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serological and molecular prevalence of canine vector-borne diseases (cvbds) in korea.previous surveys in dogs from korea indicated that dogs are exposed to a variety of vector- borne pathogens, but perception for a nation-wide canine vector-borne disease (cvbd) occurrence has been missing. we report here results of both serological and molecular prevalence studies for major cvbds of dogs from all over the south korean peninsula except for jeju island.201728298245
association of ehrlichia canis, hemotropic mycoplasma spp. and anaplasma platys and severe anemia in dogs in thailand.canine tick-borne bacteria; ehrlichia canis, hemotropic mycoplasma spp. and anaplasma spp., are organisms transmitted by rhipicephalus sanguineus ticks. however, only a few clinical studies evaluating dogs infected with these organisms and anemia condition have been published. in this study, the potential tick-borne bacteria linked to anemia were investigated in eighty-one blood samples selected from anemic dogs using a broad range nested-pcr of the 16s rrna gene. positive results were shown in ...201728284610
species of ticks and carried pathogens in owned dogs in spain: results of a one-year national survey.this study presents the results of a national survey in spain on the distribution of ticks on owned dogs, their phenology and the associated pathogens over one year. in the study, 1628 adult ticks were collected on 660 dogs presented to 26 veterinary practices, of which 507 dogs (76.8%) carried at least one adult tick. the primary species of ticks were rhipicephalus sanguineus s.l. (53%), dermacentor reticulatus (9%), ixodes ricinus (9%), and i. hexagonus (4%). parasitism by two species of these ...201728188085
canine tick-borne pathogens in cyprus and a unique canine case of multiple co-infections.canine tick-borne pathogens such as ehrlichia canis and hepatozoon canis are widespread in the mediterranean basin but have never been reported or investigated in cyprus. we describe herein the presence of canine tick-borne pathogens in three dogs with clinical signs compatible with vector-borne diseases from paphos area of cyprus. molecular and phylogenetic analysis revealed the presence of e. canis, anaplasma platys, h. canis, babesia vogeli and mycoplasma haemocanis in cyprus. one dog co-infe ...201728089651
anaplasma platys in dogs from uruguay.anaplasmataceae family members include vector-borne bacteria of veterinary importance that may also affect humans. ehrlichia canis and anaplasma platys are the main members of this family detected in dogs worldwide. in uruguay there are not many published studies on tick-borne pathogens affecting dogs, the only haemoparasite molecularly confirmed in dogs, is the piroplasm rangelia vitalii. the aim of the present work was to detect the presence of a. platys and e. canis in dogs and dogs-associate ...201727908772
first molecular detection of tick-borne pathogens in dogs from jiangxi, china.in this study, blood samples obtained from 162 dogs in jiangxi, china, were employed in molecular screening of canine tick-borne pathogens by pcr and sequencing. babesia spp. gene fragment was detected in 12 (7.41%) dogs. all samples were negative for hepatozoon spp., ehrlichia canis, coxiella spp., borrelia spp., rickettsia spp. and anaplasma platys. species-specific pcr analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by babesia canis vogeli and babesia gibsoni, respe ...201727890889
initial development and preliminary evaluation of a multiplex bead assay to detect antibodies to ehrlichia canis, anaplasma platys, and ehrlichia chaffeensis outer membrane peptides in naturally infected dogs from grenada, west indies.tick-borne bacteria, ehrlichia canis, anaplasma platys, and ehrlichia chaffeensis are significant pathogens of dogs worldwide, and coinfections of e. canis and a. platys are common in dogs on the caribbean islands. we developed and evaluated the performance of a multiplex bead-based assay to detect antibodies to e. canis, a. platys, and e. chaffeensis peptides in dogs from grenada, west indies, where e. canis and a. platys infections are endemic. peptides from outer membrane proteins of p30 of e ...201727852813
the eurasian otter (lutra lutra) as a potential host for rickettsial pathogens in southern italy.canine monocytic ehrlichiosis and rickettsiosis are zoonotic tick-borne diseases of canids caused by the intracellular obligate bacteria ehrlichia canis and rickettsia species respectively. in this study, we investigated using standard and real-time pcr and sequencing, the occurrence and molecular characterization of e. canis and rickettsia species in the eurasian otter (lutra lutra) from the southern italian population. samples were screened by using molecular assays also for neospora caninum, ...201728267780
molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease.the spleen is a highly perfused organ involved in the immunological control and elimination of vector-borne pathogens (vbp), which could have a fundamental role in the pathogenesis of splenic disease. this study aimed to evaluate certain vbp in samples from dogs with splenic lesions.201728285583
molecular detection of hepatozoon spp. and cytauxzoon sp. in domestic and stray cats from madrid, spain.different species of apicomplexan protozoans of the genera hepatozoon and cytauxzoon can infect domestic cats, but their epidemiology and clinical relevance are not fully understood. the aim of this study was to assess the molecular prevalence of hepatozoon spp. and cytauxzoon spp. and to identify associated risk factors and clinical and laboratory abnormalities in a population of cats from madrid, spain.201728285590
serological detection of antibodies to anaplasma spp., borrelia burgdorferi sensu lato and ehrlichia canis and of dirofilaria immitis antigen in dogs from costa rica.in a study in costa rica 314 serum samples from dogs throughout all seven provinces were tested using a commercial kit for the detection of circulating antibodies against anaplasma spp., borrelia burgdorferi sensu lato and ehrlichia canis, and of circulating antigen of dirofilaria immitis. a total of 6.4% (20/314) and 38.2% (120/314) were positive for anaplasma spp. (an) and e. canis (ec) antibodies. overall, 8.0% (25/314) were positive for d. immitis (di) antigen. one single dog reacted positiv ...201728288773
novel genotype of ehrlichia canis detected in samples of human blood bank donors in costa rica.this study focuses on the detection and identification of dna and antibodies to ehrlichia spp. in samples of blood bank donors in costa rica using molecular and serological techniques. presence of ehrlichia canis was determined in 10 (3.6%) out of 280 blood samples using polymerase chain reaction (pcr) targeting the ehrlichial dsb conserved gene. analysis of the ehrlichial trp36 polymorphic gene in these 10 samples revealed substantial polymorphism among the e. canis genotypes, including diverge ...201727682202
