Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| begomoviruses from mungbeans in pakistan: epitope profiles, dna a sequences and phylogenetic relationships. | monoclonal antibodies raised against particles of african cassava mosaic virus, indian cassava mosaic virus or okra leaf curl virus were used to test samples collected in pakistan from begomovirus-infected plants. epitope profiles obtained from cucurbits resembled those previously reported for pakistani begomoviruses. in contrast, epitope profiles obtained from legumes showed little diversity and were quite distinct from these. dna with nucleotide sequences typical of begomovirus dna a component ... | 2004 | 15045567 |
| molecular characterization of two soybean-infecting begomoviruses from india and evidence for recombination among legume-infecting begomoviruses from south [corrected] south-east asia. | the complete nucleotide sequences of two soybean-infecting begomoviruses have been determined from central and southern parts of india. sequence analyses show that the isolate from central india is a strain of mungbean yellow mosaic india virus (mymiv) and the southern indian isolate is a strain of mungbean yellow mosaic virus (mymv). multiple dna b components could be detected with the soybean strain of mungbean yellow mosaic virus species. the nucleotide sequence similarity between the dna a c ... | 2005 | 15681067 |
| absence of interaction of genomic components and complementation between mungbean yellow mosaic india virus isolates in cowpea. | agroinoculations were performed with dna a and dna b components of mungbean yellow mosaic india virus (mymiv) isolates differing in their infectivity on cowpea. exchange of genomic components of the mymiv isolates occurred in all the leguminous species but not in cowpea. extremely low viral dna accumulation and atypical leaf curl symptoms produced by reassortants in cowpea suggest barriers both for replication and systemic movement despite genetic similarity. | 2005 | 15931466 |
| cloning, restriction mapping and phylogenetic relationship of genomic components of mymiv from lablab purpureus. | the present work describes cloning of genomic components of whitefly transmitted geminivirus infecting lablab purpureus syn. dolichos lablab (commonly known as dolichos bean or hyacinth bean). the genome characterization using pcr with geminiviral degenerate primers and dna sequencing were used to describe the bipartite virus associated with yellow mosaic disease of dolichos lablab. full-length dna-a and dna-b clones were obtained. the dna-a sequence analysis showed that the isolate was similar ... | 2006 | 16242317 |
| studies on the activity of a bidirectional promoter of mungbean yellow mosaic india virus by agroinfiltration. | the av promoter expressing av1 and av2 genes and ac1 promoter expressing ac1 gene are present in opposite orientation in the intergenic region of mungbean yellow mosaic india virus (mymiv). transient agrobacterium-mediated delivery of putative promoter constructs into nicotiana benthamiana and different legumes, followed by reporter gene (beta-d-glucuronidase, gus) assay, identified the promoter region of both ac1 and av genes that is necessary for transcriptional initiation. transcription activ ... | 2006 | 16458986 |
| the oligomeric rep protein of mungbean yellow mosaic india virus (mymiv) is a likely replicative helicase. | geminiviruses replicate by rolling circle mode of replication (rcr) and the viral rep protein initiates rcr by the site-specific nicking at a conserved nonamer (taatatt downward arrow ac) sequence. the mechanism of subsequent steps of the replication process, e.g. helicase activity to drive fork-elongation, etc. has largely remained obscure. here we show that rep of a geminivirus, namely, mungbean yellow mosaic india virus (mymiv), acts as a replicative helicase. the rep-helicase, requiring > or ... | 2006 | 17142233 |
| new motifs within the nb-arc domain of r proteins: probable mechanisms of integration of geminiviral signatures within the host species of fabaceae family and implications in conferring disease resistance. | the gemini viruses are a group of plant infectious agents, of which mungbean yellow mosaic india virus (mymiv) belongs to the bipartite subgroup of gemini virus and causes serious yield penalty in the leguminous group of plants. in this investigation we have isolated two resistant gene homologues (rghs; ay301990, ay301991) from two mymiv-resistant lines of vigna mungo and v. radiata that have high homology with a mymiv-resistant linked marker, vmyr1 (ay 297425). these three resistance factors al ... | 2007 | 17320114 |
