Publications

serological survey for the prevalence of antibodies to egg drop syndrome 1976 virus in domesticated and wild birds in israel.	a serological survey of chicken, turkey, goose and duck flocks for the presence of antibodies to egg drop syndrome virus 1976 (eds 76) has been carried out in israel. in most of the chicken flocks sampled egg production was not normal, but no antibodies against this virus were detected. likewise, turkey breeding flocks were similarly negative. all pekin duck flocks tested showed some serological activity. muscovy ducks that were reared in direct or indirect contact with pekin ducks had haemagglu ...	1980	18770279
mdbk nuclear factor-binding site of various serotypes of adenovirus dna.	a fraction with the ability to bind the terminal fragment of equine adenovirus (ead) dna was prepared from mdbk cell nuclei. the fraction (mdbk nuclear factor) bound to the terminal fragment of all human and animal adenovirus dnas examined except avian adenovirus eds-76. however, the terminal fragments of two animal adenoviruses, ead and bovine adenovirus type 3 (bad3), showed higher affinity for the nuclear factor than the others. the mdbk nuclear factor-binding site determined by footprinting ...	1986	3796315
comparative serological studies with egg drop syndrome virus.	the sensitivities of five serological tests (haemagglutination inhibition, elisa, serum neutralisation, fluorescent antibody and agar gel immunodiffusion) for detection of egg drop syndrome virus antibody were compared. in experimentally inoculated birds seroconversion was first detected at five days post-inoculation using haemagglutination inhibition, elisa, serum neutralisation and fluorescent antibody test, and at seven days post-inoculation using agar gel immunodiffusion. sera from birds exp ...	1986	18766569
differentiation of egg drop syndrome virus isolates by restriction endonuclease analysis of virus dna.	thirteen isolates of egg drop syndrome (eds) virus were compared by restriction endonuclease analysis of the virus dna. one virus, an australian chicken isolate, was distinguished from the others using the endonucleases ecorl, bamul, kpnl, hindlll, pstl and pvull, all of which recognise six base pair dna sequences. polyacrylamide gel restriction fragment patterns generated by haelll, hhall and taqi, which recognise four base pairs, allowed further differentiation of the virus isolates. nine chic ...	1988	18766751
in situ hybridization: an optimised detection protocol for a biotinylated dna probe renders it more sensitive than a comparable 35s-labelled probe.	the development of a highly sensitive biotinylated in situ hybridization protocol using egg drop syndrome virus infection of domestic fowl as a model is described. this protocol incorporates the use of a monoclonal antibody to biotin as the initial step in a peroxidase-based detection system. the detection of viral nucleic acid in formalin-fixed, paraffin-embedded sections using biotinylated and 35s-labelled probes is compared to the detection of viral antigen by immunocytochemistry. biotinylate ...	1989	2547814
identification and characterization of penton base and pviii protein of egg drop syndrome virus.	the nucleotide sequence and location of the penton base and pviii genes of the egg drop syndrome (eds) virus an avian adenovirus, were determined. the penton base gene is located at 34.8-38.8 map units. the coding sequence has a length of 1359 nt and encodes a polypeptide of 452 amino acids (aa) with a molecular weight of 51 105 da. the amino acid sequence shows a homology of 57.3 and 55.3% to the structural protein ih of human adenovirus serotype 2 (had2) and fowl adenovirus serotype 1 (fav1). ...	1997	9037737
close phylogenetic relationship between egg drop syndrome virus, bovine adenovirus serotype 7, and ovine adenovirus strain 287.	a cloned egg drop syndrome (eds) virus genomic dna fragment containing the protease gene has been identified and the complete nucleotide sequence of the protease and partial nucleotide sequence of the hexon genes has been determined. phylogenetic analysis of the protease gene has revealed eds virus to be genetically more closely related to bovine adenovirus type 7 (bav-7) and ovine adenovirus isolate 287 (oav287) than either of these two viruses are to other members of the genus mastadenovirus o ...	1997	9123875
