Publications

structure of an archaeal virus capsid protein reveals a common ancestry to eukaryotic and bacterial viruses.	archaea and their viruses are poorly understood when compared with the eukarya and bacteria domains of life. we report here the crystal structure of the major capsid protein (mcp) of the sulfolobus turreted icosahedral virus, an archaeal virus isolated from an acidic hot spring (ph 2-4, 72-92 degrees c) in yellowstone national park. the structure is nearly identical to the mcp structures of the eukaryotic paramecium bursaria chlorella virus, and the bacteriophage prd1, and shows a common fold wi ...	2005	16357204
characterization of the archaeal thermophile sulfolobus turreted icosahedral virus validates an evolutionary link among double-stranded dna viruses from all domains of life.	icosahedral nontailed double-stranded dna (dsdna) viruses are present in all three domains of life, leading to speculation about a common viral ancestor that predates the divergence of eukarya, bacteria, and archaea. this suggestion is supported by the shared general architecture of this group of viruses and the common fold of their major capsid protein. however, limited information on the diversity and replication of archaeal viruses, in general, has hampered further analysis. sulfolobus turret ...	2006	16840341
structure of a197 from sulfolobus turreted icosahedral virus: a crenarchaeal viral glycosyltransferase exhibiting the gt-a fold.	sulfolobus turreted icosahedral virus (stiv) was the first icosahedral virus characterized from an archaeal host. it infects sulfolobus species that thrive in the acidic hot springs (ph 2.9 to 3.9 and 72 to 92 degrees c) of yellowstone national park. the overall capsid architecture and the structure of its major capsid protein are very similar to those of the bacteriophage prd1 and eukaryotic viruses paramecium bursaria chlorella virus 1 and adenovirus, suggesting a viral lineage that predates t ...	2006	16840342
crystal structure of afv3-109, a highly conserved protein from crenarchaeal viruses.	the extraordinary morphologies of viruses infecting hyperthermophilic archaea clearly distinguish them from bacterial and eukaryotic viruses. moreover, their genomes code for proteins that to a large extend have no related sequences in the extent databases. however, a small pool of genes is shared by overlapping subsets of these viruses, and the most conserved gene, exemplified by the orf109 of the acidianus filamentous virus 3, afv3, is present on genomes of members of three viral familes, the ...	2007	17241456
a new dna binding protein highly conserved in diverse crenarchaeal viruses.	sulfolobus turreted icosahedral virus (stiv) infects sulfolobus species found in the hot springs of yellowstone national park. its 37 open reading frames (orfs) generally lack sequence similarity to other genes. one exception, however, is orf b116. while its function is unknown, orthologs are found in three additional crenarchaeal viral families. due to the central importance of this protein family to crenarchaeal viruses, we have undertaken structural and biochemical studies of b116. the struct ...	2007	17336360
a winged-helix protein from sulfolobus turreted icosahedral virus points toward stabilizing disulfide bonds in the intracellular proteins of a hyperthermophilic virus.	sulfolobus turreted icosahedral virus (stiv) was the first non-tailed icosahedral virus to be isolated from an archaeal host. like other archaeal viruses, its 37 open reading frames generally lack sequence similarity to genes with known function. the roles of the gene products in this and other archaeal viruses are thus largely unknown. however, a protein's three-dimensional structure may provide functional and evolutionary insight in cases of minimal sequence similarity. in this vein, the struc ...	2007	17669459
archaeal proviruses tkv4 and mvv extend the prd1-adenovirus lineage to the phylum euryarchaeota.	the viral lineage hypothesis predicting a common origin for viruses that infect hosts residing in different domains of life gains more support as data on viral structures accumulates. one such lineage is the prd1-adenovirus lineage, which unites icosahedral dsdna viruses with large facets and a double beta-barrel trimer coat protein. this lineage is represented by a number of viruses infecting bacteria and eukaryotes. however, only one member of the lineage, sulfolobus turreted icosahedral virus ...	2008	18308362
transcriptome analysis of infection of the archaeon sulfolobus solfataricus with sulfolobus turreted icosahedral virus.	microarray analysis of infection by sulfolobus turreted icosahedral virus (stiv) revealed insights into the timing and extent of virus transcription, as well as differential regulation of host genes. using a microarray containing genes from both the host and the virus, the infection cycle of stiv was studied. following infection of sulfolobus solfataricus strain 2-2-12 with stiv, transcription of virus genes was first detected at 8 h postinfection (p.i.), with a peak at 24 h p.i. lysis of cells ...	2008	18337566
genetics, biochemistry and structure of the archaeal virus stiv.	stiv (sulfolobus turreted icosahedral virus) has been the subject of detailed structural, genetic, transcriptomic, proteomic and biochemical studies. stiv arguably has been investigated in more detail than any other archaeal virus. as a result, we know more about stiv than other viruses infecting members of the archaea domain. like most viruses isolated from crenarchaeal hosts, stiv has little in common with viruses that infect eukaryotic and bacterial hosts and should be considered the founding ...	2009	19143613