performance of leishmania braziliensis enolase protein for the serodiagnosis of canine and human visceral leishmaniosis.in the present study, leishmania braziliensis enolase was cloned and the recombinant protein (renolase) was evaluated for the serodiagnosis of canine and human visceral leishmaniosis (vl). for the canine vl diagnosis, this study examined serum samples of leishmania infantum-infected dogs, from non-infected animals living in endemic or non-endemic areas of leishmaniosis, as well as those from leish-tec(®)-vaccinated dogs and trypanosoma cruzi or ehrlichia canis experimentally infected animals. fo ...201728385540
clinical and hematobiochemical response in canine monocytic ehrlichiosis seropositive dogs of punjab.as in india especially, the punjab state sero-prevalence and distribution of ehrlichiosis in relation to clinico-hematobiochemical response remains largely unexplored. thus, this study was designed to determine the prevalence of vector (tick)-borne tropical canine pancytopenia caused by ehrlichia canis through enzyme labeled immunocomb(®) (ic) assay in dogs from in and around ludhiana, punjab. correlation of prevalence was made with various clinico-hematobiochemical parameters.201728344412
ferritin 1 silencing effect in rhipicephalus sanguineus sensu lato (acari: ixodidae) during experimental infection with ehrlichia canis.rhipicephalus sanguineus sensu lato (s.l.) is a very common ectoparasite of domestic dogs able to transmit several pathogens of human and veterinary importance. tick infestations and tick-borne diseases (tbds) remain a serious and persistent problem, due to the lack of efficient control measures. it is therefore vital that novel approaches to control are pursued. whilst vaccination is recognised as a potential control method to reduce tick infestation, no anti-r. sanguineus vaccine is available. ...201727825659
pathology of dogs in campo grande, ms, brazil naturally co-infected with leishmania infantum and ehrlichia canis.different parasites that commonly occur concomitantly can influence one another, sometimes with unpredictable effects. we evaluated pathological aspects of dogs naturally co-infected with leishmania infantum and ehrlichia canis. the health status of the dogs was investigated based on histopathological, hematological and biochemical analyses of 21 animals infected solely with l. infantum and 22 dogs co- infected with l. infantum and e. canis. the skin of both groups showed chronic, predominantly ...201725517530
the prevalence of anaplasma platys and a potential novel anaplasma species exceed that of ehrlichia canis in asymptomatic dogs and rhipicephalus sanguineus in taiwan.canine anaplasmosis is regarded as an infection by anaplasma platys rather than zoonotic anaplasma phagocytophilum in subtropical areas based on the assumption that the common dog tick species is rhipicephalus sanguineus, which transmits e. canis and presumably a. platys. we investigated asymptomatic dogs and dog ticks from 16 communities in nantou county, taiwan to identify common dog tick species and to determine the prevalence of anaplasma and ehrlichia spp. of total 175 canine blood samples ...201728781347
molecular investigation and phylogeny of anaplasmataceae species infecting domestic animals and ticks in corsica, france.corsica is a french island situated in the mediterranean sea. the island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. the family anaplasmataceae is a member of the order rickettsiales; it includes the genera anaplasma, ehrlichia, neorickettsia and wolbachia. anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera anaplasma and ehrlichia. the a ...201728645313
a new pcr assay for the detection and differentiation of babesia canis and babesia vogeli.babesia spp. are globally distributed tick-borne protozoan parasites that infect the red blood cells of a wide range of vertebrate hosts, including humans. diagnosis of babesiosis is often impeded by the transient presence of the parasites in peripheral blood, as well as by their pleomorphic nature. given the reports of an expanding and, in some cases, sympatric geographical distribution of babesia canis and babesia vogeli in dogs and associated vectors, in europe, the development of time-effici ...201728739301
a pcr survey of vector-borne pathogens in different dog populations from turkey.in the present study, a total of 192 blood samples were collected from pet dogs, kennel dogs and shepherd dogs in konya district, turkey, and tested by specific pcr for the presence of vector-borne pathogens. several pathogens were identified, most of which can cause substantial morbidity in dogs. pcr results revealed that 54 (28.1%) dogs were infected with one or more pathogens. positive results were obtained for babesia spp. in 4 dogs (2.1%), hepatozoon spp. in 8 dogs (4.2%) and mycoplasma spp ...201728682765
molecular detection of vector-borne pathogens in dogs and cats from qatar.vector-borne diseases (vbds) have been increasingly reported in dogs and cats worldwide. however, no data are currently available regarding canine and feline vbds in qatar and limited information is available from other persian gulf countries.201728633666
molecular detection of tick-borne pathogens in wild red foxes (vulpes vulpes) from central italy.spleen samples from 153 red foxes, shot during regular hunting season in the province of pisa (central italy), were examined to detect dna of anaplasma phagocytophilum, ehrlichia canis, coxiella burnetii, francisella tularensis, hepatozoon canis and babesia sp./theileria sp. dna of vector-borne pathogens was detected in 120 (78.43%; 95% ci: 71.06-84.66%) foxes. specifically, 75 (49%; 95% ci: 40.86-57.22%) animals scored pcr-positive per h. canis, 68 (44.44%; 95% ci: 36.42-52.69%) for e. canis, 3 ...201728502642
seroprevalence and risk factors associated with ehrlichia canis, anaplasma spp., borrelia burgdorferi sensu lato, and d. immitis in hunting dogs from southern italy.canine vector-borne diseases (cvbds) are caused by a range of pathogens transmitted to dogs by arthropods. the present study investigates ehrlichia canis, anaplasma spp., borrelia burgdorferi sensu lato, and dirofilaria immitis seroprevalences in hunting dogs from southern italy. dogs (no. 1335) were tested using a commercial in-clinic enzyme-linked immunosorbent assay kit. odds ratios (ors) were calculated by logistic regression analysis to identify risk factors. overall, 138/1335 dogs (10.3%) ...201728776227
