Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| isolation and characterization of vibrio carchariae, a causative agent of gastroenteritis in the groupers, epinephelus coioides. | an outbreak of serious mortality among the cultured groupers epinephelus coioides, characterized by a swollen intestine containing yellow fluid, occurred in the summer of 1993 in taiwan. a motile strain emi82kl was isolated from the intestinal yellow fluid of the moribund groupers with tryptic soy agar supplemented with 2% nacl and/or thiosulfate citrate bile salt sucrose agar. this strain was characterized and identified as vibrio carchariae and was susceptible to chloramphenicol, doxycycline-h ... | 1997 | 9216885 |
| control of parasites in cultured marine finfishes in southeast asia--an overview. | mariculture in southeast asia began in the 1970s and expanded rapidly during the 1980s, with the commercial hatchery production of the seabass lates calcarifer. other important cultured species were epinephelus coioides, epinephelus malabaricus, lutjanus johni, and lutjanus argentimaculatus. intensification in the polyculture of these species and the large-scale international movement of fingerlings or juveniles, as well as the rapid expansion and concentration of fish farms, have caused severe ... | 1997 | 9394188 |
| synthesis of 17,20alpha/beta-dihydroxy-4-pregnen-3-one and 5beta-pregnanes in spermatozoa of primary and 17alpha-methyltestosterone-induced secondary male grouper (epinephelus coioides). | the in vitro metabolism of [3h]17-hydroxyprogesterone by spermatozoa of primary and 17alpha-methyltestosterone (mt)-induced secondary male grouper (epinephelus coioides) has been examined. with only the labeled precursor, the four predominant 5beta-pregnanes (17, 20alpha-dihydroxy-5beta-pregnan-3-one; 5beta-pregnan-3alpha,17, 20alpha-triol; 3alpha,17-dihydroxy-5beta-pregnan-20-one; and 17hydroxy-5beta-pregnan-3,20-dione) accounted for 75% of the total product yield, with the remainder comprising ... | 1998 | 9748397 |
| thyroid hormones promote early metamorphosis in grouper (epinephelus coioides) larvae. | the response of grouper larvae to the thyroid hormones, thyroxine (t4) and triiodothyronine (t3), was examined. two-, 3-, and 4-week-old grouper larvae were reared in seawater containing either t4 or t3 at 0.01, 0.1, and 1 ppm. t4 or t3 induced metamorphosis in all age groups in a dose-dependent manner. regardless of the size of the larvae, metamorphosis was completed in 2 days in larvae treated with 1 ppm of either t4 or t3; 3-4 days in larvae exposed to 0.1 ppm; and 5-6 days in larvae immersed ... | 1998 | 9748398 |
| protease and virulence of the extracellular products produced by vibrio carchariae after growth on various media. | protease and virulence of the extracellular products (ecp) of vibrio carchariae strain emi82kl, a causative agent of gastroenteritis in epinephelus coioides, cultured on different media were studied. the bacteria grown on peptone agar, nutrient agar or brain heart infusion agar produced higher protease activities than that grown on tryptic soy agar (tsa) in terms of protein content. the addition of ethylenediamine di(o-hydroxyphenylacetic acid) or horse serum in tsa enhanced the ecp protease pro ... | 1999 | 10431390 |
| temperature effect on nervous necrosis virus infection in grouper cell line and in grouper larvae. | this preliminary study elucidates the in vitro and in vivo effects of temperature on grouper nervous necrosis virus (gnnv) infection. a novel continuous cell line derived from the fin tissue of a grouper (epinephelus coioides, hamilton), named as gf-1 cell line, was used. cytopathic effect was observed in gnnv-infected gf-1 cells incubated at 24-32 degrees c after viral adsorption, but not at 20 degrees c or 37 degrees c even though the viral adsorption temperature was 28 degrees c. viral protei ... | 1999 | 10509721 |
| corticosteroid biosynthesis in vitro by testes of the grouper (epinephelus coioides) after 17alpha-methyltestosterone-induced sex inversion. | this study reports the unique compartmentalization of cortisol and 11-deoxycortisol biosynthesis in vitro from [(3)h]17alpha-hydroxyprogesterone (17p) in testicular tissues of groupers after sex inversion induced by 17alpha-methyltestosterone (mt). before mt implantation, the ovarian tissues produced only nonpolar metabolites. following sex inversion some 6 months later, synthesis of these nonpolar metabolites was not detectable. instead, cortisol and 11-deoxycortisol, with yields of about 3% an ... | 2000 | 11074458 |
| increased 21-hydroxylase and shutdown of c(17,20) lyase activities in testicular tissues of the grouper (epinephelus coioides) during 17alpha-methyltestosterone-induced sex inversion. | the metabolism in vitro of [(3)h]17-hydroxyprogesterone by gonadal tissues of the grouper (epinephelus coioides) during 17alpha-methyltestosterone (mt)-induced female-to-male sex inversion was examined. in the female phase, c(17,20) lyase, 5beta-reductase, 3alpha/beta-hsd, 20beta-hsd, and 17beta-hsd activities resulted in the biosynthesis of 5beta-pregnans and 5beta-androstanes (including 5beta-androstane-3alpha/beta, 17beta-diol, 3alpha/beta, 17alpha-dihydroxy-5beta-pregnen-20-one, and 5beta-an ... | 2002 | 12093117 |
| pathogenesis of gastroenteritis caused by vibrio carchariae in cultured marine fish. | serious mortality among the cultured grouper epinephelus coioides, characterized by a swollen intestine containing yellow fluid (gastroenteritis), occurred in 1993 in taiwan. a bacterium isolated from the intestinal fluid and head kidney of moribund groupers was identified as vibrio carchariae. since then, the same vibrio species has also been isolated from moribund black sea bream acanthopagrus schlegeli, yellowfin sea bream a. latus, japanese sea bass lateolabrax japonicus, and red drum sciaen ... | 2002 | 14961260 |
| molecular cloning, expression of orange-spotted grouper goose-type lysozyme cdna, and lytic activity of its recombinant protein. | lysozyme acts as a non-specific innate-immunity molecule against the invasion of bacteria pathogens. a leukocyte cdna library of orange-spotted grouper epinephelus coioides was constructed and the goose-type (g-type) lysozyme cdna was isolated. the complete cdna consists of an open reading frame of 585 bp encoding a protein of 194 amino acids. this protein shows a 72.2% amino acid sequence identity with the flounder g-type lysozyme. similar to most other species, the glu catalytic residue in g-t ... | 2003 | 12911059 |
| disposition of 3-(4-cyano-2-oxobutylidene amino)-2-oxazolidone, a cyano-metabolite of furazolidone, in furazolidone-treated grouper. | the cyano-metabolite of furazolidone (fz), 3-(4-cyano-2-oxobutylidene amino)-2-oxazolidone, was isolated from the mixture of fz incubated with the post-9000 g hepatic supernatant of grouper. its structure was confirmed by mass spectrometric and nuclear magnetic resonance spectroscopic studies. thereafter, the disposition of the cyano-metabolite in the orange-spotted grouper (epinephelus coioides) after oral and bath treatment with fz was investigated. qualitative and quantitative analyses of cya ... | 2003 | 12623646 |
| sequence and expression of a cdna encoding both pituitary adenylate cyclase activating polypeptide and growth hormone-releasing hormone in grouper (epinephelus coioides). | both pituitary adenylate cyclase activating polypeptide pacap and growth hormone-releasing hormone ghrh belong to the vasoactive intestinal polypeptide- glucagon-secretin family. they are encoded on the same gene in vertebrates (birds, amphibian, fish). although the gene encoding pacap and ghrh has been cloned in other fish species, characterization of this gene in the commercially important grouper (epinephelus coioides) has not been previously reported. in this study, the ghrh/pacap cdna was c ... | 2003 | 12958662 |
| androgens stimulate sex change in protogynous grouper, epinephelus coioides: spawning performance in sex-changed males. | we examined the efficacy of androgens (1.0 mg/kg body mass), testosterone (t), 11-ketotestosterone (11-kt), 17alpha-methyltestosterone (mt), testosterone propionate (tp) or androgen mixture (t, mt and tp in an equal ratio), for induction of sex change in protogynous orange-spotted grouper, epinephelus coioides. the spawning performance in sex-changed males was also investigated. mt and androgen mixture at a dose of 1.0 mg/kg bw induced a sex transition and completion of spermatogenesis up to the ... | 2003 | 12927912 |
