Publications

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taxonomic relationships among the taxa in the candida guilliermondii complex, as revealed by comparative electrophoretic karyotyping.electrophoretic karyotypes of 15 type strains of the taxa in the candida guilliermondii complex including candida fukuyamaensis nakase et al. and candida xestobii yarrow et s. a. meyer were comparatively analysed by using the chef (contour-clamped homogeneous electric field) method of pfge. eighteen strains (isolated from various natural sources in china) which were originally identified as c. guilliermondii by conventional methods were also included. six electrophoretic karyotype groups were re ...200010826830
yeast involved in fermentation of coffea arabica in east africa determined by genotyping and by direct denaturating gradient gel electrophoresis.samples of coffea arabica were collected during the different stages of the fermentation from two production sites in tanzania. the yeasts community was identified by genotyping using its-pcr and sequence analysis of the d1/d2 domain of the 26s rrna gene. for confirmation, denaturating gradient gel electrophoresis (dgge) of pcr-amplified 26s rrna gene was performed to detect yeast directly from coffee samples without cultivation. yeast counts were in the range 4.0 x 10(4) - 5.0 x 10(7) cfu/g wit ...200415164358
two new species in the pichia guilliermondii clade: pichia caribbica sp. nov., the ascosporic state of candida fermentati, and candida carpophila comb. nov.pichia caribbica sp. nov. (type strain dbvpg 4519, nrrl y-27274, cbs 9966) is described as the ascosporic state of candida fermentati, and candida guilliermondii var. carpophila (type strain dbvpg 7739, nrrl y-17905, cbs 5256) is elevated to species status as candida carpophila comb. nov. these new taxa, which are indistinguishable on the basis of conventional taxonomic criteria, differ from one another and from pichia guilliermondii by low dna base sequence relatedness, different electrophoreti ...200515691751
responses of candida fukuyamaensis rcl-3 and rhodotorula mucilaginosa rcl-11 to copper stress.the effect of high cu(ii) concentrations on superoxide dismutase (sod) and catalase (cat) activity in candida fukuyamaensis rcl-3 and rhodotorula mucilaginosa rcl-11, previously isolated from a copper filter at a mine plant in argentina, was studied. addition of 0.1, 0.2 and 0.5 mm cu(ii) to the culture medium increased total sod and cat activity in both strains. native polyacrylamide gel electrophoresis revealed two bands with sod activity for c. fukuyamaensis rcl-3 and only one for r. mucilagi ...200919322830
yeast diversity of ghanaian cocoa bean heap fermentations.the fermentation of the theobroma cacao beans, involving yeasts, lactic acid bacteria, and acetic acid bacteria, has a major influence on the quality of the resulting cocoa. an assessment of the microbial community of cocoa bean heap fermentations in ghana resulted in 91 yeast isolates. these were grouped by pcr-fingerprinting with the primer m13. representative isolates were identified using the d1/d2 region of the large subunit rrna gene, internal transcribed spacer sequences and partial actin ...200919473277
biodegradation and mineralization of metolachlor and alachlor by candida xestobii.metolachlor (2-chloro-6'-ethyl-n-(2-methoxy-1-methylethyl)aceto-o-toluidide) is a pre-emergent chloroacetanilide herbicide used to control broadleaf and annual grassy weeds in a variety of crops. the s enantiomer, s-metolachlor, is the most effective form for weed control. although the degradation of metolachlor in soils is thought to occur primarily by microbial activity, little is known about the microorganisms that carry out this process and the mechanisms by which this occurs. this study exa ...201021190381
the environmental and intrinsic yeast diversity of cuban cocoa bean heap fermentations.the environmental yeast diversity of spontaneous cocoa bean fermentations in east cuba was investigated. seven fermentations, 25 equipment- and handling-related samples, and 115 environmental samples, such as flowers, leaf and cocoa pod surfaces, as well as drosophilid insects, were analysed. the basic fermentation parameters temperature and ph were recorded during five fermentations for at least six days. a total of 435 yeast isolates were identified by a combination of pcr-fingerprinting of ge ...201627322722
disentangling metabolic pathways involved in copper resistance in candida fukuyamaensis rcl-3 indigenous yeast.candida fukuyamaensis rcl-3 yeast strain isolated from a copper filter plant is able to lower copper concentration in culture medium. in the present study, effect of copper in proteins expression and mechanisms involved in copper resistance were explored using comparative proteomics. mono-dimensional gel electrophoresis revealed differential band expressions between cells grown with or without copper. 2-de analysis of c. fukuyamaensis rcl-3 revealed that copper exposure produced at least an over ...201626568043
[biomineralization of copper in candida fukuyamaensis rcl-3].candida fukuyamaensis rcl-3 yeast has the ability to decrease copper concentration in a culture medium. high copper concentrations change the cell color from white/cream to brown. the effect of color change ceases with the addition of kcn or when cells are grown in a culture medium without sulfate ions. these results could be associated with cus bioaccumulation in the cell surface. this report revealed that mineralization would be a mechanism used by this yeast for copper bioremediation.201727237424
association between grape yeast communities and the vineyard ecosystems.the grape yeast biota from several wine-producing areas, with distinct soil types and grapevine training systems, was assessed on five islands of azores archipelago, and differences in yeast communities composition associated with the geographic origin of the grapes were explored. fifty-seven grape samples belonging to the vitis vinifera grapevine cultivars verdelho dos açores (verdelho), arinto da terceira (arinto) and terrantez do pico (terrantez) were collected in two consecutive years and 40 ...201728085916
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