Publications

Influences of two antibiotic contaminants on the production, release and toxicity of microcystins.	The influences of spiramycin and amoxicillin on the algal growth, production and release of target microcystins (MCs), MC-LR, MC-RR and MC-YR, in Microcystis aeruginosa were investigated through the seven-day exposure test. Spiramycin were more toxic to M. aeruginosa than amoxicillin according to their 50 percent effective concentrations (EC(50)) in algal growth, which were 1.15 and 8.03µg/l, respectively. At environmentally relevant concentrations of 100ng/l-1µg/l, spiramycin reduced the total ...	2012	22074855
In vitro evaluation of antibacterial activity of Asparagopsis taxiformis from the Straits of Messina against pathogens relevant in aquaculture.	Ethanol extracts of Asparagopsis taxiformis collected from the Straits of Messina (Italy) were screened for antibacterial activity against pathogenic shellfish and fish bacteria previously isolated from local marine and brackish environments. Genetic labelling by DNA barcoding allowed us to identify the algal population as a biogeographical strain conspecific to A. taxiformis. The extract obtained in May showed the broadest antibacterial activity against all tested pathogenic bacteria, especiall ...	2012	22078751
The joint effects of sulfonamides and their potentiator on Photobacterium phosphoreum: differences between the acute and chronic mixture toxicity mechanisms.	Organisms are typically exposed to mixtures of chemicals over long periods of time; thus, chronic mixture toxicity analysis is the best way to perform risk assessment in regards to organisms. However, most studies focus on the acute mixture toxicity. To investigate the difference between chronic mixture toxicity and acute mixture toxicity, Photobacterium phosphoreum were exposed to chronic (24 h exposure) and acute (15 min exposure) toxicity of single sulfonamide (SA) and their potentiator (trim ...	2012	21944043
biodegradation of enteromorpha prolifera by mangrove degrading micro-community with physical-chemical pretreatment.	the bacteria involved in the biodegradation of enteromorpha prolifera (ep) are largely unknown, especially in offshore mangrove environments. in order to obtain the bacterial ep-degrading communities, sediments from a typical mangrove forest were sampled on the roots of mangrove in dongzhai port (haikou, china). the sediments were enriched with crude ep powders as the sole carbon source. the bacterial composition of the resulting mangrove-degrading micro-community (mdmc), named d2-1, was analyse ...	2011	21660543
synthesis and biological activity of novel n-cycloalkyl-(cycloalkylaryl)-2-[(3-r-2-oxo-2h-[1,2,4]triazino[2,3-c]quinazoline-6-yl)thio]acetamides.	in this paper the novel n-cycloalkyl-(cycloalkylaryl)-2-[(3-r-2-oxo-2h-[1,2,4]triazino[2,3-c]quinazoline-6-yl)thio]acetamides synthesis by aminolysis of activated by thionyl chloride or carbonyldiimidazole [(3-r-2-oxo-2h-[1,2,4]triazino[2,3-c]quinazolin-6-yl)-thio]acetic acids and alkylation of the 3-r-6-thio-6,7-dihydro-2h-[1,2,4]triazino[2,3-c]quinazoline-2-ones potassium salts with n-cycloalkyl-(cycloalkylaryl)-2-chloroacetamides are proposed. the structures of compounds are determined by (1) ...	2011	22051065
the effect of temperature on the bacterial load and microbial composition in norway lobster (nephrops norvegicus) tail meat during storage.	aims:ôçé the aim of this study was to update and extend our knowledge of the bacterial load and microbial composition in norway lobster (nephrops norvegicus) under commercially relevant storage conditions to optimize handling procedures. methods and results:ôçé total viable counts were performed at different storage temperatures (0, 4, 8, 10, 12 or 16-¦c) and after different storage times (1-7ôçâdays). storage at 16-¦c was found to be most detrimental, and storage at 0-¦c was found to be optimal ...	2011	21689222
historical microbiology: revival and phylogenetic analysis of the luminous bacterial cultures of m. w. beijerinck.	luminous bacteria isolated by martinus w. beijerinck were sealed in glass ampoules in 1924 and 1925 and stored under the names photobacterium phosphoreum and 'photobacterium splendidum'. to determine if the stored cultures were viable and to assess their evolutionary relationship with currently recognized bacteria, portions of the ampoule contents were inoculated into culture medium. growth and luminescence were evident after 13 days of incubation, indicating the presence of viable cells after m ...	2011	22066815
quantification of total and specific gram-negative histamine-producing bacteria species in fish using an mpn real-time pcr method.	quantification of histamine-producing bacteria (hpb) is necessary in order to elucidate the role that hpb play in scombrotoxin (histamine) fish poisoning. we report here the evaluation of a real-time pcr method for the quantification of total and specific gram-negative hpb species in fish using a most probable number (mpn) format. the species-specific real-time pcr assay was 100% inclusive for independently detecting morganella morganii, enterobacter aerogenes, raoultella planticola/ornithinolyt ...	2011	21839377
optimal conditions for stability of photoemission and freeze drying of two luminescent bacteria for use in a biosensor.	bioluminescent bacteria have been used for many years for biotoxicological analysis. one of the main concerns with this microorganism is the low experimental repeatability when subjected to external factors. the aim of the present study was to obtain accurate, sensitive, and repeatable measurements with stable signals (during the detection and over days) for application in a water-analysis device for the detection of pollutants. growth conditions were tested and optimized. an optimal freeze-dryi ...	2011	21191881
salmonella typhimurium st213 is associated with two types of inca/c plasmids carrying multiple resistance determinants.	salmonella typhimurium st213 was first detected in the mexican typhimurium population in 2001. it is associated with a multi-drug resistance phenotype and a plasmid-borne blacmy-2 gene conferring resistance to extended-spectrum cephalosporins. the objective of the current study was to examine the association between the st213 genotype and blacmy-2 plasmids.	2011	21223599
a cmp-n-acetylneuraminic acid synthetase purified from a marine bacterium, photobacterium leiognathi jt-shiz-145.	a cytidine 5'-monophospho-n-acetylneuraminic acid (cmp-neu5ac) synthetase was found in a crude extract prepared from photobacterium leiognathi jt-shiz-145, a marine bacterium that also produces a ß-galactoside a2,6-sialyltransferase. the cmp-neu5ac synthetase was purified from the crude extract of the cells by a combination of anion-exchange and gel filtration column chromatography. the purified enzyme migrated as a single band (60 kda) on sodium dodecylsulfate-polyacrylamide gel electrophoresis ...	2011	21228490
transesterification using the cross-linked enzyme aggregate of photobacterium lipolyticum lipase m37.	biodiesel is methyl and ethyl esters of long-chain fatty acids produced from vegetable oils or animal fats. lipase enzymes have occasionally been used for the production of this biofuel. recently, biodiesel production using immobilized lipase has received increased attention. through enhanced stability and reusability, immobilized lipase can contribute to the reduction of the costs inherent to biodiesel production. in this study, methanol-tolerant lipase m37 from photobacterium lipolyticum was i ...	2011	22127127