| two distinct cytochrome p450 aromatases in the orange-spotted grouper (epinephelus coioides): cdna cloning and differential mrna expression. | the cdna sequences encoding two distinct cytochrome p450 aromatases, namely p450aromb and p450aroma, were isolated from brain and ovary cdna libraries of the orange-spotted grouper, respectively. the p450aromb cdna consists of 1892 bp, and the open reading frame (orf) encodes a putative protein of 506 amino acids. the p450aroma cdna consists of 1836 bp, and the orf encodes a putative protein of 518 amino acids. northern blot analysis revealed a transcript of about 1.9 kb for p450aromb in the bra ... | 2004 | 15544929 |
| stimulatory effects of gonadotropin-releasing hormone and dopamine on growth hormone release and growth hormone mrna expression in epinephelus coioides. | gonadotropin-releasing hormone (gnrh) and dopamine (da) can stimulate growth hormone (gh) release, but their effects on gh mrna synthesis are controversial and deficient in fish. orange-spotted grouper (epinephelus coioides) is a hermaphroditic marine fish with sex reversal. few data are available concerning the regulation of gh in grouper. in the present study, the effects of gnrh and da on gh release and gh mrna expression were determined using pituitary fragments of orange-spotted grouper und ... | 2004 | 15497048 |
| cdna cloning and mrna expression of a ftz-f1 homologue from the pituitary of the orange-spotted grouper, epinephelus coioides. | a ftz-f1 homologue was cloned from the pituitary cdna library of the orange-spotted grouper. the full-length cdna of the orange-spotted grouper ftz-f1 spanned 1735 bp including a poly (a) tail. the open reading frame encodes a protein of 468 amino acids. sequence analysis indicated that it had a structure typical of the orphan nuclear receptor superfamily, and the ftz-f1 box, a characteristic of the ftz-f1 family. phylogenetic analysis indicated that the orange-spotted grouper ftz-f1 was closely ... | 2004 | 15286949 |
| cdna sequence and spatio-temporal expression of prolactin in the orange-spotted grouper, epinephelus coioides. | the cdna sequence encoding the prolactin (prl) gene of the orange-spotted grouper was obtained by randomly sequencing its pituitary cdna library. the full-length cdna of orange-spotted grouper prl consists of 1342 bp. the open reading frame encodes a protein of 212 amino acids, which consists of a putative signal peptide of 24 residues and a mature protein of 188 amino acids. northern blot analysis identified a single transcript of 1.4 kb in the pituitary gland, indicating perhaps the presence o ... | 2004 | 14980804 |
| two new blood flukes (digenea: sanguinicolidae) from epinephelinae (perciformes: serranidae) of the pacific ocean. | pearsonellum pygmaeus n. sp. is described from cromileptes altivelis (serranidae), the barramundi cod, from heron island (southern great barrier reef) and lizard island (northern great barrier reef). this new species differs from pearsonellum corventum (type and only species) in the combination of smaller overall body size, the relative distance of the brain from the anterior end, the relative lengths of both the oesophagus and the testis, the degree to which the testis extends outside the inter ... | 2004 | 15464442 |
| differential expression of thyroid-stimulating hormone beta subunit in gonads during sex reversal of orange-spotted and red-spotted groupers. | we have cloned and characterized the full-length cdna encoding thyroid-stimulating hormone beta-subunit (tshbeta) from orange-spotted grouper epinephelus coioides. it contains 913 nucleotides with an open reading frame encoding 146 amino acids with a 20 amino acid signal peptide. the grouper mature tshbeta has 75, 70, 61, 59, 41, 42 and 40% identities to that of rainbow trout, atlantic salmon, zebrafish, european eel, chicken, mouse and human, respectively. rt-pcr analysis indicated that the tsh ... | 2004 | 15196702 |
| susceptibility of juvenile humpback grouper cromileptes altivelis to grouper sleepy disease iridovirus (gsdiv). | susceptibility of juvenile humpback grouper cromileptes altivelis to the grouper sleepy disease iridovirus (gsdiv) was examined. gsdiv-containing inocula for challenge were obtained using a filtrate of spleen tissues from donor fish (orange-spotted grouper epinephelus coioides) infected with gsdiv. groups injected with the primary filtrate showed lower mortalities (30 to 60%) than groups receiving the 10(-4) diluted inoculum (90 to 100% mortality). this result was contrary to the expectation tha ... | 2004 | 15212286 |
| molecular cloning and mrna profile of insulin-like growth factor type 1 receptor in orange-spotted grouper, epinephelus coioides. | the insulin-like growth factor type 1 receptor (igf-ir) belongs to the tyrosine kinase (tk) receptor family. besides being mitogenic, igf-ir plays a crucial role in cell survival, transformation and maintenance of the malignant phenotype. in this study, we cloned the cdna from the hypothalamus of the orange-spotted grouper (epinephelus coioides) using reverse transcription pcr (rt-pcr) and the rapid amplification of cdna ends (race) method. the deduced amino acid sequence showed that the recepto ... | 2005 | 15880261 |
| chlorinated hydrocarbons in marine biota and coastal sediments from the gulf and gulf of oman. | the spatial distribution of various organochlorinated compounds was investigated in the gulf and gulf of oman based on marine biota (fish and various bivalves) and coastal sediment collected in bahrain, oman, qatar and the united arab emirates (uae) during 2000-2001. several potential organic contaminants from agricultural (e.g., ddt and its breakdown products, lindane, endrin, dieldrin, endosulfan) and industrial (pcbs) sources were measured. sediment burdens for all compounds, even for an appa ... | 2005 | 16115501 |
| study of an outbreak of ciguatera fish poisoning in hong kong. | ciguatera fish poisoning (cfp) has been a significant and increasing public health problem in hong kong since 1980s. with growing demand for imported live coral fishes, the number of people who suffered from this disease has also been increasing. an outbreak of cfp in 2004 was the second most prominent in record as compared with the most significant one that occurred in 1998. in 2004, out of a total of 823 reported food poisoning outbreaks involving 3159 persons, 65 incidents (7.9%) affecting 24 ... | 2005 | 16085209 |
| polygenic expression of somatostatin in orange-spotted grouper (epinephelus coioides): molecular cloning and distribution of the mrnas encoding three somatostatin precursors. | in the present study, three preprosomatostatin (pss) cdnas were characterized from hypothalamus of orange-spotted grouper epinephelus coioides. the first cdna encodes a 123-amino acid protein (pssi) that contains the ss14 sequence at its c-terminal extremity and that is identical to that of pssi of human and other vertebrates. the second cdna encodes a 127-amino acid protein (pssii) that contains the ss28 sequence with [tyr7, gly10]-ss14 at its c-terminus. the third cdna encodes a 110-amino acid ... | 2005 | 16054749 |
| complete genome sequence analysis of an iridovirus isolated from the orange-spotted grouper, epinephelus coioides. | orange-spotted grouper iridovirus (osgiv) was the causative agent of serious systemic diseases with high mortality in the cultured orange-spotted grouper, epinephelus coioides. here we report the complete genome sequence of osgiv. the osgiv genome consists of 112,636 bp with a g+c content of 54%. 121 putative open reading frames (orf) were identified with coding capacities for polypeptides varying from 40 to 1168 amino acids. the majority of osgiv shared homologies to other iridovirus genes. phy ... | 2005 | 15964605 |
