Publications

biology and pathogenicity of eimeria alabamensis christensen, 1941, an intranuclear coccidium of cattle.		1955	14361928
development of eimeria alabamensis from cattle in mammalian cell cultures.		1971	5102112
ingestion of host-cell cytoplasm by micropores in eimeria alabamensis.		1971	5133892
fine structural aspects of development of eimeria alabamensis schizonts in cell cultures.		1972	5031344
eimeria alabamensis and eimeria brasiliensis in cattle in northern queensland.		1991	1776936
excretion of eimeria oocysts in calves during their first three weeks after turn-out to pasture.	the numbers of eimeria oocysts per gram (opg) and the dry matter content of 449 faecal samples taken from 54 calves in 8 herds in south west sweden were determined during the last 2 weeks before and the first 3 weeks after the animals were turned out to pasture. while they were housed only between 0 and 580 opg were found and in 2 of the herds the numbers of oocysts remained low after turn-out. in the other 6 herds the numbers of oocysts increased after 8 to 10 days and reached a peak of between ...	1993	8266895
peripartal excretion of eimeria oocyst by cows on swedish dairy farms and the age of calves at first excretion.	faecal samples were collected 3 times a week for 6 weeks from 22 paripartal cows and for up to 15 weeks after birth from 27 calves in 3 herds, to determine the numbers of eimeria oocysts excreted and the age at which the calves first excreted oocysts. only low numbers of oocysts were excreted by the cows and no oocysts were detected in 93% of the samples. however, half the cows excreted oocysts at least once. the age at which the calves first excreted oocysts ranged from 2.5 to at least 15 weeks ...	1993	8342468
changes in the blood composition of calves during experimental and natural infections with eimeria alabamensis.	two trials were carried out to assess the changes in blood composition of calves infected either experimentally or naturally with eimeria alabamensis. in the first, 12 calves were dosed orally with 10 to 400 million sporulated oocysts and compared with three control calves. the second trial used eight calves turned out to graze a permanent pasture known to induce e alabamensis coccidiosis in calves and eight calves turned out on to a previously ungrazed pasture. in both trials the serum activiti ...	1994	7871260
experimental eimeria alabamensis infection in calves.	four groups of three conventionally reared 2-month-old bull calves were inoculated with 10 million to 400 million sporulated oocysts of eimeria alabamensis isolated from swedish calves which had diarrhoea while at pasture. their appetite, clinical condition, growth rate and the dry matter content of their faeces were compared with those of three similar but uninoculated calves. the prepatent period was 6-8 days, and the period during which large numbers of oocysts were excreted was 2-7 days. in ...	1994	8091615
eimeria alabamensis infection as a cause of diarrhoea in calves at pasture.	large numbers of oocysts of eimeria alabamensis have been found in the faeces of calves suffering from diarrhoea shortly after being turned out to pasture. to investigate the source and clinical significance of this coccidial infection, the numbers of oocysts excreted, the consistency of the faeces and the growth rates of four groups of 12 calves were compared. group i calves were kept indoors and their diet was unchanged, group ii calves were turned out onto a previously ungrazed pasture, group ...	1994	8091616
survival of oocysts of eimeria alabamensis on pastures under different climatic conditions in sweden.	the extent to which oocysts of the coccidian parasite eimeria alabamensis can survive the winter and cause clinical coccidiosis in different parts of sweden was investigated. fecal samples were collected between may and july 1993 from calves on 59 farms where calves had grazed the same pasture for at least 5 consecutive years. the farms were situated in 9 regions of sweden with different climatic conditions in the winter. on each farm, 5 samples of feces were collected from the floor of the calf ...	1995	7572462
eimeria alabamensis coccidiosis in grazing calves: control by a long-acting baquiloprim/sulphadimidine bolus.	the excretion of eimeria oocysts, the faecal dry matter and the weight gain of three groups of 12 calves, were compared during their first 20 days of grazing on a pasture known to have been contaminated with oocysts of eimeria alabamensis during the previous year. on the day of turnout (day 0) the calves in group 1 were each treated with one bolus per 200 kg bodyweight containing 1.6 g baquiloprim and 14.4 g sulphadimidine. the calves of group 2 received the same treatment on day 3, and the calv ...	1996	8856945
immunisation of calves against eimeria alabamensis coccidiosis.	twelve calves which had been immunised with a trickle dose of altogether 100,000 oocysts of eimeria alabamensis 16 days before turnout and 12 uninoculated calves were monitored during their first 20 days of grazing on a pasture naturally contaminated with oocysts of e. alabamensis. eleven of the uninoculated calves developed gruel-like diarrhoea 3-6 days after turnout and excreted more than 850,000 oocysts/g of faeces (opg) a few days later. in contrast, none of the immunised calves developed cl ...	1996	8856947
