Publications

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description of enteromyxum scophthalmi gen. nov., sp. nov. (myxozoa), an intestinal parasite of turbot (scophthalmus maximus l.) using morphological and ribosomal rna sequence data.a new myxozoa species causing enteritis and death in cultured turbot, scophthalmus maximus, is described at light and electron microscope levels. in addition, small subunit ribosomal rna gene sequences (ssu rdna) from the new species and from similar myxozoans were obtained and used for phylogenetic inference, as complementary criteria to resolve its taxonomic classification. the new parasite is closely related to myxidium leei, another enteric histozoic species from marine fish. however, the as ...200212003061
gilthead seabream ( sparus aurata l.) innate defence against the parasite enteromyxum leei (myxozoa).the humoral innate immune response of gilthead seabream (sparus aurata l.) against the myxozoan enteromyxum leei has been studied. at 10, 22, 38, 52 and 108 days of cohabitation fish were sampled to examine gut histology and to determine serum innate immune parameters and the mrna expression of pro-inflammatory cytokines (il-1beta and tnfalpha) in head-kidney. the parasite was successfully transmitted to 45% of the recipient fish and prevalence reached a maximum (62.5%) at the last sampling time ...200616393358
temperature effects on the development of enteromyxum spp. (myxozoa) in experimentally infected tiger puffer, takifugu rubripes (temminck & schlegel).the effects of water temperature on the development of the enteric myxosporeans, enteromyxum fugu and enteromyxum leei, were investigated in experimentally infected tiger puffer, takifugu rubripes. after naïve tiger puffer were fed gut tissue infected with both e. fugu and e. leei, they were divided into separate tanks and kept at different constant temperature regimes between 10 and 25 degrees c. regardless of the water temperature tested, e. fugu was consistently detected with a high prevalenc ...200616948706
cell-mediated cytotoxicity is the main innate immune mechanism involved in the cellular defence of gilthead seabream (teleostei: sparidae) against enteromyxum leei (myxozoa).the cellular innate immune response of gilthead seabream (sparus aurata l.) against the myxozoan enteromyxum leei was studied. enteromyxosis was transmitted by maintaining uninfected fish (recipients) together with infected animals. a group of fish not exposed to the infection served as controls. after 10, 22, 38, 52 and 108 days, control and recipient fish were sampled and leucocyte subpopulations and cellular immune responses (leucocyte peroxidases, phagocytosis, respiratory burst and cytotoxi ...200617096645
experimental transmission of enteromyxum leei to freshwater fish.the myxosporean enteromyxum leei is known to infect a wide range of marine fish hosts. the objective of the present study was to determine whether freshwater fish species are also receptive hosts to this parasite. seventeen species of freshwater fish were experimentally fed e. leei-infected gut tissue from donor gilthead sea bream sparus aurata obtained from a commercial sea bream cage farm. four of the tested species, tiger barb puntius tetrazona, zebra danio danio rerio, oscar astronotus ocell ...200617140140
an unidentified epi-epithelial myxosporean in the intestine of gilthead sea bream sparus aurata l.in the course of experimental infections of gilthead sea bream sparus aurata with the myxozoan enteromyxum leei, stages of an unidentified myxozoan were observed attached to the intestinal brush border of some fish. infection levels of the parasite, which was named "epi-epithelial myxosporean" (eem) were recorded, and its structure was studied by light microscopy (lm) and electron microscopy (em). in situ hybridisation (ish) probes specific for e. leei were developed and used to differentiate be ...200717333277
effect of host factors and experimental conditions on the horizontal transmission of enteromyxum leei (myxozoa) to gilthead sea bream, sparus aurata l., and european sea bass, dicentrarchus labrax (l.). 200717394527
sharpsnout sea bream (diplodus puntazzo) humoral immune response against the parasite enteromyxum leei (myxozoa).the humoral innate immune response of sharpsnout seabream diplodus puntazzo against the myxozoan enteromyxum leei was studied. enteromyxosis was transmitted by cohabitation and a group of uninfected fish served as control. at 5, 12, 19, 26, 40 and 55 days post-exposure (p.e.), control and recipient fish were sampled to determine the prevalence of infection and some humoral innate immune parameters (antiprotease, antitumoral and peroxidase activities). prevalence of infection was high from day 12 ...200717475509
chronic exposure to the parasite enteromyxum leei (myxozoa: myxosporea) modulates the immune response and the expression of growth, redox and immune relevant genes in gilthead sea bream, sparus aurata l.the myxosporean parasite enteromyxum leei invades the intestine of gilthead sea bream producing a slow-progressing disease, which may end in the death of fish. the present work aimed to better know the host immune response and the underlying molecular mechanisms, which may help to understand why some individuals seem to be refractory to the disease. three main aspects involved in fish health and welfare (immune, growth and redox status) were studied in fish exposed to e. leei-contaminated efflue ...200818343157
