Publications

inhibitors of the arachidonate cascade from allium chinense and their effect on in vitro platelet aggregation.		1987	3677224
steroidal saponins from allium chinense and their inhibitory activities on cyclic amp phosphodiesterase and na+/k+ atpase.	the saponin fraction prepared from the methanolic extract of allium chinense bulbs exhibited inhibitory activities on cyclic amp phosphodiesterase (camp pde) (43.5%) and na+/k+ atpase (59.3%) at a sample concentration of 100 micrograms ml-1, respectively. attempted purification of the active fraction through column chromatography on silica gel and ods silica gel resulted in the isolation of six steroidal saponins, one of which appeared to be a new compound and one to be the first isolation from ...	1995	7492369
new furostanol glycosides, chinenoside iv and v, from allium chinense.	further studies on water-soluble components in the bulbs of allium chinense g. don have led to the isolation of two new furostanol saponins, chinenoside iv (1) and v (2). on the basis of chemical evidence and spectral analyses [1h-, 13c-nmr (dept), 1h-1h cosy (cosy45 and p-type), 1h-1h relay-cosy, 1h-13c cosy, hmbc, and fab-ms], the structure of 1 was established as 26-o-beta-glucopyranosyl-3 beta,26-dihydroxy-23-hydroxymethyl-25(r)- 5 alpha-furost-20(22)-en-6-one 3-o-beta-xylopyranosyl(1-->4)-[ ...	1996	8923813
furostanol glycosides from bulbs of allium chinense.	two new furostanol saponins, named chinenosides ii and iii, were isolated along with seven known compounds, from the bulbs of allium chinese g. don by a combination of silica gel, diaion hp-20, and octadecylsilanized (ods) silica gel column chromatographies and preparative hplc. on the basis of chemical and spectroscopic evidence, the structures of chinenosides ii and iii were determined to be 26-o-beta-glucopyranosyl 3 beta,26-dihydroxy-(25r)-5 alpha-furost-20(22)-en-6-one 3-o-beta-xylopyranosy ...	1996	8588870
[structural elucidation of the anticoagulation and anticancer constituents from allium chinense].	six compounds were isolated from the anticoagulation and anticancer fractions of the bulbs of allium chinense g. don. on the basis of chemical evidence and spectral analysis (ir, ei-ms, 1hnmr, 13cnmr, 1h-1h cosy, hmbc, hmqc and noesy), their structures were established as (25r, s)-5 alpha-spirostane-3 beta-ol 3-o-(beta-d-glucopyranosyl-(1-->2)-[beta-d-glucopyranosyl-(1-->3)]-beta-d- glucopyranosyl-(1-->4)-beta-d-galactopyranoside) (1), (25r, s)-5 alpha-spirostane-3 beta-ol 3-o-(beta-d-glucopyran ...	1998	12017003
saponins isolated from allium chinense g. don and antitumor-promoting activities of isoliquiritigenin and laxogenin from the same drug.	investigation of the chinese crude drug "xiebai," the bulbs of allium chinense g. don (liliaceae), led to the isolation of 2 saponins, xiebai-saponin i (laxogenin 3-o-beta-xylopyranosyl (1-->4)-[alpha-arabinopyranosyl (1-->6)-beta-glucopyranoside) (1) and laxogenin 3-o-alpha-arabinopyranosyl (1-->6)-beta-glucopyranoside (2), and the aglycone, laxogenin (3), together with 2 chalcones, isoliquiritigenin (4) and isoliquiritigenin-4-o-glucoside (5), and beta-sitosterol glucoside (6). compounds 1-5 w ...	2000	10823685
volatile constituents of chinese chive (allium tuberosum rottl. ex sprengel) and rakkyo (allium chinense g. don).	volatile components were isolated from chinese chive and rakkyo by simultaneous steam distillation-solvent extraction and analyzed by gc and gc-ms. sulfur compounds account for 88 and 94% of the total volatiles in the isolated extract of chinese chive and rakkyo, respectively. in addition to the sulfur compounds commonly reported in the genus allium, 27 novel volatile sulfur-containing components were found in the isolated extracts of both species. among them were a sulfide, disulfides, trisulfi ...	2001	11312859
characterisation of a potyvirus and a potexvirus from chinese scallion.	molecular analyses of viruses infecting chinese scallion (allium chinense g. don) showed that the plants did not contain any of the poty-, carla- or allexiviruses that are common in garlic plants in china. the complete sequences of a potyvirus and a potexvirus were determined and these were shown to represent different viruses from any in the databases. they could be transmitted mechanically to scallion but not to other allium species (including garlic) or to narcissus. the potyvirus, tentativel ...	2002	12038680
protective effects of steroids from allium chinense against h2o2-induced oxidative stress in rat cardiac h9c2 cells.	allium chinense, a traditional herbal medicine, has been used for the treatment of cardiovascular diseases for hundreds of years. in this study, a. chinense steroids (acss) including three steroidal glycosides and their parent aglycones were isolated from the bulbs of a. chinense. for the first time, their cardioprotective effects were evaluated in cultured rat cardiac h9c2 cells by pretreatment with acss for 24 h before exposure to 0.2 mm h(2)o(2). the results showed the cell viability decrease ...	2010	19653197
