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entomo-virological study of a suspected japanese encephalitis outbreak in muzaffarpur district, bihar, india. 023760390
litchi-associated acute encephalitis in children, northern vietnam, 2004-2009.since the end of the 1990s, unexplained outbreaks of acute encephalitis in children coinciding with litchi harvesting (may-july) have been documented in the bac giang province in northern vietnam. a retrospective ecologic analysis of data for 2004-2009 involving environmental, agronomic, and climatic factors was conducted to investigate the suspected association between the outbreaks and litchi harvesting. the clinical, biological, and immunologic characteristics of the patients suggested a vira ...023092599
entomological investigations into an epidemic of japanese encephalitis (je) in northern districts of west bengal, india (2011-2012).japanese encephalitis (je) is one of the most important arboviral diseases of human beings with outbreaks in many parts of southeast asia including india. we present the entomological findings of an outbreak occurred in northern part of west bengal during 2011-2012 with special emphasis on the role of je vectors in different seasons.025027086
bothaella manhi, a new species of tribe aedini (diptera: culicidae) from the cuc phuong national park of vietnam based on morphology and dna sequence.a new species of genus bothaella (diptera: culicidae) collected along with two other species of the genus during surveys for flavivirus isolations in the cuc phuong national park in northern vietnam is formally described and named as bothaella manhi, sp. n. the adults, pupa and fourth-instar larva are characterized, the male genitalia and the two immature stages are illustrated and dna sequence data are included for regions coding for sections of the coi and coii genes (mtdna). the species is co ...023155353
remarkable diversity of endogenous viruses in a crustacean genome.recent studies in paleovirology have uncovered myriads of endogenous viral elements (eves) integrated in the genome of their eukaryotic hosts. these fragments result from endogenization, that is, integration of the viral genome into the host germline genome followed by vertical inheritance. so far, most studies have used a virus-centered approach, whereby endogenous copies of a particular group of viruses were searched in all available sequenced genomes. here, we follow a host-centered approach ...025084787
mosquito hemocyte-mediated immune responses.hemocytes are a key component of the mosquito immune system that kill pathogens via phagocytic, lytic and melanization pathways. individual mosquitoes contain between 500 and 4,000 hemocytes, which are divided into three populations named granulocytes, oenocytoids and prohemocytes. hemocytes can also be divided by their anatomical location with 75% of hemocytes circulating in the hemocoel (circulating hemocytes) and 25% of hemocytes attaching themselves to tissues (sessile hemocytes). greater th ...025309850
molecular cloning and mrna expression of the peptidoglycan recognition protein gene hcpgrp1 and its isoform hcpgrp1a from the freshwater mussel hyriopsis cumingi.peptidoglycan recognition proteins (pgrps) are innate immune molecules that have been structurally conserved throughout evolution in invertebrates and vertebrates. in this study, peptidoglycan recognition protein hcpgrp1 and its isoform hcpgrp1a were identified in the freshwater mussel hyriopsis cumingii. the full-length cdnas of hcpgrp1 (973 bp) and hcpgrp1a (537 bp) encoded polypeptides with 218 and 151 amino acids, respectively. sequence analysis showed that hcpgrp1 had one c-terminal pgrp do ...025249773
mosquito larvicidal potential of four common medicinal plants of india.mosquitoes transmit serious human health diseases, causing millions of deaths every year. plants may be sources of alternative mosquito control agents. the present study was carried out to assess the role of larvicidal activities of the crude extracts of four plants viz. alternanthera sessilis l. (amaranthaceae), trema orientalis l. (cannabaceae), gardenia carinata smith. (rubiaceae) and ruellia tuberosa l. (acanthaceae) against culex quinquefasciatus say in laboratory bioassay.025222784
the aquatic communities inhabiting internodes of two sympatric bamboos in argentinean subtropical forest.in order to determine if phytotelmata in sympatric bamboos of the genus guadua might be colonized by different types of arthropods and contain communities of different complexities, the following objectives were formulated: (1) to analyze the structure and species richness of the aquatic macroinvertebrate communities, (2) to comparatively analyze co-occurrences; and (3) to identify the main predators. field studies were conducted in a subtropical forest in argentina, where 80 water-filled bamboo ...024224775
surveillance and vector control of lymphatic filariasis in the republic of korea.until the early 2000s, lymphatic filariasis would commonly break out in the coastal areas in korea. through steady efforts combining investigation and treatment, filariasis was officially declared eradicated in 2008. this study surveyed the density of vector species of filariasis in past endemic areas, and inspected filariasis dna from collected mosquitoes for protection against the reemergence of filariasis.024159506
filariasis of uncommon nature in india. 023509678
intron retention in the drosophila melanogaster rieske iron sulphur protein gene generated a new protein.genomes can encode a variety of proteins with unrelated architectures and activities. it is known that protein-coding genes of de novo origin have significantly contributed to this diversity. however, the molecular mechanisms and evolutionary processes behind these originations are still poorly understood. here we show that the last 102 codons of a novel gene, noble, assembled directly from non-coding dna following an intronic deletion that induced alternative intron retention at the drosophila ...021610726
vector mosquitoes of filariasis in japan. 022028600
brugian filariasis in japan. 022028598
basic studies on filaria and filariasis. 022028602
identification and molecular characterization of a prophenoloxidase involved in aedes aegypti chorion melanization.chorion melanization is a vital biochemical event for the survival of mosquito eggs in the environment. this study describes the identification and molecular characterization of a prophenoloxidase (propo) involved in chorion melanization in aedes aegypti by various biochemical and molecular techniques. results revealed that transcription of the chorion propo occurs only in adults, blood feeding greatly stimulated its transcription and haemocytes are responsible for its transcription. our study p ...015796752
