Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| evolution of wild cereals during 28 years of global warming in israel. | climate change is a major environmental stress threatening biodiversity and human civilization. the best hope to secure staple food for humans and animal feed by future crop improvement depends on wild progenitors. we examined 10 wild emmer wheat (triticum dicoccoides koern.) populations and 10 wild barley (hordeum spontaneum k. koch) populations in israel, sampling them in 1980 and again in 2008, and performed phenotypic and genotypic analyses on the collected samples. we witnessed the profound ... | 0 | 22334646 |
| high-density genetic map of durum wheat x wild emmer wheat based on ssr and dart markers. | a genetic linkage map of tetraploid wheat was constructed based on a cross between durum wheat [triticum turgidum ssp. durum (desf.) mackey] cultivar langdon and wild emmer wheat [t. turgidum ssp. dicoccoides (körn.) thell.] accession g18-16. one hundred and fifty-two single-seed descent derived f(6) recombinant inbred lines (rils) were analyzed with a total of 690 loci, including 197 microsatellite and 493 dart markers. linkage analysis defined 14 linkage groups. most markers were mapped to the ... | 0 | 18437346 |
| comparative rflp mapping of the chlorotoluron resistance gene (su1) in cultivated wheat (triticum aestivum) and wild wheat (triticum dicoccoides). | chlorotoluron is a selective phenylurea herbicide widely used for broad-leaved and annual grass weed control in cereals. variation in the response to chlorotoluron (ct) was found in both hexaploid bread wheat (triticum aestivum l.) and wild tetraploid wheat (triticum dicoccoides körn.). here, we describe the comparative mapping of the ct resistance gene (su1) on chromosome 6b in bread and wild wheat using rflp markers. in bread wheat, mapping was based on 58 f(4) single-seed descent (ssd) plants ... | 0 | 19352744 |
| genetic divergence of heat production within and between the wild progenitors of wheat and barley: evolutionary and agronomical implications. | we compared and contrasted calorimetrically heat production in seedlings incubated at 5°c and 24°c using genotypes from cold and warm israeli populations of the wild progenitors of barley (hordeum spontaneum) and wheat (triticum dicoccoides). the wild barley sample comprised 14 accessions, 7 from cold localities and 7 from warm localities. the wild emmer wheat sample consisted of 12 accessions, 6 from a cold locality, and 6 from a warm locality. our results indicated that (1) heat production was ... | 0 | 24201500 |
| assessment of genomic and species relationships in triticum and aegilops by page and by differential staining of seed albumins and globulins. | endosperm protein components from common bread wheats (triticum aestivum l.) and related species were extracted with aluminum lactate, ph 3.2, and examined by electrophoresis in the same buffer. electrophoretic patterns of the albumins and globulins were compared to evaluate the possibility that a particular species might have contributed its genome to tetraploid or hexaploid wheat. together with protein component mobilities, differential band staining with coomassie brilliant blue r250 was empl ... | 1978 | 24317663 |
| genetic diversity and environmental associations of wild wheat, triticum dicoccoides, in israel. | allozyme variation in the tetraploid wild progenitor of wheat, triticum dicoccoides, was studied for the proteins encoded by about 50 gene loci in 457 individuals representing 12 populations from israel. six spikelet morphological traits were measured in the same populations. the results indicate that: (a) 16 loci (= 32%) were monomorphic in all 12 populations, 15 loci (= 30%) were locally polymorphic, and 19 loci (= 38%) were regionally polymorphic. all polymorphic loci (but one) displayed high ... | 1982 | 24270617 |
| a computer-assisted examination of the storage protein genetic variation in 841 accessions of triticum dicoccoides. | triticum turgidum l. var. dicoccoides (wild emmer) is an important genetic resource for increasing the protein content of common wheat (triticum aestivum l.). many studies have shown that the presence or absence of bands in sodium dodecyl sulfate polyacrylamide (sds-page) electrophoregrams of wheat storage proteins to be of a purely genetic character. a total protein extraction and sds-page technique was used to estimate the storage protein genetic variability among 841 accessions of wild emmer ... | 1984 | 24253627 |
| linkage relationships in wild emmer wheat, triticum dicoccoides. | the linkage relationships in wild emmer wheat, triticum dicoccoides , between nine enzymatic loci (mdh-1, ipo, beta-glu, pept-1, pept-3, est-5, est-1, 6pgdh-2 and hk) and a coleoptile pigment locus (rc) were investigated. chromosome locations of genes were inferred from analysis of ditelocentric lines of triticum aestivum, cultivar chinese spring. the loci mdh-b1 and hk are linked (lambda = 0.1869) and are most likely located on the chromosome 1b. the loci pept-b1 and rc are linked (lambda = 0.2 ... | 1986 | 17246352 |
| quantitative variation in the kernel proteins among 841 accessions of triticum dicoccoides estimated by sds-page. | the relative proportion and amount of proteins in five defined molecular weight (mw) regions (a1=above 71,000=71k, a2=71k-49k, a3=49k-31k, a4=31k-20k, a5=20k and less) were estimated by densitometric analyses of the amount of dye bound by kernel proteins (fullington et al. 1980) of triticum dicoccoides sds-page gels. these mw regions roughly correspond to the wheat protein solubility classes (cole et al. 1981; fullington et al. 1983). one purpose of the study was to select accessions whose seed ... | 1986 | 24247934 |
| estimation of gene flow and genetic neighborhood size by indirect methods in a selfing annual, triticum dicoccoides. | rates of gene flow and neighborhood area were investigated in the selfing annual, triticum dicoccoides. the collection of seed material was made using a spatial hierarchical sampling design in which four collection sites were 5-7 m apart, four were 15-17 m apart, and four were 100-120 m apart. between 25 and 30 plants were scored from each site (mean sample size = 28.7). two procedures for indirect estimation of gene flow were used on gene-frequency data from 13 polymorphic loci. the estimates o ... | 1987 | 28563596 |
| wheat storage proteins: diversity of hmw glutenin subunits in wild emmer from israel : 1. geographical patterns and ecological predictability. | the diversity of hmw glutenin subunits in the tetraploid wild progenitor of wheat, triticum turgidum var. dicoccoides was studied electrophoretically in 231 individuals representing 11 populations of wild emmer from israel. the results show that (a) the two hmw glutenin loci, glu-a1 and glu-b1, are rich in variation, having 11 and 15 alleles, respectively, (b) genetic variation in hmw glutenin subunits is often severely restricted in individual populations, supporting an island population geneti ... | 1987 | 24240347 |
| natural selection of allozyme polymorphisms: a microgeographical differentiation by edaphic, topographical, and temporal factors in wild emmer wheat (triticum dicoccoides). | allozymic variation in proteins encoded by 47 loci was analyzed electrophoretically in 1983/4 and 1984/5 in 356 individual plants of wild emmer wheat, triticum dicoccoides, from a microsite at tabigha, north of the sea of galilee, israel. each year the test involved two 100-meter transects, each equally subdivided into basalt and terra rossa soil types, and comparisons were based on 16 common polymorphic loci. significant genetic differentiation, genetic phase disequilibria, and genome organizat ... | 1988 | 24232353 |
