Publications

leifsonia gen. nov., a genus for 2,4-diaminobutyric acid-containing actinomycetes to accommodate "corynebacterium aquaticum" leifson 1962 and clavibacter xyli subsp. cynodontis davis et al. 1984.	"corynebacterium aquaticum" was first proposed by leifson in 1962 but not included in the approved lists of bacterial names in 1980. this species has been left from reclassification of the genus corynebacterium because of the unusual chemotaxonomic characteristics such as 2,4-diaminobutyric acid (dab) in the peptidoglycan and menaquinones of mk-10 and mk-11. a close relationship of "c. aquaticum" to the genera agromyces and rathayibacter has been pointed out from the viewpoint of chemotaxonomic ...	1999	12501368
leifsonia poae gen. nov., sp. nov., isolated from nematode galls on poa annua, and reclassification of 'corynebacterium aquaticum' leifson 1962 as leifsonia aquatica (ex leifson 1962) gen. nov., nom. rev., comb. nov. and clavibacter xyli davis et al. 1984 with two subspecies as leifsonia xyli (davis et al. 1984) gen. nov., comb. nov.	the new genus leifsonia gen. nov. with two new species, leifsonia poae sp. nov. (type strain vkm ac-1401t) and leifsonia aquatica (ex leifson 1962) nom. rev., comb. nov. (the type species, with vkm ac-1400t = dsm 20146t = jcm 1368t as type strain), is proposed to accommodate bacteria found in poa annua root gall, induced by the nematode subanguina radicicola, and 'corynebacterium aquaticum' leifson 1962. further, it is proposed to reclassify clavibacter xyli davis et al. 1984 with two subspecies ...	2000	10826825
transformation and transposon mutagenesis of leifsonia xyli subsp. xyli, causal organism of ratoon stunting disease of sugarcane.	conditions have been developed for genetic transformation and insertional mutagenesis in leifsonia xyli subsp. xyli (lxx), the causal organism of ratoon stunting disease (rsd), one of the most damaging and intractable diseases of sugarcane internationally. transformation frequencies ranged from 1 to 10 colony forming units (cfu)/microg of plasmid dna using clavibacter/escherichia coli shuttle vectors pcg188, pdm302, and pdm306 and ranged from 50 to 500 cfu/microg using cosmid cloning vectors pla ...	2002	11952129
leifsonia rubra sp. nov. and leifsonia aurea sp. nov., psychrophiles from a pond in antarctica.	two unique psychrophilic strains (cms 76rt and cms 81yt) were isolated from a cyanobacterial mat sample from a pond in wright valley, mcmurdo, antarctica. both isolates were assigned to the genus leifsonia, since they were gram-positive, curved rods, non-motile, catalase-positive, contained dl-2,4-diaminobutyric acid, menaquinone mk-11, phosphatidylglycerol and diphosphatidylglycerol, had a high content of anteiso- and iso-branched fatty acids and had a dna g + c content of 64-66 mol%. in additi ...	2003	12892114
rhodoglobus vestalii gen. nov., sp. nov., a novel psychrophilic organism isolated from an antarctic dry valley lake.	a novel, psychrophilic, gram-positive bacterium (designated strain lv3t) from a lake near the mcmurdo ice shelf, antarctica, has been isolated and characterized. this organism formed red-pigmented colonies, had an optimal growth temperature of 18 degrees c and grew on a variety of media between -2 and 21 degrees c. scanning electron micrographs of strain lv3t that showed small rods with unusual bulbous protuberances during all phases of growth were of particular interest. the g + c content of th ...	2003	12892115
establishment of a functional genomics platform for leifsonia xyli subsp. xyli.	leifsonia xyli subsp. xyli, the causal agent of ratoon stunting disease in sugarcane, is a xylem-limited, nutritionally fastidious, slow growing, gram-positive coryneform bacterium. because of the difficulties in growing this bacterium in pure culture, little is known about the molecular mechanisms of pathogenesis. currently, the genome sequence of l. xyli subsp. xyli is being completed by the agronomical and environmental genomes group from the organization for nucleotide sequencing and analysi ...	2004	14964531
characterization of the replicon of a 51-kb native plasmid from the gram-positive bacterium leifsonia xyli subsp. cynodontis.	the 4992-bp replicon of a large cryptic plasmid in the gram-positive bacterium leifsonia xyli subsp. cynodontis was identified and sequenced. the replicon encoded two proteins essential for plasmid replication and stability. the putative replication protein (repa) is homologous to that of the plasmids in mycobacterial plr7 family, while the putative para protein immediately downstream of repa is significantly homologous to the walker-type atpase required for partition of plasmid and chromosome o ...	2004	15212787