effects of elevated atmospheric co(2) on water chemistry and mosquito (diptera: culicidae) growth under competitive conditions in container habitats.we investigated the direct and indirect effects of elevated atmospheric co(2) on freshwater container habitats and their larval mosquito occupants. we predicted that a doubling of atmospheric co(2) would (1) alter the chemical properties of water in this system, (2) slow degradation of leaf litter, and (3) decrease larval growth of aedes albopictus (skuse) mosquitoes raised on that litter under competitive conditions. effects of elevated co(2) on water quality parameters were not detected, but t ...022661767
description of the transcriptomes of immune response-activated hemocytes from the mosquito vectors aedes aegypti and armigeres subalbatus.mosquito-borne diseases, including dengue, malaria, and lymphatic filariasis, exact a devastating toll on global health and economics, killing or debilitating millions every year (54). mosquito innate immune responses are at the forefront of concerted research efforts aimed at defining potential target genes that could be manipulated to engineer pathogen resistance in vector populations. we aimed to describe the pivotal role that circulating blood cells (called hemocytes) play in immunity by gen ...015213157
the colonization and laboratory maintenance of armigeres subalbatus (coquillett). 014272435
notes on the colonization and biology of armigeres subalbatus (diptera, culicidae). 196414183103
a study of filaria in taiwan monkeys.filarial adult were found in the intermuscular connective tissue of the peritracheal and mandibular region of the taiwan monkey(macaca cyclopis). the microfilariae in the peripheral blood demonstrated a marked nocturnal periodicity with a peak between 2100-0100 hours and a low diurnal minimum between 900 and 1300 hours. staining the microfilaria by various stains demonstrated differences in size. no development of microfilariae was detected in the culex fatigans, culex tritaeniohyncus, aedes alb ...196412913612
observations on the survival rate of armigeres subalbatus after being fed on hosts with different levels of micro-filariae of brugia pahangi. 19684386489
double infection in a mosquito armigeres subalbatus (coquillett) (a. obturbans auct.) with two different species of filaria. 19704392055
relation between diel activity and respiration in armigeres subalbatus (coquillett) (diptera: culicidae). 1975498
infective larvae of brugia: escape from mosquitoes into water and subsequent oral infectivity in jirds.published work showed that third-stage larvae (l-3s) escape into water from dead or dying, brugia pahangi-infected, aedes aegypti. the present study revealed the same escape phenomenon among b. pahangi-infected armigeres subalbatus, anopheles quadrimaculatus, and aedes togoi, and among brugia malayi-infected ae. aegypti and ae. togoi. l-3s maintained in water or in lum's solution for 3 hours retained infectivity when tested in orally or subcutaneously exposed jirds; furthermore, l-3s recovered f ...19768999
rhythms in the biting behaviour of a mosquito armigeres subalbatus.the biting cycle of armigeres subalbatus is distinctly crepuscular, exhibiting two peaks of activity, a smaller one at dawn and a larger one at dusk. the biting cycle is entrained to natural light-dark cycles and the time interval from dawn to dawn or dusk to dusk peaks is exactly 24 h and from dawn to dusk or dusk to dawn is about 12 h measured at 50% level. this rhythm manifests itself day after day without any marked qualitative change.the rate of change of light intensity may determine the o ...198028309633
laboratory studies on the biology of armigeres subalbatus coquillett, 1898. 19816116668
armigeres subalbatus incriminated as an important vector of the dog heartworm dirofilaria immitis and the bird cardiofilaria in urban kuala lumpur. 19816124044
diurnal man-biting activity of armigeres subalbatus (coquillett, 1898) in a village in west bengal. 19836143725
[preliminary report on the susceptibility of mosquitoes to brugia pahangi].a comparative study on the susceptibility of eight species of four genera of mosquitoes to brugia pahangi was carried out. aedes aegypti (liverpool strain), aedes togoi, armigeres subalbatus and mansonia uniformis (taiwan strains) were found with the third-stage larvae 14 days after feeding on infected rats (sprague-dawley). the remaining of aedes aegypti, aedes albopictus, aedes pseudoalbopictus, culex p. fatigans and culex annutus (taiwan strains) were found refractory to infection. to determi ...19846150813
getah virus in several species of mosquitoes.vector competence of aedes (ae.) vexans nipponii (nip.) and culex (cx) tritaeniorhynchus for getah virus was compared after incubation at 28 degrees c and 20 degrees c. marked differences existed between ae. vexans nip. strains sapporo and cx tritaeniorhynchus strain kyoto in infection and transmission rates following ingestion of blood meals containing several concentrations of getah virus. simultaneous comparison of infectivity also revealed that infection rates of ae. vexans nip. strains sapp ...19854082267
seasonal prevalence of the vector mosquitoes of japanese encephalitis virus in kyungpook province, korea.in order to determine the seasonal prevalence and population density of vector mosquitoes in kyungpook province, a survey based on average number of female mosquito per trap-night, were carried out during the period from may to november in 1984. among the 34,571 mosquitoes collected in kyungsan county in animal shelters and human dwellings by light traps, approximately 45.0 % were culex tritaeniorhynchus, 34.0 % per cent culex pipiens pallens, and 19.0 per cent anopheles sinensis. by comparison, ...198512888695
effectiveness of soap formulations containing deet and permethrin as personal protection against outdoor mosquitoes in malaysia.two soap formulations, both containing 20% deet and one each containing permethrin at 0.5 and 1.0%, respectively, were applied to exposed arms and legs of volunteers as personal protection against outdoor human biting mosquitoes in six locations on penang island, malaysia. the predominant mosquito species collected from these locations were aedes albopictus, mansonia uniformis, culex gelidus, anopheles lesteri and armigeres subalbatus. efficacy and residual effects up to 4 hours indicated good p ...19862906963
inheritance of orange pupa and phosphoglucomutase in the mosquito armigeres subalbatus. 19872904972