| migration patterns and the development of multilocus associations in a selfing annual, triticum dicoccoides. | migration patterns in wild emmer wheat, triticum dicoccoides, were inferred from single-locus and multilocus genetic distributions based primarily on expectations derived from single-locus and multilocus cline theory. populations from five collections displayed a large amount of multilocus structuring, as indicated by high values of gametic-phase disequilibrium between pairs of loci and by high values of multilocus associations. analyses of the distributions of individual alleles, however, indic ... | 1989 | 28568391 |
| genetic diversity of wild emmer wheat in israel and turkey : structure, evolution, and application in breeding. | allozyme variation in the tetraploid wild emmer wheat, triticum dicoccoides, the progenitor of all cultivated wheats, was studied for the proteins encoded by 42 gene loci in 1815 plants representing 37 populations - 33 from israel and 4 from turkey - sampled in 33 localities from 1979 to 1987. the results showed that: (a) 6 loci (14%) were monomorphic in all populations, 15 loci (36%) were locally polymorphic, and 21 loci (50%) were regionally polymorphic. these results are similar to those obta ... | 1989 | 24232622 |
| genetic diversity of photosynthetic characters in native populations of triticum dicoccoides. | wild emmer wheat (triticum dicoccoides korn) has shown wide genetic diversity for disease resistance and morpho-physiological traits of economic importance. our objectives were to test for genetic variation (vg) in photosynthetic characteristics residing within and between native populations sampled from three ecogeographical regions of israel, and to identify potential sources of high photosynthetic efficiency for future wheat improvement. accessions sampled in the center of wild emmer distribu ... | 1990 | 24420278 |
| the molecular basis of genetic diversity among cytoplasms of triticum and aegilops : 7. restriction endonuclease analysis of mitochondrial dnas from polyploid wheats and their ancestral species. | many related species and strains of common wheat were compared by matching differences among their mitochondrial genomes with their "parent" nuclear genomes. we examined three species of aegilops, section sitopsis (ae. bicornis, ae. sharonensis, and ae. speltoides), emmer wheat (triticum dicoccoides, t. dicoccum, and t. durum), common wheat (t. spelta, t. aestivum, and t. compaction), and timopheevi wheat (t. araraticum, t. timopheevi, and t. zhukovskyi). a single source of the cytoplasm was use ... | 1990 | 24220971 |
| photosynthetic performance in wild emmer wheat, triticum dicoccoides: ecological and genetic predictability. | in a twin study, we have shown that wild emmer wheat, triticum dicoccoides, the progenitor of all cultivated wheats, harbours important genetic variation (vg) in photosynthetic characteristics. this vg resides within and between populations and ecogeographical regions in israel, which is the center of origin and diversity of wild emmer wheat. here we analyzed, by univariate and multivariate methods, the significant differentiation of variation in photosynthetic characteristics of 107 genotypes f ... | 1991 | 24221309 |
| herbicide response polymorphisms in wild emmer wheat: ecological and isozyme correlations. | we demonstrate that the scores and frequencies of chlortoluron (ct) and metoxuron (mx) resistance and susceptible phenotypes of wild emmer wheat, triticum dicoccoides, are correlated with ecological factors and allozyme markers. some isozyme markers located on chromosome 6b (e.g. adh,est-4 and got), which also harbours the ct and mx resistance gene, provide good genetic markers for herbicide resistance breeding. significant correlations between herbicide and photosynthetic characters suggest tha ... | 1992 | 24203049 |
| storage-protein variation in wild emmer wheat (triticum turgidum ssp.dicoccoides) from jordan and turkey. i. electrophoretic characterization of genotypes. | seed storage-protein variation at theglu-a1,glu-b1 andgli-b1/glu-b3 loci in the tetraploid wild progenitor of wheat,t. dicoccoides, was studied electrophoretically in 315 individuals representing nine populations from jordan and three from turkey. a total of 44 different hmw-glutenin patterns were identified, resulting from the combination of 15 alleles in the a genome and 19 in the b genome. twenty-seven new allelic variants, 12 at theglu-a1 locus and 15 at theglu-b1 locus, were identified by c ... | 1993 | 24193595 |
| genetic polymorphisms of α- and β-amylase isozymes in wild emmer wheat, triticum dicoccoides, in israel. | α- and β-amylase isozyme diversity was studied electrophoretically by thin-layer polyacrylamide gel isoelectrofocusing in the tetraploid wild emmer wheat, triticum dicoccoides, the progenitor of all cultivated wheats. we analyzed 225 plants from 23 populations encompassing the ecological spectrum of t. dicoccoides in israel. the results were as follows: (a) band and multilocus genotype polymorphisms abound and vary within and between the four amylase components: malt, green (α-amylases), and dry ... | 1993 | 24196155 |
| application of the random amplified polymorphic dna technique for the detection of polymorphism among wild and cultivated tetraploid wheats. | development of a high-density genetic linkage map of cultivated wheats using conventional molecular markers has lagged behind the other major food crops such as rice and tomato because of the large genome size and limited levels of genetic polymorphisms. recently, random amplified polymorphic dnas (rapds) have been suggested to provide an alternative to visualize more polymorphism. for the construction of a genetic linkage map in tetraploid wheats, one can use a strategy of intersubspecific cros ... | 1993 | 8349131 |
| genetic studies of leaf and stem rust resistance in six accessions of triticum turgidum var. dicoccoides. | six accessions of triticum turgidum var. dicoccoides l. (4x, aabb) of diverse origin were tested with 10 races of leaf rust (puccinia recondita f.sp. tritici rob. ex desm.) and 10 races of stem rust (p. graminis f.sp. tritici eriks. &henn.). their infection type patterns were all different from those of lines carrying the lr or sr genes on the a or b genome chromosomes with the same races. the unique reaction patterns are probably controlled by genes for leaf rust or stem rust resistance that ha ... | 1994 | 18470084 |
| molecular variation in chloroplast dna regions in ancestral species of wheat. | restriction map variation in two 5-6-kb chloroplast dna regions of five diploid aegilops species in the section sitopsis and two wild tetraploid wheats, triticum dicoccoides and triticum araraticum, was investigated with a battery of four-cutter restriction enzymes. a single accession each of triticum durum, triticum timopheevi and triticum aestivum was included as a reference. more than 250 restriction sites were scored, of which only seven sites were found polymorphic in aegilops speltoides. n ... | 1994 | 7916310 |
| chromosome translocations in wild populations of tetraploid emmer wheat in israel and turkey. | translocation frequencies (as compared to the standard chromosome arrangement typified by that in 'chinese spring') in 9 or more genotypes from each of 15 populations of triticum dicoccoides in israel were determined. data also were obtained from 2 genotypes of the southernmost population (jaba). a single population from turkey was also investigated. there were 119 genotypes with translocations in the sample of 171 genotypes investigated (70%). the frequency of translocations in different popula ... | 1995 | 24169905 |
| wheat storage proteins: glutenin dna diversity in wild emmer wheat, triticum dicoccoids, in israel and turkey. 3. environmental correlates and allozymic associations. | the diversity of high-molecular-weight (hmw) and low-molecular-weight (lmw) glutenin subunits in the tetraploid wild progenitor of wheat, triticum dicoccoides, was studied at the dna level by the polymerase chain reaction (pcr). the dna diversity of hmw and lmw glutenins was shown to be correlated to environmental physical and biotic factors (climate, soil and pathogen resistance) and to allozyme variation. we conclude that glutenin dna diversity is nonrandomly distributed and could be more opti ... | 1995 | 24169830 |
