Publications

head transcriptomes of two closely related species of fruit flies of the anastrepha fraterculus group reveals divergent genes in species with extensive gene flow.	several fruit flies species of the anastrepha fraterculus group are of great economic importance for the damage they cause to a variety of fleshy fruits. some species in this group have diverged recently, with evidence of introgression, showing similar morphological attributes that render their identification difficult, reinforcing the relevance of identifying new molecular markers that may differentiate species. we investigated genes expressed in head tissues from two closely related species: a ...	0	27558666
oviposition deterring pheromone inanastrepha fraterculus flies.	anastrepha fraterculus (wiedemann) females were found to deposit a water-soluble, durable, oviposition-deterring pheromone during ovipositor dragging on fruit after egg-laying. we present evidence that the occurrence of pheromone deposition after egg-laying, the amount deposited, and departure from the fruit without additional egg-laying after pheromone deposition are flexible traits ina. fraterculus, varying in expression according to fruit size and other factors. unlikerhagoletis, a. fratercul ...	1982	24415123
genetical and biochemical comparisons of alcohol dehydrogenase isozymes from anastrepha fraterculus and a. obliqua (diptera: tephritidae): evidence for gene duplication.	the electrophoretic patterns of the enzyme alcohol dehydrogenase (adh) from anastrepha fraterculus and a. obliqua were studied. two loci were found to code for the enzyme in a. fraterculus, and three in a. obliqua. in both species, all isozymes were active in third-instar larvae. a cationic isozyme (adh-1) was active mainly in the visceral fat body of both species. in a. fraterculus, the locus had an anionic polymorphic isozyme (adh-3) that was detected in the parietal fat body. in addition to t ...	1986	2938573
digestion of bacteria and the role of midgut lysozyme in some insect larvae.	1. lysozyme is absent from tissues other than the midgut in the drug-feeding larvae of musca domestica (diptera, cyclorrhapha, muscidae) and in the fruit-feeding larvae of anastrepha fraterculus (diptera, cyclorrhapha, tephritidae), whereas in the detritus-feeding larvae of trichosia pubescens (diptera, nematocera, sciaridae) lysozyme is only found in the hemolymph and in the fat body. 2. a. fraterculus larvae have a midgut region with a luminal ph of 3.4, and display a pepstatin-inhibited acid ...	1991	1799969
discrimination between the immature stages of ceratitis capitata and anastrepha fraterculus (diptera:tephritidae) populations by random amplified polymorphic dna polymerase chain reaction.	a random amplified polymorphic dna polymerase chain reaction method using 10-base long commercial random primers was used to identify polymorphisms in argentine populations of 2 fruit fly pests. a fast and reliable discrimination between mediterranean fruit fly, ceratitis capitata (wiedemann), and anastrepha fraterculus (wiedemann) immature or mature stages was obtained using as few as 1 insect per assay. the population of origin of immature individuals can be identified unambiguously using 2 pr ...	1996	8913115
the effects of constant and alternating temperatures on the reproductive potential, life span, and life expectancy of anastrepha fraterculus (wiedemann) (diptera: tephritidae).	ovarian development, oviposition, larval eclosion, ornithine decarboxylase (odc) activity, ovarian, testis and ejaculatory apodeme measurements (length, width, and area), and the number of spermatozoa of anastrepha fraterculus (wiedemann) were analyzed at alternating (20 degrees/6 degrees c and 20 degrees/13 degrees c) and constant (6 degrees c; 25 degrees c) temperatures. life span and life expectancy were also analyzed for both genders. all the results suggest that temperature, especially alte ...	2002	12659028
karyotypic and molecular identification of laboratory stocks of the south american fruit fly anastrepha fraterculus (wied) (diptera: tephritidae).	the taxonomic status of the tephritid pest anastrepha fraterculus (wied.) is a controversial subject, mainly because of misinterpretation of the observed genetic variation. in this work, the different karyotypes and dna polymorphism of a geographically defined population from northeastern argentina were studied, using derived stocks maintained in the laboratory during 25 generations. the karyotypes were analyzed using c-banding and n-banding techniques, while dna analysis was performed through t ...	2003	14503596
nonhost status of citrus sinensis cultivar valencia and c. paradisi cultivar ruby red to mexican anastrepha fraterculus (diptera: tephritidae).	anastrepha fraterculus (wiedemann) is recognized as a pest of citrus, apples, and blackberries in south america. in mexico, it is mainly found in fruit of the family myrtaceae and has never been reported infesting citrus. here, we sought to determine whether females stemming from mexican a. fraterculus populations (collected in the state of veracruz) would lay eggs in 'valencia' oranges and 'ruby red' grapefruit and, if so, whether larvae would hatch and develop. we worked under laboratory and s ...	2003	14977105
native and introduced host plants of anastrepha fraterculus and ceratitis capitata (diptera: tephritidae) in northwestern argentina.	wild or commercially grown, native and exotic fruit were collected in 30 localities in the tucumán province (nw argentina) from january 1990 to december 1995 to determine their status as hosts of anastrepha fraterculus (wiedemann) and/or ceratitis capitata (wiedemann), the only two fruit fly species of economic and quarantine importance in argentina. a total of 84,094 fruit (3,466.1 kg) representing 33 species (7 native and 26 exotic) in 15 plant families were sampled. we determined the followin ...	2003	14503581
morphometric analysis of mexican and south american populations of the anastrepha fraterculus complex (diptera: tephritidae) and recognition of a distinct mexican morphotype.	discriminant function and cluster analyses were performed on 19 morphometric variables of the aculeus, wing and mesonotum to determine whether populations of anastrepha fraterculus (wiedemann) from different parts of mexico could be distinguished from populations from south america. samples were collected from seven localities across mexico, two from brazil, and one each from colombia and argentina. results showed there were statistically significant differences between mexican and south america ...	2004	15541188
ornithine decarboxylase activity during the development of anastrepha fraterculus (diptera, tephritidae).	ornithine decarboxylase (odc) (ec 4.1.1.17) is very important for polyamine biosynthesis, which is required for main biological events. in the present study, odc activity was measured in samples of anastrepha fraterculus's egg, larva, pupa body and abdomen, adult body, ovaries, and fat body of young females, and in ovaries of mature flies. the kinetic parameters (km app and vmax) for odc activity were determined for pupa, larva, and young ovary. odc activity showed fluctuations during a. fraterc ...	2004	15540277