| intervention of geminiviral replication in yeast by ribozyme mediated downregulation of its rep protein. | geminiviruses pose serious threat to many economically important crops such as mungbean, tomato, cotton, etc. to devise a specific antiviral strategy at the viral dna replication level, a hammerhead ribozyme was directed against the mrna of the replication initiator protein (rep). rep is the most important viral protein for the dna replication of the mungbean yellow mosaic india virus (mymiv), a member of the geminiviridae family. the ribozyme showed approximately 33% cleavage activity on synthe ... | 2007 | 17531225 |
| legume yellow mosaic viruses: genetically isolated begomoviruses. | summary: the yellow mosaic diseases of a number of legumes across southern asia are caused by four species of whitefly-transmitted geminiviruses (genus begomovirus, family geminiviridae): mungbean yellow mosaic virus, mungbean yellow mosaic india virus, dolichos yellow mosaic virus and horsegram yellow mosaic virus. they cause losses to a number of important pulse crops, a major source of dietary protein in the region. the viruses have host ranges limited to plants of the family fabaceae and eff ... | 2007 | 20507504 |
| dna recognition properties of the cell-to-cell movement protein (mp) of soybean isolate of mungbean yellow mosaic india virus (mymiv-sb). | bipartite geminiviruses possess two movement proteins (nsp and mp), which mediate the intra- and intercellular movement. in order to accomplish the transport process the movement proteins interact with viral nucleic acids in a sequence non-specific manner. to investigate the nucleic acid recognition properties of mp of mymiv-sb, the protein was expressed in escherichia coli as a fusion protein with maltose-binding protein (mbp) and purified in native condition. gel mobility shift assay was emplo ... | 2008 | 17949843 |
| cowpea golden mosaic disease in gujarat is caused by a mungbean yellow mosaic india virus isolate with a dna b variant. | it has long been assumed that cowpea golden mosaic disease (cgmd) in southern asia is caused by a begomovirus distinct from those causing disease in other legumes. the components of a begomovirus causing cgmd in western india were isolated, cloned and sequenced. analysis of the sequences shows the virus to be an isolate of mungbean yellow mosaic india virus, but with a distinct dna b component with greater similarity to components of a second legume-infecting begomovirus occurring in the region, ... | 2008 | 18516489 |
| mymiv replication initiator protein (rep): roles at the initiation and elongation steps of mymiv dna replication. | in order to explore the mechanism of geminivirus dna replication, we show that the replication initiator (rep) protein encoded by mungbean yellow mosaic india virus (mymiv), a member of the family geminiviridae, binds specifically to the iterons present in the viral dna replication origin (cr-a) in a highly ordered manner that might be a prerequisite for the initiation of replication. mymiv rep also acts as a helicase during the post-initiation stage and is upregulated in presence of the rpa32 s ... | 2008 | 18703212 |
| soybean cultivar resistant to mungbean yellow mosaic india virus infection induces viral rna degradation earlier than the susceptible cultivar. | yellow mosaic disease caused by whitefly-transmitted bipartite geminiviruses is one of the major constraints on productivity of a number of pulse crops. we have cloned the bipartite genome of mungbean yellow mosaic india virus isolated from infected soybean. we report here that agroinfection of soybean seedlings with a single uncut recombinant binary plasmid containing tandem dimers of both dna a and dna b resulted in 100% infectivity in susceptible varieties. to understand the mechanism of natu ... | 2009 | 19394372 |
| molecular characterisation and infectivity of a "legumovirus" (genus begomovirus: family geminiviridae) infecting the leguminous weed rhynchosia minima in pakistan. | the legume yellow mosaic viruses (lymvs) that cause extensive losses to grain legumes across southern asia are an evolutionarily unusual group of begomoviruses (genus begomovirus; family geminiviridae) with bipartite genomes. all previously identified lymvs were isolated from leguminous crop species. here we have identified a virus related to the lymvs in a common weed, the legume rhynchosia minima originating from pakistan. analysis of the sequence of the virus shows it to be a typical bipartit ... | 2009 | 19664665 |