the complete nucleotide sequence of the egg drop syndrome virus: an intermediate between mastadenoviruses and aviadenoviruses.	the complete nucleotide sequence of an avian adenovirus, the egg drop syndrome (eds) virus, was determined. the total genome length is 33,213 nucleotides, resulting in a molecular weight of 21.9 x 10(6). the gc content is only 42.5%. between map units 3.5 and 76.9, the distribution of open reading frames with homology to known genes is similar to that reported for other mammalian and avian adenoviruses. however, no homologies to adenovirus genes such as e1a, pix, pv, and e3 could be found. outsi ...	1997	9375018
hexon based pcrs combined with restriction enzyme analysis for rapid detection and differentiation of fowl adenoviruses and egg drop syndrome virus.	three different polymerase chain reactions (pcrs), two of them combined with restriction enzyme analysis (rea), were developed for detection and differentiation of all 12 fowl adenovirus (fav) serotypes and the egg drop syndrome (eds) virus. for primer construction fav1, fav10 and eds virus hexon proteins were aligned and conserved and variable regions were determined. two primer sets (h1/h2 and h3/h4) for single use were constructed which hybridize in three conserved regions of hexon genes. eac ...	1998	9766892
[analysis of core virion polypeptides from the pathogen causing chicken egg-drop syndrome].	the cores of egg-drop syndrome virus (eds-76) were isolated by the pyridine technique. eds-76 proved to be much more resistant to pyridine disruption than other adenoviruses and treatment with 10% pyridine did not lead to complete dissociation of capsid and cores; only increase of pyridine concentration to 20% produced satisfactory results. at least three polypeptides (24, 10.5, and 6.5 kda) were found in the core by sds-page, whereas the 40 kda reacting with the core is most probably not a core ...	1998	9864829
[structural and functional organization of avian adenovirus eds-76 genome. sequencing and analysis of the 0-10.6% fragment of viral dna].		1998	9720064
[cloning and sequence analysis of 55 k protein of egg drop syndrome virus].	for understanding the characteristics of genomic structure of egg drop syndrome virus(edsv).	1999	12569783
the complete genomic sequence of egg drop syndrome virus strain aav-2.	in the search for the genome of egg drop syndrome virus (edsv-76) chinese strain aav-2, part of restriction endonuclease physical map is analyzed, the complete genomic library is organized. on basis of this, the complete genome nucleotide sequences (32 838 bp in length, including terminal structures) are determined. the data analysis shows: compared with the other adenoviruses, strain aav-2 has more disparity on genomic structure and the distribution of open reading frame (orf). there are no cle ...	1999	18726483
detection and differentiation of avian adenoviruses: a review.	avian adenoviruses are a very diverse group of pathogens causing a variety of problems for poultry production. for a long time, the diagnosis of an adenovirus infection was restricted to the isolation of the respective virus followed by various serological typing methods, such as immunofluorescence assay, neutralization test or haemagglutination-inhibition test. in addition, restriction enzyme analysis has been reported for differentiation of avian adenoviruses. besides summarizing the classical ...	2000	19184805
the role of egg drop syndrome virus in acute respiratory disease of goslings.	an outbreak of severe acute respiratory disease characterized by tracheitis and bronchitis was observed in young goslings on a large-scale goose farm in hungary. histological examination revealed amphophilic intranuclear inclusion bodies in the superficial epithelial cells of the trachea and bronchi. adenovirus-like particles were detected by electron microscopy, and the virus isolated from the trachea and the lungs was identified as egg drop syndrome (eds) virus by serological and genomic exami ...	2001	19184901
innovation and discovery: the application of nucleic acid-based technology to avian virus detection and characterization.	polymerase chain reaction (pcr)-based approaches to the detection, differentiation and characterization of avian pathogens continue to be developed and refined. the pcrs, or reverse transcriptase-pcrs, may be general, designed to detect all or most variants of a pathogen, or to be serotype, genotype or pathotype specific. progress is being made with respect to making nucleic acid approaches more suitable for use in diagnostic laboratories. robotic workstations are now available for extraction of ...	2001	19184952