changes in ornithine decarboxylase activity in response to temperature stress and stimulation of juvenile hormone in anastrepha fraterculus (diptera, tephritidae).	ornithine decarboxylase (odc) activity was analyzed in anastrepha fraterculus (diptera) females (4 days old) submitted to temperature stress (6 degrees c and 20/6 degrees c) and the topical application of juvenile hormone (jh). odc activity and ejaculatory apodeme measurements (length and width) were made in males (15 days old) after 6 degrees c stress. jh dose of 500 ng and incubation of 3, 7, and 18 h increased odc activity. females reared at 6 degrees c and 20/6 degrees c had higher odc activ ...	2006	16933279
diversity of flies (diptera: tephritidae and lonchaeidae) in organic citrus orchards in the vale do rio caí, rio grande do sul, southern brazil.	this study was carried out in the counties of montenegro and pareci novo located in the region of the vale do rio cai, rio grande do sul, southern brazil, aiming to determine the fruit fly species of tephritidae and lonchaeidae that occur in organic orchards of sweet orange [citrus sinensis (l.) osb.] cultivar céu, and murcott tangor (citrus reticulata blanco x c. sinensis), during the fruit ripening stages in 2003 and 2004. eight mcphail traps baited with integral grape juice diluted to 25% wer ...	2006	17144140
long aculeus and behavior of anastrepha ludens render gibberellic acid ineffective as an agent to reduce 'ruby red' grapefruit susceptibility to the attack of this pestiferous fruit fly in commercial groves.	treating mexican grapefruit with gibberellic acid (ga3) before color break, significantly delayed peel color change and increased peel puncture resistance, but it did not reduce grapefruit susceptibility to mexican fruit fly, anastrepha ludens (loew) attack under natural conditions. despite ga3 treatments, larval infestation levels increased with higher fruit fly populations, which also increased as the season progressed. late in the season, infestation levels were even higher in ga3-treated fru ...	2006	16937671
entomopathogenicity of native bacteria from anastrepha fraterculus and ceratitis capitata against the pest phyllocnistis citrella.	in piura (peru), the pest phyllocnistis citrella stainton damages the photosynthetic rate and new bud production of citrus aurantiifolia swingle (sweet lemon), decreasing the yield, productivity and commercial price of its fruit. biological control was evaluated through the crossed effect of bacteria obtained from pests (anastrepha fraterculus wied., ceratitis capitata wied. and rhynchophorus palmarum l.) that are pathogenic against their original host species. enterobacter cloacae (jordan) horm ...	2007	17348070
[susceptibility of six arabic coffee cultivars to fruit flies (diptera: tephritoidea) under shaded and unshaded organic management in valença, state of rio de janeiro, brazil].	the infestation indices by fruit flies were determined for six cultivars of coffea arabica l. in shaded and unshaded systems under organic management. the experiment was set in a completely randomized design with a split-split-plot arrangement and four replicates. a 250g-sample of maturing fruits per plot was harvested in may 2005. the cultivars icatu amarelo and catucaí amarelo were the least susceptible to attack by tephritids in both systems. as for lonchaeids, oeiras, catucaí amarelo and cat ...	2007	17607461
intermediate metabolism during the ontogenetic development of anastrepha fraterculus (diptera: tephritidae).	the fruit fly anastrepha fraterculus is a major pest of native and exotic fruit trees in south america. changes in weight, water content and metabolism were observed during its ontogenetic development in standard conditions (25 degrees c, rh=60% and 14 h:10 h photoperiod). the metabolic variables glycogen, total proteins, triglycerides and total lipids were measured by means of spectrophotometric methods. the results were correlated with pupae metamorphosis, temporal pattern, and beginning of ad ...	2007	17020813
phylogeographic studies on natural populations of the south american fruit fly, anastrepha fraterculus (diptera: tephritidae).	anastrepha fraterculus is an important pest of commercial fruits in south america. the variability observed for morphological and behavioural traits as well as genetic markers suggests that a. fraterculus represents a complex of synmorphic species rather than a single biological species. we studied the correlation between geographical distribution and genetic variation in natural populations from argentina and south brazil. fragments of the mitochondrial gene coii were sequenced in 28 individual ...	2008	17318315
[faunistic analysis of fruit flies (diptera: tephritidae) in the northern and northwestern regions of rio de janeiro state, brazil].	this study aimed to characterize the fruit fly populations in three municipalities of the northern region and two municipalities of the northwestern region of rio de janeiro state, brazil, and to evaluate the similarity among their populations. a faunistic analysis was performed from the fruit fly specimens captured in plastic mcphail traps with an aqueous solution of hydrolyzed protein to 5% placed in orchards of guava (psidium guajava l.) and/or other fruits during 26 months. the total of 3,95 ...	2008	18368244
[measurement of the aculeus for the characterization of five anastrepha species of the fraterculus group (diptera: tephritidae)].	species identification of the genus anastrepha schiner is based mostly on the shape of the aculeus apex. in some species groups, such as fraterculus, species are separated by subtle differences in the aculeus apex, namely anastrepha fraterculus (wied.), a. obliqua (macquart), a. sororcula zucchi, a. zenildae zucchi and a. turpiniae stone. in order to help the identification, the aculei of these five species from 25 localities of 17 brazilian states were measured. the aculeus and apex lengths of ...	2008	18575692
effect ofalpha-difluormethylornithine on anastrepha fraterculus (diptera, tephritidae) ovary size.	ovarian sizes (length and width) were measured in young females of anastrepha fraterculus (wiedemann) (diptera, tephritidae) subjected or not to the inhibitor alpha-difluormethylornithine (alpha-dfmo). the most effective concentration of alpha-dmfo used was 50 mm and the ovarian measurements (length and width) of the treated females were smaller than those of females not treated with alpha-dmfo. these data may suggest some relationship between ornithine decarboxylase (odc) and sexual maturation ...	2009	19347158
[parasitoids (hymenoptera: braconidae) of fruit flies (diptera: tephritidae) in the state of são paulo, brazil: associated plants and parasitism].	a total of 3,008 braconid parasitoids in 26 species of fruit fly host plants were collected in 33 localities of são paulo state. the majority of the braconids (96.2%) belonged to four opiinae species, doryctobracon areolatus (szépligeti), doryctobracon brasiliensis (szépligeti), opius bellus (gahan) and utetes anastrephae (viereck), and the remaining 3.8%, a single species of alysiinae, asobara anastrephae (muesebeck). doryctobracon areolatus was collected on anastrepha fraterculus (wied), a. ob ...	2009	19618046
