Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| purification and characterization of a ca2+-dependent novel lectin from nymphaea nouchali tuber with antiproliferative activities. | a lectin (termed nntl) was purified from the extracts of nymphaea nouchali tuber followed by anion-exchange chromatography on deae-cellulose, hydrophobic chromatography on hitrap phenyl hp and by repeated anion-exchange chromatography on hitrap q ff column. the molecular mass of the purified lectin was 27.0 ± 1.0 kda, as estimated by sds/page both in the presence and in the absence of 2-mercaptoethanol. nntl was an o-nitrophenyl ß-d-galactopyranoside sugar-specific lectin that agglutinated rat, ... | 2011 | 21291421 |
| increased dietary zinc oxide changes the bacterial core and enterobacterial composition in the ileum of piglets. | this study was conducted to investigate the effects of increased dietary zno on the bacterial core and enterobacterial composition in the small intestine of piglets that were fed diets containing a total of 124 or 3,042 mg of zn per kilogram of diet, respectively. zinc was supplemented to the basal diet as zno. bacterial 16s rrna genes of ileal dna extracts were pcr-amplified with 2 bar-coded primer sets and sequenced by 454 pyrosequencing. the bacterial core species were calculated from the rel ... | 2011 | 21383037 |
| synthesis and characterization of new types of halogenated and alkylated imidazolidineiminothiones and a comparative study of their antitumor, antibacterial, and antifungal activities. | a series of twenty novel imidazolidineiminothiones (4-8) with various substituents at n-(1) and n-(3) were synthesized by various permutations of halogenated and alkylated n-arylcyanothioformanilides (1) with aromatic isocyanates (2). preliminary screening of all compounds against ehrlich ascites carcinoma cells (eac) indicated that 5d, and 8a-c were the most active compounds as they displayed the highest percent inhibition of cell viability (80%, 70%, 80%, and 70%, respectively). thus, they wer ... | 2011 | 21440963 |
| antimicrobial activity of organotin(iv) complexes with the ligand benzil bis(benzoylhydrazone) and 4,4'-bipyridyl as coligand. | several methyltin(iv) and butyltin(iv) complexes with the ligand benzil bis(benzoylhydrazone) and 4,4'-bipyridyl as coligand were synthesised and characterized by elemental analysis and by ir, (1)h, (13)c and (119)sn nmr spectroscopies. some of them were also analyzed using single crystal x-ray diffraction. the title compounds were evaluated for their in vitro antimicrobial properties. all buthyltin complexes showed significant inhibition of gram positive bacteria, resulting bacillus subtilis, s ... | 2011 | 21443849 |
| the shape-shifting quasispecies of rna: one sequence, many functional folds. | e unus pluribum, or "of one, many", may be at the root of decoding the rna sequence-structure-function relationship. rnas embody the large majority of genes in higher eukaryotes and fold in a sequence-directed fashion into three-dimensional structures that perform functions conserved across all cellular life forms, ranging from regulating to executing gene expression. while it is the most important determinant of the rna structure, the nucleotide sequence is generally not sufficient to specify a ... | 2011 | 21603685 |
| rice dehydrin k-segments have in vitro antibacterial activity. | dehydrins are groups of plant proteins that have been shown to response to various environmental stimuli such as dehydration, elevated salinity, and low temperature. however, their roles in plant defense against microbes have not been demonstrated. in an attempt to discover plant antimicrobial proteins, we have screened a rice cdna library and isolated several cdnas coding for dehydrins. protein extracts from escherichia coli expressing these cdnas were tested for their activity against gram-pos ... | 2011 | 21639844 |
| chemical, antioxidant and antimicrobial investigations of pinus cembra l. bark and needles. | the chemical constituents and biological activity of pinus cembra l. (pinaceae), native to the central european alps and the carpathian mountains, are not well known. the aim of the present work was to examine the phenolic content, antioxidant and antimicrobial effects of hydromethanolic extracts of pinus cembra l. bark and needles. bark extract had higher concentrations of total phenolics (299.3 vs. 78.22 mg gallic acid equivalents/g extract), flavonoids (125.3 vs. 19.84 mg catechin equivalents ... | 2011 | 22143542 |
| extraction, purification and antibacterial activities of a polysaccharide from spent mushroom substrate. | to contribute towards effective exploitation and utilization of spent mushroom substrate (sms), a water-soluble polysaccharide named pl was isolated and purified from sms. the total sugar content and monosaccharide composition were analyzed by phenol-sulfuric acid method and capillary electrophoresis, and infrared spectroscopy was also performed for structure characterization. the results showed that the total sugar content of crude polysaccharide from sms was about 25.8%, the polysaccharide con ... | 2011 | 22138450 |
| microbiological analysis of the mummy of st. marcian. | the paper provides the results of the microbiological analysis of samples taken from the mummified remains of st. marcian. microorganisms found belong to bacteria and fungi of the genera aspergillus, bacillus, penicillium, sarcina, and shewanella which are all apart of the common air, soil, and human microbial flora and therefore present no health hazard. | 2011 | 22053579 |
| [role of the snora gene in nogalamycin biosynthesis by strain streptomyces nogalater lv65 ]. | 2011 | 22073550 | |
| sarcina organisms in the gastrointestinal tract: a clinicopathologic and molecular study. | sarcina organisms were first observed in and recorded from the stomach contents of a patient suffering from vomiting by john goodsir in 1842. since that time, their fine structure, phylogenetic classification, and biochemical characteristics have been described. although numerous cases of fatal disease have been attributed to this organism in the veterinary literature, only a few human cases have been documented. as a result, whether this organism causes disease in humans has not been definitive ... | 2011 | 21997690 |
| in vitro antibacterial, cytotoxic and antioxidant activities of plant nephelium longan. | the petroleum ether, chloroform and ethyl acetate fractions of ethanol extract of leaf and stem from the plant nephelium longan (fam-sapindaceae) was subjected to antioxidant, antibacterial and cytotoxic activity. all the fractions showed potent antioxidant activity, of which the ethyl acetate and chloroform fraction of leaf demonstrated the strongest antioxidant activity with the ic50 value of 44.28 and 44.31 microg ml(-1), respectively. the petroleum ether extracts (500 microg disc(-1)) of lea ... | 2010 | 20415149 |
| emphysematous gastritis caused by sarcina ventriculi. | 2010 | 20538273 | |
| translation of henrich klebahn's 'damaging agents of the klippfish - a contribution to the knowledge of the salt-loving organisms'. | abstract: | 2010 | 20569477 |