| pseudorhabdosynochus summanoides n. sp. (monogenea: diplectanidae) from epinephelus coioides in dapeng bay, south china sea, with observations on several similar species of pseudorhabdosynochus yamaguti, 1958. | one new species of diplectanid monogenean, pseudorhabdosynochus summanoides n. sp., is reported and described from the marine fish epinephelus coioides off nan'ao shenzhen, china. p. summanoides differs from its closest relative, p. summanae (young, 1968), by the shape of its vaginal hard-parts, which have a tightly twisted distal region and an accessory patch on the proximal curve. during the course of this work, type-material of several species of pseudorhabdosynochus was examined and aspects ... | 2005 | 16315082 |
| description of pseudorhabdosynochus seabassi sp. n. (monogenea: diplectanidae) from lates calcarifer and revision of the phylogenetic position of diplectanum grouperi (monogenea: diplectanidae) based on rdna sequence data. | pseudorhabdosynochus seabassi sp. n. (monogenea: diplectanidae) from the gill filaments of lates calcarifer bloch, a marine teleost fish held in floating sea cages in guangdong province, china, is described based on morphological observations and molecular data. the shapes of the male copulatory organs (mco) of pseudorhabdosynochus spp. were the focus of this study. the typical proximal part of the mco in most species of pseudorhabdosynochus is reniform, heavily sclerotized, and divided into fou ... | 2005 | 16270804 |
| trace element concentrations in fish, surficial sediments and water from northern part of the persian gulf. | concentrations of cadmium, lead, nickel and vanadium were determined in water, surficial sediments and the muscle of three demersal fish species (epinephelus coioides, psettodes erumei and solea elongate) from 15 sampling sites in the northern part of persian gulf. concentrations of the elements were determined using inductively coupled plasma mass spectrometer. there were no significant differences among the sampling sites in cd and pb levels in the sediments. the highest concentrations of ni a ... | 2005 | 16240204 |
| molecular cloning, tissue distribution, and ontogeny of mrna expression of growth hormone in orange-spotted grouper (epinephelus coioides). | a full-length cdna encoding for growth hormone (gh) was cloned from orange-spotted grouper (epinephelus coioides) pituitary using reverse transcription and rapid amplification of cdna ends (race). the gh precursor cdna consists of 956 bp in size with a 85 bp 5'-untranslated region and 259 bp 3'-untranslated region. the 612 bp open reading frame encodes a 204 amino acid (aa) protein, which represents the precursor of grouper gh composed of a 17 aa signal peptide followed by a 187 aa mature gh pol ... | 2005 | 16055124 |
| description of pseudorhabdosynochus shenzhenensis n. sp. (monogenea: diplectanidae) and redescription of p. serrani yamaguti, 1953 from epinephelus coioides off dapeng bay, shenzhen, china. | one new species of diplectanid, pseudorhabdosynochus shenzhenensis n. sp., is reported and described from the marine fish epinephelus coioides off nan'ao, shenzhen, china. it can be differentiated from previously described species of the same genus by features of the haptoral and vaginal hard-parts. a second species, p. serrani yamaguti, 1953, originally described from serranus sp. from the western pacific ocean off the celebes (now called sulawesi), is redescribed based on new material from e. ... | 2005 | 17089747 |
| molecular cloning and sequence analysis of full-length growth hormone cdnas from six important economic fishes. | in this study,the full-length cdnas of gh (growth hormone) gene was isolated from six important economic fishes, siniperca kneri, epinephelus coioides, monopterus albus, silurus asotus, misgurnus anguillicaudatus and carassius auratus gibelio bloch. it is the first time to clone these gh sequences except e. coioides gh. the lengths of the above cdnas are as follows: 953 bp, 1 023 bp, 825 bp, 1 082 bp, 1 154 bp and 1 180 bp. each sequence includes an orf of about 600 bp which encodes a protein of ... | 2005 | 15715434 |
| molecular cloning and expression pattern of 14 kda apolipoprotein in orange-spotted grouper, epinephelus coioides. | a novel fish-specific apolipoprotein (apo-14 kda) has been recently cloned from eel and pufferfish. however, its expression pattern has not been elucidated. in this study, ecapo-14 has been screened from hypothalamic cdna library of male orange-spotted grouper, which shows 62.9%, 51%, 46.9%, 43.2%, and 31.9% identities to apo-14 of european flounder, pufferfish, japanese eel, gibel carp, and grass carp, respectively. rt-pcr analysis reveals that this gene is first transcribed in neurula embryos ... | 2005 | 16246612 |
| cdna cloning and mrna expression of neuropeptide y in orange spotted grouper, epinephelus coioides. | a full-length cdna encoding the neuropeptide y (npy) was cloned from the hypothalamus of orange spotted grouper (epinephelus coioides) by rapid amplification of cdna ends approaches. the npy cdna sequence is 688 bp long and has an open reading frame of 300 bp encoding prepro-npy with 99 amino acids. the deduced amino acid sequences contain a 28-amino-acids signal peptide followed by a 36-amino-acids mature npy peptide. mrna expression of npy was determined using semi-quantitative rt-pcr followed ... | 2005 | 16005251 |
| molecular and morphological evidence indicates that pseudorhabdosynochus lantauensis (monogenea: diplectanidae) represents two species. | sequences of the first internal transcribed spacer (its-1) and the d1-d3 domains of the large subunit (lsu) of the ribosomal dna (rdna) were determined for multiple specimens of 4 operational taxonomic units (otus) of the monogenean, pseudorhabdosynochus lantauensis. otus were defined based on their collecting localities, host and/or morphological characteristics. all p. lantauensis specimens of one group (otus 1 and 3) differed in their sequences of the its-1 and partial lsu rdna when compared ... | 2005 | 15977904 |
| molecular characterization of rockfish (sebastes schlegeli) gonadotropin subunits and their mrna expression profiles during oogenesis. | in an attempt to understand the reproductive regulation in viviparous teleosts, gonadotropin (gth) subunit cdnas were characterized and the expression levels of gth subunit mrnas in the pituitary glands of the rockfish, sebastes schlegeli (scorpaeniformes, scorpaenidae), were examined by northern blot analysis. the complete sequences of rockfish gth subunits (gthalpha, fshbeta, and lhbeta) were determined by rapid amplification of cdna ends (race) and nucleotide sequencing. based on the race ana ... | 2005 | 15804515 |
| molecular and expression characterization of three gonadotropin subunits common alpha, fshbeta and lhbeta in groupers. | a smart cdna plasmid library was constructed from protogyous greasy grouper (epinephelus coioides) pituitary, and the full-length cdnas of three gonadotropin (gth) subunits common alpha, fshbeta and lhbeta were cloned and sequenced from the library. the nucleotide sequences of common alpha, fshbeta and lhbeta subunit cdnas are 647, 594 and 574 bp in length, and encode for mature peptides of 94, 99 and 115 aa, respectively. high homology was observed by amino acid sequence alignment and identity ... | 2005 | 15767044 |
| cloning of an orange-spotted grouper (epinephelus coioides) mx cdna and characterisation of its expression in response to nodavirus. | molecular cloning and nucleotide sequencing of cdna encoding an orange-spotted grouper (epinephelus coioides) homolog of mx ("osgmx") was conducted and its possible role in fish immunity was analysed. similar to mammalian mx, the osgmx are members of a family of interferon-inducible genes that are expressed by cells in response to nodavirus and iridovirus naturally-infected. expression of osgmx mrna was noticeably upregulated in all tissues by nodavirus naturally-infected grouper. the transcript ... | 2006 | 15967681 |
| temporal changes of ethoxyresorufin-o-deethylase (erod) activities and lysosome accumulation in intestine of fish on chronic exposure to dietary benzo[a]pyrene: linking erod induction to cytological effects. | temporal changes of intestinal and hepatic ethoxyresorufin-o-deethylase (erod) activities and quantitative changes of secondary and tertiary (e.g., 2 degrees/3 degrees) lysosomes in enterocytes were compared for the juvenile grouper (epinephelus coioides) on chronic exposure to foodborne benzo[a]pyrene (bap) at two environmentally realistic levels (0.25 and 12.5 microg/g fish/d) over a four-week exposure and four-week depuration period. intestinal erod induction was rapid (within 3 d) and sustai ... | 2006 | 17022398 |