evaluation of permethrin-impregnated mosquito-nets against mosquitoes in china.in yishan county, guangxi province, china, where malaria and brugian filariasis are transmitted by vectors belonging to the anopheles hyrcanus pallas group, a study was conducted from june to november 1986 to evaluate the effectiveness of permethrin-impregnated mosquito bednets to reduce man/vector contact. in three experimental villages a total of 246 mosquito nets were treated with permethrin ec at a dosage of 0.5 g/m2. control bioassays (1 h exposure) of treated nets gave up to 100% mortality ...19882908784
[the experimental pathogenic effect of spiroplasma isolated from mosquitoes on the hatching of aedes aegypti ova and the growth of larva hatched from these eggs].the authors did study the experimental effects on aedes aegypti ova of different spiroplasma strains, isolated from mosquitoes in french savoy and in taiwan. the sp7 strain, from armigeres subalbatus (taiwan), demonstrates a true pathogenic effect on the larval evolution, without sex ratio modifications, nor bacterial transmission to the adult mosquitoes. the authors present their results and emphasize the difficult use of spiroplasmas sp.19883400962
brugia malayi and brugia pahangi: inherent difference in immune activation in the mosquitoes armigeres subalbatus and aedes aegypti.the inherent ability of brugia malayi and brugia pahangi (nematoda) to establish successful relationships with the mosquitoes armigeres subalbatus and aedes aegypti liverpool strain was evaluated. brugia pahangi microfilariae (mff) avoided the immune response and developed normally in a. subalbatus exposed to the parasite by an infective bloodmeal, whereas nearly 85% of b. malayi were destroyed by the immune response. because a. aegypti supports the development of both filarial worm species but ...19892563767
experimental vertical transmission of japanese encephalitis virus by culex tritaeniorhynchus and other mosquitoes.vertical transmission of japanese encephalitis virus to the f1 adult stage was demonstrated in culex tritaeniorhynchus, cx. annulus, cx. quinquefasciatus, and armigeres subalbatus. transmission to the f1 larval stage was demonstrated in cx. pipiens, aedes vexans, ae. alcasidi, and a. flavus. in cx. tritaeniorhynchus, vertical transmission rates (the percentage of parent females transmitting to progeny) varied (12-100%). filial infection rates (the percentage of progeny infected) for a given mosq ...19892567124
breeding habitats of mosquitoes in goa.studies carried out in goa from 1986 to 1987 revealed immature stages of 43 species of mosquitoes. breeding habitats were divided into three categories, viz., (i) ground water habitats consisting of ground pools and tanks, rocky pools, paddy fields and stream beds, yielding 30 species; (ii) plant habitats consisting of tree holes and fallen coconut shells and leaf sheaths yielding 20 species; and (iii) domestic/peridomestic habitats consisting of cement tanks, glass, earthen, metallic containers ...19892572463
chromosomal localization and copy number of 18s + 28s ribosomal rna genes in evolutionarily diverse mosquitoes (diptera, culicidae).in situ hybridization using 3h-labeled 18s and 28s ribosomal dna (rdna) probes from aedes albopictus was performed on the mitotic chromosomes of 20 species of mosquitoes belonging to 8 genera of subfamilies culicinae and anophelinae. in all but one species examined, the rdna family was localized to a single chromosome per haploid genome. aedes triseriatus was the only exception, with the rdna cistrons present on chromosome 1 and on chromosome 3. the ribosomal rna genes were located on chromosome ...19902093704
evaluation of the control of mosquitoes with insect growth regulators.in this study, the effectiveness of eight insect growth regulators (igrs) (chlorfluazuron, diflubenzuron, el-494, flufenoxuron, teflubenzuron, juglone, plumbagin and methoprene) against five mosquito vectors (armigeres subalbatus, aedes albopictus, aedes aegypti, culex tritaeniorhynchus, and culex quinquefasciatus) was investigated in the laboratory. the ec50s of chlorfluazuron, diflubenzuron, el-494, flufenoxuron, teflubenzuron, and methoprene against the five mosquitoes ranged from 0.0001 to 0 ...19902402025
tralomethrin (oms-3048), a new synthetic pyrethroid against vector mosquitoes.tralomethrin (oms-3048) a synthetic pyrethroid, was tested for its insecticidal properties in the laboratory against normal strains of culex quinquefasciatus, cx. tritaeniorhynchus, cx. sitiens, anopheles stephensi, an. culicifacies, aedes aegypti and armigeres subalbatus and against strains of cx. quinquefasciatus resistant to fenthion and malathion. tralomethrin showed good larvicidal activity against all the species tested. lc50 ranged between 7.00 x 10(-6) and 9.10 x 10(-3) mg (ai)/l. resist ...19911688021
comparison of the effectiveness of two deet formulations against aedes albopictus in the philippines.the effectiveness of 2 formulations of deet was tested in the philippines against aedes albopictus. a new military issue extended duration repellent formulation (edrf) was compared with the older standard liquid formulation. consistently fewer mosquitoes landed on subjects treated with the edrf than on those treated with the liquid formulation for up to 12 h post-application. however, the difference between the 2 formulations was not statistically significant. both treatments were significantly ...19911686272
haemolymph monophenol oxidase activity in armigeres subalbatus infected with brugia pahangi.the activity of monophenol oxidase can be elicited in the haemolymph of armigeres subalbatus by both blood and filaria-infected blood feeding. haemolymph collected from both blood-fed and filaria-infected mosquitoes was investigated using a quantitative radiometric assay that measured the amount of tritiated water formed during the hydroxylation of l-[3,5-3h]tyrosine to dopa. enzyme activity in filaria-infected mosquitoes was found to be significantly lower than that found in the blood-fed mosqu ...19911680109
survey of peridomestic mosquito species of jaffna peninsula in sri lanka.surveys of mosquitos in sri lanka have generally been confined to the south. a recent study in jaffna peninsula in northern sri lanka reported on the biology of peridomestic mosquito species. the present survey indicates the predominant species to be armigeres durhami (edwaards) and armigeres subalbatus (coquillett). aedes albopictus (skuse), ae. aegypti (linnaeus) and ae. novalbopictus barraud were recorded only occasionally during the survey. sampling of ponds in the jaffna peninsula recorded ...19911687931