bioavailability, ecotoxicity, and geological characteristics of trace lead in sediments from two sites on negro river, uruguay, south america.	bioassays of two sites along the rio negro in uruguay indicate ecotoxicity, which could be attributable to trace concentrations of lead in river sediments. monthly samples at two sites at baygorria and bonete locations were analyzed for both particle size and lead. lead was determined by atomic spectrometry in river water and sediment and particle size by sieving and sedimentation. data showed that baygorria's sediments have greater percentage of clay than bonete's (20.4 and 5.8%, respectively). ...	2011	21968803
effect of americium-241 on luminous bacteria. role of peroxides.	the effect of americium-241 ((241)am), an alpha-emitting radionuclide of high specific activity, on luminous bacteria photobacterium phosphoreum was studied. traces of (241)am in nutrient media (0.16-6.67 kbq/l) suppressed the growth of bacteria, but enhanced luminescence intensity and quantum yield at room temperature. lower temperature (4 -¦c) increased the time of bacterial luminescence and revealed a stage of bioluminescence inhibition after 150 h of bioluminescence registration start. the r ...	2011	21388726
identification and cloning of a selenophosphate synthetase (sps) from tiger shrimp, penaeus monodon, and its transcription in relation to molt stages and following pathogen infection.	complementary (c)dna encoding selenophosphate synthetase (sps) messenger (m)rna of the tiger shrimp penaeus monodon, designated pmsps, was obtained from the hepatopancreas by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). the 1582-bp cdna contained an open reading frame (orf) of 1248bp, a 103-bp 5'-untranslated region (utr), and a 231-bp 3'-utr, which contained a conserved selenocysteine insertion sequence (secis) element, a conventional p ...	2011	21664929
fluorescence-pcr assays and isolation of luminescent bacterial clones using an automated plate reader.	the genes responsible for luminescence in various species of the marine microorganism photobacterium, have been used for many years as a tool by researchers and instructors. in particular, the lux operon of photobacterium fischeri has been used by many instructors to teach recombinant dna techniques. two methods using an automated plate reader and multiwell plates were applied to a set of previously-published exercises. in these exercises that involve transfer of lux genes to escherichia coli to ...	2011	21445904
favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, shewanella violacea dss12, at high-hydrostatic pressures.	shewanella violacea dss12, a deep-sea bacterium, produces eicosapentaenoic acid (epa) as a component of membrane phospholipids. although various isolates from the deep sea, such as photobacterium profundum ss9, colwellia psychrerythraea 34h and various shewanella strains, produce epa- or docosahexaenoic acid-containing phospholipids, the physiological role of these polyunsaturated fatty acids remains unclear. in this article, we illustrate the physiological importance of epa for high-pressure ad ...	2011	21518217
treatment of a textile effluent from dyeing with cochineal extracts using trametes versicolor fungus.	trametes versicolor (tv) fungus can degrade synthetic dyes that contain azo groups, anthraquinone, triphenylmethane polymers, and heterocyclic groups. however, no references have been found related to the degradation of natural dyes, such as the carminic acid that is contained in the cochineal extract. experiments to determine the decolorization of the effluent used in the cotton dyeing process with cochineal extract by means of tv fungus were done. treatments to determine decolorization in the ...	2011	21552764
Atomic charges of individual reactive chemicals in binary mixtures determine their joint effects: An example of cyanogenic toxicants and aldehydes.	Environmental contaminants are usually encountered as mixtures, and many of these mixtures yield synergistic or antagonistic effects attributable to an intracellular chemical reaction that pose a potential threat on ecological systems. However, how atomic charges of individual chemicals determine their intracellular chemical reactions, and then determine the joint effects for mixtures containing reactive toxicants, is not well understood. To address this issue, the joint effects between cyanogen ...	2011	22105991
culture-based and denaturing gradient gel electrophoresis analysis of the bacterial community structure from the intestinal tracts of earthworms(eisenia fetida).	the bacterial communities in the intestinal tracts of earthworm were investigated by culture-dependent and - independent approaches. in total, 72 and 55 pure cultures were isolated from the intestinal tracts of earthworms under aerobic and anaerobic conditions, respectively. aerobic bacteria were classified as aeromonas (40%), bacillus (37%), photobacterium (10%), pseudomonas (7%), and shewanella (6%). anaerobic bacteria were classified as aeromonas (52%), bacillus (27%), shewanella (12%), paeni ...	2011	21952364
Molecular mechanisms of hepcidin regulation in sea bass (Dicentrarchus labrax).	Hepcidin, an antimicrobial peptide described as a key regulator of iron metabolism, is known to respond in mammals to several stimuli, including iron overload, anemia, hypoxia and inflammation, through a number of molecular pathways. In order to understand the molecular pathways involved in the regulation of hepcidin expression in teleost fish, we have isolated for European sea bass (Dicentrarchus labrax) several coding sequences of known molecules involved on these pathways in mammals, namely j ...	2011	22019826
refined identification of vibrio bacterial flora from acanthasther planci based on biochemical profiling and analysis of housekeeping genes.	we used a polyphasic approach for precise identification of bacterial flora (vibrionaceae) isolated from crown-of-thorns starfish (cots) from lizard island (great barrier reef, australia) and guam (u.s.a., western pacific ocean). previous 16s rrna gene phylogenetic analysis was useful to allocate and identify isolates within the photobacterium, splendidus and harveyi clades but failed in the identification of vibrio harveyi-like isolates. species of the v harveyi group have almost indistinguisha ...	2011	22013751
acute toxicity and n-octanol/water partition coefficients of substituted thiophenols: determination and qsar analysis.	the acute toxicity (-logec(50)) to photobacterium phosphoreum and the n-octanol/water partition coefficient (logk(ow)) of 31 kinds of substituted thiophenols were determined at 298.15k. the -logec(50) values of studied chemicals are between 4.26 and 5.89. their logk(ow) values are between 1.34 and 4.02. comparative molecular field (comfa) and comparative molecular similarity index analysis (comsia) models established were successful in predicting -logec(50) and logk(ow) values of halogenated, me ...	2011	22154146
bacterial community associated with the intestinal tract of p. monodon in commercial farms.	the potentially important roles of intestinal bacteria on immune response, disease resistance, and nutrition for the black tiger shrimp penaeus monodon have been increasingly investigated. however, so far, little is known about the intestinal bacterial community of the shrimp in the commercial aquaculture settings. in this study, the intestinal bacterial communities of juvenile p. monodon (70 individuals) from eight commercial farms in thailand were examined using 16s rdna pcr-dgge, and seven 16 ...	2011	21915632