the genome sequence of the gram-positive sugarcane pathogen leifsonia xyli subsp. xyli.	the genome sequence of leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. the single circular chromosome of leifsonia xyli subsp. xyli ctcb07 was 2.6 mb in length with a gc content of 68% and 2,044 predicted open reading frames. the analysis also revealed 307 predicted pseudogenes, which is more than any bacterial plant pathogen sequenced to date. many of these pseudogenes, if functional, would likely be involved in the degradation o ...	2004	15305603
bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations.	heterotrophic populations were isolated and characterized from an alkaline groundwater environment generated by active serpentinization, which results in a ca(oh)2-enriched, extremely diluted groundwater with ph 11.4. one hundred eighty-five strains were isolated in different media at different ph values during two sampling periods. to assess the degree of diversity present in the environment and to select representative strains for further characterization of the populations, we screened the is ...	2004	15574939
purification and characterization of a novel alcohol dehydrogenase from leifsonia sp. strain s749: a promising biocatalyst for an asymmetric hydrogen transfer bioreduction.	to find microorganisms that could reduce phenyl trifluoromethyl ketone (ptk) to (s)-1-phenyltrifluoroethanol [(s)-pte], styrene-assimilating bacteria (ca. 900 strains) isolated from soil samples were screened. we found that leifsonia sp. strain s749 was the most suitable strain for the conversion of ptk to (s)-pte in the presence of 2-propanol as a hydrogen donor. the enzyme corresponding to the reaction was purified homogeneity, characterized and designated leifsonia alcohol dehydrogenase (lsad ...	2005	16000771
microcella putealis gen. nov., sp. nov., a gram-positive alkaliphilic bacterium isolated from a nonsaline alkaline groundwater.	three gram-positive bacteria designated cv-2t, cv-40 and ac-30 were isolated from a highly alkaline groundwater environment (ph 11.4). these organisms formed very small rod-shaped cells, are aerobic, non-spore-forming, catalase-positive, oxidase-negative, with an optimum growth temperature of 35 degrees c and optimum ph value of growth between 8.5 and 9.0. the strains possessed a novel b-type cell-wall peptidoglycan structure with lysine as the diamino acid; the major respiratory quinones were m ...	2005	16104348
[isoprenoid pigments in representatives of the family microbacteriaceae].	by using fosmidomycin and mevinolin (inhibitors of the synthesis of isoprenoid pigments), spectrophotometry, and mass spectrometry, the presence of isoprenoid pigments is shown in 71 of the 78 strains under study. all of these strains belong to 11 genera of the family microbacteriaceae. yellow, orange, and red pigments are found to have absorption spectra typical of c40-carotenoids. eight out of the sixteen strains of the genus microbacterium are able to synthesize neurosporene, a precursor of l ...	2005	16119846
bacterial phytopathogens and genome science.	there are now fourteen completed genomes of bacterial phytopathogens, all of which have been generated in the past six years. these genomes come from a phylogenetically diverse set of organisms, and range in size from 870 kb to more than 6mb. the publication of these annotated genomes has significantly helped our understanding of bacterial plant disease. these genomes have also provided important information about bacterial evolution. examples of recently completed genomes include: pseudomonas s ...	2005	16125997
[from the mailing list sin: epidemic of infections caused by 'aquatic' bacteria in patients undergoing hemodialysis via central venous catheters].	bacteremia due to central venous catheter (cvc) infection is the most frequent complication of cvc use as vascular access for hemodialysis (hd). we report a case of an epidemic of cvc infections caused by 3 strains of unusual bacteria: ralstonia pickettii (rp), leifsonia xyli/leifsonia aquatica (lxa), tsuckamurella strandjordae (ts). from 20/8/01 to 30/9/01, 23 of 35 patients dialyzed via cvcs experienced intra-hd pyrogenic reactions. their hemocultures were positive for: rp (14 pts), lxa (3 pts ...	2005	16267809
gene cloning and expression of leifsonia alcohol dehydrogenase (lsadh) involved in asymmetric hydrogen-transfer bioreduction to produce (r)-form chiral alcohols.	the gene encoding leifsonia alcohol dehydrogenase (lsadh), a useful biocatalyst for producing (r)-chiral alcohols, was cloned from the genomic dna of leifsonia sp. s749. the gene contained an opening reading frame consisting of 756 nucleotides corresponding to 251 amino acid residues. the subunit molecular weight was calculated to be 24,999, which was consistent with that determined by polyacrylamide gel electrophoresis. the enzyme was expressed in recombinant escherichia coli cells and purified ...	2006	16495658
