Publications

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identification of new bacteria harboring qnrs and aac(6')-ib/cr and mutations possibly involved in fluoroquinolone resistance in raw sewage and activated sludge samples from a full-scale wwtp.wastewater treatment plants (wwtps) harbor bacteria and antimicrobial resistance genes, favoring gene exchange events and resistance dissemination. here, a culture-based and metagenomic survey of qnra, qnrb, qnrs, and aac(6')-ib genes from raw sewage (rs) and activated sludge (as) of a full-scale municipal wwtp was performed. a total of 96 bacterial isolates were recovered from nalidixic acid-enrichment cultures. bacteria harboring the aac(6')-ib gene predominated in rs, whereas qnrs-positive is ...201727984803
clonal transmission of gram-negative bacteria with carbapenemases ndm-1, vim-1, and oxa-23/72 in a bulgarian hospital.we characterized 72 isolates with reduced susceptibility to carbapenems (50 acinetobacter spp., 13 proteus mirabilis, five escherichia coli, one morganella morganii, one enterobacter cloacae, one providencia rettgeri, and one pseudomonas aeruginosa) from a hospital in sofia, bulgaria. different β-lactamase genes were identified by polymerase chain reaction and sequencing. bacterial strain typing was performed by enzymatic macrorestriction and pulsed-field gel electrophoresis (pfge) typing as wel ...201727459019
detection of sgi1/pgi1 elements and resistance to extended-spectrum cephalosporins in proteae of animal origin in france.proteae, and especially proteus mirabilis, are often the cause of urinary tract infections (utis) in humans. they were reported as carriers of extended-spectrum β-lactamase (esbl) genes, and recently of carbapenemases, mostly carried by the salmonella genomic island 1 (sgi1) and proteus genomic island 1 (pgi1). proteae have also lately become an increasing cause of utis in companion animals, but antimicrobial susceptibility data in animals are still scarce. here, we report the characterization o ...201728154560
first complete providencia rettgeri genome sequence, the ndm-1-producing clinical strain rb151.providencia rettgeri is an opportunistic bacterial pathogen of clinical significance due to its association with urinary tract infections and multidrug resistance. here, we report the first complete genome sequence of p. rettgeri the genome of strain rb151 consists of a 4.8-mbp chromosome and a 108-kbp blandm-1-positive plasmid.201728104655
the genetic architecture of defence as resistance to and tolerance of bacterial infection in drosophila melanogaster.defence against pathogenic infection can take two forms: resistance and tolerance. resistance is the ability of the host to limit a pathogen burden, whereas tolerance is the ability to limit the negative consequences of infection at a given level of infection intensity. evolutionarily, a tolerance strategy that is independent of resistance could allow the host to avoid mounting a costly immune response and, theoretically, to avoid a co-evolutionary arms race between pathogen virulence and host r ...201728099780
expression of an acid urease with urethanase activity in e. coli and analysis of urease gene.urea in alcoholic beverage is a precursor of ethyl carbamate (ec), which is carcinogenic. enzymatic elimination of urea has attracted much research interest. acid urease with good tolerance toward ethanol and acid is ideal enzyme for such applications. in the present work, the structural genes of urease from providencia rettgeri jn-b815, ureabc were efficiently expressed in e. coli bl21(de3) in an active form (apourease) exhibiting both urease and urethanase (hydrolyze ec) activities. the specif ...201728197768
rna interference directed against the transglutaminase gene triggers dysbiosis of gut microbiota in drosophila.we recently reported that transglutaminase (tg) suppresses immune deficiency pathway-controlled antimicrobial peptides (imd-amps), thereby conferring immune tolerance to gut microbes, and that rnai of the tg gene in flies decreases the lifespan compared with non-tg-rnai flies. here, analysis of the bacterial composition of the drosophila gut by next-generation sequencing revealed that gut microbiota comprising one dominant genus of acetobacter in non-tg-rnai flies was shifted to that comprising ...201627760824