| two new 'legumoviruses' (genus begomovirus) naturally infecting soybean in nigeria. | two new 'legumoviruses' (genus begomovirus; family geminiviridae) naturally infecting soybean (glycine max l. merr.) in nigeria were molecularly characterized. based on characteristic symptoms in soybean, the two viruses are provisionally designated as soybean mild mottle virus (sbmmv) and soybean chlorotic blotch virus (sbcbv). sbcbv has a bipartite genome, whereas sbmmv has only a dna a component. the dna a component of sbmmv is 2,768 nucleotides (nt) long and the dna a and dna b components of ... | 2010 | 20229118 |
| genetic diversity and phylogeography of begomoviruses infecting legumes in pakistan. | grain legumes are an important source of dietary protein across southern asia, but they suffer extensive losses due to several viruses that are members of the genus begomovirus (family geminiviridae), which are collectively known as legume yellow mosaic viruses (lymvs). despite their economic importance, little attention has been paid to lymvs in pakistan and only partial sequences of virus isolates originating from this country are available in the databases. here, a survey of lymvs occurring a ... | 2010 | 20375225 |
| proteomic analysis of salicylic acid induced resistance to mungbean yellow mosaic india virus in vigna mungo. | the role of salicylic acid (sa) in inducing resistance to mymiv infection in vigna mungo has been elucidated by proteomics. twenty-nine proteins identified by maldi-tof/tof, predicted to be involved in stress responses, metabolism, photosynthesis, transport and signal transduction, showed increased abundance upon sa treatment. susceptible plants showed characteristic yellow mosaic symptoms upon mymiv infection. a concentration dependent decrease in physiological symptoms associated with mymiv wa ... | 2010 | 21130907 |
| engineering resistance against mungbean yellow mosaic india virus using antisense rna. | yellow mosaic disease of cultivated legumes in south-east asia, is caused by mungbean yellow mosaic india virus (mymiv) and mungbean yellow mosaic virus (mymv) belonging to the genus begomovirus of the family geminiviridae. efforts to engineer resistance against the genus begomovirus are focused mainly on silencing of complementary-sense virus genes involved in virus replication. here we have targeted a complementary-sense gene (aci) encoding replication initiation protein (rep) to develop resis ... | 2010 | 23637483 |
| molecular marker-assisted genotyping of mungbean yellow mosaic india virus resistant germplasms of mungbean and urdbean. | mungbean yellow mosaic india virus (mymiv) belonging to the genus begomovirus causes the yellow mosaic disease in a number of economically important edible grain legumes including mungbean (vigna radiata), urdbean (vigna mungo) and soybean (glycine max). the disease is severe, critical, open spread and inflicts heavy yield losses annually. the objective of this study is to develop molecular markers linked to mymiv-resistance to facilitate genotyping of urdbean and mungbean germplasms for mymiv-r ... | 2011 | 20652447 |
| infectivity analysis of a blackgram isolate of mungbean yellow mosaic virus and genetic assortment with mymiv in selective hosts. | yellow mosaic disease in grain legumes in indian subcontinent is caused by two important virus species viz. mungbean yellow mosaic virus (mymv) and mungbean yellow mosaic india virus (mymiv), belonging to the genus begomovirus of the family geminiviridae. the genomic components of a begomovirus causing yellow mosaic disease in blackgram in southern india were cloned and sequenced. nucleotide sequence comparison of dna a component shows the virus isolate to be a variant of mungbean yellow mosaic ... | 2011 | 21380756 |
| velvet bean severe mosaic virus: a distinct begomovirus species causing severe mosaic in mucuna pruriens (l.) dc. | velvet bean [mucuna pruriens (l.) dc] is one of the most important medicinal plants. it is used to treat many ailments, but is widely used for the treatment especially for parkinson's disease because of the presence of 3,4-dihydroxyphenylalanine (l: -dopa) in it. it was noticed in last 5 years that the plants in the field showed severe mosaic, downward curling of the leaves, stunting, etc. this is consistently observed over the years in india. the disease was transmitted by whiteflies and by gra ... | 2011 | 21556742 |
| mutagenesis in orf av2 affects viral replication in mungbean yellow mosaic india virus. | mungbean yellow mosaic india virus (mymiv) is a whitefly-transmitted begomovirus with a bipartite genome. we investigate the functions of the mymiv-av2 protein, the open reading frame present upstream of the coat protein gene in dna a component. the ability of mymiv-av2 mutants to replicate, spread and cause symptoms in legume hosts, blackgram, cowpea and french bean was analysed. plants agroinoculated with mutants k73r, c86s and the double mutant c84s,c86s showed increase in severity of symptom ... | 2011 | 21654086 |
