Publications

genetic heterogeneity of phthorimaea operculella granulovirus: restriction analysis of wild-type isolates and clones obtained in vitro.	genetic heterogeneity of a wild-type granulovirus (tunisia isolate) of the potato tuber moth phthorimaea operculella (phthorimaea operculella granulovirus, phop gv) has been studied. the heterogeneity was indicated by the presence of several submolar fragments in the profiles obtained by use of several restriction endonucleases. it was also demonstrated by variations in the restriction profile of the wild-type tunisia isolate that had underwent since 1991 in our laboratory numerous passages in v ...	1998	9645238
detection of phthorimaea operculella granulovirus in vivo and in vitro by a specific dna probe.	in analyzing populations of non-infected potato tuber moth (ptm) phthorimaea operculella, using a total dna probe from phthorimaea operculella granulovirus (phopgv), false positive reactions were obtained indicating homology between cellular and viral dnas. using a cloned 2.1 kbp fragment of phopgv dna, a specific digoxigenin-labeled probe was developed. this fragment did not show homology using both dot and southern blot hybridization with either the genome of the larvae or genomes of the cell ...	1999	10757228
choristoneura fumiferana granulovirus p74 protein, a highly conserved baculoviral envelope protein.	a gene that encodes a homologue to baculoviral p74, an envelope-associated viral structural protein, has been identified and sequenced on the genome of choristoneura fumiferana granulovirus (chfugv). a part of the chfugv p74 gene was located on an 8.9 kb bamhi subgenomic fragment using different sets of degenerated primers. these were designed using the results of the protein sequencing of a major 74 kda structural protein that is associated with the occlusion-derived virus (odv). the gene has a ...	2003	14536031
identification, characterization and phylogenic analysis of conserved genes within the odvp-6e/odv-e56 gene region of choristoneura fumiferana granulovirus.	the genes that are located within the odvp-6e/odv-e56 region of the choristoneura fumiferana granulovirus (chfugv) were identified by sequencing the 11 kb bamhi restriction fragment on the chfugv genome. the global gc content that was calculated from the data obtained from this genomic region was 34.96%. the open-reading frames (orfs), located within the odvp-6e/odv-e56 region, are presented and compared to the equivalent orfs that are located at the same region in other gvs. this region is comp ...	2004	15469697
identification, characterization and phylogenic analysis of conserved genes within the p74 gene region of choristoneura fumiferana granulovirus genome.	the genes located within the p74 gene region of the choristoneura fumiferana granulovirus (chfugv) were identified by sequencing an 8.9 kb bamhi restriction fragment on the chfugv genome. the global guanine-cytosine (gc) content of this region of the genome was 33.02%. this paper presents the orfs within the p74 gene region along with their transcriptional orientations. this region contains a total of 15 open reading frames (orfs). among those, 8 orfs were found to be homologues to the baculovir ...	2004	15607029
establishment and characterization of spodoptera littoralis cell line (sl 96), adapted at 19 degrees c permissive for different baculoviruses.	cells from ovarioule tissues of the egyptian cotton leaf worm, spodoptera littoralis (sl 96) were established, identified and characterized comparing with other lepidopteran cell lines using isozymes, karyotyping, rapd pcr, and polyacrylamide gel electrophoresis polypeptide page profile. comparison of the number of chromosomes and page profiles; also the isozymes profiles demonstrate the possibility to distinguish the cell lines by those analyses and having a distinguishable finger print for eve ...	2005	16333885
genetic and biological analysis of colombian phthorimaea operculella granulovirus isolated from tecia solanivora (lepidoptera: gelechiidae).	tecia solanivora (lepidoptera: gelechiidae) is an invasive potato pest of the north of south america that recently colonized zones where phthorimaea operculella (lepidoptera: gelechiidae), a taxonomically related insect, was established. nowadays, both species can be found in most areas in different proportions. the phthorimaea operculella granulovirus (phopgv) was found to efficiently control p. operculella and was used as a biopesticide in storage conditions. however, no appropriate biological ...	2010	20870793
experimental mixtures of phthorimaea operculella granulovirus isolates provide high biological efficacy on both phthorimaea operculella and tecia solanivora (lepidoptera: gelechiidae).	the guatemalan potato moth tecia solanivora (povolny) recently invaded part of south america, colonizing zones where phthorimaea operculella (zeller), another potato moth species belonging to the same group, was previously established. t. solanivora is now the major insect pest of potato in this area encompassing venezuela, colombia and ecuador. p. operculella granulovirus (phopgv) (betabaculovirus) is a biocontrol agent to be considered for the simultaneous management of these two potato pests, ...	2012	22575733
development of a viral biopesticide for the control of the guatemala potato tuber moth tecia solanivora.	the guatemala potato tuber moth tecia solanivora (povolny) (lep. gelechiidae) is an invasive species from mesoamerica that has considerably extended its distribution area in recent decades. while this species is considered to be a major potato pest in venezuela, colombia, and ecuador, currently no specific control methods are available for farmers. to address this issue we developed a biopesticide formulation to be used in integrated pest management of t. solanivora, following three steps. first ...	2013	23232473
betabaculovirus f proteins showed different efficiencies when rescuing the infectivity of gp64-null autographa californica nucleopolyhedrovirus.	the agrotis segetum granulovirus (agsegv) f protein was previously identified as the first betabaculovirus f protein with functional homology to autographa californica nucleopolyhedrovirus (acmnpv) gp64. in the current study, f proteins from xestia c-nigrum granulovirus (xecngv), cydia pomonella granulovirus (cpgv), phthorimaea operculella granulovirus (phopgv), choristoneura occidentalis granulovirus (chocgv) and plutella xylostella gv (plxygv) were studied for their ability to rescue the infec ...	2013	23245471
the isolation and genetic characterisation of a south african strain of phthorimaea operculella granulovirus, phopgv-sa.	the phthorimaea operculella granulovirus (phopgv) is considered a promising biopesticide that can be incorporated into integrated pest management programmes for sustainable control of the potato tuber moth, phthorimaea operculella (zeller) (lepidoptera: gelechiidae), a major pest of solanaceous crops in sub-tropical and tropical regions worldwide. several phopgv isolates recovered from geographically different insect populations have been genetically characterised, and the full genome of the tun ...	2014	24503224
the comparative analysis of complete genome sequences from two south african betabaculoviruses: phthorimaea operculella granulovirus and plutella xylostella granulovirus.	the complete genomes of two novel south african betabaculovirus isolates, namely phthorimaea operculella granulovirus (phopgv-sa) and plutella xylostella granulovirus (plxygv-sa), were sequenced and compared to the respective reference isolates phopgv-1346 and plxygv-k1. for both isolates, the genome size and guanine-cytosine (gc) content were similar to those of the respective reference genomes. however, numerous-single nucleotide polymorphisms (snps) and several insertions/deletions were obser ...	2016	27456426