Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| description of pandoraea gen. nov. with pandoraea apista sp. nov., pandoraea pulmonicola sp. nov., pandoraea pnomenusa sp. nov., pandoraea sputorum sp. nov. and pandoraea norimbergensis comb. nov. | a polyphasic taxonomic study was performed on a group of isolates tentatively identified as burkholderia cepacia, ralstonia pickettii or ralstonia paucula (formerly known as cdc group ivc-2). the isolates were mainly cultured from sputum of cystic fibrosis patients or from soil. sds-page of whole-cell proteins and aflp fingerprinting distinguished at least five different species, and this was confirmed by dna-dna hybridizations. 16s rdna sequence analysis of representative strains indicated that ... | 2000 | 10758901 |
| phenotypic methods for determining genomovar status of the burkholderia cepacia complex. | recent taxonomic advances have demonstrated that burkholderia cepacia is a cluster of at least seven closely related genomic species (or genomovars) collectively referred to as the b. cepacia complex, all of which may cause infections among cystic fibrosis patients and other vulnerable individuals. thus, it is important for clinical microbiologists to be able to differentiate genomovars. prior to this study, 361 b. cepacia complex isolates and 51 isolates easily confused with b. cepacia complex ... | 2001 | 11230429 |
| assignment of cdc weak oxidizer group 2 (wo-2) to the genus pandoraea and characterization of three new pandoraea genomospecies. | cdc weak oxidizer group 2 (wo-2) consists of nine phenotypically similar human clinical isolates received by the centers for disease control and prevention between 1989 and 1998. four of the isolates were from blood, three were from sputum, and one each was from bronchial fluid and maxillary sinus. all are aerobic nonfermentative, motile gram-negative rods with one to eight polar flagella per cell. all grew at 25 and 35 degrees c and were positive for catalase, urease (usually delayed 3 to 7 day ... | 2001 | 11325997 |
| identification of pandoraea species by 16s ribosomal dna-based pcr assays. | the recently described genus pandoraea contains five named species (pandoraea apista, pandoraea pulmonicola, pandoraea pnomenusa, pandoraea sputorum, and pandoraea norimbergensis) and four unnamed genomospecies. pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (cf) patients. accurate genus- and species-level identification by routine clinical microbiology methods is difficult, and differentiation from burkholderia cepacia complex organisms may be especiall ... | 2001 | 11724860 |
| use of the gyrb gene for the identification of pandoraea species. | the recently described genus pandoraea consists of five named species and four unnamed genomospecies, several of which have been identified in clinical specimens including respiratory secretions from persons with cystic fibrosis. we investigated whether it is possible to distinguish species of the genus pandoraea by means of restriction fragment length polymorphism (rflp) analysis and direct sequencing of the gyrb gene. sixty-seven pandoraea isolates were included. species-specific rflp patterns ... | 2002 | 11934487 |
| biodegradation of gamma-hexachlorocyclohexane (lindane) and alpha-hexachlorocyclohexane in water and a soil slurry by a pandoraea species. | isomers of 1,2,3,4,5,6-hexachlorocyclohexane (hch) were some of the most widely used pesticides. despite reduction in their production and use, hch isomers present a serious environmental hazard. in this study, two bacterial isolates (lin-1 and lin-3) that can grow on gamma-hch as a sole source of carbon and energy were isolated from an enrichment culture. in liquid cultures of lin-1 and lin-3, 25.0 and 45.5% removal of gamma-hch, respectively, were achieved in 2 weeks. lin-3 was identified as p ... | 2002 | 11958620 |
| characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of inquilinus limosus gen. nov., sp. nov. | using a polyphasic approach (including cellular protein and fatty acid analysis, biochemical characterization, 16s ribosomal dna sequencing, and dna-dna hybridizations), we characterized 51 bacterial isolates recovered from respiratory secretions of cystic fibrosis (cf) patients. our analyses showed that 24 isolates belong to taxa that have so far not (or only rarely) been reported from cf patients. these taxa include acinetobacter sp., bordetella hinzii, burkholderia fungorum, comamonas testost ... | 2002 | 12037065 |
