Publications

southern hemisphere mollusc diseases and an overview of associated risk assessment problems.	in australia and new zealand, bonamia sp. causes epizootics in flat oysters (tiostrea chilensis, ostrea angasi); marteilia sydneyi and mikrocytos roughleyi cause mortality in farmed rock oysters (saccostrea commercialis); and perkinsus olseni is pathogenic in abalone (haliotis spp.). marteilia lengehi, marteilioides branchialis, other marteilioides spp. and two species of haplosporidium are regarded as potential pathogens. a review of the pathogens causing diseases listed in the office internati ...	1996	8890381
detection of bonamia ostreae based on small subunit ribosomal probe.	bonamia ostreae is a protozoan parasite of the flat oyster, ostrea edulis, which has caused significant loss of oysters in europe over the last decade. b. ostreae was purified from infected flat oysters and dna was extracted. the nearly complete small subunit rdna gene of b. ostreae was amplified using universal oligonucleotides and the pcr product was cloned and sequenced. blast research with this sequence revealed similarities to haplosporidium nelsoni, haplosporidium costale, and minchinia te ...	2000	10963400
bonamia exitiosus n. sp. (haplosporidia) infecting flat oysters ostrea chilensis in new zealand.	bonamia sp. is a pathogenic parasite that occurs in the haemocytes of dredge oysters ostrea chilensis philippi in new zealand. ultrastructurally it resembles other haplosporidians in the possession of haplosporosomes, haplosporogenesis, persistence of mitotic microtubules during interphase and of the nuclear envelope during mitosis, and occurrence of a diplokaryotic or multi-nucleate plasmodial stage. another stage containing a large vacuole derived from enlargement of 1 or more mitochondria has ...	2001	11797917
occurrence of the haemocyte parasite bonamia sp. in flat oysters ostrea puelchana farmed in san antonio bay (argentina).	culture of native flat oysters ostrea puelchana d'orbigny in san antonio bay (san matías gulf, argentina) began in 1995. after elevated mortality (33%) occurred in september 1996, 18 mo after immersion, histopathological analysis and evaluation of parasitic prevalence was carried out. in october 1997, after 31 mo of cultivation, cumulative mortality was 80%, and in december of the same year, when individuals reached marketable size, mortality was 95% and culture was discontinued. the present stu ...	2005	15819438
bonamia perspora n. sp. (haplosporidia), a parasite of the oyster ostreola equestris, is the first bonamia species known to produce spores.	examination of the oyster ostreola equestris as a potential reservoir host for a species of bonamia discovered in crassostrea ariakensis in north carolina (nc), usa, revealed a second novel bonamia sp. histopathology, electron microscopy, and molecular phylogenetic analysis support the designation of a new parasite species, bonamia perspora n. sp., which is the first bonamia species shown to produce a typical haplosporidian spore with an orifice and hinged operculum. spores were confirmed to be ...	2006	16872291
strong seasonality of bonamia sp. infection and induced crassostrea ariakensis mortality in bogue and masonboro sounds, north carolina, usa.	asian oyster crassostrea ariakensis is being considered for introduction to atlantic coastal waters of the usa. successful aquaculture of this species will depend partly on mitigating impacts by bonamia sp., a parasite that has caused high c. ariakensis mortality south of virginia. to better understand the biology of this parasite and identify strategies for management, we evaluated its seasonal pattern of infection in c. ariakensis at two north carolina, usa, locations in 2005. small (<50 mm) t ...	2008	18455182
interacting effects of temperature and salinity on bonamia sp. parasitism in the asian oyster crassostrea ariakensis.	the proposition to introduce the asian oyster crassostrea ariakensis to the mid-atlantic region of the usa is being considered with caution, particularly after the discovery of a novel microcell haplosporidian parasite, bonamia sp., in north carolina. although this parasite was found to be pathogenic in c. ariakensis under warm euhaline conditions, its persistence in c. ariakensis exposed to various temperature and salinity combinations remained unresolved. in this laboratory experiment, we test ...	2008	18456278
first report of a mikrocytos-like parasite in european oysters ostrea edulis from canada after transport and quarantine in france.	as part of a disease resistance experiment, 112 apparently healthy european flat oysters ostrea edulis l. were exported from canada (nova scotia) into france to test their susceptibility to bonamia ostreae infection. twelve oysters died in transit and 17 others died within 2 wk of laboratory quarantine acclimation. all oysters were examined histologically, and the 17 that died during quarantine were assayed for microcells (bonamia sp. and mikrocytos mackini) using molecular techniques. a microce ...	2008	18714681
ultrastructure of bonamia sp. in ostrea chilensis in chile.	oyster ostrea chilensis samples were collected from quihua island, chile, in december 2003 and february 2005, and examined in may 2004, and march, april and july 2005, for an ultrastructural comparison of the chilean bonamia sp. with other bonamia spp. only uni-nucleate stages were encountered, except in the july sample. the chilean parasite differs from b. perspora in the apparent lack of a plasmodial stage and of sporulation. it resembles b. ostreae in size, the low number of mitochondrial pro ...	2009	19750808
observation of a bonamia sp. infecting the oyster ostrea stentina in tunisia, and a consideration of its phylogenetic affinities.	the small non-commercial oyster ostrea stentina co-occurs with commercially important ostrea edulis in the mediterranean sea, yet its disposition with respect to the destructive pathogens bonamia ostreae and marteilia refringens is unknown. we began an evaluation of the bonamia spp. infection status of o. stentina from hammamet, tunisia, in june 2007 using polymerase chain reaction diagnostics followed by histology and in situ hybridization. of 85 o. stentina sampled, nine were pcr-positive for ...	2010	20036670
detection of bonamia ostreae and b. exitiosa (haplosporidia) in ostrea edulis from the adriatic sea (italy).	the flat oyster ostrea edulis l. is widespread along the italian coasts. in particular, the manfredonia gulf (adriatic sea) represents an important site where natural beds subsist. previous monitoring conducted in 1990 by light microscopy and ultrastructural studies revealed the presence of bonamia-like microcell parasites in some flat oysters: following this observation, a new sampling of o. edulis was carried out at this location in 2007. of 750 oysters collected, 3 showed the presence of unin ...	2010	20391915
gross signs and histopathology of ostrea puelchana infected by a bonamia exitiosa-like parasite (haplosporidia).	haplosporidian microcells belonging to the genus bonamia parasitise various species of oysters around the world. in argentina, bonamia sp. was the causative agent of mass mortality among ostrea puelchana cultured in san antonio bay (san matías gulf), and it was detected in natural beds inside san matías gulf. in order to describe the gross and histopathological signs caused by bonamia sp. in o. puelchana, cultured and wild oysters were sampled and analysed by traditional techniques including hea ...	2010	20481090
diseases of oysters crassostrea ariakensis and c. virginica reared in ambient waters from the choptank river, maryland and the indian river lagoon, florida.	to assess potential benefits and liabilities from a proposed introduction of asian suminoe oysters, susceptibilities of exotic crassostrea ariakensis and native c. virginica oysters were compared during exposures to pathogens endemic in temperate, mesohaline waters of chesapeake bay and sub-tropical, polyhaline atlantic waters of southern florida, usa. cohorts of diploid, sibling oysters of both species were periodically tested for diseases while reared in mesocosms receiving ambient waters from ...	2012	23324414