Publications

identity of the pink-pigmented methanol-oxidizing bacteria as vibrio extorquens.	stocks, peter k. (louisiana state university, baton rouge), and c. s. mccleskey. identity of the pink-pigmented methanol-oxidizing bacteria as vibrio extorquens. j. bacteriol. 88:1065-1070. 1964.-pink-pigmented bacteria isolated from enrichment cultures of methane oxidizers were found to possess similar morphological, cultural, and physiological characteristics. all the strains utilized methanol, formate, oxalate, succinate, glycerol, and benzene as sole carbon sources; methanol, formate, and gl ...	1964	14219020
microbial growth on c1 compounds. the role of folat in the metabolism of pseudomonas am1.	1. the growth of pseudomonas am1 is much more sensitive to inhibition by sulphanilamide when methanol, rather than succinate, acts as the sole carbon and energy source; a sulphanilamide concentration of 1mm, which causes almost complete inhibition of growth on methanol, has little effect in a succinate medium. 2. similar results have been obtained with sulphadiazine and sulphathiazole. sulphanilic acid has little effect. 3. a similar differential sensitivity to sulphanilamide is shown by protami ...	1966	4957628
microbial growth on oxalate by a route not involving glyoxylate carboligase.	1. the metabolism of oxalate by the pink-pigmented organisms, pseudomonas am1, pseudomonas am2, protaminobacter ruber and pseudomonas extorquens has been compared with that of the non-pigmented pseudomonas oxalaticus. 2. during growth on oxalate, all the organisms contain oxalyl-coa decarboxylase, formate dehydrogenase and oxalyl-coa reductase. this is consistent with oxidation of oxalate to carbon dioxide taking place via oxalyl-coa, formyl-coa and formate as intermediates, and also reduction o ...	1970	5472155
methylmalonyl-coa mutase in a methanol-utilizing bacterium. protaminobacter ruber.		1976	12008
form of vitamin b(12) and its role in a methanol-utilizing bacterium protaminobacter ruber.	a methanol-utilizing bacterium, protaminobacter ruber, produced a large amount of vitamin b(12). the compounds were isolated from the cells and identified as methylcobalamin (methyl-b(12)) and adenosylcobalamin (adenosyl-b(12)) by various tests. the variation in the form of b(12) during cultivation was examined by bioautography with cellulose acetate membrane electrophoresis. methyl-b(12) and adenosyl-b(12) were the two main b(12) compounds produced in the various phases of bacterial growth. the ...	1977	16345200
role of vitamin b12 and enzymes related to methylmalonyl-coa mutase in a methanol-utilizing bacterium, protaminobacter ruber.	a methanol-utilizing bacterium, protaminobacter ruber, required cobalt ion or vitamin b12 as its growth factor, which could be replaced by succinate among various additions to the cobalt-deficient medium. the presence of adenosylcobalamin (adenosyl-b12)-dependent methylmalonyl-coenzyme a (coa) mutase was demonstrated in the cell-free extracts of p. ruber. the specific activity of this mutase was not only fairly high in comparison with that reported with other organisms but also detected at a sim ...	1978	32234
bacteriochlorophyll formation by facultative methylotrophs, protaminobacter ruber and pseudomonas am 1.		1978	620799
isoprenoid quinones in the classification of coryneform and related bacteria.	menaquinones were the only isoprenoid quinones found in 85 of the 95 coryneform bacteria examined. dihydromenaquinones having nine isoprene units were the main components isolated from corynebacterium bovis, from other glutamic acid-producing strains, and from arthrobacter globiformis and related species. dihydromenaquinones with eight isoprene units were found in brevibacterium linens, the remaining corynebacterium species and strains probably belonging to the genus rhodococcus. tetrahydromenaq ...	1979	107269
lipoquinones of some spore-forming rods, lactic-acid bacteria and actinomycetes.	the respiratory quinones of 73 strains of gram-positive bacteria including spore-forming rods, lactic-acid bacteria and actinomyctes were examined. menaquinones with seven isoprenoid units (mk-7) were the main quinone type found in representatives of the genus bacillus and in sporolactobacillus inulinus. however, a strain of b. thuringiensis produced mk-8 in addition to mk-7, and strains of b. lentus and b. pantothenticus appeared to produce mk-9 and mk-8, respectively, with no mk-7. in the clos ...	1979	119033
