Publications

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cryptosporidium fayeri n. sp. (apicomplexa: cryptosporidiidae) from the red kangaroo (macropus rufus).the morphology and infectivity of the oocysts of a new species of cryptosporidium from the faeces of the red kangaroo (macropus rufus) are described. oocysts are structurally indistinguishable from those of cryptosporidium parvum. oocysts of the new species are passed fully sporulated, lack sporocysts, and measure 4.5-5.1 microm (mean=4.9) x 3.8-5.0 microm (mean=4.3 microm) with a length to width ratio 1.02:1.18 (mean 1.14) (n=50). oocysts were not infectious for neonate arc swiss mice. multi-lo ...200818251799
cryptosporidium fayeri: diversity within the gp60 locus of isolates from different marsupial hosts.the highly polymorphic 60 kda glycoprotein (gp60) of cryptosporidium is an important tool for investigating the epidemiology of this parasite. characterization of the gp60 gene has only been performed for 3 of the 20 known cryptosporidium species, and has already enabled sub-typing and source tracking of species with human significance. we have characterised a fourth species, cryptosporidium fayeri, at the gp60 locus using isolates (n=26) from different marsupial hosts to assess the diversity of ...200919027006
identification and differentiation of cryptosporidium species by capillary electrophoresis single-strand conformation polymorphism.cryptosporidium species generally lack distinguishing morphological traits, and consequently, molecular methods are commonly used for parasite identification. various methods for cryptosporidium identification have been proposed, each with their advantages and disadvantages. in this study, we show that capillary electrophoresis coupled with single-strand conformation polymorphism (ce-sscp) is a rapid, simple and cost-effective method for the identification of cryptosporidium species and genotype ...201021087296
wildlife-associated cryptosporidium fayeri in human, australia. 201021122247
identification of novel cryptosporidium genotypes in kangaroos from western australia.a total of 763 faecal samples were collected from western grey kangaroos (macropus fuliginosus) in western australia and screened for the presence of cryptosporidium by pcr at the 18s ribosomal rna (rrna) locus. samples that were positive at the 18s locus were also amplified at the actin locus. the overall prevalence was 9.3% (71/763). at the 18s rrna locus, sequences were obtained for 28 of the 71 positives. sequence analysis identified four species; cryptosporidium fayeri in seven isolates, cr ...201121402448
molecular-based investigation of cryptosporidium and giardia from animals in water catchments in southeastern australia.there has been no large-scale systematic molecular epidemiological investigation of the waterborne protozoans, cryptosporidium or giardia, in southeastern australia. here, we explored, for the first time, the genetic composition of these genera in faecal samples from animals in nine melbourne water reservoir areas, collected over a period of two-years. we employed pcr-based single-strand conformation polymorphism (sscp) and phylogenetic analyses of loci (pssu and pgp60) in the small subunit (ssu ...201223357792
cryptosporidium viatorum n. sp. (apicomplexa: cryptosporidiidae) among travellers returning to great britain from the indian subcontinent, 2007-2011.a novel cryptosporidium genotype was identified, among travellers with gastro-intestinal symptoms returning to great britain from the indian subcontinent, for which we propose the name cryptosporidium viatorum n. sp. the epidemiology of these cases was distinctly different from those with cryptosporidium parvum and cryptosporidium hominis. of the 10 cases identified involving c. viatorum, most were in the first quarter of the year. one occurred in 2007, one in 2008, three in 2010 and five to end ...201222633952
diversity of cryptosporidium in brush-tailed rock-wallabies (petrogale penicillata) managed within a species recovery programme.host-parasite relationships are likely to be impacted by conservation management practices, potentially increasing the susceptibility of wildlife to emerging disease. cryptosporidium, a parasitic protozoan genus comprising host-adapted and host-specific species, was used as an indicator of parasite movement between populations of a threatened marsupial, the brush-tailed rock-wallaby (petrogale penicillata). pcr screening of faecal samples (n = 324) from seven wallaby populations across new south ...201525834789
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