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transcriptome profiling of two asparagus bean (vigna unguiculata subsp. sesquipedalis) cultivars differing in chilling tolerance under cold stress.cowpea (v. unguiculata l. walp.) is an important tropical grain legume. asparagus bean (v. unguiculata ssp. sesquipedialis) is a distinctive subspecies of cowpea, which is considered one of the top ten asian vegetables. it can be adapted to a wide range of environmental stimuli such as drought and heat. nevertheless, it is an extremely cold-sensitive tropical species. improvement of chilling tolerance in asparagus bean may significantly increase its production and prolong its supply. however, ge ...026954786
[peculiarities of circadian rhythms in plants from different geographical latitudes].1 two species of plants (taraxacum arcticum and arnica angustifolia), collected in spitsbergen (geogr. latitude 76-80 degrees) exhibit endogenous circadian leaf movements but also movements with shorter periods. astragalus frigidus, a. alpinus and hedysarum hedysaroides, collected in arctic regions of continental europe, also show endogenous diurnal leaf movements. 2. in most of the species tested, there was no difference in the length of the free running periods of plants from arctic and centra ...196624557984
variation of nicotinamide adenine dinucleotide phosphate level in bean hypocotyls in relation to o(2) concentration.slices of hypocotyls from 3-day-old seedlings of vigna sesquipedalis (l.) fruwirth in the germination stage were incubated under various gaseous conditions. the nadp+nadph level in the hypocotyl slices changed with the oxygen tension. a high nadp+nadph level was observed under aerobic conditions and a low nadp+nadph level under anaerobic conditions.the 100 x nadh/nad+nadh ratio increased greatly under anaerobic conditions. in general a low nadp + nadph level corresponded with a high 100 x nadh/n ...196616656283
modification of metabolic pattern by variation of nicotinamide adenine dinucleotide phosphate level.the experiments were designed to get some information on the metabolism controlled by variation of the nadp level, which is known to change with the variation of environmental factors.the exogenous nadp added to the mitochondria prepared from vigna sesquipedalis cotyledons was associated with and/or penetrated into the mitochondria. the combined nadp served in the operation of the mitochondrial nadp-isocitric acid dehydrogenase.the variation of nadp level by exogenous nadp was observed to modify ...196916657076
relative activities of nad- and nadp- isocritric dehydrogenases in bean mitochondria modified by glycerol or nadp.mitochondria from cotyledons of vigna sesquipedalis (l.) fruwirth (starchy seed) showed no nad-isocitric dehydrogenase (nad-idh) activity by the methods which have been known to be useful for the detection of nad-idh in mitochondria of plants including castor bean and alaska pea. when the vigna cotyledon mitochondria were treated with glycerol, nad-idh activity appeared and nadp-isocitric dehydrogenase (nadp-idh) activity was inhibited. the inhibition of mitochondrial nadp-idh by glycerol was ov ...19694388200
isolation of lectins of different specificities on a single affinity adsorbent.affinity chromatography on sepharose-fetuin columns was used in a single step procedure to isolate the lectins concanavalin a, favin, phytohemagglutinin, wheat germ agglutinin, and limulus hemagglutinin. new lectins with unknown binding specificities were also purified by the same procedure from extracts of small california white beans, idaho red beans, and white pea beans. the purified lectins exhibited different cell surface mapping properties on erythrocytes, lymphocytes, and sperm cells. it ...19751165252
fatty acid composition of rhizobium spp.the fatty acid composition of 42 isolates belonging to the major plant affinity groups of rhizobium has been determined and found to vary reproducible with culture age. numerical taxonomic techniques applied to the 15 major fatty acid components of log-phase cultures of comparable physiological age showed that the rhizobia constitute a uniform group. however, two clusters comprising soybean-cowpea isolates and pea-bean isolates were evident. these observations, based on a simple analysis of only ...1979427657