a study on the ecological habit of armigeres subalbatus in dawa area of the mengshan mountain in shandong province].armigeres subalbatus (a.s.) was reported in shandong province for the first time in 1965 and found in five counties of south shandong including pingyi, linyi etc. in 1986. in dawa area of the mengshan mountain a.s. alults could be found in the first ten days of may, which increased in number in july, and become the dominant species in mosquito colonies in aug. and sept., then decreased gradually in number in oct. and disappeared in nov. there were two peaks of activity and blood-sucking behavior ...19921356655
larval habitat of armigeres subalbatus (coq) and its characteristics in pondicherry.larval habitats of armigeres subalbatus were delineated in urban and rural areas of pondicherry. survey of various potential mosquito breeding habitats revealed that septic tanks were the typical breeding habitat in both areas and the proportion was significantly higher in urban areas. the productive status of septic tanks differed in different months and the overall proportion breeding ar. subalbatus was significantly higher in urban areas (0.0447) compared to rural areas (0.0181). sporadic bre ...19921362629
beta-cyfluthrin, a synthetic pyrethroid for mosquito control.beta-cyfluthrin (oms 3051), a new synthetic pyrethroid and one of the stereoisomers of cyfluthrin, was studied for insecticidal activity against eight mosquito species. its larvicidal activity with lc50 values of 5.62 x 10(-5) and 1.19 x 10(-4) mg/l respectively for culex quinquefasciatus and aedes aegypti was comparable with that of deltamethrin. this pyrethroid was more effective against the larvae of armigeres subalbatus (lc50 - 7.76 x 10(-7)) and the adults of anopheles culicifacies lt50 - 2 ...19921359650
cannibalistic behavior in armigeres subalbatus (diptera: culicidae).phenomenon of natural cannibalism was observed to be exhibited by late (iii and iv) instar larvae of armigeres subalbatus. cannibalistic behavior in this species was studied in response to food and density. cannibalism among late instars was found to occur even in the presence of an adequate quantity of food. the rate of cannibalism was enhanced when food was restricted to only the early stages. even in the total absence of food early instars did not show any cannibalistic behavior. density had ...19921362627
reproductive costs associated with resistance in a mosquito-filarial worm system.the mosquito armigeres subalbatus can encapsulate and kill > 80% of brugia malayi microfilariae (mf) within 36 hr following ingestion. the cascade of biochemical events constituting this melanotic encapsulation response is also important in other mosquito biological events, including egg-chorion tanning. certain biochemical entities, including a tyrosine precursor, are thought to be shared among these biological activities. because of this purported tyrosine link, and because the blood meal both ...19937904130
the suitability of restriction fragment length polymorphism markers for evaluating genetic diversity among and synteny between mosquito species.restriction fragment length polymorphism (rflp) markers derived from the yellow fever mosquito, aedes aegypti, were used in hybridizations to genomic dna of the following mosquito species: ae. albopictus, ae. togoi, armigeres subalbatus, culex pipiens, and anopheles gambiae. interspecific hybridization with ae. aegypti probes varied from 50% (an. gambiae) to 100% (ae. albopictus) under high stringency conditions. we demonstrated the usefulness of using rflp profiles to examine genetic diversity ...19947909414
the fate of brugia pahangi microfilariae in armigeres subalbatus during the first 48 hours post ingestion.in armigeres subalbatus, 60% and 3% of the ingested brugia pahangi microfilariae (mf) respectively migrated into the haemocoel and the thorax within 5 minutes post ingestion (p.i.). most of the mf had migrated from the gut into the haemocoel within the first 10 minutes p.i. there was no correlation between the number of mf ingested and the migration rate though those in mosquitoes with a low mf burden tend to migrate earlier. at 24 hours p.i., 5-30% of the mf were still in the gut; 19% of these ...19947915044
biting behaviour of armigeres subalbatus (coquillett) with reference to host selection and landing.armigeres subalbatus (coquillett), a vicious crepuscular biter, has been selected to study the biting behaviour with reference to factors affecting landing and host selection in the laboratory. the mosquito showed a higher attractancy to relatively warmer skin of human hands. there was also a significant attraction towards the artificially warmed hands than the normal ones, and the mosquitoes avoided the artificially cooled hands. therefore, host temperature is a factor which influences the attr ...19947927529
urbanization and its effects on the ecology of mosquitoes in macau, southeast asia.recent urbanization in macau has resulted in the precipitous decline to zero in populations of several anopheline vectors of malaria while providing optimal habitat for the population increase in culicines. of 18 species of mosquitoes reported in macau in recent years, 15 species are included here. culex quinquefasciatus, culex sitiens, and aedes albopictus were the most abundant species. notes are provided for anopheles sinensis, armigeres magnus, armigeres subalbatus, culex foliatus, culex fus ...19947707061
studies on adult mosquito vectors of japanese encephalitis in a pig farm in selangor, malaysia.mosquito collections were carried out for a period of one year from january to december 1992 in a pig farm in sungai pelek, selangor, malaysia. a total of 41,022 mosquitos belonging to 52 species and 20 genera were collected. culex tritaeniorhynchus and cx. gelidus, the important vectors, comprised 63% of all mosquitos collected. both these species were collected in large numbers during the wet months of may and december. the other predominant species in that area were cx. fuscocephala, cx. quin ...19947855662
circadian rhythm in the biting behaviour of a mosquito armigeres subalbatus (coquillett).the crepuscular biting rhythm of a. subalbatus has been found to be a genuine circadian rhythm. when the entrained biting rhythm is allowed to freerun in constant darkness (dd) and continuous illumination (ll), it persists in dd (tau = 24.36 hr) and also in ll of ca. 0.1 1x (tau-23.82 hr) thus deviating from the strict 24 hr periodicity of the geophysical day. the biting rhythm becomes arrhythmic in ll of 1.0, 10, 0.4, 4, 40 lx even the first cycle damping away.19947916332
