteria on fish spoilage and pathogenic bacteria. | the present study aimed to evaluate the bacterial load of water, nile tilapia and common carp intestines from earthen ponds, isolate lactic acid bacteria (lab) and assess their antimicrobial activity against fish spoilage and pathogenic bacteria. following enumeration and isolation of microorganisms the antimicrobial activity of the lab isolates was evaluated. taxonomic identification of selected antagonistic lab strains was assessed, followed by partial characterisation of their antimicrobial m ... | 2017 | 28063102 |
| translation-dependent bioassay for amino acid quantification using auxotrophic microbes as biocatalysts of protein synthesis. | bioassay for amino acid quantification is an important technology for a variety of fields, which allows for easy, inexpensive, and high-throughput analyses. here, we describe a novel translation-dependent bioassay for the quantification of amino acids. for this, the gene encoding firefly luciferase was introduced into lactococcus lactis auxotrophic to glu, his, ile, leu, pro, val, and arg. after a preculture where luciferase expression was repressed, the cells were mixed with analytes, synthetic ... | 2017 | 27942758 |
| influence of pdo ragusano cheese biofilm microbiota on flavour compounds formation. | the objectives of the present study were to characterize the biofilm microbiota of 11 different farms (from a to k), producing pdo ragusano cheese, and to investigate on its ability to generate volatile organic compounds (vocs) in milk samples inoculated with biofilm and incubated under ragusano cheese making conditions. the biofilms were subjected to plate counting and pcr/t/dgge analysis and the vocs generated in incubated milk samples were evaluated through smartnose, gc/o, and gc/ms. strepto ... | 2017 | 27697162 |
| immunization with r-lactococcus lactis expressing outer membrane protein a of shigella dysenteriae type-1: evaluation of oral and intranasal route of administration. | to evaluate the comparative immunogenic potential of food grade lactococcus lactis expressing outer membrane protein a (ompa) of shigella dysenteriae type-1 (sd-1) when administered either orally or intranasally. | 2017 | 27860045 |
| insertion of single-chain variable fragment (scfv) peptide linker improves surface display of influenza hemagglutinin (ha1) on non-recombinant lactococcus lactis. | heterologous protein displayed on the surface of lactococcus lactis using the binding domain of n-acetylmuramidase (acma) has a potential application in vaccine delivery. in this study, we developed a non-recombinant l. lactis surface displaying the influenza a (h1n1) 2009 hemagglutinin (ha1). three recombinant proteins, ha1/l/acma, ha1/acma, and ha1 were overexpressed in escherichia coli, and purified. in the binding study using flow cytometry, the ha1/l/acma, which contained the single-chain v ... | 2017 | 27802566 |
| antimicrobial peptide production and purification. | antimicrobial peptides (amps) are natural defense compounds which are synthesized as ribosomal gene-encoded pre-peptides and produced by all living organisms. amps are small peptides, usually cationic and typically have hydrophobic residues which interact with cell membranes and have either a narrow or broad spectrum of biological activity. amps are isolated from the natural host or heterologously expressed in other hosts such as escherichia coli. the proto-typical lantibiotic nisin is a widely ... | 2017 | 27730566 |
| profiling of mycoplasma gallisepticum ribosomes. | the development of high-throughput technologies is increasingly resulting in identification of numerous cases of low correlation between mrna and the protein level in cells. these controversial observations were made on various bacteria, such as e. coli, desulfovibrio vulgaris, and lactococcus lactis. thus, it is important to develop technologies, including high-throughput techniques, aimed at studying gene expression regulation at the level of translation. in the current study, we performed pro ... | 2017 | 26798497 |
| microbial anti-inflammatory molecule (mam) from faecalibacterium prausnitzii shows a protective effect on dnbs and dss-induced colitis model in mice through inhibition of nf-κb pathway. | faecalibacterium prausnitzii and its supernatant showed protective effects in different chemically-induced colitis models in mice. recently, we described 7 peptides found in the f. prausnitzii supernatant, all belonging to a protein called microbial anti-inflammatory molecule (mam). these peptides were able to inhibit nf-κb pathway in vitro and showed anti-inflammatory properties in vivo in a dinitrobenzene sulfate (dnbs)-induced colitis model. in this current proof we tested mam effect on nf-κb ... | 2017 | 28203226 |
| probiotic bacteria inhibit the bovine respiratory pathogen mannheimia haemolytica serotype 1 in vitro. | this study evaluated the potential of probiotic bacteria to inhibit growth and cell adhesion of the bovine respiratory pathogen mannheimia haemoltyica serotype 1. the inhibitory effects of nine probiotic strains (lactobacillus acidophilus, lactobacillus casei, lactobacillus helveticus, lactobacillus plantarum, lactobacillus rhamnosus, lactococcus lactis, streptococcus thermophilus and two paenibacillus polymyxa strains) against m. haemolytica were evaluated using a spot-on-lawn method. probiotic ... | 2017 | 28178767 |
| biosorption of silver cations onto lactococcus lactis and lactobacillus casei isolated from dairy products. | the current work deals with the phenomenon of silver cations uptake by two kinds of bacteria isolated from dairy products. the mechanism of sorption of silver cations by lactococcus lactis and lactobacillus casei bacteria was investigated. inductively coupled plasma-mass spectrometry (icp-ms) was used for determination of silver concentration sorbed by bacteria. analysis of charge distribution was conducted by diffraction light scattering method. changes in the ultrastructure of lactococcus lact ... | 2017 | 28362838 |