signature proteins that are distinctive characteristics of actinobacteria and their subgroups.	the actinobacteria constitute one of the main phyla of bacteria. presently, no morphological and very few molecular characteristics are known which can distinguish species of this highly diverse group. in this work, we have analyzed the genomes of four actinobacteria (viz. mycobacterium leprae tn, leifsonia xyli subsp. xyli str. ctcb07, bifidobacterium longum ncc2705 and thermobifida fusca yx) to search for proteins that are unique to actinobacteria. our analyses have identified 233 actinobacter ...	2006	16670965
lateral transfers of serine hydroxymethyltransferase (glya) and udp-n-acetylglucosamine enolpyruvyl transferase (mura) genes from free-living actinobacteria to the parasitic chlamydiae.	the chlamydiae are important human and animal pathogens which form a phylogentically distinct lineage within the bacteria. there is evidence that some genes in these obligate intracellular parasites have undergone lateral exchange with other free-living organisms. in the present work, we describe two interesting cases of lateral gene transfer between chlamydiae and actinobacteria, which have been identified based on the shared presence of conserved inserts in two important proteins. in the enzym ...	2006	16830093
a type ib parb protein involved in plasmid partitioning in a gram-positive bacterium.	our current understanding of segregation of prokaryotic plasmids has been derived mainly from the study of the gram-negative bacterial plasmids. we previously reported a replicon of the cryptic plasmid from a gram-positive bacterium, leifsonia xyli subsp. cynodontis. the replicon contains a putative plasmid partition cassette including a walker-type atpase followed by open reading frame 4 without sequence homologue. here we reported that the orf4 gene was essential for maintaining the plasmid st ...	2006	16997970
secrets of soil survival revealed by the genome sequence of arthrobacter aurescens tc1.	arthrobacter sp. strains are among the most frequently isolated, indigenous, aerobic bacterial genera found in soils. member of the genus are metabolically and ecologically diverse and have the ability to survive in environmentally harsh conditions for extended periods of time. the genome of arthrobacter aurescens strain tc1, which was originally isolated from soil at an atrazine spill site, is composed of a single 4,597,686 basepair (bp) circular chromosome and two circular plasmids, ptc1 and p ...	2006	17194220
[evaluation of api coryne system, version 2.0, for diphteroid gram-positive rods identification with clinical relevance].	the ability of the api coryne system, version 2.0, to identify 178 strains of gram-positive rods was evaluated. seventy eight isolates belonged to genus corynebacterium and one hundred to related genera, all strains were isolated from clinical samples at the laboratory of bacteriology, hospital de clínicas josé de san martin (uba) between 1995 and 2004. the isolates were identified according to von graevenitz and funke's scheme. one hundred and sixty two out of 178 strains (91%) were correctly i ...	2006	17370571
continuous production of chiral 1,3-butanediol using immobilized biocatalysts in a packed bed reactor: promising biocatalysis method with an asymmetric hydrogen-transfer bioreduction.	an asymmetric hydrogen-transfer biocatalyst consisting of mutated rhodococcus phenylacetaldehyde reductase (par) or leifsonia alcohol dehydrogenase (lsadh) was applied for some water-soluble ketone substrates. among them, 4-hydroxy-2-butanone was reduced to (s)/(r)-1,3-butanediol, a useful intermediate for pharmaceuticals, with a high yield and stereoselectivity. intact escherichia coli cells overexpressing mutated par (sar268) or lsadh were directly immobilized with polyethyleneimine or 1,6-dia ...	2007	17443321
the status of the species leifsonia rubra reddy et al. 2003. request for an opinion.	on the basis of 16s rrna gene sequence analysis, it is ascertained that the type strain of leifsonia rubra does not exist in any established culture collection or with the authors who described this species. therefore, it cannot be included in any scientific study. it is proposed that the judicial commission place the name leifsonia rubra on the list of rejected names if a suitable type strain is not found or a neotype is not proposed within two years following the publication of this request fo ...	2007	17473276
identification of bacteria on the surface of clinically infected and non-infected prosthetic hip joints removed during revision arthroplasties by 16s rrna gene sequencing and by microbiological culture.	it has been postulated that bacteria attached to the surface of prosthetic hip joints can cause localised inflammation, resulting in failure of the replacement joint. however, diagnosis of infection is difficult with traditional microbiological culture methods, and evidence exists that highly fastidious or non-cultivable organisms have a role in implant infections. the purpose of this study was to use culture and culture-independent methods to detect the bacteria present on the surface of prosth ...	2007	17501992