| temperature and ph effects on biodegradation of hexachlorocyclohexane isomers in water and a soil slurry. | this study was conducted to monitor the biodegradation of alpha-, beta-, gamma-, and delta-hexachlorocyclohexane (hch) isomers in liquid culture by a pandoraea species and determine the influence of ph and temperature on the biodegradation of alpha- and gamma-hch in liquid as well as in soil slurry cultures. the pandoraea species degraded 79.4% delta-hch and 34.3% gamma-hch in liquid culture at 4 weeks of incubation. alpha- and beta-hch exhibited almost identical rates (41.6 and 42.4%, respectiv ... | 2002 | 12188610 |
| pandoraea apista isolated from a patient with cystic fibrosis: problems associated with laboratory identification. | 2002 | 12371061 | |
| enrichment and isolation of endosulfan-degrading microorganisms. | endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,3,4-benzo-dioxathiepin-3-oxide) is a cyclodiene organochlorine currently used as an insecticide all over the world and its residues are posing a serious environmental threat. this study reports the isolation and identification of enriched microorganisms, capable of degrading endosulfan. enrichment was achieved by using the insecticide as either the sole source of carbon or sulfur in parallel studies. two strains each of f ... | 2003 | 12549541 |
| 'candidatus glomeribacter gigasporarum' gen. nov., sp. nov., an endosymbiont of arbuscular mycorrhizal fungi. | arbuscular mycorrhizal fungi are obligate endosymbionts that colonize the roots of almost 80 % of land plants. the present paper describes morphological and molecular data on a bacterial endosymbiont living in the cytoplasm of dormant or germinating spores and symbiotic mycelia of the fungal species gigaspora margarita, scutellospora persica and scutellospora castanea. pcr amplification of almost the entire 16s rrna gene of the gigaspora margarita beg 34 endosymbiont, using universal bacterial p ... | 2003 | 12656162 |
| sepsis, multiple organ failure, and death due to pandoraea pnomenusa infection after lung transplantation. | a 30-year-old man died with pandoraea pnomenusa sepsis after lung transplantation. pandoraea species are gram-negative rods, closely related to, and commonly misidentified as, burkholderia cepacia complex or ralstonia species. heretofore considered soil bacteria and colonizers that infect patients with chronic lung diseases, pandoraea species can produce severe infections. | 2003 | 12734295 |
| use of amplified ribosomal dna restriction analysis for identification of ralstonia and pandoraea species: interest in determination of the respiratory bacterial flora in patients with cystic fibrosis. | the recovery of ralstonia and pandoraea species from respiratory tract cultures of patients with cystic fibrosis has recently been reported. these species are difficult to identify, and especially to differentiate from burkholderia cepacia complex organisms, with classical methods. the discriminatory power of amplified ribosomal dna restriction analysis (ardra) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by using a panel of six en ... | 2003 | 12843108 |
| recent advances in cross-infection in cystic fibrosis: burkholderia cepacia complex, pseudomonas aeruginosa, mrsa and pandoraea spp. | 2003 | 12906328 | |
| epidemic spread of pandoraea apista, a new pathogen causing severe lung disease in cystic fibrosis patients. | we show that pandoraea apista must be added to the increasing list of pathogens capable of causing chronic lung infection in cystic fibrosis (cf) patients. it is most likely that this strain of p. apista was transmissible among patients with cf, leading to spread of infection from the index patient to 5 other patients exposed during participation in winter camps and/or hospitalization. all patients developed chronic infection with high levels of antibodies, and 4 patients had a downhill course o ... | 2003 | 14520728 |
| isolation and molecular characterization of thiosulfate-oxidizing bacteria from an italian rice field soil. | in rice paddy soils an active cycling of sulfur compounds takes place. to elucidate the diversity of thiosulfate-oxidizing bacteria these organisms were enriched from bulk soil and rice roots by the most probable number method in liquid medium. from the mpn enrichment cultures 21 bacterial strains were isolated on solid mineral medium, and could be further shown to produce sulfate from thiosulfate. these strains were characterized by 16s rdna analyses. the isolates were affiliated to seven diffe ... | 2003 | 14529188 |