occurrence of <i>coxiella burnetii</i>, <i>ehrlichia canis</i>, <i>rickettsia</i> species and <i>anaplasma phagocytophilum</i>-like bacterium in ticks collected from dogs and cats in south africa.ticks are major vectors of arthropod-borne infections and transmit a wide variety of zoonotic pathogens. this study was conducted mainly to determine the occurrence of canine tick-borne bacterial and rickettsial pathogens especially those with zoonotic potential. we examined 276 rhipicephalus sanguineus, 38 haemaphysalis elliptica and 4 amblyomma hebraeum ticks from 90 dogs and 4 cats from the free state, kwazulu-natal, north west and mpumalanga provinces. dna of coxiella burnetii (41%), ehrlich ...201728582983
prevalence and geographic distribution of vector-borne pathogens in apparently healthy dogs in croatia.vector-borne pathogens (vbps) are a group of globally extended and quickly spreading pathogens that are transmitted by various arthropod vectors. the aim of the present study was to investigate the seroprevalence against babesia canis, anaplasma phagocytophilum, borrelia burgdorferi sensu lato, leishmania infantum, dirofilaria immitis, and ehrlichia canis in dogs in croatia. we investigated 435 randomly selected apparently healthy dogs in 13 different locations of croatia for antibodies to b. ca ...201728448211
molecular detection of tick-borne pathogen diversities in ticks from livestock and reptiles along the shores and adjacent islands of lake victoria and lake baringo, kenya.although diverse tick-borne pathogens (tbps) are endemic to east africa, with recognized impact on human and livestock health, their diversity and specific interactions with tick and vertebrate host species remain poorly understood in the region. in particular, the role of reptiles in tbp epidemiology remains unknown, despite having been implicated with tbps of livestock among exported tortoises and lizards. understanding tbp ecologies, and the potential role of common reptiles, is critical for ...201728620610
vector-borne pathogens in stray dogs in northeastern turkey.this experiment was carried out to attain prevalence and molecular characterization of pathogens causing canine vector-borne diseases (cvbds) including babesiosis, hepatozoonosis, leishmaniasis, filariosis (dirofilaria immitis, dirofilaria repens, and acanthocheilonema reconditum), ehrlichiosis (ehrlichia canis), and anaplasmosis (anaplasma platys) in stray dogs. the study material consisted of 133 asymptomatic female (n = 96) and male (n = 37) stray dogs (≤1 year old, n = 16 and 1-6 years old, ...201728632488
new wide dynamic range assays for quantification of anti-leishmania igg2 and iga antibodies in canine serum.the aims of this study were (1) to develop and validate time resolved-immunofluorometric assays for the detection of anti-leishmania igg2 and iga antibodies in canine serum and (2) to evaluate the ability of these assays to quantify different amounts of anti-leishmania antibodies in leishmania-seronegative and seropositive dogs, determined by a commercial elisa assay, and between different clinical stages according to leishvet guidelines. the analytical validation showed that the assays had a go ...201728669382
implication, clinical and biological impact of vector-borne haemopathogens in anaemic dogs in france: a prospective study.to prospectively describe the clinical and biological impact of vector-borne haemopathogens in anaemic dogs in france and occurrence of haemolysis.201728543264
molecular survey of anaplasma platys and ehrlichia canis in dogs from campo grande, mato grosso do sul, brazil.this study investigated the frequency of infection by anaplasma platys and ehrlichia canis in dogs submitted to animal health centers in campo grande, state of mato grosso do sul, brazil. e. canis and a. platys showed infection frequencies of 55.75% and 16.96%, respectively. the identity of the two species was confirmed by dna sequencing.201728423085
seropositivity for rickettsia spp. and ehrlichia spp. in the human population of mato grosso, central-western brazil.the epidemiology of rickettsia and ehrlichia species infection is underestimated in mato grosso state.201728700062
molecular prevalence and haemato-biochemical profile of canine monocytic ehrlichiosis in dogs in and around hisar, haryana, india.the present study was planned to investigate the molecular prevalence of canine monocytic ehrlichiosis (cme) in dogs in and around hisar and to evaluate the haemato-biochemical profile for its better management. a total of 60 dogs presented to medicine section, tvcc, luvas, hisar with the history of naturally acquired tick infestation and clinical signs consistent with cme were screened on the basis of blood smear examination, followed by molecular detection by nested pcr assay targeting a porti ...201728848253
cerebral haemorrhage as a clinical manifestation of human ehrlichiosis.a 16-year-old young man presented to the emergency room with new-onset generalised tonic-clonic seizures. examination showed a glasgow score of 13 and predominantly crural left hemiparesis. imaging demonstrated a right frontoparietal haemorrhage of non-vascular origin with perilesional oedema. surgical drainage was carried out, but rebleeding occurred within 24 hours following surgery, and again 1 week after discharge. on reinterrogation and examination, ehrlichia canis infection was suspected a ...201728751428
high-dose filgrastim treatment of nonregenerative pancytopenia associated with chronic canine ehrlichiosis.to report the management and outcome of a dog with canine monocytic ehrlichiosis and nonregenerative pancytopenia, with high doses of filgrastim.201728750787
ehrlichia canis and rickettsia spp. in dogs from urban areas in paraiba state, northeastern brazil.the aims of our study was to identify ehrlichia canis and antibodies against rickettsia spp. belonging to the spotted fever group (sfg) in dogs sampled from paraiba state, northeastern brazil. blood and serum samples collected by convenience from dogs in urban areas of five municipalities were analyzed by real-time pcr for the detection of e. canis dna and by immunofluorescence assay test (ifat) for the identification of antibodies against rickettsia rickettsii, r. felis, r. parkeri, r. amblyomm ...201728658415
therapeutic management of canine ehrlichiosis with aid of blood transfusion: a case report.an adult female labrador retriever dog was presented at referral veterinary polyclinic, ivri in lateral recumbency with history of anorexia, high fever, severe panting, heavy tick infestation, and not responding to cephalosporins and fluoroquinolones since last 1 week. clinical examination showed pale conjunctiva, enlarged popliteal lymph node however, electrocardiogram (ecg) report found to be normal. haematological finding showed marked anaemia and thrombocytopenia. parasitological examination ...201728615848