biodegradation of imidacloprid by an isolated soil microorganism.	imidacloprid (1-[(6-chloro-3-pyridinyl)-methyl]-n-nitro-2-imidazolidinimine), a chloronicotinyl insecticide used to control biting and sucking insects, is very persistent in the soil with a half-life often greater than 100 days. although a few soil metabolites have been reported in the literature, there are no reports of imidacloprid-degrading soil microorganisms. our objectives were to discover, isolate, and characterize microorganisms capable of degrading imidacloprid in soil. two soil-free st ...	2007	17562458
leifsonia ginsengi sp. nov., isolated from ginseng root.	a gram-positive, rod-shaped, non-motile bacterium, designated strain wged11t, was isolated from the root of ginseng, and its taxonomic position was established using a polyphasic approach. phylogenetic analysis based on 16s rrna gene sequences showed that this organism formed a robust clade with recognized species of the genus leifsonia. strain wged11t was characterized by a high content of omega-cyclohexylundecanoic and anteiso- and iso-branched saturated fatty acids, mk-11 as the major menaqui ...	2007	17267987
characterization of the variants, flanking genes, and promoter activity of the leifsonia xyli subsp. cynodontis insertion sequence is1237.	we performed a comprehensive study of the distribution and function of an insertion sequence (is) element, is1237, in the genome of leifsonia xyli subsp. cynodontis, a useful genetic carrier for expressing beneficial foreign genes in plants. two shorter is1237 isoforms, is1237d1 and is1237d2 resulting from precise deletion between two nonperfect repeats, were found in the bacterial genome at a level that was one-fifth the level of wild-type is1237. both the genome and native plasmid pcxc100 harb ...	2007	17293427
putative lipoproteins identified by bioinformatic genome analysis of leifsonia xyli ssp. xyli, the causative agent of sugarcane ratoon stunting disease.	summary leifsonia xyli ssp. xyli is the causative agent of ratoon stunting disease, a major cause of economic loss in sugarcane crops. understanding of the biology of this pathogen has been hampered by its fastidious growth characteristics in vitro. however, the recent release of a genome sequence for this organism has allowed significant novel insights. further to this, we have performed a bioinformatic analysis of the lipoproteins encoded in the l. xyli genome. these analyses suggest that lipo ...	2007	20507484
construction of a stable expression vector for leifsonia xyli subsp. cynodontis and its application in studying the effect of the bacterium as an endophytic bacterium in rice.	to study the possibility of utilizing genetically engineered leifsonia xyli subsp. cynodontis (lxc) as an endophytic bacterium in rice, we constructed an escherichia coli-lxc shuttle vector, plgus, containing a beta-glucuronidase reporter gene, which was stable both in vitro and in vivo. lxc grows and expresses the beta-glucuronidase reporter gene in all parts of rice, except for seed. a 2-year field study using three rice varieties from china showed that lxc inoculation did not have a negative ...	2007	17166228
genomics of actinobacteria: tracing the evolutionary history of an ancient phylum.	actinobacteria constitute one of the largest phyla among bacteria and represent gram-positive bacteria with a high g+c content in their dna. this bacterial group includes microorganisms exhibiting a wide spectrum of morphologies, from coccoid to fragmenting hyphal forms, as well as possessing highly variable physiological and metabolic properties. furthermore, actinobacteria members have adopted different lifestyles, and can be pathogens (e.g., corynebacterium, mycobacterium, nocardia, tropherym ...	2007	17804669
central venous catheter-associated leifsonia aquatica bacteremia in a hemodialysis-dependent patient.	infections associated with leifsonia aquatica are particularly uncommon. we describe a central venous catheter-associated l. aquatica bacteremia in a hemodialysis-dependent patient. a review of the literature revealed only 1 other case report involving 10 hemodialysis patients with documented l. aquatica bacteremia.	2008	18243628
thermally treated grass fibers as colonizable substrate for beneficial bacterial inoculum.	this study investigates how thermally treated (i.e., torrefied) grass, a new prospective ingredient of potting soils, is colonized by microorganisms. torrefied grass fibers (tgf) represent a specific colonizable niche, which is potentially useful to establish a beneficial microbial community that improves plant growth. tgf and torrefied grass extracts (tge) were inoculated with a suspension of microorganisms obtained from soil. sequential microbial enrichment steps were then performed in both su ...	2008	18368438