effect of gamma irradiation on nutritional value of dry field beans (phaseolus vulgaris) for chicks.the effect of gamma irradiation (60co) of different varieties and breeding lines of dry field beans (phaseolus vulgaris) on chick growth was determined using a chick growth assay in which the diet contained approximately 50% beans. total protein (n x 6.25) in beans was not changed appreciably by irradiation (21 mrad) but protein solubility in water was decreased. irradiation increased in vitro enzymatic digestibility of bean protein by pepsin and by a mixture of trypsin, chymotrypsin and peptida ...1979448472
inheritance of seed protein content and other agronomic characters in long bean (vigna sesquipedalis fruw.).seven varieties of long bean, which included three local and four exotic, were crossed in a complete diallel. this was an attempt to study the inheritance of crude protein content, protein yield, flowering date, pod yield and yield components.both additive and non-additive gene effects were responsible for the genetic variation in the diallel population. however, dominance variance was more important than additive variance in crude protein content, number of pods per plant and number of seeds pe ...198024305859
glycemic responses to foods: possible differences between insulin-dependent and noninsulin-dependent diabetics.the effect on the blood glucose response of varying the amount (25 or 50 g) and type (bread or beans) of carbohydrate (ch2o) in test meals and of adding fat and protein was examined in a group of insulin dependent and noninsulin dependent diabetic volunteers. with noninsulin-dependent diabetics, the blood glucose area after a half bread portion was 48% that of the full bread meal (p less than 0.001). white pea beans (50 g ch2o) gave a blood glucose response of 41 +/- 5% (p less than 0.001) that ...19846496392
novel application of quantitative immunoassays for screening seed globulins of cowpea varieties.using antibodies raised in rabbits, radioimmunoassay (ria) and enzyme-linked immunosorbent assay (elisa) are standardized for cowpea (var. pusa barsati) seed globulins. the ria, when used to screen three stages of seed development, reveals that maximum globulins are detected at 28 days after flowering. when three different varieties of cowpea are assayed for their globulin content by ria and elisa, it is observed that the bold grain cowpea has the highest amount of related globulin as compared t ...19854063471
fluorescent banding pattern analysis of eight taxa of phaseolus and vigna in relation to their phylogenetic relationships.phylogenetic relationships among eight taxa of seven species of phaseolus and vigna (phaseolus angularis, p. aureus, p. calcaratus, p. coccineus, p. vulgaris, vigna sesquipedalis and v. sinensis; 2n = 22 each) were studied by the fluorescent chromosome banding technique. preparations of somatic metaphase chromosomes of each taxon were sequentially stained with giemsa, gc-specific fluorochrome chromomycin a3 (cma) and at-specific fluorochrome 4'-6-diamidino-2-phenylindole (dapi). on the basis of ...199324190189
agrobacterium mediated transformation of vigna sesquipedalis koern (asparagus bean).agrobacterium mediated transformation of vigna sesquipedalis was achieved using cotyledonary node explants prepared from 5 days old seedlings germinated on b5 basal medium, and transformed using agrobacterium tumefaciens strain eha101, carrying the phosphinothricin-n-acetyltransferase gene and neomycin-3-phosphotransferase-ii gene as selectable markers and gus gene as a screenable marker. gene transfer was achieved by inoculation of cotyledonary node explants with a bacterial suspension and a fu ...200011272416
use of cc traps with different trap base colors for silverleaf whiteflies (homoptera: aleyrodidae), thrips (thysanoptera: thripidae), and leafhoppers (homoptera: cicadellidae).during 1996, 1997, and 1999, studies were conducted in cotton, sugar beets, alfalfa, yardlong bean, and peanut fields to compare insect catches in cc traps equipped with different trap base colors. the studies were conducted in southwestern united states, china, and india. the nine colors, white, rum, red, yellow, lime green, spring green, woodland green (dark green), true blue, and black, varied in spectral reflectance in the visible (400-700 nm) and near-infrared (700-1050 nm) portions of spec ...200010985050
false nitric oxide synthase immunoreactivity in asparagus bean (vigna sesquipdalis) 200010835298
