Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
characterisation of manganese peroxidase and laccase producing bacteria capable for degradation of sucrose glutamic acid-maillard reaction products at different nutritional and environmental conditions.maillard reactions products (mrps) are a major colorant of distillery effluent. it is major source of environmental pollution due to its complex structure and recalcitrant nature. this study has revealed that sucrose glutamic acid-maillard reaction products (sga-mrps) showed many absorption peaks between 200 and 450 nm. the absorption maximum peak was noted at 250 nm in spectrophotometric detection. this indicated the formation of variable molecular weight maillard products during the sga-mrps f ...201829396692
identification of eayjjpb encoding a dicarboxylate transporter important for succinate production under aerobic and anaerobic conditions in enterobacter aerogenes.enterobacter aerogenes, a gram-negative, rod-shaped bacterium, is an effective producer of succinate from glucose via the reductive tricarboxylic acid cycle under anaerobic conditions. however, to date, succinate-exporter genes have not been identified in e. aerogenes, although succinate exporters have a large impact on fermentative succinate production. recently, we genetically identified yjjp and yjjb, as genes encoding a succinate transporter in escherichia coli. evaluation of the yjjpb homol ...201829395959
black zira essential oil: chemical compositions and antimicrobial activity against the growth of some pathogenic strain causing infection.the aim of this study was to perform chemical compositions and phytochemical analysis of black zira essential oil and other goal of this research was to investigate the antimicrobial effects of black zira essential oil against enterobacter aerogenes, pseudomonas aeruginosa, escherichia coli, shigella flexneri, staphylococcus epidermidis, streptococcus pyogenes and candida albicans. black zira essential oil was extracted by hydrodistillation method using clevenger apparatus. black zira essential ...201829360566
preparation and evaluation of antibacterial potential of pithecellobium dulce root extract against gram positive and gram negative bacteria.in the present study hexane, benzene, ethyl acetate and ethanol extracts of pithecellobium dulce root were prepared using soxhlet extractor. the extracts were evaluated for antibacterial activity against one gram positive (staphylococcus aureus) and three gram negative (acetobacter aceti, acetobacter aceti, klebsiella pneumoniae) strains. disc diffusion method revealed promising antibacterial activity of the extracts prepared in polar solvents (ethyl acetate and ethanol) compared to non-polar so ...201829331370
synthesis, anti-microbial activity, cytotoxicity of some novel substituted (5-(3-(1h-benzo[d]imidazol-2-yl)-4-hydroxybenzyl)benzofuran-2-yl)(phenyl)methanone analogs.there is a dire need for the discovery and development of new antimicrobial agents after several experiments for a better resistance of microorganisms towards antimicrobial agents become a serious health problem for a few years in the past. as benzimidazole possess various types of biological activities, it has been synthesized, in the present study, a new series of (5-(3-(1h-benzo[d]imidazol-2-yl)-4-hydroxybenzyl)benzofuran-2-yl)(phenyl)methanone analogs by using the condensation and screened f ...201829318401
impact of heavy metals on hydrogen production from organic fraction of municipal solid waste using co-culture of enterobacter aerogenes and e. coli.in the present study, the effect of heavy metals (lead, mercury, copper, and chromium) on the hydrogen production from the organic fraction of municipal solid waste (ofmsw) was investigated using co-culture of facultative anaerobes enterobacter aerogenes and e. coli. heavy metals were applied at concentration range of 0.5, 1, 2, 5, 10, 20, 50 and 100 mg/l. the results revealed that lead, mercury, and chromium negatively affected hydrogen production for the range of concentrations applied. applic ...201829426722
antibacterial, and antioxidant potentials of non-cytotoxic extract of trichoderma atroviride.trichoderma species are a rich source of metabolites, but less known for biomedical potential. this work deals with antibacterial and antioxidant potentials of intracellular non-cytotoxic metabolites, extracted from trichoderma atroviride (knup001). a total of 53 fractions was collected by column chromatography and tested for cytotoxicity by mtt assay. only one fraction (f41) was found to be non-toxic to vero cells with 95.4 ± 0.61% of survival. the f41 was then subjected to chemical analysis, a ...201829307755
ram locus is a key regulator to trigger multidrug resistance in enterobacter aerogenes.several genetic regulators belonging to arac family are involved in the emergence of mdr isolates of e. aerogenes due to alterations in membrane permeability. compared with the genetic regulator mar, rama may be more relevant towards the emergence of antibiotic resistance.201829297851
antibacterial activities, phytochemical analysis and chemical composition makhlaseh extracts against the growth of some pathogenic strain causing poisoning and infection.the aim of this study was to investigate the antibacterial activities and phytochemical analysis of extracts against the growth of some pathogenic strain causing poisoning and infection (staphylococcus aureus, streptococcus pyogenes, staphylococcus epidermidis, enterobacter aerogenes, escherichia coli and shigella flexneri). makhlaseh components were identified via gas chromatography/mass spectrometry (gc/ms). total phenolic content (tpc), alkaloids, tannins and saponins were determined. antioxi ...201829203365
risk factors and microbiology of meningitis and/or bacteremia after transsphenoidal surgery for pituitary adenoma.to investigate the incidence, bacteriologic features, and risk factors of posttranssphenoidal surgery (post-tss) meningitis and/or bacteremia.201829191537
isolation and characterization of antibiotic producing bacterial strains from red soil of himalayan region of pakistan.the emergence of multi drug resistant microbial pathogens has become a global health challenge and set a dire requirement of searching new effective antimicrobials. soil is an ultimate reservoir of biologically active micro flora, which harbors trillions of microbial strains producing compounds of commercial interest. hence aim of the present study was an attempt to isolate and identify the antibiotic producing microbial strains from the red soil of himalayan an unexplored region of pakistan. in ...201729188775
infections caused by antimicrobial drug-resistant saprophytic gram-negative bacteria in the environment.drug-resistance genes found in human bacterial pathogens are increasingly recognized in saprophytic gram-negative bacteria (gnb) from environmental sources. the clinical implication of such environmental gnbs is unknown.201729164118
