Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| distribution and seasonal variations in levels of three native gnrhs in the brain and pituitary of perciform fish. | specific and sensitive radioimmunoassays (rias) were newly developed for two types of gonadotropin-releasing hormone (gnrh), namely, seabream (sb) gnrh and chicken (c) gnrh-ii. we employed these two rias together with a previously reported ria for salmon (s) gnrh to study the presence and regional distribution of these three gnrhs in the brains and pituitaries of four perciform fishes (red seabream, pagrus major; black seabream, acanthopagrus schlegeli; striped knifejaw, oplegnathus fasciatus; a ... | 1999 | 10201813 |
| biochemical responses of fish exposed to a harmful dinoflagellate cochlodinium polykrikoides. | to elucidate the ichthyotoxic mechanisms of a harmful dinoflagellate cochlodinium polykrikoides, biochemical responses of fish exposed to blooms were investigated. particularly, based on our finding that oxidative damages of gill were associated with fish mortality (j. plankton res. 21 (1999) 2105-2115), dysfunction of ion-transporting enzymes and secretion of gill mucus of fish exposed to this bloom species were examined. the susceptibilities of several fishes to c. polykrikoides were different ... | 2000 | 11077057 |
| complete genomic dna sequence of rock bream iridovirus. | iridovirus is a causative agent of epizootics among cultured rock bream (oplegnathus fasciatus) in korea. here, we report the complete genomic sequence of rock bream iridovirus (rbiv). the genome of rbiv was 112080 bp long and contained at least 118 putative open reading frames (orfs), and its genome organization was similar to that of infectious spleen and kidney necrosis virus (isknv). of the rbiv's 118 orfs, 85 orfs showed 60-99% amino acid identity to those of isknv. phylogenetic analysis of ... | 2004 | 15246274 |
| susceptibility of marine fish species to a megalocytivirus, turbot iridovirus, isolated from turbot, psetta maximus (l.). | turbot iridovirus (tbiv), a member of the genus megalocytivirus in the family iridoviridae, was isolated from diseased turbot, psetta maximus (l.), in korea in 2003. in this study, experimental infection of turbot, japanese flounder, paralichthys olivaceus (temminck & schlegel), and rock bream, oplegnathus fasciatus (temminck & schlegel), with tbiv was performed to evaluate the viral susceptibility of these fish species. after virus exposure, the mortalities of turbot reared at 22 and 25 degrees ... | 2006 | 16866925 |
| expression and serological application of a capsid protein of an iridovirus isolated from rock bream, oplegnathus fasciatus (temminck & schlegel). | iridoviruses infect a wide variety of wild and cultured fish. those iridoviruses belonging to the genus ranavirus, in the iridoviridae family, cause systemic disease in infected animals with a high morbidity and mortality. this paper reports the cloning, sequencing, and expression of the rock bream iridovirus (rbiv) major capsid protein (mcp) in an escherichia coli expression system for subsequent immunological studies. the completeness of the expressed protein was confirmed by peptide mass fing ... | 2007 | 17958613 |
| phylogenetic analysis of betanodaviruses isolated from cultured fish in korea. | since the publication of the first report on fish nodaviruses in korea in 1998, fish nodaviruses have caused widespread epizootic events among various fish species in korea. however, the genotypes of fish nodaviruses in korea have not yet been determined due to a lack of information about their nucleotide sequences. in this study, we isolated 5 fish nodaviruses from 4 fish species cultured in 4 different regions in korea: rock bream oplegnathus fasciatus, japanese flounder paralichthys olivaceus ... | 2007 | 18062469 |
| comparison of surface plasmon resonance imaging and enzyme-linked immunosorbent assay for the detection of antibodies against iridovirus in rock bream (oplegnathus fasciatus). | a protein chip based on surface plasmon resonance imaging (spri) was developed for detecting fish iridovirus antibody using a recombinant 50-kda fragment of major capsid protein (mcp) as an antigen. the diagnostic potential of spri for measuring antibodies to the iridovirus mcp was compared with that of a conventional enzyme-linked immunosorbent assay (elisa) using 40 juvenile rock bream (oplegnathus fasciatus) serum samples in a nursery. there was a strong positive correlation between the spri ... | 2007 | 17609354 |
| complete mitochondrial genome of the rock bream oplegnathus fasciatus (perciformes, oplegnathidae) with phylogenetic considerations. | we determined the complete nucleotide sequence of the mitochondrial genome for the rock bream, oplegnathus fasciatus (perciformes, oplegnathidae). this mitochondrial genome, consisting of 16,511 base pairs (bp), encoded genes for 13 protein-coding genes, two ribosomal rnas, 22 transfer rnas, and a noncoding control region like those found in other vertebrates, with the gene order identical to that of typical vertebrates. most of the genes of o. fasciatus were encoded on the h-strand, while the n ... | 2007 | 17258872 |
| transmission of iridovirus from freshwater ornamental fish (pearl gourami) to marine fish (rock bream). | freshwater pearl gourami trichogaster leeri and seawater rock bream oplegnathus fasciatus infected by the iridoviruses pgiv-sp and ivs-1 were carrying similar numbers of viral particles (2.52 x 10(8) and 2.46 x 10(8) viral genome copies mg(-1) spleen tissue, respectively). the viral genome copy number for both iridoviruses decreased much faster in seawater than in freshwater, reaching a concentration of less than 0.5%, versus 26 to 54% in freshwater, after 4 d of incubation at 25 degrees c. the ... | 2008 | 19062750 |
| vaccination of rock bream, oplegnathus fasciatus (temminck & schlegel), using a recombinant major capsid protein of fish iridovirus. | 2008 | 18577102 | |
| production of polyclonal antibody against recombinant orf 112 l of rock bream (oplegnathus fasciatus) iridovirus (rbiv) and in vitro neutralization. | rock bream iridovirus (rbiv) is a causative agent of epizootics among cultured rock bream (oplegnathus fasciatus) in korea. in this study, the immunogenic property of ankyrin repeats gene (orf 112l) from rbiv was evaluated to develop vaccines against rbiv. orf 112l of rbiv was cloned into expression vector of pgex-4t-1. the recombinant protein was successfully expressed using e. coli bl21 (de3). the soluble recombinant rbiv protein was applied to affinity column for the purification of the prote ... | 2008 | 19195399 |
| differential modulations of two glyceraldehyde 3-phosphate dehydrogenase mrnas in response to bacterial and viral challenges in a marine teleost oplegnathus fasciatus (perciformes). | glyceraldehyde 3-phosphate dehydrogenase (gapdh) has been recognized as an invariant internal control for various gene expression studies due to its classical housekeeping role in the glycolytic metabolism. however recently, this enzyme has been proven to be a multifunctional protein involved in diverse non-glycolytic activities. in the present study, two paralogue isoforms of gapdh mrnas were characterized from a marine teleost species, rockbream (oplegnathus fasciatus; perciformes) and their t ... | 2008 | 18692141 |
| functional characterization of the rnase iii gene of rock bream iridovirus. | all of the fully sequenced iridoviruses have an orf resembling a putative rnase iii gene. however, to the best of our knowledge, functional characterization of the iridovirus-encoded rnase iii has not been done. in the present study, we have characterized the putative rnase iii of rock bream iridovirus (rbiv), the major cause of mass mortality of cultured rock bream oplegnathus fasciatus in korea. rbiv rnase iii has a single n-terminal endonuclease domain followed by a c-terminal double-stranded ... | 2008 | 18641914 |