outbreak of carbapenem-resistant providencia rettgeri in a tertiary hospital.the emergence of resistance to multiple antimicrobial agents in pathogenic bacteria is a significant public health threat, as there are limited effective antimicrobial agents for infections caused by multidrug-resistant (mdr) bacteria. several mdr bacteria are now frequently detected. carbapenem resistance in enterobacteriaceae is often plasmid mediated, necessitating stringent infection control practices. wedescribe an outbreak of carbapenem-resistant providencia rettgeri involving 4 patients a ...201628112086
nitrogen removal by providencia rettgeri strain yl with heterotrophic nitrification and aerobic denitrification.providencia rettgeri strain yl shows the capability of nitrogen removal under sole aerobic conditions. by using isotope ratio mass spectrometry, (15)n-labelled n2o and n2 were detected in aerobic batch cultures containing [formula: see text], [formula: see text] or [formula: see text]. strain yl converted [formula: see text], [formula: see text] and [formula: see text] to produce more n2o than n2 in the presence of [formula: see text]. an (15)n isotope tracing experiment confirmed that the nitro ...201626824874
preparation of crosslinked enzyme aggregates (cleas) of acid urease with urethanase activity and their application.an acid urease from providencia rettgeri jn-b815 was purified via ultrasonication, ethanol precipitation, and deae ion-exchange column chromatography. it was found that the enzyme exhibits not only urease activity, but also urethanase activity, which made it possible to reduce ec already existed or would produce and its precursor urea at the same time. then, crosslinked enzyme aggregates of p. rettgeri urease (pru-cleas) were prepared using genipin as crosslinking agent. the purification process ...201626627914
ndm-producing enterobacteriaceae in a chinese hospital, 2014-2015: identification of ndm-producing citrobacterwerkmanii and acquisition of blandm-1-carrying plasmid in vivo in a clinical escherichia coli isolate.new delhi metallo-β-lactamase (ndm)-producing enterobacteriaceae (npe) shows prevalence in china. little is known about the mechanisms related to the spread of npe. recently, a total of 51 non-duplicated npe isolates were collected from a tertiary-care hospital in china and analysed for genetic relatedness by pfge, antimicrobial susceptibility by etest and sequence type by multilocus sequence typing. s1-pfge and southern blot analysis or pcr amplification were used for plasmid profiling. between ...201627667823
characterization of carbapenem-resistant gram-negative bacteria from tamil nadu.carbapenem resistance is disseminating worldwide among gram-negative bacteria. the aim of this study was to identify carbapenem-resistance level and to determine the mechanism of carbapenem resistance among clinical isolates from two centres in tamil nadu. in the present study, a total of 93 gram-negative isolates, which is found to be resistant to carbapenem by disk diffusion test in two centres, were included. all isolates are identified at species level by 16s rrna sequencing. minimal inhibit ...201626198414
plant growth promoting potential and phylogenetic characteristics of a lichenized nitrogen fixing bacterium, enterobacter cloacae.lichens are complex symbiotic association of mycobionts, photobionts, and bacteriobionts, including chemolithotropic bacteria. in the present study, 46 lichenized bacteria were isolated by conventional and enrichment culture methods on nitrogen-free bromothymol blue (nfb) medium. only 11 of the 46 isolates fixed nitrogen on nfb and had reduced acetylene. all these 11 isolates had also produced siderophore and 10 of them the iaa. further, ammonia production was recorded from nine of these nitroge ...201627273065
colistin-nonsusceptible pseudomonas aeruginosa sequence type 654 with blandm-1 arrives in north america.this study describes 3 different blandm-1 genetic platforms in 3 different species obtained from the same patient who was directly transferred to an institution in calgary, alberta, canada, following a prolonged hospital stay in india. the blandm-1 in the escherichia coli isolate was located on a 176-kb inca/c plasmid contained within an iscr1 region. the blandm-1 in the providencia rettgeri isolate was located on a 117-kb inct plasmid contained within tn3000, while the blandm-1 in the pseudomon ...201626824951
pyriformsinusosotomy. a useful technique for temporary or permanent tube feeding. 20164959520