| wheat storage proteins: glutenin diversity in wild emmer, triticum dicoccoides, in israel and turkey. 2. dna diversity detected by pcr. | germplasm of triticum dicoccoides collected from different environments in israel was evaluated by using the pcr as a molecular marker. two pairs of primers were used in the pcr in amplifying the dna tracts coding the high-and low-molecular-weight glutenin subunits. analyses reveal great variability within and between populations indicating the high values of this germplasm for future breeding programs to improve the protein quality in wheat. | 1995 | 24169829 |
| wheat phylogeny determined by rflp analysis of nuclear dna. 2. wild tetraploid wheats. | intra- and inter-specific variations in the nuclear dna of triticum dicoccoides körn. (2n = 28, genome constitution aabb) and t. araraticum jakubz. (2n = 28, aagg), wild species, respectively, of the emmer and timopheevi group, were studied by restriction fragment length polymorphism (rflp) analysis. total dnas of 32 t. dicoccoides and 24 t. araraticum accessions, collected from throughout the distribution areas of these species, were treated with two 6-bp cutters and hybridized with 30 nuclear ... | 1995 | 24173794 |
| c-banding analysis on wild emmer (triticum dicoccoides körn) strains with and without spontaneous reciprocal translocations. | c-banding polymorphism was analyzed in eight strains of wild emmer, triticum dicoccoides körn, which included six translocation homozygotes reported previously. polymorphisms were detected in all of the strains examined, and the breakpoints of five spontaneous translocations were successfully identified by c-bands. of the eight breakpoints that could be precisely identified, one was located in the centromeric region while the remaining seven were located in proximal to distal euchromatic regions ... | 1996 | 24166165 |
| high-density molecular map of chromosome region harboring stripe-rust resistance genes yrh52 and yr15 derived from wild emmer wheat, triticum dicoccoides. | two stripe-rust resistance genes, yrh52 and yr15, derived from the israeli wild emmer wheat, triticum dicoccoides, have been located on chromosome 1b. the main objectives of the present study were to increase marker density in the vicinity of yrh52 gene by means of aflp, rapd and microsatellite markers, to improve the map of another t dicoccoides-derived stripe-rust resistance gene yr15 using microsatellite markers, and to preliminarily discriminate these two genes. additional 26 marker loci com ... | 2000 | 11430483 |
| meiotic metaphase i pairing behavior of a 5bl recombinant isochromosome in wheat. | a recombinant isochromosome i5blrec of wheat was developed with one arm and the proximal 36% of the other arm of chinese spring (cs) origin and the distal 64% of the recombined arm of triticum turgidum subsp. dicoccoides origin. the i5blrec provides an unusual opportunity to analyze the role of the centromere or arm heterozygosity in chromosome prealignment and synapsis during meiosis. in monosomic condition, the i5blrec formed a ring univalent in 86.8% of the pollen mother cells (pmcs) at meiot ... | 2000 | 11196130 |
| coevolution of a and b genomes in allotetraploid triticum dicoccoides. | data is presented on the coevolution of a and b genomes in allotetraploid wheat triticum dicoccoides (2n = 4x = 28, genome aabb) obtained by genomic in situ hybridization (gish). probing chromosomes of t. dicoccoides with dna from the proposed a/b diploid genome ancestors shows evidence of enriching a-genome with repetitive sequences of b-genome type. thus, ancestral s-genome sequences have spread throughout the ab polyploid genome to a greater extent than have ancestral a-genome sequences. the ... | 2000 | 11195333 |
| molecular genetic maps in wild emmer wheat, triticum dicoccoides: genome-wide coverage, massive negative interference, and putative quasi-linkage. | the main objectives of the study reported here were to construct a molecular map of wild emmer wheat, triticum dicoccoides, to characterize the marker-related anatomy of the genome, and to evaluate segregation and recombination patterns upon crossing t. dicoccoides with its domesticated descendant triticum durum (cultivar langdon). the total map length exceeded 3000 cm and possibly covered the entire tetraploid genome (aabb). clusters of molecular markers were observed on most of the 14 chromoso ... | 2000 | 11042150 |
| microsatellite diversity correlated with ecological-edaphic and genetic factors in three microsites of wild emmer wheat in north israel. | this study was conducted to test the effects of internal (genetic) and external factors on allelic diversity at 27 dinucleotide microsatellite (simple sequence repeat [ssr]) loci in three israeli natural populations of triticum dicoccoides from ammiad, tabigha, and yehudiyya, north of the sea of galilee. the results demonstrated that ssr diversity is correlated with the interaction of ecological and genetic factors. genetic factors, including genome (a vs. b), chromosome, motif, and locus, affec ... | 2000 | 10833191 |
| comparative analysis of indices in the study of virulence diversity between and within populations of puccinia recondita f. sp. tritici in israel. | abstract isolates of puccinia recondita f. sp. tritici (n = 260) obtained from bread, durum, and wild emmer wheat leaf collections throughout israel during 1993 to 1997 were analyzed for virulence on a set of wheat differentials. the overall frequency of virulence increased on differentials possessing resistance genes lr1, lr2a, lr3, and lr26 and decreased on lr17, lr21, and lr30. genes lr9 and lr24 were resistant, while lr18 was susceptible (98% in 1996) to all tested leaf rust isolates and lr1 ... | 2000 | 18944539 |
| [molecular tagging of a major powdery mildew resistance gene mlg in wheat derived from wild emmer by using microsatellite marker]. | powdery mildew caused by erysiphe graminis f. sp. tritici is one of the most important wheat diseases in many regions of the world. breeding for resistant cultivars has been proved to be an effective and environmentally safe method to control diseases in wheat production. it is necessary to search for more resistance genes for the diversification of resistance genes in wheat breeding. an isreali wild emmer wheat (triticum dicoccoides) accession "g-305-m" was found resistant to the prevailing e. ... | 2001 | 11725638 |
| microsatellite polymorphism in natural populations of wild emmer wheat, triticum dicoccoides, in israel. | diversity in 20 microsatellite loci of wild emmer wheat, triticum dicoccoides, was examined in 15 populations (135 genotypes) representing a wide range of ecological conditions of soil, temperature, and water availability, in israel and turkey. an extensive amount of diversity at microsatellite loci was observed despite the predominantly selfing nature of this plant species. the 20 gatersleben wheat microsatellites (gwm), representing 13 chromosomes of genomes a and b of wheat, revealed a total ... | 2002 | 12579424 |
| climatic effects on microsatellite diversity in wild emmer wheat (triticum dicoccoides) at the yehudiyya microsite, israel. | microsatellite (ssr) diversity at 28 loci comprising seven types of tandem dinucleotide repeated motifs was analyzed in 105 individual plants of wild emmer wheat, triticum dicoccoides, from a microsite in yehudiyya, northeast of the sea of galilee, israel. the study area was less than 1000 m(2) and involved 12 paired plots distributed in a mosaic pattern. each experiment involved very close (a few meters apart), but sharply divergent, microclimatic niches in the open park forest of tabor oak: (1 ... | 2002 | 12136415 |