| enhanced viral intergenic region-specific short interfering rna accumulation and dna methylation correlates with resistance against a geminivirus. | rna silencing mediated by short-interfering rna (sirna) is used by plants as a defense against viruses. in the case of geminiviruses, viral dna is targeted at the transcriptional level, while virus-derived transcripts are targeted by posttranscriptional silencing. mungbean yellow mosaic india virus (mymiv), a bipartite geminivirus, causes yellow mosaic disease in soybean (glycine max). a soybean variety resistant to this disease has been identified (line pk416). to understand the molecular mecha ... | 2011 | 21692636 |
| Enhanced nicking activity of Rep in presence of pre-coat protein of Mungbean yellow mosaic India virus. | Yellow mosaic disease causes severe yield loss in grain legumes in Indian subcontinent and south east Asia. The disease is caused by two virus species, Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV). They have genome organization typical of Old World begomoviruses, the unique feature being the presence of an open reading frame (ORF) AV2 upstream of coat protein gene. In order to elucidate its function, ORF AV2 of blackgram isolate, Mungbean yellow mosaic India ... | 2011 | 22173984 |
| isolation, characterization, and structure analysis of a non-tir-nbs-lrr encoding candidate gene from mymiv-resistant vigna mungo. | yellow mosaic disease of vigna mungo caused by mungbean yellow mosaic india virus (mymiv) is still a major threat in the crop production. a candidate disease resistance (r) gene, cyr1 that co-segregates with mymiv-resistant populations of v. mungo has been isolated. cyr1 coded in silico translated protein sequence comprised of 1,176 amino acids with coiled coil structure at the n-terminus, central nucleotide binding site (nbs) and c-terminal leucine-rich repeats (lrr) that belongs to non-tir-nbs ... | 2011 | 22205242 |
| A novel role for RAD54: this host protein modulates geminiviral DNA replication. | Geminiviruses primarily encode only few factors, such as replication initiator protein (Rep), and need various host cellular machineries for rolling-circle replication (RCR) and/or recombination-dependent replication (RDR). We have identified a host factor, RAD54, in a screen for Rep-interacting partners and observed its role in DNA replication of the geminivirus mungbean yellow mosaic India virus (MYMIV). We identified the interacting domains ScRAD54 and MYMIV-Rep and observed that ScRAD54 enha ... | 2011 | 22171001 |
| mymiv-ac2, a geminiviral rnai suppressor protein, has potential to increase the transgene expression. | gene silencing is one of the limiting factors for transgene expression in plants. but the plant viruses have learnt to suppress gene silencing by encoding the protein(s), called rna silencing suppressor(s) (rss). hence, these proteins could be used to overcome the limitation for transgene expression. the rnai suppressors, namely hc-pro and p19, have been shown to enhance the transgene expression but other rss proteins have not been screened for similar role. moreover, none of rsss from the dna v ... | 2012 | 22592775 |
| spectral reflectance pattern in soybean for assessing yellow mosaic disease. | remote sensing technique is useful for monitoring large crop area at a single time point, which is otherwise not possible by visual observation alone. yellow mosaic disease (ymd) is a serious constraint in soybean production in india. however, hardly any basic information is available for monitoring ymd by remote sensing. present study examines spectral reflectance of soybean leaves due to mungbean yellow mosaic india virus (mymiv) infection in order to identify ymd sensitive spectral ratio or r ... | 2013 | 24426282 |
| detection of quantitative trait loci for mungbean yellow mosaic india virus (mymiv) resistance in mungbean (vigna radiata (l.) wilczek) in india and pakistan. | yellow mosaic disease (ymd) is one of the major diseases affecting mungbean (vigna radiata (l.) wilczek). in this study, we report the mapping of the quantitative trait locus (qtl) for mungbean yellow mosaic india virus (mymiv) resistance in mungbean. an f8 recombinant inbred line (ril) mapping population was generated in thailand from a cross between nm10-12-1 (mymiv resistance) and kps2 (mymiv susceptible). one hundred and twenty-two rils and their parents were evaluated for mymiv resistance i ... | 2013 | 24399908 |