level of methionine synthase activity and interconversion of methylcobalamin and adenosylcobalamin in a facultative methylotroph, protaminobacter ruber.	protaminobacter ruber was cultured in a medium containing [57co]cyanocobalamin with a "two-step cultivation method" and the forms of vitamin b12 compounds in the cells were examined. methylcobalamin was detected in the early phases of growth and reached a maximum of about 40% of all cobalamins extracted from the cells. in the stationary phase of growth, almost all cobalamins consisted of adenosylcobalamin. recultivation of the cells of the stationary phase in a fresh medium resulted in the conve ...	1980	7241237
regulation of vitamin b12 and bacteriochlorophyll biosynthesis in a facultative methylotroph, protaminobacter ruber.	the regulation of vitamin b12 and bacteriochlorophyll formation was studied in a facultative methylotroph, protaminobacter ruber, classified as a non-photosynthetic bacterium. vitamin b12 was formed at an almost constant level under various cultivation conditions, while bacteriochlorophyll synthesis was drastically influenced by the growth conditions. addition of b12 and hemin to the medium and the change of culture conditions from light to darkness in the early growth phases stimulated the pigm ...	1981	7320769
the presence of glutamate mutase in a methanol-utilizing bacterium, protaminobacter ruber.	cell-free extracts of a facultative methylotroph and strict aerobe, protaminobacter ruber, could catalyze formation of beta-methylaspartate from glutamate. beta-methylaspartate formed was further converted to mesaconate. from these results, it was found that the cells of p. ruber contained a sequential reaction system of glutamate mutase and beta-methylaspartase. the level of glutamate mutase activity was almost constant throughout the period of cultivation. when p. ruber was grown on several no ...	1982	7097372
assay, purification and characterization of cob(i)alamin adenosyltransferase of protaminobacter ruber.	a simple spectrophotometric method for assay of atp: cob(i)alamin co-beta-adenosyltransferase was established. by using this method, specific activity of the enzyme in protaminobacter ruber was found to be the highest in the logarithmic growth phase. the enzyme was highly purified from the cells in that phase and characterized. the molecular weight of the enzyme was estimated as 44,000 by gel filtration. the optimal ph and temperature were about 8.0 and 50 degrees c, respectively. among divalent ...	1984	6441836
pseudomonas mesophilica and an unnamed taxon, clinical isolates of pink-pigmented oxidative bacteria.	twenty-one strains of pink-pigmented bacteria, isolated from human clinical specimens and an environmental source, were compared with pseudomonas mesophilica atcc 29983 and protaminobacter ruber atcc 8457. these isolates were gram-negative, oxidative rods which were motile by means of a single polar flagellum; gave positive catalase, indophenol oxidase, urease, and amylase reactions; and grew slowly at 30 degrees c. fourteen isolates conformed to the designated type strains pseudomonas mesophili ...	1984	6490848
safety evaluation of protaminobacter rubrum: intravenous pathogenicity and toxigenicity study in rabbits and mice.	the iv pathogenicity and toxigenicity of protaminobacter rubrum was studied in new zealand white rabbits and cf1 br mice. following a probe study, nine groups of six rabbits each were injected iv with; 1 ml of viable-cell suspension (vcs) at concentrations of 2.23 x 10(8), 10(6), 10(4) and 10(2) organisms/ml; the cell-free supernatant (cfs; in which the test organism had been cultured to a concentration of approximately 3 x 10(10) cells/ml) at dilutions of 1:100, 1:10,000 and 1:1,000,000 in phos ...	1991	1959822
synthesis of 1',6'-disubstituted sucroses and their behavior as glucosyl donors for a microbial alpha-glucosyltransferase.	versatile 6'-chloro-6'-deoxy-1'-substituted sucrose derivatives were synthesized in search of an optimum donor substrate for the intermolecular transglucosylation with the alpha-glucosyltransferase from protaminobacter rubrum. two substituents at the c-1' and c-6' positions of sucrose were introduced utilizing the distinct reactivity of the corresponding sulfonates. methyl beta-d-arabinofuranoside was most efficiently glucosylated with the 1'-deoxy derivative 5. hydroxyl and fluoro groups at c-1 ...	1996	8625358
proposition for the biochemical mechanism occurring in the sucrose isomerase active site.	sucrose conversion by sucrose isomerase from protaminobacter rubrum, serratia plymuthica and erwinia rhapontici was investigated in the presence of different monosaccharides in the reaction mixture. these conditions led to inhibitory effects and to glucosyl transfer of the glucose moiety of sucrose to the exogen monosaccharide. comparison of the structure of the different inhibitors and acceptors has allowed us to suppose the binding of the sucrose molecule into the active site, and thereafter, ...	1998	9891968
