Publications

single-stranded dna genome in a whitefly-transmitted plant virus.	bean golden yellow mosaic virus (gymv) dna sedimented in sucrose density gradients and in an analytical ultracentrifuge as a single component. values of s(20,w) which depended upon ionic strength (i) and ph, were 16.0 (ph = 7.0, i = 0.1); 16.3 (ph = 7.0, i = 1.0); 9.0 (ph = 13, i = 0.1); and 12.0 (0.1 n naoh, i = 1.0). gymv-dna banded in cscl as a single component with a buoyant density of 1.7170 g/ml and exhibited hyperchromicity when heated between 20 and 70 degrees . hyperchromicity and a shi ...	1977	18625485
bean dwarf mosaic virus bv1 protein is a determinant of the hypersensitive response and avirulence in phaseolus vulgaris.	the capacities of the begomoviruses bean dwarf mosaic virus (bdmv) and bean golden yellow mosaic virus (bgymv) to differebean dwarf mosaic viru certain common bean (phaseolus vulgaris) cultivars were used to identify viral determinants of the hypersensitive response (hr) and avirulence (avr) in bdmv. a series of hybrid dna-b components, containing bdmv and bgymv sequences, was constructed and coinoculated with bdmv dna-a (bdmv-a) or bdmva-green florescent protein into seedlings of cv. topcrop (s ...	2000	11059485
bean golden yellow mosaic virus from chiapas, mexico: characterization, pseudorecombination with other bean-infecting geminiviruses and germ plasm screening.	abstract the complete nucleotide (nt) sequences of the cloned dna-a (2644 nts) and dna-b (2609 nts) components of bean golden yellow mosaic virus (bgymv-mx) from chiapas, mexico were determined. the genome organization of bgymv-mx is similar to that of other western hemisphere bipartite geminiviruses (genus begomovirus). infectivity of the cloned bgymv-mx dna components in common bean (phaseolus vulgaris) plants was demonstrated by particle bombardment and agroinoculation. bgymv-mx was identifie ...	2000	18944424
identifying resistance gene analogs associated with resistances to different pathogens in common bean.	abstract a polymerase chain reaction approach using degenerate primers that targeted the conserved domains of cloned plant disease resistance genes (r genes) was used to isolate a set of 15 resistance gene analogs (rgas) from common bean (phaseolus vulgaris). eight different classes of rgas were obtained from nucleotide binding site (nbs)-based primers and seven from not previously described toll/interleukin-1 receptor-like (tir)-based primers. putative amino acid sequences of rgas were signific ...	2003	18944161
two newly described begomoviruses of macroptilium lathyroides and common bean.	abstract macroptilium lathyroides, a perennial weed in the caribbean region and central america, is a host of macroptilium yellow mosaic florida virus (maymfv) and macroptilium mosaic puerto rico virus (mamprv). the genomes of maymfv and mamprv were cloned from m. lathyroides and/or field-infected bean and the dna sequences were determined. cloned a and b components for both viruses were infectious when inoculated to m. lathyroides and common bean. comparison of the dna sequences for cloned a an ...	2003	18943157
a genetic linkage map of phaseolus vulgaris l. and localization of genes for specific resistance to six races of anthracnose (colletotrichum lindemuthianum).	a genetic map of common bean was constructed using 197 markers including 152 rapds, 32 rflps, 12 scars, and 1 morphological marker. the map was established by using a f(2) population of 85 individuals from the cross between a line derived from the spanish landrace andecha (andean origin) and the mesoamerican genotype a252. the resulting map covers about 1,401.9 cm, with an average marker distance of 7.1 cm and includes molecular markers linked to disease resistance genes for anthracnose, bean co ...	2007	17186216
genetic mapping of the bean golden yellow mosaic geminivirus resistance gene bgm-1 and linkage with potyvirus resistance in common bean (phaseolus vulgaris l.).	bean golden yellow mosaic virus (bgymv) is a whitefly-transmitted geminivirus of the begomovirus family that causes important yield losses to common beans grown in tropical and sub-tropical countries of latin america and the caribbean. a major resistance gene that has been widely deployed in this region is the recessive locus bgm-1 that prevents the development of severe yellowing typical of the disease. in this study, we developed a co-dominant sequence-characterized amplified region (scar) mar ...	2007	17115130
the n-terminus of the begomovirus nuclear shuttle protein (bv1) determines virulence or avirulence in phaseolus vulgaris.	the bv1 gene of the bipartite begomovirus genome encodes a nuclear shuttle protein (nsp) that is also an avirulence determinant in common bean. the function of the nsp of two common bean-infecting bipartite begomoviruses, bean dwarf mosaic virus (bdmv) and bean golden yellow mosaic virus (bgymv), was investigated using a series of hybrid dna-b components expressing chimeric bdmv and bgymv nsp, and genotypes of the two major common bean gene pools: andean (cv. topcrop) and middle american (cvs. a ...	2007	17990960
preliminary identification and coat protein gene phylogenetic relationships of begomoviruses associated with native flora and cultivated plants from the yucatan peninsula of mexico.	a number of native and cultivated eudicots in the yucatan peninsula of mexico (ypm) exhibit symptoms associated with virus infection. symptomatic leaves were collected and assessed for begomoviral detection using polymerase chain reaction (pcr), and universal primers that amplify a fragment of the coat protein gene (core cp). begomovirus were detected in nine native and seven cultivated species, representing seven eudicot families. dna extracts from the 16 hosts were used for pcr amplification a ...	2007	17682933
inoculation of plants with begomoviruses by particle bombardment without cloning: using rolling circle amplification of total dna from infected plants and whiteflies.	a new system for inoculation of plants with begomoviral dna without cloning or the use insect vectors is described. total dna extracted from begomovirus-infected plants was amplified by rolling circle amplification (rca) using the bacteriophage phi29 dna polymerase, and inoculated to plants by particle bombardment. infection rates of up to 100% were obtained using this technique. this technique successfully inoculated all the begomoviruses evaluated: five bipartite (bean golden yellow mosaic vir ...	2010	20447420
screening metagenomic data for viruses using the e-probe diagnostic nucleic acid assay.	next generation sequencing (ngs) is not used commonly in diagnostics, in part due to the large amount of time and computational power needed to identify the taxonomic origin of each sequence in a ngs data set. by using the unassembled ngs data sets as the target for searches, pathogen-specific sequences, termed e-probes, could be used as queries to enable detection of specific viruses or organisms in plant sample metagenomes. this method, designated e-probe diagnostic nucleic acid assay, first t ...	2014	25207481
evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics.	plant viruses in the genus begomovirus, family geminiviridae often cause substantial crop losses. these viruses have been emerging in many locations throughout the tropics and subtropics. like many plant viruses, they are often not recognized by plant diagnostic clinics due in large part to the lack of rapid and cost effective assays. an isothermal amplification assay, recombinase polymerase amplification (rpa), was evaluated for its ability to detect three begomoviruses and for its suitability ...	2016	27000806
simultaneous detection of both rna and dna viruses infecting dry bean and occurrence of mixed infections by bgymv, bcmv and bcmnv in the central-west region of mexico.	a multiplex reverse transcription polymerase chain reaction (rt-pcr) assay was developed to simultaneously detect bean common mosaic virus (bcmv), bean common mosaic necrotic virus (bcmnv), and bean golden yellow mosaic virus (bgymv) from common bean leaves dried with silica gel using a single total nucleic acid extraction cetyl trimethyl ammonium bromide (ctab) method. a mixture of five specific primers was used to amplify three distinct fragments corresponding to 272 bp from the ac1 gene of bg ...	2017	28358318