Publications

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effects of kinetin and related compounds on growth and sexual reproduction of saprolegnia australis.an isolate of saprolegnia australis grew readily and produced abundant sex organs on a chemically defined medium. the optimal temperature for growth was higher (25°) than the optimal temperature for sexual reproduction (20°). addition of the purines, adenine and hypoxanthine to the medium stimulated production of oogonia while addition of kinetin and 6-benzylaminopurine inhibited this process. when kinetin and adenine were added together, the two purines interacted in affecting oogonial producti ...196724522507
re-evaluation of the enigmatic species complex saprolegnia diclina-saprolegnia parasitica based on morphological, physiological and molecular data.the phylogenetic relationships among isolates of the saprolegnia diclina-saprolegnia parasitica complex were investigated based on its rdna sequences, and correlated with morphological and physiological characters. the isolates studied belong to five phylogenetically separate clades. the majority of presumed parasitic isolates, mostly isolated from fish lesions, fell within a clade that comprises isolates which has been variously named as s. diclina type 1, s. parasitica, saprolegnia salmonis or ...200717446097
ulcerative disease outbreak in crayfish orconectes propinquus linked to saprolegnia australis in big muskellunge lake, wisconsin.crayfish populations in the area of the north temperate lakes long term ecological research (lter) project, wisconsin, usa, have been monitored for >25 yr. in 2005, native crayfish orconectes propinquus from big muskellunge lake were found with ulcerated lesions in the cuticle. in 2006, lesions occurred in 9.5% of sampled crayfish from the lake (n=3146). ulcers generally occurred on the appendages of affected individuals but varied in location and severity. the prevalence of ulcers varied widely ...201020853742
the diversity of oomycetes on crayfish: morphological vs. molecular identification of cultures obtained while isolating the crayfish plague pathogen.numerous oomycetes colonise the crayfish cuticle, the best known being the crayfish plague pathogen aphanomyces astaci. although other oomycetes associated with crayfish complicate the isolation and molecular detection of a. astaci, their diversity is little known. to improve this knowledge, we analysed 95 oomycete isolates obtained during attempts to isolate a. astaci from crayfish presumably infected by this pathogen. we characterized the isolates morphologically and by sequencing of the nucle ...201324119406
reprint of: the diversity of oomycetes on crayfish: morphological vs. molecular identification of cultures obtained while isolating the crayfish plague pathogen.numerous oomycetes colonise the crayfish cuticle, the best known being the crayfish plague pathogen aphanomyces astaci. although other oomycetes associated with crayfish complicate the isolation and molecular detection of a. astaci, their diversity is little known. to improve this knowledge, we analysed 95 oomycete isolates obtained during attempts to isolate a. astaci from crayfish presumably infected by this pathogen. we characterized the isolates morphologically and by sequencing of the nucle ...201425088074
molecular identification of a bronopol tolerant strain of saprolegnia australis causing egg and fry mortality in farmed brown trout, salmo trutta.some species of the genus saprolegnia, such as saprolegnia diclina and saprolegnia ferax are responsible for devastating infections on salmonid eggs. members of this group cause saprolegniasis, a disease resulting in considerable economic losses in aquaculture. although both s. diclina and s. ferax have received much attention, the role of other saprolegnia species in infecting fish eggs is less known. for this purpose, we have investigated the aetiology of chronic egg mortality events occurring ...201425088073
topographical mapping of the rainbow trout (oncorhynchus mykiss) microbiome reveals a diverse bacterial community with antifungal properties in the skin.the mucosal surfaces of wild and farmed aquatic vertebrates face the threat of many aquatic pathogens, including fungi. these surfaces are colonized by diverse symbiotic bacterial communities that may contribute to fight infection. whereas the gut microbiome of teleosts has been extensively studied using pyrosequencing, this tool has rarely been employed to study the compositions of the bacterial communities present on other teleost mucosal surfaces. here we provide a topographical map of the mu ...201526209676
synthesis and evaluation of novel oxyalkylated derivatives of 2',4'-dihydroxychalcone as anti-oomycete agents against bronopol resistant strains of saprolegnia sp.a series of novel oxyalkylchalcones substituted with alkyl groups were designed and synthesized, and the antioomycete activity of the series was evaluated in vitro against saprolegnia strains. all tested o-alkylchalcones were synthesized by means of nucleophilic substitution from the natural compound 2',4'-dihydroxychalcone (1) and the respective alkyl bromide. the natural chalcone (1) and 10 synthetic oxyalkylchalcones (2-11) were tested against saprolegnia parasitica and saprolegnia australis. ...201627556457
hemi-synthesis and anti-oomycete activity of analogues of isocordoin.an efficient synthesis of a series of 4'-oxyalkyl-isocordoin analogues (2-8) is reported for the first time. their structures were confirmed by ¹h-nmr, (13)c-nmr, and hrms. their anti-oomycete activity was evaluated by mycelium and spores inhibition assay against two selected pathogenic oomycetes strains: saprolegnia parasitica and saprolegnia australis. the entire series of isocordoin derivatives (except compound 7) showed high inhibitory activity against these oomycete strains. among them, com ...201728604594
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