| influence of temperature shifts on the onset and development of red sea bream iridoviral disease in rock bream oplegnathus fasciatus. | the effects of various water temperature treatments on the development of red sea bream iridovirus disease (rsivd) in rock bream oplegnathus fasciatus challenged with iridovirus sachun (ivs-1) were determined by measuring the mortality and the viral concentration in the spleen of infected fish. experimental infections of rock bream with ivs-1 at water temperatures of 18, 21, and 25 degrees c resulted in a cumulative mortality of 100%, but infections at 13 degrees c resulted in 0% mortality, even ... | 2009 | 19565697 |
| characterization of virus distribution in rock bream (oplegnathus fasciatus; temminck and schlegel) infected with megalocytivirus. | the distribution of virus-infected cells in the organs of rock bream naturally infected with megalocytivirus is reported. examination of sections of liver, spleen and kidney stained by haematoxylin and eosin (he) and periodic acid schiff (pas) revealed the presence of swollen and degenerate cells having morphology consistent with leucocytes. many of these cells were shown to contain viral dna by in-situ hybridization (ish). cells containing viral dna were also found in the connective tissue of o ... | 2009 | 19477463 |
| molecular cloning of rock bream (oplegnathus fasciatus) tumor necrosis factor-alpha and its effect on the respiratory burst activity of phagocytes. | rock bream (oplegnathus fasciatus) tumor necrosis factor-alpha (rbtnf-alpha) gene was cloned, recombinantly produced, and the effect of the recombinant rbtnf-alpha on the respiratory burst activity of rock bream phagocytes was analyzed. structurally, genomic dna of rbtnf-alpha was comprised with four exons and three introns, and deduced amino acid sequence of its cdna possessed the tnf family signature, a transmembrane domain, a protease cleavage site, and two cysteine residues, which are the ty ... | 2009 | 19628044 |
| molecular cloning and expression analysis of three mx isoforms of rock bream, oplegnathus fasciatus. | complementary dnas (cdnas) corresponding to three isoforms of rock bream (oplegnathus fasciatus) mx (rbmx1, rbmx2 and rbmx3) were cloned using race reactions. analysis of deduced amino acid sequences revealed that the tripartite gtp-binding domain, the dynamine family signature and the leucine zipper repeat were present in all three rock bream mx isoforms. cloning of genomic dna sequence and southern blot analysis showed that three rock bream mx isoforms were encoded by different genomic loci, a ... | 2009 | 18948206 |
| withdrawn: a mannose binding lily type lectin-3 (ofltl-3) from rock bream (oplegnathus fasciatus): characterization, cloning and gene expression. | this article has been withdrawn at the request of the editor. the publisher apologizes for any inconvenience this may cause. the full elsevier policy on article withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. | 2010 | 21044890 |
| retracted: molecular characterization and expression analysis of lily type lectin-1 (ofltl-1) in rock bream (oplegnathus fasciatus). | this article has been withdrawn at the request of the author(s) and/or editor. the publisher apologizes for any inconvenience this may cause. the full elsevier policy on article withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. | 2010 | 21193051 |
| molecular cloning and expression analysis of double-stranded rna-dependent protein kinase (pkr) in rock bream (oplegnathus fasciatus). | we have cloned and characterized rock bream double-stranded rna-dependent protein kinase (pkr), which is a key component of type i ifn inducible innate immune system. full-length rock bream pkr cdna consists of 2115bp orf encoding 704 amino acids, 124bp 5' utr, and 529bp 3' utr. conserved domain analysis revealed that rock bream pkr contained two tandem dsrbm and kinase domain consisted of 11 kinase sub-motifs, which are characteristics found in other pkrs. rock bream pkr was constitutively expr ... | 2010 | 19729206 |
| molecular characterization and expression analysis of cathepsin b and l cysteine proteases from rock bream (oplegnathus fasciatus). | cathepsins are lysosomal cysteine proteases of the papain family that play an important role in intracellular protein degradation and turn over within the lysosomal system. in the present study, full-length sequences of cathepsin b (rbcathepsin b) and l (rbcathepsin l) were identified after transcriptome sequencing of rock bream oplegnathus fasciatus mixed tissue cdna. cathepsin b was composed of 330 amino acid residues with 36 kda predicted molecular mass. rbcathepsin l contained 336 amino acid ... | 2010 | 21195770 |
| characterization and expression analysis of a goose-type lysozyme from the rock bream oplegnathus fasciatus, and antimicrobial activity of its recombinant protein. | lysozyme (muramidase) represents an important defense molecule of the fish innate immune system. known for its bactericidal properties, lysozyme catalyzes the hydrolysis of β-(1,4)-glycosidic bonds between the n-acetyl glucosamine and n-acetyl muramic acid in the peptidoglycan layer of bacterial cell walls. in this study, the complete coding sequence of a g-type lysozyme (rbglyz) was identified in the oplegnathus fasciatus rock bream fish genome by means of multi-tissue normalized cdna pyroseque ... | 2010 | 21167286 |
| characterization and expression analysis of the myeloid differentiation factor 88 (myd88) in rock bream oplegnathus fasciatus. | myeloid differentiation factor 88 (myd88) is a universal adaptor protein able to activate nuclear factor-kappa b (nf-κb) through interactions with interleukin-1 receptor (il-1r) and the toll-like receptors (tlrs), with the exception of tlr3. here, we describe the identification of myd88 from the rock bream fish oplegnathus fasciatus and its characterization based on gs-flx™ sequencing. the cdna of rock bream myd88 was found to be composed of 1626 bp, with an 867 bp open reading frame that encode ... | 2010 | 21152988 |
| small subunit ribosomal rna and mitochondrial cytochrome c oxidase subunit 1 gene sequences of 21 strains of the parasitic scuticociliate miamiensis avidus (ciliophora, scuticociliatia). | the scuticociliate miamiensis avidus is a histophagous parasite that causes high mortality in cultured marine fishes. small subunit ribosomal rna (ssu rrna) and mitochondrial cytochrome c oxidase subunit 1 (cox1) genes were analyzed for 21 strains of m. avidus isolated from diseased olive flounder (paralichthys olivaceus), ridged-eye flounder (pleuronichthys cornutus), and spotted knifejaw (oplegnathus fasciatus) in korea and japan (collected in 2003-2007). analysis of ssu rrna gene sequences (1 ... | 2010 | 21113725 |
| galectin-1 (ofglec-1) from rock bream (oplegnathus fasciatus): identification, characterization, gene expression and functional properties. | lectins may function as pattern-recognition receptors (prrs) and play important roles in immune responses. in this study, a novel galectin-1 (designated ofglec-1) gene was identified from rock bream, oplegnathus fasciatus, cdna library. the complete cdna sequence of the ofglec-1 gene is 993 base pairs in length, and encodes 135 amino acids. ofglec-1 shares a highly conserved carbohydrate recognition domain with galectin-1 from other fishes. the mrna expressions of ofglec-1 in healthy and bacteri ... | 2010 | 21073891 |
| heparin cofactor ii (rbhcii) from rock bream (oplegnathus fasciatus): molecular characterization, cloning and expression analysis. | heparin cofactor (hcii) is a serine protease inhibitor (spi), and plays important physiological roles in various biological events including hemostasis. the gene encoding the hcii was isolated from gs-flx™ genomic data of rock bream (oplegnathus fasciatus), designated as rbhcii. the rbhcii (1950 bp) consists of a 1512 bp open reading frame (orf) encoding 504 amino acids (aa), with a signal peptide of 19 aa residues. the predicted molecular mass and the estimated isoelectric point of rbhcii were ... | 2010 | 20951808 |
| permanent genetic resources added to molecular ecology resources database 1 october 2010-30 november 2010. | this article documents the addition of 277 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: ascochyta rabiei, cambarellus chapalanus, chionodraco hamatus, coptis omeiensis, cynoscion nebulosus, daphnia magna, gerbillus nigeriae, isurus oxyrinchus, lates calcarifer, metacarcinus magister, oplegnathus fasciatus, pachycondyla verenae, phaethon lepturus, pimelodus grosskopfii, rotylenchulus reniformis, scomberomorus niphonius, sep ... | 2011 | 21429157 |
