Publications

in vitro expression of a full-length dna copy of cowpea mosaic virus b rna: identification of the b rna encoded 24-kd protein as a viral protease.	double-stranded cdna was synthesized from b component rna of cowpea mosaic virus and cloned into appropriate vectors. using four clones, together representing the entire b rna sequence, a full-length dna copy was constructed and subsequently positioned downstream of a phage sp6 or t7 promoter. rna molecules transcribed from this full-size dna copy using sp6 or t7 rna polymerase were efficiently translated in rabbit reticulocyte lysates into a 200-kd polypeptide similar to rna isolated from viral ...	1987	16453750
translation initiation potential of the 5' proximal augs of the polycistronic p/c mrna of sendai virus. a multipurpose vector for site-specific mutagenesis.	to determine the translation initiation potential of the 5' proximal four augs of the polycistronic p/c mrna of sendai virus, we have developed an efficient system for oligonucleotide-directed site-specific mutagenesis utilizing a plasmid vector which contains the replication origin of the single-stranded dna phage f1 and the phage sp6 promoter. utilizing uridine containing single-stranded dna templates of the plasmid vector carrying the p/c gene we introduced point mutations in all four of the ...	1988	2832411
evidence that sindbis virus nsp2 is an autoprotease which processes the virus nonstructural polyprotein.	the four nonstructural proteins (nsp1-4) of sindbis virus, a member of the togaviridae family, are initially expressed from the 5' segment of the single-stranded genomic (+)rna as a polyprotein which is subsequently proteolytically processed. in attempts to identify the protease acting on this nonstructural polyprotein, we established a coupled in polyprotein, we established a coupled in vitro transcription-translation system which was able to faithfully process the major polyprotein when an mrn ...	1989	2525839