Publications

comparative study of seven picornaviruses of man.	jamison, richard m. (baylor university college of medicine, houston, tex.), and heather d. mayor. comparative study of seven picornaviruses of man. j. bacteriol. 91:1971-1976. 1966.-the buoyant densities in cscl and potassium tartrate of seven acid-resistant picornaviruses of man were determined. the viruses studied were found to be a relatively homogeneous group. buoyant densities ranged from 1.32 to 1.35 g/cc in cscl and 1.21 to 1.23 g/cc in potassium tartrate. the buoyant density of each viru ...	1966	4287079
hand, foot and mouth disease associated with coxsackievirus group b.	this study from frankfurt, although similar to other reports with respect to clinical findings, presents further evidence of a varied etiology for hand, foot and mouth disease (hfmd). of the 6 patients with hfmd, 5 ahd virologic evidence of coxsackievirus b5 infection, and 1 had definite evidence of coxsackievirus b2 infection. our data suggest an etiologic association of coxsackievirus group b with hfmd.	1975	1179172
coxsackievirus aggregates in muscle cells of a polymyositis patient.	muscle biopsy specimens of a patient with polymyositis showed crystalline structures resembling picornavirus aggregates within muscle cells. the patient's serum reacted in an indirect immunofluorescence assay with autologous muscle cells. a strongly positive immunofluorescence staining was also noted when a section of muscle tissue was reacted with coxsackievirus a9 antiserum, and a weakly positive reaction was noted with coxsackievirus b2 antiserum. no staining was observed after treatment with ...	1978	352989
coxsackievirus b epidemic at a boys' camp.	an epidemic of coxsackievirus b2 infections occured at a boys summer camp. the resulting illness was characterized by malaise, headache, muscle pain, and high fever that persisted for four to six days. the boy in the index case arrived at the camp the first day of the season. the attack rate was 89% among campers and 47% among counselors. the spread of infections appeared to be on a person-to-person basis and in a disorderly fashion. unusual features of the epidemic included the high attack rate ...	1979	223433
computerized tomography in infantile encephalitis.	although computerized tomographic (ct) findings are well recognized in numerous neurologic diseases of children, ct scan abnormalities of encephalitis have only been associated with herpes simplex infection. we describe two infants, one with coxsackievirus b2 meningoencephalitis and one with clinical encephalitis, whose ct scans were similarly abnormal during the acute phase of their illness. in both infants, recovery of both neurologic and developmental deficits was accompanied by resolution of ...	1979	223434
procedure for recovery of enteroviruses from the japanese cockle tapes japonica.	the most likely shellfish to be harvested if sportfishing is reinstated in san francisco bay is the japanese cockle tapes japonica. the virus levels present in these shellfish are unknown and need to be evaluated before the shellfish beds are open. towards this end, a procedure for recovering and concentrating enteric viruses from these clams has been evaluated. effective elution of poliovirus from clam tissues was found to occur with ph 9.5 glycine-buffered saline rather than with the ph 7.5 fl ...	1981	6263186
serum substitute in epithelial cell culture media: nonfat dry milk filtrate.	a number of milk types and milk fractions were investigated as possible substitutes for serum in cell culture media. a filtrate of reconstituted nonfat dry milk showed promise. culture fluids containing 5% of the nonfat dry milk filtrate were used to propagate primary and continuous cell cultures, and the cell growth from these cultures was compared with that of cells grown in a serum-containing medium. the nonfat dry milk filtrate-supplemented medium supported the growth of all epithelial cells ...	1981	6269491
[infections of the central nervous system caused by enterovirus: 223 cases seen at a pediatric hospital between 1973 and 1981].	between 1973 and 1981, 223 patients were seen at hôpital sainte-justine in montreal for enteroviral infection of the nervous system. in 161 the cause was documented by isolation of an enterovirus from the cerebrospinal fluid (csf). the viruses most frequently isolated were echovirus 11 (36 isolates), echovirus 30 (24), coxsackievirus b2 (23), coxsackievirus b3 (19), echovirus 6 (18), coxsackievirus b5 (16), coxsackievirus a9 (15), echovirus 9 (13), echovirus 7 (12) and coxsackievirus b1 (11). as ...	1982	6291736