a laboratory study on the life cycle and feeding behaviour of arrenurus madaraszi (acari: arrenuridae) parasitizing anopheles mosquitoes.although mosquitoes of several genera were collected from one locality in thiruvananthapuram, kerala state, india, only anopheles spp. carried larval arrenurus madaraszi; 21.5% of the an. hyrcanus and 19.0% of the an. vagus were infested. however, the whole life cycle of arr. madaraszi could be replicated in the laboratory using aedes albopictus, ae. vittatus or armigeres subalbatus as foster hosts. the host specificity observed in the field is therefore ecological, not physiological. in the lab ...19947915101
insecticidal activity of some new synthetic compounds against different mosquito species.insecticidal activity of an organosilane (hoe 84498, oms 3055), organophosphorous (mat 9460, oms 3052) and a synthetic pyrethroid compound (trebon, oms 3002) was evaluated against adult and larval stages of eight species of mosquitoes. insecticidal activity of these compounds was limited to larval stages only. highest activity of hoe 84498 was found against both cx. tritaeniorhynchus and anopheles culicifacies (lc50-0.0065 mg/i), mat 9460 against armigeres subalbatus (lc50-0.00043 mg/i) and treb ...19959163708
field observations on the swarming behavior of armigeres subalbatus (coq) (diptera : culicidae).swarming behavior of armigeres subalbatus was observed in pondicherry, south india. swarming occurred both at dawn and dusk over stationary markers. the duration of swarming was longer at dust (135 minutes) and shorter at dawn (75 minutes). higher intensity in terms of relative density of participating males was observed at dusk when compared to dawn. peak swarming activity was observed at light intensity ranging between 10 and 183 lux. response to low light intensity of 1-10 lux was different d ...19958525406
hemocyte alterations during melanotic encapsulation of brugia malayi in the mosquito armigeres subalbatus.the involvement of hemocytes in melanotic encapsulation reactions against brugia malayi was assessed in armigeres subalbatus. hemocyte populations, epitope changes, phenol oxidase (po) activity, and the presence of an 84-kda polypeptide were investigated in mosquitoes exposed to a b. malayi-infective bloodmeal (= immune-activated), in mosquitoes given a noninfective bloodmeal (= controls), in nonbloodfed mosquitoes (= naive), or in some combination of these. total hemocyte populations in immune- ...19957535848
biochemical pathway of melanotic encapsulation of brugia malayi in the mosquito, armigeres subalbatus.the mosquito, armigeres subalbatus, is naturally resistant to the filarial worm, brugia malayi, and microfilariae (mf) penetrating the midgut are killed by melanotic encapsulation reactions in the hemocoel within 48 h following ingestion. this vector-parasite system was used to assess changes in hemolymph tyrosine, tyrosine derivatives, and catecholamine-metabolizing enzyme activities using high pressure liquid chromatography with electrochemical detection (hplc-ed) during melanotic encapsulatio ...19958595819
aedes aegypti: induced antibacterial proteins reduce the establishment and development of brugia malayi.the effect of host immune activation on the development of brugia malayi in one susceptible and four refractory strains of aedes aegypti and in armigeres subalbatus was assessed. a. aegypti that were immune activated by the injection of saline or bacteria 24 hr before feeding on a b. malayi-infected gerbil had significantly reduced prevalences and mean intensities of infection from those of naive controls when exposed to bloodmeals with low (105 mf/20 microliters) and medium (160 mf/20 microlite ...19968682188
effects of inhibitors of serine protease, phenoloxidase and dopa decarboxylase on the melanization of dirofilaria immitis microfilariae with armigeres subalbatus haemolymph in vitro.the melanization of dirofilaria immitis microfilariae in armigeres subalbatus haemolymph in vitro is a two-step process. firstly, the microfilariae are encased in a transparent capsule, then the capsule material is melanized later. benzamadine hc1 and p-amidinophenylmethanesulfonyl fluoride, both serine protease inhibitors, inhibited the deposition of the transparent capsule material and melanization. diethyldithiocarbamate, a phenoloxidase inhibitor, did not prevent the deposition of the transp ...19979280896
human dirofilariasis caused by dirofilaria (nochtiella) repens in sri lanka.human dirofilariasis due to dirofilaria (nochtiella) repens is a common zoonotic infection in sri lanka. todate 70 cases are on record, and they include 3 expatriates from russia, england and korea, who were undoubtedly infected in sri lanka. around 30-60% of dogs are infected with d. repens in various parts of the country and the mosquito vectors are aedes aegypti, armigeres subalbatus, mansonia uniformis and m. annulifera. unlike in other countries of the old world infection is most common in ...19979802095
comparison of the autogenous potentials of newly-emerged anautogenous female mosquitoes.the autogenous potentials in several species of newly emerged anautogenous female mosquitoes were examined by homoplastic transplantion of resting stage ovaries into newly-emerged females. ovaries from 4-day-old female culex pipiens quinquefasciatus, with follicles at stage ib, were all activated in the newly emerged hosts and deposited some yolk in the oocyte. in addition, some of the implants (5-10%) matured. transplanted c. tritaeniorhynchus ovaries also started to develop beyond the ib resti ...199712769925
development of a comparative genetic linkage map for armigeres subalbatus using aedes aegypti rflp markers.one of the causative agents of lympahtic filariasis is the nematode parasite brugia malayi that requires a competent mosquito vector for its development and transmission. armigeres subalbatus mosquitoes rapidly destroy invading b. malayi microfilariae via a defense response known as melanotic encapsulation. we have constructed a genetic linkage map for this mosquito species using rflp markers from aedes aegypti. this heterologous approach was possible because of the conserved nature of the codin ...19989445486
molecular cloning, characterization and tissue expression of prophenoloxidase cdna from the mosquito armigeres subalbatus inoculated with dirofilaria immitis microfilariae.a cdna encoding mosquito armigeres subalbatus prophenol oxidase (as-pro-po) was obtained by rapid amplification of cdna ends-polymerase chain reaction (race-pcr) after dirofilaria immitis inoculation. the 2205 bp as-pro-po cdna contains a 32 bp 5'-noncoding region, a 2055 bp open reading frame (685 amino acids), and a 118 bp 3'-noncoding region. hydrophobic signal peptide for the endoplasmic reticulum targeting is not found in the nh2-terminal region. two potential copper-binding domains, amino ...19989459427