| identification of a novel c2 domain factor in ovaries of orange-spotted grouper (epinephelus coioides). | follicle consists of an oocyte and a lot of surrounding follicular cells, and significant interactions exist between the oocyte and the somatic cells. in this study, a novel cdna has been screened from a subtractive cdna library between tail bud embryos and blastula embryos in the protogynous hermaphrodite orange-spotted grouper (epinephelus coioides). its full-length cdna is 821 bp, and has an orf of 414 bp for encoding a peptide of 137 aa, which shows 38%, 37%, 33%, and 33% homology with 4 put ... | 2006 | 16459120 |
| characterization of serum immunoglobulin m of grouper and cdna cloning of its heavy chain. | immunoglobulin m (igm) from the whole serum of grouper fish, epinephelus coioides was purified by affinity chromatography using protein a-sepharose column. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) under reducing conditions revealed that the relative molecular masses (mr) of the equimolar heavy and light chains of igm were 78,000 and 27,000, respectively. the cdnas encoding igm heavy chain comprising its variable (vh) and constant (ch) regions have been cloned and sequ ... | 2006 | 16199094 |
| changes in mrna expression of grouper (epinephelus coioides) growth hormone and insulin-like growth factor i in response to nutritional status. | growth hormone (gh) and insulin-like growth factor-i (igf-i) are key links to nutritional condition and growth regulation in teleost. to understand the endocrine mechanism of growth regulation in grouper, we cloned the cdnas for grouper gh and igf-i and examined their mrna expression during different nutritional status. grouper gh cdna is 936 base pairs (bp) long excluding the poly-a tail. it contained untranslated regions of 85 and 231bp in the 5'- and 3'-ends, respectively. it has an open read ... | 2006 | 16243324 |
| inhibition of nervous necrosis virus propagation by fish mx proteins. | mx proteins are interferon induced, antiviral proteins, expressed in response to treatment with double stranded rna or virus infection. here we report the cloning, sequencing, and antiviral property of three forms of mx genes, mxi, mxii, and mxiii from grouper (epinephelus coioides). multiple comparison of grouper mx amino acid sequences revealed the conservation of mx putative gtp-binding domain, dynamin family signature, and leucine zipper motif. we have established a new cell line, grouper br ... | 2006 | 17069756 |
| development and characterization of a new tropical marine fish cell line from grouper, epinephelus coioides susceptible to iridovirus and nodavirus. | the development and characterization of a new tropical marine fish cell line (gs), derived from the spleen of orange spotted grouper, epinephelus coioides is described. the gs cells grow well in leibovitz's l-15 medium supplemented with 10% foetal bovine serum, and have been subcultured more than 200 times. the optimal growth temperature was 27 degrees c. the gs cell culture consisted of mostly fibroblastic cells. the modal diploid chromosome number was 48. gs cell cultures showed advanced cytop ... | 2006 | 16137774 |
| comparative genomic analysis of the family iridoviridae: re-annotating and defining the core set of iridovirus genes. | members of the family iridoviridae can cause severe diseases resulting in significant economic and environmental losses. very little is known about how iridoviruses cause disease in their host. in the present study, we describe the re-analysis of the iridoviridae family of complex dna viruses using a variety of comparative genomic tools to yield a greater consensus among the annotated sequences of its members. | 2007 | 17239238 |
| crustacean fish parasites from segara anakan lagoon, java, indonesia. | the present study is the first investigation on ectoparasites of commercial important fish from segara anakan, a brackish water lagoon located at the southern coast of java, indonesia. eight economically important marine fish species (mugil cephalus, siganus javus, scatophagus argus, caranx sexfasciatus, lutjanus johnii, eleutheronema tetradactylum, johnius coitor, and epinephelus coioides) were examined for crustacean parasites. prevalence and intensity data for each parasite species are given, ... | 2007 | 17219222 |
| protective immunity in grouper (epinephelus coioides) following exposure to or injection with cryptocaryon irritans. | the protective immunity of grouper (epinephelus coioides) against cryptocaryon irritans was determined after immunisation by surface exposure or intraperitoneal injection. specific antibody titres of immunised fish serum and skin culture supernatant were determined by enzyme-linked immunosorbent assay and immobilisation assays. specific antibody can be detected in some immunised fish at week 1 and in all immunised fish at week 2, and the peaks were between weeks 4-6. specific antibody was still ... | 2007 | 17123830 |
| production of recombinant orange-spotted grouper (epinephelus coioides) luteinizing hormone in insect cells by the baculovirus expression system and its biological effect. | the cdna sequence encoding orange-spotted grouper lhb (lhbeta) and cga (gthalpha) subunits were cocloned into baculovirus transfer vectors and expressed in insect sf9 cells. the results showed that two bands of 15.6 kda and 11.4 kda could be detected by sds-page and a band of 30 kda could be detected by native page. the recombinant grouper lh (rglh) could stimulate the secretion of testosterone (t) and estradiol-17beta (e2) from the gonad in a static incubation system in a time-dependent, but no ... | 2007 | 17021348 |
| the immunostimulatory effects of sodium alginate and iota-carrageenan on orange-spotted grouper epinephelus coicoides and its resistance against vibrio alginolyticus. | the lysozyme activity, alternative complement activity (ach50), respiratory burst, sod (superoxide dismutase) activity and phagocytic activity of orange-spotted grouper epinephelus coicoides were examined when the fish were injected intraperitoneally with sodium alginate at 10, 20, 30mgkg-1 and iota-carrageenan at 10, 20, 30mgkg-1, respectively after 24, 72 and 120 h. serum ach50 increased directly with dose after 24 and 72 h for both sodium alginate and iota-carrageenan treatments. the fish tha ... | 2007 | 16784873 |
| gene expression and localization of the epinecidin-1 antimicrobial peptide in the grouper (epinephelus coioides), and its role in protecting fish against pathogenic infection. | epinecidin-1 is an antimicrobial peptide and plays a vital role in protecting fish against pathogenic infection. as a mimic of a grouper epinecidin-1 peptide, it has tertiary structures that closely resemble those of pleurocidin found in the winter flounder (pleuronectes americanus). the tissue-specific, lipopolysaccharide (lps)-stimulation-specific, and poly(i):poly(c)-stimulation-specific expressions of the grouper (epinephelus coioides) epinecidin-1 antimicrobial peptide were determined using ... | 2007 | 17570764 |
| molecular cloning and gene expression of the gonadotropin-releasing hormone receptor in the orange-spotted grouper, epinephelus coioides. | the objective of this study was to investigate the molecular mechanisms of gonadotropin-releasing hormone receptor (gnrh-r) involved in the endocrine regulation of reproduction in the orange-spotted grouper, epinephelus coioides. the full-length cdna encoding gnrh-r type i was successfully cloned from the pituitary by reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end (race) methods in the grouper. the complete gnrh-r type i cdna is 1607 bp, which includ ... | 2007 | 17329139 |