| repetitive dnas of wild emmer wheat (triticum dicoccoides) and their relation to s-genome species: molecular cytogenetic analysis. | we have analyzed the chromosomal gish molecular banding patterns of three populations of the wild allopolyploid wheat triticum dicoccoides in an attempt to unravel the evolutionary relationships between highly repetitive dna fractions of t. dicoccoides and proposed diploid progenitors of the b genome. aegilops speltoides showed almost complete affinity of its repetitive dna to c-heterochromatin of t. dicoccoides, whereas other s-genome species demonstrated relatedness only to distal heterochroma ... | 2002 | 11962636 |
| genetic effects on microsatellite diversity in wild emmer wheat (triticum dicoccoides) at the yehudiyya microsite, israel. | this study investigated allele size constraints and clustering, and genetic effects on microsatellite (simple sequence repeat, ssr) diversity at 28 loci comprising seven types of tandem repeated dinucleotide motifs in a natural population of wild emmer wheat, triticum dicoccoides, from a shade vs sun microsite in yehudiyya, northeast of the sea of galilee, israel. it was found that allele distribution at ssr loci is clustered and constrained with lower or higher boundary. this may imply that ssr ... | 2003 | 12634821 |
| domestication quantitative trait loci in triticum dicoccoides, the progenitor of wheat. | wild emmer wheat, triticum dicoccoides, is the progenitor of modern tetraploid and hexaploid cultivated wheats. our objective was to map domestication-related quantitative trait loci (qtl) in t. dicoccoides. the studied traits include brittle rachis, heading date, plant height, grain size, yield, and yield components. our mapping population was derived from a cross between t. dicoccoides and triticum durum. approximately 70 domestication qtl effects were detected, nonrandomly distributed among a ... | 2003 | 12604784 |
| precise mapping of a locus affecting grain protein content in durum wheat. | grain protein content (gpc) is an important factor in pasta and breadmaking quality, and in human nutrition. it is also an important trait for wheat growers because premium prices are frequently paid for wheat with high gpc. a promising source for alleles to increase gpc was detected on chromosome 6b of triticum turgidum var. dicoccoides accession fa-15-3 (dic). two previous quantitative trait locus (qtl) studies found that the positive effect of dic-6b was associated to a single locus located b ... | 2003 | 12923624 |
| chromosomal location of a triticum dicoccoides-derived powdery mildew resistance gene in common wheat by using microsatellite markers. | the powdery mildew resistance has been transferred from an israeli wild emmer (triticum dicoccoides) accession "g-305-m" into common wheat by crossing and backcrossing (g-305-m/781//jing 411*3). genetic analysis showed that the resistance was controlled by a single dominant gene at the seedling stage. among the 102 pairs of ssr primers tested, four polymorphic microsatellite markers (xpsp3029, xpsp3071, xpsp3152 and xgwm570) from the long arm of chromosome 6a were mapped in a bc(2)f(3) populatio ... | 2003 | 12582861 |
| genetic and physical mapping of a gene conditioning sensitivity in wheat to a partially purified host-selective toxin produced by stagonospora nodorum. | abstract a toxin, designated sntox1, was partially purified from culture filtrates of isolate sn2000 of stagonospora nodorum, the causal agent of wheat leaf and glume blotch. the toxin showed selective action on several different wheat genotypes, indicating that it is a host-selective toxin (hst). the toxic activity was reduced when incubated at 50 degrees c and activity was eliminated when treated with proteinase k, suggesting that the hst is a protein. the synthetic hexaploid wheat w-7984 and ... | 2004 | 18943793 |
| quantitative trait loci analysis and mapping of seedling resistance to stagonospora nodorum leaf blotch in wheat. | abstract stagonospora nodorum leaf blotch is an economically important foliar disease in the major wheat-growing areas of the world. in related work, we identified a host-selective toxin (hst) produced by the s. nodorum isolate sn2000 and determined the chromosomal location of the host gene (snn1) conditioning sensitivity to the toxin using the international triticeae mapping initiative mapping population and cytogenetic stocks. in this study, we used the same plant materials to identify quantit ... | 2004 | 18943794 |
| chromosomal location of genes for novel glutenin subunits and gliadins in wild emmer wheat (triticum turgidum l. var. dicoccoides). | the glutenin and gliadin proteins of wild emmer wheat, triticum turgidum l. var. dicoccoides, have potential for improvement of durum wheat ( t. turgidum l. var. durum) quality. the objective of this study was to determine the chromosomes controlling the high molecular weight (hmw) glutenin subunits and gliadin proteins present in three t. turgidum var. dicoccoides accessions (israel-a, pi-481521, and pi-478742), which were used as chromosome donors in langdon durum- t. turgidum var. dicoccoides ... | 2004 | 14727030 |
| a genetic linkage map of the durum x triticum dicoccoides backcross population based on ssrs and aflp markers, and qtl analysis for milling traits. | durum wheat ( triticum turgidum l. var durum) is mainly produced and consumed in the mediterranean region; it is used to produce several specific end-products; such as local pasta, couscous and burghul. to study the genetics of grain-milling quality traits, chromosomal locations, and interaction with the environment, a genetic linkage map of durum was constructed and the quantitative trait loci qtls for the milling-related traits, test weight (tw) and thousand-kernel weight (tkw), were identifie ... | 2004 | 14676946 |
| application of fluorescence-based resistance gene analog analysis for genotyping plant genetic resources. | conventional approaches for detecting disease resistance gene analogs (rgas) in plants are based on agarose gels or on polyacrylamide gel electrophoresis (page) in combination with silver staining or radioactive labeling. a modified method for rga analysis has been developed by using fluorescence-labeled primers for pcr amplifications. the amplified fragments are detected by denaturing page using an automated laser fluorescence dna sequencer and analyzed by fragment analysis software. this techn ... | 2005 | 15703869 |
| a reconsideration of the domestication geography of tetraploid wheats. | the domestication of tetraploid wheats started from their wild progenitor triticum dicoccoides. in this paper, the geographical distribution of this progenitor is revised to include more sampling locations. the paper is based on a collection of wild and domesticated lines (226 accessions in total) analyzed by aflp at 169 polymorphic loci. the collection includes the 69 wild lines considered by mori et al. (2003) in their study on chloroplast dna haplotypes of t. dicoccoides. the goal of the expe ... | 2005 | 15714326 |
| isolation of low-molecular-weight glutenin subunit genes from wild emmer wheat (triticum dicoccoides). | three low-molecular-weight glutenin subunit (lmw-gs) genes, designated lmw-td1, lmw-td2 and lmw-td3, were isolated from wild emmer wheat (triticum dicoccoides), which is the tetraploid progenitor of common wheat (t. aestivum). the complete nucleotide sequence lengths of lmw-td1, lmw-td2 and lmw-td3 are 858, 900 and 1062 bp, respectively. lmw-td1 and lmw-td3 can encode proteins with 284 and 352 amino acid residues, respectively, whereas lmw-td2 is a putative pseudogene due to the presence of 3 in ... | 2005 | 16278506 |
| two point mutations identified in emmer wheat generate null wx-a1 alleles. | in this report, the wx-a1 mutations carried by a triticum dicoccoides line from israel and a triticum dicoccum line from yugoslavia are characterized. a single nucleotide insertion in the t. dicoccoides null allele and a single nucleotide deletion in the t. dicoccum null allele each cause frameshift mutations that induce premature termination codons more than 55 nucleotides upstream of the last exon-exon junction. in both mutants, wx-a1 transcripts were detectable in 10 day post-anthesis endospe ... | 2005 | 15592661 |