| use of 16s rrna gene sequencing for rapid identification and differentiation of burkholderia pseudomallei and b. mallei. | burkholderia pseudomallei and b. mallei, the causative agents of melioidosis and glanders, respectively, are designated category b biothreat agents. current methods for identifying these organisms rely on their phenotypic characteristics and an extensive set of biochemical reactions. we evaluated the use of 16s rrna gene sequencing to rapidly identify these two species and differentiate them from each other as well as from closely related species and genera such as pandoraea spp., ralstonia spp. ... | 2003 | 14532197 |
| biodegradation kinetics of endosulfan by fusarium ventricosum and a pandoraea species. | endosulfan, classified as an organochlorine pesticide, is rated by the u.s. epa as a category 1 pesticide with extremely high acute toxicity. this study describes the biodegradation kinetics of endosulfan and the metabolic pathway utilized by fusarium ventricosum and a pandoraea sp. complete disappearance of both alpha- and beta-endosulfan was observed during 12 days of incubation with f. ventricosum in flasks containing 100 mg l(-)(1) of endosulfan. the rate constants (k) for biodegradation of ... | 2003 | 14690389 |
| burkholderia and emerging pathogens in cystic fibrosis. | persons with cystic fibrosis (cf) are susceptible to respiratory tract infection with a variety of bacterial species. among these are several species in the genus burkholderia (including members of the b. cepacia complex, b. gladioli, and b. pseudomallei), stenotrophomonas maltophilia, achromobacter ( alcaligenes) xylosoxidans, certain ralstonia species, and species within the new genus pandoraea. enterobacteriaceae and several other rather unusual or novel species also can be found. many of the ... | 2003 | 16088584 |
| pandoraea bacteremia in a cystic fibrosis patient with associated systemic illness. | pandoraea is a recently classified genus primarily isolated from the sputum of cystic fibrosis patients, but its pathogenic potential is unknown. we describe a case of pandoraea bacteremia in a 16-year-old cystic fibrosis patient associated with clinical disease, suggesting that this organism should be considered a true pathogen in susceptible patients. | 2004 | 15361734 |
| isolation of infectious cystic fibrosis patients: results of a systematic review. | respiratory tract infections significantly contribute to morbidity and mortality among cystic fibrosis (cf) patients. therefore, pathogen transmission needs to be prevented. there are several guidelines for the care of cf patients, but no transparent systematic literature review has been published. | 2005 | 15865277 |
| growth in stewart's medium is a simple, rapid and inexpensive screening tool for the identification of burkholderia cepacia complex. | ninety-one percent of burkholderia cepacia complex reference strains (66 out of 72) displayed a yellow slope-green butt colour reaction after growth in stewart's medium indicating the oxidation of glucose and the absence of an arginine dihydrolase system. this same colour reaction was observed for burkholderia gladioli and several ralstonia species, but not for pseudomonas aeruginosa, stenotrophomonas, achromobacter, pandoraea and several other gram-negative non-fermenting bacilli. we therefore ... | 2006 | 16386966 |
| chronic colonization with pandoraea apista in cystic fibrosis patients determined by repetitive-element-sequence pcr. | pandoraea apista is recovered with increasing frequency from the lungs of patients with cystic fibrosis (cf) and may represent an emerging pathogen (i. m. jorgensen et al., pediatr. pulmonol. 36:439-446, 2003). we identified two cf patients from our hospital whose sputum specimens were culture positive for p. apista over the course of several years. repetitive-element-sequence pcr was employed to determine whether sequential isolates that were recovered from these patients represented a single c ... | 2006 | 16517862 |