occurrence of leishmania infantum in the central nervous system of naturally infected dogs: parasite load, viability, co-infections and histological alterations.zoonotic visceral leishmaniasis is caused by the protozoan leishmania infantum and little is known about the occurrence and pathogenesis of this parasite in the cns. the aims of this study were to evaluate the occurrence, viability and load of l. infantum in the cns, and to identify the neurological histological alterations associated with this protozoan and its co-infections in naturally infected dogs. forty-eight leishmania-seropositive dogs from which l. infantum was isolated after necropsy w ...201728419136
cardiac troponin i concentrations, electrocardiographic and echocardiographic variables remained unchanged in dogs experimentally infected with ehrlichia canis.canine monocytic ehrlichiosis (cme, ehrlichia canis) has occasionally been associated with myocardial injury. the aim of the present study was to serially measure and evaluate cardiac troponin i (ctni) concentrations in dogs with experimentally induced acute and subclinical cme and to evaluate potential associations between ctni concentration and an array of echocardiographic and electrocardiographic parameters. serum ctni concentration and simultaneous echocardiographic and electrocardiographic ...201627810200
iron metabolism and oxidative profile of dogs naturally infected by ehrlichia canis: acute and subclinical disease.the aim of this study was to evaluate the oxidant profile and iron metabolism in serum of dogs infected by ehrlichia canis. banked sera samples of dogs were divided into two groups: negative control (n = 17) and infected by e. canis on acute (n = 24), and subclinical (n = 18) phases of the disease. the eritrogram, leucogram, and platelet counts were evaluate as well as iron, ferritin, and transferrin levels, latent iron binding capacity (libc), and transferrin saturation index (tsi) concentratio ...201626724737
evaluation of clinical, biochemical and haematological markers in natural infection of canine monocytic ehrlichiosis.caanine monocytic ehrlichiosis caused by ehrlichia canis has gained wider significance owing to its potential to inflict significant deleterious effect on the health of companion animals. in the present study, 46 confirmed ehrlichiosis positive dogs were evaluated for the alterations in clinical, haematological and biochemical attributes. depression, anorexia, pyrexia, anaemia, weakness, jaundice, melana, vomition and diarrhoea were the main clinical symptoms onserved. haematological alterations ...201627876946
molecular evidence of vector-borne pathogens in dogs and cats and their ectoparasites in algiers, algeria.in algeria, only limited information is currently available on the prevalence of emergent canine and feline vector-borne diseases. the aim of the present work was to detect by qpcr vector-associated bacteria in stray dogs and cats and their ectoparasites from algiers. 18/117 (15.38%) dogs and 2/107 (1.87%) cats were positive for at least one vector-borne agent. coxiella burnetii and bartonella henselae were identified in 1/117 (0.85%) dog individually. ehrlichia canis dna was detected in 17/117 ...201627012917
genetic diversity of ehrlichia canis strains from naturally infected dogs in rio de janeiro, brazil.the aim of this study was to characterize ehrlichia canis strains from naturally infected dogs in rio de janeiro, brazil. in addition, all the clinical and hematological findings observed in these dogs were reported. pcr targeting the 16s rrna gene was used for diagnostic purposes, and the trp19 and trp36 genes were sequenced to evaluate the genetic diversity. fifteen samples were positive for e. canis. the polymerase chain reaction for the trp19 gene resulted in 11 amplicons (11/15), which were ...201625271448
detection of genotype-specific ehrlichia canis exposure in brazilian dogs by trp36 peptide elisa.we recently characterized a novel genotype of ehrlichia canis based on the tandem repeat (tr) sequence of the trp36 gene in brazil. the tr amino acid sequence of the brazilian (br) genotype (asvvpeae) was divergent from the previously described us genotype (tedsvsapa) of e. canis. in this study, we developed an elisa based on trp36 tr synthetic peptides from both br and us e. canis trp36 genotypes to serologically detect and distinguish infections caused by these genotypes. sera from 30 brazilia ...201626482949
a novel ehrlichial agent detected in tick in french polynesia.ticks are hematophageous arthropods that are known to host and transmit miscellaneous pathogens including zoonotic bacteria. the aim of this study was to investigate the presence of tick-associated microorganisms in tahiti, french polynesia with molecular tools. a total of 658 ticks from two species including rhipicephalus sanguineus s.l. and rh. annulatus were collected with forceps on dogs and cattle respectively, or with a flag on pasture in several locations of tahiti in 2013. two rickettsia ...201627522945
serology, molecular detection and risk factors of ehrlichia canis infection in dogs in costa rica.a cross-sectional study combining different serological and molecular techniques for the detection of ehrlichia species in dogs and their ticks was carried out with data from all regions of costa rica. a seroprevalence of 32.1% (131/408), and infection with e. canis of 3.2% (13/407) was found, whereas 6.9% (9/130) of ticks attached to the dogs were pcr positive to e. canis. higher prevalences were found outside the greater metropolitan area (gma). risk factors associated with e. canis seropositi ...201627440400
interstitial pneumonia and pulmonary hypertension associated with suspected ehrlichiosis in a dog.in dogs with canine monocytic ehrlichiosis (cme), respiratory signs are uncommon and clinical and radiographic signs of interstitial pneumonia are poorly described. however, in human monocytic ehrlichiosis, respiratory signs are common and signs of interstitial pneumonia are well known. pulmonary hypertension (ph) is classified based on the underlying disease and its treatment is aimed at reducing the clinical signs and, if possible, addressing the primary disease process. ph is often irreversib ...201627388158
rangelia vitalii, babesia spp. and ehrlichia spp. in dogs in passo fundo, state of rio grande do sul, brazil.pathogens transmitted by ticks are an emerging problem worldwide, this study aimed to diagnose the causal agents of infection in dogs presenting suspected hemoparasitoses. fifty-eight dogs with clinical signs such as depression, hemorrhagic diathesis and fever were evaluated regarding clinical presentation, hemogram, blood smears and serological tests, using the indirect immunofluorescence method for the agents babesia vogeli and ehrlichia canis and conventional pcr for babesia spp. (gene 18s rr ...201627334817
rangelia vitalii, babesia spp. and ehrlichia spp. in dogs in passo fundo, state of rio grande do sul, brazil.pathogens transmitted by ticks are an emerging problem worldwide, this study aimed to diagnose the causal agents of infection in dogs presenting suspected hemoparasitoses. fifty-eight dogs with clinical signs such as depression, hemorrhagic diathesis and fever were evaluated regarding clinical presentation, hemogram, blood smears and serological tests, using the indirect immunofluorescence method for the agents babesia vogeli and ehrlichia canis and conventional pcr for babesia spp. (gene 18s rr ...201627304518