coxsackievirus b2 infection in a neonate with incontinentia pigmenti.	because of the concern for herpes simplex virus infection in the neonate, the presence of neonatal vesiculobullous lesions is a critical finding. however, there are other etiologies for these lesions. a case of a neonate with a vesicular rash and meningoencephalitis which was initially thought due to herpes is presented. the infant was ultimately determined to have incontinentia pigmenti and a concomitant coxsackievirus b2 infection.	1986	3012453
end-stage dilated cardiomyopathy. persistence of enterovirus rna in myocardium at cardiac transplantation and lack of immune response.	tissue from the explanted hearts of 21 patients with idiopathic dilated cardiomyopathy or 19 patients with other specific heart muscle diseases were investigated for presence of enterovirus-specific rna with an enterovirus group-specific cdna probe. this was complementary to coxsackievirus b2 rna sequences between nucleotide numbers 6,550 and 7,400, which are highly conserved between enteroviruses. hearts from six patients with dilated cardiomyopathy and one patient with ischemic heart disease w ...	1989	2553297
evidence for a group-specific enteroviral antigen(s) recognized by human t cells.	human peripheral blood mononuclear cells from 15 normal, healthy adult volunteers proliferated in vitro against a panel of enteroviral antigens, including coxsackievirus b3, coxsackievirus b2, coxsackievirus b6, coxsackievirus a16, and poliovirus 1. no proliferation against the cardiovirus encephalomyocarditis virus occurred. lymphocytes obtained from cord blood drawn from seven neonates were uniformly nonresponsive to enteroviral antigens. although serum neutralization antibody titers indicated ...	1990	1975596
secondary enterovirus infection in the murine model of myocarditis. pathologic and immunologic aspects.	enteroviruses are implicated as etiologic agents in the inflammatory diseases myocarditis and polymyositis. in this report, we show that a previous enterovirus exposure in mice can influence development of myocardial inflammation with a second enteroviral exposure. inoculation of 25-day-old male c3h/hej mice with 10(3) or 10(5) plaque-forming units (pfu) of infectious or ultra violet (uv)-inactivated coxsackievirus b2 (cvb2), followed by inoculation 28 days later with 10(5) pfu of a myocarditic ...	1990	2156432
effect of dexamethasone on coxsackievirus b2-infected rat beating heart cells in culture.	the effects of dexamethasone on cultured rat beating heart cells experimentally infected with coxsackievirus b2 were investigated at an early stage (1-3 days) post challenge. changes in the release of the cardiac enzyme, aspartate amino-transferase (ast), beating %, cytopathic effect (cpe), virus titre, ultrastructure and alterations of the electrical activity were evaluated. the protective effects of dexamethasone on infected cells were abolished except the release of ast at 5 days post infecti ...	1991	1655450
search for coxsackievirus b3 rna in idiopathic dilated cardiomyopathy using gene amplification by polymerase chain reaction.	a polymerase chain reaction (pcr) amplification assay was developed to detect coxsackievirus b3 ribonucleic acid (rna) in blood and myocardial tissue of explanted hearts from 40 patients who underwent cardiac transplantation and in 1 normal heart. twenty-one patients were affected by idiopathic dilated cardiomyopathy of different duration and 19 by coronary artery disease. coxsackievirus b3 in vitro infected vero cells and cells infected by related human enteroviruses (coxsackievirus b2, b4, and ...	1992	1311139
coxsackievirus b2 infection and aseptic meningitis: a focal outbreak among members of a high school football team.	in an investigation of a school outbreak of enterovirus-like illness and aseptic meningitis, an igm antibody assay was used to identify persons with evidence of recent coxsackie virus b2 infection. during september and october 1989, 81 (25%) of 319 students and staff reported an enterovirus-like illness; of these, 17 (21%) also had aseptic meningitis. attack rates for enterovirus-like illness were highest among varsity football team members (53%), and most of this illness occurred between 6 and ...	1993	8387565
enterovirus-induced myocarditis: hemodynamic deterioration with immunosuppressive therapy and successful application of interferon-alpha.	in 1985, myocarditis was diagnosed by endomyocardial biopsy in a 53-year-old woman who was free of symptoms of heart insufficiency. immunosuppressive therapy with azathioprine and prednisolone was prescribed as the patient had deteriorated to nyha ii heart insufficiency in march 1986. when application of immunosuppressive agents was terminated in 1987, serial endomyocardial biopsies revealed no signs of myocarditis, thus indicating effectiveness of immunosuppression. nevertheless, the patient ha ...	1994	8001305