| differentiation of species combined into the burkholderia cepacia complex and related taxa on the basis of their fatty acid patterns. | using the established commercial system sherlock (midi, inc.), cellular fatty acid methyl ester analysis for differentiation among burkholderia cepacia complex species was proven. the identification key based on the diagnostic fatty acids is able to discern phenotypically related ralstonia pickettii and pandoraea spp. and further distinguish burkholderia pyrrocinia, burkholderia ambifaria, and burkholderia vietnamiensis. | 2006 | 16517920 |
| novel carbapenem-hydrolyzing oxacillinase oxa-62 from pandoraea pnomenusa. | pandoraea spp. are gram-negative, glucose nonfermenting rods detectable in blood cultures and sputa of cystic fibrosis patients. they are resistant to various antibiotic groups, with imipenem being the only active beta-lactam. we isolated an imipenem-resistant (mic, 64 microg/ml) pandoraea pnomenusa strain from a cystic fibrosis patient. cloning and sequencing identified two beta-lactamases of bush group 2d, namely, the known oxa-33, located on an integron, and the novel carbapenem-hydrolyzing o ... | 2006 | 16569848 |
| regioselective carboxylation of 1,3-dihydroxybenzene by 2,6-dihydroxybenzoate decarboxylase of pandoraea sp. 12b-2. | we found a bacterium, pandoraea sp. 12b-2, of which whole cells catalyzed not only the decarboxylation of 2,6-dihydroxybenzoate but also the regioselective carboxylation of 1,3-dihydroxybenzene to 2,6-dihydroxybenzoate. the whole cells of pandoraea sp. 12b-2 also catalyzed the regioselective carboxylation of phenol and 1,2-dihydroxybenzene to 4-hydroxybenzoate and 2,3-dihydroxybenzoate, respectively. the molar conversion ratio of the carboxylation reaction depended on the concentration of khco(3 ... | 2006 | 16683134 |
| [pandoraea pulmonicola isolation in patients with cystic fibrosis]. | 2006 | 16956543 | |
| occurrence of burkholderia cepacia complex, ralstonia and pandoraea species dnas in the coastal environment of the straits of messina (italy). | 2007 | 17360005 | |
| bioaugmentation and coexistence of two functionally similar bacterial strains in aerobic granules. | the survival of the inoculated microbial culture is critical for successful bioaugmentation but impossible to predict precisely. as an alternative strategy, bioaugmentation of a group of microorganisms may improve reliability of bioaugmentation. this study evaluated simultaneous bioaugmentation of two functionally similar bacterial strains in aerobic granules. the two strains, pandoraea sp. pg-01 and rhodococcus erythropolis pg-03, showed high phenol degradation and growth rates in phenol medium ... | 2007 | 17370069 |
| characterization of biphenyl dioxygenase of pandoraea pnomenusa b-356 as a potent polychlorinated biphenyl-degrading enzyme. | biphenyl dioxygenase (bpdo) catalyzes the aerobic transformation of biphenyl and various polychlorinated biphenyls (pcbs). in three different assays, bpdo(b356) from pandoraea pnomenusa b-356 was a more potent pcb-degrading enzyme than bpdo(lb400) from burkholderia xenovorans lb400 (75% amino acid sequence identity), transforming nine congeners in the following order of preference: 2,3',4-trichloro approximately 2,3,4'-trichloro > 3,3'-dichloro > 2,4,4'-trichloro > 4,4'-dichloro approximately 2, ... | 2007 | 17526697 |
| emerging and unusual gram-negative infections in cystic fibrosis. | people with cystic fibrosis (cf) have chronic airway infection and frequent exposure to antibiotics, which often leads to the emergence of resistant organisms. in addition to the development of multiresistance in common cf pathogens such as pseudomonas aeruginosa, several newer, inherently resistant gram-negative species are becoming more common, including burkholderia cepacia complex, stenotrophomonas maltophilia, achromobacter (alcaligenes) xylosoxidans, certain ralstonia species, and those wi ... | 2007 | 17562501 |
| [pandoraea cepa]. | 2007 | 17989848 | |
| induction of oxalate decarboxylase by oxalate in a newly isolated pandoraea sp. oxj-11 and its ability to protect against sclerotinia sclerotiorum infection. | pandoraea sp. oxj-11 has been shown to produce an oxalate decarboxylase. the enzyme could be induced by increasing the oxalate in the medium. an increasing concentration of yeast extract was able to stimulate the cell growth but could not increase the specific oxalate decarboxylase activity. the oxalate decarboxylase was produced maximally at 25-35 degrees c and ph 4.0-9.0, favoring its potential application in protection of host plants from oxalate-producing phytopathogens. the influence of glu ... | 2007 | 18059564 |