identification of vector-borne pathogens in dogs and cats from southern brazil.dogs and cats are often infected with vector-borne pathogens and play a crucial role as reservoirs and hosts in their life cycles. the aim of the present study was to investigate the occurrence of vector-borne pathogens among dogs and cats in the northwestern region of rio grande do sul (rs) state, brazil. one hundred and ten blood samples were collected from dogs (n=80) and cats (n=30). laboratory analysis were carried out through stained blood smears, indirect enzyme-linked immunosorbent assay ...201627266811
quantitative and qualitative leukocyte abnormalities in dogs with experimental and naturally occurring acute canine monocytic ehrlichiosis.canine monocytic ehrlichiosis (cme) is one of the most important tick-borne diseases worldwide. cytopenias have been observed in both acute (nonmyelosuppressive) and chronic (myelosuppressive) cme; however, leukocyte abnormalities and indices have been incompletely described in dogs with acute cme.201627142117
minimum infection rate of ehrlichia minasensis in rhipicephalus microplus and amblyomma sculptum ticks in brazil.a new genotype phylogenetically close to ehrlichia canis named as ehrlichia minasensis was identified infecting cattle and deer in canada, as well as rhipicephalus microplus ticks and cattle in brazil. although it was detected in r. microplus, little is known about the epidemiology of this ehrlichiosis, especially in other tick species. this study evaluated the minimum infection rate of e. minasensis in amblyomma sculptum and r. microplus ticks from locations where naturally infected cattle were ...201627084673
acute phase protein and antioxidant responses in dogs with experimental acute monocytic ehrlichiosis treated with rifampicin.there is currently lack of information on the changes of acute phase proteins (app) and antioxidant markers and their clinical relevance as treatment response indicators in canine monocytic ehrlichiosis (cme). the objective of this study was to investigate the patterns of c-reactive protein (crp), haptoglobin (hp), ferritin and paraoxonase-1 (pon-1) during treatment of dogs with acute cme with rifampicin. blood serum samples from ten beagle dogs with experimental acute cme were retrospectively e ...201626854345
evaluation of two recombinant leishmania proteins identified by an immunoproteomic approach as tools for the serodiagnosis of canine visceral and human tegumentary leishmaniasis.serological diagnostic tests for canine and human leishmaniasis present problems related with their sensitivity and/or specificity. recently, an immunoproteomic approach performed with leishmania infantum proteins identified new parasite antigens. in the present study, the diagnostic properties of two of these proteins, cytochrome c oxidase and ige-dependent histamine-releasing factor, were evaluated for the serodiagnosis of canine visceral (cvl) and human tegumentary (htl) leishmaniasis. for th ...201626790739
serological evidence of exposure to tick-borne agents in opossums (didelphis spp.) in the state of são paulo, brazil.this work involved a serological investigation of tick-borne pathogens in opossums in eight municipalities of the state of são paulo, brazil. serum samples from 109 opossums (91 didelphis aurita and 18 didelphis albiventris) were tested to detect antibodies to rickettsia rickettsii (taiaçu strain, 1:64 cut-off) and ehrlichia canis (são paulo strain, 1:40 cut-off), by indirect immunofluorescence assay (ifa); and against borrelia burgdorferi (strain g39/40) by enzyme-linked immunosorbent assay (el ...201627276663
molecular assessment of the transplacental transmission of toxoplasma gondii, neospora caninum, brucella canis and ehrlichia canis in dogs.given the fact that numerous microbial species can be detected in pregnant female dogs, the objective of this study was to assess the transplacental transmission of brucella canis, ehrlichia canis, neospora caninum and toxoplasma gondii in stillborn puppies. this study involved 41 stillborn puppies, 78.6% of which were positive for t. gondii, 52.4% for n. caninum and 59.5% for b. canis. e. canis was not detected in any of the analyzed puppies. pregnancy is an important physiological condition fo ...201627865263
molecular survey of ehrlichia canis and coxiella burnetii infections in wild mammals of southern italy.ehrlichiosis and q fever caused by the intracellular bacteria ehrlichia canis and coxiella burnetii, respectively, are tick-borne diseases with zoonotic potential and widespread geographical distribution. this study investigated the prevalence of both infections in wild mammals in southern italy. tissue samples obtained from the red fox (vulpes vulpes), european badger (meles meles), gray wolf (canis lupus), beech marten (martes foina), and crested porcupine (hystrix cristata) were processed for ...201627535678
molecular detection of vector-borne pathogens in wild and domestic carnivores and their ticks at the human-wildlife interface.urbanization of natural areas is considered one of the causes of the current apparent emergence of infectious diseases. carnivores are among the species that adapt well to urban and periurban environments, facilitating cross-species disease transmission with domestic dogs and cats, and potentially with their owners. the prevalence of vector-borne pathogens (vbp) of zoonotic and veterinary interest was studied in sympatric wild and domestic carnivores into barcelona metropolitan area (ne spain). ...201626643497
critical analysis of vector-borne infections in dogs: babesia vogeli, babesia gibsoni, ehrlichia canis and hepatozoon canis in punjab, india.there are few published studies on various vector borne diseases of dogs in india and most depict clinical infection in dogs, diagnosed by observation of the haemopathogens in stained blood smears. this study provides the first report regarding molecular confirmation and ancestral relationship analysis of blood smears positive cases of assorted haemopathogens in punjab province of india. on blood smear examination, haemopathogens were observed in 124 out of 778 (15.95%, 95% ci: 13.53- 18.68) blo ...201627787221
molecular epidemiological surveillance to assess emergence and re-emergence of tick-borne infections in tick samples from china evaluated by nested pcrs.an investigation was performed to detect eight pathogens in ticks collected from grass tips or animals in the southern, central and northeast regions of china. dna samples extracted from ticks were collected from ten different locations in eight provinces of china and subjected to screening for tick-borne pathogens, including borrelia burgdorferi sensu lato, ehrlichia spp., rickettsia spp., babesia/theileria spp., ehrlichia ruminantium, coxiella burnetii, and francisella tularensis, using nested ...201626943995