characterization of pantoea dispersa uq68j: producer of a highly efficient sucrose isomerase for isomaltulose biosynthesis.	isolation, identification and characterization of a highly efficient isomaltulose producer.	2004	15186446
[conversion of androstenedione and androstadienedione by sterol-degrading bacteria].	the composition of steroid metabolites formed during the conversion of androstenedione and androstadienedione, products of degradation of sterol side chains by soil and mutant strains of the bacterial genera mycobacterium and protaminobacter, was studied. testololactone was absent from the conversion products. this favors the idea of different cleavage pathways of steroid ring d in bacteria and fungi. very small amounts of two new 14alpha-hydroxy derivatives with cleaved b ring were isolated aft ...	2004	15553785
overexpression, purification, crystallization and preliminary diffraction studies of the protaminobacter rubrum sucrose isomerase smua.	palatinose (isomaltulose, alpha-d-glucosylpyranosyl-1,6-d-fructofuranose), a nutritional and acariogenic reducing sugar, is industrially obtained from sucrose by using immobilized cells of protaminobacter rubrum that produce the sucrose isomerase smua. the isomerization of sucrose catalyzed by this enzyme also results in the formation of trehalulose (alpha-d-glucosylpyranosyl-1,1-d-fructofuranose) in smaller amounts and glucose, fructose and eventually isomaltose as by-products, which lower the ...	2006	16511267
isomaltose production by modification of the fructose-binding site on the basis of the predicted structure of sucrose isomerase from "protaminobacter rubrum".	"protaminobacter rubrum" sucrose isomerase (si) catalyzes the isomerization of sucrose to isomaltulose and trehalulose. si catalyzes the hydrolysis of the glycosidic bond with retention of the anomeric configuration via a mechanism that involves a covalent glycosyl enzyme intermediate. it possesses a (325)rldrd(329) motif, which is highly conserved and plays an important role in fructose binding. the predicted three-dimensional active-site structure of si was superimposed on and compared with th ...	2008	18552181
isomaltulose production from sucrose by protaminobacter rubrum immobilized in calcium alginate.	different culture conditions for protaminobacter rubrum and enzymatic reaction parameters were evaluated with the goal of improving isomaltulose production. p. rubrum was grown in a medium with 1% (w/v) cane molasses and 0.5% yeast extract and achieved a maximum cell yield y(x/s) of 0.295 g of cells/g sucrose and a specific growth rate (mu) of 0.192 h(-1). the immobilization of p. rubrum cells was carried out with calcium alginate, glutaraldehyde and polyethyleneimine. stabile immobilized cell p ...	2009	19410450
structural determinants of product specificity of sucrose isomerases.	the healthy sweetener isomaltulose is industrially produced from the conversion of sucrose by the sucrose isomerase smua from protaminobacter rubrum. crystal structures of smua in native and deoxynojirimycin complexed forms completed with modeling studies unravel the characteristics of the isomaltulose synthases catalytic pocket and their substrate binding mode. comparison with the trehalulose synthase mutb highlights the role of arg(298) and arg(306) active site residues and surface charges in ...	2009	19427862
microbial sucrose isomerases: producing organisms, genes and enzymes.	sucrose isomerase (si) activity is used industrially for the conversion of sucrose into isomers, particularly isomaltulose or trehalulose, which have properties advantageous over sucrose for some food uses. all of the known microbial sis are tim barrel proteins that convert sucrose without need for any cofactors, with varying kinetics and product specificities. the current analysis was undertaken to bridge key gaps between the information in patents and scientific publications about the microbes ...	2012	22133441
Mechanism-oriented redesign of an isomaltulose synthase to an isomelezitose synthase by site-directed mutagenesis.	An isomelezitose synthase was redesigned out of the sucrose isomerase from Protaminobacter rubrum for the synthesis of isomelezitose (6-O(F) -glucosylsucrose), a potential nutraceutical. The variants F297A, F297P, R333K, F321A_F319A and E428D catalyze the formation of isomelezitose in up to 70?% yield.	2012	22124943