| molecular epidemiology and phylogenetic analysis of a marine fish infectious spleen and kidney necrosis virus-like (isknv-like) virus. | infectious spleen and kidney necrosis virus-like (isknv-like) virus causes a serious systemic disease with high morbidity and mortality of freshwater and marine fishes. based on the isknv putative major capsid protein (mcp), the vascular endothelial growth factor (vegf), the mrna capping enzyme (capping), and the tumor necrosis factor receptor-associated protein (tnfr) genes, primers were designed and used in pcr to determine the host range of isknv-like viruses. from the sampling of >1600 marin ... | 2007 | 17131065 |
| development and characterization of two new cell lines from milkfish (chanos chanos) and grouper (epinephelus coioides) for virus isolation. | two new cell lines, simh and sige, were derived from the heart of milkfish (chanos chanos), a euryhaline teleost, and from the eye of grouper (epinephelus coioides), respectively. these cell lines were maintained in leibovitz's l-15 supplemented with 20% fetal bovine serum (fbs). the simh cell line was subcultured more than 50 times over a period of 210 days and sige cell line has been subcultured 100 times over a period of 1 1/2 years. the simh cell line consists predominantly of fibroblastic-l ... | 2007 | 17216384 |
| myostatin gene organization and nodavirus-influenced expression in orange-spotted grouper (epinephelus coioides). | the relationship(s) between nodavirus infection and myostatin expression in the skeletal muscle tissue of grouper is unclear. to investigate, the grouper (epinephelus coioides) myostatin gene was cloned and cdna was utilized to examine the expression of the gene in skeletal muscle and serum of healthy (uninfected) grouper and fish naturally infected with nodavirus. the myostatin gene comprises three exons and two introns and is transcribed as a 2778-bp mrna length that encodes a 376-aa precursor ... | 2007 | 20483295 |
| identification and characterization of a mbp isoform specific to hypothalamus in orange-spotted grouper (epinephelus coioides). | myelin basic protein (mbp), as a major component of the myelin sheath, has been revealed to play an important role in forming and maintaining myelin structure in vertebrate nervous system. in teleost, hypothalamus is an instinctive brain center and plays significant roles in many physiological functions, such as energy metabolism, growth, reproduction, and stress response. in comparison with other mbp identified in vertebrates, a smallest mbp is cloned and identified from the orange-spotted grou ... | 2007 | 17513086 |
| differential expression and dynamic changes of sox3 during gametogenesis and sex reversal in protogynous hermaphroditic fish. | sox3 has been suggested to play significant roles in gametogenesis and gonad differentiation of vertebrates, but the exact cellular localization evidence is insufficient and controversial. in this study, a protogynous hermaphrodite fish epinephelus coioides is selected to analyze ecsox3 differential expression and the expression pattern in both processes of oogenesis and spermatogenesis by utilizing the advantages that gonad development undergoes transition from ovary to intersexual gonad and th ... | 2007 | 17436330 |
| induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (epinephelus coioides) upon exposure to foodborne benzo[a]pyrene. | the sublethal toxicity of dietary benzo[a]pyrene, b[a]p, on fish growth and intestinal morphofunctional changes [as measured by epithelial turnover, cell proliferation, hyperplasia, de novo crypt formation and protein absorption efficiency (i.e. expression of proton/peptide co-transporter, pept-1, on the mucosal brush border)] were studied for the carnivorous orange-spotted grouper (epinephelus coioides). juvenile fish were force-fed daily with pellets containing environmentally realistic concen ... | 2007 | 17383024 |
| molecular cloning, characterization and distribution of two types of growth hormone receptor in orange-spotted grouper (epinephelus coioides). | the cdnas encoding two distinct growth hormone receptors (ghrs) were firstly cloned and sequenced from the liver of orange-spotted grouper (epinephelus coioides). the cdna of grouper ghr1 consisted of 2673-bp and encoded 658 amino acids, while the cdna of grouper ghr2 consisted of 2989-bp and encoded 577 amino acids. the two cdnas shared 78.6% identity in nucleotide sequence and 37.8% identity in deduced amino acid sequence. northern blot analysis demonstrated a single ghr1 transcript of approxi ... | 2007 | 17382945 |
| differential and spermatogenic cell-specific expression of dmrt1 during sex reversal in protogynous hermaphroditic groupers. | dmrt1 has been suggested to play different roles in sex determination and gonad differentiation, because different expression patterns have been reported among different vertebrates. the groupers, since their gonads first develop as ovary and then reverse into testis, have been thought as good models to study sex differentiation and determination. in this study, we cloned the full-length cdnas of dmrt1 gene from orange-spotted grouper (epinephelus coioides), and prepared corresponding anti-ecdmr ... | 2007 | 17092636 |
| the mrna expression of p450 aromatase, gonadotropin beta-subunits and ftz-f1 in the orange-spotted grouper (epinephelus coioides) during 17alpha-methyltestosterone-induced precocious sex change. | the orange-spotted grouper epinephelus coioides is a protogynous hermaphroditic fish, but the physiological basis of its sex change remains largely unknown. in the present study, the 2-year-old orange-spotted grouper was induced to change sex precociously by oral administration of 17alpha-methyltestosterone (mt, 50 mg/kg diet, twice a day at daily ration of 5% bodyweight) for 60 days. the serum testosterone levels were significantly elevated after mt treatment for 20 and 40 days as compared to c ... | 2007 | 17075797 |
| the food habit of epinephelus coioides (hamilton, 1822) in khuzestan coastal waters (persian gulf). | during january 2000 to march 2001, a large number of epinephelus coioides species was captured in main fishing areas of khuzestan province namely, busafe-liphe and bahrakan, north of persian gulf. the stomach contents of 394 individuals were analysed, in which 226 individuals had contained (trace-full) stomachs while 168 individuals had empty stomachs. the percentages of cv in males were more than in females. seventy three percent of stomachs contained fish 11% crab; 8.8% shrimp; 3.9% squid; 1.7 ... | 2007 | 19090275 |
| molecular cloning and expression analysis of interferon-gamma-inducible-lysosomal thiol reductase gene in orange-spotted grouper, epinephelus coioides. | in mammals, interferon-gamma-inducible-lysosomal thiol reductase (gilt) has been demonstrated to play a key role in the processing and presentation of mhc class ii-restricted antigen (ag) by catalyzing disulfide bond reduction, thus unfolding native protein ag and facilitating subsequent cleavage by proteases. in this study a cdna containing the orange-spotted grouper gilt (osggilt) coding sequence has been cloned and its complete sequence determined. the full-length cdna of osggilt gene is 1066 ... | 2007 | 17981053 |
| enhancement of hypoxia-induced gene expression in fish liver by the aryl hydrocarbon receptor (ahr) ligand, benzo[a]pyrene (bap). | fish in polluted coastal habitats commonly suffer simultaneous exposure to both hypoxia and xenobiotics. although the adaptive molecular responses to each stress have been described, little is known about the interaction between the signaling pathways mediating these responses. previous studies in mammalian hepatoma cell lines have shown that hypoxia-inducible factor (hif)- and/or aryl hydrocarbon receptor (ahr)-activated gene expression is suppressed following co-exposure to hypoxia and the hal ... | 2008 | 18945501 |
| molecular cloning, tissue distribution and expression profiles of thyroid hormone receptors during embryogenesis in orange-spotted grouper (epinephelus coioides). | three distinct thyroid hormone receptor (tr) cdnas (gtralphaa, gtralphab and gtrbeta) were cloned and sequenced in orange-spotted grouper (epinephelus coioides). the cdna of gtralphaa consisted of 1555-bp and encoded a putative protein of 416 amino acids, while the cdna of gtralphab consisted of 1459-bp and encoded 398 amino acids. the cdna of gtrbeta is 1470-bp in length and encoded 395 amino acids. the cdnas of gtralphaa and gtralphab had 68.7% identity in nucleotide sequence and 83.9% identit ... | 2008 | 18805420 |