mixed bacterial culture mediated direct conversion of bio-glycerol to diols.direct and economic transformation of biodiesel derived crude glycerol is gaining more significance. during screening of bacterial cultures klebsiella pneumoniae and enterobacter aerogenes were able to convert crude bio-glycerol to 2,3-butanediol (2,3-bdo) and 1,3-propanediol (1,3-pdo), as major compounds, ethanol and acetoin as minor compounds, with a conversion of 69% and 79% respectively. process optimization could achieve maximum conversion at ph 7.0, 37 °c, 30-40 g/l glycerol and 1.5 g of i ...201729156369
in vitro effects of inulin and soya bean oligosaccharide on skatole production and the intestinal microbiota in broilers.the experiment was conducted to investigate the in vitro effects of inulin and soya bean oligosaccharide (sbo) on the metabolism of l-tryptophan (l-try) to skatole production, and the intestinal microbiota in broilers. treatments were as follows: caecal microbiota control (cc), cc + inulin, cc + sbo, rectal microbiota control (rc), rc + inulin and rc + sbo. microbial suspensions were anaerobically incubated at 38°c for 24 hr. the results showed that concentrations of skatole and acetic acid were ...201729105163
impairment of nadh dehydrogenase and regulation of anaerobic metabolism by the small rna ryhb and nade for improved biohydrogen production inenterobacter aerogenes.enterobacter aerogenesis a facultative anaerobe and is one of the most widely studied bacterial strains because of its ability to use a variety of substrates, to produce hydrogen at a high rate, and its high growth rate during dark fermentation. however, the rate of hydrogen production has not been optimized. in this present study, three strategies to improve hydrogen production ine. aerogenes, namely the disruption ofnuocde, overexpression of the small rna ryhb and of nade to regulate global an ...201729093752
emergence of colistin resistance in enterobacter aerogenes from croatia.a colistin-resistant enterobacter aerogenes [study code 12264] was isolated from the tracheal aspirate of a 71-year-old male patient in the general hospital [gh] in pula, croatia. the patient was previously treated in university hospital centre in rijeka with colistin in order to eradicate acinetobacter baumannii isolate, susceptible only to colistin and tigecycline. genes encoding esbls [blatem, blashv, blactx-m, blaper-1] were screened by pcr. the strain was shown to possess blactx-m-15and bla ...201729063811
infection rate in adult patients with open fractures treated at the emergency hospital and at the ulbra university hospital in canoas, rio grande do sul, brazil.to identify the infection rate in adult patients with open fractures treated at two tertiary hospitals in the city of canoas, rio grande do sul, brazil.201729062818
[results of a multicenter study investigating plasmid mediated colistin resistance genes (mcr-1 and mcr-2) in clinical enterobacteriaceae ısolates from turkey].colistin is a polymyxin antibiotic which is considered as one of the last line agents against infections due to multidrug resistant or carbapenem resistant gram-negative pathogens. colistin resistance is associated with chromosomal alterations which can usually cause mutations in genes coding specific two component regulator systems. the first plasmid-mediated colistin resistance gene, mcr-1 was described in escherichia coli and klebsiella pneumoniae isolates in december 2015 and followed by ano ...201728929967
biological and physicochemical properties of biosurfactants produced by lactobacillus jensenii p6aand lactobacillus gasseri p65.lactobacillus species produce biosurfactants that can contribute to the bacteria's ability to prevent microbial infections associated with urogenital and gastrointestinal tracts and the skin. here, we described the biological and physicochemical properties of biosurfactants produced by lactobacillus jensenii p6aand lactobacillus gasseri p65.201728927409
screening, nutritional optimization and purification for phytase produced by enterobacter aerogenes and its role in enhancement of hydrocarbons degradation and biofilm inhibition.in this study, a novel isolate of enterobacter aerogenes isolated from contaminated soils with hydrocarbons had extracellular phytate-degrading activity. enterobacter aerogenes isolates were identified by biochemical tests and confirmed by16s rrna gene products (amplified size 211bp) for genotypic detection. the phytase activity was reached to maximum activity when this isolate was cultivated under the optimal conditions which consisted of using minimal salt medium containing 1%(w/v) rice bran a ...201729269246
prevalence of gram-negative bacilli isolated from the equipment and surfaces in hospital wards of golestan province, north of iran.nosocomial infections are one of the most common causes of death in hospitals. this study aimed to determine the prevalence of gram-negative bacilli isolated from the equipment in hospital wards of the golestan province, in the year 2015. in this cross-sectional study in 2015, 1980 samples from medical and nonmedical equipment and surfaces were collected from the wards of 13 teaching hospitals, in the golestan province. samples were inoculated into eosin methylene blue agar and blood agar cultur ...201729403653
antibacterial activity of 1-[(2,4-dichlorophenethyl)amino]-3-phenoxypropan-2-ol against antibiotic-resistant strains of diverse bacterial pathogens, biofilms and in pre-clinical infection models.we recently described the novel anti-persister compound 1-[(2,4-dichlorophenethyl)amino]-3-phenoxypropan-2-ol (spi009), capable of directly killing persister cells of the gram-negative pathogenpseudomonas aeruginosa. this compound also shows antibacterial effects against non-persister cells, suggesting that spi009 could be used as an adjuvant for antibacterial combination therapy. here, we demonstrate the broad-spectrum activity of spi009, combined with different classes of antibiotics, against ...201729312259
isolation and identification of nonoral pathogenic bacteria in the oral cavity of patients with removable dentures.dentures in the oral cavity may act as a reservoir of microorganisms, which may be related to systemic infections. the aim of this study was to investigate the nonoral pathogenic bacteria in the oral cavity of patients with removable dentures in shiraz, southern iran.201728852636
low prevalence of carbapenem-resistant enterobacteriaceae among wounded military personnel.multidrug-resistant organisms (mdros) are a global health problem that affect both civilian and military populations. among wounded warriors, mdros further complicate the care of trauma-related infections, resulting in extended duration of hospitalization, as well as increased morbidity and mortality. during the wars in iraq and afghanistan, extended spectrum β-lactamase-producing enterobacteriaceae were frequently isolated from wounded warriors. the potential emergence of difficult-to-treat car ...201728853114
trends in antimicrobial resistance against enterobacteriaceae strains isolated from blood: a 10-year epidemiological study in mainland china (2004-2014).antimicrobial resistance is a serious problem that compromises the empirical treatment of infections, resulting in a lack of effective antibiotics and high medical expenses. here, we aimed to monitor the trends in antimicrobial resistance among enterobacteriaceae isolated from blood samples in mainland china.201728836547