a genome walking strategy for the identification of nucleotide sequences adjacent to known regions.	to identify the transposon insertion sites in a soil actinomycete, saccharopolyspora spinosa, a genome walking approach, termed spta-pcr, was developed. in spta-pcr, a simple procedure consisting of ta cloning and a high stringency pcr, following the single primer-mediated, randomly-primed pcr, can eliminate non-target dna fragments and obtain target fragments specifically. using spta-pcr, the dna sequence adjacent to the highly conserved region of lectin coding gene in onion plant, allium chine ...	2013	23108875
rapid and sensitive ultrasonic-assisted derivatisation microextraction (udme) technique for bitter taste-free amino acids (faa) study by hplc-fld.	amino acids, as the main contributors to taste, are usually found in relatively high levels in bitter foods. in this work, we focused on seeking a rapid, sensitive and simple method to determine faa for large batches of micro-samples and to explore the relationship between faa and bitterness. overall condition optimisation indicated that the new udme technique offered higher derivatisation yields and extraction efficiencies than traditional methods. only 35min was needed in the whole operation p ...	2014	24054218
anticancer activity of saponins from allium chinense against the b16 melanoma and 4t1 breast carcinoma cell.	the cytotoxic substance of a. chinense saponins (acss) was isolated using ethanol extraction and purified with the d101 macroporous adsorption resin approach. we investigated the anticancer activity of acss in the b16 melanoma and 4t1 breast carcinoma cell lines. methylthioninium chloride and hematoxylin-eosin staining with giemsa dyestuff were used when the cells were treated with acss. the results showed that the cells morphologies changed significantly; acss induced cell death in b16 and 4t1 ...	2015	26146506
[research on volatiles of rakkyo (allium chinense g. don) and chinese chive (allium tuberosum rottl. ex sprengel) based on headspace and the molecular recognition of sers].	the head space and the molecular recognition of surface enhanced raman scattering (sers) were used to research volatiles of rakkyo and chinese chive. their volatiles sers spectra were obtained using nano-silver colloid as the substrate. then, volatiles sers spectra of rakkyo and chinese chive were compared respectively with the volatiles sers spectra of liquid allyl methyl sulfide, 1-propanethiol, diallyl disulfide and all possible pairings of the three compounds. the results showed that the rep ...	2015	25970899
purification and cloning of lectin that induce cell apoptosis from allium chinense.	a 8.7 kda lectin with high agglutin activity was isolated by affinity chromatography and cloned from allium chinense in this study. for the mtt assay, approximately 60 µg/ml a. chinense lectin (acl) inhibited 50% of the human hepatoma hep-3b cells grown after 48 h. in addition, no antiproliferative effect was observed on normal human umbilical vein endothelial cells (huvec) even at 100 µg/ml concentration. after treatments with acl on hep-3b cells, morphologic changes in the nucleus and cytoskel ...	2015	25765828
fermentation of allium chinense bulbs with lactobacillus plantarum zdy 2013 shows enhanced biofunctionalities, and nutritional and chemical properties.	in this study, fermentation of allium chinense bulbs was carried out with lactobacillus plantarum zdy 2013. a decrease in ph from 6.8 to 3.5 and a stable lactic acid bacteria population were observed during 7-d fermentation. the total phenolic content increased by 2.7-fold in the aqueous and ethanol extracts of a. chinense bulbs after fermentation. antioxidant capacity including 2,2-diphenyl-1-picrylhydrazyl radical-scavenging effect and reducing power of both extracts was significantly (p < 0.0 ...	2015	26308368
phytochemistry and pharmacology of allii macrostemonis bulbus, a traditional chinese medicine.	allii macrostemonis bulbus (xiebai in chinese), as a famous traditional chinese medicine, has great medicinal and dietary values since ancient times. in china, the dry bulbs of allium macrostemon and allium chinense are both used as its original plants. pharmacological studies have revealed that both of them could increase plasminogen activator activity and prolong the effect of coagulation to achieve antiplatelet aggregation which validates their traditional uses for the treatment of thoracic o ...	2016	27507199
quantification of inorganic arsenic exposure and cancer risk via consumption of vegetables in southern selected districts of pakistan.	human exposures to arsenic (as) through different pathways (dietary and non-dietary) are considered to be one of the primary worldwide environmental health risks to humans. this study was conducted to investigate the presence of as in soil and vegetable samples collected from agricultural lands located in selected southern districts of khyber pakhtunkhwa (kpk) province, pakistan. we examined the concentrations of total arsenic (tas), organic species of as such as monomethylarsonic acid (mma) and ...	2016	26820935