histopathology and cellular response in enteromyxum leei (myxozoa) infections of diplodus puntazzo (teleostei).enteromyxum leei is an intestinal parasite responsible for serious outbreaks in mediterranean sharpsnout sea bream diplodus puntazzo. e. leei infection was experimentally transmitted to healthy d. puntazzo (r) by cohabitation with infected donor fish. haematological changes and histopathological damage were evaluated in relation to the course of infection. the prevalence of infection in r fish was 100% from day 10 post-exposure (p.e.) onwards, and the infection intensity and histopathological da ...200818373973
ultrastructure of enteromyxum leei (diamant, lom, & dyková, 1994) (myxozoa), an enteric parasite infecting gilthead sea bream (sparus aurata) and sharpsnout sea bream (diplodus puntazzo).the ultrastructure of the developmental stages of the myxozoan enteromyxum leei parasitizing gilthead seabream (sparus aurata) intestine and sharpsnout sea bream (diplodus puntazzo) intestine and gallbladder are described. the earliest stage observed was a small dense trophozoite located among enterocytes. proliferative stages, observed intercellularly in the epithelium of the intestine and gallbladder as well as in the lumen, possessed the typical cell-in-cell configuration throughout their dev ...200818460155
histopathological changes caused by enteromyxum leei infection in farmed sea bream sparus aurata.histological examinations were carried out on the stomach, pyloric caeca and 4 different parts of the intestine, as well as the rectum, hepatopancreas, gall bladder and spleen of 52 sea bream sparus aurata spontaneously infected by enteromyxum leei. fifteen fish from a non-infected farm were included as a control. clinical signs appeared only in extensively and severely infected fish. we observed enteromyxum leei almost exclusively in the intestinal tract, and very rarely in the intrahepatic bil ...200818589998
lectinhistochemical detection of terminal carbohydrate residues in the enteric myxozoan enteromyxum leei parasitizing gilthead seabream sparus aurata (pisces: teleostei): a study using light and transmission electron microscopy.the presence of terminal carbohydrate residues in enteromyxum leei (diamant, lom et dyková, 1994) palenzuela, redondo et alvarez-pellitero, 2002 stages in gilthead seabream intestines was studied at light microscopy (lm) and transmission electron microscopy (tem) level using lectin histochemical techniques. abundant mannose and/or glucose residues were demonstrated by the intense staining caused by binding of biotinylated concanavalin a (con a), at both lm and tem. a clear positivity was also ob ...200920128238
carbohydrate patterns in the digestive tract of sparus aurata l. and psetta maxima (l.) (teleostei) parasitized by enteromyxum leei and e. scophthalmi (myxozoa).the influence of enteromyxum spp. infections on the carbohydrate patterns of the digestive tract of gilthead sea bream (gsb) sparus aurata l. and turbot (tb) psetta maxima (l.) has been studied. histochemical stainings to differentiate the types of mucins and lectin-binding assays to detect terminal carbohydrate residues were applied to histological sections of gsb and tb uninfected or infected by enteromyxum leei and e. scophthalmi, respectively. the number of intestinal gc decreased in severel ...201020601108
the nutritional background of the host alters the disease course in a fish-myxosporean system.the aim of the present work was to determine if a practical plant protein-based diet containing vegetable oils (vo) as the major lipid source could alter the disease course when challenged with the myxosporean enteromyxum leei, a wide-spread parasite in the mediterranean basin causing heavy economic losses. gilthead sea bream (sparus aurata) fed for 9 months either a fish oil (fo) diet or a blend of vos at 66% of replacement (66vo diet) were challenged by exposure to parasite-contaminated water ...201020947256
novel horizontal transmission route for enteromyxum leei (myxozoa) by anal intubation of gilthead sea bream sparus aurata.the aim of the present study was to determine whether enteromyxum leei, one of the most threatening parasitic diseases in mediterranean fish culture, could be transmitted by peranal intubation in gilthead sea bream sparus aurata l. fish were inoculated either orally or anally with intestinal scrapings of infected fish in 3 trials. oral transmission failed, but the parasite was efficiently and quickly transmitted peranally. prevalence of infection was 100% at 60 d post inoculation (p.i.) in trial ...201021166314
molecular characterization and expression analysis of six peroxiredoxin paralogous genes in gilthead sea bream (sparus aurata): insights from fish exposed to dietary, pathogen and confinement stressors.the aim of this work was to underline the physiological role of the antioxidant peroxiredoxin (prdx) family in gilthead sea bream (sparus aurata l.), a perciform fish extensively cultured in the mediterranean area. first, extensive blast searches were done on the gilthead sea bream cdna database of the aquamax european project (www.sigenae.org/iats), and six contigs were unequivocally identified as prdx1-6 after sequence completion by rt-pcr. the phylogenetic analysis evidenced three major clade ...201121640832
molecular profiling of the gilthead sea bream (sparus aurata l.) response to chronic exposure to the myxosporean parasite enteromyxum leei.the aim of the present work was to investigate the transcriptome response of gilthead sea bream (sparus aurata) after challenge with the myxosporean enteromyxum leei, a wide-spread enteric parasite causing heavy economic losses in mediterranean sparid farms. this parasite causes severe desquamative enteritis which usually leads to death of the fish, and there are no preventative or curative treatments for this enteromyxosis. after 113 days of exposure to parasite-contaminated effluent, fish were ...201121803425
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