| molecular characterisation and expression analysis of a fish-egg lectin in rock bream, and its response to bacterial or viral infection. | a fish-egg lectin, rbfel, was identified from rock bream (oplegnathus fasciatus) and its expression analysed. in both vertebrates and invertebrates, carbohydrate-binding proteins (lectins) play an important role in innate immunity against microbial invasion. here, we report the cloning of a fish-egg lectin from rock bream using a combination of expression sequence tag (est) analyses. the full-length cdna of rbfel is composed of 1512-bp with a 780-bp orf that encodes 259 amino acid residues. the ... | 2011 | 22056500 |
| molecular cloning and expression analysis of two distinct f-type lectins from the rock bream, oplegnathus fasciatus. | several lectin families characterized by distinct signature sequence motifs and structural folds, such as c-type, peptidoglycan recognition protein, ficolin, pentraxins, and most recently galectins, have been implicated in immune surveillance. in this study, two distinct f-type lectins rbftl-1 and rbftl-2, from the rock bream (oplegnathus fasciatus), were identified and their expression was analyzed. the full-length cdna of rbftl-1 was composed of 1204bp with a 945-bp open reading frame (orf) th ... | 2011 | 21683734 |
| molecular identification and expression analysis of cathepsins o and s from rock bream, oplegnathus fasciatus. | cathepsins are lysosomal cysteine proteases belonging to the papain family, members of which play important roles in normal metabolism for maintenance of cellular homeostasis. rock bream (oplegnathus fasciatus) cathepsin o and s (rbctso and rbctss, respectively) cdnas were identified by expressed sequence tag (est) analysis of a lipopolysaccharide (lps)-stimulated rock bream liver cdna library. the full-length rbctso cdna (1698 bp) contained an open reading frame (orf) of 1017 bp encoding 338 am ... | 2011 | 21767649 |
| [characterization of an iridovirus detected in rock bream (oplegnathus fasciatus; temminck and schlegel)]. | during the summer of 2009, mass mortality was observed in cage-cultured rock bream (oplegnathus fasciatus; temminck and schlegel) in the liaoning province. histopathogic studies of the affected fish showed enlarged basophilic cells in the kidney and spleen. these necrotic cells were stained purple using haematoxylin and eosin (he). gf cell cultures showed advanced cytopathic effects after infection with virus supernatants from diseased fish homogenate. transmission electron microscopy revealed h ... | 2011 | 21528541 |
| rock bream (oplegnathus fasciatus) serpin, protease nexin-1: transcriptional analysis and characterization of its antiprotease and anticoagulant activities. | protease nexin-1 (pn-1) is a serine protease inhibitor (serpin) protein with functional roles in growth, development, patho-physiology and injury. here, we report our work to clone, analyze the expression profile and characterize the properties of the pn-1 gene in rock bream (rb), oplegnathus fasciatus. rbpn-1 encodes a peptide of 397 amino acids (aa) with a predicted molecular mass of 44 kda and a 23 aa signal peptide. rbpn-1 protein was found to harbor a characteristic serpin domain comprised ... | 2011 | 21419793 |
| molecular cloning and expression analysis of a thioredoxin from rock bream, oplegnathus fasciatus, and biological activity of the recombinant protein. | thioredoxins (trxs) are a family of small, highly conserved proteins that are essential for the maintenance of cellular homeostasis. trx1, which contains a conserved redox-active site, cys-gly-pro-cys, is a proinflammatory cytokine, b cell growth factor, macrophage migration inhibiting factor (mif), and an immune regulatory cytokine. the trx1 homologue cdna was isolated from the rock bream lps-stimulated liver cdna library, rbtrx1. rbtrx1 consists of 730 bp full-length cdna with a 324 bp open re ... | 2011 | 21402159 |
| sequence conservation in the internal transcribed spacers and 5.8s ribosomal rna of parasitic scuticociliates miamiensis avidus (ciliophora, scuticociliatia). | the scuticociliate miamiensis avidus is a histophagous parasite that causes high mortality in cultured marine fishes, with clinical signs of severe ulcers and hemorrhages in the skeletal muscle. the internal transcribed spacer (its) region, which is widely used in taxonomy and molecular phylogeny because of a high degree of variation, was compared for 21 cloned strains of m. avidus (ciliophora, scuticociliatia). these strains were isolated from olive flounder paralichthys olivaceus, ridged-eye f ... | 2011 | 21362496 |
| molecular cloning of rock bream's (oplegnathus fasciatus) tumor necrosis factor receptor-associated factor 2 and its role in nf-?b activiation. | tumor necrosis factor receptor-associated factor 2 (traf2) acts as a transducer of tumor necrosis factor-a (tnf-a)-triggered cell signals which results in inflammation, cell proliferation and antiapoptotic response. in this study, we have cloned cdna of rock bream (oplegnathus fasciatus) traf2, and analyzed its function in activation of nf-?b. the full length cdna of rock bream traf2 consisted of 95 bp 5' utr, 335 bp 3' utr, and 1563 bp orf encoding 520 amino acids that contained n-terminal ring ... | 2011 | 21320605 |
| protective effect of herbal and probiotics enriched diet on haematological and immunity status of oplegnathus fasciatus (temminck & schlegel) against edwardsiella tarda. | this study determines the effect of diet enriched with the herb baical skullcap scutellaria baicalensis, and/or probiotics lactobacillus sakei bk19 in rock bream, oplegnathus fasciatus (32 ± 3 g) against edwardsiella tarda. the changes in haematological parameters, innate immune response, and disease resistance were investigated after 1, 3, and 6 weeks. the white blood cell count (wbc: 10(4) mm(-3)), red blood cell count (rbc: 10(6) mm(-3)), and haemoglobin (hb: g dl(-1)) levels significant incr ... | 2011 | 21272648 |
| molecular identification and expression analysis of a natural killer cell enhancing factor (nkef) from rock bream oplegnathus fasciatus and the biological activity of its recombinant protein. | natural killer cell enhancing factor (nkef) belongs to the defined peroxiredoxin (prx) family. rock bream nkef cdna was identified by expressed sequence tag (est) analysis of rock bream liver that was stimulated with the lps. the full-length rbnkef cdna (1062 bp) contained an open reading frame (orf) of 594 bp encoding 198 amino acids. rbnkef was significantly expressed in the gill, liver, and intestine. mrna expression of nkef in the head kidney was examined under viral and bacterial challenge ... | 2011 | 24371552 |
| molecular cloning and characterisation of the rock bream, oplegnathus fasciatus, fas (cd95/apo-1), and its expression analysis in response to bacterial or viral infection. | fas belongs to the tumour necrosis factor (tnf) receptor superfamily and can transmit a death signal leading to apoptosis. in the present study, we isolated the full-length cdna for rock bream (oplegnathus fasciatus) fas (rbfas). the full-length rbfas cdna was 1770 bp long and contained an open reading frame of 957 bp that encoded 319 amino acid residues with a predicted molecular mass of 35.1 kda. the 319 amino-acid predicted rbfas sequence is homologous to other fas sequences, contains three c ... | 2011 | 24371547 |
| molecular characterization, immune responses and dna protection activity of rock bream (oplegnathus fasciatus), peroxiredoxin 6 (prx6). | in this study, we describe the molecular characterization, immune responses of rock bream, oplegnathus fasciatus peroxiredoxin 6 cdna (rbprx6) and dna protection activity of its recombinant protein. the full-length cdna sequence of rbprx6 was identified after pyrosequencing of rock bream cdna library. rbprx6 consists of 663 bp open reading frame (orf) that codes for a putative protein of 221 amino acids with predicted molecular mass of 27 kda. it showed characteristic peroxiredoxin super-family ... | 2012 | 22484606 |