| molecular characterization of of alpha-gliadin genes from wild emmer wheat (triticum dicoccoides). | according to the two distal and conserved regions of known alpha-gliadin genes, gene-specific primers for alpha-gliadin were designed, and the coding regions of four gliadin genes (i.e. glitd-1, glitd-2, glitd-3 and glitd-4) with the length of about 800 bp were isolated from the genomic dna of wild emmer wheat (triticum dicoccoides). no introns were observed. sequence comparison indicated that these genes should be classified as alpha-gliadins. glitd-3 (genbank accession no.dq140351) and glitd-4 ... | 2006 | 17381042 |
| molecular characterization of a hmw glutenin subunit allele providing evidence for silencing of x-type gene on glu-b1. | understanding the molecular structure of high-molecular-weight glutenin subunit (hmw-gs) may provide useful evidence for the study on the improvement of quality of cultivated wheat and the evolution of glu-1 alleles. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) shows that the subunits encoded by glu-b1 were null, named 1bxm, in a triticum turgidum var. dicoccoides line pi94640. primers based on the conserved regions in wheat hmw-gs gene promoter and coding sequences were ... | 2006 | 17046593 |
| spatio-temporal genetic variation in populations of wild emmer wheat, triticum turgidum ssp. dicoccoides, as revealed by aflp analysis. | genetic structure of natural populations of wild crop relatives has been the subject of many studies. yet, most of them focused on the assessment of spatial genetic diversity, while information on long-term variation, affected by yearly changes, has been considered only in few cases. the present study aimed therefore, to estimate the spatio-temporal genetic variation in populations of wild emmer wheat, the progenitor of domesticated wheat, and to assess the contribution of spatial versus tempora ... | 2007 | 17447050 |
| independent wheat b and g genome origins in outcrossing aegilops progenitor haplotypes. | the origin of modern wheats involved alloploidization among related genomes. to determine if aegilops speltoides was the donor of the b and g genomes in aabb and aagg tetraploids, we used a 3-tiered approach. using 70 amplified fragment length polymorphism (aflp) loci, we sampled molecular diversity among 480 wheat lines from their natural habitats encompassing all s genome aegilops, the putative progenitors of wheat b and g genomes. fifty-nine aegilops representatives for s genome diversity wer ... | 2007 | 17053048 |
| molecular evolution of dimeric alpha-amylase inhibitor genes in wild emmer wheat and its ecological association. | alpha-amylase inhibitors are attractive candidates for the control of seed weevils, as these insects are highly dependent on starch as an energy source. in this study, we aimed to reveal the structure and diversity of dimeric alpha-amylase inhibitor genes in wild emmer wheat from israel and to elucidate the relationship between the emmer wheat genes and ecological factors using single nucleotide polymorphism (snp) markers. another objective of this study was to find out whether there were any co ... | 2008 | 18366725 |
| molecular mapping of the novel powdery mildew resistance gene pm36 introgressed from triticum turgidum var. dicoccoides in durum wheat. | powdery mildew, caused by blumeria graminis f.sp. tritici, is one of the most important wheat diseases in many regions of the world. triticum turgidum var. dicoccoides (2n=4x=aabb), the progenitor of cultivated wheats, shows particular promises as a donor of useful genetic variation for several traits, including disease resistances. the wild emmer accession mg29896, resistant to powdery mildew, was backcrossed to the susceptible durum wheat cultivar latino, and a set of backcross inbred lines (b ... | 2008 | 18392800 |
| wheat leaf rust caused by puccinia triticina. | leaf rust, caused by puccinia triticina, is the most common rust disease of wheat. the fungus is an obligate parasite capable of producing infectious urediniospores as long as infected leaf tissue remains alive. urediniospores can be wind-disseminated and infect host plants hundreds of kilometres from their source plant, which can result in wheat leaf rust epidemics on a continental scale. this review summarizes current knowledge of the p. triticina/wheat interaction with emphasis on the infecti ... | 2008 | 19018988 |
| molecular cloning and characterization of four novel lmw glutenin subunit genes from aegilops longissima, triticum dicoccoides and t. zhukovskyi. | this paper reports cloning and characterisation of four novel low-molecular-weight glutenin subunit (lmw-gs) genes (designated as tzlmw-m2, tzlmw-m1, tdlmw-m1 and allmw-m2) from the genomic dna of triticum dicoccoides, t. zhukovskyi and aegilops longissima. the coding regions of tzlmw-m2, tzlmw-m1, tdlmw-m1 and allmw-m2 were 1056 bp, 903 bp, 1056 bp and 1050 bp in length, encoding 350, 300, 350 and 348 amino acid residues, respectively. the deduced amino acid sequences showed that the four novel ... | 2008 | 18503711 |
| genetic structure of wild emmer wheat populations as reflected by transcribed versus anonymous ssr markers. | simple sequence repeat (ssr) markers have become a major tool in population genetic analyses. the anonymous genomic ssrs (gssrs) have been recently supplemented with expressed sequence tag (est) derived ssrs (essrs), which represent the transcribed regions of the genome. in the present study, we used 8 populations of wild emmer wheat (triticum turgidum subsp. dicoccoides) to compare the usefulness of the two types of ssr markers in assessing allelic diversity and population structure. gssrs reve ... | 2008 | 18356954 |
| allelic diversity associated with aridity gradient in wild emmer wheat populations. | the association between allelic diversity and ecogeographical variables was studied in natural populations of wild emmer wheat [triticum turgidum ssp. dicoccoides (körn.) thell.], the tetraploid progenitor of cultivated wheat. patterns of allelic diversity in 54 microsatellite loci were analyzed in a collection of 145 wild emmer wheat accessions representing 25 populations that were sampled across naturally occurring aridity gradient in israel and surrounding regions. the obtained results reveal ... | 2008 | 17908203 |
| divergent diversity patterns of nbs and lrr domains of resistance gene analogs in wild emmer wheat populations. | disease resistance (r) genes are intriguing in their evolution and diversity patterns because of their constant interactions with evolving pathogens. in this study, we demonstrate the use of resistance gene analog (rga) markers to estimate genetic diversity among 13 populations (118 genotypes) of triticum dicoccoides collected along a natural aridity gradient in israel. the diversity patterns of 204 markers derived from two r-gene domains, nucleotide binding site (nbs) and leucine-rich repeat (l ... | 2009 | 19483774 |
| quantitative trait loci conferring grain mineral nutrient concentrations in durum wheat x wild emmer wheat ril population. | mineral nutrient malnutrition, and particularly deficiency in zinc and iron, afflicts over 3 billion people worldwide. wild emmer wheat, triticum turgidum ssp. dicoccoides, genepool harbors a rich allelic repertoire for mineral nutrients in the grain. the genetic and physiological basis of grain protein, micronutrients (zinc, iron, copper and manganese) and macronutrients (calcium, magnesium, potassium, phosphorus and sulfur) concentration was studied in tetraploid wheat population of 152 recomb ... | 2009 | 19407982 |
| [an improved method of genomic in situ hybridization (gish) for distinguishing closely related genomes of tetraploid and hexaploid wheat species]. | an improved modification of genomic in situ hybridization (gish) was proposed. it allows clear and reproducible discrimination between closely related genomes of both tetraploid and hexaploid wheat species due to preannealing of labeled dna probes and prehybridization of chromosomal samples with blocking dna. the method was applied to analyze intergenomic translocations 6a:6b and 1a:6b identified in the ig46147 and ig116188 samples of tetraploid wheat triticum dicoccoides by c-banding. the struc ... | 2009 | 19405447 |