perturbation of formate pathway and nadh pathway acting on the biohydrogen production.the formate pathway and nadh pathway as two common hydrogen-producing metabolic pathways have been well characterized to understand and improve biohydrogen production. these two pathways have been thought to be separate and have been independently investigated. however, in this study, perturbation of genes (hyca, fdhf, fhla, ldha, nuob, hybo, fdh1, narp, and ppk) in enterobacter aerogenes related to the formate pathway or nadh pathway revealed that these two pathways affected each other. further ...201728852065
whole genome of klebsiella aerogenes px01 isolated from san jacinto river sediment west of baytown, texas reveals the presence of multiple antibiotic resistance determinants and mobile genetic elements.klebsiella aerogenes is a gram-negative bacterium of the family enterobacteriaceae which is widely distributed in water, air and soil. it also forms part of the normal microbiota found in human and animal gastrointestinal tracts. here we report the draft genome sequence (chromosome and 1 plasmid) of k. aerogenes strain px01 compiled at the scaffold level from 97 contigs totaling 5,262,952 bp. k. aerogenes px01 was isolated from sediment along the northern face of burnet bay west of baytown, texa ...201728794987
update of incidence and antimicrobial susceptibility trends of escherichia coli and klebsiella pneumoniae isolates from chinese intra-abdominal infection patients.to evaluate in vitro susceptibilities of aerobic and facultative gram-negative bacterial (gnb) isolates from intra-abdominal infections (iais) to 12 selected antimicrobials in chinese hospitals from 2012 to 2014.201729254478
incidence and consequences of near-drowning-related pneumonia-a descriptive series from martinique, french west indies.drowning represents one major cause of accidental death. near-drowning patients are exposed to aspiration that may result in pneumonia with life-threatening consequences. we designed this descriptive study to investigate the frequency, nature, and consequences of post-drowning pneumonia. one hundred and forty-four near-drowning patients (33 children and 111 adults) admitted during four years to the university hospital of martinique, french indies, were included. patients presented pre-hospital c ...201729149019
chemical composition, antioxidant, and antibacterial activity of essential oils from etlingera sayapensis a.d. poulsen & ibrahim.to report the chemical composition and bioactivity (including antioxidant and antimicrobial activity) of essential oils from the rhizomes, stems, and leaves of etlingera sayapensis (e. sayapensis) a.d. poulsen & ibrahim for the first time.201728942832
assessment of two carrier materials for phosphate solubilizing biofertilizers and their effect on growth of wheat (triticum aestivum l.).biofertilizers are usually carrier-based inoculants containing beneficial microorganisms. incorporation of microorganisms in carrier material enables easy-handling, long-term storage and high effectiveness of biofertilizers. objective of the present study was to assess enriched biogas sludge and soil as biofertilizer carriers on growth and yield of wheat. six phosphate solubilizing strains were used in this study. three phosphate solubilizing strains, 77-ns2 (bacillus endophyticus), 77-cs-s1 (ba ...201728942836
identification of ideal multi-targeting bioactive compounds against mur ligases of enterobacter aerogenes and its binding mechanism in comparison with chemical inhibitors.enterobacter aerogenes have been reported as important opportunistic and multi-resistant bacterial pathogens for humans during the last three decades in hospital wards. the emergence of drug-resistant e. aerogenes demands the need for developing new drugs. peptidoglycan is an important component of the cell wall of bacteria and the peptidoglycan biochemical pathway is considered as the best source of antibacterial targets. within this pathway, four mur ligases murc, murd, mure, and murf are resp ...201729086207
green synthesis of silver nanoparticles from grape and tomato juices and evaluation of biological activities.the biosynthesis of silver nanoparticles (agnps) is substantial for its application in lots of fields. tomato and grape fruit juices were used as a reducing and capping agents for the biosynthesis of agnps. ultraviolet spectroscopic analysis offered peaks in the range of 396‒420 nm that indicate the production of agnps. fourier transform infrared spectroscopy analysis revealed attachment of different functional groups with ag ion in both tomato and grape fruit extracts nps. the x‒ray diffraction ...201728477003
draft genome sequence of a multidrug-resistant kpc-2-producing enterobacter aerogenes isolated from a hospitalized patient, brazil.multidrug-resistant (mdr) enterobacter aerogenes strains are frequently associated with nosocomial infections and high mortality rates, representing a serious public health problem.201728764940
evaluation of the rapid polymyxin np test for polymyxin b resistance detection using enterobacter cloacae and enterobacter aerogenes isolates.background: polymyxin resistance is an increasing problem worldwide. currently, determining susceptibility to polymyxins is problematic and lengthy. polymyxins diffuse poorly into agar, potentially giving inaccurate disk diffusion and etest results. a rapid screening test (2 h) for detection of polymyxin-resistance in enterobacteriaceae, developed by nordmann and poirel (rapid polymyxin np test, 2016), detects glucose metabolization in the presence of polymyxins e and b via ph-induced color chan ...201728747375
multidrug resistant bacteria are sensitive to euphorbia prostrata and six others cameroonian medicinal plants extracts.multidrug resistant (mdr) bacteria are responsible for therapeutic failure and there is an urgent need for novels compounds efficient on them.201728743314
formate and nitrate utilization in enterobacter aerogenes for semi-anaerobic production of isobutanol.anaerobic bioprocessing is preferred because of its economic advantages. however, low productivity and decreased growth of the host strain have limited the use of the anaerobic process. anaerobic respiration can be applied to anoxic processing using formate and nitrate metabolism to improve the productivity of value-added metabolites. we constructed isobutanol-producing strains using enterobacter aerogenes as a host strain by metabolic engineering approaches. the byproduct pathway (ldha, buda, a ...201728731532
development of an isothermal amplification-based assay for the rapid visual detection of salmonella bacteria.the efficient and timely detection of pathogens is a major concern worldwide. the aim of this study was to establish a rapid detection method for salmonella bacteria in food samples to facilitate timely treatment. widely used detection methods currently include culture-based methods and pcr-based methods. the former are time consuming, requiring 2 to 3 d, whereas the latter have higher accuracy but are typically complicated, requiring expertise and expensive instruments. in this study, a sensiti ...201728711269