| ontogeny of the digestive tract and enzymes in rock bream oplegnathus fasciatus (temminck et schlegel 1844) larvae. | histological development of the digestive tract and specific activities of three digestive enzymes (trypsin, alkaline phosphatase, and pepsin) were studied in rock bream oplegnathus fasciatus from hatching to 50 days after hatching (dah). at hatching, the digestive tract appeared as an undifferentiated straight tube and differentiated into the buccopharynx, esophagus, stomach, intestine, and rectum at mouth opening by 3 dah. the taste bud and mandibular teeth were present in the buccopharyx at 8 ... | 2012 | 21604160 |
| a novel acute phase reactant, serum amyloid a-like 1, from oplegnathus fasciatus: genomic and molecular characterization and transcriptional expression analysis. | acute phase response is a significant component of innate immunity, playing a vital role in the signaling processes and elimination of invading pathogens. acute phase proteins are synthesized in liver and secreted into the blood for transportation to an infection site, where the defense function is exerted. serum amyloid a (saa) and c-reactive proteins are the major positive acute phase proteins. in this study, we have identified and characterized a novel saa related gene from rock bream (oplegn ... | 2012 | 22504166 |
| cystatin b homolog from rock bream oplegnathus fasciatus: genomic characterization, transcriptional profiling and protease-inhibitory activity of recombinant protein. | cysteine proteases are present in all living organisms and, in animals, function in a vast array of physiological and pathological processes. cysteine protease inhibitors act upon the cysteine proteases to regulate their activity. the cystatin superfamily of cysteine protease inhibitors has members represented in all living organisms studied to date. here, we report the identification of a new member of the family 1 cystatin in oplegnathus fasciatus rock bream (denoted as rbcyt b) and the charac ... | 2012 | 22626887 |
| molecular cloning and functional characterization of two duplicated two-cysteine containing type i interferon genes in rock bream oplegnathus fasciatus. | two type i interferon (ifn) genes, designated as rbifn1 and rbifn2, have been cloned and characterized in rock bream. they are both comprised of 5 exons and 4 introns, and are closely linked on the rock bream chromosome in a unique head-to-head configuration. both genes encode 183 amino acid (aa) precursor with a putative 17 aa signal peptide in the n-terminal. only one amino acid divergence is present between two ifns. compared with the type i ifns in higher vertebrates, two rock bream ifns pos ... | 2012 | 22889848 |
| interferon regulatory factors 4 and 8 in rock bream, oplegnathus fasciatus: structural and expressional evidence for their antimicrobial role in teleosts. | the interferon regulatory factor (irf) members irf4 and irf8 contribute to b-lymphocyte development and can act as regulators of immunoglobulin (ig) light chain gene transcription. these two irfs are closely interrelated and are expressed at high levels in the lymphoid and myeloid cells of the immune system. in this study, the complete cdna and genomic sequences of rock bream irf4 (rbirf4) and irf8 (rbirf8) were identified by homology screening of a multi-tissue normalized cdna library and a bac ... | 2012 | 22885028 |
| genomic characterization and expression analysis of complement component 9 in rock bream (oplegnathus fasciatus). | the complement component 9 (c9) is a single-chain glycoprotein that mediates formation of the membrane attack complex (mac) on the surface of target cells. full-length c9 sequence was identified from a cdna library of rock bream (oplegnathus fasciatus), and its genomic sequence was obtained by screening and sequencing of a bacterial artificial chromosome (bac) genomic dna library of rock bream. the rock bream complement component 9 (rb-c9) gene contains 11 exons and 10 introns and is composed of ... | 2012 | 22796422 |
| caspase 3 from rock bream (oplegnathus fasciatus): genomic characterization and transcriptional profiling upon bacterial and viral inductions. | caspase 3 is a prominent mediator of apoptosis and participates in the cell death signaling cascade. in this study, caspase 3 was identified (rbcasp3) and characterized from rock bream (oplegnathus fasciatus). the full-length cdna of rbcasp3 is 2683 bp and contains an open reading frame of 849 bp, which encodes a 283 amino acid protein with a calculated molecular mass of 31.2 kda and isoelectric point of 6.31. the amino acid sequence resembles the conventional caspase 3 domain architecture, incl ... | 2012 | 22554851 |
| molecular identification and expression analysis of two distinct bpi/lbps (bactericidal permeability-increasing protein/lps-binding protein) from rock bream, oplegnathus fasciatus. | we identified two cdnas designated as rbbpi/lbp-1 and rbbpi/lbp2, respectively, which were identified by expressed sequence tag (est) analysis of a lipopolysaccharide (lps)-stimulated rock bream liver cdna library. the two cdna displayed 36.9% identity at the translated amino acid level. despite the low level of identity between the two genes, high conservation was seen in the bpi/lbp/cetp n-terminal, lps-binding, the proline-rich central and the bpi/lbp/cetp c-terminal domains. the full-length ... | 2012 | 22521422 |
| a teleostean angiotensinogen from oplegnathus fasciatus responses to immune and injury challenges. | angiotensinogen (agt) is the precursor of the renin-angiotensin system and contributes to osmoregulation, acute-phase and immune responses. a full-length cdna of the agt (2004 bp with a 1389 bp coding region) was isolated from rock bream (rb), oplegnathus fasciatus. the encoded polypeptide of 463 amino acids had a predicted molecular mass of 51.6 kda. rbagt possessed a deduced signal peptide of 22 residues upstream of a putative angiotensin i sequence ((23)nrvyvhpfhl(32)). rbagt possessed a spec ... | 2012 | 22300787 |
| genomic structure and immunological response of an stat4 family member from rock bream (oplegnathus fasciatus). | the janus tyrosine kinase (jak)/signal transducer and activator of transcription (stat) signaling pathway plays a critical role in host defense against viral and bacterial infections. stat proteins are a group of transcription factors that translocate into the nucleus and are critical for the induction of many genes crucial for the allergic cascade and immune defense. in the present study, a member of the stat4 family was identified from rock bream (rbstat4) at the genomic level, and its transcr ... | 2013 | 24055510 |
| molecular cloning and expression analysis of interleukin (il)-15 and il-15 receptor α from rock bream, oplegnathus fasciatus. | mammalian interleukin (il)-15 plays an important role in the activation of cd8(+) t cells and natural killer (nk) cells along with its receptor α (il-15rα). to understand the potential roles of il-15 and il-15rα in fish, we identified il-15 and il-15rα cdna from rock bream (oplegnathus fasciatus) and investigated their gene expression profiles after bacterial and viral infection. coding regions of rock bream (rb) il-15 and rbil-15rα cdnas were 534 and 402 bp encoding 177 and 133 amino acid resid ... | 2013 | 23911652 |
| marine teleost ortholog of catalase from rock bream (oplegnathus fasciatus): molecular perspectives from genomic organization to enzymatic behavior with respect to its potent antioxidant properties. | catalases are well known antioxidant enzymes that can mainly dismutate hydrogen peroxide into water and oxygen in order to prevent oxidative stress. the complete genomic dna (gdna) sequence of the catalase gene from rock bream (oplegnathus fasciatus) was identified from our custom-constructed bac genomic dna library and designated as rbcat. rbcat consists of 13 exons, separated by 12 introns, within a 13,722-bp gdna sequence. the complete cdna sequence (3303 bp) of rbcat is comprised of a 1581-b ... | 2013 | 23872475 |