characterization of a swedish bovine isolate of neospora caninum.	the brain of a stillborn calf, seropositive to neospora caninum and born to a seropositive cow, was homogenized and cultured on vero cells, where growth of neospora-like tachyzoites was detected after 8 weeks. the ultrastructural features of the new isolate (nc-sweb1) corresponded to those of previously published neospora isolates. in indirect immunofluorescence tests, antigens on nc-sweb1 tachyzoites were recognized by antibodies raised to a canine n. caninum isolate (nc-1) but not by antibodie ...	1997	9089715
novel elisa for detection of neospora-specific antibodies in cattle.	an enzyme-linked immunosorbent assay (elisa) to detect antibodies to neospora species in cattle was developed. whole formalin-fixed neospora caninum (nc-liverpool) tachyzoites were used as antigen and a monoclonal antibody to bovine immunoglobulin light chain and an anti-mouse horseradish peroxidase conjugate were used to reveal bound antibody. a panel of 46 sera, negative by the immunofluorescent antibody test (ifat), were used in the elisa at a serum dilution of 1:500 to calculate the negative ...	1997	9106971
the survival and transmission of oocysts of eimeria alabamensis in hay.	from a permanent pasture where young cattle had contracted coccidiosis due to eimeria alabamensis 2 years previously, hay was harvested and fed to six 2-4 month old calves 15 days, 4 months or 8 months after being harvested. the excretion of oocysts by these exposed calves was compared with that by five control calves of similar age which were housed in the same premises but fed hay from a 'coccidia-free' field. all calves fed the contaminated hay developed patent e. alabamensis infections and e ...	1997	9195731
eimeriosis in dairy cattle farms in morogoro municipality of tanzania.	coccidial oocysts were detected in 35% of 445 cattle in four medium-scale and 20 small-scale dairy farms in morogoro municipality, tanzania. the highest prevalence (56%) was observed in animals aged between 5 and 18 months, whereas lower prevalences were observed in calves (29%) aged between 12 days and 4 months and adults (30%). no coccidial oocysts were detected in calves less than 12 days old. the oocyst output was high in calves, followed by weaners; adults had the lowest oocyst output. the ...	1997	9234443
prevention of eimeria alabamensis coccidiosis by a long-acting baquiloprim/sulphadimidine bolus.	twelve calves aged 6-10 months, and 12 calves aged 10-16 months were turned out onto a permanent pasture known to have been contaminated with oocysts of eimeria alabamensis during the previous year. two days after turnout, six of the older calves and six of the younger were each treated with one bolus per 200 kg bodyweight containing 1.6 g baquiloprim and 14.4 g sulphadimidine. the other 12 calves were left untreated. the excretion of eimeria oocysts, the faecal dry matter and the weight gain of ...	1998	9561702
excretion of eimeria alabamensis oocysts in grazing calves and young stock.	the aims of the present study were to investigate the excretion of eimeria alabamensis oocysts by young cattle during their first grazing season and during the first 16 days of their second grazing period. in trial 1, nine first-season grazing heifers were studied and found to have become infected with e. alabamensis shortly after turnout. the next grazing period they were turned out on to a permanent pasture together with two first-season grazing calves. faecal samples were collected before tur ...	2000	10763379
molecular phylogeny of besnoitia and the genetic relationships among besnoitia of cattle, wildebeest and goats.	knowledge on parasites of the genus besnoitia is sparse, which are classified in the subfamily toxoplasmatinae of the phylum apicomplexa. this arrangement hypotheses that besnoitia represents the sister group to species such as toxoplasma gondii and hammondia hammondi. in order to test this hypothesis, phylogenetic analyses of 18s ribosomal dna (rdna) from besnoitia, hammondia, isospora, frenkelia, eimeria, neospora, sarcocystis and toxoplasma were performed. the 18s rdna of besnoitia besnoiti, ...	2000	11212893
a field survey on the status of internal parasites in calves on organic dairy farms in southwestern sweden.	infections with internal parasites are one of the most important causes of reduced productivity in first-grazing season cattle (fgsc). in conventional herds, nematode infections can be controlled by prophylactic anthelmintic treatments, but this is prohibited in organic production. the purpose of this investigation was to monitor the status of internal parasitism on 15 organic cattle enterprises in southwestern sweden during the 1997 and 1998 grazing seasons, and to estimate the benefits of some ...	2001	11470179
[coccidiosis causes diarrhea in calves in the pasture. pasture coccidiosis caused by eimeria alabamensis].	a severe outbreak of diarrhoea in young (age 18-20 month) cattle 14 days after their first turnout is described. in the manure large numbers eimeria alabamensis oocysts were found. this infection is considered as a case of coccidial diarrhoea in (older) calves at pasture.	2004	15032038