acute infectious lymphocytosis caused by coxsackievirus b2.		1996	8970226
amplification and cloning of complete enterovirus genomes by long distance pcr.	a method for amplification and cloning of complete enterovirus cdna genomes is described. viral rna was reverse transcribed using an optimized protocol and a reverse transcriptase with reduced rnase h activity. amplicons corresponding to complete genomes of 14 prototype strains of group b coxsackieviruses and echoviruses were amplified using oligonucleotide primers derived from the coxsackievirus b3 genomic sequence of the 5' and 3' ends and a mixture of thermostable dna polymerases. coxsackievi ...	1997	9186942
three-step isolation method for sensitive detection of enterovirus, rotavirus, hepatitis a virus, and small round structured viruses in water samples.	control of drinking or bathing water quality in respect to viral contamination remains an unsolved problem. a highly sensitive isolation protocol was developed for concentration and detection of different enteric viruses from water samples. the three-step isolation procedure combines filtration with a positively charged nylon membrane, ultrafiltration and clean-up of the viral rna with a silica based membrane. detection of the viral rna is accomplished by reverse-transcription polymerase chain r ...	1997	9310854
enterovirus heart disease of adults: a persistent, limited organ infection in the presence of neutralizing antibodies.	detection of enterovirus rna in endomyocardial biopsies (emb) by reverse transcription/polymerase chain reaction (rt-pcr) is currently the preferred diagnostic procedure in suspected enterovirus heart disease (ehd), which can present clinically as myocarditis, dilated cardiomyopathy (dcm), and arrhythmogenic right ventricular cardiomyopathy (arvc). emb and peripheral blood mononuclear cells (pbmc) of 44 patients with suspected ehd were examined by nested rt-pcr to investigate whether the myocard ...	1997	9365882
antiviral activity of win 54954 in coxsackievirus b2 carrier state infected human myocardial fibroblasts.	persistent infections with a cardiotropic enterovirus, e.g. coxsackievirus b2 (cvb2), cause chronic myocarditis and eventually congestive heart failure. therefore, the antiviral activity of win 54954, a capsid binding antiviral agent that inhibits enterovirus uncoating, was studied in persistently cvb2-infected cultures of human myocardial fibroblasts. cultures displayed a typical carrier state infection with virus titers of 3.9 +/- 1.6 x 10(5) plaque forming units (pfu)/ml and 0.99% infected ce ...	1998	9497072
coxsackievirus b1 infection in infants less than 2 months of age.	most of the neonatal enteroviral infections reported in the literature are associated with coxsackievirus b2-b5 and echovirus 9 and 11. we report a retrospective coxsackievirus b1 (cb1) infection in infants less than 2 months of age. seventeen patients had aseptic meningitis and 8 had systemic sepsis (multi-organ involvement including meningitis, impaired liver function, and abnormality in coagulation). the symptoms and signs were nonspecific and could not be distinguished with bacterial infecti ...	1998	9572369
several different enterovirus serotypes can be associated with prediabetic autoimmune episodes and onset of overt iddm. childhood diabetes in finland (dime) study group.	in a prospective multicentre study described previously on prediabetic events in siblings of index cases with insulin-dependent diabetes mellitus, 31 children developed clinical diabetes during the observation period and 51 children seroconverted for islet cell antibodies or insulin autoantibodies. by using nonserotype specific eia and ria, it has shown recently that enterovirus infections in both groups were frequently associated with increases of islet cell antibody and/or insulin autoantibody ...	1998	9700636
genomic and phylogenetic characterization of coxsackievirus b2 prototype strain ohio-1.	the human picornavirus coxsackievirus b2 (cvb2) is often linked to several infections, from mild respiratory diseases to more severe illnesses such as myocarditis. in this study, we report the complete genome sequence of cvb2 prototype strain ohio-1. the genome sequence was determined from reverse transcribed viral rna, amplified with long distance pcr and used for non-radioactive sequencing. the full length pcr amplicons were used for in vitro transcription and the obtained crna was lipofected ...	1999	10082394