| genomic characterization and transcriptional evidence for the involvement of complement component 7 in immune response of rock bream (oplegnathus fasciatus). | the complement component 7 (c7) is the central mediator of pathogenic attack at the membrane surface and its binding to the c5b-7 complex triggers cytolytic signaling. in this study, c7 of rock bream (oplegnathus fasciatus) was identified (rb-c7) and characterized at the genomic level. the rb-c7 gene contains 18 exons and 17 introns and is composed of a 2490 bp complete open reading frame (orf). the encoded polypeptide (830 amino acids) contains a number of well-conserved c7 signature domains. i ... | 2013 | 23603298 |
| molecular characterisation and biological activity of a novel cxc chemokine gene in rock bream (oplegnathus fasciatus). | chemokines are chemoattractant cytokines defined by the presence of four conserved cysteine residues. in mammals, these cytokines can be divided into four subfamilies depending on the arrangement of the first two conserved cysteines in the sequence, and include the cxc(α), cc(β), c(γ), and cx3c(δ) classes. we identified cxc chemokine cdna, designated rbcxc, isolated using expressed sequence tag analysis of a lipopolysaccharide (lps)-stimulated rock bream liver cdna library. the full-length rbcxc ... | 2013 | 23376472 |
| first molecular cloning and gene expression analysis of a teleost cd200 (ox-2) glycoprotein from rock bream, oplegnathus fasciatus. | cd200 plays an important role in delivering an immunoregulatory signal to the immune system through interaction with its receptor. however, cd200 has not been characterized and its function in teleosts is unknown. in this study, the rock bream (oplegnathus fasciatus) cd200 gene (rbcd200) was cloned and its expression profile was analyzed after infection with edwardsiella tarda, streptococcus iniae or red seabream iridovirus (rsiv). the coding region of rbcd200 cdna was 855 bp, encoding 284 amino ... | 2013 | 23128045 |
| three complement component 1q genes from rock bream, oplegnathus fasciatus: genome characterization and potential role in immune response against bacterial and viral infections. | complement component 1q (c1q) is a subcomponent of the c1 complex and the key protein that recognizes and binds to a broad range of immune and non-immune ligands to initiate the classical complement pathway. in the present study, we identified and characterized three novel c1q family members from rock bream, oplegnathus fasciatus. the full-length cdnas of c1q a-like (rbc1qal), c1q b-like (rbc1qbl), and c1q c-like (rbc1qcl) consist of 780, 720 and 726 bp of nucleotide sequence encoding polypeptid ... | 2013 | 23994081 |
| a teleostean counterpart of ferritin m subunit from rock bream (oplegnathus fasciatus): an active constituent in iron chelation and dna protection against oxidative damage, with a modulated expression upon pathogen stress. | ferritins are biological iron chelators that can sequestrate excess iron to maintain iron homeostasis in the body. ferritins basically consist of 2 types of subunits, designated as h and l. however, another new subunit, ferritin "m" which possesses characteristic features of both the h and l subunits, was recently identified in lower vertebrates, mostly in fish. in this study, a ferritin m-like subunit from rock bream (oplegnathus fasciatus) (rbferm) was characterized at the molecular level, and ... | 2013 | 23978565 |
| genomic characterization, expression analysis, and antimicrobial function of a glyrichin homologue from rock bream, oplegnathus fasciatus. | antimicrobial peptides are important innate effector molecules, playing a vital role in antimicrobial immunity in all species. glyrichin is a transmembrane protein and an antibacterial peptide, exerting its functions against a wide range of pathogenic bacteria. in this study, cdna and a bac clone harboring the glyrichin gene were identified from rock bream and characterized. genomic characterization showed that the ofglyrichin gene exhibited a 3 exon-2 intron structure. ofglyrichin is a 79-amino ... | 2013 | 23968692 |
| akirin2 homologues from rock bream, oplegnathus fasciatus: genomic and molecular characterization and transcriptional expression analysis. | akirins are conserved nuclear resident nf-κb signaling pathway molecules. isoforms of akirins found in various organisms are known to play diverse roles. in this study, we have characterized two akirin2 homologues from rock bream, ofak2(1) and ofak2(2). the proteins derived from ofak2(1) and ofak2(2) revealed the presence of nuclear localization signal. multiple sequence alignment and pairwise alignment of ofak2(1) and ofak2(2) with the akirin homologues, revealed high conservation and identity. ... | 2013 | 23770624 |
| molecular characterisation and expression analysis of the cathepsin h gene from rock bream (oplegnathus fasciatus). | cathepsins are lysosomal cysteine proteases belonging to the papain family, whose members play important roles in normal metabolism for the maintenance of cellular homeostasis. rock bream (oplegnathus fasciatus) cathepsin h (rbctsh) cdnas were identified by expressed sequence tag analysis of a lipopolysaccharide-stimulated rock bream liver cdna library. the full-length rbctsh cdna (1326 bp) contained an open reading frame of 978 bp encoding 325 amino acids. the presence of an erfnin-like motif w ... | 2013 | 23629026 |
| fish lily type lectin-1 contains β-prism architecture: immunological characterization. | in this study we report a full-length lily type lectin-1 (csltl-1) identified from striped murrel, channa striatus. csltl-1 was identified from the established c. striatus cdna library using gs-flx™ genome sequencing technology and was found to contain 354 nucleotide base pairs and its open reading frame (orf) encodes a 118 amino acid residue. csltl-1 mrna is predominately expressed in the gills and is up-regulated upon infection with fungus (aphanomyces invadans) and bacteria (aeromonas hydroph ... | 2013 | 23911406 |
| chromosomal mapping of microsatellite repeats in the rock bream fish oplegnathus fasciatus, with emphasis of their distribution in the neo-y chromosome. | despite the theoretical and experimental progress, our understanding on sex chromosome differentiation is still diagrammatic. the accumulation of repetitive dna sequences is believed to occur in early stages of such differentiation. as fish species present a wide range of sex chromosome systems they are excellent models to examine the differentiation of these chromosomes. in the present study, the chromosomal distribution of 9 mono-, di- and tri-nucleotide microsatellites were analyzed using flu ... | 2013 | 23510140 |
| characterization of mif family proteins: mif and ddt from rock bream, oplegnathus fasciatus. | macrophage migration inhibitory factor (mif) is a pleiotropic molecule playing vital roles in various signaling cascades, including cell proliferation, and activation of immune responses against infections. it is well known as a pivotal regulator of innate immunity. in this study, we have rescued and characterized two members of the mif family, macrophage migration inhibitory factor (ofmif) and d-dopachrome tautomerase (ofddt) from rock bream, oplegnathus fasciatus. the deduced ofmif and ofddt p ... | 2013 | 23688964 |
| a manganese superoxide dismutase with potent antioxidant activity identified from oplegnathus fasciatus: genomic structure and transcriptional characterization. | in this study, we describe the identification and characterization of manganese superoxide dismutase, an important antioxidant enzyme acting as the chief reactive oxygen species (ros) scavenger, from rock bream oplegnathus fasciatus (of-mmnsod) at genomic- and transcriptional-levels as well as the biological activity of recombinant protein. the of-mmnsod protein portrayed distinct mnsod family features including signature motifs, metal association sites and the typical active site topology. it w ... | 2013 | 23022055 |