diversity among bradyrhizobium isolates nodulating yardlong bean and sunnhemp in guam.to isolate and characterize bradyrhizobia that nodulate yardlong bean and sunnhemp in guam.200212234340
bean common mosaic virus isolates causing different symptoms in asparagus bean in china differ greatly in the 5'-parts of their genomes.potyvirus isolates from asparagus bean ( vigna sesquipedalis) plants in zhejiang province, china, caused either rugose and vein banding mosaic symptoms (isolate r) or severe yellowing (isolate y) in this host, but were otherwise similar in host range. both isolates were completely sequenced and shown to be isolates of bean common mosaic virus (bcmv). the complete sequences were 9992 (r) or 10062 (y) nucleotides long and shared 91.7% identical nucleotides (93.2% identical amino acids) in their ge ...200212111434
purification of a trypsin-stable lectin with antiproliferative and hiv-1 reverse transcriptase inhibitory activity.a lectin, with a molecular mass of approximately 60 kda and two different subunits exhibiting an n-terminal sequence manifesting considerable homology to phytohemagglutinin from phaseolus species, was isolated from the ground bean (vigna sesquipedalis cv ground bean). the lectin was unique in hemagglutinating activity was inhibited by polygalacturonic acid and not by galacturonic acid and other simple monosaccharides. the lectin was isolated by affinity chromatography on affi-gel blue gel, ion e ...200312565897
sesquin, a potent defensin-like antimicrobial peptide from ground beans with inhibitory activities toward tumor cells and hiv-1 reverse transcriptase.an antifungal peptide with a molecular mass around 7 kda and an n-terminal sequence highly homologous to defensin was isolated from ground beans (vigna sesquipedalis cv. 'ground bean'). the peptide was adsorbed on affi-gel blue gel and on mono s. it exerted an antifungal action on botrytis cinerea, fusarium oxysporum and mycosphaerella arachidicola; and an antibacterial action on escherichia coli b, proteus vulgaris, mycobacterium phlei and bacillus megaterium. the antimicrobial activity was inh ...200515949629
pneumatosis cystoides intestinalis in a rabbit doe (oryctolagus cuniculus).pneumatosis cystoides intestinalis (pci) is an infrequent condition of animals characterized by the existence of numerous thin-walled, gas-filled cystic structures within the intestinal wall and adjacent lymph nodes. microscopically, the cystic structures appear to be dilated lymphatics located in the lamina propria, submucosa, muscularis, subserosa, mesentery, and mesenteric lymph nodes. this report describes a case of pneumatosis cystoides intestinalis in a rabbit doe from an organic farm wher ...200515945383
heavy metal accumulations of 24 asparagus bean cultivars grown in soil contaminated with cd alone and with multiple metals (cd, pb, and zn).crops grown in heavy metal contaminated soils are an important avenue for these toxic pollutants entering the human food chain. information on how crops respond to soil contaminations of single versus multiple metals is scarce and much needed. this study investigated the accumulation of cd by 24 cultivars of asparagus bean (vigna unguiculata subsp. sesquipedalis l., family fabaceae) under a low level (0.8 mg kg-1) and a high level (11.8 mg kg-1) of cd exposure in a garden experiment, and that in ...200717263511
pcr-based detection and partial genome sequencing indicate high genetic diversity in bangladeshi begomoviruses and their whitefly vector, bemisia tabaci.the population diversity of bangladeshi begomoviruses and their vector, bemisia tabaci was analysed by pcr-based detection and partial genome sequencing. b. tabaci adults and plants expressing symptoms of virus infection were collected from locations representing diverse agro-ecological regions of the country. universal and species-specific primers were used to detect begomoviruses in seven crops (chilli, okra, papaya, pumpkin, sponge gourd, tomato and yardlong bean) and two common weeds (agerat ...200716927121