efficacy of indigenous entomopathogenic nematodes (rhabditida: heterorhabditidae, steinernematidae), from rio grande do sul brazil, against anastrephafraterculus (wied.) (diptera: tephritidae) in peach orchards.	laboratory, greenhouse, and field experiments were performed with the objective of selecting efficient indigenous strains of entomopathogenic nematodes (epns) from rio grande do sul (rs) state, brazil, for controlling the south american fruit fly, anastrepha fraterculus (wied.). laboratory experiments were conducted in 24 well-plates filled with sterile sand and one insect per well. in greenhouse experiments, plastic trays filled with soil collected from the field were used, while in field exper ...	2009	19460384
a new generation of x ray irradiators for insect sterilization.	recent fears of terrorism have provoked an increase in delays and denials of transboundary shipments of radioisotopes. this represents a serious constraint to sterile insect technique (sit) programs around the world as they rely on the use of ionizing radiation from radioisotopes for insect sterilization. to validate a novel x ray irradiator, a series of studies on ceratitis capitata (wiedemann) and anastrepha fraterculus (wiedemann) (diptera: tephritidae) were carried out, comparing the relativ ...	2010	20214372
diversity of anastrepha spp. (diptera: tephritidae) and associated braconid parasitoids from native and exotic hosts in southeastern bahia, brazil.	we documented fruit fly-host associations and infestation rates over 5 yr in the state of bahia, brazil, by systematically collecting native and introduced fruits in backyard and commercial orchards, experimental stations, and patches of native vegetation. fruit were collected in multiple sites in the southern and southernmost regions of bahia. a total of 942.22 kg from 27 fruit species in 15 plant families was collected throughout this study. of these, 15 plant species from six families were in ...	2010	22546440
Anastrepha ludens and Anastrepha serpentina (Diptera: Tephritidae) do not infest Psidium guajava (Myrtaceae), but Anastrepha obliqua occasionally shares this resource with Anastrepha striata in nature.	This study examined whether economically important fruit fly species Anastrepha ludens (Loew), Anastrepha serpentina (Wiedemann), and Anastrepha obliqua (Macquart) (Diptera: Tephritidae) may opportunistically exploit guavas, Psidium guajava L. (Myrtaceae), growing near preferred natural hosts. We collected 3,459 kg of guavas and 895 kg of other known host species [sour orange, Citrus aurantium L.; grapefruit, Citrus paradisi Macfadyen; mango, Mangifera indica L.; white sapote, Casimiroa edulis L ...	2011	21882684
histopathological events and detection of metarhizium anisopliae using specific primers in infected immature stages of the fruit fly anastrepha fraterculus (wiedemann, 1830) (diptera: tephritidae).	the fungus metarhizium anisopliae is used on a large scale in brazil as a microbial control agent against the sugar cane spittlebugs, mahanarva posticata and m. fimbriolata (hemiptera., cercopidae). we applied strain e9 of m. anisopliae in a bioassay on soil, with field doses of conidia to determine if it can cause infection, disease and mortality in immature stages of anastrepha fraterculus, the south american fruit fly. all the events were studied histologically and at the molecular level duri ...	2011	21437404
effects of male nutrition on sperm storage and remating behavior in wild and laboratory anastrepha fraterculus (diptera: tephritidae) females.	male physiological condition can affect his ability to modulate female sexual receptivity. thus, studying this aspect can have biological and practical implications. here, we examine how male nutritional status affected the amount of sperm stored, remating rate and refractory period of the tephritid fruit fly anastrepha fraterculus (wiedemann) females. both wild and laboratory flies were evaluated. we also examine female sperm storage patterns. experiments were carried out by manipulating male a ...	2011	21864536
male courtship behavior of the south american fruit fly, anastrepha fraterculus, from an argentinean laboratory strain.	the south american fruit fly anastrepha fraterculus (wiedemann) (diptera: tephritidae) is a pest of fruit species of warm regions of the americas, including argentina. some authors claim that this taxon includes a group of cryptic species. in order to evaluate possible targets of sexual selection, it is necessary to analyze ethological aspects of male courtship and identify particular steps that strongly influence mating success. a mating test designed to evaluate behavioral differences between ...	2011	22958000
Management of protein intake in the fruit fly Anastrepha fraterculus.	This work tested if carbohydrates and proteins ingestion is regulated in the South American fruit fly, Anastrepha fraterculus, to optimize survival and reproduction. Adult food treatments were established by providing sugar and hydrolyzed yeast in various combinations either alone or mixed at a standard 3:1 ratio (sugar:hydrolyzed yeast). Individual food consumption was assessed and related to survival patterns. The effects of adult feeding on fecundity and fertility patterns were investigated i ...	2011	21896276
morphometry and distribution of sensilla on the antennae of anastrepha fraterculus (wiedemann) (diptera: tephritidae).	antennal sensilla of anastrepha fraterculus (wied.) were examined using scanning electron microscopy. in the flagellum, there are trichoid, basiconic, clavate type i and ii, and styloconic sensilla and microtrichia. only microtrichiae and chaetica sensilla were observed in the scape and pedicel. the number of sensilla in the flagellum was similar between sexes. at the apex there was a higher density of trichoid and an absence of clavate sensilla, while basiconic sensilla were more abundant in th ...	2011	21584402
positive and purifying selection influence the evolution of doublesex in the anastrepha fraterculus species group.	the gene doublesex (dsx) is considered to be under strong selective constraint along its evolutionary history because of its central role in somatic sex differentiation in insects. however, previous studies of dsx used global estimates of evolutionary rates to investigate its molecular evolution, which potentially miss signals of adaptive changes in generally conserved genes. in this work, we investigated the molecular evolution of dsx in the anastrepha fraterculus species group (diptera, tephri ...	2012	22428050
cuticular hydrocarbons of the south american fruit fly anastrepha fraterculus: variability with sex and age.	insect cuticular hydrocarbons are usually species-specific mixtures and may serve for species and gender recognition. they are, therefore, widely used in the chemotaxonomy and zoogeography of various insect taxa. in order to provide a basic study for further comparative analyses of cuticular hydrocarbon (chc) profiles of cryptic species hidden within the south american fruit fly anastrepha fraterculus complex (diptera: tephritidae), we analyzed the composition of the chcs and their production wi ...	2012	22948785