efficacy of toltrazuril as a metaphylactic and therapeutic treatment of coccidiosis in first-year grazing calves.	a multicentric, placebo-controlled, randomised, blinded and blocked field study was conducted to evaluate the efficacy and safety of toltrazuril (baycox, bayer ag, leverkusen, germany) in the treatment of coccidiosis in first-year grazing calves naturally infected with eimeria spp. three-hundred and thirty-one calves were enrolled in the study and allocated to one of two treatments at a ratio of 1:1. one hundred and sixty-seven animals were treated once orally with 15 mg/kg toltrazuril, and 164 ...	2005	16228269
eimeriosis in cattle: current understanding.	this report addresses various aspects of the protozoan parasite eimeria which contribute to their increasing recognition as important protozoal pathogens in cattle. among others, questions of parasite biology and epidemiology, its impact on host physiology, and control strategies are dealt with. the tenacious oocysts are found ubiquitously in the environment making an infection of calves and young cattle, the most susceptible age group, almost inevitable. further development, comprising of asexu ...	2005	16364016
clinical and epidemiological characteristics of eimeria infections in first-year grazing cattle.	infections with eimeria parasites can lead to severe diarrhoea with considerable clinical and economic consequences in first-year grazing stock. to identify and characterise the cause of diarrhoea observed during previous years, 164 animals on 14 dairy farms in northwestern germany were included in this study. the calves were physically and parasitologically examined prior to turnout and until 21 days post turnout (d.p.t.). mean animal weights decreased from 194.9 kg at the start to 189.3 kg bod ...	2006	16387444
effects of single or concurrent infections with eimeria alabamensis and gastrointestinal nematodes on the performance of calves on pasture.	twenty-four calves unexposed to pasture were allocated to four groups and inoculated with either two doses of 5 million eimeria alabamensis oocysts at turn-out (e), 90,000 l3 of ostertagia ostertagi and cooperia oncophora divided on six occasions (n) or both oocysts and larvae as above (e + n). a control group was left uninoculated (c). for 10 weeks, the groups grazed in separate uniform paddocks not previously grazed by cattle. by day 5, most calves in groups e and e + n developed clinical cocc ...	2006	16496174
a 3-year field evaluation of pasture rotation and supplementary feeding to control parasite infection in first-season grazing cattle--effects on animal performance.	to evaluate non-chemical strategies to control pasture-borne parasites in first-season grazing (fsg) cattle, a 3-year grazing trial was conducted during 2002-2004 on naturally infected pastures on a commercial beef cattle farm in sweden. a uniform pasture was divided in 4 equal 2 ha paddocks onto each of which 10, 5-9 months old dairy breed steer calves were allocated at turn-out in may each year. two strategies were evaluated: (1) turn-out onto pasture which had been grazed the previous year by ...	2006	16971047
cytokine gene expression and nf-kappab activation following infection of intestinal epithelial cells with eimeria bovis or eimeria alabamensis in vitro.	in cattle, eimeria produces clinical disease with different degrees of severity, depending on the dominant species. eimeria bovis triggers severe intestinal damage, while e. alabamensis causes minimal damage. cytokines and other factors derived from epithelial cells play important roles in inflammatory and immune responses in intestinal tissue. this study aimed to obtain a detailed view of ifn-gamma and il-4 mrna expression as well as of activation of nf-kappab p50 and p65 subunits induced by e. ...	2008	18251972
intranuclear coccidiosis in a calf.	a case of intranuclear coccidiosis in an 11-month-old female japanese black calf is described. because the calf exhibited diarrhea and failure to grow and then became moribund, euthanasia was performed. histologically, the small intestinal villi were highly atrophic, and the epithelial cells frequently had meronts or merozoites within the nuclei. a few intranuclear gametocytes were also seen. the presence of eimeria alabamensis-specific dna in the lesions was demonstrated by polymerase chain rea ...	2009	19721369
performance of second-season grazing cattle following different levels of parasite control in their first grazing season.	a 3-year grazing trial was performed during 2003-2005 on a commercial steer-producing farm in sweden to study performance of second-season grazing (ssg) cattle following different levels of parasite exposure during their first grazing season. initially, groups of 10 first-season grazing (fsg) cattle were each year assigned to four parasite control strategies: (1) turn-out onto pasture that during the previous year was grazed by ssg cattle, followed by a mid-july move to aftermath, (2) supplement ...	2010	20943321
epidemiology of eimeria and associated risk factors in cattle of district toba tek singh, pakistan.	a cross-sectional study was carried out to determine the prevalence, species characterization, and associated risk factors with eimeria of cattle of district toba tek singh from april, 2009 to march, 2010. of the total 584 fecal samples examined for eimeria, 275 (47.09%) were found infected with six species of eimeria. among the identified species of eimeria, eimeria bovis was found to be the highest prevalent species (52.36%), followed in order by eimeria zuernii, eimeria canadensis, eimeria el ...	2010	21110042