| proteomics approach combined with biochemical attributes to elucidate compatible and incompatible plant-virus interactions between vigna mungo and mungbean yellow mosaic india virus. | vigna mungo, a tropical leguminous plant, highly susceptible to yellow mosaic disease caused by mungbean yellow mosaic india virus (mymiv) resulting in high yield penalty. the molecular events occurring during compatible and incompatible interactions between v. mungo and mymiv pathosystem are yet to be explored. in this study biochemical analyses in conjunction with proteomics of mymiv-susceptible and -resistant v. mungo genotypes were executed to get an insight in the molecular events during co ... | 2013 | 23587433 |
| the host factor rad51 is involved in mungbean yellow mosaic india virus (mymiv) dna replication. | geminiviruses replicate their single-stranded genomes with the help of only a few viral factors and various host cellular proteins primarily by rolling-circle replication (rcr) and/or recombination-dependent replication. atrad51 has been identified, using the phage display technique, as a host factor that potentially interacts with the rep protein of mungbean yellow mosaic india virus (mymiv), a member of the genus begomovirus. in this study, we demonstrate the interaction between mymiv rep and ... | 2013 | 23575883 |
| arabidopsis thaliana mcm2 plays role(s) in mungbean yellow mosaic india virus (mymiv) dna replication. | geminiviruses are plant pathogens with single-stranded (ss) dna genomes of about 2.7 kb in size. they replicate primarily via rolling-circle replication (rcr) with the help of a few virally encoded factors and various host-cell machineries. the virally encoded replication initiator protein (rep) is essential for geminivirus replication. in this study, by interaction screening of an arabidopsis thaliana cdna library, we have identified a host factor, mcm2, that interacts with the rep protein of t ... | 2013 | 23242774 |
| differential soybean gene expression during early phase of infection with mungbean yellow mosaic india virus. | mungbean yellow mosaic india virus (mymiv), a bipartite begomovirus, causes yellow mosaic disease to soybean. studies related to host gene expression in response to begomovirus infection have mostly been performed with systemically infected tissues at a later period of infection. in this study, soybean gene expression analysis has been performed to understand local responses against mymiv at an early stage of infection before appearance of detectable limit of late viral transcripts. 444 soybean ... | 2014 | 24752408 |
| unfolding of in planta activity of anti-rep ribozyme in presence of a rna silencing suppressor. | antisense rna ribozymes have intrinsic endonucleolytic activity to effect cleavage of the target rna. however, this activity in vivo is often controlled by the dominance of antisense or other double-stranded rna mechanism. in this work, we demonstrate the in planta activity of a hammerhead ribozyme designed to target rep-mrna of a phytopathogen mungbean yellow mosaic india virus (mymiv) as an antiviral agent. we also found rna-silencing is induced on introduction of catalytically active as well ... | 2014 | 24735726 |
| arabidopsis thaliana nac083 protein interacts with mungbean yellow mosaic india virus (mymiv) rep protein. | geminiviral replication initiator protein (rep) is a key player in geminiviral rolling circle mode of replication. however, the virus exploits various host cellular machineries for its replication. study of these host factors is important to understand the geminiviral dna replication in greater details. with this view, we screened for the peptides interacting with the rep protein of a representative of geminivirus, namely, mungbean yellow mosaic india virus (mymiv), employing phage display techn ... | 2014 | 24442717 |
| mungbean yellow mosaic indian virus encoded ac2 protein suppresses rna silencing by inhibiting arabidopsis rdr6 and ago1 activities. | rna silencing refers to a conserved rna-directed gene regulatory mechanism in a wide range of eukaryotes. it plays an important role in many processes including growth, development, genome stability, and antiviral defense in the plants. geminivirus encoded ac2 is identified as an rna silencing suppressor protein, however, the mechanism of action has not been characterized. in this paper, we elucidate another mechanism of ac2-mediated suppression activity of mungbean yellow mosaic india virus (my ... | 2015 | 26433748 |