a new leishmania-specific hypothetical protein and its non-described specific b cell conformational epitope applied in the serodiagnosis of canine visceral leishmaniasis.the serodiagnosis of canine visceral leishmaniasis (cvl) presents problems related to its sensitivity and/or specificity. in the present study, a new leishmania-specific hypothetical protein, lihyd, was produced as a recombinant protein (rlihyd) and evaluated in elisa experiments for the cvl serodiagnosis. lihyd was characterized as antigenic in a recent immunoproteomic search performed with leishmania infantum proteins and the sera of dogs developing visceral leishmaniasis (vl). aiming to compa ...201626782811
development of a quantitative pcr for the detection of rangelia vitalii.the aim of this study was to develop and validate a sybr green qpcr assay to detect and quantify a fragment of the 18s rrna gene of rangelia vitalii in canine blood. repeatability of the qpcr was determined by the intra- and inter-assay variations. the qpcr showed efficiency of e=101.30 (r(2)=0.996), detecting as few as one copy of plasmid containing the target dna. specificity of the assay was performed using dna samples of babesia canis, b. gibsoni, ehrlichia canis, e. ewingii and leishmania s ...201626827871
development of recombinant bgp12 based enzyme linked immunosorbent assays for serodiagnosis of babesia gibsoni infection in dogs.indirect elisa and dot-elisa using recombinant bgp12 (rbgp12) were developed for the diagnosis of babesia gibsoni infected dogs. the complete open reading frame of bgp12 gene (378bp) was cloned in pet-32a(+) expression vector and expressed in escherichia coli as a soluble thioredoxin (trx) fusion protein. the purified rbgp12 was used for production of anti-rbgp12 rabbit serum, which recognized a native 12-kda protein in b. gibsoni infected erythrocyte by western blot analysis. to evaluate the po ...201626827835
molecular survey of anaplasma and ehrlichia of red deer and sika deer in gansu, china in 2013.anaplasma and ehrlichia are important emerging tick-borne pathogens in both humans and animals. here, we conducted a molecular surveillance study in gansu, china to assess the prevalence of anaplasma and ehrlichia spp. in red deer and sika deer based on polymerase chain reaction (pcr) analysis and sequencing of 16s rrna or msp genes. pcr revealed that the prevalence of anaplasma ovis, anaplasma bovis and anaplasma platys of the qilian mountain samples was 32%, 9% and 9%, respectively; the preval ...201625660960
clinical and laboratory features of canine anaplasma platys infection in 32 naturally infected dogs in the mediterranean basin.since the first description of anaplasma platys infection (api), the disease has been sporadically reported worldwide. whereas it is considered a subclinical disease in the united states or in australia, severe cases are reported in europe. thus far, little information is available regarding the clinical and laboratory findings associated with the disease and the implication of co-infections with other vector-borne pathogens (vbps) in southern europe. the purpose of the study was to describe cli ...201627426437
molecular characterization of ehrlichia canis infecting dogs, buenos aires.canine monocytic ehrlichiosis (cme) is a worldwide potentially fatal tick-borne rickettsial disease of dogs caused by ehrlichia canis and transmitted by rhipicephalus sanguineus sensu lato. cme diagnosis includes indirect (serology) and direct (e.g. blood smears and pcr) methods. pcr is more sensitive and specific than direct microscopic examination and positive pcr results confirm infection, whereas positive serologic test results only confirm exposure. the aim of the present study was to perfo ...201627236582
molecular survey of canine vector-borne diseases in stray dogs in thailand.despite the large population of stray dogs in thailand, there is limited information on the prevalence of canine vector-borne diseases (cvbds). in this study, a molecular survey was conducted to determine the prevalence of babesia spp., ehrlichia canis, hepatozoon spp., anaplasma platys and mycoplasma spp. in dogs in thailand. of the 181 dog blood samples tested by pcr, 78/181 (43.1%) were found to be infected with one or more pathogens. the overall prevalence rates of mycoplasma spp., hepatozoo ...201627143604
comparative experimental infection study in dogs with ehrlichia canis, e. chaffeensis, anaplasma platys and a. phagocytophilum.dogs acquire infections with the anaplasmataceae family pathogens, e. canis, e. chaffeensis, e. ewingii, a. platys and a. phagocytophilum mostly during summer months when ticks are actively feeding on animals. these pathogens are also identified as causing diseases in people. despite the long history of tick-borne diseases in dogs, much remains to be defined pertaining to the clinical and pathological outcomes of infections with these pathogens. in the current study, we performed experimental in ...201626840398
detection of ehrlichia canis, babesia vogeli, and toxoplasma gondii dna in the brain of dogs naturally infected with leishmania infantum.the aims of this study were to investigate the presence of leishmania infantum and possible co-infection with anaplasma platys , babesia vogeli, ehrlichia canis , and toxoplasma gondii in the brain of 24 dogs naturally infected by l. infantum . a total of 24 mongrel adult dogs (22 clinically affected, 2 with neurological signs, and 2 subclinically infected) aged between 2 and 5 yr, naturally infected by visceral leishmaniasis, were selected. fragments from meninges, frontal cortex, thalamus, cer ...201626765523
molecular identification and characterization of anaplasma platys and ehrlichia canis in dogs in mexico.the tick-borne pathogens ehrlichia canis and anaplasma platys are the causative agents of canine monocytic ehrlichiosis (cme) and canine cyclic thrombocytopenia (cct). although molecular evidence of e. canis has been shown, phylogenetic analysis of this pathogen has not been performed and a. platys has not been identified in mexico, where the tick vector rhipicephalus sanguineus sensu lato (s.l.) is common. the aim of this research was to screen, identify and characterize e. canis and a. platys ...201626615872
molecular detection of rickettsia conorii and other zoonotic spotted fever group rickettsiae in ticks, romania.the diverse tick fauna as well as the abundance of tick populations in romania represent potential risks for both human and animal health. spotted fever group (sfg) rickettsiae are recognized as important agents of emerging human tick-borne diseases worldwide. however, the epidemiology of rickettsial diseases has been poorly investigated in romania. in urban habitats, companion animals which are frequently exposed to tick infestation, play a role in maintenance of tick populations and as reservo ...201626507182