humibacter albus gen. nov., sp. nov., isolated from sewage sludge compost.	a bacterial strain isolated from sewage sludge compost, strain sc-083t, was characterized. the isolate was a motile, gram-positive, short rod, forming coryneform v-shaped cells during the early stages of growth. the organism was strictly aerobic and able to grow between 22 and 36 degrees c and between ph 5.5 and 8.0. the predominant fatty acids were cyclohexyl-c17 : 0, anteiso-c17 : 0 and iso-c16 : 0, the major respiratory quinones were menaquinone 11 (mk-11) and 12 (mk-12), and the genomic dna ...	2008	18398211
phycicola gilvus gen. nov., sp. nov., an actinobacterium isolated from living seaweed.	a gram-positive, aerobic, non-mycelium-forming actinomycete, designated strain ssww-21(t), was isolated from a living seaweed sample on the coast of jeju, republic of korea. cells of the organism were rod-shaped or coccoid depending on culture age. short rods were observed in young cultures, whereas older cultures predominantly consisted of coccoid cells. rod-shaped cells were motile by means of flagella, but coccoid cells were non-motile. budding-like cell division was observed. the temperature ...	2008	18523172
bacterial diversity of taxus rhizosphere: culture-independent and culture-dependent approaches.	the regional variability of taxus rhizosphere bacterial community composition and diversity was studied by comparative analysis of three large 16s rrna gene clone libraries from the taxus rhizosphere in different regions of china (subtropical and temperate regions). one hundred and forty-six clones were screened for three libraries. phylogenetic analysis of 16s rrna gene sequences demonstrated that the abundance of sequences affiliated with gammaproteobacteria, betaproteobacteria, and actinobact ...	2008	18576948
characterization of new is elements and studies of their dispersion in two subspecies of leifsonia xyli.	leifsonia xyli is a xylem-inhabiting bacterial species comprised of two subspecies: l. xyli subsp. xyli (lxx) and l. xyli subsp. cynodontis (lxc). lxx is the causal agent of ratoon stunting disease in sugarcane commercial fields and lxc colonizes the xylem of several grasses causing either mild or no symptoms of disease. the completely sequenced genome of lxx provided insights into its biology and pathogenicity. since is elements are largely reported as an important source of bacterial genome di ...	2008	18655699
leifsonia bigeumensis sp. nov., isolated from soil on bigeum island, korea.	a gram-positive, motile, mesophilic bacterium that formed yellowish-white, circular, convex, glistening colonies, strain msl-27t, was isolated from a soil sample from bigeum island, korea, and its taxonomic position was investigated by a polyphasic approach. cells of strain msl-27t are non-spore-forming, irregular rods, usually fragmenting into shorter rods or coccoid elements. the growth temperature and ph ranges are 20-37 degrees c and ph 6.0-12.0, with optimum growth at 28 degrees c and ph 8. ...	2008	18676484
a novel toxin-antitoxin operon tala/b from the gram-positive bacterium leifsonia xyli subsp. cynodontis.	here we report a toxin-antitoxin (ta) operon talab identified from the gram-positive bacterium leifsonia xyli subsp. cynodontis. it is shown that talb encodes a broad-host cytotoxin functioning in different gram-positive bacteria, while tala encodes its antidote. tala and talb form different hetero-oligomers in vitro; these hetero-oligomers, but not the antitoxin tala, strongly bind to the talab promoter region containing two inverted repeats. this represents a new mechanism of binding the promo ...	2008	18722371
[purification and characterization of a beta-d-xylosidase from leifsonia shinshuensis dicp 16].	a beta-d-xylosidase from leifsonia shinshuensis dicp 16 was purified to apparent homogeneity using a combination of ammonium sulfate precipitation, de 52 anion-exchange, q-sepharose fast flow anion-exchange, toyopearl butyl 650c hydrophobic-interaction and sephacryl s-300 hr gel-permeation chromatography. the purified xylosidase consisted of two same subunits and had the relative molecular weight of 180 kd as determined by sds-page and gel-permeation chromatography. the maximal beta-d-xylosidase ...	2008	18724710