bacterial volatiles attract gravid secondary screwworms (diptera: calliphoridae).bovine blood inoculated and incubated with bacteria was tested to determine if secondary screwworm, cochliomyia macellaria (f.), would be attracted to the incubated substrate for oviposition. five species of bacteria, klebsiella oxytoca (flugge), proteus mirabilis hauser, proteus vulgaris hauser, providencia rettgeri hadley, elkins and caldwell, and providencia stuartii ewing, previously isolated from animal wounds infested by primary screwworms, cochliomyia hominivorax (coquerel), were used. in ...201626748982
letter: long-term gold salt therapy. 20164832169
incidence of primary carcinoma of the ureter: a report of five cases. 20165005322
supplementary impressions for crowns and bridges. 20164912412
[autoimmune hepatitis]. 20164865241
spinal fluid cytomorphology. description of apparatus, technique, and findings. 20164862475
[bacterial endocarditis associated with active rheumatic cardiopathy. anatomo-clinical correlation of 30 cases]. 20164750703
a new look at respiratory illness in general practice. a reclassification of respiratory illness based on antibiotic prescribing. 20164785756
[dynamics of changes of gastric hydrochloric acid during the course of chronic obstructive lung disease]. 20164753752
a histologic reappraisal of the thymus in myasthenia gravis. a correlative study of thymic pathology and response to thymectomy. 20164922629
[diazocopy for duplication of radiographs (author's transl)]. 20164822248
group exposure (flooding) in vivo for agoraphobics. 20164852186
one successful staffing pattern for a methadone maintenance clinic. 20164808146
[problem of mixed gonorrheal-trichomonad urethritis in men]. 20164767956
mineralization of the bone in an insured population: correlation with reported fractures and other measures of osteoporosis. 20164776018
[breast augmentation prostheses. critical study of the results]. 20164775918
carcinoma of breast. influence of size of primary lesion and lymph node involvement based on selective biopsy. 20165045884
treatment of gastrointestinal cancer: current status and recent progress. 20164860338
serum enzyme levels during exercise in patients with coronary heart disease: effects of training. 20165075087
effects of propranolol and isosorbide dinitrate on exercise performance and adrenergic acitivity in patients with angina pectoris. 20164884575
drug induced retinopathy. 20164902422
cycloheximide induced amnesia and recovery as a function of training parameters. 20164857294
protein-alkaloid relationship in datura stramonium var. tatula. 20165050374
asthmatic hyperresponsiveness. 20164885824
prevalence and antimicrobial resistance pattern of bacterial strains isolated from patients with urinary tract infection in messalata central hospital, libya.to investigate the prevalence of urinary tract infection among patients at messalata central hospital, libya, to identify the causative bacteria, and to explore their resistance pattern to antimicrobials.201627569886
towards a physical understanding of physiological excitation as a cooperative specific adsorption phenomenon. 20164783696
benjamin rush, m. d. (1745-1813). 20164875618
[investigations on the central respiratory activity of canrenoate-k (author's transl)]. 20164749520
[experience with ultraviolet irradiation of the blood in chronic diseases]. 20164837149
[detection of enzymatic changes in biopsy samples from patients with cortisone myopathies (phophorylase activity)]. 20154432040
myxoedema. 20154554481
[urologic diseases in the newborn]. 20154642498
psychiatric considerations in the sexual reassignment of non-intersex individuals. 20154426161
lipid disorders in diabetes mellitus. 20154626314
sodium cyanate: chemical properties relevant to sickle cell disease therapy. 20154752126
sodium cyanate: chemical properties relevant to sickle cell disease therapy. 20154752126
apathetic hyperthyroidism presenting as tricuspid regurgitation. 20154703632
apathetic hyperthyroidism presenting as tricuspid regurgitation. 20154703632
genomic plasticity of multidrug-resistant ndm-1 positive clinical isolate of providencia rettgeri.we performed a detailed whole-genome sequence analysis of providencia rettgeri h1736, a multidrug-resistant clinical pathogen isolated in israel in 2011. the objective was to describe the genomic flexibility of this bacterium that has greatly contributed to its pathogenicity. the genome has a chromosome size of 4,609,352 bp with 40.22% gc content. five plasmids were predicted, as well as other mobile genetic elements (mges) including phages, genomic islands, and integrative and conjugative eleme ...201527386606
[adamantiades--beh cet syndrome (report of 4 cases)]. 20154510738