random mating and reproductive compatibility among argentinean and southern brazilian populations of anastrepha fraterculus (diptera: tephritidae).	as a prerequisite for area-wide application of the sterile insect technique in an area encompassing northern argentina and southern brazil, prezygotic and postzygotic reproductive compatibility among three geographically distant populations in the area was tested. in field cages, sexually mature adults of each population were found to be sexually compatible, mating duration was not affected by fly origin and there was no clear evidence of spatial partition of mating location. in the laboratory, ...	2012	22360877
biological control of tephritid fruit flies in argentina: historical review, current status, and future trends for developing a parasitoid mass-release program.	in argentina there are two tephritid fruit fly species of major economic and quarantine importance: the exotic ceratitis capitata that originated from southeast africa and the native anastrepha fraterculus. in recent years, the use of fruit fly parasitoids as biocontrol agents has received renewed attention. this increasing interest has recently led to the establishment of a program for the mass rearing of five million diachasmimorpha longicaudata parasitoids per week in the bioplanta san juan f ...	2012	26466633
factors affecting anastrepha fraterculus female receptivity modulation by accessory gland products.	in the context of the sterile insect technique (sit), mass-rearing and male irradiation are imperative. post-teneral treatments such as the addition of protein in adult's male diet and male hormonal treatment are used to improve sexual performance and to accelerate sexual maturation. in this work we investigated the effect of male accessory glands products (agps) on female receptivity of the south american fruit fly anastrepha fraterculus (wiedemann), and the effect of strain rearing history, ma ...	2012	21907717
species of anastrepha (diptera: tephritidae) captured in a guava orchard (psidium guajava l., myrtaceae) in boa vista, roraima, brazil.	the guava fruit (psidium guajava) is among the most strongly affected by fruit flies in brazil. in the brazilian amazon, 11 species of anastrepha have been reported in guava orchards to date. this work aimed to identify the species of anastrepha present in a guava orchard in the municipality of boa vista, determine the species infesting the fruits, and identify any parasitoids present. two mcphail traps with food bait were installed and weekly collections were made between january and december 2 ...	2013	24789406
neem derivatives are not effective as toxic bait for tephritid fruit flies.	neem derivatives have been widely touted as replacements for pesticides. a feasible replacement of synthetic insecticides in the management of fruit flies could be to use neem products in baits. this study evaluated the bioactivity of neem (azadirachta indica a. juss) derivatives in bait for adults of anastrepha fraterculus (wiedemann) and ceratitis capitata (wiedemann). the estimated lcs50 values for a. fraterculus and c. capitata were 7,522 ppm (18.40 ppm of azadirachtin) and 1,368 ppm (3.35 p ...	2013	24020292
precocious sexual signalling and mating in anastrepha fraterculus (diptera: tephritidae) sterile males achieved through juvenile hormone treatment and protein supplements.	sexual maturation of anastrepha fraterculus is a long process. methoprene (a mimic of juvenile hormone) considerably reduces the time for sexual maturation in males. however, in other anastrepha species, this effect depends on protein intake at the adult stage. here, we evaluated the mating competitiveness of sterile laboratory males and females that were treated with methoprene (either the pupal or adult stage) and were kept under different regimes of adult food, which varied in the protein sou ...	2013	22929968
the identity of specimens of the anastrepha fraterculus complex (diptera, tephritidae) with atypical aculeus tip.	several specimens collected in paraguay along with anastrepha fraterculus (sensu lato) have an aculeus tip similar to species from the fraterculus complex, but the teeth of the aculeus of these specimens are poorly defined. as anastrepha species identification is based mostly on subtle differences in the aculeus tip, we studied these specimens with atypical aculeus tips (with poorly defined teeth) that slightly differs from the aculeus tip of species of the fraterculus complex (with well-develop ...	2013	27193280
effect of host plant chemistry on genetic differentiation and reduction of gene flow among anastrepha fraterculus (diptera: tephritidae) populations exploiting sympatric, synchronic hosts.	herbivore host specialization includes changes in behavior, driven by locally induced adaptations to specific plants. these adaptations often result in sexual isolation that can be gauged through detection of reduced gene flow between host associated populations. hypothetically, reduced gene flow can be mediated both by differential response to specific plant kairomones and by the influence of larval diet on some adult traits such as pheromone composition. these hypotheses could serve as a model ...	2013	23905743
remating behavior in anastrepha fraterculus (diptera: tephritidae) females is affected by male juvenile hormone analog treatment but not by male sterilization.	the sterile insect technique (sit) has been proposed as an area-wide method to control the south american fruit fly, anastrepha fraterculus (wiedemann). this technique requires sterilization, a procedure that affects, along with other factors, the ability of males to modulate female sexual receptivity after copulation. numerous pre-release treatments have been proposed to counteract the detrimental effects of irradiation, rearing and handling and increase sit effectiveness. these include treatin ...	2013	23340454
transcriptome analysis of female reproductive tissues of anastrepha obliqua and molecular evolution of eggshell proteins in the fraterculus group.	the investigation of cdna libraries has been an important tool for the identification of new genes in nonmodel species such as the fruit flies from the anastrepha fraterculus group. in the present study, we constructed a cdna library from the female reproductive tissues of anastrepha obliqua aiming to identify genes with high evolutionary rates. we sequenced 2304 clones obtained from the female reproductive tissues of a. obliqua flies. the expressed sequence tags generated a total of 816 unigene ...	2013	24137793