| molecular identification and expression analysis of the cc chemokine gene in rock bream (oplegnathus fasciatus) and the biological activity of the recombinant protein. | we identified the cc chemokine cdna designated as rbcc1 (cc chemokine 1 in rock bream, oplegnathus fasciatus), which was isolated using expressed sequence tag (est) analysis of a lipopolysaccharide (lps)-stimulated rock bream liver cdna library. the full-length rbcc1 cdna (850 bp) contained an open reading frame (orf) of 366 bp encoding 122 amino acids. results from our phylogenetic analysis demonstrated that the rbcc1 was closest relationship to the orange-spotted grouper and mi-iyu croaker cc ... | 2013 | 23357024 |
| genomic characterization and expression analysis of complement component 8α and 8β in rock bream (oplegnathus fasciatus). | the complement component 8α and 8β are glycoproteins that mediate formation of the membrane attack complex (mac) on the surface of target cells. full-length complement c8α (rb-c8α) and c8β (rb-c8β) sequences were identified from a cdna library of rock bream (oplegnathus fasciatus), and their genomic sequences were obtained by screening and sequencing of a bacterial artificial chromosome (bac) genomic dna library of rock bream. the rb-c8α gene contains 64bp of 5'-utr, open reading frame (orf) of ... | 2013 | 23059376 |
| isolation and characterization of male-specific dna markers in the rock bream oplegnathus fasciatus. | sex-specific dna markers applicable were very useful for elucidating the sex-determination mechanism and sex control in fishes. in the present study, amplified fragment-length polymorphism (aflp) approach with 144 primer combinations was employed to identify sex-specific markers in the rock bream. four male-specific aflp fragments were identified which were designated as opl286, opl237, opl422, and opl228. further sequence analysis of the sex markers' genomic region revealed subtle differences b ... | 2013 | 22855400 |
| development and characterization of novel microsatellite markers in the rock bream fish oplegnathus fasciatus. | the rock bream fish oplegnathus fasciatus is one of the most popular aquaculture species in china. in the present study, 15 novel polymorphic microsatellite loci were isolated and characterized from a wild population of o. fasciatus from the zhoushan coast of china. the number of alleles per polymorphic locus ranged from 4 to 9 in a sample of 30 individuals. observed and expected heterozygosities per locus varied from 0.267 to 0.767 and from 0.395 to 0.859, respectively. eleven of the 15 microsa ... | 2013 | 24390995 |
| induced morphological changes in larval rock bream, oplegnathus fasciatus, under starvation. | morphological changes in the reared rock bream, oplegnathus fasciatus, from hatching to six days after hatching were examined during the early growth stage under starvation. all the larvae died within five days when feeding was delayed for three days after hatching. these results imply that initial larval food should be supplied within two days of hatching. changes in the pectoral angle and the ratios of eye height to head height, gut height to standard length, and gut height to myotome height i ... | 2013 | 25949156 |
| genomic structural characterization and transcriptional expression analysis of proteasome activator pa28α and pa28β subunits from oplegnathus fasciatus. | proteasomes are multicatalytic subunit complexes involved in the degradation of cytosolic proteins and antigen presentation. in this study, we have characterized the alpha and beta subunits of proteasome activator complex from rock bream at the molecular level. rbpa28α and rbpa28β possessed the characteristic features of the subunits identified from mammals and teleosts. the rbpa28α and rbpa28β proteasome subunits contained a proline-rich motif (region a), subunit-specific insert in the region c ... | 2013 | 23916540 |
| status and future perspectives of vaccines for industrialised fin-fish farming. | fin fish farming is developing from extensive to intensive high industrial scale production. production of fish in high-density growth conditions requires effective vaccines in order to control persistent and emerging diseases. vaccines can also have significant positive impact on the reduced usage of antibiotics. this was demonstrated when vaccines were introduced in norway for atlantic salmon (salmo salar) in the late eighties and early nineties, resulting in a rapid decline of antibiotics con ... | 2013 | 23769873 |
| recombinant thioredoxin 1 protein: the immune-adjuvant effect of streptococcus iniae and its safety in rock bream, oplegnathus fasciatus. | adjuvant is an immune enhancer commonly used during vaccination to enhance the host immune response. in the present study, we analysed the recombinant protein produced from rock bream thioredoxin 1 cdna (rrbtrx1). to verify the immune-stimulatory effect of this recombinant protein, changes in the expression level of several genes were investigated using the cdna microarray chips in rock bream peripheral blood leukocytes stimulated with rrbtrx1. furthermore, the immune responses of rock bream to ... | 2014 | 24830770 |
| rock bream (oplegnathus fasciatus) il-12p40: identification, expression, and effect on bacterial infection. | il-12p40, also called il-12β, is a subunit of the proinflammatory cytokines interleukin (il)-12 and il-23. in teleost, il-12p40 homologues have been identified in several species, however, the biological function of fish il-12p40 is essentially unknown. in this work, we reported the identification and analysis of an il-12p40, ofil-12p40, from rock bream (oplegnathus fasciatus). ofil-12p40 is composed of 361 amino acids and possesses a conserved il-12p40 domain and a wsxws signature motif charact ... | 2014 | 24875010 |
| molecular characterization and expressional affirmation of the beta proteasome subunit cluster in rock bream immune defense. | immunoproteasomes are primarily induced upon infection and formed by replacing constitutive beta subunits with inducible beta subunits which possess specific cleavage properties that aid in the release of peptides necessary for mhc class i antigen presentation. in this study, we report the molecular characterization and expression analysis of the inducible immunosubunits psmb8, psmb9, psmb9-l, and psmb10 from rock bream, oplegnathus fasciatus. the three subunits shared common active site residue ... | 2014 | 24867079 |
| genomic characterization of interferon regulatory factor 5 from rock bream (oplegnathus fasciatus) and its role in antiviral defense. | the interferon regulatory factor 5 (irf5) is a key mediator of the toll-like receptor (tlr)7 and tlr8 signaling pathways. in this study, we describe the identification of irf5 (rb-irf5) from rock bream fish (oplegnathus fasciatus) and its characteristics features at the genomic and expression levels. the full-length rb-irf5 sequence was identified from a cdna library and its genomic sequence was obtained by screening and sequencing of a bacterial artificial chromosome (bac) genomic dna library o ... | 2014 | 24560681 |
| rock bream (oplegnathus fasciatus) viperin is a virus-responsive protein that modulates innate immunity and promotes resistance against megalocytivirus infection. | viperin in mammals is known to be an antiviral protein that inhibits the replication of diverse dna and rna viruses. in teleost, viperin homologues have been identified in a large number of species and, in some cases, are stimulated in transcription by viruses. however, the biological significance of fish viperin protein in antiviral immunity has not been investigated. in this study, we identified a viperin homologue from rock bream (oplegnathus fasciatus) (named ofvip) and examined its expressi ... | 2014 | 24525178 |
| functional characterization of the evolutionarily preserved mitochondrial antiviral signaling protein (mavs) from rock bream, oplegnathus fasciatus. | antimicrobial immune defense is evolutionarily preserved in all organisms. mammals have developed robust, protein-based antiviral defenses, which are under constant investigation. studies have provided evidences for the various fish immune factors sharing similarity with those of mammals. in this study, we have identified an ortholog of mitochondrial antiviral signaling protein from rock bream, oplegnathus fasciatus. rbmavs cdna possesses an open reading frame (orf) of 1758 bp coding for a prote ... | 2014 | 25107693 |