a survey of tick-borne pathogens in dogs and their ticks in the pantanal biome, brazil.tick and blood samples collected from domestic dogs in the brazilian pantanal were tested by molecular methods for the presence of tick-borne protozoa and bacteria. among 320 sampled dogs, 3.13% were infected by babesia vogeli (piroplasmida: babesiidae), 8.75% by hepatozoon canis (eucoccidiorida: hepatozoidae), 7.19% by anaplasma platys (rickettsiales: anaplasmataceae), and 0.94% by an unclassified anaplasma sp. in three tick species collected from dogs, the following tick-borne agents were dete ...201626467462
evaluation of gulf coast ticks (acari: ixodidae) for ehrlichia and anaplasma species.amblyomma maculatum koch (the gulf coast tick) is an aggressive, human-biting ixodid tick distributed throughout much of the southeastern united states and is the primary vector for rickettsia parkeri, an emerging human pathogen. amblyomma maculatum has diverse host preferences that include white-tailed deer, a known reservoir for ehrlichia and anaplasma species, including the human pathogens e. ewingii and e. chaffeensis to examine more closely the potential role of a. maculatum in the maintena ...201628031351
detection of mycoplasma haemocanis, mycoplasma haematoparvum, mycoplasma suis and other vector-borne pathogens in dogs from córdoba and santa fé, argentina.in argentina, only very few reports are available for canine tick-borne diseases where most are related to parasitic diseases. the objective of this survey was to investigate the prevalence of tick-borne pathogens in 70 dogs from santa fé and córdoba, argentina.201627978844
detection of 'candidatus neoehrlichia mikurensis' and other anaplasmataceae and rickettsiaceae in canidae in switzerland and mediterranean countries.'candidatus neoehrlichia mikurensis' is an emerging tick-borne zoonotic agent that primarily affects immunocompromised human patients. dogs and foxes are frequently exposed to ticks, and both species are in close proximity to humans. this is the first study to systematically investigate the occurrence of 'candidatus neoehrlichia mikurensis' in canidae in europa. we analyzed 1'739 blood samples from dogs in switzerland, italy, spain and portugal and 162 blood samples from free-ranging red foxes ( ...201627707682
molecular detection and identification of rickettsiales pathogens in dog ticks from costa rica.although vector-borne diseases are globally widespread with considerable impact on animal production and on public health, few reports document their presence in central america. this study focuses on the detection and molecular identification of species belonging to selected bacterial genera (ehrlichia, anaplasma and rickettsia) in ticks sampled from dogs in costa rica by targeting several genes: 16s rrna/dsb genes for ehrlichia; 16s rrna/groel genes for anaplasma, and ompa/glta/groel genes for ...201627480409
prevalence of vector-borne pathogens in dogs from haiti.canine vector-borne pathogens are common on some caribbean islands, but survey data in haiti are lacking. to determine the prevalence of selected vector-borne pathogens in dogs from haiti, we tested blood samples collected from 210 owned dogs, 28 (13.3%) of which were infested with rhipicephalus sanguineus ticks at the time of blood collection. no other tick species were identified on these dogs. a commercially available elisa identified antibodies to ehrlichia spp. in 69 (32.9%), antibodies to ...201627270383
molecular detection of anaplasma platys, ehrlichia canis, hepatozoon canis and rickettsia monacensis in dogs from maio island of cape verde archipelago.tick-borne diseases are emerging worldwide and have an important zoonotic relevance. dogs play an important role in the epidemiology of several zoonotic tick-borne pathogens acting as sentinels and/or reservoirs. this study focused on the molecular identification of tick-borne pathogens in blood samples of 153 autochthonous asymptomatic dogs in maio island, cape verde archipelago. eighty-four (54.9%) dogs were positive for one or more pathogens. fifty-five (35.9%) dogs were infected with hepatoz ...201627177475
molecular detection of zoonotic rickettsiae and anaplasma spp. in domestic dogs and their ectoparasites in bushbuckridge, south africa.members of the order rickettsiales are small, obligate intracellular bacteria that are vector-borne and can cause mild to fatal diseases in humans worldwide. there is little information on the zoonotic rickettsial pathogens that may be harbored by dogs from rural localities in south africa. to characterize rickettsial pathogens infecting dogs, we screened 141 blood samples, 103 ticks, and 43 fleas collected from domestic dogs in bushbuckridge municipality, mpumalanga province of south africa, be ...201626974185
serological and molecular investigation of ehrlichia spp. and anaplasma spp. in ticks and blood of dogs, in the thrace region of turkey.in recent years, tick-borne diseases like ehrlichiosis and anaplasmosis became widespread worldwide threatening the health of both human and companion animals. therefore, the aim of this study was to determine the presence of anaplasma spp., and ehrlichia spp. in dogs and ticks in the thrace region of turkey. a total of 400 blood samples and 912 ticks were collected from dogs living in shelters that are located in four cities (istanbul, edirne, tekirdag and kirklareli) of the thrace region. bloo ...201626961274
vector-borne diseases in stray dogs in peninsular malaysia and molecular detection of anaplasma and ehrlichia spp. from rhipicephalus sanguineus (acari: ixodidae) ticks.little data are available on the prevalence and transmission of vector-borne diseases in stray dogs in peninsular malaysia. this study was designed to determine the occurrence of vector-borne pathogens in malaysian stray dogs using serological and molecular approaches. in total, 48 dog blood samples were subjected to serological analysis using snap 4dx kit (idexx laboratories, westbrook, me). the presence of ehrlichia and anaplasma dna in the dog blood samples and rhipicephalus sanguineus (latre ...201626494821
parasites and vector-borne pathogens in client-owned dogs in albania. blood pathogens and seroprevalences of parasitic and other infectious agents.knowledge on the epidemiology of parasitic and vector-borne infections is still very limited for albania, a country located in the balkan peninsula in southeast europe. recent publications indicated prevalence rates of up to 52% for vector-borne infections in less-cared dogs in albania. to provide data on the epidemiological situation in dogs under veterinary care, a total of 602 client-owned dogs presented to four small animal clinics between march 2010 and april 2011 in tirana, albania, were s ...201626453093