leifsonia pindariensis sp. nov., isolated from the pindari glacier of the indian himalayas, and emended description of the genus leifsonia.	strain pon10(t) is a yellow-pigmented, gram-positive, aerobic, motile, rod-shaped bacterium isolated from the pindari glacier of the indian himalayas. the cell-wall peptidoglycan contained dl-diaminobutyric acid as the diamino acid. the predominant fatty acids were anteiso-c(15:0), anteiso-c(17:0) and iso-c(16:0) and the major isoprenoid quinones were mk-10 and mk-11. based on the above characteristics, strain pon10(t) was assigned to the genus leifsonia. blast sequence similarity results indica ...	2008	18768634
chemolithoautotrophic oxidation of thiosulfate and phylogenetic distribution of sulfur oxidation gene (soxb) in rhizobacteria isolated from crop plants.	twenty-one thiosulfate-oxidizing bacteria were isolated from rhizosphere soils and 16s rrna analysis revealed that the isolates were affiliated with seven different phylogenetic groups within the beta and gamma subclasses of proteobacteria and actinobacteria. among these, five genera, including dyella, burkholderia, alcaligenes, microbacterium and leifsonia sp., represented new sulfur oxidizers in rhizosphere soils. the thiosulfate-oxidizing dyella, burkholderia, alcaligenes, microbacterium, lei ...	2008	18832027
isolation and characterization of an nad+-degrading bacterium ptx1 and its role in chromium biogeochemical cycle.	microorganisms can reduce toxic chromate to less toxic trivalent chromium [cr(iii)]. besides cr(oh)(3) precipitates, some soluble organo-cr(iii) complexes are readily formed upon microbial, enzymatic, and chemical reduction of chromate. however, the biotransformation of the organo-cr(iii) complexes has not been characterized. we have previously reported the formation of a nicotinamide adenine dinucleotide (nad(+))-cr(iii) complex after enzymatic reduction of chromate. although the nad(+)-cr(iii) ...	2008	17701280
leifsonia lichenia sp. nov., isolated from lichen in japan.	an actinobacterial strain, 2sbt, isolated from lichen was characterized taxonomically using a polyphasic approach. strain 2sbt was gram-positive, strictly aerobic, rod-shaped and non-motile. phylogenetic analysis based on 16s rrna gene sequence revealed that strain 2sbt was located in the genus leifsonia. level of 16s rrna gene sequence similarity between the isolated strains and the type strains of leifsonia species were 95.0-99.2%. the value of dna-dna relatedness between strain 2sbt and leifs ...	2009	19940379
leifsonia kribbensis sp. nov., isolated from soil.	a yellow-pigmented actinobacterium, designated strain msl-13t, was isolated from a soil sample collected from bigeum island, republic of korea, and its taxonomic position was determined by using a polyphasic approach. strain msl-13t showed phenotypic and chemotaxonomic properties consistent with its classification in the genus leifsonia. 16s rrna gene sequence analysis of strain msl-13t with sequences from leifsonia naganoensis db103t, leifsonia aquatica dsm 20146t, leifsonia xyli subsp. cynodon ...	2009	19126716
interactions of plant-beneficial bacteria with the ascomycete coniochaeta ligniaria.	to assess the interactions between coniochaeta ligniaria f/tgf15 obtained from torrefied grass fibers (tgf) and selected bacteria from the same substrate.	2009	19298515
cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system.	soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. we isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. this approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium leifsonia xyli, a plant pathogen that has never been isolated outside the host.	2009	19304839
description of leifsonia kafniensis sp. nov. and leifsonia antarctica sp. nov.	strains kfc-22(t) and spc-20(t) are yellow-pigmented, gram-positive, aerobic, non-motile, rod-shaped bacteria that were isolated from a soil sample near the kafni glacier in the himalayan mountain ranges in india, and from a spade core sediment sample from the antarctic ocean at larsemann hill, respectively. in both cases, the cell-wall peptidoglycan contained 2,4-diaminobutyric acid as the diamino acid, anteiso-c(15 : 0), anteiso-c(17 : 0) and iso-c(16 : 0) were the predominant fatty acids and ...	2009	19502314
bacterial community and nitrogen fixation in the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae).	the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae), colonizes all pines species within its native range throughout north and central america. recently, this species was accidentally introduced to china, where it has caused severe damage in pine forests. it belongs to a group of beetles that spend most of their lives between the tree bark and sapwood, where it feeds on phloem: a poor substrate with very low nutritional value of nitrogen and toxic proper ...	2009	19543937