| characterization of rock bream (oplegnathus fasciatus) cytosolic cu/zn superoxide dismutase in terms of molecular structure, genomic arrangement, stress-induced mrna expression and antioxidant function. | superoxide dismutases (sods) are dedicated to scavenge and dismutate the superoxide anions in order to protect the cells from oxidative stress by establishing the redox homeostasis. in this study, we describe a cytosolic cu/znsod, the second sod member from rock bream oplegnathus fasciatus (of-ccu/znsod) at molecular, genomic structural-, transcriptional- and functional-levels. the determination of genomic arrangement of of-ccu/znsod by means of a bac library revealed that its primary transcript ... | 2014 | 25088251 |
| molecular profile and functional characterization of the ferritin h subunit from rock bream (oplegnathus fasciatus), revealing its putative role in host antioxidant and immune defense. | ferritins are iron binding proteins made out of 24 subunits, involved in iron homeostasis and metabolism in cellular environments. here, we sought to identify and functionally characterize a one type of subunits of ferritin (ferritin h-like subunit) from rock bream (oplegnathus fasciatus; rbferh). the complete coding sequence of rbferh was 531 bp in length, encoding a 177-amino acid protein with a predicted molecular mass of 20.8 kda. the deduced protein structure possessed the domain architectu ... | 2014 | 25020197 |
| quantitative real time rt-pcr study of pathogen-induced gene expression in rock bream (oplegnathus fasciatus): internal controls for data normalization. | rock bream oplegnathus fasciatus is an important economic fish species. in this study, we evaluated the appropriateness of six housekeeping genes as internal controls for quantitative real-time pcr (rt-qpcr) analysis of gene expression in rock bream before and after pathogen infection. the expression of the selected genes in eight tissues infected with vibrio alginolyticus or megalocytivirus was determined by rt-qpcr, and the pcr data were analyzed with genorm and normfinder algorithms. the resu ... | 2014 | 24657097 |
| comparative genomic of the teleost cathepsin b and h and involvement in bacterial induced immunity of miiuy croaker. | cathepsins are a family of lysosomal proteases play different roles at physiological and pathological states and present in almost all animals as well as other organisms. cathepsins b and h are both cysteine proteases of cathepsins. cathepsin b and h have been studied playing parts in protein degradation/turnover, antigen presentation/processing and hormone maturation in mammals. however, little is known about the structures and functions of cathepsin b and h in teleosts. in the present study, w ... | 2014 | 25181651 |
| gene expression of pro- and anti-apoptotic proteins in rock bream (oplegnathus fasciatus) infected with megalocytivirus (family iridoviridae). | viruses belonging to the genus megalocytivirus cause diseases in marine fishes primarily in east and southeast asian countries. rock bream iridovirus (rbiv), which is a member of the megalocytivirus genus, causes severe mass mortalities in rock beam (oplegnathus fasciatus) in korea. in this study, we assessed apoptosis-related gene expression patterns in megalocytivirus-infected rock bream in high mortality and low mortality conditions to determine important apoptosis-related factors, which may ... | 2014 | 24463468 |
| potential for a live red seabream iridovirus (rsiv) vaccine in rock bream oplegnathus fasciatus at a low rearing temperature. | serious economic losses have occurred in fingerlings and market-sized rock bream oplegnathus fasciatus in korea due to red seabream iridovirus (rsiv) infection. we demonstrated previously that viral multiplication in fish is downregulated by maintaining fish at far from optimum temperatures at the onset of disease. we applied this concept to develop a live rsiv vaccine in rock bream. mortalities in rock bream that were inoculated with rsiv and reared at 21-30°c were ≥90%, whereas no mortality wa ... | 2014 | 24252702 |
| modulation of proteome expression by f-type lectin during viral hemorrhagic septicemia virus infection in fathead minnow cells. | lectins found in fish tissues play an important role in the innate immune response against viral infection. a fucose-binding type lectin, rbftl-3, from rock bream (oplegnathus fasciatus) was identified using expressed sequence tag (est) analysis. the expression of rbftl-3 mrna was higher in intestine than other tissues of rock bream. to determine the function of rbftl-3, vhsv-susceptible fathead minnow (fhm) cells were transfected with pcdna3.1(+) or pcdna3.1(+)-rbftl-3 and further infected with ... | 2014 | 24931624 |
| genomic identification and molecular characterization of a non-mammalian tnfaip8l2 gene from oplegnathus fasciatus. | tumor necrosis factor alpha-induced protein 8-like 2 (tnfaip8l2) is a newly described negative immune regulator, whose enigmatic biological functions are not clearly understood. in the present study, the tnfaip8l2 homolog of rock bream (oplegnathus fasciatus) was identified and characterized. the genomic composition of rock bream tnfaip8l2 (~6.7 kb) represented a tripartite arrangement in which three exons are interrupted by two introns. the rock bream tnfaip8l2 transcript (1974 bp) possessed a ... | 2014 | 24583177 |
| a c1 inhibitor ortholog from rock bream (oplegnathus fasciatus): molecular perspectives of a central regulator in terms of its genomic arrangement, transcriptional profiles and anti-protease activities of recombinant peptide. | c1 inhibitor (c1inh), a member of serpin superfamily, is a crucial regulator of the activation of various plasmatic cascades associated with immunity and inflammation. this study describes the identification and characterization of a c1inh gene from rock bream oplegnathus fasciatus (ofc1inh) at structural, expressional and functional levels. the cdna-(2245bp) and corresponding gdna-sequences (5.2kbp) of ofc1inh were isolated from rock bream transcriptome- and bac-libraries, respectively. predict ... | 2014 | 24056051 |
| a novel murrel channa striatus mitochondrial manganese superoxide dismutase: gene silencing, sod activity, superoxide anion production and expression. | we have reported the molecular characterization including gene silencing, superoxide activity, superoxide anion production, gene expression and molecular characterization of a mitochondrial manganese superoxide dismutase (mmnsod) from striped murrel channa striatus (named as csmmnsod). the csmmnsod polypeptide contains 225 amino acids with a molecular weight of 25 kda and a theoretical isoelectric point of 8.3. in the n-terminal region, csmmnsod carries a mitochondrial targeting sequence and a s ... | 2014 | 25183231 |
| immunological role of c4 cc chemokine-1 from snakehead murrel channa striatus. | in this study, we have reported a cdna sequence of c4 cc chemokine identified from snakehead murrel (also known as striped murrel) channa striatus (named as cscc-chem-1) normalized cdna library constructed by genome sequencing flx™ technology (gs-flx™). cscc-chem-1 is 641 base pairs (bp) long that contain 438 bp open reading frame (orf). the orf encodes a polypeptide of 146 amino acids with a molecular mass of 15 kda. the polypeptide contains a small cytokine domain at 30-88. the domain carries ... | 2014 | 24231766 |
| cloning, characterisation, and expression analysis of the cathepsin d gene from rock bream (oplegnathus fasciatus). | cathepsins are lysosomal cysteine proteases belonging to the papain family, members of which play important roles in normal metabolism for the maintenance of cellular homeostasis. rock bream (oplegnathus fasciatus) cathepsin d (rbctsd) cdnas were identified by expressed sequence tag analysis of a lipopolysaccharide-stimulated rock bream liver cdna library. the full-length rbctsd cdna (1644 bp) contained an open reading frame of 1191 bp encoding 396 amino acids. alignment analysis revealed that t ... | 2014 | 25038285 |