pyrazinone derivatives from the coral-derived aspergillus ochraceus lcj11-102 under high iodide salt.five new pyrazin-2(1h)-one derivatives, ochramides a-d (1-4) and ochralate a (5), as well as three known analogues (6-8) were isolated from the fermentation broth of the marine coral-derived halotolerant aspergillus ochraceus lcj11-102 in a nutrient-limited medium containing 10% nai. their chemical structures were determined by analyzing nmr and x-ray diffraction data. compounds 2, 5 and 6 showed antimicrobial activities against enterobacter aerogenes with the mic values of 40.0, 18.9, and 20.1  ...201728710560
effect of surface roughness in model and fresh fruit systems on microbial inactivation efficacy of cold atmospheric pressure plasma.this study investigates the efficacy of cold atmospheric pressure plasma (capp) on microbial inactivation as influenced by surface roughness of two types of surfaces: sandpaper and fresh fruit peel. different grits of closed-coat sandpaper were selected, with their roughness (pq) values ranging from 6 to 16 μm. apple, orange, and cantaloupe peels were selected for roughness values that were similar to the sandpapers. the sandpapers and the fruit peel surfaces were spot inoculated with enterobact ...201728708029
biosurfactant-enhanced hydrogen production from organic fraction of municipal solid waste using co-culture of e. coli and enterobacter aerogenes.the effect of biosurfactants (surfactin and saponin) on the hydrogen production from organic fraction of municipal solid waste (ofmsw) was investigated using co-culture of facultative anaerobes enterobacter aerogenes and e. coli. the biosurfactants were applied in the concentration ranges of 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 5.0% each. cumulative hydrogen production (p), maximum hydrogen production rate (rmax) and lag phases (λ) were analyzed using modified gompertz model. results revealed t ...201728704737
detection of bacterial sulfatase activity through liquid- and solid-phase colony-based assays.bacterial arylsulfatases are crucial to biosynthesis in many microorganisms, as bacteria often utilize aryl sulfates as a source of sulfur. the bacterial sulfatases are associated with pathogenesis and are applied in many areas such as industry and agriculture. we developed an activity-based probe 1 for detection of bacterial sulfatase activity through liquid- and solid-phase colony-based assays. probe 1 is hydrolyzed by sulfatase to generate fluorescent n-methyl isoindole, which is polymerized ...201728697587
heat resistance of histidine decarboxylase from gram-negative histamine-producing bacteria in seafood.precooking of tuna is a potential critical control point (ccp) in the commercial manufacturing of canned tuna. to assess the efficacy of precooking as a ccp, an understanding of the thermal properties of histamine-producing bacteria (hpb) and their histidine decarboxylase (hdc) enzymes is required. the thermal properties of many hpb have been determined, but the thermal resistances of the hdc enzymes are unknown. the purpose of this study was to determine the d- and z-values of selected hdc enzy ...201728696146
emergence of carbapenem-resistant enterobacteriaceae isolated from patients in a university hospital in saudi arabia. epidemiology, clinical profiles and outcomes.carbapenemase-producing enterobacteriaceae have been steadily spreading worldwide during the last decade. nine patients were identified prospectively and were followed during their hospitalization course to identify the epidemiology, clinical profiles and outcomes. these patients had one or more cultures positive for a cre isolate, contributing to a total of eleven positive cultures from various sites without including duplicates of isolates obtained from the same site. isolates from these patie ...201728642140
detection of new delhi metallo-β-lactamase variants ndm-4, ndm-5, and ndm-7 in enterobacter aerogenes isolated from a neonatal intensive care unit of a north india hospital: a first report.a total 402 enterobacteriaceae isolates were recovered from blood and rectal swabs of 1,000 infants admitted to the neonatal intensive care unit (nicu) of the jawaharlal medical college and hospital aligarh, india. carbapenamase producers were determined by carba np phenotype biochemical assay. out of 402 isolates, it was the first time three of the isolates were identified as enterobacter aerogenes carrying blandm-4, blandm-5, and blandm-7 genes. these genes were identified by polymerase chain ...201728613981
metabolic engineering of enterobacter aerogenes for 2,3-butanediol production from sugarcane bagasse hydrolysate.the pathway engineering of enterobacter aerogenes was attempted to improve its production capability of 2,3-butanediol from lignocellulosic biomass. in the medium containing glucose and xylose mixture as carbon sources, the gene deletion of pflb improved 2,3-butanediol carbon yield by 40%, while the deletion of ptsg increased xylose consumption rate significantly, improving the productivity at 12 hr by 70%. the constructed strain, emy-22-galp, overexpressing glucose transporter (galp) in the tri ...201728596073
colistin and polymyxin b susceptibility testing for carbapenem-resistant and mcr-positive enterobacteriaceae: comparison of sensititre, microscan, vitek 2, and etest with broth microdilution.colistin and polymyxin b remain part of the last line of antibiotics for multi-drug-resistant gram negative bacteria such as carbapenem-resistant-enterobacteriaceae current eucast-clsi joint recommendations are for broth microdilution to be performed for mic testing of colistin. commercial susceptibility testing methods were evaluated and compared against reference broth microdilution (bmd), using a susceptible breakpoint of ≤2mg/l for both colistin and polymyxin b. seventy-six enterobacteriacea ...201728592552
mechanistic insight from calorimetric measurements of the assembly of the binuclear metal active site of glycerophosphodiesterase (gpdq) from enterobacter aerogenes.glycerophosphodiesterase (gpdq) from enterobacter aerogenes is a binuclear metallohydrolase with a high affinity for metal ions at its α site but a lower affinity at its β site in the absence of a substrate. isothermal titration calorimetry (itc) has been used to quantify the co(ii) and mn(ii) binding affinities and thermodynamics of the two sites in wild-type gpdq and two mutants, both in the absence and in the presence of phosphate. metal ions bind to the six-coordinate α site in an entropical ...201728562023
a rapid salmonella detection method involving thermophilic helicase-dependent amplification and a lateral flow assay.salmonella is a major foodborne pathogen that is widespread in the environment and can cause serious human and animal disease. since conventional culture methods to detect salmonella are time-consuming and laborious, rapid and accurate techniques to detect this pathogen are critically important for food safety and diagnosing foodborne illness. in this study, we developed a rapid, simple and portable salmonella detection strategy that combines thermophilic helicase-dependent amplification (thda) ...201728526589