nucleotide sequences and mutations of the 5'-nontranslated region (5'ntr) of natural isolates of an epidemic echovirus 11' (prime).	an echovirus 11' (prime) virus caused an epidemic in hungary in 1989. the leading clinical form of the diseases was myocarditis. hemorrhagic hepatitis syndroms were also caused, however, with lethal outcome in 13 newborn babies. altogether 386 children suffered from registered clinical disease. no accumulation of serous meningitis cases and intrauterine death were observed during the epidemic, and the monovalent oral poliovirus vaccination campaign has prevented the further circulation of the vi ...	2000	11205106
genetic characterization of the coxsackievirus b2 3' untranslated region.	the secondary structure of the 3' untranslated region (3'utr) of picornaviruses is thought to be important for the initiation of negative-strand rna synthesis. in this study, genetic and biological analyses of the 3' terminus of coxsackievirus b2 (cvb2), which differs from other enteroviruses due to the presence of five additional nucleotides prior to the poly(a) tail, is reported. the importance of this extension was investigated using a 3'utr mutant lacking the five nucleotides prior to the po ...	2001	11369877
torsade de pointes tachycardia as a rare manifestation of acute enteroviral myocarditis.	a patient with cardiac arrest and documented torsade de pointes ventricular tachycardia is presented in whom acute coxsackievirus b2 myocarditis was identified as the most likely underlying cardiac condition. this case shows that torsade de pointes may occur as a rare manifestation of viral myocarditis. serial serological tests and endomyocardial biopsies may be helpful in establishing a diagnosis in such patients.	2001	11602535
dystrophin disruption in enterovirus-induced myocarditis and dilated cardiomyopathy: from bench to bedside.	genetic defects of the dystrophin-glycoprotein complex (dgc) cause hereditary dilated cardiomyopathy. enteroviruses can also cause cardiomyopathy and we have previously described a mechanism involved in enterovirus-induced dilated cardiomyopathy: the enteroviral protease 2a directly cleaves dystrophin in the hinge 3 region, leading to functional dystrophin impairment. during infection of mice with coxsackievirus b3, the dgc in the heart is disrupted and the sarcolemmal integrity is lost in virus ...	2004	12920582
a sensitive and quantitative single-tube real-time reverse transcriptase-pcr for detection of enteroviral rna.	enteroviruses (evs) are significant human pathogens. rapid and sensitive diagnostic techniques are desirable.	2004	15125871
characterization of virus isolates by particle-associated nucleic acid pcr.	diagnostic virus isolation is still frequently used, particularly from respiratory tract secretions. testing positive virus cultures for all possible viruses is time-consuming, and unexpected or unknown viruses may escape detection. therefore, a novel random pcr approach was developed that allows sequence-independent amplification of viral nucleic acids from virus isolation-positive cultures. selectivity for viral sequences is obtained by preferential isolation of nucleic acids that are particle ...	2005	15695669
cytolytic replication of coxsackievirus b2 in car-deficient rhabdomyosarcoma cells.	the six coxsackievirus b serotypes (cvb1-6) use the coxsackie- and adenovirus receptor (car) for host cell entry. four of these serotypes, cvb1, 3, 5 and 6, have also shown the capacity to replicate and cause cytolysis in rhabdomyosarcoma (rd) cells, a car-deficient cell line. this extended tropism has been associated with an acquired ability to bind decay accelerating factor (daf). in this study, we have adapted the cvb2 prototype strain ohio-1 (cvb2/o) to replicate in rd cells. two types of in ...	2005	15964091
histopathologic, immunohistochemical, and polymerase chain reaction assays in the study of cases with fatal sporadic myocarditis.	paraffin tissue blocks from 27 cases with sporadic myocarditis were collected during a 12-year period at a single medical examiner's office. blocks were studied by using histopathology; immunohistochemistry for viruses (adenovirus, enterovirus, influenza a and b, and human herpes types 4 and 5), bacteria (neisseria meningitidis, ehrlichia sp, spotted fever group rickettsia) and parasites (toxoplasma gondii and trypanosoma cruzi); and polymerase chain reaction (pcr)/rt-pcr for adenovirus and ente ...	2007	17602724