photobacterium atrarenae sp. nov. a novel bacterium isolated from sea sand.	the gram-reaction-negative, motile, facultatively anaerobic, catalase-positive, oxidase-positive bacterial strain m3-4(t) was isolated from black sea sand and subjected to a taxonomic study. cells of strain m3-4(t) have monotrichous flagella, grow optimally at 37°c and at ph 7-8 in the presence of 1-4% (w/v) nacl and hydrolyze casein, starch and l: -tyrosine. according to phylogenetic analyses using 16s rrna gene sequences, strain m3-4(t) belongs to the genus photobacterium and is most closely r ...	2011	21861148
Reduction of diazinon and dimethoate in apple juice by pulsed electric field treatment.	BACKGROUND: Organophosphorus pesticides (OPPs) are widely used in agricultural production in China, and residues of OPPs in agro-products and foods have become a public health concern. Chronic exposure to OPPs can result in potential immunosuppressive effects, cytotoxicity and mutagenicity. Pulsed electric fields (PEFs) have the potential to be used as an alternative to conventional techniques of food production. The aim of the present study was to investigate the influence of PEFs on the degrad ...	2011	21919007
Acute Toxicity of Alkylpolyglucosides to Vibrio fischeri, Daphnia magna and Microalgae: A Comparative Study.	In this paper, toxicity values of alkylpolyglucosides have been determined by applying the 24-h immobilization test with Daphnia magna, the LumiStox(®) 300 test which employs the luminescent bacteria Photobacterium phosphoreum and the test with Selenastrum capricornutum. Three alkylpolyglucosides with different alkyl chain and degree of polymerisation have been tested. For all tests, the results indicated that Vibrio fischeri was more sensitive to toxic effects from alkylpolyglucosides than was ...	2011	22127434
evolution of inca/c blacmy-2-carrying plasmids by acquisition of the blandm-1 carbapenemase gene.	the bla(ndm-1) gene has been reported to be often located on broad-host-range plasmids of the inca/c type in clinical but also environmental bacteria recovered from the new delhi, india, area. inca/c-type plasmids are the main vehicles for the spread of the cephalosporinase gene bla(cmy-2), frequently identified in the united states, canada, and europe. in this study, we completed the sequence of inca/c plasmid pndm-kn carrying the bla(ndm-1) gene, recovered from a klebsiella pneumoniae isolate ...	2011	22123704
Bacterial bioluminescence as a lure for marine zooplankton and fish.	The benefits of bioluminescence for nonsymbiotic marine bacteria have not been elucidated fully. One of the most commonly cited explanations, proposed more than 30 y ago, is that bioluminescence augments the propagation and dispersal of bacteria by attracting fish to consume the luminous material. This hypothesis, based mostly on the prevalence of luminous bacteria in fish guts, has not been tested experimentally. Here we show that zooplankton that contacts and feeds on the luminescent bacterium ...	2011	22203999
photobacterium swingsii sp. nov., isolated from marine organisms.	six gram-negative coccobacilli were isolated from pacific oysters (crassostrea gigas) from mexico and haemolymph of spider crabs (maja brachydactyla) from spain. all of the isolates grew as small green colonies on thiosulphate-citrate-bile salts-sucrose (tcbs) agar and were facultatively anaerobic, oxidase-positive and sensitive to the vibriostatic agent o/129. repetitive palindromic pcr analysis revealed a high degree of genomic homogeneity among the isolates. several phenotypic traits differen ...	2011	20228205
evidence for the role of horizontal transfer in generating pvt1, a large mosaic conjugative plasmid from the clam pathogen, vibrio tapetis.	the marine bacterium vibrio tapetis is the causative agent of the brown ring disease, which affects the clam ruditapes philippinarum and causes heavy economic losses in north of europe and in eastern asia. further characterization of v. tapetis isolates showed that all the investigated strains harbored at least one large plasmid. we determined the sequence of the 82,266 bp plasmid pvt1 from the cect4600(t) reference strain and analyzed its genetic content. pvt1 is a mosaic plasmid closely relate ...	2011	21326607
effect of halogenated fluorescent compounds on bioluminescent reactions.	the paper investigates an application of luminescent bioassays to monitor the toxicity of organic halides. effects of xanthene dyes (fluorescein, eosin y, and erythrosin b), used as model compounds, on bioluminescent reactions of firefly luciola mingrelica, marine bacteria photobacterium leiognathi, and hydroid polyp obelia longissima were studied. dependence of bioluminescence quenching constants on the atomic weight of halogen substituents in dye molecules was demonstrated. bacterial biolumine ...	2011	21336798
effect of enzyme producing microorganisms on the biomass of epigeic earthworms (eisenia fetida) in vermicompost.	we analyzed the bacterial community structure of the intestines of earthworms and determined the effect of enzyme producing microorganisms on the biomass of earthworms in vermicompost. fifty-seven bacterial 16s rdna clones were identified in the intestines of earthworms by using polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) analysis. entomoplasma somnilux and bacillus licheniformis were the dominant microorganisms; other strains included aeromonas, bacillus, clostr ...	2011	21421302
genome sequence of photobacterium mandapamensis strain svers.1.1, the bioluminescent symbiont of the cardinal fish siphamia versicolor.	photobacterium mandapamensis is one of three luminous photobacterium species able to form species-specific bioluminescent symbioses with marine fishes. here, we present the draft genome sequence of p. mandapamensis strain svers.1.1, the bioluminescent symbiont of the cardinal fish siphamia versicolor, the first genome of a symbiotic, luminous photobacterium species to be sequenced. analysis of the sequence provides insight into differences between p. mandapamensis and other luminous and symbioti ...	2011	21478348
molecular and functional characterization of the gilthead seabream β-defensin demonstrate its chemotactic and antimicrobial activity.	antimicrobial peptides (amps) are important mediators of the innate immune response against bacteria and viruses. we have found a β-defensin (bd) gene searching the expressed sequence tags (ests) of the teleost fish gilthead seabream (sparus aurata). the clone contains an open reading frame of 201bp mrna that encodes a putative seabream β-defensin (sabd) propeptide of 66 amino acids containing the six conserved cysteines as the main signature of this amp. the phylogenetic tree shows that sabd, a ...	2011	21497909
natural history of slc11 genes in vertebrates: tales from the fish world.	abstract:	2011	21501491
occurrence of both subspecies of photobacterium damselae in mullets collected in the river magra (italy).	the natural reservoirs and biological characteristics of pathogenic populations of both subspecies of photobacterium damselae in aquatic habitants remain unclear because of difficulties in obtaining pathogenic strains from the environment. in the present study, we assessed the occurrence of photobacterium damselae subsp. piscicida and photobacterium damselae subsp. damselae , considered to be the causative agent of past epizootic outbreaks in mullets collected in the river magra, italy. two hund ...	2011	21529120