genomic structure and ecdysone regulation of the prophenoloxidase 1 gene in the malaria vector anopheles gambiae.prophenoloxidase, a melanin-synthesizing enzyme, is considered to be an important arthropod immune protein. in mosquitoes, prophenoloxidase has been shown to be involved in refractory mechanisms against malaria parasites. in our study we used anopheles gambiae, the most important human malaria vector, to characterize the first arthropod prophenoloxidase gene at the genomic level. the complete nucleotide sequence, including the immediate 5' flanking sequence (-855 bp) of the prophenoloxidase 1 ge ...199910611292
isolation of japanese encephalitis virus from mosquitoes collected in northern taiwan between 1995 and 1996.mosquito collections were carried out from may to october in 1995 and 1996 at yingko and sanhsia of taipei county and chunan of miaoli county. a grand total of 13,576 mosquitoes consisting of 13 species in 407 pools were processed and inoculated into aedes albopictus clone c6/36 cell cultures. one hundred thirty seven pools of these showed the presence of viral antigens in the infected c6/36 cell lysates which were identified by the indirect fluorescent antibody test using a monoclonal antibody ...199911561572
larvicidal, adulticidal and repellent effects of kaempferia galanga.four fractions of kaempferia galanga (hexane fraction, dichloromethane fraction 1, dichloromethane fraction 2 and methanolic fraction) were tested for larvicidal activity toward fourth instar culex quinquefasciatus. the hexane fraction was found to exhibit the highest larvicidal effect with the lc50 of 42.33 ppm. testing for adulticidal activity, the hexane fraction did not show any promising adulticidal effect. however, it caused a knockdown effect which might be useful as a repellent. it was t ...199910774653
susceptibility of ten species of mosquito larvae to the parasitic nematode romanomermis iyengari and its development.ten species of mosquitoes (diptera: culicidae) from five genera were exposed to preparasites of the tropical mermithid nematode species romanomermis iyengari (welch) (nematoda: mermithidae), a strain isolated in 1978 from pondicherry. by exposing mosquito larvae during the second instar, nematode infection was invariably lethal, the rate being highest in culex sitiens wiedemann (95%) followed by cx. quinquefasciatus say (90%), aedes aegypti (l.) (79%), anopheles subpictus grassi (64%), ae. albop ...200011129707
genetics of mosquito vector competence.mosquito-borne diseases are responsible for significant human morbidity and mortality throughout the world. efforts to control mosquito-borne diseases have been impeded, in part, by the development of drug-resistant parasites, insecticide-resistant mosquitoes, and environmental concerns over the application of insecticides. therefore, there is a need to develop novel disease control strategies that can complement or replace existing control methods. one such strategy is to generate pathogen-resi ...200010704476
wolbachia infection and expression of cytoplasmic incompatibility in armigeres subalbatus (diptera: culicidae).polymerase chain reaction screening revealed that armigeres subalbatus (coquillett), a vector of filariasis, was infected with the intracellular bacteria wolbachia. laboratory crosses between infected males and uninfected females resulted in less than half the number of offspring than control crosses between uninfected individuals when young (2- to 3-d-old) males were used in the cross. however, imcompatibility was lost when old (14- to 17-d-old) males were used. field-collected females did not ...200015218907
potential role of armigeres subalbatus (diptera: culicidae) in the transmission of japanese encephalitis virus in the absence of rice culture on liu-chiu islet, taiwan.mosquitoes known to be involved in the transmission of japanese encephalitis virus (je) on taiwan typically develop in rice fields. however, recent serological evidence indicated that je virus was being transmitted on liu-chiu, a rice-free islet. to identify the mosquito vector in this unusual epidemiological situation, 4 mosquito species commonly found in liu-chiu were evaluated for their vector competence for a strain of je (ch1392) virus isolated from central taiwan. armigeres subalbatus (coq ...200015218913
characterization of an endogenous gene expressed in aedes aegypti using an orally infectious recombinant sindbis virus.sindbis virus expression vectors have been used successfully to express and silence genes of interest in vivo in several mosquito species, including aedes aegypti, ae. albopictus, ae. triseriatus,culex pipiens, armigeres subalbatus and anopheles gambiae. here we describe the expression of an endogenous gene, defensin, in ae. aegypti using the orally infectious sindbis virus, mre/3'2j expression vector. we optimized conditions to infect mosquito larvae per os using c6/36ae. albopictus cells infec ...200115455070
molecular cloning of two prophenoloxidase genes from the mosquito aedes aegypti.the biosynthesis of melanotic materials is an important process in the life of a mosquito. melanin production is critical for many diverse processes such as egg chorion tanning, cuticular sclerotization, and melanotic encapsulation of metazoan parasites. prophenoloxidase plays a critical role in this biochemical cascade. two cdnas, one full length and one partial clone, and two genomic clones encoding prophenoloxidase (pro-po) were isolated from the yellow fever mosquito, aedes aegypti. the full ...200111240641
susceptibility of mosquitoes in central taiwan to natural infections of dirofilaria immitis.from october 1997 to september 1998, 3085 culex quinquefasciatus (say) (diptera: culicidae), 584 cx. tritaeniorhynchus (giles) (diptera: culicidae), 392 cx. annulus (theobald) (diptera: culicidae), 374 aedes albopictus (skuse) (diptera: culicidae) and 102 armigeres subalbatus (coquillet) (diptera: culicidae) were collected and examined for dirofilaria immitis (leidy) (spirurida: filariidae) infection. however, only cx. quinquefasciatus and ae. albopictus were infected, with a prevalence of 4.28% ...200111297103
induction of oogenesis in mosquitoes (diptera: culicidae) by infusion of the hemocoel with amino acids.as done previously with adult females of culex pipiens pallens coquillett, a mixture of 17 amino acids was infused into the hemocoel of females of seven anautogenous and one autogenous mosquito species belonging to three genera. in culex. p. quinquefasciatus say, cx. tritaeniorhynchus giles, cx. kyotoensis yamaguti & lacasse, aedes albopictus (skuse), armigeres subalbatus (coquillett), and cx. p. molestus forskal, which previously had laid autogenously matured first batch of eggs, ovarian develo ...200111476338