| heme derived from corynebacterium glutamicum: a potential iron additive for swine and an electron carrier additive for lactic acid bacterial culture. | to investigate the potential applications of bacterial heme, aminolevulinic acid synthase (hema) was expressed in a corynebacterium glutamicum ha strain that had been adaptively evolved against oxidative stress. the red pigment from the constructed strain was extracted and it exhibited the typical heme absorbance at 408 nm from the spectrum. to investigate the potential of this strain as an iron additive for swine, a prototype feed additive was manufactured in pilot scale by culturing the strain ... | 2017 | 28035120 |
| target identification of volatile metabolites to allow the differentiation of lactic acid bacteria by gas chromatography-ion mobility spectrometry. | the purpose of this work was to study the potential of gas chromatography-ion mobility spectrometry (gc-ims) to differentiate lactic acid bacteria (lab) through target identification and fingerprints of volatile metabolites. the lab selected were used as reference strains for their influence in the flavour of cheese. the four strains of lab can be distinguished by the fingerprints generated by the volatile organic compounds (vocs) emitted. 2-butanone, 2-pentanone, 2-heptanone and 3-methyl-1-buta ... | 2017 | 27855912 |
| bacterial microbiota of kazakhstan cheese revealed by single molecule real time (smrt) sequencing and its comparison with belgian, kalmykian and italian artisanal cheeses. | in kazakhstan, traditional artisanal cheeses have a long history and are widely consumed. the unique characteristics of local artisanal cheeses are almost completely preserved. however, their microbial communities have rarely been reported. the current study firstly generated the single molecule, real-time (smrt) sequencing bacterial diversity profiles of 6 traditional artisanal cheese samples of kazakhstan origin, followed by comparatively analyzed the microbiota composition between the current ... | 2017 | 28068902 |
| influence of oregano essential oil on traditional argentinean cheese elaboration: effect on lactic starter cultures. | the aim of this work is to study the oregano essential oil (oeo) composition from northwestern argentinean regions and to evaluate its effect on the lactic starter cultures. the oregano used, origanum vulgare var hirtum, was obtained from andalgalá, catamarca. the essential oil presented high amounts of α-terpinene (10%), γ-terpinene (15.1%), terpinen-4-ol (15.5%) and thymol (13.0%) as the main components. no negative effect on growth or metabolic activity of lactic acid bacteria streptococcus t ... | 2017 | 27577090 |
| short communication: salt tolerance of lactococcus lactis r-604 as influenced by mild stresses from ethanol, heat, hydrogen peroxide, and uv light. | lactococcus lactis is a culture widely used in salt-containing dairy products. salt hinders bacterial growth, but exposure to environmental stress may protect cells against subsequent stress, including salt. the objective of this study was to evaluate the salt tolerance of l. lactis r-604 after exposure to various stresses. the culture was subjected to 10% (vol/vol) ethanol for 30 min, mild heat at 52°c for 30 min, 15 mm hydrogen peroxide for 30 min, or uv light (254 nm) for 5 min and compared w ... | 2017 | 28390720 |
| analyses of the probiotic property and stress resistance-related genes of lactococcus lactis subsp. lactis ncdo 2118 through comparative genomics and in vitro assays. | lactococcus lactis subsp. lactis ncdo 2118 was recently reported to alleviate colitis symptoms via its anti-inflammatory and immunomodulatory activities, which are exerted by exported proteins that are not produced by l. lactis subsp. lactis il1403. here, we used in vitro and in silico approaches to characterize the genomic structure, the safety aspects, and the immunomodulatory activity of this strain. through comparative genomics, we identified genomic islands, phage regions, bile salt and aci ... | 2017 | 28384209 |
| african fermented dairy products - overview of predominant technologically important microorganisms focusing on african streptococcus infantarius variants and potential future applications for enhanced food safety and security. | milk is a major source of nutrients, but can also be a vehicle for zoonotic foodborne diseases, especially when raw milk is consumed. in africa, poor processing and storage conditions contribute to contamination, outgrowth and transmission of pathogens, which lead to spoilage, reduced food safety and security. fermentation helps mitigate the impact of poor handling and storage conditions by enhancing shelf life and food safety. traditionally-fermented sour milk products are culturally accepted a ... | 2017 | 28364623 |
| draft genome sequences of 24 lactococcus lactis strains. | the lactic acid bacterium lactococcus lactis is widely used for the production of fermented dairy products. here, we present the draft genome sequences of 24 l. lactis strains isolated from different environments and geographic locations. | 2017 | 28360177 |
| comparative and functional genomics of the lactococcus lactis taxon; insights into evolution and niche adaptation. | lactococcus lactis is among the most widely studied lactic acid bacterial species due to its long history of safe use and economic importance to the dairy industry, where it is exploited as a starter culture in cheese production. | 2017 | 28356072 |