[radiomorphology of renal tuberculosis with arguments for a simplified stage classification with reference to therapeutic viewpoints]. 20154344509
optical scatter patterns facilitate rapid differentiation of enterobacteriaceae on chromagar™ orientation medium.enterobacteriaceae family comprised pathogens and commensals and has a significant impact on food safety and public health. enterobacteriaceae is often enumerated and presumptively identified on chromogenic media, such as chromagar(tm) orientation medium based on colony profile; however, classification is highly arbitrary, and some could not be differentiated due to similar chromogen production. here, we investigated the ability of the laser optical sensor, bardot (bacterial rapid detection usin ...201526503189
biodegradation and detoxification of textile azo dyes by bacterial consortium under sequential microaerophilic/aerobic processes.release of textile azo dyes to the environment is an issue of health concern while the use of microorganisms has proved to be the best option for remediation. thus, in the present study, a bacterial consortium consisting of providencia rettgeri strain hsl1 and pseudomonas sp. suk1 has been investigated for degradation and detoxification of structurally different azo dyes. the consortium showed 98-99 % decolorization of all the selected azo dyes viz. reactive black 5 (rb 5), reactive orange 16 (r ...201526417357
newer diagnostic methods. 20154453578
implantation of permanent pacemaker in the first month of life for congenital complete heart block. 20154704237
latent carcinoma of the prostate--why the controversy? 20154823973
[individuation and communication in various neurosis structures]. 20154692550
latent carcinoma of the prostate--why the controversy? 20154823973
[the analysis of systolic intervals in the diagnosis of ischemic heart disease (author's transl)]. 20154451917
significance of eosinophilia during gold therapy. 20154433354
[results of the surgical treatment of cancers of the transverse colon. apropos of 43 cases]. 20154652093
acedapsone in leprosy treatment: trial in 68 active cases in micronesia. 20154672994
[hybernoma as a cause of cervicocranial syndrome]. 20154755241
the population-genetic aspect of forest typology. 20154432134
mental retardation associated with an unusual amino acid excretion pattern. 20154426131
[clinical aspects and pathogenesis of sudden deafness]. 20154463611
[existence of a nucleotide pyrophosphatase in germinated fenugreek seeds]. 20154350566
[organization of dental care to the urban population based on the principle of division by districts]. 20154501426
[old age and society]. 20154451919
nad-dependent alpha-glycerophosphate dehydrogenase in the housefly, musca domestica. 20154335910
a pleomorphic adenoma (so-called mixed tumor) in the wall of a dentigerous cyst. 20154351329
histochemical observations on the degenerating eggs and interstitial gland cells of the ovary of branchiostoma. 20154649174
[manual and automatic microdetermination of p-aminohippuric acid in the plasma and urine]. 20154671790
effect of exercise upon plasma cortisol and airway obstruction in asthmatic children. 20154720409
effect of exercise upon plasma cortisol and airway obstruction in asthmatic children. 20154720409
[surgical treatment of primary bronchopulmonary cancers]. 20154652111
coagulation problems. 20154576124
[electronic reading machines and orientation aids for the blind (author's transl)]. 20154609722
[results of controlled ophthalmologic studies during hormonal contraception using ovosiston]. 20155064445
international council of nurses voices nurses' support on the limitation of smoking. 20154489591
ethambutol in the re-treatment of multi-resistant advanced pulmonary tuberculosis (salvage cases). 20154422083
the paraproteinemias. ii. 20154380090
growth hormone levels in slipped capital femoral epiphysis. 20154652052
[nurse's awareness in the nurse-patient interaction--experience in the psychiatric ward]. 20154483738
reference levels for corticosterone and immune function in farmed saltwater crocodiles (crocodylus porosus) hatchlings using current code of practice guidelines.to determine reference levels for on-farm stressors on immune responsiveness and growth rate, 253 hatchling crocodiles from 11 known breeding pairs were repeatedly measured and blood sampled during their first year. plasma corticosterone (cort) was used to quantify baseline stress levels in captive animals and were found to be lower (mean 1.83±se 0.16 ng/ml) than previously reported in saltwater crocodile hatchlings. two tests of immune function were also conducted. innate constitutive immunity ...201525644211