microsatellite markers from the 'south american fruit fly' anastrepha fraterculus: a valuable tool for population genetic analysis and sit applications.	anastrepha fraterculus wiedemann is a horticultural pest which causes significant economic losses in the fruit-producing areas of the american continent and limits the access of products to international markets. the use of environmentally friendly control strategies against this pest is constrained due to the limited knowledge of its population structure.	2014	25471285
effect of constant temperatures on the biology, life table, and thermal requirements of aganaspis pelleranoi (hymenoptera: figitidae), a parasitoid of anastrepha fraterculus (diptera: tephritidae).	aganaspis pelleranoi (brèthes, 1924) (hymenoptera: figitidae) is a koinobiont endoparasitoid of larvae of species of the genus anastrepha and of ceratitis capitata. it is a candidate for use as a biological control agent, as under field conditions, it may reach a parasitism rate of 62%. the objective of this study was to determine the effect of different constant temperatures on biological parameters of a. pelleranoi when parasitizing the larva of anastrepha fraterculus (wiedemann, 1830) (dipter ...	2014	24612939
dynamics of genetic variability in anastrepha fraterculus (diptera: tephritidae) during adaptation to laboratory rearing conditions.	anastrepha fraterculus is one of the most important fruit fly plagues in the american continent and only chemical control is applied in the field to diminish its population densities. a better understanding of the genetic variability during the introduction and adaptation of wild a. fraterculus populations to laboratory conditions is required for the development of stable and vigorous experimental colonies and mass-reared strains in support of successful sterile insect technique (sit) efforts.	2014	25471362
genetics and biology of anastrepha fraterculus: research supporting the use of the sterile insect technique (sit) to control this pest in argentina.	two species of true fruit flies (taxonomic family tephritidae) are considered pests of fruit and vegetable production in argentina: the cosmopolitan mediterranean fruit fly (ceratitis capitata wiedemann) and the new world south american fruit fly (anastrepha fraterculus wiedemann). the distribution of these two species in argentina overlaps north of the capital, buenos aires. regarding the control of these two pests, the varied geographical fruit producing regions in argentina are in different f ...	2014	25471175
differences in sperm storage and remating propensity between adult females of two morphotypes of the anastrepha fraterculus (diptera: tephritidae) cryptic species complex.	the south american fruit fly, anastrepha fraterculus, is a complex of cryptic species composed of at least seven morphotypes. some of them, such as the peruvian and brazilian 1 morphotypes (which include argentinean populations), exhibit strong pre-copulatory isolation, yet it is possible to obtain heterotypic crosses when forcing copulation of adults under laboratory conditions. the cross involving peruvian males and argentinean females produces f1 offspring with reduced viability in terms of e ...	2014	24702958
toxic effect of citrus peel constituents on anastrepha fraterculus wiedemann and ceratitis capitata wiedemann immature stages.	the toxicity of essential oils from the citrus peel has been proposed as the major resistance mechanism offered by citrus to fruit fly infestation. we evaluated the insecticidal activity of the ether extracts from the lemon (citrus limon [l.] burm.) and grapefruit (c. paradisi macfadyen) peel as well as from limonene and citral against anastrepha fraterculus (wiedemann) and ceratitis capitata (wiedemann) immature stages. we also evaluated the toxicity of the extracts at two ripening stages. extr ...	2014	25237738
viability of cabralea canjerana extracts to control the south american fruit fly, anastrepha fraterculus.	several representatives of meliaceae contain biologically active compounds that are toxic to insects with few negative effects on the environment and humans. our study evaluated the activity of ethyl acetate and ethanol extracts from the fruit and seeds of cabralea canjerana (vellozo) mart (sapindales: meliaceae) on anastrepha fraterculus (wiedemann) (diptera: tephritidae). limonoids and triterpenes were detected in fruit and seed extracts. each extract was added to an artificial diet at three c ...	2014	25373194
assessing the risk of invasion by tephritid fruit flies: intraspecific divergence matters.	widely distributed species often show strong phylogeographic structure, with lineages potentially adapted to different biotic and abiotic conditions. the success of an invasion process may thus depend on the intraspecific identity of the introduced propagules. however, pest risk analyses are usually performed without accounting for intraspecific diversity. in this study, we developed bioclimatic models using maxent and boosted regression trees approaches, to predict the potential distribution in ...	2015	26274582
current knowledge of the species complex anastrepha fraterculus (diptera, tephritidae) in brazil.	the study of the species complex anastrepha fraterculus (af complex) in brazil is especially important in a taxonomical, evolutionary and pest management context, because there are evidences that some of them may occur in sympatry. in this review, we analyzed the main results supporting evidences that three cryptic species occur in brazil. the taxonomical and phylogenetic relationships based on eggshell morphology, adult morphometrics, as well as cytotaxonomy and genetic differentiations are dis ...	2015	26798261
characterisation of the chemical profiles of brazilian and andean morphotypes belonging to the anastrepha fraterculus complex (diptera, tephritidae).	fruit fly sexual behaviour is directly influenced by chemical and non-chemical cues that play important roles in reproductive isolation. the chemical profiles of pheromones and cuticular hydrocarbons (chs) of eight fruit fly populations of the andean, brazilian-1 and brazilian-3 morphotypes of the anastrepha fraterculus cryptic species complex originating from colombia (four populations) and brazil (four populations) were analysed using two-dimensional gas chromatography with mass spectrometric ...	2015	26798260
nuclear ribosomal internal transcribed spacer 1 (its1) variation in the anastrepha fraterculus cryptic species complex (diptera, tephritidae) of the andean region.	the nuclear ribosomal internal transcribed spacer 1 (its1) was sequenced for anastrepha fraterculus (wiedemann, 1830) originating from 85 collections from the northern and central andean countries of south america including argentina (tucumán), bolivia, perú, ecuador, colombia, and venezuela. the its1 regions of additional specimens (17 collections) from central america (méxico, guatemala, costa rica, and panamá), brazil, caribbean colombia, and coastal venezuela were sequenced and together with ...	2015	26798259