the annual cycle of shedding eimeria oocysts by european bison (bison bonasus) in the bialowieza primeval forest, poland.	abstract between april 2008, and march 2009, we analyzed the pattern of coccidian oocysts present in the feces of the european bison (bison bonasus l., 1758) and found 4 species (eimeria bovis, e. canadensis, e. ellipsoidalis, e. zuernii) previously reported from this host and 3 species (eimeria alabamensis, e. cylindrica, e. pellita), which are new host and locality records. all the species occurred in bison females and only 4 in males; e. bovis was the most prevalent in both sexes. the overall ...	2011	21506851
prevalence of coccidial infection in dairy cattle in shanghai, china.	the prevalence of coccidial infections in dairy cattle was examined in shanghai from november 2010 to march 2011. in total, 626 fecal samples from 24 dairy farms were examined; oocysts were identified to the species level based on morphological features. all herds were infected with eimeria species. the overall prevalence of coccidia was 47.1%, with the highest prevalence in <4-mo-old calves (51.8%) and the lowest in >12-mo-old cattle (27.0%). the number of oocysts per gram of feces was signific ...	2012	22590990
determination of phylogenetic relationships among eimeria species, which parasitize cattle, on the basis of nuclear 18s rdna sequence.	we analyzed almost complete 18s rdna sequences of 10 bovine eimeria species, namely eimeria alabamensis, e. auburnensis, e. bovis, e. bukidnonensis, e. canadensis, e. cylindrica, e. ellipsoidalis, e. subspherica, e. wyomingensis and e. zuernii. although these sequences showed intraspecific variation in 8 species, the sequences of each species were clustered in monophyletic groups in all species, except e. auburnensis. the sequences constituted 3 distinct clusters in a phylogenetic tree with rela ...	2013	23803494
persistence of eimeria bovis in soil.	eimeriosis is a disease that occurs globally and often affects cattle grazing on pastures contaminated with oocysts of the pathogenic species eimeria bovis, eimeria zuernii or eimeria alabamensis, respectively. nonetheless, little is understood regarding oocyst persistence on the pasture. the study was performed in the temperate climate zone. soil samples were spiked with 100,000 e. bovis oocysts in july 2010 or with 50,000 oocysts in october 2010, respectively, both either with our without addi ...	2013	23563902
intranuclear coccidiosis caused by cyclospora spp. in calves.	intranuclear coccidiosis in 3 calves is described. two calves with no genetic relationship were kept in the same pen, and the third calf was on another farm. the animals suffered from watery diarrhea or severe emaciation. histologic examination showed epithelial desquamation (cases 1 and 2) or atrophy (case 3) of the jejunal villi. coccidial meronts or merozoites were present in the nuclei of the majority of villus epithelial cells. there were rare intranuclear macrogametocytes, macrogametes, mi ...	2014	25012083
coccidia (apicomplexa: eimeriidae) of the lowland european bison bison bonasus bonasus (l.).	coprological studies conducted between 2007 and 2011 in free-roaming and captive european bison bison bonasus (linnaeus, 1758) from poland revealed 11 species of eimeria infecting the host, i.e., eimeria alabamensis, eimeria auburnensis, eimeria bovis, eimeria brasiliensis, eimeria bukidnonensis, eimeria canadensis, eimeria cylindrica, eimeria ellipsoidalis, eimeria pellita, eimeria subspherica, and eimeria zuernii. the typical host for all isolated species is cattle. the most prevalent species ...	2014	24702772
seasonal recovery of eimeria oocysts from soil on naturally contaminated pastures.	though eimeria is an important parasite of cattle, research is lacking on how the parasite persist in the pasture soils. in this study, feces samples were collected from three pastures in june and october 2010 and soil samples in april 2011. coordinates of sampling locations were recorded with global positioning system together with information about grass cover, shade, and elevation. all soil samples were collected from the same locations as the fecal samples and used in model evaluating the po ...	2014	24337512
diversity of eimeria spp. in dairy cattle of guwahati, assam, india.	to determine the prevalence and diversity of eimeria spp. in dairy cattle present in and around guwahati, kamrup district, assam, india.	2015	27047181
pathogenic characteristics of a novel intranuclear coccidia in japanese black calves and its genetic identification as eimeria subspherica.	bovine intranuclear coccidiosis is caused by the protozoans eimeria alabamensis and cyclospora spp. here, we characterized the disease and genetically identified the causative species in japanese black calves with chronic and refractory watery diarrhea. histologic examinations revealed atrophy of the jejunal villi and numerous parasites in the nucleus of epithelial cells in the jejunum. based on molecular analyses using 18s ribosomal rna gene-specific primers that we designed, the parasites were ...	2017	28983698