susceptibility to antibiotics of vibrio spp. and photobacterium damsela ssp. piscicida strains isolated from italian aquaculture farms.	the antibiotic resistance patterns of aetiological agents responsible for vibriosis and pasteurellosis were studied to contribute to control the spread of these two bacterial diseases in mediterranean fish farming. strains of photobacterium damsela ssp. piscicida, vibrio fluvialis, vibrio alginolyticus, vibrio parahaemolyticus, vibrio metschnikovii, isolated from italian aquaculture (fish, shellfish and crustaceans) sites, were assayed for their susceptibility to some antibacterial agents curren ...	2011	21344147
seafood quality analysis: molecular identification of dominant microbiota after ice storage on several general growth media.	this study points out the limitations of several general growth media frequently used in seafood research by a systematic identification of the microorganisms on fish samples during ice storage unable to grow on those media. aerobic psychrotrophic count (apc), replication on various general media and total cultivable microbial community denaturing gradient gel electrophoresis (dgge) analysis revealed that many potential spoilage microorganisms were overlooked. those microorganisms overlooked by ...	2011	21645815
phylogenetic analysis of culturable marine bacteria in sediments from the south korean yellow sea.	biogeochemical and microbiological characterization of marine sediments taken from the yellow sea of south korea was carried out. one hundred and thirty six bacterial strains were isolated, characterized and phylogenetic relationship was evaluated. the gene sequences of 16s rdna regions were examined to study the phylogenetic analysis of bacterial community in the marine sediments. among 136 isolates, 5 bacterial isolates were identified as novel members, remaining 131 isolates were fall into 5 ...	2011	21675219
[glycosides of hydroxylamine derivatives. i. phase transfer synthesis and study of isatine-3-oximes glucosaminides influence on bacterial luminescence].	in the phase transfer system solid calcium carbonate-acetonitrile, per acetate alpha-d-glucosaminilchloride glycosilate easily deprotoned isatine-3-oximes hydroxyl groups. it was found that the presence in the reaction mixture a catalytic amounts of 15-crown-5 accelerated the process twice. obtained o-beta-d-glucosaminides were identified with 1h-nmr spectroscopy. features of synthesized compound's nmr spectra are discussed in comparison with those of another n-acetylglucosamine 1-o-derivatives. ...	2011	21721259
efficient chemoenzymatic synthesis of sialyl tn-antigens and derivatives.	an n-terminal and c-terminal truncated recombinant +¦2-6-sialyltransferase cloned from photobacterium sp. jh-ish-224, psp2,6st(15-501)-his(6), was shown to be an efficient catalyst for one-pot three-enzyme synthesis of sialyl tn (stn) antigens and derivatives containing natural and non-natural sialic acid forms.	2011	21725542
microarray based analysis of inca/c plasmid associated genes from multidrug resistant salmonella enterica.	in enterobacteriaceae, plasmids have been classified according to 27 incompatibility (inc) or replicon types which are based on the inability of different plasmids with the same replication mechanism to co-exist in the same cell. certain replicon types such as inca/c are associated with multidrug resistance (mdr). we developed a microarray that contains 286 unique 70 mer oligonucleotide probes based on sequences from five inca/c plasmids: pyr1 (yersinia ruckeri), ppip1202 (yersinia pestis), pp99 ...	2011	21841024
Hydrophobicity-dependent QSARs to predict the toxicity of perfluorinated carboxylic acids and their mixtures.	Perfluorinated carboxylic acids (PFCAs) have wide industrial applications because of their unique physicochemical characteristics. However, data on the toxicity of much of this chemical class is lacking, particularly with regard to mixture toxicity. In this study, the toxicity of individual PFCAs and their mixtures to Photobacterium phosphoreum were observed. There was a tendency of increasing toxicity from C3 to C14 PFCA and a tendency of decreasing toxicity from C14 to C18 PFCA because of "the ...	2011	21843807
purification of a toxic metalloprotease produced by the pathogenic photobacterium damselae subsp. piscicida isolated from cobia (rachycentron canadum).	the aim of the present study was to purify and characterize a toxic protease secreted by the pathogenic photobacterium damselae subsp. piscicida strain cp1 originally isolated from diseased cobia (rachycentron canadum). the toxin isolated by anion exchange chromatography, was a metalloprotease, inhibited by l-cysteine, ethylenediaminetetraacetic acid (edta), ethylene glycol-bis(beta-aminoethyl ether)n,n,n',n'-tetraacetic acid (egta), 1,10-phenanthroline, n-tosyl-l-phenylalanine-chloromethyl keto ...	2011	21812347
dietary arginine and repeated handling increase disease resistance and modulate innate immune mechanisms of senegalese sole (solea senegalensis kaup, 1858).	stress is known to impair immune function and disease resistance in fish. in the present study, repeated handling was employed as a chronic stressor in order to verify whether its attributed immunosuppressive effects could be minimized by dietary arginine supplementation. therefore, senegalese sole (solea senegalensis) were air exposed daily for 3-ámin during 14-ádays (handling) or left undisturbed (control). in-áaddition, both control and handled specimens were fed 3 diets with graded levels of ...	2011	21820517
control of halophilic histamine-producing bacteria by using natural antimicrobials and other agents.	we have investigated the control of halophilic histamine-producing bacteria, photobacterium phosphoerum and photobacterium damselae by using vapor of essential oils and other agents. three of fourteen essential oils showed high antibacterial activity against histamine-producing bacteria. among food additives and detergents, ten of twenty-one food additives and seven of nine detergents also showed antibacterial activity. low concentrations of sodium chloride suppressed the growth of both species. ...	2011	22200797
The Photobacterium damselae subsp. damselae hemolysins damselysin and HlyA are encoded within a new virulence plasmid.	Photobacterium damselae subsp. damselae (formerly Vibrio damsela) is a marine bacterium that causes infections and fatal disease in a wide range of marine animals and in humans. Highly hemolytic strains produce damselysin (Dly), a cytolysin encoded by the dly gene that is lethal for mice and has hemolytic activity. We found that Dly is encoded in the highly hemolytic strain RM-71 within a 153,429-bp conjugative plasmid that we dubbed pPHDD1. In addition to Dly, pPHDD1 also encodes a homologue of ...	2011	21875966
Study of the microbial ecology of wild and aquacultured Tunisian fresh fish.	Eighty samples of fresh fish were collected in Tunisia and analyzed for microbial load. Quality and hygienic safety of the meat and intestines of wild and aquacultured fresh fish were determined. The mesophilic aerobic plate count and populations of psychrotrophic lactic acid bacteria (LAB) and other psychrotrophic bacteria ranged from 5.67 to 7.29, 4.51 to 6, and 5.07 to 6.21 log CFU/g, respectively. For all microbiological determinations, bacterial counts were lower in meat than in the intest ...	2011	22004828
a pilot study of the water quality of the yarra river, victoria, australia, using in vitro techniques.	a pilot study was initiated to provide the first information on the recombinant receptor-reporter gene bioassay (hormonal) activity of freshwaters in victoria. the project involved the collection of water samples from six stations on the main stem of the yarra river in and upstream of the city of melbourne, australia in april 2008 and april 2009. samples were prepared for measurement of sample toxicity using a modified photobacterium test, genotoxicity using a high-throughput luminescent umu tes ...	2011	21894495