| isolation and some properties of newly isolated oxalate-degrading pandoraea sp. oxj-11 from soil. | to isolate and characterize an oxalate-degrading pandoraea sp. oxj-11. | 2007 | 17897211 |
| degradation of reactive dyes by ozonation and oxalic acid-assimilating bacteria isolated from soil. | ozonation and treatment of wastewaters with oxalic acid-assimilating bacterium was attempted for the complete degradation of reactive dyes. oxalic acid-assimilating bacterium, pandoraea sp. strain ebr-01, was newly isolated from soil under bamboo grove and was identified to be a member of the genus pandoraea by physicochemical and biochemical tests including 16s rdna sequence analysis. the bacterium was grown optimally at ph 7 and temperature of 30 degrees c under the laboratory conditions. reac ... | 2008 | 17926132 |
| evaluation of in vitro virulence characteristics of the genus pandoraea in lung epithelial cells. | pandoraea species are emerging opportunistic pathogens capable of causing chronic lung infections in cystic fibrosis patients. this study examined the interactions of 17 pandoraea isolates from the five identified species (pandoraea apista, pandoraea norimbergensis, pandoraea pulmonicula, pandoraea sputorum and pandoraea pnomenusa) plus two pandoraea genomospecies isolates with lung epithelial cells and their ability to form biofilms in vitro. only three isolates showed an ability to invade a549 ... | 2008 | 18065662 |
| cellular fatty acid-deficient pandoraea isolated from a patient with cystic fibrosis. | 2008 | 18201997 | |
| assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches. | microbial community structure of two distinct effluent treatment plants (etps) of pesticide and pharmaceutical industries was assessed and defined by (i) culture dependent and culture independent approaches on the basis of 16s rrna gene sequencing, (ii) diversity index analysis - operational taxonomic units (otus). a total of 38 and 44 bacterial otus having 85-99% similarity with the closest match in the database were detected among pharmaceutical and pesticide sludge samples, respectively. fift ... | 2008 | 18280146 |
| evidence of species succession during chlorobenzene biodegradation. | we have previously reported the disappearance of a specific strain degrading chlorobenzene from a functionally stable bioreactor. in the present work, we investigated this species succession and isolated a new dominant strain, identified as pandoraea pnomenusa sp. strain mcb032. a specific 16s rrna-targeted oligonucleotide probe was designed and validated to identify strain mcb032 using fluorescence in situ hybridisation (fish). the results confirmed the presence of strain mcb032 in samples coll ... | 2008 | 17680678 |
| misidentification of pandoraea sputorum isolated from sputum of a patient with cystic fibrosis and review of pandoraea species infections in transplant patients. | pandoraea species are considered emerging pathogens in cystic fibrosis (cf) patients, but few data exist regarding outcomes of patients colonized with these organisms. we report a case of pandoraea sputorum colonization in a cf patient under consideration for lung transplantation and review five cases of lung transplantation involving pandoraea species. | 2008 | 18650348 |
| potential plant growth promoting traits and bioacidulation of rock phosphate by thiosulfate oxidizing bacteria isolated from crop plants. | thiosulfate oxidizing bacteria isolated from crop plants were tested for their traits related to plant growth promotion and their ability to solubilize morocco rock phosphate (rp) through oxidation of thiosulfate to sulfuric acid. all the tested strains grew in nfb medium (except dyella ginsengisoli) and possessed beta-1,3 glucanase activity (except burkholderia kururiensis). of the fourteen tested strains, 2 were found positive for siderophore production, 3 each for phytohormones (iaa), and sal ... | 2008 | 18785656 |
| chemolithoautotrophic oxidation of thiosulfate and phylogenetic distribution of sulfur oxidation gene (soxb) in rhizobacteria isolated from crop plants. | twenty-one thiosulfate-oxidizing bacteria were isolated from rhizosphere soils and 16s rrna analysis revealed that the isolates were affiliated with seven different phylogenetic groups within the beta and gamma subclasses of proteobacteria and actinobacteria. among these, five genera, including dyella, burkholderia, alcaligenes, microbacterium and leifsonia sp., represented new sulfur oxidizers in rhizosphere soils. the thiosulfate-oxidizing dyella, burkholderia, alcaligenes, microbacterium, lei ... | 2008 | 18832027 |