an in vitro and in vivo evaluation of the virulence of egg drop syndrome virus for the chicken reproductive tract.	the virulence of strains of egg drop syndrome (eds) 1976 virus for the female reproductive tract of chickens was assessed in vitro using oviduct organ cultures (ooc) prepared from precociously induced oviducts in young chicks by oestrogen treatment. ciliostasis, haemagglutination and virus titres in infected ooc supernatants, histology and immunoperoxidase test results indicated the pathogenic ability of the four viruses for the precocious oviducts. one of the isolates, eds tn4, produced higher ...	2001	19184868
genetic organization and sequence analysis of pviii, fiber and early region 4 of bovine adenovirus type 7.	the dna sequence of 8,810 nucleotides at the right end of bovine adenovirus type 7 (bav7) genome was determined and compared with similar regions of other adenoviruses. this genomic region of bav7 consists of sequences encoding partial 33k, pviii, fiber, putative early region 4 (e4) proteins and other unassigned proteins. however, bav7 e3 region is not present in the expected location between pviii and fiber as bav7 intergenic region between pviii and fiber genes is only 183 nucleotides. the pre ...	2002	12206309
odocoileus hemionus deer adenovirus is related to the members of atadenovirus genus.	the odocoileus hemionus deer adenovirus (odadv-1) causes systemic and local vasculitis and proves extremely lethal for mule deer. to characterize the virus, part of the genome flanking the fiber gene was cloned and sequenced. the sequence revealed two open-reading frames that mapped to pviii hexon-associated protein precursor and fiber protein of several other adenoviruses. the highest amino acid homology for pviii and fiber was found with the members of the proposed atadenovirus genus: ovine ad ...	2002	12038693
detection of egg drop syndrome virus antigen or genome by enzyme-linked immunosorbent assay or polymerase chain reaction.	mouse monoclonal antibodies (mabs) were produced against an indian isolate of egg drop syndrome (eds) virus and characterized. four hybridoma clones were secreting mabs that bound to a 100 kda protein, presumably the hexon protein. these mabs were found to cross-react with two other indian isolates of eds virus and to the reference uk 127 strain. three of these mabs were mapped to the same epitope compared with the other mab (f8), which bound to a different epitope. an antigen-capture enzyme-lin ...	2003	14522711
comparison of hemagglutination inhibition test and elisa in quantification of antibodies to egg drop syndrome virus.	a single-serum dilution elisa for egg drop syndrome (eds) virus-specific antibodies was developed. in testing 425 chicken sera it was found to have a 93.6% sensitivity and 98.7% specificity relative to a hemagglutination inhibition (hi) test. the correlation coefficient for elisa and hi titers was 0.793. the elisa was efficacious in quantification of both vaccinal and infection antibodies and could routinely be used for screening large numbers of field sera.	2004	15595213
phylogenetic analysis of the hexon loop 1 region of an adenovirus from psittacine birds supports the existence of a new psittacine adenovirus (psadv).	adenovirus infections in psittacine birds have been well known. most of these infections were caused by fowl adenoviruses (fadv). in this study, liver samples showing typical histological signs of an adenovirus infection were collected from poicephalus spp. with acute disease. a pcr amplifying the variable loop 1 region of the hexon gene was developed using primers located in two conserved pedestal regions. a pcr product of approximately 590 bp in size was amplified and sequenced. the sequence o ...	2005	15968473
development of a one-step strip test for the diagnosis of chicken infectious bursal disease.	a rapid diagnostic strip for chicken infectious bursal disease (ibd) was developed based on membrane chromatography using high-affinity monoclonal antibodies directed to chicken infectious bursal disease virus (ibdv). the diagnostic strip has high specificity for detection of chicken ibdv antigen and recognizes a variety of the virus isolates, including virulent and attenuated strains, with no cross-reactivity to other viruses, such as newcastle disease virus, marek's disease virus, infectious b ...	2005	16094819