effect of prophenoloxidase expression knockout on the melanization of microfilariae in the mosquito armigeres subalbatus.melanization is an effective defence reaction used by mosquito hosts to kill malarial and filarial worm parasites. although phenoloxidase (po) has long been considered to be the key enzyme in the biosynthesis of melanotic material in insects, there is no direct evidence verifying its role in parasite melanization. to elucidate the role of po in the melanization of microfilariae (mf) by mosquitoes, a double subgenomic sindbis (dssin) recombinant virus was used to transduce armigeres subalbatus mo ...200111520354
electron microscopic observations on wound-healing in larvae of the mosquito armigeres subalbatus (diptera: culicidae).the wound-healing processes in the mosquito armigeres subalbatus (coquillett) were observed with electron microscopy. the initial reaction involved wound contractions and aggregation of injured surface tissues, cell debris and movement of granulocytes toward the wound. granulocytes first aggregated around the surface of the wound and many filamentous filopodia protruded to connect with cytoplasmic strands. these strands were then interconnected to form a network coagulum resulting in wound closu ...200111761382
molecular cloning of a second prophenoloxidase cdna from the mosquito armigeres subalbatus: prophenoloxidase expression in blood-fed and microfilariae-inoculated mosquitoes.melanization constitutes an important component in various aspects of insect life, including cuticular sclerotization, egg-shell tanning, melanization of parasites and wound healing. recently, a cdna encoding prophenoloxidase (pro-po), a key enzyme in the biosynthesis of melanotic material in insects, was cloned from microfilariae (mf)-inoculated mosquitoes, armigeres subalbatus. however, results of northern blot analyses indicated that two pro-pos might be present in ar. subalbatus and these pr ...200111240640
immunolocalization of prophenoloxidase in the process of wound healing in the mosquito armigeres subalbatus (diptera: culicidae).hemolymph coagulation began almost immediately after wounding in mosquito, armigeres subalbatus, (coquillett) larvae. immunocytochemical localization showed that prophenoloxidase (pro-po) was distributed in the wound site. in the initial wounding, coagulation and wound plug formation occurred with granulocyte migration. the hemocytes lysed and released granular materials around the wound site, prophenoloxidase being mostly localized in granules and cuticle. in the second phase of wound healing, ...200211931025
abstracts of the fourth international symposium on molecular insect science. may 28-june 2, 2002. tucson, arizona, usa. 200215455051
reproductive aspects of the mosquito culex quinquefasciatus (diptera:culicidae) infected with wuchereria bancrofti (spirurida: onchocercidae).this study reports on the relationship between wuchereria bancrofti infection and female body size, intake of blood and fecundity in the mosquito culex quinquefasciatus, vector of this filarial parasite in recife (brazil). adults from field collected larvae were infected via a membrane feeding procedure, using blood with parasitaemia ranging from 724-6,000 mf/ml. a positive correlation was observed between mosquito size (measured by wing length) and egg production in uninfected females. however, ...200312764437
hemocyte-mediated phagocytosis and melanization in the mosquito armigeres subalbatus following immune challenge by bacteria.mosquitoes are important vectors of disease. these insects respond to invading organisms with strong cellular and humoral immune responses that share many similarities with vertebrate immune systems. the strength and specificity of these responses are directly correlated to a mosquito's ability to transmit disease. in the current study, we characterized the hemocytes (blood cells) of armigeres subalbatus by morphology (ultrastructure), lectin binding, enzyme activity, immunocytochemistry, and fu ...200312838409
repellency of aromatic turmeric curcuma aromatica under laboratory and field conditions.three curcuma species, curcuma aeruginosa (pink and blue ginger), cu. aromatica (aromatic turmeric), and cu. xanthorrhiza (giant curcuma), were selected for investigation of mosquito repellent activity. in a laboratory study, a 95% ethanol extract of each plant was tested for repellent activity of aedes togoi on human volunteers. only cu. aromatica extract showed repellency against ae. togoi with ed50 and ed95 values of 0.061 and 1.55 mg/cm2, respectively. it also provided biting protection for ...200314714673
short report: detection of japanese encephalitis virus in mouse peripheral blood mononuclear cells using an in situ reverse transcriptase-polymerase chain reaction.japanese encephalitis (je) is an important mosquito-borne viral disease in southeast asia. isolation of je virus from peripheral blood is usually difficult because of transient and low titer of viremia. an in situ reverse transcriptase-polymerase chain reaction (rt-pcr) method was designed to amplify gene (envelope) fragments of je virus residing in peripheral blood mononuclear cells (pbmcs) without extraction of rna. baby hamster kidney-21 cells infected with the t1p1 strain of je virus (an iso ...200314740883
a novel lectin with a fibrinogen-like domain and its potential involvement in the innate immune response of armigeres subalbatus against bacteria.mosquitoes have an efficient cellular innate immune response that includes phagocytosis of microbial pathogens and encapsulation of metozoan parasites. in this study, we describe a novel lectin in the mosquito, armigeres subalbatus (aslectin or al-1). the 1.27 kb cdna clone for the al-1 gene (al-1) encodes a 279 deduced amino acid sequence that contains a c-terminal fibrinogen-like domain. al-1 is transcribed in all life stages. al-1 mainly exists in the haemolymph of adult female mosquitoes, an ...200415157228
mosquito repellency of the seeds of celery (apium graveolens l.).when the mosquito repellencies of four fractions of apium graveolens seeds (one hexane, two dichloromethane and one methanolic) were investigated in the laboratory, all four were found to offer human volunteers some protection against female, adult aedes aegypti. the hexane fraction, however, was found to exhibit the highest repellency in the laboratory, with median effective doses (ed50) and ed95 of 0.41 and 2.93 mg/cm2 skin, respectively. only this fraction, which was also found to provide pro ...200415228722