| modeling of pyruvate decarboxylases from ethanol producing bacteria. | pyruvate decarboxylase (pdc) is a key enzyme in homoethanol fermentation process, which decarboxylates 2-keto acid pyruvate into acetaldehyde and carbon dioxide. pdc enzymes from potential ethanol-producing bacteria such as zymomonas mobilis, zymobacter palmae and sarcina ventriculi have different k(m) and k(cat) values for the substrate pyruvate at their respective optimum ph. in this study, the putative three-dimensional structures of pdc dimer of z. palmae pdc and s. ventriculi pdc were gener ... | 2010 | 20975902 |
| fervidicella metallireducens gen. nov., sp. nov., a thermophilic, anaerobic bacterium from geothermal waters. | a strictly anaerobic, thermophilic bacterium, designated strain aeb(t), was isolated from microbial mats colonizing a run-off channel formed by free-flowing thermal water from a bore well (registered number 17263) of the great artesian basin, australia. cells of strain aeb(t) were slightly curved rods (2.5-6.0x1.0 mum) that stained gram-negative and formed spherical terminal to subterminal spores. the strain grew optimally in tryptone-yeast extract-casamino acids medium at 50 degrees c (range 37 ... | 2010 | 19671710 |
| first solid state alkaline-earth complexes of monensic acid a (monh): crystal structure of [m(mon)2(h (2)o)2] (m = mg, ca), spectral properties and cytotoxicity against aerobic gram-positive bacteria. | alkaline-earth metal complexes of the monoanionic form of the polyether ionophore monensin a were isolated for the first time in solid state and were structurally characterized using various spectroscopic methods (ir, nmr, fab-ms). the stoichiometric reaction of monensic acid (monh) with m(2+) (m = mg, ca) in the presence of an organic base leads to the formation of mononuclear complexes of composition [m(mon)(2)(h(2)o)(2)]. the structures of magnesium (1) and calcium (2) monensin complexes in t ... | 2010 | 19768636 |
| hptlc densitometric evaluation of tissue culture extracts of nothapodytes foetida compared to conventional extracts for camptothecin content and antimicrobial activity. | tissue culture technique is becoming popular because of its well-known ability to enhance the content of secondary metabolites in plants. callus tissue cultures of nothapodytes foetida were developed using 250 different medium compositions to optimize this procedure. methanolic extracts of callus (mec) and of various parts of n. foetida were comparatively analyzed for camptothecin content, and a high performance thin layer chromatography method was developed for its quantitation. chloroform-ethy ... | 2010 | 19862669 |
| genes involved in long-chain alkene biosynthesis in micrococcus luteus. | aliphatic hydrocarbons are highly appealing targets for advanced cellulosic biofuels, as they are already predominant components of petroleum-based gasoline and diesel fuels. we have studied alkene biosynthesis in micrococcus luteus atcc 4698, a close relative of sarcina lutea (now kocuria rhizophila), which 4 decades ago was reported to biosynthesize iso- and anteiso-branched, long-chain alkenes. the underlying biochemistry and genetics of alkene biosynthesis were not elucidated in those studie ... | 2010 | 20038703 |
| photodynamic antimicrobial chemotherapy by liposome-encapsulated water-soluble photosensitizers. | photodynamic antimicrobial chemotherapy is an alternative method for killing bacterial cells in view of the increasing problem of multi-antibiotic resistance. we examined the effect of three water-soluble photosensitizers (phs): methylene blue (mb), neutral red (nr) and rose bengal (rb) on gram-positive and gram-negative bacteria. we compared the efficacy of phs in their free form and encapsulated in liposomal formulations against various bacterial strains, and determined conditions for the effe ... | 2010 | 20644595 |
| bar-coded pyrosequencing of 16s rrna gene amplicons reveals changes in ileal porcine bacterial communities due to high dietary zinc intake. | feeding high levels of zinc oxide to piglets significantly increased the relative abundance of ileal weissella spp., leuconostoc spp., and streptococcus spp., reduced the occurrence of sarcina spp. and neisseria spp., and led to numerical increases of all gram-negative facultative anaerobic genera. high dietary zinc oxide intake has a major impact on the porcine ileal bacterial composition. | 2010 | 20709843 |
| examination of equine glandular stomach lesions for bacteria, including helicobacter spp by fluorescence in situ hybridisation. | the equine glandular stomach is commonly affected by erosion and ulceration. the aim of this study was to assess whether bacteria, including helicobacter, could be involved in the aetiology of gastric glandular lesions seen in horses. | 2010 | 20298612 |
| clostridial abomasal disease in connecticut dairy calves. | over 2 years, 24 dairy calves died of emphysematous abomasitis and abomasal bloat. anaerobic cultures of necrotic abomasal mucosa yielded clostridium perfringens from 10 of 15 calves. sarcina were observed in 17 of 22 examined histologically. a change in the antibiotic regimen for newborns and improved sanitizing of feeding utensils eliminated further losses. | 2009 | 19881926 |
| crystal structure and properties of the copper(ii) complex of sodium monensin a. | the preparation and structural characterization of a new copper(ii) complex of the polyether ionophorous antibiotic sodium monensin a (monna) are described. sodium monensin a binds cu(ii) to produce a heterometallic complex of composition [cu(monna)(2)cl(2)].h(2)o, 1. the crystallographic data of 1 show that the complex crystallizes in monoclinic space group c2 with cu(ii) ion adopting a distorted square-planar geometry. copper(ii) coordinates two anionic sodium monensin ligands and two chloride ... | 2009 | 19748125 |
| biodegradation of international jet a-1 aviation fuel by microorganisms isolated from aircraft tank and joint hydrant storage systems. | microorganisms contaminating international jet a-1 aircraft fuel and fuel preserved in joint hydrant storage tank (jhst) were isolated, characterized and identified. the isolates were bacillus subtillis, bacillus megaterium, flavobacterium oderatum, sarcina flava, micrococcus varians, pseudomonas aeruginosa, bacillus licheniformis, bacillus cereus and bacillus brevis. others included candida tropicalis, candida albicans, saccharomyces estuari, saccharomyces cerevisiae, schizosaccharomyces pombe, ... | 2009 | 19452113 |
| extensive carbon isotopic heterogeneity among methane seep microbiota. | to assess and study the heterogeneity of delta(13)c values for seep microorganisms of the eel river basin, we studied two principally different sample sets: sediments from push cores and artificial surfaces colonized over a 14 month in situ incubation. in a single sediment core, the delta(13)c compositions of methane seep-associated microorganisms were measured and the relative activity of several metabolisms was determined using radiotracers. we observed a large range of archaeal delta(13)c val ... | 2009 | 19508338 |
| design, synthesis and antibacterial activity of novel actinonin derivatives containing benzimidazole heterocycles. | a series of novel actinonin derivatives containing a benzimidazole heterocycle linked as amide isostere have been designed and synthesized. the structures of all the synthesized compounds were confirmed by analytical and spectroscopic methods. all the compounds were evaluated in vitro against staphylococcus aureus, klebsiella pneumoniae, and sarcina lutea. among them, compound 1a with unsubstituted benzimidazole ring exhibited potent antibacterial activities. | 2009 | 18617291 |