particle assembly and ultrastructural features associated with replication of the lytic archaeal virus sulfolobus turreted icosahedral virus.	little is known about the replication cycle of archaeal viruses. we have investigated the ultrastructural changes of sulfolobus solfataricus p2 associated with infection by sulfolobus turreted icosahedral virus (stiv). a time course of a near synchronous stiv infection was analyzed using both scanning and transmission electron microscopy. assembly of stiv particles, including particles lacking dna, was observed within cells, and fully assembled stiv particles were visible by 30 h postinfection ( ...	2009	19357174
familial relationships in hyperthermo- and acidophilic archaeal viruses.	archaea often live in extreme, harsh environments such as acidic hot springs and hypersaline waters. to date, only two icosahedrally symmetric, membrane-containing archaeal viruses, sh1 and sulfolobus turreted icosahedral virus (stiv), have been described in detail. we report the sequence and three-dimensional structure of a third such virus isolated from a hyperthermoacidophilic crenarchaeon, sulfolobus strain g4st-2. characterization of this new isolate revealed it to be similar to stiv on the ...	2010	20164227
the architecture and chemical stability of the archaeal sulfolobus turreted icosahedral virus.	viruses utilize a diverse array of mechanisms to deliver their genomes into hosts. while great strides have been made in understanding the genome delivery of eukaryotic and prokaryotic viruses, little is known about archaeal virus genome delivery and the associated particle changes. the sulfolobus turreted icosahedral virus (stiv) is a double-stranded dna (dsdna) archaeal virus that contains a host-derived membrane sandwiched between the genome and the proteinaceous capsid shell. using cryo-elec ...	2010	20592081
in vivo assembly of an archaeal virus studied with whole-cell electron cryotomography.	we applied whole-cell electron cryotomography to the archaeon sulfolobus infected by sulfolobus turreted icosahedral virus (stiv), which belongs to the prd1-adeno lineage of dsdna viruses. stiv infection induced the formation of pyramid-like protrusions with sharply defined facets on the cell surface. they had a thicker cross-section than the cytoplasmic membrane and did not contain an exterior surface protein layer (s-layer). intrapyramidal bodies often occupied the volume of the pyramids. matu ...	2010	21134637
potential role of cellular escrt proteins in the stiv life cycle.	we are examining the archaeal virus stiv (sulfolobus turreted icosahedral virus) in order to elucidate the details of its replication cycle and its interactions with its cellular host, sulfolobus solfataricus. infection of sulfolobus by stiv initiates an unusual cell lysis pathway. one component of this pathway is the formation of pyramid-like structures on the surface of infected cells. multiple seven-sided pyramid-like structures are formed on infected cells late in the stiv replication cycle. ...	2011	21265756
in vivo virus structures: simultaneous classification, resolution enhancement, and noise reduction in whole-cell electron tomography.	sulfolobus turreted icosahedral virus (stiv) experiences an extra-cellular environment of near boiling acid (80-¦c, ph 3) and particles purified under these conditions were previously analyzed by cryo electron microscopy and image reconstruction. here we describe cryo-tomograms of solfolobus cells infected with stiv and the maximum likelihood algorithm employed to compute reconstructions of virions within the cell. virions in four different tomograms were independently reconstructed with an aver ...	2011	21396453
development of a genetic system for the archaeal virus sulfolobus turreted icosahedral virus (stiv).	our understanding of archaeal viruses has been limited by the lack of genetic systems for examining viral function. we describe the construction of an infectious clone for the archaeal virus sulfolobus turreted icosahedral virus (stiv). stiv was isolated from a high temperature (82°c) acidic (ph 2.2) hot spring in yellowstone national park and replicates in the archaeal model organism sulfolobus solfataricus (rice et al., 2004). while stiv is one of most studied archaeal viruses, little is known ...	2011	21496857
sulfolobus turreted icosahedral virus c92 protein responsible for formation of pyramid-like cellular lysis structures.	host cells infected by sulfolobus turreted icosahedral virus (stiv) have been shown to produce unusual pyramid-like structures on their cell surface. these structures represent a viral-induced lysis mechanism that is present in archaea and appears to be distinct from the holin/endolysin system described for dna bacteriophages. this study investigated the stiv gene products required for pyramid formation in its host sulfolobus solfataricus. over-expression of stiv orf c92 in s. solfataricus alone ...	2011	21525343
exceptional virion release mechanism: one more surprise from archaeal viruses.	virion release from the host cell is the final and essential step for completion of the viral life cycle and spread of virions in the environment. although for eukaryotic and bacterial viruses the egress mechanisms are reasonably well understood, this subject has not been studied in detail for archaeal viruses until recently. here we summarize available data on the extraordinary egress mechanism exploited by the sulfolobus islandicus rod-shaped virus sirv2 and the sulfolobus turreted icosahedral ...	2011	21531608