evaluating mating compatibility within fruit fly cryptic species complexes and the potential role of sex pheromones in pre-mating isolation.	the study of sexual behavior and the identification of the signals involved in mate recognition between con-specifics are key components that can shed some light, as part of an integrative taxonomic approach, in delimitating species within species complexes. in the tephritidae family several species complexes have received particular attention as they include important agricultural pests such as the ceratitis fasciventris (bezzi), ceratitis anonae (graham) and ceratitis rosa karsch (far) complex ...	2015	26798257
sex chromosomes in mitotic and polytene tissues of anastrepha fraterculus (diptera, tephritidae) from argentina: a review.	cytogenetics, which is considered a fundamental tool to understand basic genetic and genomic issues of species, has greatly contributed to the description of polymorphisms both at inter- and intra-specific level. in fact, cytogenetics was one of the first approaches used to propose anastrepha fraterculus (diptera: tephritidae) as a complex of cryptic species. different morphological variants of sex chromosomes have been reported among argentinean populations of anastrepha fraterculus. however, s ...	2015	26798255
morphometric study of third-instar larvae from five morphotypes of the anastrepha fraterculus cryptic species complex (diptera, tephritidae).	the occurrence of cryptic species among economically important fruit flies strongly affects the development of management tactics for these pests. tools for studying cryptic species not only facilitate evolutionary and systematic studies, but they also provide support for fruit fly management and quarantine activities. previous studies have shown that the south american fruit fly, anastrepha fraterculus, is a complex of cryptic species, but few studies have been performed on the morphology of it ...	2015	26798253
resolving cryptic species complexes of major tephritid pests.	an fao/iaea co-ordinated research project (crp) on "resolution of cryptic species complexes of tephritid pests to overcome constraints to sit application and international trade" was conducted from 2010 to 2015. as captured in the crp title, the objective was to undertake targeted research into the systematics and diagnostics of taxonomically challenging fruit fly groups of economic importance. the scientific output was the accurate alignment of biological species with taxonomic names; which led ...	2015	26798252
development of anastrepha fraterculus (diptera: tephritidae) related to the phenology of blueberry, blackberry, strawberry guava, and surinam cherry fruits.	anastrepha fraterculus (wiedemann, 1830) is the main pest of temperate climate orcharding. the study investigated the development of a. fraterculus related to phenological stage of blueberry, blackberry, strawberry guava, and surinam cherry trees. the phenological stages i (green fruits), ii (intermediate ripening stage of fruits), and iii (fruits close to harvesting) were determined, and they are from 8th, 10th, and 11th week; 6th, 8th, and 9th week; 8th, 13th, and 16th week; and 5th, 6th, and ...	2015	26470120
relevant genetic differentiation among brazilian populations of anastrepha fraterculus (diptera, tephritidae).	we used a population genetic approach to detect the presence of genetic diversity among six populations of anastrepha fraterculus across brazil. to this aim, we used simple sequence repeat (ssr) markers, which may capture the presence of differentiative processes across the genome in distinct populations. spatial analyses of molecular variance were used to identify groups of populations that are both genetically and geographically homogeneous while also being maximally differentiated from each o ...	2015	26798258
taxonomy and phenotypic relationships of the anastrepha fraterculus complex in the mesoamerican and pacific neotropical dominions (diptera, tephritidae).	previous morphometric studies based on linear measurements of female structures of the aculeus, mesonotum, and wing revealed the existence of seven morphotypes within the anastrepha fraterculus cryptic species complex along the neotropical region. the current research followed linear and geometric morphometric approaches in 40 population samples of the nominal species anastrepha fraterculus (wiedemann) spread throughout the meso-american and pacific neotropical dominions (including mexico, centr ...	2015	26798256
correction: male sexual behavior and pheromone emission is enhanced by exposure to guava fruit volatiles in anastrepha fraterculus.		2015	26024371
male sexual behavior and pheromone emission is enhanced by exposure to guava fruit volatiles in anastrepha fraterculus.	plant chemicals can affect reproductive strategies of tephritid fruit flies by influencing sex pheromone communication and increasing male mating competitiveness.	2015	25923584
toward an automated identification of anastrepha fruit flies in the fraterculus group (diptera, tephritidae).	in this study, we assess image analysis techniques as automatic identifiers of three anastrepha species of quarantine importance, anastrepha fraterculus (wiedemann), anastrepha obliqua (macquart), and anastrepha sororcula zucchi, based on wing and aculeus images. the right wing and aculeus of 100 individuals of each species were mounted on microscope slides, and images were captured with a stereomicroscope and light microscope. for wing image analysis, we used the color descriptor local color hi ...	2016	27156899
basis for the development of a rearing technique of aganaspis pelleranoi (hymenoptera: figitidae) in anastrepha fraterculus (tephritidae: diptera).	anastrepha fraterculus (wiedemann) (diptera: tephritidae) is among the most important pests of fruit trees in south america, where it can cause losses of up to 100% in fruit orchards. the endoparasitoid aganaspis pelleranoi (brèthes) (hymenoptera: figitidae) is one of the main natural enemies of a. fraterculus in neotropical countries and can be used as a biological control agent against this pest. this study aimed to provide background biological information for the development of a method for ...	2016	27106221
co-infestation and spatial distribution of bactrocera carambolae and anastrepha spp. (diptera: tephritidae) in common guava in the eastern amazon.	field infestation and spatial distribution of introduced bactrocera carambolae drew and hancock and native species of anastrepha in common guavas [psidium guajava (l.)] were investigated in the eastern amazon. fruit sampling was carried out in the municipalities of calçoene and oiapoque in the state of amapá, brazil. the frequency distribution of larvae in fruit was fitted to the negative binomial distribution. anastrepha striata was more abundant in both sampled areas in comparison to anastreph ...	2016	27638949
morphometric differentiation among anastrepha fraterculus (diptera: tephritidae) exploiting sympatric alternate hosts.	anastrepha fraterculus (wiedemann) is currently considered a complex of cryptic species infesting fruits from mexico to argentina and represents an interesting biological model for evolutionary studies. moreover, detecting and quantifying behavioral, morphological, and genetic differentiation among populations is also relevant to the application of environment-friendly control programs. here, phenotypic differentiation among individuals coexisting in the wild in a northern region of argentina wa ...	2016	26787122