| est-ssr diversity correlated with ecological and genetic factors of wild emmer wheat in israel. | the differentiation of genetic diversity was estimated among 15 wild emmer wheat (triticum dicoccoides) populations of the macrogeographic scale in israel by 25 est-ssr markers. a total of 92 est-ssr alleles were detected, and the number of alleles ranged from 1 to 7 with an average of 3.68 per locus. allele numbers and the polymorphic information content (pic)value of est-ssr loci on the b genome were higher than those on the a genome. the genetic similarity coefficient (gs)varied from 0.189 to ... | 2009 | 19360986 |
| quantitative trait locus mapping of increased fusarium head blight susceptibility associated with a wild emmer wheat chromosome. | chromosome 2a of wild emmer wheat (triticum turgidum var. dicoccoides) genotype israel a increases fusarium head blight (fhb) severity when present in durum wheat (t. turgidum var. durum) cv. langdon (ldn). the goal of this study was to identify regions of israel a chromosome 2a associated with this difference in resistance. a recombinant inbred chromosome line population (ricl) from a cross between ldn and the ldn-israel a chromosome 2a substitution line [ldn(dic-2a)] was employed for analysis. ... | 2009 | 19271987 |
| genomic dissection of drought resistance in durum wheat x wild emmer wheat recombinant inbreed line population. | drought is the major factor limiting wheat productivity worldwide. the gene pool of wild emmer wheat, triticum turgidum ssp. dicoccoides, harbours a rich allelic repertoire for morpho-physiological traits conferring drought resistance. the genetic and physiological bases of drought responses were studied here in a tetraploid wheat population of 152 recombinant inbreed lines (rils), derived from a cross between durum wheat (cv. langdon) and wild emmer (acc# g18-16), under contrasting water availa ... | 2009 | 19220786 |
| sequencing over 13 000 expressed sequence tags from six subtractive cdna libraries of wild and modern wheats following slow drought stress. | a deeper understanding of the drought response and genetic improvement of the cultivated crops for better tolerance requires attention because of the complexity of the drought response syndrome and the loss of genetic diversity during domestication. we initially screened about 200 wild emmer wheat genotypes and then focused on 26 of these lines, which led to the selection of two genotypes with contrasting responses to water deficiency. six subtractive cdna libraries were constructed, and over 13 ... | 2009 | 19054353 |
| [intraspecific divergence in wheats of the emmer group using in situ hybridization with the spelt1 family of tandem repeats]. | fluorescence in situ hybridization (fish) was used to study the distribution of spelt1 repetitive dna sequences on chromosomes of 37 accessions representing eight polyploidy wheat species of the emmer evolutionary lineage: triticum dicoccoides körn, t. dicoccum (schrank) schuebel, t. durum desf., t. polonicum l., t. carthlicum nevski, t. aethiopicum jabbz., t. aestivum l., and t. spelta l. substantial polymorphism in the number, distribution, and the sizes of the spelt1 loci was revealed. on the ... | 2009 | 20058802 |
| identification and genetic mapping of pm42, a new recessive wheat powdery mildew resistance gene derived from wild emmer (triticum turgidum var. dicoccoides). | powdery mildew, caused by blumeria graminis f. sp. tritici, is one of the most important wheat diseases worldwide in areas with cool or maritime climates. wild emmer (triticum turgidum var. dicoccoides) is an important potential donor of disease resistances and other traits for common wheat improvement. a powdery mildew resistance gene was transferred from wild emmer accession g-303-1m to susceptible common wheat by crossing and backcrossing, resulting in inbred line p63 (yanda1817/g-303-1 m//3* ... | 2009 | 19407985 |
| molecular identification of a new powdery mildew resistance gene pm41 on chromosome 3bl derived from wild emmer (triticum turgidum var. dicoccoides). | powdery mildew caused by blumeria graminis f. sp. tritici is an important wheat disease in china and other parts of the world. wild emmer (triticum turgidum var. dicoccoides) is the immediate progenitor of cultivated tetraploid and hexaploid wheats and thus an important resource for wheat improvement. wild emmer accession iw2 collected from mount hermon, israel, is highly resistant to powdery mildew at the seedling and adult plant stages. genetic analysis using an f(2) segregating population and ... | 2009 | 19471905 |
| independent evolution of functional pm3 resistance genes in wild tetraploid wheat and domesticated bread wheat. | the pm3 alleles of cultivated bread wheat confer gene for gene resistance to the powdery mildew fungus. they represent a particular case of plant disease resistance gene evolution, because of their recent origin and possible evolution after the formation of hexaploid wheat. the pm3 locus is conserved in tetraploid wheat, thereby allowing the comparative evolutionary study of the same resistance locus in a domesticated species and in one of its wild ancestors. we have identified 61 pm3 allelic se ... | 2009 | 18980638 |
| transcriptome pathways unique to dehydration tolerant relatives of modern wheat. | among abiotic stressors, drought is a major factor responsible for dramatic yield loss in agriculture. in order to reveal differences in global expression profiles of drought tolerant and sensitive wild emmer wheat genotypes, a previously deployed shock-like dehydration process was utilized to compare transcriptomes at two time points in root and leaf tissues using the affymetrix genechip(r) wheat genome array hybridization. the comparison of transcriptomes reveal several unique genes or express ... | 2009 | 19330365 |
| ecological genomics of natural plant populations: the israeli perspective. | the genomic era revolutionized evolutionary population biology. the ecological genomics of the wild progenitors of wheat and barley reviewed here was central in the research program of the institute of evolution, university of haifa, since 1975 ( http://evolution.haifa.ac.il ). we explored the following questions: (1) how much of the genomic and phenomic diversity of wild progenitors of cultivars (wild emmer wheat, triticum dicoccoides, the progenitor of most wheat, plus wild relatives of the ae ... | 2009 | 19347652 |
| pathogen race determines the type of resistance response in the stripe rust-triticum dicoccoides pathosystem. | wild relatives of crop plants may serve as a promising source for screening for new disease resistance genes that can be utilized in breeding programs. triticum dicoccoides, the wild progenitor of most cultivated wheats, was shown to harbor many resistance genes against the major diseases attacking cultivated wheat. stripe rust is a devastating fungal disease that attacks wheat in many regions of the world. new races of puccinia striiformis westend. f. sp. tritici, the causative agent of stripe ... | 2010 | 20163557 |
| identification and mapping of pmg16, a powdery mildew resistance gene derived from wild emmer wheat. | the gene-pool of wild emmer wheat, triticum turgidum ssp. dicoccoides, harbors a rich allelic repertoire for disease resistance. in the current study, we made use of tetraploid wheat mapping populations derived from a cross between durum wheat (cv. langdon) and wild emmer (accession g18-16) to identify and map a new powdery mildew resistance gene derived from wild emmer wheat. initially, the two parental lines were screened with a collection of 42 isolates of blumeria graminis f. sp. tritici (bg ... | 2010 | 20407741 |