molecular investigation of tick-borne pathogens in dogs from luanda, angola.no molecular data have been available on tick-borne pathogens that infect dogs from angola. the occurrence of agents from the genera anaplasma, babesia, ehrlichia and hepatozoon was assessed in 103 domestic dogs from luanda, by means of the polymerase chain reaction (pcr) and dna sequence analysis.201627160839
canine vector-borne pathogens in semi-domesticated dogs residing in northern cambodia.in southeast asia, the canine vector-borne pathogens babesia spp., ehrlichia canis, anaplasma platys, hepatozoon canis, haemotropic mycoplasmas and dirofilaria immitis cause significant morbidity and mortality in dogs. moreover, dogs have also been implicated as natural reservoirs for rickettsia felis, the agent of flea-borne spotted fever, increasingly implicated as a cause of undifferentiated fever in humans in southeast asia. the objective of this study was to determine the prevalence and div ...201627161452
retrospective analyses of dogs found serologically positive for ehrlichia canis in cebu, philippines from 2003 to 2014.the study aimed to document the clinical and hematological observations of dogs found serologically positive for ehrlichia canis and to identify parameters or factors that are associated with the disease with focus on the anemic and thrombocytopenic state of the infected dogs.201627051183
ehrlichiosis in household dogs and parasitized ticks in kerman-iran: preliminary zoonotic risk assessment.ehrlichiosis is an emerging tick-borne zoonotic disease caused by the family of anaplasmatacea. recently, outbreak of human monocytic ehrlichiosis was reported in northern part of iran. besides, serological evidence of canine monocytic ehrlichiosis caused by ehrlichia canis was reported from southeastern of iran but the epidemiology of this disease is almost undetermined in iran. the present study was designed to use pcr for detection of ehrlichia spp. in tick infested household dogs and determi ...201627308282
countrywide serological evaluation of canine prevalence for anaplasma spp., borrelia burgdorferi (sensu lato), dirofilaria immitis and ehrlichia canis in mexico.canine vector-borne diseases (cvbd) have become a major concern for canine and human public health. the aim of the study described here is to add epidemiological data regarding four pathogens responsible for cvbd, namely anaplasmosis, borreliosis, dirofilariosis and ehrlichiosis in a national survey conducted in mexico.201627474020
exposure to vector-borne pathogens in candidate blood donor and free-roaming dogs of northeast italy.many vector-borne pathogens including viruses, bacteria, protozoa and nematodes occur in northeast italy, representing a potential threat to animal and human populations. little information is available on the circulation of the above vector-borne pathogens in dogs. this work aims to (i) assess exposure to and circulation of pathogens transmitted to dogs in northeast italy by ticks, sandflies, and mosquitoes, and (ii) drive blood donor screening at the newly established canine blood bank of the ...201627357128
seroprevalence of vector-borne pathogens and molecular detection of borrelia afzelii in military dogs from portugal.canine vector-borne diseases (cvbds) are increasingly being reported worldwide and represent a serious threat to both animal and public health. military dogs may constitute a risk group for the agents causing these diseases, as they frequently work outdoors in different areas and are thus exposed to vector arthropods. in order to assess the risk of exposure of this type of dogs, a serological and molecular survey was conducted in military working dogs in portugal. one hundred apparently healthy ...201627160284
molecular detection of anaplasma spp. and ehrlichia spp. in ruminants from twelve provinces of china.anaplasma spp. and ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. there is little information on anaplasmosis and ehrlichiosis in ruminants in china. 16s rrna fret-qpcrs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of china for anaplasma spp. and ehrlichia spp. positive samples were further analyzed with a standard pcr for the glta. anaplasm ...201628096822
growth of ehrlichia canis, the causative agent of canine monocytic ehrlichiosis, in vector and non-vector ixodid tick cell lines.canine monocytic ehrlichiosis is caused by ehrlichia canis, a small gram-negative coccoid bacterium that infects circulating monocytes. the disease is transmitted by the brown dog tick rhipicephalus sanguineus s.l. and is acknowledged as an important infectious disease of dogs and other members of the family canidae worldwide. e. canis is routinely cultured in vitro in the canine monocyte-macrophage cell line dh82 and in non-vector ixodes scapularis tick cell lines, but not in cells derived from ...201626837859
comparative efficacy of oral administrated afoxolaner (nexgard™) and fluralaner (bravecto™) with topically applied permethrin/imidacloprid (advantix(®)) against transmission of ehrlichia canis by infected rhipicephalus sanguineus ticks to dogs.the ability of the topical spot-on advantix(®) (50 % permethrin/10 % imidacloprid) to prevent transmission of ehrlichia canis by infected rhipicephalus sanguineus ticks to dogs has previously been reported. the recent market introduction of chewable tablets containing the novel compounds, afoxolaner (nexgard™) and fluralaner (bravecto™) enabled us to conduct a comparative efficacy study with respect to the ability of these three products to block transmission of e. canis by ticks to dogs. the sp ...201627317101
detection of vector-borne pathogens in cats and their ectoparasites in southern italy.vector-borne pathogens are the subject of several investigations due to the zoonotic concern of some of them. however, limited data are available about the simultaneous presence of these pathogens in cats and their ectoparasites. the aim of the present study was to define the species of ectoparasites found on cats as well as to investigate vector-borne pathogens in cats and their ectoparasites in southern italy.201627160725
[photochemical studies on sulfanilamide, sulfacet-amide and 4-amino-benzoyl-ethylester]. 20165037303
membrane-bound ribosomes in hela cells. i. their proportion to total cell ribosomes and their association with messenger rna. 20164980477
intensive care nursing for renal patients. 20164950088
influence of patient age on forearm and systemic vascular response to hypoxaemia. 20165032887
the award of the trudeau medal for 1971 (andre f. cournand). 20164937533
[soviet literature on problems of medical parasitology and parasitic diseases for 1970]. 20164936200
a remarkable description of "aleppo boil" in 1839. 20164909855
evidence suggesting a sensory variation drive in humans. 20164862127
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