| a high-throughput qpcr system for simultaneous quantitative detection of dairy lactococcus lactis and leuconostoc bacteriophages. | simultaneous quantitative detection of lactococcus (lc.) lactis and leuconostoc species bacteriophages (phages) has not been reported in dairies using undefined mixed-strain dl-starters, probably due to the lack of applicable methods. we optimized a high-throughput qpcr system that allows simultaneous quantitative detection of lc. lactis 936 (now sk1virus), p335, c2 (now c2virus) and leuconostoc phage groups. component assays are designed to have high efficiencies and nearly the same dynamic det ... | 2017 | 28339484 |
| biofortification of riboflavin and folate in idli batter, based on fermented cereal and pulse, by lactococcus lactis n8 and saccharomyces boulardii saa655 in idli batter. | lactococcus lactis n8 and saccharomyces boulardii saa655 were investigated for their ability to synthesize b- vitamins (riboflavin and folate) and their functional role as microbial starters in idli fermentation. | 2017 | 28339160 |
| dynamic equilibrium on dna defines transcriptional regulation of a multidrug binding transcriptional repressor, lmrr. | lmrr is a multidrug binding transcriptional repressor that controls the expression of a major multidrug transporter, lmrcd, in lactococcus lactis. promiscuous compound ligations reduce the affinity of lmrr for the lmrcd operator by several fold to release the transcriptional repression; however, the affinity reduction is orders of magnitude smaller than that of typical transcriptional repressors. here, we found that the transcriptional regulation of lmrr is achieved through an equilibrium betwee ... | 2017 | 28325892 |
| genome engineering of virulent lactococcal phages using crispr-cas9. | phages are biological entities found in every ecosystem. although much has been learned about them in past decades, significant knowledge gaps remain. manipulating virulent phage genomes is challenging. to date, no efficient gene-editing tools exist for engineering virulent lactococcal phages. lactococcus lactis is a bacterium extensively used as a starter culture in various milk fermentation processes, and its phage sensitivity poses a constant risk to the cheese industry. the lactococcal phage ... | 2017 | 28324650 |
| recombinant lactococcus lactis expressing haemagglutinin from a polish avian h5n1 isolate and its immunological effect in preliminary animal trials. | lactic acid bacteria (lab) are gram-positive, nonpathogenic microorganisms that are gaining much interest as antigen producers for development of live vaccine vectors. heterologous proteins of different origin have been successfully expressed in various lab species, including lactococcus lactis. recombinant l. lactis strains have been shown to induce specific local and systemic immune responses against various antigens. our study aimed at constructing a l. lactis strain expressing haemagglutinin ... | 2017 | 28321412 |
| development of lactococcus lactis encoding fluorescent proteins, gfp, mcherry and irfp regulated by the nisin-controlled gene expression system. | fluorescent proteins are useful reporter molecules for a variety of biological systems. we present an alternative strategy for cloning reporter genes that are regulated by the nisin-controlled gene expression (nice) system. lactoccocus lactis was genetically engineered to express green fluorescent protein (gfp), mcherry or near-infrared fluorescent protein (irfp). the reporter gene sequences were optimized to be expressed by l. lactis using inducible promoter pnis within the pnz8048 vector. expr ... | 2017 | 28318334 |
| lactococcus lactis carrying a dna vaccine coding for the esat-6 antigen increases il-17 cytokine secretion and boosts the bcg vaccine immune response. | a regimen utilizing bcg and another vaccine system as a booster may represent a promising strategy for the development of an efficient tuberculosis vaccine for adults. in a previous work, we confirmed the ability of lactococcus lactis fnbpa+ (pvalac:esat-6), a live mucosal dna vaccine, to produce a specific immune response in mice after oral immunization. in the present study, we examined the immunogenicity of this strain as a booster for the bcg vaccine in mice. | 2017 | 28314076 |
| effect of co-overexpression of nisin key genes on nisin production improvement in lactococcus lactis ls01. | nisin is a small antimicrobial peptide produced by several subset strains of lactococcus lactis. to improve nisin yield in the producer l. lactis ls01, we proposed a successive fusion of nisa with nisrk and nisfeg into a single shuttle expression vector pmg36e under the control of the native strong constitutive promoter p32. subsequently, the recombinant vectors were transplanted into the producer cell through electroporation. nisin productivity was determined through sodium dodecyl sulfate-poly ... | 2017 | 28303477 |
| draft genome sequences of 11 lactococcus lactis subsp. cremoris strains. | the lactic acid bacterium lactococcus lactis is widely used for the fermentation of dairy products. here, we present the draft genome sequences of 11 l. lactis subsp. cremoris strains isolated from different environments. | 2017 | 28302789 |
| phage biodiversity in artisanal cheese wheys reflects the complexity of the fermentation process. | dairy fermentations constitute a perfect "breeding ground" for bacteriophages infecting starter cultures, particularly strains of lactococcus lactis. in modern fermentations, these phages typically belong to one of three groups, i.e., the 936, p335, and c2 phage groups. traditional production methods present fewer chemical and physical barriers to phage proliferation compared to modern production systems, while the starter cultures used are typically complex, variable, and undefined. in the curr ... | 2017 | 28300778 |