biocatalytic production of (s)-4-bromo-3-hydroxybutyrate and structurally related chemicals and their applications.	the enzymatic production of (s)-4-bromo-3-hydroxybutyrate has been poorly studied compared with (s)-4-chloro-3-hydroxybutyrate. this can be attributed to the toxicity of bromide for biocatalysis. recently, we isolated cdna that encodes penicillium citrinum beta-keto ester reductase (ker) and the gene that encodes leifsonia sp. alcohol dehydrogenase, which catalyzes the reduction of methyl 4-bromo-3-oxobutyrate to methyl (s)-4-bromo-3-hydroxybutyrate with high optical purity and productivity and ...	2009	19593557
leifsonia soli sp. nov., a yellow-pigmented actinobacterium isolated from teak rhizosphere soil.	two yellow-pigmented, gram-stain-positive, aerobic, motile, short rod-shaped bacteria were isolated from natural teak tree rhizosphere soil and their taxonomic positions were determined by using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that strains tg-s248(t) and tg-s240 formed a distinct phyletic line within the genus leifsonia. 16s rrna gene sequence analysis of strain tg-s248(t) with sequences from leifsonia shinshuensis db 102(t), l. poae vkm ac-1401(t), l. n ...	2010	19667370
gene diversity of cyp153a and alkb alkane hydroxylases in oil-degrading bacteria isolated from the atlantic ocean.	alkane hydroxylases, including the integral-membrane non-haem iron monooxygenase (alkb) and cytochrome p450 cyp153 family, are key enzymes in bacterial alkane oxidation. although both genes have been detected in a number of bacteria and environments, knowledge about the diversity of these genes in marine alkane-degrading bacteria is still limited, especially in pelagic areas. in this report, 177 bacterial isolates, comprising 43 genera, were obtained from 18 oil-degrading consortia enriched from ...	2010	20148932
reclassification of leifsonia aurea to the genus rhodoglobus as rhodoglobus aureus comb. nov., and emended description of rhodoglobus vestalii sheridan et al. 2003.		2010	20339220
weathering-associated bacteria from the damma glacier forefield: physiological capabilities and impact on granite dissolution.	several bacterial strains isolated from granitic rock material in front of the damma glacier (central swiss alps) were shown (i) to grow in the presence of granite powder and a glucose-nh(4)cl minimal medium without additional macro- or micronutrients and (ii) to produce weathering-associated agents. in particular, four bacterial isolates (one isolate each of arthrobacter sp., janthinobacterium sp., leifsonia sp., and polaromonas sp.) were weathering associated. in comparison to what was observe ...	2010	20525872
microorganisms associated with maari, a baobab seed fermented product.	a microbiological study was carried out on baobab fermented seeds (maari) obtained from 4 different production sites in burkina faso (mansila, toulfé, ouagadougou and gorgadji). a total of 390 representative isolates comprising 251 aerobic mesophilic bacteria (amb) and 139 lactic acid bacteria (lab) were isolated and identified to species level using a combination of pheno- and genotypic methods including conventional morphological analysis, carbohydrate fermentation profiling, rep-pcr ((gtg)(5) ...	2010	20688411
leifsonia psychrotolerans sp. nov., a novel psychrotolerant species of the family microbacteriaceae from livingston island, antarctica.	a novel cold-tolerant, yellow pigmented, gram-positive, motile, facultative anaerobic bacterial strain li1t was isolated from a moss-covered soil from livingston island, antarctica, near the bulgarian station st. kliment ohridski. a comparative 16s rrna gene sequence-based phylogenetic analysis placed the strain in a clade with the species leifsonia kafniensis kfc-22t, leifsonia pindariensis pon10t, and leifsonia antarctica spc-20t, with which it showed sequence similarity of 99.0, 97.9 and 97.9 ...	2010	20833887
bacterial community diversity in the brazilian atlantic forest soils.	the aim of this study was to characterize the bacterial community diversity of the brazilian atlantic forest soil by means of both cultivation and 16s rrna clone libraries. a collection of 86 representative isolates, obtained from six samples of atlantic forest soils from the national park of serra dos órgãos (parnaso), belonged to the genera arthrobacter, bacillus, burkholderia, leifsonia, paenibacillus, pseudomonas, ralstonia, serratia, and streptomyces according to the 16s rrna sequences. rep ...	2010	20886336
rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.	most plasmids replicate only within a particular genus or family.	2010	20949038
crystal structure and centromere binding of the plasmid segregation protein parb from pcxc100.	plasmid pcxc100 from the gram-positive bacterium leifsonia xyli subsp. cynodontis uses a type ib partition system that includes a centromere region, a walker-type atpase para and a centromere-binding protein parb for stable segregation. however, parb shows no detectable sequence homology to any dna-binding motif. here, we study the parb centromere interaction by structural and biochemical approaches. the crystal structure of the c-terminal dna-binding domain of parb at 1.4 å resolution reveals a ...	2010	21123191