| genetic and comparative genomics mapping reveals that a powdery mildew resistance gene ml3d232 originating from wild emmer co-segregates with an nbs-lrr analog in common wheat (triticum aestivum l.). | powdery mildew caused by blumeria graminis f. sp. tritici is one of the most important wheat diseases worldwide and breeding for resistance using diversified disease resistance genes is the most promising approach to prevent outbreaks of powdery mildew. a powdery mildew resistance gene, originating from wild emmer wheat (triticum turgidum var. dicoccoides) accessions collected from israel, has been transferred into the hexaploid wheat line 3d232 through crossing and backcrossing. inoculation res ... | 2010 | 20686747 |
| the 3adon population of fusarium graminearum found in north dakota is more aggressive and produces a higher level of don than the prevalent 15adon population in spring wheat. | fusarium head blight (fhb) is primarily caused by fusarium graminearum in north america. isolates of f. graminearum can be identified as one of three chemotypes: 3-acetyl-deoxynivalenol (3adon), 15-acetyl-deoxynivalenol (15adon), and nivalenol (niv). in this study, we characterized f. graminearum isolates collected in 1980 to 2000 (old collection) and in 2008 (new collection) from north dakota and found a 15-fold increase of 3adon isolates in the new collection. evaluation of randomly selected 3 ... | 2010 | 20839936 |
| mapping genes lr53 and yr35 on the short arm of chromosome 6b of common wheat with microsatellite markers and studies of their association with lr36. | the rust resistance genes lr53 and yr35, transferred to common wheat from triticum dicoccoides, were reported previously to be completely linked on chromosome 6b. four f (3) families were produced from a cross between a line carrying lr53 and yr35 (98m71) and the leaf rust and stripe rust susceptible genotype avocet "s" and were rust tested using puccinina triticina pathotype 53-1,(6),(7),10,11 and puccinia striiformis f. sp. tritici pathotype 110 e143 a+. the homozygous resistant lines produced ... | 2010 | 20924745 |
| drought and salt tolerances in wild relatives for wheat and barley improvement. | drought and salinity are the major abiotic stresses that dramatically threaten the food supply in the world. tribe triticeae, including wheat and barley, possesses tremendous potential for drought and salt tolerance that has been extensively and practically identified, tested, and transferred to wheat cultivars with proven expression of tolerance in experimental trials. triticum dicoccoides and hordeum spontaneum, the progenitors of cultivated wheat and barley, have adapted to a broad range of e ... | 2010 | 20040064 |
| the impact of single nucleotide polymorphism in monomeric alpha-amylase inhibitor genes from wild emmer wheat, primarily from israel and golan. | various enzyme inhibitors act on key insect gut digestive hydrolases, including alpha-amylases and proteinases. alpha-amylase inhibitors have been widely investigated for their possible use in strengthening a plant's defense against insects that are highly dependent on starch as an energy source. we attempted to unravel the diversity of monomeric alpha-amylase inhibitor genes of israeli and golan heights' wild emmer wheat with different ecological factors (e.g., geography, water, and temperature ... | 2010 | 20534122 |
| multilevel regulation and signalling processes associated with adaptation to terminal drought in wild emmer wheat. | low water availability is the major environmental factor limiting crop productivity. transcriptome analysis was used to study terminal drought response in wild emmer wheat, triticum dicoccoides, genotypes contrasting in their productivity and yield stability under drought stress. a total of 5,892 differentially regulated transcripts were identified between drought and well-watered control and/or between drought resistant (r) and drought susceptible (s) genotypes. functional enrichment analyses r ... | 2010 | 20333536 |
| genetic diversity for grain nutrients in wild emmer wheat: potential for wheat improvement. | micronutrient malnutrition, particularly zinc and iron deficiency, afflicts over three billion people worldwide due to low dietary intake. in the current study, wild emmer wheat (triticum turgidum ssp. dicoccoides), the progenitor of domesticated wheat, was tested for (1) genetic diversity in grain nutrient concentrations, (2) associations among grain nutrients and their relationships with plant productivity, and (3) the association of grain nutrients with the eco-geographical origin of wild emm ... | 2010 | 20202969 |
| dehydration stress-responsive mirna in brachypodium distachyon: evident by genome-wide screening of micrornas expression. | there is a lack of knowledge on the tissue-specific expression of mirnas in response to dehydration stress in brachypodium (brachypodium distachyon (l.) beauv), a model for temperate grass species. in this study, mirna expression patterns of drought-tolerant brachypodium were investigated using the mirna microarray platform. a total of 205 mirnas in control and 438 mirnas in both drought-treated leaf and root tissues were expressed. seven of the detected brachypodium mirnas were dehydration stre ... | 2011 | 22122669 |
| genomic, rna, and ecological divergences of the revolver transposon-like multi-gene family in triticeae. | revolver is a newly discovered multi-gene family of transposable elements in the triticeae genome. revolver encompasses 2929 to 3041 bp, has 20 bp of terminal inverted repeated sequences at both ends, and contains a transcriptionally active gene encoding a dna-binding-like protein. a putative tata box is located at base 221, with a cap site at base 261 and a possible polyadenylation signal aataaa at base 2918. revolver shows considerable quantitative variation in wheat and its relatives. | 2011 | 21943048 |
| genetic analysis of wheat domestication and evolution under domestication. | wheat is undoubtedly one of the world's major food sources since the dawn of near eastern agriculture and up to the present day. morphological, physiological, and genetic modifications involved in domestication and subsequent evolution under domestication were investigated in a tetraploid recombinant inbred line population, derived from a cross between durum wheat and its immediate progenitor wild emmer wheat. experimental data were used to test previous assumptions regarding a protracted domest ... | 2011 | 21778183 |
| alteration in expression of hormone-related genes in wild emmer wheat roots associated with drought adaptation mechanisms. | transcriptomic and metabolomic profiles were used to unravel drought adaptation mechanisms in wild emmer wheat (triticum turgidum ssp. dicoccoides), the progenitor of cultivated wheat, by comparing the response to drought stress in roots of genotypes contrasting in drought tolerance. the differences between the drought resistant (r) and drought susceptible (s) genotypes were characterized mainly by shifts in expression of hormone-related genes (e.g., gibberellins, abscisic acid (aba) and auxin), ... | 2011 | 21656015 |
| mirna expression patterns of triticum dicoccoides in response to shock drought stress. | drought is a major environmental stress factor that affects plant growth and development worldwide. wild emmer wheat (triticum turgidum ssp. dicoccoides), the ancestor of domesticated durum wheat (triticum turgidum ssp. durum), has great potential for improving the understanding of the wheat drought response. micrornas (mirnas) are a recently discovered class of gene expression regulators that have also been linked to several plant stress responses; however, this relationship is just beginning t ... | 2011 | 21069383 |