active coxsackieviral b infection is associated with disruption of dystrophin in endomyocardial tissue of patients who died suddenly of acute myocardial infarction.	in this study, we evaluated the potential direct role of enterovirus (ev) cardiac infections in the pathogenesis of myocardial infarction (mi).	2007	18061067
5' terminal deletions in the genome of a coxsackievirus b2 strain occurred naturally in human heart.	enteroviruses can induce human myocarditis, which can be modeled in mice inoculated with group b coxsackieviruses (cvb) and in which cvb evolve to produce defective, terminally deleted genomes. the 5' non-translated region (ntr) was enzymatically amplified from heart tissue of a fatal case of enterovirus-associated myocarditis in japan in 2002. while no intact 5' viral genomic termini were detected, 5' terminal deletions ranged in size from 22 to 36 nucleotides. sequence of the 5' third of this ...	2008	18378272
[rapid enterovirus genotyping in cerebrospinal fluids: a two-year prospective study in a virology laboratory setting].	enterovirus (ev - 68 serotypes) infections comprise a wide spectrum of clinical presentations including infections of the central nervous system. in severe clinical presentation or epidemics, the precise identification of the involved serotype is necessary.	2008	18835107
sewage workers: risk of acquiring enteric virus infections including hepatitis a.	to determine if sewage workers have an increased risk of acquiring viral infections, 66 workers at a small wastewater plant in north-eastern italy and 72 control subjects recruited from blood donors were enrolled in a seroprevalence study to determine whether sewage workers are at increased risk of acquiring viral infections. in order to evaluate various risk factors, a questionnaire was filled out by each worker whereas seropositivity to hepatitis a virus, coxsackievirus b2 - b3 - b4 - b5, and ...	2008	18843887
annual report of the australian national poliovirus reference laboratory, 2008.	the australian national poliovirus reference laboratory (nprl) is accredited by the world health organization (who) for the testing of stool specimens from cases of acute flaccid paralysis (afp), a major clinical presentation of poliovirus infection. the nprl, in collaboration with the australian paediatric surveillance unit, co-ordinates surveillance for cases of afp in children in australia, according to criteria recommended by the who. clinical specimens are referred from afp cases in childre ...	2009	20043599
a single coxsackievirus b2 capsid residue controls cytolysis and apoptosis in rhabdomyosarcoma cells.	coxsackievirus b2 (cvb2), one of six human pathogens of the group b coxsackieviruses within the enterovirus genus of picornaviridae, causes a wide spectrum of human diseases ranging from mild upper respiratory illnesses to myocarditis and meningitis. the cvb2 prototype strain ohio-1 (cvb2o) was originally isolated from a patient with summer grippe in the 1950s. later on, cvb2o was adapted to cytolytic replication in rhabdomyosarcoma (rd) cells. here, we present analyses of the correlation betwee ...	2010	20375176
an echovirus 18 associated outbreak of aseptic meningitis in taiwan:epidemiology, diagnostic, and genetic aspects.	in 2006, an outbreak of aseptic meningitis was noted in taiwan. the majority (27.4%) of enterovirus isolates was echovirus 18 (e18). from january to october, 2006, a total of 3,283 collected from patients with viral infection, including 173 cerebrospinal fluid (csf), were examined for viral isolation and identification. phylogenetic relationship among e18 strains was probed. the epidemic saw 339 enterovirus (ev) positive cases were identified by viral culture: e.g., echovirus 18 (e18) (27.4%, 93 ...	2011	21546563
molecular typing and epidemiology of enteroviruses in cyprus, 2003-2007.	human enteroviruses (hevs) are responsible for a wide spectrum of clinical diseases. even though usually associated with non-specific febrile illness, they are the most common cause of viral meningitis, pose a serious public health problem, especially during outbreaks. rapid detection and identification of hev serotypes, in clinical specimens is important in appropriate patients management and epidemiological investigation. a 5-year study (2003-2007) of clinical specimens from patients with vira ...	2011	21596905
the 3a protein from multiple picornaviruses utilizes the golgi adaptor protein acbd3 to recruit pi4kiiiβ	the activity of phosphatidylinositol 4-kinase class iii beta (pi4kiiiβ) has been shown to be required for the replication of multiple picornaviruses, however it is unclear whether a physical association between pi4kiiiβ and the viral replication machinery exists and if it does, whether association is necessary. we examined the ability of the 3a protein from 18 different picornaviruses to form a complex with pi4kiiiβ by affinity purification of strep-tagged transiently transfected constructs foll ...	2012	22258260