detection of antibodies to egg drop syndrome virus in chicken serum using a field-based immunofiltration (flow-through) test.	a simple, user-friendly, and rapid method to detect the presence of antibodies to egg drop syndrome 76 (eds) virus in chicken sera based on an immunofiltration (flow-through) test was developed. purified eds virus antigen was coated onto nitrocellulose membranes housed in a plastic module with layers of absorbent filter pads underneath. following addition of serum to be tested and washing, monoclonal antibodies or polyclonal serum to chicken immunoglobulin g (igg) was used as a bridge antibody t ...	2007	17992943
effect of selection for phagocytosis in dwarf chickens on immune and reproductive characters.	in current study, phagocytosis product (pp) of peripheral blood monocytes was detected among 920 dwarf chickens (460 per sex) at 20 wk of age, and based on discrepancies of pp, the flock was grouped (the highest group, the medium group, and the lowest group). then serum hemagglutination inhibition antibody titers and subpopulations of t-lymphocytes of each group were examined after inoculations of avian influenza virus h5n2 inactivated vaccine (20 wk of age), avian influenza virus h9 inactivated ...	2008	18079448
isolation and identification of duck adenovirus 1 in ducklings with proliferative tracheitis in ontario.	increased mortality was reported in two flocks of muscovy ducklings from two consecutive hatches originating from the same breeder flock. coughing, dyspnea, and gasping were observed in some ducklings between 6 and 11 days of age. opaque white plugs of exudate were seen in the tracheas with some ducklings having multiple tracheal plugs. tracheal and bronchial epithelium was hyperplastic and superficial epithelial cells contained eosinophilic intranuclear viral inclusions. virus particles compati ...	2009	19630243
testing for viral contaminants of veterinary vaccines in hungary.	the safety of veterinary vaccines is of paramount importance and it is significantly jeopardised by extraneous agents such as bacteria, mycoplasma, chlamydia and viruses. several critical steps of vaccine manufacture involve a potential risk of viral contamination. viruses, as extraneous, agents can be divided into two main groups. group 1 agents, such as pestivirus, chicken anaemia virus (cav), and egg drop syndrome virus (edsv) are well-known to manufacturers and authorities. compendial detect ...	2010	20338783
development, standardization and assessment of pcr systems for purity testing of avian viral vaccines.	the european pharmacopoeia (ph. eur.) requires avian viral vaccines to be free of adventitious agents. purity testing is an essential quality requirement of immunological veterinary medicinal products (ivmps) and testing for extraneous agents includes monitoring for many different viruses. conventional virus detection methods include serology or virus culture, however, molecular tests have become a valid alternative testing method. nucleic acid testing (nat) is fast, highly sensitive and has a h ...	2010	20338785
genomic and antigenic characterization of the newly emerging chinese duck egg-drop syndrome flavivirus: genomic comparison with tembusu and sitiawan viruses.	duck egg-drop syndrome virus (dedsv) is a newly emerging pathogenic flavivirus causing avian diseases in china. the infection occurs in laying ducks characterized by a severe drop in egg production with a fatality rate of 5-15 %. the virus was found to be most closely related to tembusu virus (tmuv), an isolate from mosquitoes in south-east asia. here, we have sequenced and characterized the full-length genomes of seven dedsv strains, including the 5'- and 3'-non-coding regions (ncrs). we also r ...	2012	22764316
antiviral activity of aloe hijazensis against some haemagglutinating viruses infection and its phytoconstituents.	evaluation of the antiviral activities of flowers, flower-peduncles, leaves, and roots of aloe hijazensis against haemagglutinating viruses of avian paramyxovirus type-1 (apmv-1), avian influenza virus type a (ai-h5n1), newcastle disease virus (ndv), and egg-drop syndrome virus (edsv) in specific pathogen free (spf) chicken embryos were carried out. extract of the flowers and leaves showed relatively higher activity than the extracts of other plant parts. thirteen compounds were isolated from bo ...	2012	22941477