enterobacter aerogenes zdy01 attenuates choline-induced trimethylamine n-oxide levels via remodeling gut microbiota in mice.trimethylamine n-oxide (tmao), which is transformed from trimethylamine (tma) through hepatic flavin-containing monooxygenases, can promote atherosclerosis. tma is produced from dietary carnitine, phosphatidylcholine, and choline via the gut microbes. previous works have shown that some small molecules, such as allicin, resveratrol, and 3,3-dimethyl-1-butanol, are used to reduce circulating tmao levels. however, the use of bacteria as an effective therapy to reduce tmao levels has not been repor ...201728511293
impact of furan derivatives and phenolic compounds on hydrogen production from organic fraction of municipal solid waste using co-culture of enterobacter aerogenes and e. coli.in the present study, the effect of furan derivatives (furfural and 5-hydroxymethylfurfural) and phenolic compounds (vanillin and syringaldehyde) on hydrogen production from organic fraction of municipal solid waste (ofmsw) was investigated using co-culture of facultative anaerobes enterobacter aerogenes and e. coli. the inhibitors were applied in the concentration ranges of 0.25, 0.5, 1, 2 and 5g/l each. inhibition coefficients of phenolic compounds were higher than those of furan derivatives a ...201728500888
antibacterial activities of the methanol extracts of albizia adianthifolia, alchornea laxiflora, laportea ovalifolia and three other cameroonian plants against multi-drug resistant gram-negative bacteria.in the last 10 years, resistance in gram-negative bacteria has been increasing. the present study was designed to evaluate the in vitro antibacterial activities of the methanol extracts of six cameroonian medicinal plants albizia adianthifolia, alchornea laxiflora, boerhavia diffusa, combretum hispidum, laportea ovalifolia and scoparia dulcis against a panel of 15 multidrug resistant gram-negative bacterial strains. the broth microdilution was used to determine the minimal inhibitory concentrati ...201728490970
corrigendum: isolation and characterization of a bacteriophage phieap-2 infecting multidrug resistant enterobacter aerogenes. 201728485399
production of l-lactic acid from metabolically engineered strain of enterobacter aerogenes atcc 29007.in this study, l-lactic acid production was investigated from metabolically engineered strain of e. aerogenes atcc 29007. the engineered strain e. aerogenes sumi01 (δpta) was generated by the deletion of phosphate acetyltransferase (pta) gene from the chromosome of e. aerogenes atcc 29007 and deletion was confirmed by colony pcr. under the optimized fermentation conditions, at 37°c and ph 6 for 84h, the l-lactic acid produced by engineered strain e. aerogenes sumi01 (δpta) in flask fermentation ...201728465055
[effects of vacuum sealing drainage technique in acute and chronic suppurative tenosynovitis of hand].objective: to investigate the effects of vacuum sealing drainage technique in acute and chronic suppurative tenosynovitis of hand. methods: a total of 9 cases acute and chronic suppurative tenosynovitis patients from january 2013 to april 2015 in puai hospital affiliated to tongji medical college, huazhong university of science and technology were retrospectively reviewed. there were 6 males and 3 females, aging from 27 to 65 years, the average age was 55 years. there were 3 cases of index finge ...201728464581
a gene cluster at an unusual chromosomal location responsible for the novel o-antigen synthesis in escherichia coli o62 by the abc transporter-dependent pathway.the o-antigen is a part of the outer membrane of gram-negative bacteria and is related to bacterial virulence. it is one of the most variable cell constituents, and its structural diversity is almost entirely due to genetic variation of the o-antigen gene cluster. in this study, the o-antigen structure of escherichia coli o62 was elucidated by chemical analysis and nuclear magnetic resonance spectroscopy, but showing not consistent with the o-antigen gene cluster between conserved genes galf and ...201728402541
enhanced hydrogen production of enterobacter aerogenes mutated by nuclear irradiation.nuclear irradiation was used for the first time to generate efficient mutants of hydrogen-producing bacteria enterobacter aerogenes, which were screened with larger colour circles of more fermentative acid by-products. e. aerogenes cells were mutated by nuclear irradiation of (60)co γ-rays. the screened e. aerogenes zju1 mutant with larger colour circles enhanced the hydrogenase activity from 89.8 of the wild strain to 157.4mlh2/(gdwh). the hereditary stability of the e. aerogenes zju1 mutant wa ...201728013136
enterobacter aerogenes hormaeche and edwards 1960 (approved lists 1980) and klebsiella mobilis bascomb et al. 1971 (approved lists 1980) share the same nomenclatural type (atcc 13048) on the approved lists and are homotypic synonyms, with consequences for the name klebsiella mobilis bascomb et al. 1971 (approved lists 1980).enterobacter aerogenes hormaeche and edwards 1960 (approved lists 1980) and klebsiella mobilis bascomb et al. 1971 (approved lists 1980) were placed on the approved lists of bacterial names and were based on the same nomenclatural type, atcc 13048. consequently they are to be treated as homotypic synonyms. however, the names of homotypic synonyms at the rank of species normally are based on the same epithet. examination of the rules of the international code of nomenclature of bacteria in force ...201727902205
neonatal sepsis in haiti.infections (including sepsis, meningitis, pneumonia and tetanus) stand as a major contributor to neonatal mortality in haiti (22%). infants acquire bacteria that cause neonatal sepsis directly from the mother's blood, skin or vaginal tract either before or during delivery. nosocomial and environmental pathogens introduce further risk after delivery. the absence of cohesive medical systems and methods for collecting information limits the available data in countries such as haiti. this study seek ...201727765888
optimization of l-asparaginase production from novel enterobacter sp., by submerged fermentation using response surface methodology.the culture conditions and nutritional rations influencing the production of extra cellular antileukemic enzyme by novel enterobacter aerogenes kctc2190/mtcc111 were optimized in shake-flask culture. process variables like ph, temperature, incubation time, carbon and nitrogen sources, inducer concentration, and inoculum size were taken into account. in the present study, finest enzyme activity achieved by traditional one variable at a time method was 7.6 iu/ml which was a 2.6-fold increase compa ...201727340934
detection of new delhi metallo-beta-lactamase (encoded by blandm-1 ) in enterobacter aerogenes in china.the increase in blandm-1 in enterobacteriaceae has become a major concern worldwide. in previous study, we investigated clonal dissemination and mechanisms of resistance to carbapenem in china.201727565452
donor-derived infections among chinese donation after cardiac death liver recipients.to investigate blood cultures of deceased donors and report the confirmed transmission of bacterial infection from donors to liver recipients.201728883707