| outbreak of subclinical mastitis in a flock of dairy goats associated with atypical staphylococcus haemolyticus. | staphylococcus haemolyticus is a pathogen frequently isolated from dairy cows and small ruminants. however, it always appears in only a few animals and not as a major pathogen. recently, in a dairy goat herd of approximately 250 milking animals, 25.6% (46/180 goats) had milk cultures with atypical highly mucoid colonies accompanied by elevated somatic cell counts. the isolates were identified as staph. haemolyticus. the present study describes the steps used in an attempt to identify the bacteri ... | 2009 | 18922198 |
| antiproliferative and antimicrobial activity of traditional kombucha and satureja montana l. kombucha. | to carry out a preliminary investigation of the biological activity of kombucha beverages from camellia sinensis l. (black tea) and satureja montana l. (winter savory tea), that have consuming acidity. | 2008 | 18979556 |
| synthesis of acyl thiourea derivatives of chitosan and their antimicrobial activities in vitro. | three different acyl thiourea derivatives of chitosan (cs) were synthesized and their structures were characterized by ft-ir spectroscopy and elemental analysis. the antimicrobial behaviors of cs and its derivatives against four species of bacteria (escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, and sarcina) and four crop-threatening pathogenic fungi (alternaria solani, fusarium oxysporum f. sp. vasinfectum, colletotrichum gloeosporioides (penz.) saec, and phyllisticta zingiber ... | 2008 | 18083151 |
| radical scavenging, antibacterial, and antiproliferative activities of melissa officinalis l. extracts. | the aromatic herb melissa officinalis l. can be used as an easily accessible source of natural antioxidants and as a possible food supplement and as a phytochemical. radical scavenging, antibacterial, and antiproliferative activities of petroleum ether, chloroform, ethyl acetate, n-butanol, and water extracts of m. officinalis l. extracts were investigated. the results of antioxidative activity, obtained by electron spin resonance spectroscopy, confirmed that investigated extracts suppressed the ... | 2008 | 18361749 |
| methanogen community in zoige wetland of tibetan plateau and phenotypic characterization of a dominant uncultured methanogen cluster zc-i. | zoige wetland of tibetan plateau is characterized by being located at a low latitude (33 degrees 56'n, 102 degrees 52'e) region and under the annual temperature around 1 degrees c. previous studies indicated that zoige wetland was one of the ch(4) emission centres in qinghai-tibetan plateau; in this study, the methanogen community in this low-latitude wetland was analysed based on the homology of 16s rrna and mcra genes retrieved from the soil. the results indicated that members of methanosarcin ... | 2008 | 18373675 |
| halosarcina pallida gen. nov., sp. nov., a halophilic archaeon from a low-salt, sulfide-rich spring. | a novel halophilic archaeon, strain bz256t, was isolated from zodletone spring, a sulfide- and sulfur-rich spring in south-western oklahoma, usa. cells were non-motile, non-flagellated cocci that divided along two axes, resulting in the formation of sarcina-like clusters. strain bz256t grew at salt concentrations ranging from 1.3 to 4.3 m nacl, with optimum growth at approximately 3.4 m, and required at least 1 mm mg2+ for growth. the ph range for growth was 5.0 to at least 8.5, and the temperat ... | 2008 | 18398182 |
| antimicrobial anthraquinones from morinda angustifolia. | investigation on morinda angustifolia resulted in the isolation of a new anthraquinone, 1,8-dihydroxy-2-methyl-3,7-dimethoxyanthraquinone (1), along with five known analogues, lucidin 3-o-beta-primeveroside (2), 1,3-dihydroxy-2-methylanthraquinone (3), lucidin- omega -ethyl ether (4), lucidin-omega-butyl ether (5) and damnacanthol (6). the new compound demonstrated significant antimicrobial activity against bacillus subtilis, escherichia coli, micrococcus luteus, sarcina lutea, candida albicans ... | 2008 | 18621113 |
| in vitro antimicrobial activity and chemical composition of the essential oil of foeniculum vulgare mill. | in our study, four samples of volatile oil from foeniculum vulgare, cultivated in different pedoclimatic conditions, were investigated for their antimicrobial activity and chemical composition. | 2008 | 20201277 |
| synthesis, structure and antimicrobial activity of manganese(ii) and cobalt(ii) complexes of the polyether ionophore antibiotic sodium monensin a. | mononuclear neutral manganese(ii) and cobalt(ii) complexes with the antibiotic sodium monensin a (mon-na, 1b) were synthesized and characterized. the crystal structures of m(mon-na)2cl2.h2o (m=mn, 2; m=co, 3) were determined by x-ray crystallography. the complexes crystallize in monoclinic space group c2 with a tetrahedrally coordinated transition metal attached to oxygen atoms of deprotonated carboxyl groups of two sodium monensin molecules and two chloride ions. the sodium ion remains in the c ... | 2008 | 17692921 |
| synthesis, spectral characterization and eukaryotic dna degradation of thiosemicarbazones and their platinum(iv) complexes. | the condensation products of acetophenone (or its derivatives), salicylaldehyde and o-hydroxy-p-methoxybenzophenone with thiosemicarbazide and ethyl- or phenyl-thiosemicarbazide are the investigated thiosemicarbazones. their reactions with h(2)ptcl(6) produced pt(iv) complexes characterized by elemental, thermal, mass, ir and electronic spectral studies. the coordination modes were found mononegative bidentate in the acetophenone derivatives and binegative tridentate in the salicylaldehyde deriv ... | 2008 | 17500028 |
| [microbial community of freshwater sponges in lake baĭkal]. | the microbial community of baikal sponges has been studied in five species belonging to the genera swartschewskia, baicalospongia, and lubomirskia of the endemic family lubomirskiidae. the results show that the total numbers of bacteria and bacterioplankton production have an effect on the growth of l. baicalensis body. bacteria of the genera pseudomonas, bacillus, micrococcus, sarcina, flavobacterium, arthrobacter, and acinetobacter living in the sponges are representatives of the baikal bacter ... | 2008 | 18771026 |
| sarcina-like bacteria associated with bloat in young lambs and calves. | sarcina species are fastidious gram-positive anaerobic bacteria that occur in cubical packets of eight or more cells. in 2006 to 2007, they were associated with cases of acute abomasal bloat in young lambs and calves. two incidents were in lambs aged three to six weeks that were found dead, with one or two cases in each of a group of 15 and 100 lambs. three incidents were recorded in small groups of calves up to 10 days of age, two cases in each incident, with the calves found dead or dying afte ... | 2008 | 18820327 |
| antimicrobial activity of the essential oil and extracts of cordia curassavica (boraginaceae). | in traditional mexican medicine cordia curassavica (jacq) roemer & schultes is used to treat gastrointestinal, respiratory and dermatological disorders in zapotitlán de las salinas, puebla (méxico). the aim of this work was to investigate antimicrobial activity of the essential oil, obtained by using clevenger distillation apparatus, and hexane, chloroform and methanol extracts from aerial parts of cordia curassavica. antimicrobial activity was evaluated against 13 bacteria and five fungal strai ... | 2007 | 17140754 |