the male ejaculate as inhibitor of female remating in two tephritid flies.	the inhibition of female receptivity after copulation is usually related to the quality of the first mating. males are able to modulate female receptivity through various mechanisms. among these is the transfer of the ejaculate composed mainly by sperm and accessory gland proteins (agps). here we used the south american fruit fly anastrepha fraterculus (where agp injections inhibit female receptivity) and the mexican fruit fly anastrepha ludens (where injection of agps failed to inhibit receptiv ...	2016	26949132
assessment of injuries caused by anastrepha fraterculus (wied.) (diptera: tephritidae) on the incidence of bunch rot diseases in table grape.	anastrepha fraterculus (wied.) is the main insect pest of table grapes (vitis vinifera) in the southern region of brazil. in this study, we aimed to investigate the effect of fruit puncturing by adult females and larval infestation by a. fraterculus on the occurrence of bunch rot disease in the grape (cultivar "itália") by evaluating grapes (a) punctured for oviposition by females of a. fraterculus, sterilized in laboratory with novaluron (40 mg l(-1)) and further spray-inoculated separately wit ...	2016	26911161
cytogenetic analysis of the south american fruit fly anastrepha fraterculus (diptera:tephritidae) species complex: construction of detailed photographic polytene chromosome maps of the argentinian af. sp.1 member.	genetic and cytogenetic studies constitute a significant basis for understanding the biology of insect pests and the design and the construction of genetic tools for biological control strategies. anastrepha fraterculus is an important pest of the tephritidae family. it is distributed from southern texas through eastern mexico, central america and south america causing significant crop damage and economic losses. currently it is considered as a species complex; until now seven members have been ...	2016	27362546
biology of fopius arisanus (hymenoptera: braconidae) in two species of fruit flies.	fopius arisanus (sonan, 1932) (hymenoptera: braconidae) is an egg-larval parasitoid used in control programs of bactrocera dorsalis (hendel) and ceratitis capitata (wiedemann). in brazil, c. capitata and anastrepha fraterculus (wiedemann) are considered the main tephritid pests of exotic and indigenous fruits. the objective of this study was to study the biology of f. arisanus in c. capitata and a. fraterculus eggs of the two fruit fly species were used to determine the parasitism rate, number o ...	2016	27638954
irradiation of anastrepha fraterculus (diptera: tephritidae) eggs to inhibit fly emergence in the mass-rearing of diachasmimorpha longicaudata (hymenoptera: braconidae).	as the incidence of anastrepha fraterculus (wiedemann) has increased in southern brazil in the past 3 yr, an initiative to release sterile flies and parasitoids has started. in order to make feasible the mass-rearing of the parasitoid diachasmimorpha longicaudata (ashmed), this study investigated the suitability of a. fraterculus larvae derived from irradiated eggs as host for d. longicaudata two different ages of a. fraterculus eggs (24 and 48 h old) were analyzed for hatchability after the exp ...	2016	27638956
molecular evolution of odorant-binding proteins gene family in two closely related anastrepha fruit flies.	odorant-binding proteins (obps) are of great importance for survival and reproduction since they participate in initial steps of the olfactory signal transduction cascade, solubilizing and transporting chemical signals to the olfactory receptors. a comparative analysis of obps between closely related species may help explain how these genes evolve and are maintained under natural selection and how differences in these proteins can affect olfactory responses. we studied obp genes in the closely r ...	2016	27716035
temporal diversity and abundance patterns of parasitoids of fruit-infesting tephritidae (diptera) in the argentinean yungas: implications for biological control.	a 4-yr study was done to analyze seasonal patterns underlying host plant-fruit fly-parasitoid interactions in a secondary forest in the argentinean yunga and its importance for the implementation of conservation and augmentative biological control. larval-pupal hymenopteran parasitoids associated with all host plants and fruit fly species were identified and the seasonal occurrence of fruit, infestation levels, parasitism percentage, and relative parasitoid abundance were determined. three fruit ...	2016	27436842
resolution of inter and intra-species relationships of the west indian fruit fly anastrepha obliqua.	the west indian fruit fly, anastrepha obliqua (diptera: tephritidae), is an economically important pest that inhabits areas of south and central america, mexico and the caribbean with occasional infestations in the southern united states. we examine intra-specific relationships within a. obliqua as well as interspecific relationships to other anastrepha species using a multi-locus data set comprising nine loci (seven nuclear, two mitochondrial) with 105 operational taxonomic units. the results b ...	2016	27126185
tephritid integrative taxonomy: where we are now, with a focus on the resolution of three tropical fruit fly species complexes.	accurate species delimitation underpins good taxonomy. formalization of integrative taxonomy in the past decade has provided a framework for using multidisciplinary data to make species delimitation hypotheses more rigorous. we address the current state of integrative taxonomy by using as a case study an international project targeted at resolving three important tephritid species complexes: bactrocera dorsalis complex, anastrepha fraterculus complex, and ceratitis far (c. fasciventris, c. anona ...	2017	27813666
wolbachia strains in cryptic species of the anastrepha fraterculus complex (diptera, tephritidae) along the neotropical region.	infection by wolbachia was described previously in eleven species of anastrepha fruit flies some of which are important pests of fruticulture. one such species is the nominal anastrepha fraterculus, the south american fruit fly, which actually comprises a complex of cryptic species. the suggestions of using wolbachia for the control of these pest species, make imperative a more precise characterization of the existing strains of the bacteria. in this study, population samples of the a. fratercul ...	2017	27914738
expanding the view of clock and cycle gene evolution in diptera.	we expanded the view of clock (clk) and cycle (cyc) gene evolution in diptera by studying the fruit fly anastrepha fraterculus (afra), a brachycera. despite the high conservation of clock genes amongst insect groups, striking structural and functional differences of some clocks have appeared throughout evolution. clk and cyc nucleotide sequences and corresponding proteins were characterized, along with their mrna expression data, to provide an evolutionary overview in the two major groups of dip ...	2017	28234413