ramr is not involved in the regulation of rama associated antibiotic resistance in salmonella enterica serovar typhi.rama, a global transcriptional activator, belongs to the arac/xyls family of regulatory proteins and can regulate multidrug resistance through activating the expression of acrab. in salmonella spp., klebsiella pneumonia, enterobacter cloacae and enterobacter aerogenes, ramr represses the transcription of gene rama through binding to the upstream sequences of rama. in this study, we found that the locus and transcription directions of rama-ramr in s. typhi gifu10007 are different from that in s. ...201728882783
pathway engineering of enterobacter aerogenes to improve acetoin production by reducing by-products formation.enterobacter aerogenes was metabolically engineered for acetoin production. to remove the pathway enzymes that catalyzed the formation of by-products, the three genes encoding a lactate dehydrogenase (ldha) and two 2,3-butanediol dehydrogenases (budc, and dhad), respectively, were deleted from the genome. the acetoin production was higher under highly aerobic conditions. however, an extracellular glucose oxidative pathway in e. aerogenes was activated under the aerobic conditions, resulting in t ...201728859805
risk factors for relapse or persistence of bacteraemia caused by enterobacter spp.: a case-control study.enterobacter spp. possess chromosomal ampc beta-lactamases that may be expressed at high levels. previous studies have demonstrated a risk of relapsed bacteraemia following therapy with third generation cephalosporins (3gcs). what additional factors predict microbiological failure in enterobacter bacteraemia is unclear. we aimed to determine factors associated with microbiological failure in enterobacter bacteraemia.201728127422
global assessment of the activity of tigecycline against multidrug-resistant gram-negative pathogens between 2004 and 2014 as part of the tigecycline evaluation and surveillance trial.multidrug-resistant (mdr) gram-negative organisms are a burden on the global health care system. the tigecycline evaluation and surveillance trial (test) is an ongoing global study designed to monitor the in vitro activities of tigecycline and a panel of marketed antimicrobials against a range of clinically significant pathogens. in this study, in vitro data are presented for mdr acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterob ...201728124025
evaluation of wound healing, anti-microbial and antioxidant potential of pongamia pinnata in wistar rats.to investigate wound healing, antimicrobial and antioxidant activity of leaf extract of pongamia pinnata.201728053891
antimicrobial assay and genetic screening of selected freshwater cyanobacteria and identification of a biomolecule dihydro-2h-pyran-2-one derivative.explorations of freshwater cyanobacteria as antimicrobial (bacteria, fungi and methicillin-resistant staphylococcus aureus (mrsa) strains) drug resource using bioassay, nrps (non-ribosomal polypeptide synthetase) and pks (polyketide synthase) genes, as well as in silico approach.201728004519
synthesis and antimicrobial evaluation of ester-linked 1,4-disubstituted 1,2,3-triazoles with a furyl/thienyl moiety.twenty ester-linked 1,4-disubstituted 1,2,3-triazoles having a furyl/thienyl moiety have been synthesized from heteroaryl prop-2-yn-1-yl carboxylate and aromatic azides via a cu(i) catalyzed 1,3-dipolar cycloaddition. all the synthesized compounds were characterized by ftir, [formula: see text]h nmr, [formula: see text]c nmr spectroscopy and hrms. synthesized triazoles were tested in vitro for antimicrobial evaluation against gram-negative bacteria-escherichia coli, enterobacter aerogenes and kl ...201727900513
antimicrobial properties of black grape (vitis vinifera l.) peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds.black grape peel possesses a substantial amount of polyphenolic antimicrobial compounds that can be used for controlling the growth of pathogenic microorganisms. the purpose of this study was to assess antibacterial and antifungal activity of black grape peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds, respectively.201726729960
broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-selex.conventional cell-selex aims to isolate aptamers to a single unique target bacteria species. we propose a method to isolate single-stranded dna aptamers that have broad reactivity to multiple bacterial targets belonging to different genera. the key of the proposed method is that targets of interest are changed sequentially at each selex round. the general scheme was examined using six bacteria from different genera, escherichia coli, enterobacter aerogenes, klebsiella pneumoniae, citrobacter fre ...201728272554
prevalence, antibiotic susceptibility and characterization of antibiotic resistant genes among carbapenem-resistant gram-negative bacilli and yeast in intestinal flora of cancer patients in north lebanon.the emergence and spread of carbapenem-resistant bacteria are a significant clinical and public health concern. the aim of the study is to determine the prevalence of intestinal carriage of carbapenem-resistant bacteria and yeasts in cancer patients under chemotherapy. 41 stool samples collected from cancer patients in nini hospital in tripoli, north lebanon have been analyzed. after isolating yeasts and carbapenem-resistant bacteria, a biochemical identification and antimicrobial susceptibility ...201728216021
prevalence of multidrug resistant uropathogenic bacteria in pediatric patients of a tertiary care hospital in eastern india.today, because systemic infections such as urinary tract infection (uti) affect even pediatric patients, antibiotic resistant bacteria have become a constant clinical challenge. in the present study, a total of 1054 urine samples were collected from pediatric patients over 18 months. from these samples, 510 isolates of pathogenic bacteria were collected using hicrome uti agar. antibiotic sensitivity tests of isolates were performed using the kirby-bauer method. two gram-positive bacteria (entero ...201726617250
high prevalence of non-clonal imipenem-nonsusceptible enterobacter spp. isolates in korea and their association with porin down-regulation.we investigated the prevalence and clonal distribution of imipenem-nonsusceptible enterobacter clinical isolates from hospitals in korea and the contributions of various mechanisms to imipenem nonsusceptibility. the in vitro antimicrobial susceptibility to imipenem of 357 non-duplicated enterobacter isolates obtained from eight geographically distant tertiary care hospitals in korea was evaluated. imipenem-nonsusceptible enterobacter isolates were genotyped. additionally, β-lactamase genes were ...201728336135
molecular epidemiology of carbapenemase-producing enterobacteriaceae in a primary care hospital in japan, 2010-2013.recently, carbapenemase-producing enterobacteriaceae (cpe) have been spreading worldwide and have become a threat in healthcare systems. we investigated the isolation frequency and molecular epidemiological characteristics of cpe isolated from clinical samples collected at a primary care hospital over the four years of 2010-2013 in japan. cpe were detected in 17 (0.34%) of 4875 isolates by the broth microdilution method, sodium mercaptoacetate inhibition test, and modified hodge test using merop ...201728161293