characterisation of the microbiota of atlantic mackerel (scomber scombrus).	in this study the microbiota of atlantic mackerel (scomber scombrus) collected by a commercial purse seiner was examined. fish were collected directly from the purse seine and from the refrigerated sea water (rsw) transport tank after loading. the culturable microbiota and specific spoilage bacteria (ssb) were quantified on iron agar lyngby (ial) and identified using commercially available biochemical api® kits on pure cultured isolates. these kits showed to be sub-optimal in characterising the ...	2011	21914558
effect of humic substances on toxicity of inorganic oxidizer bioluminescent monitoring.	the current study deals with the effect of humic substances (hs) on toxicity of solutions of a model inorganic oxidizer, potassium ferricyanide. chemical reactions responsible for toxicity changes are under consideration. the bioluminescent system of coupled enzymatic reactions catalyzed by bacterial luciferase and oxidoreductase was used as a bioassay. general and oxidative toxicity of ferricyanide solutions were evaluated. ability of hs to decrease or increase general and oxidative toxicity of ...	2011	21309025
in vitro study of possible microbial indicators for drowning: salinity and types of bacterioplankton proliferating in blood.	numbers and types of bacterioplankton proliferating in blood samples mixed with water of various salinity levels were examined to determine the characteristics of species associated with salinity. water samples (total n=88) were collected from the midstream of two rivers (freshwater; n=10; salinity <0.05%), from around their estuaries (areas of freshwater, n=20, salinity <0.05%; areas of brackish water, n=20, salinity <0.05-3.1%; areas of marine water beyond the mouths of the rivers, n=28, salin ...	2011	20554407
enhanced nitrogen removal from pharmaceutical wastewater using sba-anammox process.	efficient biological nitrogen removal from pharmaceutical wastewater has been focused recently. the present study dealt with the treatment of colistin sulfate and kitasamycin manufacturing wastewater through anaerobic ammonium oxidation (anammox). the biotoxicity assay on luminescent bacterium photobacterium phosphoreum (t3 mutation) showed that the pharmaceutical wastewater imparted severe toxicity with a relative luminosity of 3.46% ± 0.45%. during long-term operation, the cumulative toxicity ...	2011	20832098
changes in complement responses in gilthead seabream (sparus aurata) and european seabass (dicentrarchus labrax) under crowding stress, plus viral and bacterial challenges.	gilthead seabream (sparus aurata) and european seabass (dicentrarchus labrax) were subjected to either experimental infection with photobacterium damselae subsp. piscicida or nodavirus after a period of 2 weeks of crowding in which fish were subjected to a 5-fold increase in density (10-50 kg/m(3)). samples were obtained before the crowding period (0 h or control) and at 24h and 72 h after crowding from both groups of infected fish. the complement haemolytic activity and the expression of the c3 ...	2010	20951810
the bacterial exotoxin aip56 induces fish macrophage and neutrophil apoptosis using mechanisms of the extrinsic and intrinsic pathways.	it has been previously shown that the exotoxin of the important fish pathogen photobacterium damselae ssp. piscicida is a key pathogenicity factor and is responsible for the extensive systemic apoptosis of macrophages and neutrophils seen in acute fish photobacteriosis. the focus of the present study was to further characterize the aip56-induced apoptosis of sea bass professional phagocytes by assessing the involvement of caspases, mitochondria and oxidative stress. the resulting data indicate t ...	2010	20969963
enzymatic synthesis and properties of glycoconjugates with legionaminic acid as a replacement for neuraminic acid.	in addition to sialic acid, bacteria produce several other nonulosonic acids, including legionaminic acid (leg). this has exactly the same stereochemistry as sialic acid, with the added features of 9-deoxy and 7-amino groups. in order to explore the biological effects of replacing sialic acid residues (neu5ac) in glycoconjugates with leg in its diacetylated form, diacetyllegionaminic acid (leg5ac7ac), we tested cmp-leg5ac7ac as a donor substrate with a selection of bacterial and mammalian sialyl ...	2010	20978010
total and pathogenic vibrio parahaemolyticus in shrimp: fast and reliable quantification by real-time pcr.	vibrio parahaemolyticus is found in aquatic environments and is the leading cause of gastroenteritis due to seafood consumption worldwide. we evaluated a quantitative real-time pcr (q-pcr) assay with hydrolysis probes, to determine whether this method could be used for the efficient counting of total, tdh and trh-positive v. parahaemolyticus in shrimps. we assessed the specificity of this assay, using 62 strains from 12 non target bacterial species of the vibrio, photobacterium, shewanella and a ...	2010	20843573
spoilage-related microbiota associated with chilled beef stored in air or vacuum pack.	in order to study the spoilage-related microbiota of beef at species level, a combination of culture-independent and culture-dependent methods was used to analyse nine different beef samples stored at 4°c in air or in vacuum pack. plate counts on selective agars after 0, 7 and 20 days of storage showed that vacuum packaging reduced the viable counts of brochothrix thermosphacta, pseudomonas spp. and enterobacteriaceae, whereas the growth of lactic acid bacteria (lab) was unaffected. storage in v ...	2010	21056779
resurgence of morbillivirus infection in mediterranean dolphins off the french coast.		2010	20495168
molecular cloning of sea bass (dicentrarchus labrax l.) caspase-8 gene and its involvement in photobacterium damselae ssp. piscicida triggered apoptosis.	caspase-8 is an initiator caspase that plays a crucial role in some cases of apoptosis by extrinsic and intrinsic pathways. caspase-8 structure and function have been extensively studied in mammals, but in fish the characterization of that initiator caspase is still scarce. in this work, the sea bass counterpart of mammalian caspase-8 was sequenced and characterized, and its involvement in the apoptogenic activity of a toxin from a fish pathogen was assessed. a 2472 bp cdna of sea bass caspase-8 ...	2010	20202478
acute toxicity evaluation of explosive wastewater by bacterial bioluminescence assays using a freshwater luminescent bacterium, vibrio qinghaiensis sp. nov.	the compositions of explosive wastewater generated from tnt (2,4,6-trinitrotoluene) purification stage were characterized by using uv-vis spectroscopy, fourier transform infrared spectroscopy (ftir), high performance liquid chromatograph (hplc) and gas chromatograph/mass spectroscopy (gc/ms). the acute toxicity was evaluated by bacterium bioluminescence assay using a freshwater luminescent bacterium (vibrio qinghaiensis sp. nov.) and a marine luminescent bacterium (photobacterium phosphoreum). t ...	2010	21185645