| semi-microbiological synthesis of an active lysinoalanine-bridged analog of glucagon-like-peptide-1. | some modified glucagon-like-peptide-1 (glp-1) analogs are highly important for treating type 2 diabetes. here we investigated whether glp-1 analogs expressed in lactococcus lactis could be substrates for modification and export by the nisin dehydratase and transporter enzyme. subsequently we introduced a lysinoalanine by coupling a formed dehydroalanine with a lysine and investigated the structure and activity of the formed lysinoalanine-bridged glp-1 analog. our data show: (i) glp-1 fused to th ... | 2017 | 28300673 |
| uvra expression of lactococcus lactis nz9000 improve multiple stresses tolerance and fermentation of lactic acid against salt stress. | lactococcus lactis is subjected to several stressful conditions during industrial fermentation including oxidation, heating and cooling, acid, high osmolarity/dehydration and starvation. dna lesion is a major cause of genetic instability in l. lactis that usually occurs at a low frequency, but it is greatly enhanced by environmental stresses. dna damages produced by these environmental stresses are thought to induce dna double-strand breaks, leading to illegitimate recombination. nucleotide exci ... | 2017 | 28298677 |
| lactococcus lactis kr-050l inhibit il-6/stat3 activation. | the purpose of this study was to investigate il-6/stat3 inhibitory activity using lactic acid bacteria (labs) isolated from gajuknamu kimchi. | 2017 | 28294465 |
| dual recombinant lactococcus lactis for enhanced delivery of dna vaccine reporter plasmid pperdby. | food grade lactococcus lactis (l. lactis) has been widely used as an antigen and dna delivery vehicle. we had previously reported the use of non-invasive l. lactis for the delivery of newly constructed immunostimulatory dna vaccine reporter plasmid, pperdby. in the present report, we outline the construction of dual recombinant l. lactis expressing internalin a of listeria monocytogenes and harbouring pperdby (ll inla+ pperdby) to enhance the dna delivery efficiency of l. lactis. after confirmat ... | 2017 | 28258689 |
| genomic characterization of dairy associated leuconostoc species and diversity of leuconostocs in undefined mixed mesophilic starter cultures. | undefined mesophilic mixed (dl-type) starter cultures are composed of predominantly lactococcus lactis subspecies and 1-10% leuconostoc spp. the composition of the leuconostoc population in the starter culture ultimately affects the characteristics and the quality of the final product. the scientific basis for the taxonomy of dairy relevant leuconostocs can be traced back 50 years, and no documentation on the genomic diversity of leuconostocs in starter cultures exists. we present data on the le ... | 2017 | 28217118 |
| antimicrobial treatments to control listeria monocytogenes in queso fresco. | queso fresco, is a hispanic non-fermented cheese highly susceptible to contamination with l. monocytogenes. this research was aimed to determine the effect of gras antimicrobial ingredients to control l. monocytogenes. antimicrobials included caprylic acid (ca), nisaplin(®) (n, 2.5% nisin), a mixture of sodium lactate and sodium diacetate (sl/sd), lactococcus lactis sbp. lactis dpc 3147, monolaurin, and lactic acid (la). batches of queso fresco curds were inoculated with 10(4) cfu/g and stored a ... | 2017 | 28213034 |
| biocidal inactivation of lactococcus lactis bacteriophages: efficacy and targets of commonly used sanitizers. | lactococcus lactis strains, being intensely used in the dairy industry, are particularly vulnerable to members of the so-called 936 group of phages. sanitization and disinfection using purpose-made biocidal solutions is a critical step in controlling phage contamination in such dairy processing plants. the susceptibility of 36 936 group phages to biocidal treatments was examined using 14 biocides and commercially available sanitizers. the targets of a number of these biocides were investigated b ... | 2017 | 28210242 |
| hsp65-producing lactococcus lactis prevents inflammatory intestinal disease in mice by il-10- and tlr2-dependent pathways. | heat shock proteins (hsps) are highly expressed at all sites of inflammation. as they are ubiquitous and immunodominant antigens, these molecules represent good candidates for the therapeutic use of oral tolerance in autoimmune and chronic inflammatory diseases. evidences from human and animal studies indicate that inflammatory bowel disease (ibd) results from uncontrolled inflammatory responses to intestinal microbiota. hsps are immunodominant proteins expressed by several immune cells and by c ... | 2017 | 28194152 |
| secretion of biologically active pancreatitis-associated protein i (pap) by genetically modified dairy lactococcus lactis nz9000 in the prevention of intestinal mucositis. | mucositis is one of the most relevant gastrointestinal inflammatory conditions in humans, generated by the use of chemotherapy drugs, such as 5-fluoracil (5-fu). 5-fu-induced mucositis affects 80% of patients undergoing oncological treatment causing mucosal gut dysfunctions and great discomfort. as current therapy drugs presents limitations in alleviating mucositis symptoms, alternative strategies are being pursued. recent studies have shown that the antimicrobial pancreatitis-associated protein ... | 2017 | 28193209 |