sexual maturation in juvenile hypothyroidism. 20154357385
genomic characteristics of ndm-producing enterobacteriaceae isolates in australia and their blandm genetic contexts.blandm has been reported in different enterobacteriaceae species and on numerous plasmid replicon types (inc). plasmid replicon typing, in combination with genomic characteristics of the bacterial host (e.g., sequence typing), is used to infer the spread of antimicrobial resistance determinants between genetically unrelated bacterial hosts. the genetic context of blandm is heterogeneous. in this study, we genomically characterized 12 ndm-producing enterobacteriaceae isolated in australia between ...201526482302
draft genome sequences of three ndm-1-producing enterobacteriaceae species isolated from brazil.the emergence of multidrug-resistant enterobacteriaceae strains producing carbapenemases, such as ndm-1, has become a major public health issue due to a high dissemination capacity and limited treatment options. here we describe the draft genome of three ndm-1-producing isolates: providencia rettgeri (ccbh11880), enterobacter hormaechei subsp. oharae (ccbh10892) and klebsiella pneumoniae (ccbh13327), isolated in brazil. besides blandm-1, resistance genes to aminoglycosides [aada1, aada2, aac(6') ...201526061151
chemical composition and antibacterial activity of lavandula coronopifolia essential oil against antibiotic-resistant bacteria.the aim of this study was to analyse the composition of the essential oil (eo) of lavandula coronopifolia from morocco and to evaluate its in vitro antibacterial activity against antibiotic-resistant bacteria isolated from clinical infections. the antimicrobial activity was assessed by a broth micro-well dilution method using multiresistant clinical isolates of 11 pathogenic bacteria: klebsiella pneumoniae subsp. pneumoniae, klebsiella ornithinolytica, escherichia coli, enterobacter cloacae, ent ...201525174508
a case of wound infection with providencia rettgeri and coincident gout in a patient from guam.providencia rettgeri (p. rettgeri) is a ubiquitous organism that is infrequently associated with human disease. here we report the isolation of this organism from a polymicrobial wound infection resulting from ruptured tophi on a 54-year-old male patient from guam. we describe the identification and confirmation of this organism, and propose metabolic synergy as a possible mechanism of pathogenesis. to our knowledge, this is the first published report of a wound infection colonized by p. rettger ...201526568901
first report of a ndm-producing providencia rettgeri strain in the state of são paulo. 201526432948
[emergence of providencia rettgeri ndm-1 in two departments of colombia, 2012-2013].in colombia, between 2012 and 2013, 19 isolates with ndm were identified, of which 14 corresponded to providencia rettgeri.201526130312
first case of ndm-1-producing providencia rettgeri in ecuador. 201527842879
nosocomial dissemination of plasmids carrying blatem-24, bladha-1, aac(6')-ib-cr, and qnra6 in providencia spp. strains isolated from a tunisian hospital.the aim of this study is to report the emergence of inca/c conjugative plasmids harboring blatem-24, bladha-1, qnra6, and aac(6')-ib-cr genes among providencia spp. isolates recovered in 2008 in tunisia. the double-disk synergy test confirmed the phenotype extended-spectrum β-lactamase (esbl) in 2 providencia stuartii and 5 providencia rettgeri. these esbls were coresistant to cefoxitin, chloramphenicol, ofloxacin, and sulfonamides but remained susceptible to imipenem. three β-lactamases tem-2, ...201525315769
replication and active partition of integrative and conjugative elements (ices) of the sxt/r391 family: the line between ices and conjugative plasmids is getting thinner.integrative and conjugative elements (ices) of the sxt/r391 family disseminate multidrug resistance among pathogenic gammaproteobacteria such as vibrio cholerae. sxt/r391 ices are mobile genetic elements that reside in the chromosome of their host and eventually self-transfer to other bacteria by conjugation. conjugative transfer of sxt/r391 ices involves a transient extrachromosomal circular plasmid-like form that is thought to be the substrate for single-stranded dna translocation to the recip ...201526061412
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