prevalence, risk factors, outcomes, and molecular epidemiology of mcr-1-positive enterobacteriaceae in patients and healthy adults from china: an epidemiological and clinical study.the mcr-1 gene confers transferable colistin resistance. mcr-1-positive enterobacteriaceae (mcrpe) have attracted substantial medical, media, and political attention; however, so far studies have not addressed their clinical impact. herein, we report the prevalence of mcrpe in human infections and carriage, clinical associations of mcr-1-positive escherichia coli (mcrpec) infection, and risk factors for mcrpec carriage.201728139431
in vitro susceptibility to 19 agents other than β-lactams among third-generation cephalosporin-resistant enterobacteriaceae recovered on hospital admission.as part of the multicentre antibiotic therapy optimisation study, mic values of 19 non-β-lactam agents were determined for third-generation cephalosporin-resistant escherichia coli, klebsiella species and enterobacter species (3gcreb) isolates collected in german hospitals.201728108677
clonal spread and accumulation of β-lactam resistance determinants in enterobacter aerogenes and enterobacter cloacae complex isolates from infection and colonization in patients at a public hospital in recife, pernambuco, brazil.enterobacter aerogenes and enterobacter cloacae complex are the two species of this genus most involved in healthcare-associated infections that are esbl and carbapenemase producers. this study characterized, phenotypically and genotypically, 51 isolates of e. aerogenes and e. cloacae complex originating from infection or colonization in patients admitted to a public hospital in recife, pernambuco, brazil, by antimicrobial susceptibility profile, analysis of β-lactamase genes (blatem, blashv, bl ...201727902398
surveillance of omadacycline activity tested against clinical isolates from a global (north america, europe, latin america and asia-western pacific) collection (2010-2011).omadacycline is a broad spectrum aminomethylcycline in late-stage clinical development for acute bacterial skin and skin structure infections and community-acquired pneumonia as both an oral and intravenous, once-daily formulation. in this report omadacycline and comparators were tested against 69,246 non-duplicate bacterial isolates collected prospectively during 2010 and 2011 from medical centers in asia-pacific (11,397 isolates), europe (23,490 isolates), latin america (8,038 isolates) and no ...201728223386
effect of intermittent aeration on microbial diversity in an intermittently aerated ifas reactor treating municipal wastewater: a field study.in the present study, the effect of three intermittent aeration (ia) cycles on treatment performance and microbial diversity was investigated in an integrated fixed film activated sludge (ifas) reactor treating municipal wastewater. the results showed that ia strategies were able to achieve efficient removal of organics and nitrogen ranging between 90 and 95% and 70 and 80%, respectively, however the phosphorus removal was found to be inversely proportional to the duration of aeration off time i ...201728095182
antioxidant, anticholinesterase and antibacterial activities of stachys guyoniana and mentha aquatica.stachys guyoniana noë ex. batt. and mentha aquatica l. are two algerian lamiaceae used in folk medicine.201727927090
a new hexahydrobenzopyran derivative from the gorgonian-derived fungus eutypella sp.a new hexahydrobenzopyran derivative cytosporin l (1), and another eight known compounds were isolated from the gorgonian-derived fungus eutypella sp. collected from the south china sea. the structure of 1 was elucidated by comprehensive spectroscopic data. the absolute configuration of 1 was determined by a chemical reaction and the modified mosher's method. the isolated secondary metabolites were evaluated for their antibacterial activities. compound 1 showed antibacterial activity towards mic ...201728278631
presence, distribution and molecular epidemiology of multi-drug-resistant gram-negative bacilli from medical personnel of intensive care units in tianjin, china, 2007-2015.multi-drug-resistant gram-negative bacteria (mdrgnb) have become an important cause of nosocomial infection in intensive care units (icus).201728268024
quantifying bacterial cross-contamination rates between fresh-cut produce and hands.this study quantifies the cross-contamination rates between fresh-cut produce and hands using a nalidixic acid-resistant nonpathogenic enterobacter aerogenes and cocktails of rifampin-resistant salmonella or escherichia coli o157:h7 strains. volunteers performed the e. aerogenes experiments (n = 20), and one of the authors performed the salmonella and e. coli o157:h7 experiments multiple times (n =15 and n =10, respectively). each participant handled 25 g of fresh-cut carrots, celery, or cantalo ...201728221987
a biological method for in-situ synthesis of hydroxyapatite-coated magnetite nanoparticles using enterobacter aerogenes: characterization and acute toxicity assessments.hydroxyapatite (ha)-coated magnetite nanoparticles (mnps) are being widely investigated for various applications in medical engineering and wastewater treatment. in this work, the mnps were thoroughly coated by bacterial synthesized ha nanoparticles during biomineralization process using enterobacter aerogenes. the resulting bacterial-induced precipitate was then calcined at 600°c and investigated with respect to structural characteristics, particle size and magnetic strength by xrd, ft-ir, sem, ...201728183602
motuporamine derivatives as antimicrobial agents and antibiotic enhancers against resistant gram-negative bacteria.dihydromotuporamine c and its derivatives were evaluated for their in vitro antimicrobial activities and antibiotic enhancement properties against gram-negative bacteria and clinical isolates. the mechanism of action of one of these derivatives, motu-n44, was investigated against enterobacter aerogenes by using fluorescent dyes to evaluate outer-membrane depolarization and permeabilization. its efficiency correlated with inhibition of dye transport, thus suggesting that these molecules inhibit d ...201728098416
optimization of organosolv pretreatment of rice straw for enhanced biohydrogen production using enterobacter aerogenes.ethanol organosolv pretreated rice straw was used to produce biohydrogen using enterobacter aerogenes. the effect of temperature (120-180°c), residence time (30-90min), and ethanol concentration (45-75%v/v) on the hydrogen yield, residual biomass, and lignin recovery was investigated using rsm. in contrast to the residual solid and lignin recovery, no considerable trend could be observed for the changes in the hydrogen yield at different treatment severities. the maximum hydrogen yield of 19.73m ...201728042989