| identification of key residues in the nisk sensor region for nisin biosynthesis regulation. | histidine kinase (hk) nisk is well known to sense lantibiotic nisin for regulating the biosynthesis of nisin. nisk possesses two trans-membrane segments and a large extracellular region and nisin contains 34 amino acids with five lanthionine rings. unlike most peptide sensing hk with multi trans-membrane segments, nisk is a representative of a group of rarely reported hk that sense peptide as ligand. to reveal how nisk senses nisin molecule to regulate nisin biosynthesis, we constructed a report ... | 2017 | 28184221 |
| genetic and functional characterisation of the lactococcal p335 phage-host interactions. | despite continuous research efforts, bacterio(phages) infecting lactococcus lactis starter strains persist as a major threat to dairy fermentations. the lactococcal p335 phages, which are currently classified into four sub-groups (i-iv), are the second most frequently isolated phage group in an industrial dairy context. | 2017 | 28183268 |
| clumping factor a, von willebrand factor-binding protein and von willebrand factor anchor staphylococcus aureus to the vessel wall. | essentials staphylococcus aureus (s. aureus) binds to endothelium via von willebrand factor (vwf). secreted vwf-binding protein (vwbp) mediates s. aureus adhesion to vwf under shear stress. vwbp interacts with vwf and the sortase a-dependent surface protein clumping factor a (clfa). vwf-vwbp-clfa anchor s. aureus to vascular endothelium under shear stress. | 2017 | 28182324 |
| applications of genetically modified immunobiotics with high immunoregulatory capacity for treatment of inflammatory bowel diseases. | inflammatory bowel diseases (ibds), including ulcerative colitis and crohn's disease, are chronic inflammatory diseases characterized by dysregulated immune responses of the gastrointestinal tract. in recent years, the incidence of ibds has increased in developed nations, but their prophylaxis/treatment is not yet established. site-directed delivery of molecules showing anti-inflammatory properties using genetically modified (gm)-probiotics shows promise as a new strategy for the prevention and ... | 2017 | 28179904 |
| crystallographic structure of recombinant lactococcus lactis prolidase to support proposed structure-function relationships. | lactococcus lactis prolidase (xaa-pro dipeptidase; ec.3.4.13.9) is unique from other prolidases by showing allosteric behaviour, substrate inhibition, and metal-dependent substrate specificity. we have previously shown several critical residues for these characteristics using site-directed mutagenesis and amino acid sequence-based models. in this present study, the three-dimensional structure of recombinant l. lactis prolidase was determined by x-ray crystallography at 2.25å resolution in order ... | 2017 | 28179139 |
| butanol is cytotoxic to lactococcus lactis while ethanol and hexanol are cytostatic. | lactic acid bacteria currently used extensively by the dairy industry have a superior tolerance towards small chain alcohols, which makes them interesting targets for use in future bio-refineries. the mechanism underlying the alcohol tolerance of lactic acid bacteria has so far received little attention. in the present study the physiological alcohol stress response of lactococcus lactis subsp. cremoris mg1363 towards the primary, even-chain alcohols; ethanol, butanol, and hexanol was characteri ... | 2017 | 28141494 |
| secretory expression and surface display of a new and biologically active single-chain insulin (sci-59) analog by lactic acid bacteria. | insulin plays an important role in drug therapies for diabetes mellitus and as the main route of insulin delivery, subcutaneous injection may cause local discomfort, hypoglycemia, hyperinsulinemia, and patient non-compliance. therefore, oral delivery of insulin is more preferred. however, there is a low bioavailability due to insulin degradation by proteolytic enzymes and severe ph conditions along the gastrointestinal tract. in order to use the food-grade bacteria lactic acid bacteria (lab) as ... | 2017 | 28120013 |
| reversal of diabetes in nod mice by clinical-grade proinsulin and il-10-secreting lactococcus lactis in combination with low-dose anti-cd3 depends on the induction of foxp3-positive t cells. | the introduction of β-cell autoantigens via the gut through lactococcus lactis (l. lactis) has been demonstrated to be a promising approach for diabetes reversal in nod mice. here we show that a combination therapy of low-dose anti-cd3 with a clinical-grade self-containing l. lactis, appropriate for human application, secreting human proinsulin and interleukin-10, cured 66% of mice with new-onset diabetes, which is comparable to therapy results with plasmid-driven l. lactis initial blood glucose ... | 2017 | 28108611 |
| regulation of pi-2b pilus expression in hypervirulent streptococcus agalactiae st-17 bm110. | the widely spread streptococcus agalactiae (also known as group b streptococcus, gbs) "hypervirulent" st17 clone is strongly associated with neonatal meningitis. the pi-2b locus is mainly found in st17 strains but is also present in a few non st17 human isolates such as the st-7 prototype strain a909. here, we analysed the expression of the pi-2b pilus in the st17 strain bm110 as compared to the non st17 a909. comparative genome analyses revealed the presence of a 43-base pair (bp) hairpin-like ... | 2017 | 28107386 |
| changes in biosynthesis of exopolysaccharide in lactococcus lactis subspecies cremoris treated by moderate pulsed electric field treatment. | metabolome analysis and physicochemical analyses were executed with cell extracts of a lactococcus lactis subspecies cremoris strain treated by moderate pulsed electric field (pef) to elucidate the mechanism of enhanced production of exopolysaccharide (eps) by the treatment. metabolome analysis by capillary electrophoresis time of flight mass spectrometry annotated 224 metabolites from the cytoplasmic extract of the strain, which, however, showed no significant changes in metabolites related to ... | 2017 | 28093044 |