| genetic population structure of red snapper (lutjanus malabaricus) and orange-spotted grouper (epinephelus coiodes) in brunei and sabah. | the genetic population structure of red snapper lutjanus malabaricus and orange-spotted grouper epinephelus coiodes in brunei and sabah was investigated using allozyme electrophoresis. samples were collected from three sites in brunei for e. coiodes and from three sites in brunei and sabah for l. malabaricus. a total of 22 loci and 16 loci were scored, respectively. the index of fixation (f(st) ) for the e. coiodes population was 0.176 but, in general, it lacked within-population structuring. th ... | 2008 | 21396070 |
| molecular cloning and mrna expression analysis of two gh secretagogue receptor transcripts in orange-spotted grouper (epinephelus coioides). | gh secretagogue receptor (ghsr) is the receptor of ghrelin, a circulating gh-releasing and appetite-inducing hormone. in this paper, two ghsr cdnas, gpghsr1a and gpghsr1b, were identified and characterized in a teleost, the orange-spotted grouper (epinephelus coioides). the gpghsr1a is 1512 bp in length with an open reading frame (orf) that encodes a protein of 383 amino acids with seven transmembrane (tm) domains, while the 1703 bp gpghsr1b contains an orf encoding for 303 amino acids with five ... | 2008 | 18753333 |
| whole-genome transcriptional profiles of a novel marine fish iridovirus, singapore grouper iridovirus (sgiv) in virus-infected grouper spleen cell cultures and in orange-spotted grouper, epinephulus coioides. | a dna microarray containing all singapore grouper iridovirus (sgiv) open reading frames (orfs) was constructed to map the viral gene transcriptional profiles in virus-infected grouper spleen (gs) cells and in spleen tissues of virus-infected grouper. the results showed that viral genes started to be transcribed as early as 1 h postinfection (p.i.), and followed by a rapid increasing gene expression along with virus infection in cell cultures. the three temporal kinetic classes (15 immediate-earl ... | 2008 | 18555886 |
| a homologue of sox11 predominantly expressed in the ovary of the orange-spotted grouper epinephelus coioides. | sox, a family of genes related to the mammalian sex-determining region y (sry) gene, are found throughout the animal kingdom and regulate diverse developmental processes including sex determination. the full-length sox11b cdna was cloned from the ovary of the orange-spotted grouper epinephelus coioides. this sequence is highly homologous to sox11 of other species and contained the signature features of mammalian sox11 homologues, except for the absence of pro-glu rich region and presence of two ... | 2008 | 18032080 |
| molecular cloning of myostatin gene and characterization of tissue-specific and developmental stage-specific expression of the gene in orange spotted grouper, epinephelus coioides. | in this article we report the molecular cloning and characterization of a nonmammalian myostatin (growth and differentiation factor-8, mstn) homolog from the orange spotted grouper (epinephelus coioides) by polymerase chain reaction (pcr) cloning. the grouper mstn gene consists of two introns [intron i (363 bp) and intron ii (811 bp)] flanked by three exons [exon i (379 bp), exon ii (371 bp) and exon iii (381 bp)]. a full-length cdna clone (2608 bp) of the mstn gene (genbank dq493889, nucleotide ... | 2008 | 17048071 |
| study of genetic diversity of orange-spotted grouper, epinephelus coioides, from thailand and indonesia using microsatellite markers. | genetic diversity of epinephelus coioides (hamilton, 1822), which inhabits coastal reefs from the western indian ocean to the western pacific ocean, was studied based on four polymorphic microsatellite loci. two hundred and fifty individuals were collected from two locations in thailand (nakornsrithammarat-n and trang-t) and four in indonesia (sibolga-s, lampung-l, jepara-j, and flores-f). the genetic variation of e. coioides was relatively low; the observed heterozygosities (ho) ranged between ... | 2008 | 16331343 |
| characterization and expression of the pepsinogen c gene and determination of pepsin-like enzyme activity from orange-spotted grouper (epinephelus coioides). | one pepsinogen c gene was cloned from the gastric mucosa of orange-spotted grouper (epinephelus coioides) for the first time. this gene consisted of nine exons interrupted by eight introns. the nucleotide sequence with the coding region and 3'-flanking region was also determined. the deduced polypeptide sequence was composed of a prosegment of 55 residues and a pepsin moiety of 332 residues. the putative substrate-binding subsites were well conserved among fish with the exception of the residue ... | 2008 | 17977766 |
| some characteristics of host-parasite relationship for cryptocaryon irritans isolated from south china. | a survey on the host range for the parasitic ciliate cryptocaryon irritans was carried out among the major maricultured fish species in the huizhou region of guangdong province in south china, and some characteristics of its host-parasite relationship were described. the survey showed that all ten investigated species of fish (representing six different families) were infected with c. irritans with similar susceptibility. in chemoattraction assays, sera and mucus collected from investigated fish ... | 2008 | 18270738 |
| efficacies of inactivated vaccines against betanodavirus in grouper larvae (epinephelus coioides) by bath immunization. | betanodavirus is the pathogen of viral nervous necrosis (vnn) disease that has caused mass mortality among many species of marine fish at larval stage. in this study, the efficacy of inactivated betanodavirus was evaluated by bath-immunization and bath-challenge of orange-spotted grouper (epinephelus coioides) at early larval stage. two kinds of chemicals were used for inactivation of the virus, and the relative percent survival (rps) values of 0.4mm binary ethylenimine (bei)-inactivated vaccine ... | 2008 | 18276044 |
| comparative studies on the immunogenicity of theronts, tomonts and trophonts of cryptocaryon irritans in grouper. | cryptocaryon irritans is a ciliated obligate parasite of marine fish, causing white spot disease in tropical and sub-tropical regions. recent studies have shown that fish can mount immune responses against c. irritans and acquire protection. the present study compared the protective immunity in grouper (epinephelus coioides) against c. irritans induced by theronts, trophonts and tomonts via intraperitoneal (i.p.) injection and tentatively identified an immobilization antigen. specific antibody t ... | 2008 | 17926065 |
| interleukin-1beta gene in orange-spotted grouper, epinephelus coioides: molecular cloning, expression, biological activities and signal transduction. | il-1beta, a key mediator of inflammation, orchestrates a variety of immune responses by initiating gene expression. herein, we have cloned and sequenced the il-1beta in orange-spotted grouper (epinephelus coioides), produced soluble mature recombinant il-1beta in escherichia coli, and characterized its biological properties and downstream signal transduction. the grouper il-1beta cdna was 1364bp in length, containing an open reading frame of 765bp. the predicted protein of 254 amino acids reveal ... | 2008 | 17920124 |
| an outer membrane protein, ompk, is an effective vaccine candidate for vibrio harveyi in orange-spotted grouper (epinephelus coioides). | the outer membrane proteins of the fish pathogen, vibrio harveyi, have a role in interaction between bacterium and host and are potential candidates for vaccine development. in this study, the gene encoding an outer membrane protein, ompk, which serves as the receptor for broad-host-range vibriophage kvp40 in v. harveyi, was isolated and characterized. then the ompk gene coding for mature peptide was subcloned into prokaryotic expression vector pbv220 and transformed into escherichia coli dh5 al ... | 2008 | 18854216 |