duck egg drop syndrome virus: an emerging tembusu-related flavivirus in china.	duck egg drop syndrome virus (dedsv) is a newly emerging pathogenic flavivirus isolated from ducks in china. dedsv infection mainly results in severe egg drop syndrome in domestic poultry, which leads to huge economic losses. thus, the discovery of ways and means to combat dedsv is urgent. since 2010, a remarkable amount of progress concerning dedsv research has been achieved. here, we review current knowledge on the epidemiology, symptomatology, and pathology of dedsv. a detailed dissection of ...	2013	23917842
respiratory disease due to current egg drop syndrome virus in pekin ducks.	severe acute respiratory symptoms with coughing, dyspnea, and gasping were reported in two flocks of 9-day-old pekin ducklings from different provinces. gross lesions, white exudate and mucous membrane congestion in the trachea as well as blue to purple color changes and sclerosis in lungs were observed. histological lesions revealed that the trachea and bronchial epithelium were hyperplastic and infiltrated by neutrophil granulocytes. egg drop syndrome virus (edsv) was differentially diagnosed ...	2013	23639475
epidemiology of egg drop syndrome virus in ducks from south korea.	egg drop syndrome virus (edsv) is an important pathogen of poultry that decreases egg production in chickens and causes respiratory disease in goslings. in 2011, we obtained serum samples from 139 domestic pekin ducks, 416 one-day-old pekin ducklings, and 75 wild ducks (67 mallards and 8 pintails) to survey their exposure to edsv. a total of 123 of 139 sera (88.5%) from pekin ducks, 396 of the ducklings (95.2%), and 16 of 67 mallards (23.9%) were positive. field cases of edsv in wild and domesti ...	2013	23776265
full genome sequence of egg drop syndrome virus strain fj12025 isolated from muscovy duckling.	egg drop syndrome virus (edsv) strain fj12025 was isolated from a 9-day-old muscovy duckling. the results of the sequence showed that the genome of strain fj12025 is 33,213 bp in length, with a g+c content of 43.03%. when comparing the genome sequence of strain fj12025 to that of laying duck original strain av-127, we found 50 single-nucleotide polymorphisms (snps) between the two viral genome sequences. a genomic sequence comparison of fj12025 and av-127 will help to understand the phenotypic d ...	2013	23969050
egg drop syndrome virus enters duck embryonic fibroblast cells via clathrin-mediated endocytosis.	previous studies of egg drop syndrome virus (edsv) is restricted to serological surveys, disease diagnostics, and complete viral genome analysis. consequently, the infection characteristics and entry routes of edsv are poorly understood. therefore, we aimed to explore the entry pathway of edsv into duck embryonic fibroblast (def) cells as well as the infection characteristics and proliferation of edsv in primary def and primary chicken embryo liver (cel) cells. transmission electron microscopy r ...	2015	26200954
gexp analyzer-based multiplex reverse-transcription pcr assay for the simultaneous detection and differentiation of eleven duck viruses.	duck viral pathogens primarily include the avian influenza virus (aiv) subtypes h5, h7, and h9; duck hepatitis virus (dhv); duck tembusu virus (dtmuv); egg drop syndrome virus (edsv); duck enteritis virus (dev); newcastle disease virus (ndv); duck circovirus (ducv); muscovy duck reovirus (mdrv); and muscovy duck parvovirus (mdpv). these pathogens cause great economic losses to china's duck breeding industry.	2015	26518004
application of cross-priming amplification (cpa) for detection of fowl adenovirus (fadv) strains.	fowl adenoviruses (fadvs) are widely distributed among chickens. detection of fadvs is mainly accomplished by virus isolation, serological assays, various polymerase chain reaction (pcr) assays, and loop-mediated isothermal amplification (lamp). to increase the diagnostic capacity of currently applied techniques, cross-priming amplification (cpa) for the detection of the fadv hexon gene was developed. the single cpa assay was optimised to detect all serotypes 1-8a-8b-11 representing the species ...	2015	25655263