biodegradable gelatin-chitosan films incorporated with essential oils as antimicrobial agents for fish preservation.	essential oils of clove (syzygium aromaticum l.), fennel (foeniculum vulgare miller), cypress (cupressus sempervirens l.), lavender (lavandula angustifolia), thyme (thymus vulgaris l.), herb-of-the-cross (verbena officinalis l.), pine (pinus sylvestris) and rosemary (rosmarinus officinalis) were tested for their antimicrobial activity on 18 genera of bacteria, which included some important food pathogen and spoilage bacteria. clove essential oil showed the highest inhibitory effect, followed by ...	2010	20688230
photobacterium gaetbulicola sp. nov., a lipolytic bacterium isolated from a tidal flat sediment.	a gram-negative, motile, non-spore-forming and lipolytic bacterial strain, designated gung47(t), was isolated from a tidal flat on the west coast of korea. strain gung47(t) grew optimally at 30 °c and with 2-5 % (w/v) nacl. phylogenetic analyses based on 16s rrna gene sequences revealed that strain gung47(t) belonged to the genus photobacterium. strain gung47(t) exhibited 98.1 % 16s rrna gene sequence similarity with photobacterium rosenbergii lmg 22223(t) and 94.3-96.3 % similarity with other t ...	2010	20023056
properties of tryptophan indole-lyase from a piezophilic bacterium, photobacterium profundum ss9.	tryptophan indole-lyase (trpase), pbpra2532, from photobacterium profundum ss9, a piezophilic marine bacterium, has been cloned, expressed in escherichia coli, and purified. the p. profundum trpase (pptrpase) exhibits similar substrate specificity as the enzyme from e. coli (ectrpase). pptrpase has an optimum temperature for activity at about 30°c, compared with 53°c for ectrpase, and loses activity rapidly (t(1/2)∼30min) when incubated at 50°c, while ectrpase is stable up to 65°c. pptrpase reta ...	2010	21081107
cloning and characterization of dihydrofolate reductases from deep-sea bacteria.	enzymes from organisms living in deep-sea are thought to have characteristic pressure-adaptation mechanisms in structure and function. to better understand these mechanisms in dihydrofolate reductase (dhfr), an essential enzyme in living cells, we cloned, overexpressed and purified four new dhfrs from the deep-sea bacteria shewanella violacea (svdhfr), photobacterium profundum (ppdhfr), moritella yayanosii (mydhfr) and moritella japonica (mjdhfr), and compared their structure and function with t ...	2010	20040594
elucidation of exo-beta-d-glucosaminidase activity of a family 9 glycoside hydrolase (pbpra0520) from photobacterium profundum ss9.	a glycoside hydrolase (gh) gene from photobacterium profundum ss9 (pbpra0520) belonging to gh family 9 was expressed in escherichia coli. the protein was expressed with the intact n-terminal sequence, suggesting that it is an intracellular enzyme. the recombinant protein showed hydrolytic activity toward chitobiose [(glcn)(2)] and cellobiose (cg(2)) in various disaccharides. this protein also released 4-nitrophenol (pnp) from both 4-nitrophenyl-β-d-glucosaminide (glcn-pnp) and 4-nitrophenyl-β-d- ...	2010	21098515
mixture toxicity from photosystem ii inhibitors on microalgal community succession is predictable by concentration addition.	the typical pollution situation involves chemical mixtures, and assessing the risks of single chemicals one at a time is not sufficient. concentration addition (ca) has been suggested as a predictive tool in mixture ecotoxicology. the accuracy of ca for mixtures of similarly acting chemicals has been demonstrated under relatively simple biological conditions in single-species tests. to consider the high diversity of interconnected species in ecosystems, one must evaluate ca on a community level ...	2010	20836067
toxicity prediction of antibiotics on luminescent bacteria, photobacterium phosphoreum, based on their quantitative structure-activity relationship models.	acute toxicity (ec₅₀₋₃₀ min) and chronic toxicity (ec₅₀₋₂₄ h) of 21 antibiotics on photobacterium phosphoreum was observed and quantitative structure-activity relationship (qsar) models were developed, respectively. by comparing these two qsar models, the following model was established, log(1/ec₅₀₋₂₄ h) = 1.8283 + 1.1503log(1/ec ₅₀₋₃₀ min) + 0.2872elomo - 0.0901dm + 0.0003pmiz + 0.0088psa - 0.0382sd, r² = 0.8513, where elomo is lowest occupied molecular energy, dm is dipole moment, pmiz is prin ...	2010	21107523
rapid determination of the toxicity of quantum dots with luminous bacteria.	in this paper, a novel method so-called bioluminescence inhibition assay with luminous bacteria (photobacterium phosphoreum) was introduced to evaluate the toxicity of quantum dots. the bioassay was based on measuring the decrease of the light emitted by luminous bacteria. with obvious advantages of simplicity, rapidity and sensitivity, it can dramatically improve the efficiency of probing the toxicity of qds. based on this method, we systemically explored the effect of the composition and surfa ...	2010	20056317
rapid identification and characterization of vibrio species using whole-cell maldi-tof mass spectrometry.	vibrio identification by means of traditional microbiological methods is time consuming because of the many biochemical tests that have to be performed to distinguish closely related species. this work aimed at evaluating the use of maldi-tof mass spectrometry for the rapid identification of vibrio (v.) spp. as an advantageous application to rapidly discriminate the most important vibrio spp. and distinguish vibrio spp. from closely related bacterial species like photobacterium damselae and grim ...	2010	20059616
marine bacterial sialyltransferases.	sialyltransferases transfer n-acetylneuraminic acid (neu5ac) from the common donor substrate of these enzymes, cytidine 5'-monophospho-n-acetylneuraminic acid (cmp-neu5ac), to acceptor substrates. the enzymatic reaction products including sialyl-glycoproteins, sialyl-glycolipids and sialyl-oligosaccharides are important molecules in various biological and physiological processes, such as cell-cell recognition, cancer metastasis, and virus infection. thus, sialyltransferases are thought to be imp ...	2010	21139844
toxicity of photobacterium damselae subsp. damselae strains isolated from new cultured marine fish.	the in vivo and in vitro toxicity of bacterial cells and their extracellular products (ecps) from 16 strains of photobacterium damselae subsp. damselae isolated from 7 epizootic outbreaks were evaluated. on the basis of their 50% lethal dose (ld50) values (about 1 x 10(50 cfu), these strains may be considered as moderately virulent. however, their ecps were strongly lethal for redbanded seabream pagrus auriga causing fish death within 2 h post-inoculation (protein concentration ranged between 2. ...	2010	21166312
[characteristics of the response of natural and recombinant luminescent microorganisms in the presence of fe2+ ions].	it was found that divalent iron ions have alternative effects on the bioluminescence of the natural marine microorganism photobacterium phosphoreum and the recombinant escherichia coli strain with a cloned lux operon of p. leiognathi. in the presence of 0.25-5.0 mm feso4, the bioluminescence intensity of the former and the latter increased and decreased, respectively. to establish the causes of these differences, we studied the characteristics of the fatty acid composition of the compared microo ...	2010	20198914
[growth and bioluminescence of luminous bacteria under the action of aflatoxin b1 before and after its treatment with nanodiamonds].	the effect of aflatoxin b1 on growth and luminescence of marine luminous bacteria p. phosphoreum and recombinant e. coli z905 cells was investigated. the bidirectional effect of aflatoxin b1 on the studied bacterial species was detected--an inhibition of luminescence in p. phosphoreum and its stimulation in e. coli. it was shown that aflatoxin b1 influences the cell luminescence in the freshly grown cultures and bacteria restored after lyophilization. it was detected that the effect of aflatoxin ...	2010	20198915