| in vitro activities of a novel nanoemulsion against burkholderia and other multidrug-resistant cystic fibrosis-associated bacterial species. | respiratory tract infection, most often involving opportunistic bacterial species with broad-spectrum antibiotic resistance, is the primary cause of death in persons with cystic fibrosis (cf). species within the burkholderia cepacia complex are especially problematic in this patient population. we investigated a novel surfactant-stabilized oil-in-water nanoemulsion (nb-401) for activity against 150 bacterial isolates recovered primarily from cf respiratory tract specimens. these specimens includ ... | 2009 | 18955531 |
| viable ultramicrocells in drinking water. | to examine the diversity of cultivable 0.2 micron filtrate biofilm forming bacteria from drinking water systems. | 2009 | 19040704 |
| genetic and biochemical analyses of chlorobenzene degradation gene clusters in pandoraea sp. strain mcb032. | pandoraea sp. strain mcb032 was isolated as an emerging chlorobenzene degrader from a functionally stable bioreactor where species succession had occurred. in this study, two gene clusters encoding chlorobenzene metabolic functions have been cloned. within the cbs gene cluster, cbsa and cbsb are similar to the chlorobenzene dioxygenase and the cis-chlorobenzene dihydrodiol dehydrogenase in ralstonia sp. js705 and shown to transform chlorobenzene to 3-chlorocatechol. the clc gene cluster shows st ... | 2009 | 19365620 |
| 16s rrna gene-based phylogenetic microarray for simultaneous identification of members of the genus burkholderia. | for cultivation-independent and highly parallel analysis of members of the genus burkholderia, an oligonucleotide microarray (phylochip) consisting of 131 hierarchically nested 16s rrna gene-targeted oligonucleotide probes was developed. a novel primer pair was designed for selective amplification of a 1.3 kb 16s rrna gene fragment of burkholderia species prior to microarray analysis. the diagnostic performance of the microarray for identification and differentiation of burkholderia species was ... | 2009 | 19396938 |
| proteolytic activity in stored aerobic granular sludge and structural integrity. | aerobic granules lose stability during storage. the goal of this work was to highlight the main cause of stability loss for stored granules as intracellular protein hydrolysis. the quantity of extracellular proteins was noted to be significantly lower during granule storage, and protease enzyme activities were correspondingly higher in the cores of stored granules. the proteolytic bacteria, which secrete highly active protease enzymes, were for the first time isolated and characterized by analyz ... | 2009 | 18614356 |
| biological decolorization of dye solution containing malachite green by pandoraea pulmonicola yc32 using a batch and continuous system. | in our study, we have isolated a relatively newly identified bacteria species, pandoraea pulmonicola yc32, and first assessed its capability to treat malachite green (mg). the effects of various factors on decolorization efficiency were investigated in a batch system. the decolorization efficiency was found to be optimal within a ph of 7-10 and it increased, with increasing initial mg concentration up to 100 mg/l. the relationship between the decolorization rate and mg concentration agreed with ... | 2009 | 19717235 |
| eurocarecf quality assessment of diagnostic microbiology of cystic fibrosis isolates. | the identification of microbial species from respiratory specimens and their susceptibility to antimicrobial agents are among the most important diagnostic measures of care for patients with cystic fibrosis (cf). under the umbrella of eurocarecf, two quality assurance trials of cf microbiology were performed in 2007 and 2008. nine formulations with cf bacterial isolates were dispatched. a total of 31/37 laboratories from 18/21 european countries participated in the 2007 and 2008 trials. the comm ... | 2009 | 19741077 |
