Publications

methanomicrococcus blatticola gen. nov., sp. nov., a methanol- and methylamine-reducing methanogen from the hindgut of the cockroach periplaneta americana.	a small irregular coccoid methanogenic bacterium (pat) was isolated from the hindgut of the cockroach periplaneta americana. fluorescence microscopy and transmission electron microscopy of the hindgut of p. americana suggest that the organism occurs abundantly in the microbiota attached to the hindgut wall. the strain produces methane by the reduction of methanol and methylated amines with molecular hydrogen. acetate, coenzyme m, yeast extract, tryptic soy broth and vitamins are required for gro ...	2000	11155972
comparison of euryarchaea strains in the guts and food-soil of the soil-feeding termite cubitermes fungifaber across different soil types.	termites are an important component of tropical soil communities and have a significant effect on the structure and nutrient content of soil. digestion in termites is related to gut structure, gut physicochemical conditions, and gut symbiotic microbiota. here we describe the use of 16s rrna gene sequencing and terminal-restriction fragment length polymorphism (t-rflp) analysis to examine methanogenic archaea (ma) in the guts and food-soil of the soil-feeder cubitermes fungifaber sjostedt across ...	2004	15240259
the energy metabolism of methanomicrococcus blatticola: physiological and biochemical aspects.	methanomicrococcus blatticola, a methanogenic archaeon isolated from the cockroach periplaneta americana, is specialised in methane formation by the hydrogen-dependent reduction of methanol, monomethyl-, dimethyl- or trimethylamine. experiments with resting cells demonstrated that the capability to utilise the methylated one-carbon compounds was growth substrate dependent. methanol-grown cells were incapable of methylamine conversion, while cells cultured on one of the methylated amines did not ...	2005	15928982
the competitive success of methanomicrococcus blatticola, a dominant methylotrophic methanogen in the cockroach hindgut, is supported by high substrate affinities and favorable thermodynamics.	methanomicrococcus blatticola is an obligately anaerobic methanogen that derives the energy for growth exclusively from the reduction of methylated compounds to methane with molecular hydrogen as energy source. competition for methanol (concentration below 10 microm) and h(2) (concentration below 500 pa), as well as oxidative stress due to the presence of oxygen are likely to occur in the peripheral region of the cockroach hindgut, the species' normal habitat. we investigated the ecophysiologica ...	2007	17367516
analysis of methanogen diversity in the rumen using temporal temperature gradient gel electrophoresis: identification of uncultured methanogens.	a temporal temperature gradient gel electrophoresis (ttge) method was developed to determine the diversity of methanogen populations in the rumen. tests with amplicons from genomic dna from 12 cultured methanogens showed single bands for all strains, with only two showing apparently comigrating bands. fingerprints of methanogen populations were analyzed from dna extracted from rumen contents from two cattle and four sheep grazing pasture. for one sheep, dilution cultures selective for methanogen ...	2007	17431710
diversity of the resident microbiota in a thermophilic municipal biogas plant.	biogas plants continuously convert biological wastes mainly into a mixture of methane, co2 and h2o-a conversion that is carried out by a consortium of bacteria and archaea. especially in the municipal plants dedicated towards waste treatment, the reactor feed may vary considerably, exposing the resident microbiota to a changing variety of substrates. to evaluate how and if such changes influence the microbiology, an established biogas plant (6,600 m3, up to 600 m3 biogas per h) was followed over ...	2008	18820906