| bioethanol production from cellulosic hydrolysates by engineered industrial saccharomyces cerevisiae. | even though industrial yeast strains exhibit numerous advantageous traits for the production of bioethanol, their genetic manipulation has been limited. this study demonstrates that an industrial polyploidy saccharomyces cerevisiae jhs200 can be engineered through cas9 (crispr associated protein 9)-based genome editing. specifically, we generated auxotrophic mutants and introduced a xylose metabolic pathway into the auxotrophic mutants. as expected, the engineered strain (jx123) enhanced ethanol ... | 2017 | 28088640 |
| enhanced xylose fermentation by engineered yeast expressing nadh oxidase through high cell density inoculums. | accumulation of reduced byproducts such as glycerol and xylitol during xylose fermentation by engineered saccharomyces cerevisiae hampers the economic production of biofuels and chemicals from cellulosic hydrolysates. in particular, engineered s. cerevisiae expressing nadph-linked xylose reductase (xr) and nad(+)-linked xylitol dehydrogenase (xdh) produces substantial amounts of the reduced byproducts under anaerobic conditions due to the cofactor difference of xr and xdh. while the additional e ... | 2017 | 28070721 |
| in vitro assessment of the probiotic potential of lactococcus lactis lmg 7930 against ruminant mastitis-causing pathogens. | mastitis in dairy ruminants is considered to be the most expensive disease to farmers worldwide. recently, the intramammary infusion of lactic acid bacteria has emerged as a potential new alternative to antibiotics for preventing and treating bovine mastitis. in this study we have investigated in vitro the probiotic potential of lactococcus lactis lmg 7930, a food-grade and nisin-producing strain, against mastitis-causing pathogens. we have characterized its carbohydrate fermentation and antibio ... | 2017 | 28068371 |
| inhibition of staphylococcus aureus in vitro by bacteriocinogenic lactococcus lactis kth0-1s isolated from thai fermented shrimp (kung-som) and safety evaluation. | lactococcus lactis kth0-1s isolated from thai traditional fermented shrimp (kung-som) is able to produce heat-stable bacteriocin and inhibits food spoilage bacteria and food-borne pathogens. the inhibitory effect of bacteriocin remained intact after treatment with different phs and after heating, but was sensitive to some proteolytic enzymes. addition of bacteriocin kth0-1s to staphylococcus aureus cultures decreased viable cell counts by 2.8 log cfu/ml, demonstrating a bactericidal mode of acti ... | 2017 | 28058488 |
| engineering a thermostable keto acid decarboxylase using directed evolution and computationally directed protein design. | keto acid decarboxylase (kdc) is a key enzyme in producing keto acid derived higher alcohols, like isobutanol. the most active kdc's are found in mesophiles; the only reported kdc activity in thermophiles is 2 orders of magnitude less active. therefore, the thermostability of mesophilic kdc limits isobutanol production temperature. here, we report development of a thermostable 2-ketoisovalerate decarboxylase (kivd) with 10.5-fold increased residual activity after 1h preincubation at 60 °c. start ... | 2017 | 28052191 |
| dynamics of bacterial communities during manufacture and ripening of traditional caciocavallo of castelfranco cheese in relation to cows' feeding. | traditional caciocavallo of castelfranco is a semi-hard "pasta-filata" cheese produced from raw cows' milk in campania region. the aim of the present research is mainly focused on the study, by 16s rrna gene pyrosequencing and viable counts, of the dynamics of bacterial communities during manufacture and ripening of traditional caciocavallo cheese. moreover, the possible correlation between cheese microbiota and cows' feeding based on silage or hay was also evaluated. in general, except for ente ... | 2017 | 28040166 |
| dextran sulphate sodium colitis in c57bl/6j mice is alleviated by lactococcus lactis and worsened by the neutralization of tumor necrosis factor α. | tnfα has a well-established role in inflammatory bowel disease that affects the gastrointestinal tract and is usually manifested as crohn's disease or ulcerative colitis. we have compared lactococcus lactis nz9000 displaying tnfα-binding affibody with control lactococcus lactis and with anti-tnfα antibody infliximab for the treatment of mice with dextran sulphate sodium (dss)-induced colitis. l. lactis nz9000 alleviated the colitis severity one week after colitis induction with dss, more effecti ... | 2017 | 28039805 |
| novel variants of streptococcus thermophilus bacteriophages are indicative of genetic recombination among phages from different bacterial species. | bacteriophages are the main cause of fermentation failures in dairy plants. the majority of streptococcus thermophilus phages can be divided into either cos- or pac-type phages and are additionally characterized by examining the v2 region of their antireceptors. we screened a large number of s. thermophilus phages from the chr. hansen a/s collection, using pcr specific for the cos- or pac-type phages, as well as for the v2 antireceptor region. three phages did not produce positive results with t ... | 2017 | 28039135 |
| simultaneous lactic acidification and coagulation by using recombinant lactococcus lactis strain. | this study was an attempt to create a novel milk clotting procedure using a recombinant bacterium capable of milk coagulation. | 2017 | 28028882 |