| dietary sodium alginate administration affects fingerling growth and resistance to streptococcus sp. and iridovirus, and juvenile non-specific immune responses of the orange-spotted grouper, epinephelus coioides. | the percent weight gain (pwg) and feeding efficiency (fe) of fingerling orange-spotted grouper, epinephelus coioides, fed diets containing sodium alginate at 1.0 and 2.0 g kg(-1) were calculated on the 2nd, 4th, 6th, and 8th weeks after feeding. survival rates of the fingerling grouper against streptococcus sp. and an iridovirus, and non-specific immune parameters such as alternative complement activity (ach50), lysozyme activity, natural haemagglutination activity, respiratory bursts, superoxid ... | 2008 | 18485740 |
| organization and promoter analysis of the grouper (epinephelus coioides) epinecidin-1 gene. | epinecidin-1, an antimicrobial peptide documented in some fish, is an essential component of the innate immune response in fish, but little is known about its gene regulation. to better understand the molecular mechanism controlling transcription of the epinecidin-1 gene, we cloned and sequenced the genes and promoter regions of three epinecidin-1 peptides from the grouper (epinephelus coioides). these genes have the potential to encode three putative epinecidin-1 peptides with either a short or ... | 2008 | 18514559 |
| prevalence of zoonotic metacercariae in two species of grouper, epinephelus coioides and epinephelus bleekeri, and flathead mullet, mugil cephalus, in vietnam. | fishborne zoonotic metacercariae have not been reported from brackish water and marine fish from vietnam waters although these parasites are common in the country's freshwater fish. both wild-caught and cultured grouper (epinephelus coioides and epinephelus bleekeri), and mullet (mugil cephalus) from brackish and marine waters located in khanh hoa province in central coastal vietnam were examined, and found positive for zoonotic trematode metacercariae. from grouper, heterophyopsis continua and ... | 2008 | 18552542 |
| molecular cloning and expression characterization of apoc-i in the orange-spotted grouper. | endogenous yolk nutrients are crucial for embryo and larval development in fish, but developmental behavior of the genes that control yolk utilization remains unknown. apolipoproteins have been shown to play important roles in lipid transport and uptake through the circulation system. in this study, ecapoc-i, the first cloned apoc-i in teleosts, has been screened from pituitary cdna library of female orange-spotted grouper (epinephelus coioides), and the deduced amino acid sequence shows 43.5% i ... | 2008 | 18958591 |
| role of alkaline serine protease, asp, in vibrio alginolyticus virulence and regulation of its expression by luxo-luxr regulatory system. | the alkaline serine protease asp, which was shown to be a virulence factor of vibrio alginolyticus as a purified protein, was cloned from v. alginolyticus epgs, a strain recently isolated from moribund epinephelus coioides in an outbreak of vibriosis in a mariculture farm of shenzhen. the asp null mutant was constructed by homologous recombination with suicide plasmid pnq705-1. compared with the wild-type strain, the asp null mutant exhibited a significant decrease of total extracellular proteas ... | 2009 | 19494689 |
| molecular cloning and functional analysis of polymeric immunoglobulin receptor gene in orange-spotted grouper (epinephelus coioides). | as one of the most important mucosal effectors, polymeric immunoglobulin receptor (pigr) mediates the transcytosis of polymeric immunoglobulins (pigs) to protect the organisms. in this study, a full-length cdna of pigr was isolated from orange-spotted grouper (epinephelus coioides), and the sequence analysis of deduced protein revealed the presence of only two ig-like domains (ilds), and the absence of the conserved ig-binding site and complementary determining region (cdr). the grouper pigr mrn ... | 2009 | 19602447 |
| survey for fishborne zoonotic metacercariae in farmed grouper in vietnam. | because of the common and growing practice of consuming raw grouper in vietnam, the potential for transmission of zoonotic parasites is unclear. an investigation of the prevalence of zoonotic parasites in cage-reared grouper (epinephelus coioides and epinephelus bleekeri) from marine waters of cat ba island, hai phong city, northern vietnam, revealed the presence of two zoonotic trematode metacercariae species, procerovum varium and heterophysopsis continua. the identity of the metacercariae was ... | 2009 | 19630515 |
| dietary administration of the probiotic, lactobacillus plantarum, enhanced the growth, innate immune responses, and disease resistance of the grouper epinephelus coioides. | the percent weight gain (pwg) and feed efficiency (fe) of epinephelus coioides were calculated, and the lactobacilli and total microbiota in the posterior intestines, and non-specific immune parameters of grouper, and its susceptibility to streptococcus sp. and an iridovirus were determined when the fish were fed diets containing lactobacillus plantarum at 0 (control), 10(6), 10(8), or 10(10) colony-forming units (cfu) kg(-1) for 4 weeks. results showed that grouper fed a diet containing l. plan ... | 2009 | 19264134 |
| effects of temperature change on the innate cellular and humoral immune responses of orange-spotted grouper epinephelus coioides and its susceptibility to vibrio alginolyticus. | orange-spotted grouper epinephelus coioides held at 27 degrees c were then further cultured at 19, 27 (control), and 35 degrees c, and were examined for innate cellular and humoral responses after 3-96 h. the total leucocyte count, respiratory burst, and phagocytic activity significantly decreased 3, 48, and 96 h after fish were transferred to 19 and 35 degrees c. both the alternative complement pathway (ach(50)) and the lysozyme activity significantly decreased at 3-96 h after fish were transfe ... | 2009 | 19332138 |
| a new approach to visualize ecosystem health by using parasites. | a new approach is chosen to visualize ecosystem health by using parasite bioindicators in segara anakan lagoon, a brackish water ecosystem at the southern java coast, indonesia. three fish species (mugil cephalus, scatophagus argus, epinephelus coioides) were collected in two different years and sampling sites and studied for ecto- and endoparasites. additional data were taken for e. coioides from two further sites in lampung bay, sumatra, and for e. fucoguttatus out of floating cages from a mar ... | 2009 | 19337753 |
| in vitro activities of three synthetic peptides derived from epinecidin-1 and an anti-lipopolysaccharide factor against propionibacterium acnes, candida albicans, and trichomonas vaginalis. | the synthetic epinecidin-1(22-42) peptide was derived from positions 22-42 of epinephelus coioides epinecidin-1. the synthetic salf(55-76) cyclic peptide (cssalf(55-76)) and salf(55-76) linear peptide (lssalf(55-76)) contained sequences from positions 55 to 76 of the penaeus monodon anti-lipopolysaccharide factor (alf), respectively. we studied the in vitro activities of epinecidin-1(22-42), cssalf(55-76), and lssalf(55-76) against propionibacterium acnes, candida albicans, and trichomonas vagin ... | 2009 | 19463737 |
| epinecidin-1 peptide induces apoptosis which enhances antitumor effects in human leukemia u937 cells. | epinecidin-1 is an antimicrobial peptide present in the grouper (epinephelus coioides). in this study, the antitumor activity of a synthetic epinecidin-1 peptide was tested. the in vitro results showed that epinecidin-1 inhibited the proliferation of human leukemia u937 cells and increased the adp/atp ratio after 24h of treatment. the dna fragmentation assay, flow cytometric assay, and caspases-3, -8, and -9 assays indicated that epinecidin-1 could induce apoptosis in u937 cells. real-time rt-pc ... | 2009 | 19720101 |
| phylogenetic analysis of betanodavirus isolates from australian finfish. | in australia, disease caused by betanodavirus has been reported in an increasing number of cultured finfish since the first report of mortalities in 1990. partial coat protein gene sequences from the t2 or t4 regions of 8 betanodaviruses from barramundi lates calcarifer, sleepy cod oxyeleotris lineolata, striped trumpeter latris lineata, barramundi cod cromileptes altivelis, australian bass macquaria novemaculata and gold-spotted rockcod epinephelus coioides from several australian states were d ... | 2009 | 20099409 |