| antibiotic prophylaxis in cerebrospinal fluid shunting: reassessment of cefotiam penetration into human csf. | shunt infection is a major complication of shunt implantation. numerous clinical studies give evidence that antibiotic prophylaxis is efficacious in preventing infections after cerebrospinal fluid shunting. in csf shunting, antibiotics need to reach sufficient concentrations not only in the blood shielding the operative field but also in tissues and the csf compartment. cefotiam is widely used for prophylaxis in neurosurgery. some clinical trials report that this beta-lactam is able to penetrate ... | 2007 | 17487803 |
| tympany, acidosis, and mural emphysema of the stomach in calves: report of cases and experimental induction. | necropsy of 4 neonatal calves with a peracute syndrome of abdominal distention, diarrhea, dehydration, shock, and death revealed abomasal tympany, marked edema, hemorrhage, and emphysema of ruminal and abomasal walls and histopathologic lesions characteristic of forestomach acidosis. the presence of acid damage and the production of substantial quantities of gas strongly suggested that the pathogenesis of the syndrome involved exuberant fermentation of intragastric substrate. this supposition le ... | 2007 | 17609349 |
| coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in lactobacillus brevis. | lactobacillus brevis atcc367 was engineered to express pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) genes in order to increase ethanol fermentation from biomass-derived residues. first, a gram-positive sarcina ventriculi pdc gene (svpdc) was introduced into l. brevis atcc 367 to obtain l. brevis bbc03. the svpdc was detected by immunoblot using an svpdc oligo peptide antiserum, but no increased ethanol was detected in l. brevis bbc03. then, an adh gene from l. brevis (bradh) was ... | 2007 | 17651394 |
| antimicrobial and general toxicity activities of gymnosperma glutinosum: a comparative study. | gymnosperma glutinosum (spreng.) less (asteraceae) is an important, and an effective herbal medicine which is wide used for the treatment of diarrhoea in mexico. we examined and compared the antibacterial and antifungal activities through the dilution method and for general toxicity activity by the brine shrimp lethality assay of two samples of gymnosperma glutinosum from two localities of mexico: san rafael-coxcatlan (puebla state) and tepeji del rio (hidalgo state). in addition, two bioactive ... | 2007 | 17110067 |
| synthesis and antimicrobial activity of some new 1,3,4-thiadiazole and 1,2,4-triazole compounds having a d,l-methionine moiety. | new 1,3,4-thiadiazole, 5a-e, and 1,2,4-triazolecompounds 6a-c, containing a d,l-methionine moiety were synthesized by intramolecular cyclization of 1,4-disubstituted thiosemicarbazides 4a-e in acid and alkaline media, respectively. the potential antimicrobial effects of the synthesized compounds were investigated using the staphylococcus aureus atcc 25923, bacillus antracis atcc 8705, bacillus cereus atcc 10987, sarcina lutea atcc 9341 and escherichia coli atcc 25922 strains. the newly synthesiz ... | 2007 | 17693957 |
| [dependence of antimicrobial effect of micelle-capsulated therapeutics on the micelle size]. | with the method of dynamic light scattering it was shown that the average size of micelles in the series of formulations based on various clindamycin salts, i. e. clindhcl+tween-20, clindbz+tween-20, clindhcl+cremafor-el and clindbz+cremafor-el increased from 6 to 20 nm. investigations with the agar diffusion method revealed that the bactericidic action of the micelle-capsulated therapeutics did not depend on the micelle size within 6 to 20 mn. the concentration of the micellar clindamycin or ge ... | 2006 | 16734357 |
| antifungal and antibacterial activities of mexican tarragon (tagetes lucida). | mexican tarragon (tagetes lucida cv. asteraceae: campanulatae) is an important, nutritious plant and an effective herbal medicine. seven coumarins, 7,8-dihydroxycoumarin (4), umbelliferone (7-hydroxycoumarin) (5), scoparone (6,7-dimethoxycoumarin) (7), esculetin (6,7-dihydroxycoumarin) (11), 6-hydroxy-7-methoxycoumarin (12), herniarin (7-methoxycoumarin) (13), and scopoletin (6-methoxy-7-hydroxycoumarin) (14), and three flavonoids, patuletin (18), quercetin (19), and quercetagetin (20), were iso ... | 2006 | 19127719 |
| metabolic engineering of a lactobacillus plantarum double ldh knockout strain for enhanced ethanol production. | lactobacillus plantarum ferments glucose through the embden-meyerhof-parnas pathway: the central metabolite pyruvate is converted into lactate via lactate dehydrogenase (ldh). by substituting ldh with pyruvate decarboxylase (pdc) activity, pyruvate may be redirected toward ethanol production instead of lactic acid fermentation. a pdc gene from the gram-positive bacterium sarcina ventriculi (spdc) was introduced into an ldh-deficient strain, l. plantarum tf103, in which both the ldhl and ldhd gen ... | 2006 | 16193282 |
| [degree of penetration of antibiotics in the parenhima of the prostate gland]. | the article presents results of bacteriological research of a secret of prostate gland and blood serum on test-culture sercina lutera atss 9341 in 57 patients with chronic bacterial prostatitis along with ampicillin sodium application through lymph and intramuscular injection of the antibiotic. | 2006 | 17312880 |
| synthesis, a case of isostructural packing, and antimicrobial activity of silver(i)quinoxaline nitrate, silver(i)(2,5-dimethylpyrazine) nitrate and two related silver aminopyridine compounds. | the synthesis and low temperature crystal structures of [ag(quinoxaline)]n(no3)n, 1, [ag(2,5-dimethylpyrazine)(no3)]n, 2 and [ag4(3-aminopyridine)4(no3)4]n 3 are presented. the quinoxaline compound forms a 1d coordination polymer with the characteristic linear 2-coordination figure of silver(i), the n-ag-n angle being 164.2(1) degrees, and only weak silver-nitrate interactions. in addition there is an interaction giving pairs of parallel chains as the main structural theme. the 2,5-dimethylpyraz ... | 2006 | 16718338 |
| variation in chemical composition and antibacterial activities of essential oils from two species of houttuynia thunb. | houttuynia thunb. (saururaceae) has been used for dozens of years in china for the treatment of cough, leucorrhea and ureteritis. the essential oils from the two species: houttuynia emeiensis and houttuynia cordata sold in china under one trade name 'yuxingcao', obtained by hydrodistillation, were analyzed by gc-ms. the results show that fifty-five components were identified and methyl nonyl ketone (2.10-40.36%), bornyl acetate (0.4-8.61%) and beta-myrcene (2.58-18.47%) were the most abundant co ... | 2006 | 16819207 |
| characterization of lactic acid bacteria and other gut bacteria in pigs by a macroarraying method. | lactic acid bacteria (lab) consist of many genera, gram-positive, and nonspore-forming micro-organisms; some members being used as probiotics while some others have negative effects on pig health. bacterial species in the gastrointestinal tract can produce antibacterial substances, reduce serum cholesterol in their host, or can be responsible for growth reduction, diarrhea, and intestinal epithelial damage. it is therefore important for the pig industry to evaluate the impact of food and farm ma ... | 2006 | 17135526 |