| the ac5 protein encoded by mungbean yellow mosaic india virus is a pathogenicity determinant that suppresses rna silencing-based antiviral defenses. | it is generally accepted that begomoviruses in the family geminiviridae encode four proteins (from ac1/c1 to ac4/c4) using the complementary-sense dna as template. although ac5/c5 coding sequences are increasingly annotated in databases for many begomoviruses, the evolutionary relationships and functions of this putative protein in viral infection are obscure. here, we demonstrate several important functions of the ac5 protein of a bipartite begomovirus, mungbean yellow mosaic india virus (mymiv ... | 2015 | 26010321 |
| transcript dynamics at early stages of molecular interactions of mymiv with resistant and susceptible genotypes of the leguminous host, vigna mungo. | initial phases of the mymiv-vigna mungo interaction is crucial in determining the infection phenotype upon challenging with the virus. during incompatible interaction, the plant deploys multiple stratagems that include extensive transcriptional alterations defying the virulence factors of the pathogen. such molecular events are not frequently addressed by genomic tools. in order to obtain a critical insight to unravel how v. mungo respond to mungbean yellow mosaic india virus (mymiv), we have em ... | 2015 | 25884711 |
| genome-wide snp identification and characterization in two soybean cultivars with contrasting mungbean yellow mosaic india virus disease resistance traits. | mungbean yellow mosaic india virus (mymiv) is a bipartite geminivirus, which causes severe yield loss in soybean (glycine max). considering this, the present study was conducted to develop large-scale genome-wide single nucleotide polymorphism (snp) markers and identify potential markers linked with known disease resistance loci for their effective use in genomics-assisted breeding to impart durable mymiv tolerance. the whole-genome re-sequencing of mymiv resistant cultivar 'upsm-534' and suscep ... | 2015 | 25875830 |
| rad54 is not essential for any geminiviral replication mode in planta. | the circular single-stranded dna of phytopathogenic geminiviruses is propagated by three modes: complementary strand replication (csr), rolling circle replication (rcr) and recombination-dependent replication (rdr), which need host plant factors to be carried out. in addition to necessary host polymerases, proteins of the homologous recombination repair pathway may be considered essential, since geminiviruses are particularly prone to recombination. among several others, rad54 was suggested to b ... | 2015 | 25492528 |
| molecular and biochemical characterization of a vigna mungo map kinase associated with mungbean yellow mosaic india virus infection and deciphering its role in restricting the virus multiplication. | yellow mosaic disease caused by the begomovirus mungbean yellow mosaic india virus (mymiv) severely affects many economically important legumes. recent investigations in vigna mungo - mymiv incompatible interaction identified a mapk homolog in the defense signaling pathway. an important branch of immunity involves phosphorylation by evolutionary conserved mitogen-activated protein kinases (mapk) that transduce signals of pathogen invasion to downstream molecules leading to diverse immune respons ... | 2017 | 28716408 |
| linkage mapping of mungbean yellow mosaic india virus (mymiv) resistance gene in soybean. | mungbean yellow mosaic india virus (mymiv) is one of the most prevalent pathogen that limits soybean production in india. in this study rils derived from js335, dominant but mymiv susceptible variety and pi171443, donor of mymiv resistance gene in most of the mymiv resistant varieties released in india and f2 population derived from sl525, a resistant variety released for northern india and nrc101, a susceptible genotype were used to study the inheritance of mymiv resistance and map the gene res ... | 2017 | 28588385 |
| high throughput sequencing reveals modulation of micrornas in vigna mungo upon mungbean yellow mosaic india virus inoculation highlighting stress regulation. | micrornas (mirnas) are 20-24 nucleotides long non-coding rnas known to play important regulatory roles during biotic and abiotic stresses by controlling gene expression. blackgram (vigna mungo), an economically important grain legume is highly susceptible to pathogenic begomovirus mungbean yellow mosaic india virus (mymiv) and resulting in high yield loss. in this study two different leaf-small-rna libraries were prepared from the pooled rna at three different time points of resistant v. mungo i ... | 2017 | 28224923 |