molecular (sub) grouping of endosymbiont wolbachia infection among mosquitoes of taiwan.wolbachia are maternally inherited bacteria that infect a wide range of arthropods as well as filarial worms. the infection usually results in reproductive distortions of the host, primarily cytoplasmic incompatibility, parthenogenesis, and feminization. this study showed that wolbachia infection (15/29; 51.72%) was prevalent among field-caught mosquitoes in taiwan. three mosquito species were identified as having wolbachia a infection, eight species as having wolbachia b, and four other species ...200415311460
yeast-generated co2 as a convenient source of carbon dioxide for adult mosquito sampling.a new, convenient method was developed to supply co2 for mosquito sampling by using yeast, which converts sugar into co2 and ethyl alcohol. the system could, at average, generate 32.4 ml/min of co2 for at least 27 h. the total weight of the co2 generated was estimated to be 94 g. the efficacy of yeast-generated co2 as attractant for mosquitoes was significant, and the following 6 mosquito species were collected using yeast-generated co2 traps from july to september 2003 in a residential area of ...200415532924
the role of phenylalanine hydroxylase in melanotic encapsulation of filarial worms in two species of mosquitoes.melanin formation has a significant influence on mosquito vector competence by limiting the development of metazoan parasites. tyrosine, the rate-limiting substrate of melanin production, can be obtained exogenously or derived from phenylalanine by phenylalanine hydroxylase (pah). the characteristics of this defense mechanism, such as temporal expression of constituent enzymes involved in the biosynthetic pathway, can vary considerably between mosquito species. we investigated the functional rol ...200415544946
the use of a double subgenomic sindbis virus expression system to study mosquito gene function: effects of antisense nucleotide number and duration of viral infection on gene silencing efficiency.recently we established a simple, effective antisense strategy using a double subgenomic sindbis (dssin) virus expression system to study gene function in mosquitoes. in this study, we further elucidate the effects of antisense nucleotide number and duration of viral infection on mosquito gene silencing efficiency by the dssin virus expression system. over 15 days post virus infection, the degree of parasite melanization was progressively reduced by more than 95%, 75% and 55% in the mosquito arm ...200415606808
mosquito innate immunity: involvement of beta 1,3-glucan recognition protein in melanotic encapsulation immune responses in armigeres subalbatus.beta 1,3-glucan recognition proteins (grp) have specific affinity for beta 1,3-glucan, a component on the surface of fungi and bacteria. by interacting with beta 1,3-glucan, grp initiates activation of prophenoloxidase, a key enzyme in the signaling pathway leading to melanotic encapsulation in invertebrates. in this study, we characterize a novel hemocyte-specific grp from the mosquito, armigeres subalbatus (asgrp). the 1.57 kb cdna clone encodes a 499 deduced amino acid sequence, which contain ...200515610820
characterization of two novel pacifastin-like peptide precursor isoforms in the desert locust (schistocerca gregaria): cdna cloning, functional analysis and real-time rt-pcr gene expression studies.in the last decade, a new serine protease inhibitor family has been described in arthropods. eight members of the family were purified from locusts and share a conserved cysteine array (cys-xaa(9-12)-cys-asn-xaa-cys-xaa-cys-xaa(2-3)-gly-xaa(3-6)-cys-thr-xaa3-cys) with nine inhibitory domains of the light chain of the crayfish protease inhibitor, pacifastin (plds; pacifastin light chain domains). using cdna cloning, several pacifastin-related precursors have been identified, encoding additional p ...200515631618
quantitative genetics of vector competence for la crosse virus and body size in ochlerotatus hendersoni and ochlerotatus triseriatus interspecific hybrids.la crosse virus is a leading cause of pediatric encephalitis in the united states. the mosquito ochlerotatus triseriatus is an efficient vector for la crosse virus, whereas the closely related o. hendersoni transmits only at very low rates. quantitative trait loci (qtl) affecting the ability to orally transmit this virus and adult body size were identified in 164 f(2) female individuals from interspecific crosses of o. hendersoni females and o. triseriatus males using a combination of composite ...200515654112
manduca sexta prophenoloxidase activating proteinase-1 (pap-1) gene: organization, expression, and regulation by immune and hormonal signals.insect phenoloxidase (po) participates in melanotic encapsulation, wound healing, and cuticle sclerotization. it is converted from prophenoloxidase (propo) by a propo-activating proteinase (pap). manduca sexta pap-1, the final component of a serine proteinase cascade, cleaves propo to generate active po. in an effort to understand the transcriptional regulation, we isolated a genomic clone of the pap-1 gene, determined its nucleotide sequence, and elucidated its exon-intron organization. compute ...200515857768
the use of gene silencing to study the role of dopa decarboxylase in mosquito melanization reactions.mosquito melanization involves hydroxylation of tyrosine to dopa, which then is oxidized to dopaquinone by phenoloxidase, or decarboxylated to dopamine by dopa decarboxlase (ddc). an armigeres subalbatus cdna encoding ddc was cloned and real-time pcr analysis revealed increased transcripts in blood-fed and microfilariae (mf)-inoculated mosquitoes. a double subgenomic sindbis virus was used to silence ddc and assess its role in melanization of mf. ddc transcription and activity were significantly ...200515926892
frequency-dependent prey-selection of predacious water bugs on armigeres subalbatus immatures.the predatory behaviour with reference to the frequency-dependent prey-selection of the water bugs sphaerodema annulatum fabricius and s. rusticum fabricius was studied in the laboratory using the iv instar larvae and pupae of armigeres subalbatus as prey to ascertain their efficacy as predator of mosquito immatures.200515999455
identification and characterization of the fibrinogen-like domain of fibrinogen-related proteins in the mosquito, anopheles gambiae, and the fruitfly, drosophila melanogaster, genomes.the fibrinogen-like (fbg) domain, which consists of approximately 200 amino acid residues, has high sequence similarity to the c-terminal halves of fibrinogen beta and gamma chains. fibrinogen-related proteins (freps), which contain fbg domains in their c-terminal region, are found universally in vertebrates and invertebrates. in invertebrates, freps are involved in immune responses and other aspects of physiology. to understand the complexity of this family in insects, we analyzed freps in the ...200516150145
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