| enhanced degradation of phenanthrene in a solid-liquid two-phase partitioning bioreactor via sonication. | the current article examined the feasibility of inducing improved delivery and degradation of phenanthrene in a solid-liquid partitioning bioreactor system at bench scale by means of ultrasonic energy input. initial degradation rates of phenanthrene by a microbial consortium, delivered from desmopan, were improved 2.7-fold in the presence of sonication relative to unsonicated controls. results demonstrated that an operating window involving on/off sonication cycling improved substrate delivery a ... | 2010 | 19998286 |
| polyphasic analysis of the bacterial community in the rhizosphere and roots of cyperus rotundus l. grown in a petroleum-contaminated soil. | cyperus rotundus l. is a perennial herb which was found prevailing in an area in the northeast of brazil previously contaminated with petroleum. in order to enlarge the knowledge of microorganism-plant interaction in phytoremediation, the bacterial community present in the rhizosphere and roots of c. rotundus was evaluated by culture-dependent and molecular approaches. pcr-dgge analyses based on the 16s rrna gene showed that the profiles of bulk soil, rhizosphere and root samples had a high degr ... | 2010 | 20519908 |
| construction of an artificial microalgal-bacterial consortium that efficiently degrades crude oil. | four oil component-degrading bacteria and one oil-tolerant microalgae, scenedesmus obliquus gh2, were used to construct an artificial microalgal-bacterial consortium for crude-oil degradation. the bacterial strains included sphingomonas gy2b and burkholderia cepacia gs3c, along with a mixed culture, named gp3, containing pseudomonas gp3a and pandoraea pnomenusa gp3b. gy2b could only degrade polycyclic aromatic hydrocarbons, gs3c was able to degrade aliphatic chain hydrocarbons, and gp3 could uti ... | 2010 | 20638971 |
| pandoraea oxalativorans sp. nov., pandoraea faecigallinarum sp. nov. and pandoraea vervacti sp. nov., isolated from oxalate-enriched culture. | five isolates, ta2, ta4, ta25(t), kox(t) and ns15(t) which were isolated in previous studies by enrichment in mineral medium with potassium oxalate as the sole carbon source were characterized using a polyphasic approach. the isolates were gram-negative, aerobic, non-spore-forming rods. phylogenetic analysis based on 16s rrna and dna gyrase b subunit (gyrb) gene nucleotide sequences confirmed that the isolates belonged to the genus pandoraea, most closely related to pandoraea sputorum and pandor ... | 2010 | 20952546 |
| expression, purification, crystallization and preliminary crystallographic studies of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from pandoraea pnomenusa b-356. | cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase (bphb) is involved in the aerobic biodegradation of biphenyl and polychlorinated biphenyls. bphb from pandoraea pnomenusa strain b-356 was overexpressed in escherichia coli, purified to homogeneity and crystallized. crystals were obtained by the sitting-drop vapour-diffusion method using polyethylene glycol 3350 and 0.2 m sodium malonate. a bphb crystal diffracted to 2.8 å resolution and belonged to space group p4(3)2(1)2, with unit-cell parameters ... | 2010 | 21045310 |
| virulence of an emerging respiratory pathogen, genus pandoraea, in vivo and its interactions with lung epithelial cells. | pandoraea species have emerged as opportunistic pathogens among cystic fibrosis (cf) and non-cf patients. pandoraea pulmonicola is the predominant pandoraea species among irish cf patients. the objective of this study was to investigate the pathogenicity and potential mechanisms of virulence of irish p. pulmonicola isolates and strains from other pandoraea species. three patients from whom the p. pulmonicola isolates were isolated have since died. the in vivo virulence of these and other pandora ... | 2010 | 21127160 |
| pandoraea thiooxydans sp. nov., a facultatively chemolithotrophic, thiosulfate-oxidizing bacterium isolated from rhizosphere soils of sesame (sesamum indicum l.). | a facultatively chemolithoautotrophic, thiosulfate-oxidizing, gram-negative, aerobic, motile, rod-shaped bacterial strain, designated atsb16(t), was isolated from rhizosphere soils of sesame (sesamum indicum l.). 16s rrna gene sequence analysis demonstrated that this strain was closely related to pandoraea pnomenusa lmg 18087(t) (96.7 % similarity), p. pulmonicola lmg 18016(t) (96.5 %), p. apista lmg 16407(t) (96.2 %), p. norimbergensis lmg 18379(t) (96.1 %) and p. sputorum lmg 18819(t) (96.0 %) ... | 2010 | 19643869 |