| construction and expression of an ethanol production operon in gram-positive bacteria. | pyruvate decarboxylase (pdc), an enzyme central to homoethanol fermentation, catalyses the non-oxidative decarboxylation of pyruvate to acetaldehyde with release of carbon dioxide. pdc enzymes from diverse organisms have different kinetic properties, thermal stability and codon usage that are likely to offer unique advantages for the development of desirable gram-positive biocatalysts for use in the ethanol industry. to examine this further, pdc genes from bacteria to yeast were expressed in the ... | 2005 | 16339947 |
| polycomplexes of poly(acrylic acid) with streptomycin sulfate and their antibacterial activity. | complex formation between streptomycin sulfate and poly(acrylic acid) has been studied in aqueous solutions by turbidimetric, potentiometric and viscometric methods as well as by ftir spectroscopy. it was shown that these polycomplexes are stabilized by electrostatic interactions. the solubility of polycomplexes was examined as a function of ph and it was found that at ph values below 3.1 the polycomplexes undergo complete dissociation or dissolution. the antimicrobial activity of the drug and i ... | 2004 | 15018981 |
| interference of isonicotinyl hydrazone in the microbiological analysis of rifampicin from anti-tuberculosis fdc products containing isoniazid. | microbiological assay is a sensitive method for the estimation of rifampicin (r). in the present study, interference due to isonicotinyl hydrazone (hyd), an interaction product of r and isoniazid (h), was checked during microbiological analysis of r, employing bacillus subtilis and sarcina lutea. the assays were done by disc diffusion method. both r and hyd showed linear log response curves in the range of 0.01-10microg. in the presence of hyd, r was overestimated when tested against s. lutea an ... | 2004 | 15522543 |
| fermentation and antimicrobial activities of extracts from different species of fungus belonging to genus, trichoderma. | the present paper discusses the effects of the crude extracts of trichoderma spp. on a number of fungal and bacterial organisms. these include paecilomyces variotii, penicillium notatum, nematospora corylii, mucor miehei, bacillus brevis, bacillus subtilis, enterobacter dissolvens and sarcina lutea. the culture broth extracts of different isolates of trichoderma harzianum, t. longibrachiatum and t. koningii cultured in different media were investigated individually for in-vitro antifungal and an ... | 2004 | 17298115 |
| synthesis and antimicrobial activity of certain novel quinoxalines. | in this study, certain 3-methyl-2-[4-(substituted amino carbonyl)anilino] quinoxalines, (2a-d) and (3a-d), were synthesized from the new key compound 2-[4-(ethoxycarbonyl)anilino]-3-methyl quinoxaline (1). in addition, a series of 2-[4-(arylidene hydrazinocarbonyl)anilino]-3-methyl quinoxalines (5a-e), as well as their cyclized oxadiazolinyl derivatives (6a-e), and a series of 2-[4-n2-acylhydrazinocarbonyl) anilino]-3-methyl quinoxalines (7a-d), as well as their cyclized oxadiazoiyl derivatives ... | 2004 | 15595408 |
| influence of nutrients on biomass evolution in an upflow anaerobic sludge blanket reactor degrading sulfate-laden organics. | this paper describes the effect of the nutrients iron (fe), nickel (ni), zinc (zn), cobalt (co), and molybdenum (mo) on biomass evolution in an upflow anaerobic sludge blanket (uasb) reactor metabolizing synthetic sulfate-laden organics at varying operating conditions during a period of 540 days. a bench-scale model of a uasb reactor was operated at a temperature of 35 degrees c for a chemical oxygen demand-to-sulfate (cod/so4(2-)) ratio of 8.59 to 2.0, a sulfate loading rate of 0.54 to 1.88 kg ... | 2004 | 16042109 |
| [methanogenic sarcina from an anaerobic microbial community degrading p-toluene sulfonate]. | the methanogenic strain mm isolated from an anaerobic microbial community degrading p-toluene sulfonate showed optimal values of temperature and ph for growth equal to 37 degrees c and 6.3-6.9, respectively. the doubling times of the isolate grown on methanol, acetate, and methylamines under the optimal conditions were 8.8, 19.1, and 10.3-28.1 h, respectively. the growth of strain mm was observed only when the cultivation medium contained casamino acids or p-toluene sulfonate. the g + c content ... | 2003 | 14526547 |
| conventional morphological and functional evaluation of the microbial populations in a sequencing batch reactor performing ebpr. | to help confirm and interpret the enhanced biological phosphorus removal (ebpr) performance of the microbial populations in a laboratory-scale activated sludge (as) system, conventional microscopic examinations were carried out. a lab-scale sequencing batch reactor (sbr), named arc, was fed with acetate, as the sole carbon source, and operated for ebpr. daily monitoring and cyclic behavior evaluation studies indicated that the system always worked for ebpr in the long run, with efficiencies depe ... | 2003 | 12929804 |
| screening of eight alkaloids and ten flavonoids isolated from four species of the genus boronia (rutaceae) for antimicrobial activities against seventeen clinical microbial strains. | eight alkaloids and ten flavonoids isolated from four species of boronia of the rutaceae were screened against 17 clinical microbial strains. of the test compounds, three acridone and one quinolone alkaloids and eight flavonoids were reported as novel natural products. screening was carried out by the standard disc diffusion method. of the tested compounds, six alkaloids and seven flavonoids including the novel products were active against six clinical strains. the active compounds showed mild t ... | 2002 | 12410551 |
| methylosarcina fibrata gen. nov., sp. nov. and methylosarcina quisquiliarum sp.nov., novel type 1 methanotrophs. | two novel species of obligate methane-oxidizing bacteria, isolated from landfill soil, were characterized. both strains were unusual in that some members of the population grew in irregularly shaped, refractile cell packets that resembled sarcina-like clusters. electron microscopy revealed that the cell packets were covered with a slime layer and the cells contained many large granular inclusion bodies. the individual cells of each strain were sometimes motile and had differing morphologies. iso ... | 2001 | 11321107 |
| production of the gram-positive sarcina ventriculi pyruvate decarboxylase in escherichia coli. | sarcina ventriculi grows in a remarkable range of mesophilic environments from ph 2 to ph 10. during growth in acidic environments, where acetate is toxic, expression of pyruvate decarboxylase (pdc) serves to direct the flow of pyruvate into ethanol during fermentation. pdc is rare in bacteria and absent in animals, although it is widely distributed in the plant kingdom. the pdc gene from s. ventriculi is the first to be cloned and characterized from a gram-positive bacterium. in escherichia col ... | 2001 | 11535783 |