facultative methylotrophs from the human oral cavity and methylotrophy in strains of gordonia, leifsonia, and microbacterium.	we show that bacteria with methylotrophic potential are ubiquitous in the human mouth microbiota. numerous strains of actinobacteria (brevibacterium, gordonia, leifsonia, microbacterium, micrococcus, rhodococcus) and proteobacteria (achromobacter, klebsiella, methylobacterium, pseudomonas, ralstonia) were isolated, and one strain of each of the eleven genera was studied in detail. these strains expressed enzymes associated with methylotrophic metabolism (methanol, methylamine, and formate dehydr ...	2011	21374057
asymmetric biocatalytic reduction of 3,5-bis(trifluoromethyl) acetophenone to (1r)-[3,5-bis(trifluoromethyl)phenyl] ethanol using whole cells of newly isolated leifsonia xyli hs0904.	a novel bacterial strain hs0904 was isolated from a soil sample using 3,5-bis(trifluoromethyl) acetophenone as the sole carbon source. this bacterial isolate can asymmetrically reduce 3,5-bis(trifluoromethyl) acetophenone to (1r)-[3,5-bis(trifluoromethyl)phenyl] ethanol with high enantiometric excess (ee) value. based on its morphological, physiological characteristics, biolog, 16s rdna sequence and phylogenetic analysis, strain hs0904 was identified as leifsonia xyli hs0904. to our knowledge, t ...	2011	21614678
gyrb sequence analysis and maldi-tof ms as identification tools for plant pathogenic clavibacter.	the bacterial genus clavibacter has only one species, clavibacter michiganensis, containing five subspecies. all five are plant pathogens, among which three are recognized as quarantine pests (mentioned on the eppo a2 list). prevention of their introduction and epidemic outbreaks requires a reliable and accurate identification. currently, identification of these bacteria is time consuming and often problematic, mainly because of cross-reactions with other plant-associated bacteria in immunologic ...	2011	21802235
efficient synthesis of optically pure alcohols by asymmetric hydrogen-transfer biocatalysis: application of engineered enzymes in a 2-propanol-water medium.	we describe an efficient method for producing both enantiomers of chiral alcohols by asymmetric hydrogen-transfer bioreduction of ketones in a 2-propanol (ipa)-water medium with e. coli biocatalysts expressing phenylacetaldehyde reductase (par: wild-type and mutant enzymes) from rhodococcus sp. st-10 and alcohol dehydrogenase from leifsonia sp. s749 (lsadh). we also describe the detailed properties of mutant pars, sar268, and har1, which were engineered to have high activity and productivity in ...	2011	21739266
polyphasic approach for the characterization of rhizobial symbionts effective in fixing n(2) with common bean (phaseolus vulgaris l.).	common bean (phaseolus vulgaris l.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. the aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of arachis hypogaea, mucuna pruriens, and soils from various brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. forty-five el ...	2011	22159885
[Isolation and identification of dominant microorganisms in rhizosphere of continuous cropping with peanut].	We isolated and identified dominant microorganisms from the rhizosphere of continuous cropping with peanut, to study the relationship between dominant microorganisms and peanut continuous cropping.	2011	21866710
amnibacterium kyonggiense gen. nov., sp. nov., a new member of the family microbacteriaceae.	a gram-positive, non-motile bacterium, designated ksl51201-037(t), was isolated from anyang stream, republic of korea, and was characterized using a polyphasic taxonomic approach. comparative 16s rrna gene sequence analysis showed that strain ksl51201-037(t) belonged to the family microbacteriaceae of the class actinobacteria and exhibited 96.9 % gene sequence similarity to labedella gwakjiensis ksw2-17(t), 96.0 % to leifsonia ginsengi wged11(t) and 95.9 % to microterricola viridarii kv-677(t). ...	2011	20173001
reclassification of leifsonia ginsengi (qiu et al. 2007) as herbiconiux ginsengi gen. nov., comb. nov. and description of herbiconiux solani sp. nov., an actinobacterium associated with the phyllosphere of solanum tuberosum l.	in the context of studying the effects of transgenic fructan-producing potatoes on the community structure of phyllosphere bacteria, a group of strains closely related to the species leifsonia ginsengi was isolated. phylogenetic analysis based on 16s rrna gene sequences revealed that the new isolates and l. ginsengi dsm 19088(t) formed a lineage at the genus level and this finding was supported by chemotaxonomic characterization. the peptidoglycan type of the representative isolate, k134/01(t), ...	2011	20511458