accuracy of diagnostic methods and surveillance sensitivity for human enterovirus, south korea, 1999-2011.	the epidemiology of enteroviral infection in south korea during 1999-2011 chronicles nationwide outbreaks and changing detection and subtyping methods used over the 13-year period. of 14,657 patients whose samples were tested, 4,762 (32.5%) samples were positive for human enterovirus (human ev); as diagnostic methods improved, the rate of positive results increased. a seasonal trend of outbreaks was documented. genotypes enterovirus 71, echovirus 30, coxsackievirus b5, enterovirus 6, and coxsack ...	2013	23876671
the antiviral effect of 7-hydroxyisoflavone against enterovirus 71 in vitro.	enterovirus 71 (ev71) is the major causative agent of hand foot and mouth disease. and ev71 causes epidemics worldwide, particularly in the asia-pacific region. unfortunately, currently there is no approved vaccine or antiviral drug for ev71-induced disease prevention and therapy. in screening for anti-ev71 candidates, we found that 7-hydroxyisoflavone was active against ev71. 7-hydroxyisoflavone exhibited strong antiviral activity against three different ev71 strains. the 50% inhibitory concent ...	2013	23464760
new alloferon analogues: synthesis and antiviral properties.	we have extended our study on structure/activity relationship studies of insect peptide alloferon (h-his-gly-val-ser-gly-his-gly-gln-his-gly-val-his-gly-oh) by evaluating the antiviral effects of new alloferon analogues. we synthesized 18 alloferon analogues: 12 peptides with sequences shortened from n- or c-terminus and 6 n-terminally modified analogues h-x(1)-gly-val-ser-gly-his-gly-gln-his-gly-val-his-gly-oh, where x(1) = phe (13), tyr (14), trp (15), phg (16), phe(p-cl) (17), and phe(p-ome) ...	2013	22883213
distinct systemic and central nervous system disease patterns in enterovirus and parechovirus infected children.	enteroviruses (ev) and human parechoviruses (hpev) infections are increasingly identified in neonates and young children with sepsis, meningitis and encephalitis. we investigated ev and hpev viral loads in plasma and cerebrospinal fluid (csf) among those presenting with sepsis or central nervous system (cns) disease to gain understanding of the nature of these infections.	2014	24662038
non-rhinovirus enteroviruses associated with respiratory infections in peru (2005-2010).	enteroviruses (evs) are a common cause of respiratory tract infections and are classified into seven species (eva-d and rhinoviruses [rhvs] a-c) with more than 200 different serotypes. little is known about the role of non-rhv evs in respiratory infections in south america. the aim of this study was to describe the epidemiology of non-rhv evs detected in patients with influenza-like illness enrolled in a passive surveillance network in peru.	2014	25244984
high incidence of mammalian orthoreovirus identified by environmental surveillance in taiwan.	wild poliovirus (wpv) persists in diverse locales worldwide, spreading outward from endemic areas. in response to the international threat of wpv transmission and changes in the national vaccination policy, we established an environmental surveillance system to monitor the circulation of wild and vaccine-related poliovirus in taiwan. from july 2012 to december 2013, we collected sewage specimens every month from 10 sewage treatment plants located throughout taiwan. the specimens were concentrate ...	2015	26555962
phylodynamic reconstruction of the spatiotemporal transmission and demographic history of coxsackievirus b2.	studies regarding coxsackievirus (cv) tend to focus on epidemic outbreaks, an imbalanced topology is considered to be an indication of acute infection with partial cross-immunity. in enteroviruses, a clear understanding of the characteristics of tree topology, transmission, and its demographic dynamics in viral succession and circulation are essential for identifying prevalence trends in endemic pathogens such as coxsackievirus b2 (cv-b2). this study applied a novel bayesian evolutionary approac ...	2015	26390997
formononetin inhibits enterovirus 71 replication by regulating cox- 2/pge₂ expression.	the activation of erk, p38 and jnk signal cascade in host cells has been demonstrated to up-regulate of enterovirus 71 (ev71)-induced cyclooxygenase-2 (cox-2)/ prostaglandins e2 (pge₂) expression which is essential for viral replication. so, we want to know whether a compound can inhibit ev71 infection by suppressing cox-2/pge₂ expression.	2015	25890183