evaluation of food lures for capture and monitoring of anastrepha fraterculus (diptera: tephritidae) on temperate fruit trees.	the anastrepha fraterculus (wiedemann) (diptera: tephritidae) is the main pest of fruit trees grown in temperate climates in the southern region of brazil. the objective of this work was to evaluate the efficiency of the major commercial food lures used in brazil for trapping and monitoring of a. fraterculus in plum, pear, and feijoa orchards. the assessed lures were hydrolyzed proteins of animal origin (ceratrap) and plant origin (bioanastrepha), torula yeast + borax (torula), and grape juice. ...	2017	28334322
patterns of inner chorion structure in anastrepha (diptera: tephritidae) eggs.	the inner chorion structure of anastrepha eggs from 16 species of various infrageneric taxonomic groups is described by scanning and transmission electron microscopy. the layers of the chorion, the outer egg membrane, are structurally similar. furthermore, an additional trabecular layer (atl) that exists in some species, together with other characteristics, facilitates the recognition of four patterns of chorion structuring: pattern i, in which the atl layer is absent, is found in anastrepha ami ...	2017	27847335
isolation of entomopathogenic nematodes in an apple orchard in southern brazil and its virulence to anastrepha fraterculus (diptera: tephritidae) larvae, under laboratory conditions.	entomopathogenic nematodes (epns) are a promising alternative to integrated control in many fruit pests. few studies were made on the relationship of anastrepha fraterculus natural population with native epns population and other biotic and abiotic factors. the aim of this work was to verify the occurrence of endemic nematodes in an apple orchard, concerning environmental conditions and technical procedure, and access isolates virulence to a. fraterculus larvae. the experiment was conducted duri ...	2017	27383002
identifying anastrepha (diptera; tephritidae) species using dna barcodes.	molecular identification of fruit flies in the genus anastrepha (diptera; tephritidae) is important to support plant pest exclusion, suppression, and outbreak eradication. morphological methods of identification of this economically important genus are often not sufficient to identify species when detected as immature life stages. dna barcoding a segment of the mitochondrial cytochrome oxidase i gene has been proposed as a method to identify pests in the genus. the identification process for the ...	2017	29202187
evolutionary coincidence of adaptive changes in exuperantia and the emergence of bicoid in cyclorrhapha (diptera).	the great radiation in the infraorder cyclorrhapha involved several morphological and molecular changes, including important changes in anterior egg development. during drosophila oogenesis, exuperantia (exu) is critical for localizing bicoid (bcd) messenger rna (mrna) to the anterior region of the oocyte. because it is phylogenetically older than bcd, which is exclusive to cyclorrhapha, we hypothesize that exu has undergone adaptive changes to enable this new function. although exu has been wel ...	2017	28894941
male sexual enhancement after methoprene treatment in anastrepha fraterculus (diptera: tephritidae): a sustained response that does not fade away after sexual maturation.	the juvenile hormone (jh) of insects triggers physiological changes related to reproduction in adults of both sexes. methoprene is a sesquiterpene with some effects that are analogous to those of jh. treatments with methoprene accelerate sexual maturation in males of the south american fruit fly anastrepha fraterculus, giving young males a mating advantage over non-treated males of the same age. here, we evaluated the effects of methoprene treatment on a. fraterculus males after the sexual matur ...	2017	28623148
experimental hybridization and reproductive isolation between two sympatric species of tephritid fruit flies in the anastrepha fraterculus species group.	among tephritid fruit flies, hybridization has been found to produce local adaptation and speciation, and in the case of pest species, induce behavioral and ecological alterations that can adversely impact efficient pest management. the fraterculus species group within anastrepha (diptera: tephritidae), is a rapidly radiating aggregate, which includes cryptic species complexes, numerous sister species, and several pest species. molecular studies have highlighted the possibility of introgression ...	2017	28586142
odorant-binding proteins expression patterns in recently diverged species of anastrepha fruit flies.	we studied two species of closely related south american fruit flies, anastrepha fraterculus and anastrepha obliqua which, despite being able to interbreed, still show some ecological and reproductive differences. because part of these differences, such as host and mate preferences, may be related to olfactory perception, we focused our investigation on the differential expression of odorant-binding protein (obp) gene family, which participate in initial steps of the olfactory signal transductio ...	2017	28526847
oviposition of fruit flies (diptera: tephritidae) and its relation with the pericarp of citrus fruits.	fruit flies (diptera: tephritidae) represent a threat to fruit growing worldwide, mainly the citrus culture, however, biological studies show that fruit flies are not perfectly adapted to this host. this study investigated oviposition of anastrepha fraterculus (wiedemann, 1830) and ceratitis capitata (wiedemann, 1824) and its relation with the pericarp of citrus fruits. we evaluated the relationship between depth of oviposition of a. fraterculus and c. capitata and epicarp thickness of orange [c ...	2017	29091115
biological activity of bacillus thuringiensis (bacillales: bacillaceae) in anastrepha fraterculus (diptera: tephritidae).	anastrepha fraterculus (wiedemann) (diptera: tephritidae) is considered to be one of the major pest insects in fruit orchards worldwide. bacillus thuringiensis berliner (bacillales: bacillaceae) strains are widely used as biological control agents and show high biological activity against different insect species. the objective of this study was to evaluate the biological activity of different strains of b. thuringiensis against a. fraterculus larvae and adults. bioassays were performed using su ...	2018	29361000
evidence of adaptive evolution and relaxed constraints in sex-biased genes of south american and west indies fruit flies (diptera: tephritidae).	several studies have demonstrated that genes differentially expressed between sexes (sex-biased genes) tend to evolve faster than unbiased genes, particularly in males. the reason for this accelerated evolution is not clear, but several explanations have involved adaptive and nonadaptive mechanisms. furthermore, the differences of sex-biased expression patterns of closely related species are also little explored out of drosophila. to address the evolutionary processes involved with sex-biased ex ...	2018	29346618