| rapid linkage disequilibrium decay in the lr10 gene in wild emmer wheat (triticum dicoccoides) populations. | recombination is a key evolutionary factor enhancing diversity. however, the effect of recombination on diversity in inbreeding species is expected to be low. to estimate this effect, recombination and diversity patterns of lr10 gene were studied in natural populations of the inbreeder species, wild emmer wheat (triticum dicoccoides). wild emmer wheat is the progenitor of most cultivated wheats and it harbors rich genetic resources for disease resistance. lr10 is a leaf rust resistance gene enco ... | 2011 | 20859611 |
| tmpit1 from wild emmer wheat: first characterisation of a stress-inducible integral membrane protein. | in this study a gene for a drought stress-inducible putative membrane protein was cloned and characterised from root tissue of wild emmer wheat. sequence analysis indicated that the protein is a member of the widespread but hitherto uncharacterised tmpit (transmembrane protein inducible by tnf-α) family, so it was labelled tdictmpit1. real-time rt-pcr showed that the tdictmpit1 gene is upregulated on drought stress in drought-tolerant wild emmer wheat, but not in a drought-sensitive accession or ... | 2011 | 21635942 |
| identification and characterization of a novel powdery mildew resistance gene pmg3m derived from wild emmer wheat, triticum dicoccoides. | powdery mildew, caused by blumeria graminis f. sp. tritici (bgt) is one of the most important wheat diseases worldwide. wild emmer wheat, triticum turgidum ssp. dicoccoides, the tetraploid ancestor (aabb) of domesticated bread and durum wheat, harbors many important alleles for resistance to various diseases, including powdery mildew. in the current study, two tetraploid wheat mapping populations, derived from a cross between durum wheat (cv. langdon) and wild emmer wheat (accession g-305-3m), w ... | 2011 | 22159825 |
| identification and comparative mapping of a powdery mildew resistance gene derived from wild emmer (triticum turgidum var. dicoccoides) on chromosome 2bs. | powdery mildew, caused by blumeria graminis f. sp. tritici, is an important foliar disease of wheat worldwide. wild emmer (triticum turgidum var. dicoccoides) is a valuable genetic resource for improving disease resistance in common wheat. a powdery mildew resistance gene conferring resistance to b. graminis f. sp. tritici isolate e09 at the seedling and adult stages was identified in wild emmer accession iw170 introduced from israel. an incomplete dominant gene, temporarily designated mliw170, ... | 2011 | 22170431 |
| ancient diversity of splicing motifs and protein surfaces in the wild emmer wheat (triticum dicoccoides) lr10 coiled coil (cc) and leucine-rich repeat (lrr) domains. | in this study, we explore the diversity and its distribution along the wheat leaf rust resistance protein lr10 three-dimensional structure. lr10 is a leaf rust resistance gene encoding a coiled coil-nucleotide-binding site-leucine-rich repeat (cc-nbs-lrr) class of protein. lr10 was cloned and sequenced from 58 accessions representing diverse habitats of wild emmer wheat in israel. nucleotide diversity was very high relative to other wild emmer wheat genes (π= 0.029). the cc domain was found to b ... | 2011 | 21952112 |
| the drought response displayed by a dre-binding protein from triticum dicoccoides. | drought is one of the major causes of dramatic yield loss in crop plants. knowledge of how to alleviate this loss is still limited due to the complexity of both the stress condition and plant responses. wild emmer wheat (triticum turgidum ssp. dicoccoides) is a potential source of important drought-resistance genes for its cultivated relatives. the gene for an emmer dre-binding protein, tdicdrf1, was cloned and shown to be drought-responsive with orthologs in other plants. this is the first repo ... | 2011 | 21296583 |
| evolution and dispersal of emmer wheat (triticum sp.) from novel haplotypes of ppd-1 (photoperiod response) genes and their surrounding dna sequences. | the sequence data from 5' utr, intronic, coding and 3' utr regions of ppd-a1 and ppd-b1 were investigated for a total of 158 accessions of emmer wheat landraces comprising 19 of wild emmer wheat (triticum dicoccoides), 45 of hulled emmer wheat (t. dicoccum) and 94 of free-threshing (ft) emmer wheat (t. durum etc.). we detected some novel types of deletions in the coding regions from 22 hulled emmer accessions and 20 ft emmer accessions. emmer wheat accessions with these deletions could produce p ... | 2012 | 22639190 |
| identification and molecular characterisation of hmw glutenin subunit 1by16* in wild emmer. | in this study, a novel y-type high molecular weight glutenin subunit (hmw-gs) in wild emmer wheat triticum turgidum l. var. dicoccoides (körn.) accession ku1952 was identified by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page), capillary electrophoresis (ce) and matrix-assisted laser desorption ionisation/time-of-flight/mass spectrometry (maldi-tof-ms). its electrophoretic mobility and molecular weight were similar to those of 1by16 and was designated as 1by16*. the complet ... | 2012 | 22644727 |
| plant genetic resources: prediction by isozyme markers and ecology. | linkage or association of genetic markers to quantitative traits of agronomic importance can substantially simplify the genetic analysis of complex quantitative traits. enzyme marker genes are ideal candidates for quantitative genetic analysis. we have recently applied this powerful methodology to the analysis of genetic resources of wild cereals in israel, primarily wild emmer wheat, triticum dicoccoides, and wild barley, hordeum spontaneum, the progenitors of cultivated wheats and barley, resp ... | 2012 | 3610595 |
| autophagy-related gene, tdatg8, in wild emmer wheat plays a role in drought and osmotic stress response. | an autophagy-related gene atg8 was cloned for the first time from wild emmer wheat, named as tdatg8, and its role on autophagy under abiotic stress conditions was investigated. examination of tdatg8 expression patterns indicated that atg8 expression was strongly upregulated under drought stress, especially in the roots when compared to leaves. lysotracker(®) red marker, utilized to observe autophagosomes, revealed that autophagy is constitutively active in triticum dicoccoides. moreover, autopha ... | 2012 | 22569921 |
| high-density mapping and marker development for the powdery mildew resistance gene pmas846 derived from wild emmer wheat (triticum turgidum var. dicoccoides). | powdery mildew, caused by blumeria graminis f. sp. tritici, is an important foliar disease of wheat worldwide. the dominant powdery mildew resistance gene pmas846 was transferred to the hexaploid wheat lines n9134 and n9738 from wild emmer wheat (triticum dicoccoides) in 1995, and it is still one of the most effective resistance genes in china. a high resolution genetic map for pmas846 locus was constructed using two f(2) populations and corresponding f(2:3) families developed from the crosses o ... | 2012 | 22350087 |
| [genetics determination of wheat resistance to puccinia graminis f. sp. tritici deriving from aegilops cylindrica, triticum erebuni and amphidiploid 4]. | the lines of winter soft wheat developed in the plant breeding and genetics institute contain new effective introgressive sr-genes. line 85/06 possess srac1 gene, lines 47/06, 54/06, 82/06, 85/06, 87/06, 238/06, and 367/06 possess srac1 and srac2 derived from aegilops cylindrica, line 352/06 - srte1 and srte2 from triticum erebuni, line 12/86-04 - srad1 and srad2 from amphidiploid 4 (triticum dicoccoides x triticum tauschii). | 2012 | 22420215 |
| drought tolerance in modern and wild wheat. | the genus triticum includes bread (triticum aestivum) and durum wheat (triticum durum) and constitutes a major source for human food consumption. drought is currently the leading threat on world's food supply, limiting crop yield, and is complicated since drought tolerance is a quantitative trait with a complex phenotype affected by the plant's developmental stage. drought tolerance is crucial to stabilize and increase food production since domestication has limited the genetic diversity of crop ... | 2013 | 23766697 |