Publications

bloodstream infections due to trichosporon spp.: species distribution, trichosporon asahii genotypes determined on the basis of ribosomal dna intergenic spacer 1 sequencing, and antifungal susceptibility testing.	the reevaluation of the genus trichosporon has led to the replacement of the old taxon trichosporon beigelii by six new species. sequencing of the ribosomal dna (rdna) intergenic spacer 1 (igs1) is currently mandatory for accurate trichosporon identification, but it is not usually performed in routine laboratories. here we describe trichosporon species distribution and prevalence of trichosporon asahii genotypes based on rdna igs1 sequencing as well as antifungal susceptibility profiles of 22 is ...	2009	19225102
oil production on wastewaters after butanol fermentation by oleaginous yeast trichosporon coremiiforme.	from the distillation process after butanol fermentation, wastewaters mainly consisted of organic acids and residual sugars and with high cod (usually >20,000 mg/l) are generated. without any pretreatment and adding other nutrients (nitrogen sources and trace elements), these wastewaters were used as substrate for microbial oil production by oleaginous yeast trichosporon coremiiforme. after 5 days' lipid fermentation, all the sugars and organic acids measured were totally utilized by t. coremiif ...	2012	22704190
genome profiling (gp) as an effective tool for monitoring culture collections: a case study with trichosporon.	species identification and classification of a large number of microbes are essential and heavy workloads in culture collections and relevant laboratories. the identification of species usually requires different methods depending on species. therefore, the development of a method which is simple and applicable to any organisms will lessen the burdens, increase the reliability of databases and thus enhance the science on microbes. the genome profiling (gp) method, developed previously, was found ...	2012	22401825
dynamic study of yeast species and saccharomyces cerevisiae strains during the spontaneous fermentations of muscat blanc in jingyang, china.	the evolution of yeast species and saccharomyces cerevisiae genotypes during spontaneous fermentations of muscat blanc planted in 1957 in jingyang region of china was followed in this study. using a combination of colony morphology on wallerstein nutrient (wln) medium, sequence analysis of the 26s rdna d1/d2 domain and 5.8s-its-rflp analysis, a total of 686 isolates were identified at the species level. the six species identified were s. cerevisiae, hanseniaspora uvarum, hanseniaspora opuntiae, ...	2013	23200649
sequence-based identification, genotyping and eucast antifungal susceptibilities of trichosporon clinical isolates from greece.	trichosporon yeasts constitute emerging pathogens, implicated in organ-specific and systemic infections. in this first, comprehensive study of trichosporon clinical isolates in greece, 42 isolates were identified by sequencing the hypervariable d1/d2 domain of the large subunit (lsu) rdna gene, while trichosporon asahii were genotyped by sequencing the intergenic spacer 1 region, and antifungal susceptibilities were determined by the edef 7.2 (eucast) method, in parallel with the clsi standard. ...	2014	24330082
carbon source utilization and inhibitor tolerance of 45 oleaginous yeast species.	conversion of lignocellulosic hydrolysates to lipids using oleaginous (high lipid) yeasts requires alignment of the hydrolysate composition with the characteristics of the yeast strain, including ability to utilize certain nutrients, ability to grow independently of costly nutrients such as vitamins, and ability to tolerate inhibitors. some combination of these characteristics may be present in wild strains. in this study, 48 oleaginous yeast strains belonging to 45 species were tested for abili ...	2014	24818698
yeast species and genetic heterogeneity within debaryomyces hansenii along the ripening process of traditional ewes' and goats' cheeses.	the yeasts present during the ripening process of ewes' and goats' cheeses produced in a small traditional dairy in mediterranean spain were isolated and identified. five hundred and thirty strains pertaining to eleven yeast species representing eight genera were identified using molecular methods. debaryomyces hansenii was the yeast species most frequently isolated in all cheeses. other yeast species commonly found in dairy products were present at the first maturing weeks. two yeast species tr ...	2014	24290639
gene cloning, high-level expression, and characterization of an alkaline and thermostable lipase from trichosporon coremiiforme v3.	the present study describes the gene cloning and high-level expression of an alkaline and thermostable lipase gene from trichosporon coremiiforme v3. nucleotide analysis revealed that this lipase gene has an open reading frame of 1,692 bp without any introns, encoding a protein of 563 amino acid residues. the lipase gene without its signal sequence was cloned into plasmid ppiczαa and overexpressed in pichia pastoris x33. the maximum lipase activity of recombinant lipase was 5,000 u/ml, which was ...	2015	25588556