| engineering robust and tunable spatial structures with synthetic gene circuits. | controllable spatial patterning is a major goal for the engineering of biological systems. recently, synthetic gene circuits have become promising tools to achieve the goal; however, they need to possess both functional robustness and tunability in order to facilitate future applications. here we show that, by harnessing the dual signaling and antibiotic features of nisin, simple synthetic circuits can direct lactococcus lactis populations to form programmed spatial band-pass structures that do ... | 2017 | 27899571 |
| whey powders are a rich source and excellent storage matrix for dairy bacteriophages. | thirteen whey powders and 5 whey powder formulations were screened for the presence of dairy bacteriophages using a representative set of 8 acid-producing lactococcus lactis and 5 streptococcus thermophilus, and 8 flavour-producing leuconostoc pseudomesenteroides and leuconostoc mesenteroides strains. lytic l. lactis phages were detected in all samples, while s. thermophilus and leuconostoc phages were present in 50% or 40% of the samples, respectively. maximal phage titers were 6×10(7) plaque-f ... | 2017 | 27835774 |
| mutagenesis of nisin's leader peptide proline strongly modulates export of precursor nisin. | the lantibiotic nisin is produced by lactococcus lactis as a precursor peptide comprising a 23 amino acid leader peptide and a 34 amino acid post-translationally modifiable core peptide. we previously demonstrated that the conserved fnld part of the leader is essential for intracellular enzyme-catalyzed introduction of lanthionines in the core peptide and also for transporter-mediated export, whereas other positions are subject to large mutational freedom. we here demonstrate that, in the absenc ... | 2017 | 27830473 |
| crystal structure and biochemical investigations reveal novel mode of substrate selectivity and illuminate substrate inhibition and allostericity in a subfamily of xaa-pro dipeptidases. | xaa-pro dipeptidase (xpd) catalyzes hydrolysis of iminopeptide bond in dipeptides containing trans-proline as a second residue. xpds are found in all living organisms and are believed to play an essential role in proline metabolism. here, we report crystal structures and extensive enzymatic studies of xpd from xanthomonas campestris (xpdxc), the first such comprehensive study of a bacterial xpd. we also report enzymatic activities of its ortholog from mycobacterium tuberculosis (xpdmt). these en ... | 2017 | 27816563 |
| convergent evolution of the arginine deiminase pathway: the arcd and arce arginine/ornithine exchangers. | the arginine deiminase (adi) pathway converts l-arginine into l-ornithine and yields 1 mol of atp per mol of l-arginine consumed. the l-arginine/l-ornithine exchanger in the pathway takes up l-arginine and excretes l-ornithine from the cytoplasm. analysis of the genomes of 1281 bacterial species revealed the presence of 124 arc gene clusters encoding the pathway. about half of the clusters contained the gene encoding the well-studied l-arginine/l-ornithine exchanger arcd, while the other half co ... | 2017 | 27804281 |
| serine-aspartate repeat protein d increases staphylococcus aureus virulence and survival in blood. | staphylococcus aureus expresses a panel of cell wall-anchored adhesins, including proteins belonging to the microbial surface components recognizing adhesive matrix molecule (mscramm) family, exemplified by the serine-aspartate repeat protein d (sdrd), which serve key roles in colonization and infection. deletion of sdrd from s. aureus subsp. aureus strain nctc8325-4 attenuated bacterial survival in human whole blood ex vivo, which was associated with increased killing by human neutrophils. rema ... | 2017 | 27795358 |
| expression of lycopene biosynthesis genes fused in line with shine-dalgarno sequences improves the stress-tolerance of lactococcus lactis. | lycopene biosynthetic genes from deinococcus radiodurans were co-expressed in lactococcus lactis to produce lycopene and improve its tolerance to stress. | 2017 | 27677495 |
| expression of prophage-encoded endolysins contributes to autolysis of lactococcus lactis. | analysis of autolysis of derivatives of lactococcus lactis subsp. cremoris mg1363 and subsp. lactis il1403, both lacking the major autolysin acma, showed that l. lactis il1403 still lysed during growth while l. lactis mg1363 did not. zymographic analysis revealed that a peptidoglycan hydrolase activity of around 30 kda is present in cell extracts of l. lactis il1403 that could not be detected in strain mg1363. a comparison of all genes encoding putative peptidoglycan hydrolases of il1403 and mg1 ... | 2017 | 27660179 |
| endosomal recognition of lactococcus lactis g121 and its rna by dendritic cells is key to its allergy-protective effects. | bacterial cowshed isolates are allergy protective in mice; however, the underlying mechanisms are largely unknown. we examined the ability of lactococcus lactis g121 to prevent allergic inflammatory reactions. | 2017 | 27544739 |
| cathelicidin-encoding lactococcus lactis promotes mucosal repair in murine experimental colitis. | the preventive effect of intrarectal administration of mouse cathelicidin (mcramp) and oral administration of mcramp-encoding lactococcus lactis (n4i) has been shown in murine experimental colitis. it is pivotal to understand the ability of n4i whether it can promote mucosal repair in existing colitis. | 2017 | 27470075 |