occurrence and role of lactic acid bacteria in seafood products.	lactic acid bacteria (lab) in fish flesh has long been disregarded because the high post-mortem ph, the low percentage of sugars, the high content of low molecular weight nitrogenous molecules and the low temperature of temperate waters favor the rapid growth of ph-sensitive psychrotolerant marine gram-negative bacteria like pseudomonas, shewanella and photobacterium. in seafood packed in both vacuum (vp) and modified atmosphere (map) packaging commonly co(2) enriched, the growth of the gram-neg ...	2010	20630312
in situ aquaculture methods for dysidea avara (demospongiae, porifera) in the northwestern mediterranean.	marine sponges produce secondary metabolites that can be used as a natural source for the design of new drugs and cosmetics. there is, however, a supply problem with these natural substances for research and eventual commercialisation of the products. in situ sponge aquaculture is nowadays one of the most reliable methods to supply pharmaceutical companies with sufficient quantities of the target compound. in this study, we focus on the aquaculture of the sponge dysidea avara (schmidt, 1862), wh ...	2010	20631865
evaluation and structure-activity relationship study of acute toxicity of naphthoquinones to photobacterium phosphoreum, photobacterium t3b.	the acute toxicities of five naphthoquinone compounds to photobacterium phosphoreum were determined. we evaluated the mechanism of toxicity using the structure-activity relationship technique. the results showed that some factors, including the species of substituents, shape/size of molecule and oil-water partition coefficient (log p) played the important roles in the interaction between the naphthoquinones and the target. among of these, the toxicities of atovaquone and buparvaquone were lower ...	2010	20640400
isolation of photobacterium sp. lub-1 and its application in rapid assays for chemical toxicants in water.	to isolate marine bacteria with strong bioluminescence in a wide range of nacl concentration, especially at low salt conditions.	2010	20666988
cloning and sequencing of alginate lyase genes from deep-sea strains of vibrio and agarivorans and characterization of a new vibrio enzyme.	four alginate lyase genes were cloned and sequenced from the genomic dnas of deep-sea bacteria, namely members of vibrio and agarivorans. three of them were from vibrio sp. jam-a9m, which encoded alginate lyases, a9mt, a9mc, and a9ml. a9mt was composed of 286 amino acids and 57% homologous to alxm of photobacterium sp. a9mc (221 amino acids) and a9ml (522 amino acids) had the highest degree of similarity to two individual alginate lyases of vibrio splendidus with 74% and 84% identity, respective ...	2010	19941025
temporal shifts of the norway lobster (nephrops norvegicus) gut bacterial communities.	the aim of this study was to investigate the gut bacterial communities of nephrops norvegicus individuals, using a suite of molecular tools consisting of automated ribosomal intergenic spacer analysis, 16s rrna gene-internal transcribed spacer clone libraries and fish. the animals were collected from pagasitikos gulf, greece, during different months of the year. the diversity of the gut bacterial communities was found to mostly vary with sampling time, which could be related to temporal variatio ...	2010	20831590
evaluation of combined toxicity of phenols and lead to photobacterium phosphoreum and quantitative structure-activity relationships.	the combined toxicity of lead (pb) and nine phenols were measured. the result indicated that the combined toxicity is not only dependent on the pb concentrations but also on the positions of substituted groups of phenols. quantitative structure-activity relationship equations were built from the combined toxicity and physico-chemical descriptors of phenols in the different pb concentrations. the combined toxicity was related to water solubility and the third order molecular connectivity index (( ...	2010	20043147
a new effect residual ratio (err) method for the validation of the concentration addition and independent action models.	glutaraldehyde (ga) often acts as an effective sterilant, disinfectant, and preservative in chemical products. it was found that ga had clearly acute toxicity to aquatic organisms. furthermore, ga in natural environment could not exist as single species but as complex mixtures. to explore the toxicity interaction between ga and the other environmental pollutant, it is necessary to determine the mixture toxicities of various binary mixtures including ga. two reference models, concentration additi ...	2010	19949878
reduction kinetics of a flavin oxidoreductase luxg from photobacterium leiognathi (th1): half-sites reactivity.	bacterial bioluminescence is a phenomenon resulting from the reaction of a two-component fmn-dependent aldehyde monooxygenase system, which comprises a bacterial luciferase and a flavin reductase. bacterial luciferase (luxab) is one of the most extensively investigated two-component monooxygenases, while its reductase partner, the flavin reductase (luxg) from the same operon, has only been recently expressed in a functional form. this work reports transient kinetics identification of intermediat ...	2010	20836540
replacement of dietary fish oil by vegetable oils affects humoral immunity and expression of pro-inflammatory cytokines genes in gilthead sea bream sparus aurata.	commercial gilthead sea bream feeds are highly energetic, fish oil traditionally being the main lipid source. but the decreased fish oil production together with the increased prices of this oil encourages its substitution by vegetable oils, imposing new nutritional habits to aquaculture species. partial replacement of fish oil by vegetable oils in diets for marine species allows good feed utilization and growth but may affect fish health, since imbalances in dietary fatty acids may alter fish i ...	2010	20817101
sialyltransferases of marine bacteria efficiently utilize glycosphingolipid substrates.	bacterial sialyltransferases (sts) from marine sources were characterized using glycosphingolipids (gsls). bacterial sts were found to be beta-galacotoside sts. there were two types of sts: (1) st obtained from strains such as ishi-224, 05jtc1 (#1), ishi-467, 05jtd2 (#2), and faj-16, 05jte1 (#3), which form alpha2-3 sialic acid (sia) linkages, named alpha2-3st, (2) st obtained from strains such as ish-224, n1c0 (#4), pda-rec, 05jtb2 (#5), and pda-0160, 05jta2 (#6), which form alpha2-6 sia linkag ...	2010	19837852
an alpha2,6-sialyltransferase cloned from photobacterium leiognathi strain jt-shiz-119 shows both sialyltransferase and neuraminidase activity.	we cloned, expressed, and characterized a novel beta-galactoside alpha2,6-sialyltransferase from photobacterium leiognathi strain jt-shiz-119. the protein showed 56-96% identity to the marine bacterial alpha2,6-sialyltransferases classified into glycosyltransferase family 80. the sialyltransferase activity of the n-terminal truncated form of the recombinant enzyme was 1477 u/l of escherichia coli culture. the truncated recombinant enzyme was purified as a single band by sodium dodecyl sulfate po ...	2010	19797322