| lactic acid bacteria from healthy oral cavity of thai volunteers: inhibition of oral pathogens. | the aims of the present study were to screen and characterize the antimicrobial lactic acid bacteria which were isolated from healthy oral cavities of thai volunteers, and to characterize their inhibiting substances. among 3790 isolates (suspected to be lactic acid bacteria) from 130 volunteers, five showed an appreciable effect against sarcina lutea atcc 9341, bacillus cereus atcc 11778, escherichia coli atcc 25922, staphylococcus aureus atcc 6538, streptococcus mutans dtmu 1, strep. salivarius ... | 2001 | 11168719 |
| possible involvement of sarcina ventriculi in canine and equine acute gastric dilatation. | 2000 | 11126583 | |
| sarcina -like bacteria, clostridium fallax and clostridium sordellii in lambs with abomasal bloat, haemorrhage and ulcers. | a study of abomasal disease in lambs aged 2-5 weeks, made during the period 1993-1998, included 67 cases and 45 non-affected controls. gross pathological findings included various combinations of bloat, haemorrhage and ulcers in the abomasum. sarcina -like bacteria were found in sections and smears from the abomasum of 79% (53/67) of the cases. from one case, a lamb with abomasal bloat, the anaerobic "packet"-forming sarcina ventriculi was cultivated from the abomasal contents and identified by ... | 2000 | 10684688 |
| metabolites of colletotrichum gloeosporioides, an endophytic fungus in artemisia mongolica. | a new antimicrobial metabolite, named colletotric acid (1), was isolated from a liquid culture of colletotrichum gloeosporioides, an endophytic fungus colonized inside the stem of artemisia mongolica. the structure was determined using spectroscopic methods (eims and fabms,(1)h and (13)c nmr, (1)h-(1)h cosy, hmbc, and hmqc). compound 1 inhibited the growth of bacillus subtilis, staphylococcusaureus, and sarcina lutea with minimal inhibitory concentrations (mics) of 25, 50, and 50 microg/ml, resp ... | 2000 | 11087599 |
| studies on aminopyrazoles: antibacterial activity of some novel pyrazol. | hydrazones 2a-d were prepared from diazotiazation of sulfanilamide or its derivatives followed by coupling with ethyl cyanoacetate. 3-aminopyrazoles 3a-d were obtained by refluxing of 2a-d with hydrazine hydrate in ethanol. [bis(-methylthio)methylene]malononitrile (4) was reacted with aminopyrazoles 3a-d in refluxing dmf containing triethylamine to yield the novel pyrazolo[1,5-a]pyrimidines 6a-d. the anilino derivatives 8a-h were obtained by fusion of compounds 6a-d with aromatic amines. when co ... | 2000 | 11152240 |
| attachment of the chromosome to the cell poles: the strategy for the growth of bacteria in two and three dimensions. | bacteria such as staphylococcus, lampropedia, and sarcina develop in characteristic two-or three-dimensional groups of cells. we propose here a model of how bacteria may generate such groupings by an extension of an earlier model for rod-shaped bacteria. no other mechanism for forming two- or three-dimensional structures of groups of cells has been proposed. our earlier model for division of rod-shaped bacteria into nearly equal-sized daughters assumed that the origin and terminus dna were attac ... | 1999 | 10395815 |
| absorption kinetics and bioavailability of cephalexin in the dog after oral and intramuscular administration. | the pharmacokinetics of cephalexin, a first generation cephalosporin, were investigated in dogs using two formulations marketed for humans, but also often employed by practitioners for pet therapy. cephalexin was administered to five dogs intravenously and intramuscularly as a sodium salt and by the oral route as a monohydrate. the dosage was always 20 mg/kg of active ingredient. a microbiological assay with sarcina lutea as the test organism was adopted to measure cephalexin concentrations in s ... | 1999 | 10597534 |
| antimicrobial activity and stability of tingenone derivatives. | quinone-methide triterpenes of the tingenone series were evaluated for their antimicrobial activity. these compounds were effective against bacillus cereus, b. subtilis, sarcina lutea, staphylococcus aureus, microsporum gypseum and a gram-negative bacterium, klebsiella pneumoniae. under acidic conditions, the quinone-methide part of these compounds rearranged into the divinyl-phenolic system, and the antimicrobial activity was thus lost. | 1999 | 10418334 |
| bacteriological studies of blood, tissue fluid, lymph and lymph nodes in patients with acute dermatolymphangioadenitis (dla) in course of 'filarial' lymphedema. | filarial lymphedema is complicated by frequent episodes of dermatolymphangioadenitis (dla). severe systemic symptoms during attacks of dla resemble those of septicemia. the question we asked was whether bacterial isolates can be found in the peripheral blood of patients during the episodes of dla. out of 100 patients referred to us with 'filarial' lymphedema 14 displayed acute and five subacute symptoms of dla. all were on admission blood microfilariae negative but had a positive test in the pas ... | 1999 | 10546838 |
| antimicrobial effects of aqueous plant extracts on the fungi microsporum canis and trichophyton rubrum and on three bacterial species. | aqueous extracts of 10 plants were tested for their ability to inhibit trichophyton rubrum and microsporum canis, the aetiological agents of dermal fungal infections in humans. these extracts were also evaluated for their activity against some bacteria. aqueous extracts from the leaves of inula viscosa produced detectable antifungal activity against these dermatophytes. | 1998 | 9489036 |
| the mitochondrial phosphoglyceroyl-atp-containing polymer, purinogen, is unchanged by cardiac ischaemia and reperfusion but may function in the regulation of free intracellular inorganic phosphate concentrations. | previous work in our laboratory demonstrating large unexplained systematic variations in the heart contents of free adenine nucleotides led us to propose the existence of some unrecognised sequestered form and thence to the purification of very labile acid-insoluble oligomers which we characterised as oligo[3-phospho-glyceroyl-gamma-triphospho(5')adenosine(3')] , abbreviated to (pg-atp)n. more recently, we provided evidence that these oligomers appear to be the end chains of a complex polymer lo ... | 1998 | 9652397 |
| serum and cerebrospinal fluid concentrations of rifampicin at two dose levels in children with tuberculous meningitis. | twenty patients of tuberculous meningitis (tbm) in age group of 6 months to 10 years included in the study were divided into two groups of 10 patients each. rifampicin was administered in dosage of 10 mg and 7.5 mg/kg bw to each patient of groups i and ii respectively. drug concentrations in serum and csf of these patients were measured by a microbiological tube dilution method using a strain of sarcina lutea. in group i mean serum and csf concentration was 3.84 micrograms/ml and 0.178 microgram ... | 1997 | 9465533 |
| activity of cytotoxin p4 from the venom of the cobra snake naja nigricollis on gram-positive bacteria and eukaryotic cell lines. | the cytotoxin p4 from the venom of the cobra snake naja nigricollis was examined for activity against aerobic gram-positive and gram-negative bacteria, yeasts, fungi and eucaryotic cell lines by determination of minimal inhibitory concentrations (mic) and ic50 values, respectively. cytotoxin p4 exhibits antimicrobial activity against bacillus subtilis, micrococcus flavus and sarcina lutea. targets in gram-negative bacteria and fungi apparently were not reached. toxicity against eucaryotic cells ... | 1997 | 9205785 |