| two nf-κb inhibitor-alpha (iκbα) genes from rock bream (oplegnathus fasciatus): molecular characterization, genomic organization and mrna expression analysis after immune stimulation. | ikba is a member of ikb family, which sequesters nf-kb in an inactivate form in the cytoplasm and blocks the translocation of nf-kb to nucleus. the ikba paralogs of rock bream (ofikba-a and ofikba-b) encoded ikba proteins with typical features including, highly conserved ikb degradation motif, six ankyrin repeats and a pest sequence. however, their amino acid identity and similarity were only 55.6 and 69.7%, respectively suggesting that these two genes could be the two different isoforms of ikba ... | 2014 | 25462458 |
| two carboxypeptidase counterparts from rock bream (oplegnathus fasciatus): molecular characterization, genomic arrangement and immune responses upon pathogenic stresses. | carboxypeptidases (cps) are proteases that hydrolyze c-terminal peptide bonds. they are involved in regulating the complement system of the immune system. here, we report the molecular characterization and immune response of two carboxypeptidases, named carboxypeptidase a (rb-cpa) and carboxypeptidase n1 (rb-cpn1), from rock bream. the genomic sequence of rb-cpa contains 12 exons interrupted by 11 introns, while the genomic sequence of rb-cpn1 has 9 exons and 8 introns. the cdna sequence of rb-c ... | 2014 | 25457548 |
| molecular cloning and expression analysis of two lipopolysaccharide-induced tnf-α factors (litafs) from rock bream, oplegnathus fasciatus. | lipopolysaccharide (lps)-induced tumor necrosis factor (tnf)-α factor (litaf) plays an important role controlling the expression of tnf-α and the other cytokine genes in the presence of lps. however, two litaf homologues have not been characterized in fish. in this study, we cloned two distinct litaf (rblitaf1 and rblitaf2) cdnas from rock bream (oplegnathus fasciatus) and characterized their expression profiles after infection with edwardsiella tarda, streptococcus iniae or red seabream iridovi ... | 2014 | 24394623 |
| molecular characterization and transcriptional analysis of non-mammalian type toll like receptor (tlr21) from rock bream (oplegnathus fasciatus). | toll-like receptors (tlrs) are a large family of pattern recognition receptors, which are involved in triggering host immune responses against various pathogens by detecting their evolutionarily conserved pathogen associated molecular patterns (pamps). tlr21 is a non-mammalian type tlr, which recognizes unmethylated cpg dna, and is considered as a functional homolog of mammalian tlr9. in this study, we attempted to identify and characterize a novel tlr21 counterpart from rock bream (oplegnathus ... | 2014 | 25300254 |
| expression analysis and biological activity of moronecidin from rock bream, oplegnathus fasciatus. | the piscidin-family, one of antimicrobial peptides (amps) mainly distributed in fish, is crucial effectors of fish innate immune response. piscidin-family typically has broad-spectrum antimicrobial activity and the ability to modulate the immune response. in this study, we identified moronecidin (rbmoro) included in piscidin-family from rock bream and investigated its gene expression using quantitative real-time pcr and biological activity (including antimicrobial and cytotoxic activity). the co ... | 2014 | 25050859 |
| molecular aspects, genomic arrangement and immune responsive mrna expression profiles of two cxc chemokine receptor homologs (cxcr1 and cxcr2) from rock bream, oplegnathus fasciatus. | the cxcr1 and cxcr2 are the prototypical receptors and are the only known receptors for mammalian elr+ (glu-leu-arg) cxc chemokines, including cxcl8 (interleukin 8). these receptors transduce the elr+ chemokine signals and operate the downstream signaling pathways in inflammation and innate immunity. in this study, we report the identification and characterization of cxcr1 and cxcr2 genes from rock bream fish (ofcxcr1 and ofcxcr2) at the molecular level. the cdna and genomic dna sequences of the ... | 2014 | 24945570 |
| molecular genomic- and transcriptional-aspects of a teleost traf6 homolog: possible involvement in immune responses of oplegnathus fasciatus against pathogens. | tumor necrosis factor receptor (tnfr)-associated factor 6 (traf6) is a crucial docking molecule for tnfr superfamily and interleukin-1 receptor/toll-like receptor (il-1r/tlr) superfamily. as an adaptor protein in pathogen-induced signaling cascades, traf6 modulates both adaptive- and innate-immunity. in order to understand the immune responses of teleost traf6, oplegnathus fasciatus traf6-like gene (oftraf6) was identified and characterized. genomic length of oftraf6 (4 kb), obtained by means of ... | 2015 | 25449707 |
| first molecular cloning and gene expression analysis of teleost cd42 (glycoprotein ib beta chain) gpib-ix-v subunit from rock bream, oplegnathus fasciatus. | cd42 is a platelet membrane glycoprotein ib that plays a key role in haemostasis and thrombin-induced platelet activation. here, we report the molecular cloning and sequence analysis of the cd42c gene from rock bream (oplegnathus fasciatus). rock bream cd42 (rbcd42c) gene expression profiles were determined after infection with streptococcus iniae, edwardsiella tarda and red seabream iridovirus (rsiv). the full-length rbcd42c cdna contained an open reading frame of 624 bp encoding 207 amino acid ... | 2015 | 25445900 |
| gene expression and functional characterization of serum amyloid p component 2 in rock bream, oplegnathus fasciatus. | mammalian serum amyloid p component (sap) recognizes a wide range of exogenous pathogenic substances and activates a complementary pathway leading to pathogen clearance. to determine the potential roles of sap in the fish immune system, sap (rbsap2) gene was cloned from ests analysis of rock bream (oplegnathus fasciatus), which consisted of a signal peptide and pentraxin domain. phylogenetic analysis revealed that the rbsap2 gene was classified with other known fish saps. rbsap2 was highly expre ... | 2015 | 26455663 |
| gene structure, molecular characterization and transcriptional expression of two p38 isoforms (mapk11 and mapk14) from rock bream (oplegnathus fasciatus). | the p38 kinases are one of the four subgroups of mitogen-activated protein kinase (mapk) superfamily which are involved in the innate immunity. the p38 subfamily that includes four members namely p38α (mapk14), p38β (mapk11), p38γ (mapk12) and p38δ (mapk13), regulates the activation of several transcription factors. in this study, a p38β (ofmapk11) homolog and a p38α (ofmapk14) homolog of oplegnathus fasciatus were identified at genomic level. results clearly showed that both mapk11 and mapk14 a ... | 2015 | 26363230 |
| characterization of rock bream (oplegnathus fasciatus) complement components c1r and c1s in terms of molecular aspects, genomic modulation, and immune responsive transcriptional profiles following bacterial and viral pathogen exposure. | the complement components c1r and c1s play a crucial role in innate immunity via activation of the classical complement cascade system. as initiators of the pathogen-induced signaling cascade, c1r and c1s modulate innate immunity. in order to understand the immune responses of teleost c1r and c1s, oplegnathus fasciatus c1r and c1s genes (ofc1r and ofc1s) were identified and characterized. the genomic sequence of ofc1r was enclosed with thirteen exons that represented a putative peptide with 704 ... | 2015 | 26241508 |
| a homolog of kunitz-type serine protease inhibitor from rock bream, oplegnathus fasciatus: molecular insights and transcriptional modulation in response to microbial and pamp stimulation, and tissue injury. | serine proteases and their inhibitors play vital roles in diverse biological processes. in this study, we identified and characterized cdna coding for a kunitz-type serine protease inhibitor (spi), which we designated as rbkspi, in a commercially important species, rock bream. the full-length cdna sequence of rbkspi consisted of 2452 bp with an open reading frame (orf) of 1521 bp encoding a polypeptide of 507 amino acid (aa) residues. in the rbkspi protein, manec, pkd, ldla, and two kunitz domai ... | 2015 | 26162478 |