| epinecidin-1, an antimicrobial peptide from fish (epinephelus coioides) which has an antitumor effect like lytic peptides in human fibrosarcoma cells. | epinecidin-1, a synthetic 21-mer antimicrobial peptide originally identified from grouper (epinephelus coioides), specifically exhibited high antimicrobial activities against both gram-negative and gram-positive bacteria. in the current study we report on the in vitro cytotoxicity of the peptide, an important factor before it can be considered for further applications in cancer therapy. the cytotoxicity of epinecidin-1 was investigated against several cancer cells (a549, ha59t/vgh, hela, hepg2, ... | 2009 | 19007829 |
| a set of 16 consensus primer pairs amplifying the complete mitochondrial genomes of orange-spotted grouper (epinephelus coioides) and hong kong grouper (epinephelus akaara). | groupers are of considerable economic value; however, their classification and evolutionary relationships have long been hindered by the overwhelming number of species and lack of morphological specializations. mitochondrial genome is a source of original markers that are potentially useful in the study of phylogeny and population genetics of groupers. we describe a set of 16 new primer pairs that allow pcr amplification of the entire mitochondrial genomes of orange-spotted grouper and hong kong ... | 2009 | 21564956 |
| molecular cloning and characterization analysis of immunoglobulin m heavy chain gene in european eel (anguilla anguilla). | in this study, the immunoglobulin m heavy chain gene of european eel (anguilla anguilla) was cloned and analyzed. the full-length cdna of the igm heavy chain gene (genbank accession no. ef062515) has 2089 nucleotides encoding a putative protein of 581 amino acids. the igm heavy chain was composed of leader peptide (l), variable domain (vh), ch1, ch2, hinge, ch3, ch4, and c-terminus and two novel continuous putative n-glycosylation sites were found close to the second cysteine of ch3 in a. anguil ... | 2009 | 19013650 |
| regulation of preprosomatostatin 1 (pss1) gene expression by 17beta-estradiol and identification of the pss1 promoter region in orange-spotted grouper (epinephelus coioides). | in the present paper the effects of 17beta-estradiol on the expression of the preprosomatostatin 1 (pss1) in the orange-spotted grouper hypothalamus and ovary were investigated. results from in vivo of intraperitoneal injection and in vitro static cultures showed that estradiol increased the mrna expression of pss1 gene in both hypothalamus and ovary. to investigate the molecular basis of the estrogen regulation on pss1 gene expression, we cloned the upstream region of 848bp from the translation ... | 2009 | 19559750 |
| transmission of fish parasites into grouper mariculture (serranidae: epinephelus coioides (hamilton, 1822)) in lampung bay, indonesia. | differently fed groupers epinephelus coioides from an indonesian finfish mariculture farm were studied for ecto- and endohelminth parasites. pellet-fed e. coioides were infested with 13 parasite species/taxa of which six had a monoxenous and seven a heteroxenous life cycle. a total of 14 parasite species/taxa were found in the fish that were fed with different trash fish species, four of them with a monoxenous and ten with a heteroxenous life cycle. the use of pellet food significantly reduced t ... | 2009 | 18855012 |
| immunochemical and molecular characterization of gbc4 as a tanycyte-like cell line derived from grouper brain. | a clonal cell line, gbc4, derived from grouper (epinephelus coioides) brain is proposed to represent an immature astroglial cell line because it expresses glial fibrillary acidic protein (gfap), cytokeratin and vimentin. in teleost brain, tanycytes are the most abundant gfap-expressing cell type, suggesting that gbc4 cells are derived from tanycytes. to test this hypothesis, protein and mrna expression profiles of gbc4 cells were evaluated. we detected protein and/or mrna expression of aromatase ... | 2009 | 19248839 |
| reproductive biology and implications for management of the orange-spotted grouper epinephelus coioides in the southern arabian gulf. | the reproductive biology of epinephelus coioides was determined from the examination of 1455 individuals collected between july 2005 and june 2007 in the southern arabian gulf. histological preparations of gonads indicated that males were either derived from a juvenile phase or the transition of postspawning females, confirming a diandric protogynous sexual pattern. the spawning season was well defined, occurring once a year during april and early may. peaks in spawning occurred after the full a ... | 2009 | 20735602 |
| differential profiles of gene expression in grouper epinephelus coioides, infected with singapore grouper iridovirus, revealed by suppression subtractive hybridization and dna microarray. | suppression subtractive hybridization (ssh) was used to generate a subtracted cdna library enriched with gene transcripts differentially expressed in the spleen of orange-spotted grouper epinephelus coioides after 5 days of infection with singapore grouper iridovirus (sgiv). in the forward and reverse-subtracted libraries, 260 and 153 non-redundant expressed sequence tags (est), respectively, were identified. these annotated genes responding to sgiv infection were grouped into eight gene categor ... | 2010 | 20646160 |
| growth hormone and two forms of insulin-like growth factors i in the giant grouper (epinephelus lanceolatus): molecular cloning and characterization of tissue distribution. | growth hormone and insulin-like growth factors play important roles in the growth, development and metabolism of vertebrates. in this study, we used reverse transcription and rapid amplification of cdna ends (race) to obtain the three full-length cdna sequences encoding gh and two forms of igf-i from the giant grouper (epinephelus lanceolatus), a coral fish of high commercial value cultured in southeast asia. gh precursor cdna consists of 938 bp in size with an open-reading frame (orf) encoding ... | 2010 | 20467861 |
| interferon regulatory factor-2 in orange-spotted grouper (epinephelus coioides): gene, inductive expression pattern and subcellular localization. | interferon regulatory factor 2 (irf-2) is a multifunctional transcription factor which exhibits both transcriptional activating and repressing activities in the irf family. in this study, we report an irf-2 gene isolated from orange-spotted grouper (epinephelus coioides). the 1854bp full-length cdna sequence of ecirf-2 has been cloned, encoding a putative peptide of 336 amino acids which is highly consistent with the feature of irf family members. the genomic fragment of ecirf-2 contains nine ex ... | 2010 | 19857599 |
| molecular identification of the kiss2/kiss1ra system and its potential function during 17alpha-methyltestosterone-induced sex reversal in the orange-spotted grouper, epinephelus coioides. | the kiss1/kiss1r system is a component of the hypothalamus-pituitary-gonadal (hpg) axis, which plays a crucial role in regulating gonadotropins and gonadotropin-releasing hormone. the sex reversal process is a special reproductive phenomenon regulated by the hpg axis. to better understand the neuroendocrine mechanisms of sex reversal, cdnas encoding kiss2 and kiss1ra have been cloned and functionally characterized from the orange-spotted grouper epinephelus coioides, a protogynous hermaphroditic ... | 2010 | 20375257 |
| comparative analyses of sequence structure, evolution, and expression of four somatostatin receptors in orange-spotted grouper (epinephelus coioides). | somatostatins (sss) and somatostatin receptors (sstrs) play important roles in the growth, development and metabolism of vertebrates. in the present study, four sstrs were isolated from orange-spotted grouper (epinephelus coioides), a coral fish of high commercial value cultivated in southeast asia. phylogenetic tree analysis grouped the four sstrs as two distinct groups of sstr1 and sstr2/3/5. four sstrs exhibited high homology across the vertebrates. the expression of four grouper sstr mrnas w ... | 2010 | 20347929 |