| oligosaccharide beta-glucans with unusual linkages from sarcina ventriculi. | the structure of a family of unusual glucans from sarcina ventriculi has been characterized by nmr spectroscopy, methylation analysis, and mass spectrometry. one is a trisaccharide containing a beta-(1-->3) and a beta-(1-->4)-linkage. the other is a hexasaccharide that is simply a 1,4-linkage dimer of the trisaccharide unit. this is the first report of beta-glucan biosynthesis in a gram-positive organism. their occurrence in these organisms supports an even more general link between their synthe ... | 1997 | 9449765 |
| mercury and organomercurial resistance in bacteria isolated from freshwater fish of wetland fisheries around calcutta. | mercury-resistant bacteria belonging to the genera bacillus, escherichia, klebsiella, micrococcus, pseudomonas, salmonella, sarcina, shigella, staphylococcus and streptococcus were isolated from gills and guts of fresh water fish collected from wetland fisheries around calcutta, india, contaminated with mercury compounds. the total number of bacteria, as well as hg-resistant bacteria, were always higher in guts than gills. bottom-dwelling fish contained higher number of bacteria, including hg-re ... | 1997 | 15093380 |
| is aspartate 52 essential for catalysis by chicken egg white lysozyme? the role of natural substrate-assisted hydrolysis. | the chicken and goose egg white lysozymes (chewl and goewl) are homologues, but differ in substrate specificity. chewl catalyzes the hydrolysis of the glycosidic bonds of bacterial peptidoglycans and chitin-derived substrates, while goewl is specific for bacterial peptidoglycans. the active-site aspartate 52 residue of chewl, which is postulated to stabilize the oxocarbenium ion intermediate, has no counterpart in goewl. the substrate specificity of the d52a chewl mutant was compared with those ... | 1996 | 8639670 |
| abomasal bloat associated with sarcina-like bacteria in goat kids. | an epizootic of abdominal tympany in goat kids as a result of abomasal bloat associated with a short duration of clinical signs was fatal in over 200 kids. histologic examination of sections of abomasum revealed high numbers of bacteria that were morphologically identical to sarcina sp. sarcina sp are anaerobic, gas-producing organisms that could cause abomasal bloat. other reports have proposed that abomasal bloat is caused by abnormal abomasal flora; we propose that in the goat kids reported h ... | 1996 | 8870748 |
| sensitivity and specificity of in situ bacterial chain reaction (bcr) in detecting sparse human tumor cells in peripheral blood. | we report here the development of bacterial chain reaction (bcr), a system using micro organisms as nanodevices to amplify and visualize signals from molecular bioprobes such as antibodies, binding proteins, lectins, and oligonucleotides. unlike conventional enzyme-linked amplification systems in which the amount of enzyme is a constant parameter, in the bcr an enzyme (penicillinase) is used to trigger a proliferative chain reaction producing an exponential increase in enzyme. the detection limi ... | 1996 | 8954086 |
| two flavones from artemisia giraldii and their antimicrobial activity. | two new flavones, 4',6,7-trihydroxy-3',5'-dimethoxy-flavone (2) and 5',5- dihydroxy-3',4',8-trimethoxyflavone (3) were isolated from artemisia giraldii and their structures were identified by spectroscopic methods. these two new flavones showed antibiotic activity against staphylococcus aureus, sarcina lutea, escherichia coli, pseudomonas aeruginosa, proteus sp. aspergillus flavus, and trichoderma viride. | 1996 | 8657751 |
| [determination of radioresistance of microbes of different species irradiated separately and jointly]. | radioresistance of lactose positive and lactose negative esch. coli, lactose positive esch. coli and proteus, yellow sarcina and white staphylococcus, white and pink yeasts, white and yellow staphylococcus was studied after irradiation and seeding on hard media using separate and combined methods. in most cases radioresistance of the microbes did not depend of exposure conditions. however, lactose positive esch. coli irradiated with proteus increased proteus radioresistance to its own standard. ... | 1996 | 8696486 |
| influence of fowl uropygial gland and its secretory lipid components on growth of skin surface bacteria of fowl. | bacterial species, which occur on the breast skin surface of adult (1 year old) white leghorn fowl with intact uropygial gland, were identified as : staphylococcus epidermidis, sarcina lutea, streptomyces sp. and a facultative diphtheroid belonging to the genus corynebacterium; s. epidermidis being the most predominant one. two species of bacteria, namely, staphylococcus aureus and proteus sp. were shown to colonize the skin surface after 60 days of captivity. extirpation of uropygial gland caus ... | 1996 | 8698407 |
| re: b. l. røder, et al.: dicloxacillin and flucloxacillin: pharmacokinetics, protein binding and serum bactericidal titers in healthy subjects after oral administration (infection 23 [1995] 107-112) | 1996 | 8811373 | |
| biological studies on 1,2-benzisothiazole derivatives v. antimicrobial properties of n-alkanoic, n-arylalkanoic and n-aryloxyalkanoic derivatives of 1,2-benzisothiazolin-3-one: qsar study and genotoxicity evaluation. | n-alkanoic, n-arylalkanoic and n-aryloxyalkanoic acids of 1,2-benzisothiazolin-3-one, esters and amides of n-arylalkanoic and n-aryloxyalkanoic acids and 1,1-dioxide derivatives of n-arylalkanoic acids and esters were investigated in vitro antimicrobial activity. n-arylalkanoic and n-aryloxyalkanoic acids (compounds 4-12) and their esters and amides (compounds 13-26) exhibited a good antimicrobial activity against gram positive bacteria, with several compounds showing potencies 10-20 times highe ... | 1996 | 8765672 |
| antibacterial and immunostimulating casein-derived substances from milk: casecidin, isracidin peptides. | apart from immunoglobulin a and g antibodies and plasma cells, milk also contains antibiotic/host protective peptides that are of value not only for maintenance of its nutritional integrity but also for protection of the newborn and, possibly, protection of the lactating mother. among the first such peptides identified with casecidin; following chymosin digestion of casein at ph 6 or 7, casecidin inhibited in vitro staphylococci, sarcina, bacillus subtilis, diplococcus pneumoniae and streptococc ... | 1996 | 8603791 |
| in vitro antimicrobial activity of pistacia lentiscus l. extracts: preliminary report. | the in vitro antimicrobial activity of pistacia lentiscus l. extracts was determined. pistacia lentiscus l. extracts were tested on bacteria (sarcina lutea, staphylococcus aureus and escherichia coli) and fungi (candida albicans, candida parapsilosis, torulopsis glabrata and cryptococcus neoformans). of the different plant extractions, decoctions showed the best antibacterial activity, but the activity against fungal cells appears to be much more interesting. | 1996 | 8808717 |