identification and characterization of anthocyanins in yard-long beans (vigna unguiculata ssp. sesquipedalis l.) by high-performance liquid chromatography with diode array detection and electrospray ionization/mass spectrometry (hplc-dad-esi/ms) analysis.anthocyanins play an important role in physiological functions related to human health. the objective of this study was to investigate the profiles of anthocyanins in the immature purple pods and black seeds of yard-long beans ( vigna unguiculata ssp. sesquipedalis l.) using high-performance liquid chromatography (hplc) with diode array detection and electrospray ionization/mass spectrometry (dad-esi/ms) analysis. the individual anthocyanins were identified by comparing their mass spectrometric ...201020121192
profile and functional properties of seed proteins from six pea (pisum sativum) genotypes.extractability, extractable protein compositions, technological-functional properties of pea (pisum sativum) proteins from six genotypes grown in serbia were investigated. also, the relationship between these characteristics was presented. investigated genotypes showed significant differences in storage protein content, composition and extractability. the ratio of vicilin:legumin concentrations, as well as the ratio of vicilin + convicilin: legumin concentrations were positively correlated with ...201021614186
a snp and ssr based genetic map of asparagus bean (vigna. unguiculata ssp. sesquipedialis) and comparison with the broader species.asparagus bean (vigna. unguiculata ssp. sesquipedialis) is a distinctive subspecies of cowpea [vigna. unguiculata (l.) walp.] that apparently originated in east asia and is characterized by extremely long and thin pods and an aggressive climbing growth habit. the crop is widely cultivated throughout asia for the production of immature pods known as 'long beans' or 'asparagus beans'. while the genome of cowpea ssp. unguiculata has been characterized recently by high-density genetic mapping and pa ...201121253606
flow injection assessment of nitrate contents in fresh and cooked fruits and vegetables grown in fiji.nitrates form part of the essential chemistry of soils and plants. thus, plant roots are able to absorb nitrate directly from the soil. it has been discovered that human nitrate intake is mainly from vegetables. vegetables play an important role in human nutrition since they are an outstanding source for vitamins, minerals, and biologically active compounds. in risk assessment of nitrate contents, this study reports the nitrate levels of 8 commonly consumed fruits and vegetables grown in fiji, t ...201122417577
phosphate solubilizers enhance npk fertilizer use efficiency in rice and legume cultivation.it has been reported that phosphate solubilizing bacteria (psb) are the most promising bacteria among the plant growth promoting rhizobacteria (pgpr); which may be used as biofertilizers for plant growth and nutrient use efficiency. moreover, these soil micro-organisms play a significant role in regulating the dynamics of organic matter decomposition and the availability of plant nutrients such as nitrogen (n), phosphorus (p), potassium (k) and other nutrients. through this study, the management ...201122558541
an ssr-based linkage map of yardlong bean (vigna unguiculata (l.) walp. subsp. unguiculata sesquipedalis group) and qtl analysis of pod length.yardlong bean (vigna unguiculata (l.) walp. subsp. unguiculata sesquipedalis group) (2n = 2x = 22) is one of the most important vegetable legumes of asia. the objectives of this study were to develop a genetic linkage map of yardlong bean using ssr makers from related vigna species and to identify qtls for pod length. the map was constructed from 226 simple sequence repeat (ssr) markers from cowpea (vigna unguiculata (l.) walp. subsp. unguiculata unguiculata group), azuki bean (vigna angularis ...201222242703
electrospinning-directed one-dimensional alignment of core-shell silica/polymer nanoparticles in nanofibers.nanofibers with ordered inorganic nanoparticles inside are great interesting for their potential applications in biomedical and photonics. in this work, yardlong-bean shaped nanofibers were fabricated by the combined technology of surface-initiated atom transfer radical polymerization (atrp) and coaxial electrospinning process. initially, the core-shell nanospheres were prepared by surface-initiated atrp of glycidyl methacrylate (gma) from silica nanoparticles with atrp initiators on surface. su ...201223035465