trans-sialidase activity of photobacterium damsela alpha2,6-sialyltransferase and its application in the synthesis of sialosides.	trans-sialidases catalyze the transfer of a sialic acid from one sialoside to an acceptor to form a new sialoside. alpha2,3-trans-sialidase activity was initially discovered in the parasitic protozoan trypanosoma cruzi, and more recently was found in a multifunctional pasteurella multocida sialyltransferase pmst1. alpha2,8-trans-sialidase activity was also described for a multifunctional campylobacter jejuni sialyltransferase cstii. we report here the discovery of the alpha2,6-trans-sialidase ac ...	2010	19880425
crystal structures of the lumazine protein from photobacterium kishitanii in complexes with the authentic chromophore, 6,7-dimethyl- 8-(1'-d-ribityl) lumazine, and its analogues, riboflavin and flavin mononucleotide, at high resolution.	lumazine protein (lump) is a fluorescent accessory protein having 6,7-dimethyl-8-(1'-d-ribityl) lumazine (dmrl) as its authentic chromophore. it modulates the emission of bacterial luciferase to shorter wavelengths with increasing luminous strength. to obtain structural information on the native structure as well as the interaction with bacterial luciferase, we have determined the crystal structures of lump from photobacterium kishitanii in complexes with dmrl and its analogues, riboflavin (rbf) ...	2010	19854891
molecular intraspecific characterization of photobacterium damselae ssp. damselae strains affecting cultured marine fish.	the aim of this study was to analyse the intraspecific variability of photobacterium damselae ssp. damselae strains isolated from different cultured marine fish species using molecular typing methods.	2010	19922594
development of a haemolysin gene-based multiplex pcr for simultaneous detection of vibrio campbellii, vibrio harveyi and vibrio parahaemolyticus.	to develop a haemolysin (hly) gene-based species-specific multiplex pcr for simple and rapid detection of vibrio campbellii, v. harveyi and v. parahaemolyticus.	2010	19943887
blacmy-2-positive inca/c plasmids from escherichia coli and salmonella enterica are a distinct component of a larger lineage of plasmids.	large multidrug resistance plasmids of the a/c incompatibility complex (inca/c) have been found in a diverse group of gram-negative commensal and pathogenic bacteria. we present three completed sequences from inca/c plasmids that originated from escherichia coli (cattle) and salmonella enterica serovar newport (human) and that carry the cephamycinase gene blacmy-2. these large plasmids (148 to 166 kbp) share extensive sequence identity and synteny. the most divergent plasmid, peh4h, has lost sev ...	2010	19949054
degradation of diazinon in apple juice by ultrasonic treatment.	the degradation of diazinon spiked in apple juice treated by ultrasonic treatment was investigated in this paper. results showed that the ultrasonic power and initial concentration of diazinon significantly influenced the degradation percentage of diazinon (p<0.05) and the degradation of diazinon followed the first-order kinetics model well. based on the gas chromatography-mass spectrometry (gc-ms) analysis, seven degradation products of diazinon have been identified. a degradation pathway invol ...	2010	20061173
photobacterium jeanii sp. nov., isolated from corals and zoanthids.	four novel isolates (r-40508(t), r-40507, r-40903 and r-21419) were obtained from different cnidarian species (phyllogorgia dilatata, merulina ampliata and palythoa caribaeorum) from different places in brazil and australia. the novel isolates formed a tight phylogenetic group based on 16s rrna, reca, topa, ftsz, mreb and rpoa gene sequences. their closest phylogenetic neighbours were the type strains of photobacterium leiognathi, p. rosenbergii and p. halotolerans, sharing 97.1-97.5 % 16s rrna ...	2010	20081016
establishment and characterization of a fibroblast cell line derived from the dorsal fin of red sea bream, pagrus major (temminck & schlegel).	the establishment and partial characterization of a continuous cell line from the dorsal fin of red sea bream, pagrus major, are described. the cell line, designated rsbf-2, has been subcultured for more than 100 passages since its initiation in november 2000. it was optimally maintained at 28 degrees c in leibovitz l-15 medium with 10% foetal bovine serum. propagation of rsbf-2 cells was serum dependent and exhibited low plating efficiency (<1.7%). aside from long-term cryopreservation, the cel ...	2010	20102463
differential characterization of biogenic amine-producing bacteria involved in food poisoning using maldi-tof mass fingerprinting.	histamine poisoning is caused by the consumption of fish and other foods that harbor bacteria possessing histidine decarboxylase activity. with the aim of preventing histamine formation, highly specific mass spectral fingerprints were obtained from the 16 major biogenic amine-producing enteric and marine bacteria by means of maldi-tof ms analysis. all bacterial strains analyzed exhibited specific spectral fingerprints that enabled its unambiguous differentiation. this technique also identified p ...	2010	20151397
insights into piezophily from genetic studies on the deep-sea bacterium, photobacterium profundum ss9.	the deep-sea bacterium, photobacterium profundum ss9, has been adopted as a model organism to understand the molecular basis of cold-adapted high-pressure-loving (piezophilic) growth. despite growing optimally at 28 mpa (15 degrees c), p. profundum ss9 can grow over a wide range of pressures and temperatures. the ability to grow at atmospheric pressure has enabled a limited set of genetic tools to be developed, which has provided genetic insights into the mechanism of piezophilic growth in p. pr ...	2010	20233381
comparisons of vibrio fischeri, photobacterium phosphoreum, and recombinant luminescent using escherichia coli as bod measurement.	to shorten the time needed to measure biochemical oxygen demand (bod) in water samples and to provide a rapid feedback of the real condition of water quality, we tested and evaluated the validity and reliability of luminescent bacteria vibrio fischeri, photobacterium phosphoreum, and recombinant escherichia coli as potential indicators of bod in the domestic wastewaters. the results indicate that the luminescence intensities of these strains are dependent on temperature, ph, and bod concentratio ...	2010	20390863
[evaluation of the phagocytic activity of human peripheral blood neutrophils, by employing recombinant luminescent bacteria].	the authors show it possible to evaluate the phagocytic activity of human peripheral blood neutrophils from their impact on the fluorescence intensity of recombinant luminescent bacteria. they have established the preferred use of recombinant luminescent escherichia coli bacteria with cloned luxcdabe genes of photobacterium leiognathii as phagocyted objects and substantiated the optimal method of their opsonization by normal human immunoglobulin. the variants of separate consideration of the lum ...	2010	20401994
antibacterial compounds from marine vibrionaceae isolated on a global expedition.	on a global research expedition, over 500 bacterial strains inhibitory towards pathogenic bacteria were isolated. three hundred of the antibacterial strains were assigned to the vibrionaceae family. the purpose of the present study was to investigate the phylogeny and bioactivity of five vibrionaceae strains with pronounced antibacterial activity. these were identified as vibrio coralliilyticus (two strains), v. neptunius (two strains), and photobacterium halotolerans (one strain) on the basis o ...	2010	21339958