Publications

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pedobacter cryoconitis sp. nov., a facultative psychrophile from alpine glacier cryoconite.on the basis of phenotypic and genotypic characteristics, a novel species belonging to the genus pedobacter is described. a facultatively psychrophilic, gram-negative, aerobic, rod-shaped strain, a37(t), was isolated from alpine glacier cryoconite. the non-flagellated and non-spore-forming isolate grew over a temperature range of 1-25 degrees c, showed activities of oxidase, catalase, dnase, protease (gelatin, casein), amylase, beta-glucosidase, beta-galactosidase and beta-lactamase and degraded ...200313130009
a cold-active extracellular metalloprotease from pedobacter cryoconitis--production and properties.an extracellular protease from pedobacter cryoconitis, isolated from alpine cryoconite on glacier ice, was purified and characterized. despite high cell densities at a temperature range of 1-25 degrees c, the optimum temperature for protease production was 15 degrees c. maximum enzyme production was achieved when the strain was grown in a ph-neutral medium containing soybean meal, wheat flour and citrate over 72 h. the 27-kda enzyme was a metalloprotease (sensitive to edta, egta and phenanthroli ...200515862448
pedobacter himalayensis sp. nov., from the hamta glacier located in the himalayan mountain ranges of india.strain hhs 22(t) was isolated from a glacial water sample from the snout of the hamta glacier located in the himalayan mountain ranges of india. phenotypic, chemotaxonomic and phylogenetic analyses established the affiliation of the isolate to the genus pedobacter. hhs 22(t) exhibits high 16s rrna gene sequence similarity with pedobacter cryoconitis (98 %). however, the level of dna-dna relatedness between hhs 22(t) and p. cryoconitis is only 42 %. furthermore, hhs 22(t) differs from p. cryoconi ...200515879237
pedobacter ginsengisoli sp. nov., a dnase-producing bacterium isolated from soil of a ginseng field in south korea.a gram-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated gsoil 104t, was isolated from a soil sample from a ginseng field in pocheon province (south korea) and was characterized taxonomically by using a polyphasic approach. on the basis of 16s rrna gene sequence similarities, strain gsoil 104t was shown to belong to the family sphingobacteriaceae, being related to pedobacter africanus dsm 12126t (97.0%), pedobacter caeni lmg 22862t (96.9%), pedoba ...200617082391
effect of temperature on growth parameters of psychrophilic bacteria and yeasts.three bacterial (pedobacter heparinus, pedobacter piscium, pedobacter cryoconitis) and three yeast strains (saccharomyces cerevisiae, leucosporidiella creatinivora, rhodotorula glacialis) of different thermal classes (mesophiles and psychrophiles) were tested for the effect of temperature on a range of growth parameters, including optical density, viable cell numbers, and cell dry mass, in order to determine the temperature conditions under which maximum biomass formation is obtained. maximum va ...200919057843
characterisation of a cryptic plasmid from an antarctic bacterium pedobacter cryoconitis strain bg5.a cryptic plasmid, pmwhk1 recovered from an antarctic bacterium pedobacter cryoconitis bg5 was sequenced and characterised. the plasmid is a circular 6206bp molecule with eight putative open reading frames designated as orf1, orf2, orf3, orf4, orf5, orf6, orf7 and orf8. all the putative open reading frames of pmwhk1 are found to be actively transcribed. proteins encoded by orf2 and orf4 are predicted to be responsible for the mobilization and replication of the plasmid respectively. orf4 shares ...201223266397
treatment of amphibians infected with chytrid fungus: learning from failed trials with itraconazole, antimicrobial peptides, bacteria, and heat therapy.amphibian conservation goals depend on effective disease-treatment protocols. desirable protocols are species, life stage, and context specific, but currently few treatment options exist for amphibians infected with the chytrid fungus batrachochytrium dendrobatidis (bd). treatment options, at present, include antifungal drugs and heat therapy, but risks of toxicity and side-effects make these options untenable in some cases. here, we report on the comparison of several novel treatments with a mo ...201222422126
proteome characterization of the unsequenced psychrophile pedobacter cryoconitis using 15n metabolic labeling, tandem mass spectrometry, and a new bioinformatic workflow.organisms without a sequenced genome and lacking a complete protein database encounter an added level of complexity to protein identification and quantitation. de novo sequencing, new bioinformatics tools, and mass spectrometry (ms) techniques allow for advances in this area. here, the proteomic characterization of an unsequenced psychrophilic bacterium, pedobacter cryoconitis, is presented employing a novel workflow based on (15) n metabolic labelling, 2de, ms/ms, and bioinformatics tools. two ...201222539428
complete genome sequence of pedobacter cryoconitis pamc 27485, a crispr-cas system-containing psychrophile isolated from antarctica.pedobacter cryoconitis pamc 27485, an aerobic, gram-negative, facultatively psychrophilic bacterium, was isolated from antarctic soil. here we report the complete genome of p. cryoconitis pamc 27485, which contains a type ii crispr-cas system and genes encoding useful enzymes (e.g. proteases). the genome sequence of p. cryoconitis pamc 27485 could provide insights into its adaptive immune system against foreign genetic elements and biotechnological potential.201627015980
development of a semidefined growth medium for pedobacter cryoconitis bg5 using statistical experimental design.pedobacter cryoconitis bg5 are psychrophiles isolated from the cold environment and capable of proliferating and growing well at low temperature regime. their cellular products have found a broad spectrum of applications, including in food, medicine, and bioremediation. therefore, it is imperative to develop a high-cell density cultivation strategy coupled with optimized growth medium for p. cryoconitis bg5. to date, there has been no published report on the design and optimization of growth med ...201626759918
pedobacter lusitanus sp. nov., a new bacterial species isolated from sludge of a deactivated uranium mine in portugal.strain nl19t is a gram negative aerobic bacterium that was isolated from sludge of a deactivated uranium mine in portugal. 16s rrna gene sequence analysis revealed that strain nl19t is a member of the genus pedobacter and closely related to the strains pedobacter himalayensis mtcc 6384t, pedobacter cryoconitis dsm 14825t, pedobacter westerhofensis dsm 19036t and p. hartonius dsm 19033t. it has a dna g+c content of 40.8 mol%, which agreed with the genus description. the main fatty acids include c ...201728109203
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