the genetics of domestication of yardlong bean, vigna unguiculata (l.) walp. ssp. unguiculata cv.-gr. sesquipedalis.the genetics of domestication of yardlong bean [vigna unguiculata (l.) walp. ssp. unguiculata cv.-gr. sesquipedalis] is of particular interest because the genome of this legume has experienced divergent domestication. initially, cowpea was domesticated from wild cowpea in africa; in asia a vegetable form of cowpea, yardlong bean, subsequently evolved from cowpea. information on the genetics of domestication-related traits would be useful for yardlong bean and cowpea breeding programmes, as well ...201222419763
first report of cowpea mild mottle carlavirus on yardlong bean (vigna unguiculata subsp. sesquipedalis) in venezuela.yardlong bean (vigna unguiculata subsp. sesquipedalis) plants with virus-like systemic mottling and leaf distortion were observed in both experimental and commercial fields in aragua state, venezuela. symptomatic leaves were shown to contain carlavirus-like particles. rt-pcr analysis with carlavirus-specific primers was positive in all tested samples. nucleotide sequences of the obtained amplicons showed 84%-74% similarity to corresponding sequences of cowpea mild mottle virus (cpmmv) isolates d ...201223242372
genome wide linkage disequilibrium in chinese asparagus bean (vigna. unguiculata ssp. sesquipedialis) germplasm: implications for domestication history and genome wide association studies.association mapping of important traits of crop plants relies on first understanding the extent and patterns of linkage disequilibrium (ld) in the particular germplasm being investigated. we characterize here the genetic diversity, population structure and genome wide ld patterns in a set of asparagus bean (vigna. unguiculata ssp. sesquipedialis) germplasm from china. a diverse collection of 99 asparagus bean and normal cowpea accessions were genotyped with 1127 expressed sequence tag-derived si ...201222378357
genotypic differences in pod wall and seed growth relate to invertase activities and assimilate transport pathways in asparagus bean.coordination of sugar transport and metabolism between developing seeds and their enclosing fruit tissues is little understood. in this study the physiological mechanism is examined using two genotypes of asparagus bean (vigna unguiculata ssp. sesquipedialis) differing in pod wall and seed growth rates. pod growth dominates over seed growth in genotype 'zhijiang 121' but not in 'zhijiang 282' in which a 'bulging pod' phenotype is apparent from 8 d post-anthesis (dpa) onward.201222447992
qtl mapping and epistatic interaction analysis in asparagus bean for several characterized and novel horticulturally important traits.asparagus bean (vigna. unguiculata. ssp sesquipedalis) is a subspecies and special vegetable type of cowpea (vigna. unguiculata l. walp.) important in asia. genetic basis of horticulturally important traits of asparagus bean is still poorly understood, hindering the utilization of targeted, dna marker-assisted breeding in this crop. here we report the identification of quantitative trait loci (qtls) and epistatic interactions for four horticultural traits, namely, days to first flowering (fld), ...201323375055
natural variation and gene regulatory basis for the responses of asparagus beans to soil drought.asparagus bean (vigna unguiculata ssp. sesquipedalis) is the asian subspecies of cowpea, a drought-resistant legume crop native to africa. in order to explore the genetic variation of drought responses in asparagus bean, we conducted multi-year phenotyping of drought resistance traits across the chinese asparagus bean mini-core. the phenotypic distribution indicated that the ssp. sesquipedalis subgene pool has maintained high natural variation in drought responses despite known domestic bottlene ...201526579145
a study on maruca vitrata infestation of yard-long beans (vigna unguiculata subspecies sesquipedalis).globally, maruca vitrata (geyer) is a serious yield constraint on food legumes including yard-long bean (vigna unguiculata subspecies sesquipedalis). however, there is a dearth of information on its damage potential, distribution and population dynamics in yard-long beans. in the present study, the level of m. vitrata larval infestation on flowers and pods of yard-long beans in sri lanka was determined with respect to three consecutive cropping seasons, yala, off and maha. results indicated that ...201527441212