genotyping of enteroviruses isolated in kenya from pediatric patients using partial vp1 region.	enteroviruses (ev) are responsible for a wide range of clinical diseases in humans. though studied broadly in several regions of the world, the genetic diversity of human enteroviruses (hev) circulating in the sub-saharan africa remains under-documented. in the current study, we molecularly typed 61 hev strains isolated in kenya between 2008 and 2011 targeting the 3'-end of the vp1 gene. viral rna was extracted from the archived isolates and part of the vp1 gene amplified by rt-pcr, followed by ...	2016	27026855
viral persistence in surface and drinking water: suitability of pcr pre-treatment with intercalating dyes.	after many outbreaks of enteric virus associated with consumption of drinking water, the study of enteric viruses in water has increased significantly in recent years. in order to better understand the dynamics of enteric viruses in environmental water and the associated viral risk, it is necessary to estimate viral persistence in different conditions. in this study, two representative models of human enteric viruses, adenovirus 41 (adv 41) and coxsackievirus b2 (cv-b2), were used to evaluate th ...	2016	26773484
molecular epidemiological study of enteroviruses associated with encephalitis in children from hangzhou, china.	enterovirus (ev) has over 100 serotypes of species a-d, which can cause various symptoms in infants. enterovirus encephalitis (eve) is serve disease with high morbidity and mortality in children. to well define the epidemiology of eve, we wanted to know more about ev and ev molecular typing by conducting this study in hangzhou.cerebrospinal fluid samples were collected from children with diagnosis of encephalitis. meanwhile, one-step real-time rt-pcr was used for the detection of ev, and we also ...	2016	27893696
the transcriptome of rhabdomyosarcoma cells infected with cytolytic and non-cytolytic variants of coxsackievirus b2 ohio-1.	the transcriptomes of cells infected with lytic and non-lytic variants of coxsackievirus b2 ohio-1 (cvb2o) were analyzed using next generation sequencing. this approach was selected with the purpose of elucidating the effects of lytic and non-lytic viruses on host cell transcription. total rna was extracted from infected cells and sequenced. the resulting reads were subsequently mapped against the human and cvb2o genomes. the amount of intracellular rna was measured, indicating lower proportions ...	2016	27760161
molecular epidemiological study of enteroviruses associated with encephalitis in children from hangzhou, china.	enterovirus (ev) has over 100 serotypes of species a-d, which can cause various symptoms in infants. enterovirus encephalitis (eve) is serve disease with high morbidity and mortality in children. to well define the epidemiology of eve, we wanted to know more about ev and ev molecular typing by conducting this study in hangzhou.cerebrospinal fluid samples were collected from children with diagnosis of encephalitis. meanwhile, one-step real-time rt-pcr was used for the detection of ev, and we also ...	2016	27749541
molecular characterization of a new human coxsackievirus b2 associated with severe hand-foot-mouth disease in yunnan province of china in 2012.	human coxsackievirus b2 (cvb2) belongs to the species human enterovirus b and can cause aseptic meningitis, myocarditis and hand-foot-mouth disease (hfmd). we first determined the complete genome of the rw41-2/yn/chn/2012 strain, isolated from a patient with hfmd and aseptic meningitis in the yunnan province, china in 2012. the strain shared 83.5 % and 82.2 % nucleotide similarity with cvb2 prototype strain ohio-1, in the complete vp1 gene and the complete genome, respectively. using phylogeneti ...	2017	27709402
pathogenesis of coxsackievirus b2 in mice: characterisation of clinical isolates of the coxsackievirus b2 from patients with myocarditis and aseptic meningitis in korea.	group b coxsackieviruses (cvbs) are a group of common human pathogens causing various clinical symptoms. although the virology of cvb is well known, there is limited information on viral pathogenesis and the relationship between clinical symptoms and viral phenotype, particularly for cvb type 2 (cvb2). in this study, two cvb2 strains were isolated: cb2/04/279 from a stool of an acute myocarditis patient with heart failure; and cb2/04/243 from an aseptic meningitis patient in 2004 in korea. a hig ...	2017	28384999