| membrane lipid alkyl chain motional dynamics is conserved in sarcina ventriculi despite ph-induced adaptative structural modifications including alkyl chain tail to tail coupling. | sarcina ventriculi, an anaerobic gram-positive bacterium, adapts to increasing temperature, the presence of organic solvents, or the lowering of the ph of its growth medium by joining the tails of membrane lipids from opposite sides of the bilayer, forming transmembrane, bifunctional fatty acid species. since this is done to offset the increase in membrane mobility caused by these perturbations, it is of interest to determine whether the motional (dynamic) properties of membrane lipid alkyl chai ... | 1995 | 7547938 |
| insecticides: their effect on microorganisms and persistence in rice soil. | a field experiment was conducted to investigate the effect of four insecticides, hch, phorate, carbofuran and fenvalerate, at recommended doses on the preponderance of bacteria, actinomycetes and fungi. we also measured the persistence of the insecticides in the rhizosphere soil of rice. hch and fenvalerate stimulated the proliferation of all of the microorganisms significantly. phorate increased the population of bacteria and actinomycetes. carbofuran accentuated the preponderance of actinomyce ... | 1995 | 7541287 |
| transformation of microorganisms with the plasmid vector with the replicon from pac1 from acetobacter pasteurianus. | a number of gram-negative and gram-positive bacteria species was screened for the expression of the gram-negative plasmid pack5 and pact72 with replicon of pac1 plasmid from acetobacter pasteurianus. as was described previously, both plasmids were expressed in escherichia coli, acetobacter pasteurianus, acetobacter aceti, shigella spp. and citrobacter spp. expressions of plasmids were successful in twelve species tested, comamonas terrigena, salmonella typhimurium, serratia marcescens, bacillus ... | 1995 | 7832808 |
| laboratory and clinical stain removal evaluations of two tartar control dentifrices. | the purpose of this research was to evaluate colgate original tartar control dentifrice (tc) and new colgate micro cleansing tartar control (mctc) dentifrice in a laboratory and clinical study for their ability to remove induced stain. in the laboratory study, polished and etched bovine enamel specimens were stained for 4 days with a coffee, tea, and mucin, sarcina lutea tartox culture in trypticase soy broth. sixteen specimens were then brushed for 200 strokes with a sodium carboxymethyl cellul ... | 1995 | 8624234 |
| a new myxococcus xanthus gene cluster for the biosynthesis of the antibiotic saframycin mx1 encoding a peptide synthetase. | the gene cluster for the biosynthesis of the heterocyclic quinone antibiotic saframycin mx1 of myxococcus xanthus dm504/15 was inactivated and tagged by tn5 insertions. the tagged genes were cloned in escherichia coli and used to select overlapping cosmid clones spanning 58 kb of the m. xanthus genome. gene disruption experiments defined a > or = 18 kb contiguous dna region involved in saframycin biosynthesis. sequencing of part of this region revealed a large orf containing two 600-amino-acid d ... | 1995 | 7551044 |
| a new in-vitro model to investigate antibiotic penetration of the intervertebral disc. | we have studied the ability of a range of antibiotics to penetrate intervertebral disc tissue in vitro, using a mouse disc model. equilibrium concentrations of antibiotics incorporated into the entire disc were determined by bioassay using a microbial growth-inhibition method. uptake was significantly higher with positively-charged aminoglycosides compared with negatively-charged penicillins and cephalosporins. uncharged ciprofloxacin showed an intermediate degree of uptake. our results support ... | 1995 | 7593116 |
| a mathematical model explaining the molecular weights and distribution of very long chain dicarboxylic acids formed during the adaptive response of sarcina ventriculi. | a simple mathematical model is presented to explain a recent new discovery of an unusual membrane adaptive response in sarcina ventriculi. in this response, this organism synthesizes very long chain alpha, omega-dicarboxylic acids ranging from 28 to 36 carbon atoms in length. the distribution of chain lengths of the new fatty acid species is not consistent with de novo synthesis but suggests elaboration from the existing regular-chain fatty acids by a coupling process. here, we demonstrate, usin ... | 1995 | 7891453 |
| 6-hydroxymanzamine a and 3,4-dihydromanzamine a, new alkaloids from the okinawan marine sponge amphimedon sp. | two new beta-carboline alkaloids, 6-hydroxymanzamine a [1] and 3,4-dihydromanzamine a [2], have been isolated from the okinawan marine sponge amphimedon sp. and their structures elucidated on the basis of nmr spectral data. | 1994 | 7714542 |
| evaluation of lung tissue and hilar lymph node concentrations of azithromycin. | since it is believed that most infections occur in the interstitial fluid, it has been suggested to measure the concentration of antimicrobial agents in pulmonary lymph that reflects the extracellular, extra-blood-vascular milieu in the lung. the aim of this study was to investigate the lung tissue and pulmonary lymph node penetration of azithromycin after 500 mg daily oral administration for three days in patients undergoing open thoracotomy. at the end of the treatment, each subject was assign ... | 1994 | 8004364 |
| 16s ribosomal dna sequences of anaerobic cocci and proposal of ruminococcus hansenii comb. nov. and ruminococcus productus comb. nov. | the 16s ribosomal dna sequences of representative members of the family peptococcaceae were determined. the members of the family examined were divided into the following four phylogenetic groups: peptococcus niger atcc 27731t (t = type strain), the sarcina-peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. peptococcus niger, the type species of the family, was not related to other members of the family. peptostreptococcus anaerobius atcc 2 ... | 1994 | 8123556 |
| structures and stereochemistry of the very long alpha, omega-bifunctional alkyl species in the membrane of sarcina ventriculi indicate that they are formed by tail-to-tail coupling of normal fatty acids. | in a previous study, we demonstrated that sarcina ventriculi is capable of adjusting to alterations in environmental conditions (such as increase in temperature, lowering of ph, or addition of exogenous organic solvents) by the synthesis of a family of alpha, omega-dicarboxylic acids ranging from 28 to 36 carbons long (jung, s., et al. 1993. j. biol. chem. 268: 2828-2835). the chain lengths and relative abundance of the very long dicarboxylic acids found in s. ventriculi suggest that they may be ... | 1994 | 7868972 |
| [blood serum and milk serum levels of sodium benzylpenicillin after intravenous regional antibiosis in surgery of the claw area in cattle]. | an intravenous regional antibiosis using 10 mega i.u. sodium-benzylpenicillin was carried out during surgery in 14 dairy cows affected with spontaneous occurring digital diseases. blood serum and milk serum levels were determined over a period of 48 hours by means of a micro agar diffusion method using sarcina lutea stamm bga berlin as a test germ. even before opening of the rubber tourniquet, penicillin traces were detectable in the blood serum of 6 cows. maximal concentrations ranging from 0.4 ... | 1994 | 7945183 |