genetic analysis of salt stress responses in asparagus bean (vigna unguiculata (l.) ssp. sesquipedalis verdc.).salt stress responses of 23 asparagus bean cultivars were evaluated using 14-day-old seedlings after 15-day exposure to 75 mm nacl in a hydroponics culture system. salt-induced changes in plant growth and morphology, photosynthetic capacity, cell membrane integrity, and cellular protection enzyme systems as well as other physiological and biochemical traits were investigated to identify genotypic variability in salt response. this study also analyzed heredity parameters and correlation of the sa ...201517956901
a rhizosphere-associated symbiont, photobacterium spp. strain meld1, and its targeted synergistic activity for phytoprotection against mercury.though heavy metal such as mercury is toxic to plants and microorganisms, the synergistic activity between them may offer benefit for surviving. in this study, a mercury-reducing bacterium, photobacterium spp. strain meld1, with an mic of 33 mg x kg(-1) mercury was isolated from a severely mercury and dioxin contaminated rhizosphere soil of reed (phragmites australis). while the whole genome sequencing of meld1 confirmed the presence of a mer operon, the mercury reductase mera gene showed 99% se ...201525816328
effect of addition of thermally modified cowpea protein on sensory acceptability and textural properties of wheat bread and sponge cake.this paper investigates the sensory acceptability and textural properties of leavened wheat bread and sponge cake fortified with cow protein isolates that had been denatured and glycated by thermal treatment. defatted cowpea flour was prepared from cow pea beans and the protein isolate was prepared (cpi) and thermally denatured (dcpi). to prepare glycated cowpea protein isolate (gcpi) the cowpea flour slurry was heat treated before isolation of the protein. cpi was more susceptible to thermal de ...201626471676
the joint toxicity of different temperature coefficient insecticides on apolygus lucorum (hemiptera: miridae).the effect of temperature on the cotoxicity coefficient (ctc) value was used to evaluate mixture efficacy of different temperature coefficient chemicals from 15 to 35°c by exposing third-instar apolygus lucorum (meyer-dür) to dip-treated asparagus bean pods. the results indicated the joint toxicity of same temperature coefficient insecticide (tci) types were unaffected by temperature. this means that even when temperatures change, the mixture ratios of the highest ctc values remained the same, a ...201627190041
silicon and nitrate differentially modulate the symbiotic performances of healthy and virus-infected bradyrhizobium-nodulated cowpea (vigna unguiculata), yardlong bean (v. unguiculata subsp. sesquipedalis) and mung bean (v. radiata).the effects of 2 mm silicon (si) and 10 mm kno₃ (n)-prime signals for plant resistance to pathogens-were analyzed in healthy and cowpea chlorotic mottle virus (ccmv) or cowpea mild mottle virus (cmmv)-infected bradyrhizobium-nodulated cowpea, yardlong bean and mung bean plants. in healthy plants of the three vigna taxa, nodulation and growth were promoted in the order of si + n > n > si > controls. in the case of healthy cowpea and yardlong bean, the addition of si and n decreased ureide and α-a ...201728914770
too much to handle? pesticide dependence of smallholder vegetable farmers in southeast asia.this study aimed to understand farmers' knowledge, attitudes, and practices regarding agricultural pest management and synthetic pesticide use in southeast asia. data were used from 900 farm households producing leaf mustard (brassica juncea (l.) czern. et coss.) and yard-long bean (vigna unguiculata subsp. sesquipedalis (l.) verdc.) in cambodia, laos and vietnam. farmers heavily depended on synthetic pesticides as their main method of pest control. most farmers were aware of the adverse health ...201728359998
effects of crop rotation and management system on water-extractable organic matter concentration, structure, and bioavailability in a chernozemic agricultural soil.water-extractable organic matter (weom) in soil affects contaminant mobility and toxicity, heterotrophic production, and nutrient cycling in terrestrial and aquatic ecosystems. this study focuses on the influences of land use history and agricultural management practices on the water extractability of organic matter and nutrients from soils. water-extractable organic matter was extracted from soils under different crop rotations (an annual rotation of wheat-pea/bean-wheat-flax or a perennial-bas ...201723673753
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