| Clinical and microbiological features of a cystic fibrosis patient chronically colonized with Pandoraea sputorum identified combining 16S rRNA sequencing and MALDI-TOF mass spectrometry. | Clonal isolates identified as various non-fermentative Gram-negative bacilli over a 5-year period from sputum cultures of a 30-year old cystic fibrosis patient were successfully re-identified as Pandoraea sputorum by combining 16S rRNA sequencing and MALDI-TOF MS. A decreased lung function improved after one-year of azithromycin and inhaled 7%-hypertonic saline treatment. | 2011 | 22170922 |
| maldi-tof ms improves routine identification of non-fermenting gram negative isolates from cystic fibrosis patients. | identification of non-fermenting gram-negative bacteria (nfgnb) from cystic fibrosis (cf) patients is often limited. a collection of stored nfgnb isolates (n=182) recovered from cf patients over a 15year period was examined. the routinely reported identification during this period was compared with that obtained by maldi-tof ms. isolates giving discrepant identification at the genus level were further analyzed by 16s rdna sequencing. the maldi-tof ms system identified 94% of the isolates, includ ... | 2011 | 21968086 |
| biochemical studies and ligand-bound structures of biphenyl dehydrogenase from pandoraea pnomenusa strain b-356 reveal a basis for broad specificity of the enzyme. | biphenyl dehydrogenase, a member of short-chain dehydrogenase/reductase enzymes, catalyzes the second step of the biphenyl/polychlorinated biphenyls catabolic pathway in bacteria. to understand the molecular basis for the broad substrate specificity of pandoraea pnomenusa strain b-356 biphenyl dehydrogenase (bphb(b-356)), the crystal structures of the apo-enzyme, the binary complex with nad(+), and the ternary complexes with nad(+)-2,3-dihydroxybiphenyl and nad(+)-4,4'-dihydroxybiphenyl were det ... | 2011 | 21880718 |
| insight into the metabolism of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (ddt) by biphenyl dioxygenases. | in this work we have investigated the ability of the biphenyl dioxygenase of burkholderia xenovorans lb400 (bphae(lb400)) and of pandoraea pnomenusa b356 (bphae(b356)) to metabolize ddt. data show bphae(lb400) is unable to metabolize this substrate but bphae(b356) metabolizes ddt to produce two stereoisomers. structural analysis of ddt-docked bphae(lb400) and bphae(b356) identified residue phe336 of bphae(lb400) as critical to prevent productive binding of ddt to bphae(lb400). furthermore, the f ... | 2011 | 22001737 |
| 2,4,5-trichlorophenol degradation using a novel tio2-coated biofilm carrier: roles of adsorption, photocatalysis, and biodegradation. | intimate coupling of photocatalysis and biodegradation (icpb) offers potential for degrading biorecalcitrant and toxic organic compounds. this study reports on a novel sponge-type, tio(2)-coated biofilm carrier that showed significant adherence of tio(2) and ability to accumulate biomass in its interior. this carrier was tested for icpb in a continuous-flow photocatalytic circulating-bed biofilm reactor (pcbbr) to mineralize 2,4,5-trichlorophenol (tcp), which is biorecalcitrant. four mechanisms ... | 2011 | 21861477 |
| [pandoraea sputorum colonization in a patient with cystic fibrosis]. | cystic fibrosis (cf) is a disease produced by a defect in the transmembrane conductance regulator protein, cftr. currently, the morbidity and mortality associated with cf are fundamentally related with the lung affectation that is a consequence of this defect. with the progression of the disease, there is an increase in the isolation of non-fermenting gram-negative bacilli colonizing these patients. the genus pandoraea arises from a reclassification of species included within the "burkholderia c ... | 2011 | 21908092 |
| isolation and characterization of novel bacterial strains exhibiting ligninolytic potential. | to expand on the range of products which can be obtained from lignocellulosic biomass, the lignin component should be utilized as feedstock for value-added chemicals such as substituted aromatics, instead of being incinerated for heat and energy. enzymes could provide an effective means for lignin depolymerization into products of interest. in this study, soil bacteria were isolated by enrichment on kraft lignin and evaluated for their ligninolytic potential as a source of novel enzymes for wast ... | 2011 | 21995752 |