Publications

vibrio tasmaniensis sp. nov., isolated from atlantic salmon (salmo salar l.).	we describe the polyphasic characterization of four vibrio isolates which formed a tight aflp group in a former study. the group was closely related to v. cyclitrophicus, v. lentus and v. splendidus (98.2-98.9% similarity) on the basis of the 16s rdna sequence analysis, but by dna-dna hybridisation experiments it had at maximum 61% dna similarity towards v. splendidus. thus, we propose that the isolates represent a new vibrio species i.e. v. tasmaniensis (lmg 20012t; embl under the accession num ...	2003	12747411
concurrence of cat and tet genes in multiple antibiotic-resistant bacteria isolated from a sea cucumber and sea urchin mariculture farm in china.	a basic understanding of abundance and diversity of antibiotic-resistant microbes and their genetic determinants is necessary for finding a way to prevent and control the spread of antibiotic resistance. for this purpose, chloramphenicol and multiple antibiotic-resistant bacteria were screened from a mariculture farm in northern china. both sea cucumber and sea urchin rearing ponds were populated with abundant antibiotic-resistant bacteria, especially marine vibrios. sixty-five percent chloramph ...	2006	16909348
differential effects of temperature and starvation on induction of the viable-but-nonculturable state in the coral pathogens vibrio shiloi and vibrio tasmaniensis.	we compared induction of the viable-but-nonculturable (vbnc) state in two vibrio spp. isolated from diseased corals by starving the cells and maintaining them in artificial seawater at 4 and 20 degrees c. in vibrio tasmaniensis, isolated from a gorgonian octocoral growing in cool temperate water (7 to 17 degrees c), the vbnc state was not induced by incubation at 4 degrees c after 157 days. by contrast, vibrio shiloi, isolated from a coral in warmer water (16 to 30 degrees c), was induced into t ...	2006	17021199
diseases affect cold-water corals too: eunicella verrucosa (cnidaria: gorgonacea) necrosis in sw england.	the first recorded incidence of cold-water coral disease was noted in eunicella verrucosa, a coral on the international 'red list' of threatened species, at a marine protected area in sw england in 2002. video surveys of 634 separate colonies at 13 sites revealed that disease outbreaks were widespread in sw england from 2003 to 2006. coenchyme became necrotic in diseased specimens, leading to tissue sloughing and exposing skeletal gorgonin to settlement by fouling organisms. sites where necrosis ...	2007	17760382
defences against oxidative stress in vibrios associated with corals.	bacteria colonizing healthy coral tissue may produce enzymes capable of overcoming the toxic effects of reactive oxygen species, including superoxide dismutase (sod) and catalase. significant differences in the activities of these enzymes were observed in cultures of vibrio campbellii, vibrio coralliilyticus, vibrio harveyi, vibrio mediterranei, vibrio pelagius, vibrio rotiferanus, vibrio tasmaniensis, and photobacterium eurosenbergii isolated from healthy, bleached or necrotic tropical and cold ...	2008	18279336
fast detection of vibrio species potentially pathogenic for mollusc.	vibrio tasmaniensis, vibrio splendidus and vibrio neptunius species were distributed worldwide and associated with aquaculture and have been reported as the cause of diseases in aquatic organisms. polyphasic analyses for bacterial identification are not feasible for routine diagnostic because of the time involved. the aim of this study is to design three pcr primer sets that can assist with fast detection of these species. they were designed from the 16s ribosomal rna gene, and pcr conditions we ...	2009	19640660
effect of intestinal autochthonous probiotics isolated from the gut of sea cucumber (apostichopus japonicus) on immune response and growth of a. japonicus.	the study isolated 224 bacteria from the intestine of apostichopus japonicus, then selected and identified three of the bacteria (hs1, hs7, and hs10) which demonstrated amylase, lipase, and protease production capacity as candidate probiotics for sea cucumbers. the three potential probiotics showed no pathogenicity both in hemolytic assays on sheep blood agar plates and after immersing sea cucumbers in a suspension of the bacteria. to reveal the effects of these three potential probiotics on the ...	2014	24727198
outer membrane vesicles are vehicles for the delivery of vibrio tasmaniensis virulence factors to oyster immune cells.	vibrio tasmaniensis lgp32, a facultative intracellular pathogen of oyster haemocytes, was shown here to release outer membrane vesicles (omvs) both in the extracellular milieu and inside haemocytes. intracellular release of omvs occurred inside phagosomes of intact haemocytes having phagocytosed few vibrios as well as in damaged haemocytes containing large vacuoles heavily loaded with lgp32. the omv proteome of lgp32 was shown to be rich in hydrolases (25%) including potential virulence factors ...	2015	24919412
exposure to the paralytic shellfish toxin producer alexandrium catenella increases the susceptibility of the oyster crassostrea gigas to pathogenic vibrios.	the multifactorial etiology of massive crassostrea gigas summer mortalities results from complex interactions between oysters, opportunistic pathogens and environmental factors. in a field survey conducted in 2014 in the mediterranean thau lagoon (france), we evidenced that the development of the toxic dinoflagellate alexandrium catenella, which produces paralytic shellfish toxins (psts), was concomitant with the accumulation of psts in oyster flesh and the occurrence of c. gigas mortalities. in ...	2016	26784228
distinct immune responses of juvenile and adult oysters (crassostrea gigas) to viral and bacterial infections.	since 2008, massive mortality events of pacific oysters (crassostrea gigas) have been reported worldwide and these disease events are often associated with ostreid herpesvirus type 1 (oshv-1). epidemiological field studies have also reported oyster age and other pathogens of the vibrio genus are contributing factors to this syndrome. we undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult c. gigas at different time-p ...	2016	27439510
copper homeostasis at the host vibrio interface: lessons from intracellular vibrio transcriptomics.	recent studies revealed that several vibrio species have evolved the capacity to survive inside host cells. however, it is still often ignored if intracellular stages are required for pathogenicity. virulence of vibrio tasmaniensis lgp32, a strain pathogenic for crassostrea gigas oysters, depends on entry into hemocytes, the oyster immune cells. we investigated here the mechanisms of lgp32 intracellular survival and their consequences on the host-pathogen interaction. entry and survival inside h ...	2016	26472275
environmental vibrios represent a source of antagonistic compounds that inhibit pathogenic vibrio cholerae and vibrio parahaemolyticus strains.	with the overuse of antibiotics, many pathogens including vibrio cholerae and vibrio parahaemolyticus have evolved multidrug resistance making treatment more difficult. while understanding the mechanisms that underlie pathogenesis is crucial, knowledge of bacterial interactions of v. cholerae and v. parahaemolyticus could provide insight to their susceptibility outside of the human host. based on previous work showing competition among environmental strains, we predict that marine-derived bacter ...	2017	28857444
characterization of n-acyl homoserine lactones in vibrio tasmaniensis lgp32 by a biosensor-based uhplc-hrms/ms method.	since the discovery of quorum sensing (qs) in the 1970s, many studies have demonstrated that vibrio species coordinate activities such as biofilm formation, virulence, pathogenesis, and bioluminescence, through a large group of molecules called n-acyl homoserine lactones (ahls). however, despite the extensive knowledge on the involved molecules and the biological processes controlled by qs in a few selected vibrio strains, less is known about the overall diversity of ahls produced by a broader r ...	2017	28425948