etiology of early onset septicemia among neonates at the university college hospital, ibadan, nigeria.neonatal septicemia remains a major cause of newborn deaths in developing countries. its burden is further compounded by the emergence of multidrug-resistant pathogens, which is related to a lack of antibiotic protocols resulting in unrestricted use of antibiotics. the absence of reliable antibiotic sensitivity testing makes the formulation of antibiotic guidelines and judicious use of antibiotics difficult. this study sought to identify the current bacterial agents associated with early onset s ...201628036314
non-thiolate ligation of nickel by nucleotide-free ureg of klebsiella aerogenes.nickel-dependent ureases are activated by a multiprotein complex that includes the gtpase ureg. prior studies showed that nucleotide-free ureg from klebsiella aerogenes is monomeric and binds one nickel or zinc ion with near-equivalent affinity using an undefined binding site, whereas nucleotide-free ureg from helicobacter pylori selectively binds one zinc ion per dimer via a universally conserved cys-pro-his motif in each protomer. iodoacetamide-treated k. aerogenes ureg was nearly unaffected i ...201628004186
occurrence of blastocystis sp. in water catchments at malay villages and aboriginal settlement during wet and dry seasons in peninsular malaysia.in the tropics, there are too few studies on isolation of blastocystis sp. subtypes from water sources; in addition, there is also an absence of reported studies on the occurrence of blastocystis sp. subtypes in water during different seasons. therefore, this study was aimed to determine the occurrence of blastocystis sp. subtypes in river water and other water sources that drained aboriginal vicinity of highly endemic intestinal parasitic infections during wet and dry seasons. water samples wer ...201627761331
cockroaches (blattodea: blattidae): a reservoir of pathogenic microbes in human-dwelling localities in lahore.this study focuses on isolation of pathogenic bacteria from external and internal surfaces of cockroaches collected from houses and hospitals in lahore. in total, 240 adult cockroaches were collected from houses and hospitals by hand or using sticky traps and food-bait traps. cockroach species were identified, and microbial screening was done for external surfaces and gut tracts of cockroaches. jaccard's index of similarity, bray-curtis' index of dissimilarity, and shannon-wiener's diversity ind ...201627744361
characterization and evaluation of antibacterial and antiproliferative activities of crude protein extracts isolated from the seed of ricinus communis in bangladesh.ricinus communis (euphorbiaceae) has previously been reported to possess analgesic, antihistamine, antioxidant and anti-inflammatory activities. this study was designed for isolation, characterization and evaluation of antibacterial and anti-proliferative activities of r. communis seed protein.201627405609
synthesis, base pairing and structure studies of geranylated rna.natural rnas utilize extensive chemical modifications to diversify their structures and functions. 2-thiouridine geranylation is a special hydrophobic trna modification that has been discovered very recently in several bacteria, such as escherichia coli, enterobacter aerogenes, pseudomonas aeruginosa and salmonella typhimurium the geranylated residues are located in the first anticodon position of trnas specific for lysine, glutamine and glutamic acid. this big hydrophobic terpene functional gro ...201627307604
influence of nanomechanical stress induced by zno nanoparticles of different shapes on the viability of cells.there is growing interest in nanostructures interacting with living organisms. however, there are still no general rules for the design of biocompatible nanodevices. here, we present a step towards understanding the interactions between nanostructures and living cells. we study the influence of nanomechanical stress induced by zinc oxide (zno) nanostructures of different shapes on the viability of both prokaryotic (gram-negative bacteria: escherichia coli and enterobacter aerogenes, and gram-pos ...201627074722
characterization of a novel qepa3 variant in enterobacter aerogenes.five isolates harboring qepa were studied by polymerase chain reaction (pcr) amplification and relevant methods. one was determined to be a novel qepa3 from enterobacter aerogenes, and four involved three qepa1 and one qepa2 determinants from escherichia coli; the qepa3 changed five amino acids. these results characterized genetic structures a, b, c, d, and e.201626947590
evaluation of groundwater quality in a rural community in north central of nigeria.evaluation of water quality of nine boreholes and three open hand-dug wells in a rural community in north central nigeria revealed relative abundance of cations na > k > ca > mg > zn > pb and anions cl(-) > po4(2-) > so4(2-) > no3(-) in the boreholes and cations ca > na > k > mg > pb and anions no3(-)  > po4(2-)  > so4(2-) > cl(-) in the wells. the major contaminants exceeding son and who permissible limits were no3(-) , mg, th, ph and mg, pb, th, ph and do in the wells and boreholes, respective ...201626915740
antibacterial and antiinflammatory properties of bovine colostrum.immunity related disorder is one of the leading causes of disease in the world. oxidative stress and microbial infections play a major role in inflammation-induced diseases. bovine colostrum (bc) contains immunoglobulins and lactoferrins which help in building the immunity and protect against the bacterial proliferation and growth.201626899853
in vitro antibacterial activities of p-toluenesulfonyl-hydrazinothiazoles and hydrazinoselenazoles against multi-drug resistant gram-negative phenotypes.bacterial multidrug resistance (mdr) constitutes a major hurdle in the treatment of infectious diseases worldwide. the present study was designed to evaluate the antibacterial activities of synthetic p-toluenesulfonyl-hydrazinothiazoles against multidrug resistant gram-negative bacteria.201626782344
human gut bacteria are sensitive to melatonin and express endogenous circadian rhythmicity.circadian rhythms are fundamental properties of most eukaryotes, but evidence of biological clocks that drive these rhythms in prokaryotes has been restricted to cyanobacteria. in vertebrates, the gastrointestinal system expresses circadian patterns of gene expression, motility and secretion in vivo and in vitro, and recent studies suggest that the enteric microbiome is regulated by the host's circadian clock. however, it is not clear how the host's clock regulates the microbiome. here, we demon ...201626751389
migration of dictyostelium discoideum to the chemoattractant folic acid.dictyostelium discoideum can be grown axenically in a cultured media or in the presence of a natural food source, such as the bacterium klebsiella aerogenes (ka). here we describe the advantages and methods for growing d. discoideum on a bacterial lawn for several processes studied using this model system. when grown on a bacterial lawn, d. discoideum show positive chemotaxis towards folic acid (fa). while these vegetative cells are highly unpolarized, it has been shown that the signaling and cy ...201627271892
Displaying items 1 - 100 of 3180