antigen-sparing and enhanced efficacy of multivalent vaccines adjuvanted with immunopotentiators in chickens.	we previously described that immunopotentiators, cvcva5, increased the efficacy of h5 and h9 subtype avian influenza vaccines in chickens, ducks, and geese. in this study, we further investigated the effects of the cvcva5 for improving the efficacy of other univalent or multivalent inactivated vaccines. the immune response administrated with half-dose of monovalent vaccine plus cvcva5 were higher than those of one dose of monovalent vaccine without immunopotentiators as measured by levels of ant ...	2017	28603519
decreased egg production in laying hens associated with infection with genotype 3 avian hepatitis e virus strain from china.	to determine the relationship between decreased egg production and avian hev infection, thirty healthy 23-week-old hy-line variety brown layer hens were randomly divided into 3 groups with 10 hens per group. next, a genotype 3 avian hev strain from china was used to inoculate laying hens via oronasal or intravenous routes using a 50% chicken infectious dose of 500. all hens were necropsied at 14 weeks postinoculation (wpi). fecal virus shedding, viremia, seroconversion, serum alanine aminotransf ...	2017	28619140
tropism and infectivity of duck-derived egg drop syndrome virus in chickens.	egg drop syndrome virus (edsv) can markedly decrease egg production in laying hens. duck is the natural host of edsv. edsv derived from ducks abrogate egg drop in laying hens. we have previously confirmed that duck-derived edsvs have a variety of replication activities in chick embryo liver (cel) cells. however, it is currently unclear whether duck-derived edsv could display tropism and adaptation in laying hens. this study assessed whether duck-derived edsv can adapt to laying hens, and estimat ...	2017	28481913
development of a pcr assay for detection and differentiation of muscovy duck and goose parvoviruses based on ns gene characterization.	muscovy duck parvovirus (mdpv) and goose parvovirus (gpv) have both been found to cause high mortality and morbidity in muscovy ducklings. specific detection is often rife with false positives due to high identity at the genomic nucleotide level and antigenic similarity between mdpvs and gpvs. in this study, significantly variable regions were found, via non-structural (ns) comparison, between mdpv and gpv ns genes; however, ns genes were conserved within the mdpv and gpv groups. a polymerase ch ...	2018	30298830
unravelling the receptor binding property of egg drop syndrome virus (edsv) from the crystal structure of edsv fiber head.	egg drop syndrome virus (edsv) is an avian adenovirus that causes markedly decrease in egg production, and in the quality of the eggs when it infects chickens. until now, edsv virus-cell interactions are poorly understood, and the cellular receptor is still unknown. in the present study, we determined the atomic structure of the fiber head of edsv (residues 377-644) at 2.74 å resolution. structure comparison with the (chick embryo lethal orphan) celo long fiber head and human adenovirus fiber he ...	2019	31381914
development of novel subunit vaccine based on truncated fiber protein of egg drop syndrome virus and its immunogenicity in chickens.	egg-drop syndrome virus (edsv) is an avian adenovirus that causes markedly decrease in egg production and in the quality of the eggs when it infects chickens. in this report, we engineered truncated fiber protein containing the entire knob domain and part of the shaft region as a vaccine candidate. the protein was obtained in the soluble fraction in escherichia coli (e. coli), and expression level after nickel-affinity purification was 126 mg/l. by means of multiple characterization methods, it ...	2019	31442468
nitric oxide production and fc receptor-mediated phagocytosis as functional readouts of macrophage activity upon stimulation with inactivated poultry vaccines in vitro.	vaccine batches must pass routine quality control to confirm that their ability to induce protection against disease is consistent with batches of proven efficacy from development studies. for poultry vaccines, these tests are often performed in laboratory chickens by vaccination-challenge trials or serological assays. the aim of this study was to investigate innate immune responses against inactivated poultry vaccines and identify candidate immune parameters for in vitro quality tests as altern ...	2020	32580391