| heterologous expression of streptococcus mutans cnm in lactococcus lactis promotes intracellular invasion, adhesion to human cardiac tissues and virulence. | in s. mutans, the expression of the surface glycoprotein cnm mediates binding to extracellular matrix proteins, endothelial cell invasion and virulence in the galleria mellonella invertebrate model. to further characterize cnm as a virulence factor, the cnm gene from s. mutans strain omz175 was expressed in the non-pathogenic lactococcus lactis nz9800 using a nisin-inducible system. despite the absence of the machinery necessary for cnm glycosylation, western blot and immunofluorescence microsco ... | 2017 | 27260618 |
| getting to the heart of the matter: role of streptococcus mutans adhesin cnm in systemic disease. | 2017 | 27410461 | |
| the group a streptococcus serotype m2 pilus plays a role in host cell adhesion and immune evasion. | group a streptococcus (gas), or streptococcus pyogenes, is a human pathogen that causes diseases ranging from skin and soft tissue infections to severe invasive diseases, such as toxic shock syndrome. each gas strain carries a particular pilus type encoded in the variable fibronectin-binding, collagen-binding, t antigen (fct) genomic region. here, we describe the functional analysis of the serotype m2 pilus encoded in the fct-6 region. we found that, in contrast to other investigated gas pili, t ... | 2017 | 27741558 |
| secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by lactococcus lactis. | interleukin 6 (il-6) is an important pathogenic factor in development of various inflammatory and autoimmune diseases and cancer. blocking antibodies against molecules associated with il-6/il-6 receptor signaling are an attractive candidate for the prevention or therapy of these diseases. in this study, we developed a genetically modified strain of lactococcus lactis secreting a single-chain variable fragment antibody against mouse il-6 (il6scfv). an il6scfv-secretion vector was constructed by c ... | 2017 | 27722916 |
| production of the small heat shock protein lo18 from oenococcus oeni in lactococcus lactis improves its stress tolerance. | lactococcus lactis is a lactic acid bacterium widely used in cheese and fermented milk production. during fermentation, l. lactis is subjected to acid stress that impairs its growth. the small heat shock protein (shsp) lo18 from the acidophilic species oenococcus oeni was expressed in l. lactis. this shsp is known to play an important role in protein protection and membrane stabilization in o. oeni. the role of this shsp could be studied in l. lactis, since no gene encoding for shsp has been det ... | 2017 | 27318622 |
| engineering bacteria to manufacture functionalized polyester beads. | the ability to generate tailor-made, functionalized polyester (polyhydroxyalkanoate, pha) beads in bacteria by harnessing their natural carbon-storage granule production system is an exciting recent development. proteins that naturally attach to the polyester granule core were rationally engineered to enable in vivo production of pha beads which are applicable in bioseparation, protein purification, enzyme immobilization and diagnostics and which show advantageous properties toward the developme ... | 2017 | 22705844 |
| identification and analysis of a novel group of bacteriophages infecting the lactic acid bacterium streptococcus thermophilus. | we present the complete genome sequences of four members of a novel group of phages infecting streptococcus thermophilus, designated here as the 987 group. members of this phage group appear to have resulted from genetic exchange events, as evidenced by their "hybrid" genomic architecture, exhibiting dna sequence relatedness to the morphogenesis modules of certain p335 group lactococcus lactis phages and to the replication modules of s. thermophilus phages. all four identified members of the 987 ... | 2016 | 27316953 |
| enhance nisin yield via improving acid-tolerant capability of lactococcus lactis f44. | traditionally, nisin was produced industrially by using lactococcus lactis in the neutral fermentation process. however, nisin showed higher activity in the acidic environment. how to balance the ph value for bacterial normal growth and nisin activity might be the key problem. in this study, 17 acid-tolerant genes and 6 lactic acid synthetic genes were introduced in l. lactis f44, respectively. comparing to the 2810 iu/ml nisin yield of the original strain f44, the nisin titer of the engineered ... | 2016 | 27306587 |
| pellet feed adsorbed with the recombinant lactococcus lactis bfe920 expressing sima antigen induced strong recall vaccine effects against streptococcus iniae infection in olive flounder (paralichthys olivaceus). | the aim of this study was to develop a fish feed vaccine that provides effective disease prevention and convenient application. a lactic acid bacterium (lab), lactococcus lactis bfe920, was modified to express the sima antigen, a membrane protein of streptococcus iniae. the antigen was engineered to be expressed under the nisin promoter, which is induced by nisin produced naturally by the host lab. various sizes (40 ± 3.5 g, 80 ± 2.1 g, and 221 ± 2.4 g) of olive flounder (paralichthys olivaceus) ... | 2016 | 27302864 |
| recombinant lactococcus lactis nz9000 secretes a bioactive kisspeptin that inhibits proliferation and migration of human colon carcinoma ht-29 cells. | proteinaceous bioactive substances and pharmaceuticals are most conveniently administered orally. however, the facing problems are the side effects of proteolytic degradation and denaturation in the gastrointestinal tract. in recent years, lactic acid bacteria (lab) have been verified to be a promising delivery vector for susceptible functional proteins and drugs. kiss-1 peptide, a cancer suppressor, plays a critical role in inhibiting cancer metastasis and its activity has been confirmed by dir ... | 2016 | 27287327 |