Publications

helical flagellation in centipeda periodontii, a gram-negative, anaerobic bacillus from periodontitis lesions.	the insertion pattern of flagellation of centipeda periodontii was determined from electron micrographs of negatively stained cells treated with various detergents to remove their outer cell wall layer(s) selectively. the best results were obtained with 0.2% sodium dodecyl sulphate and a treatment time of 1-2 min. detergent-treated cells displayed a unique helical pattern of flagellation which we designate as helicotrichous (gk n. helix, helix + gk n. thrix, hair). flagella originated from an el ...	1984	6707608
immunosuppressive effects of centipeda periodontii: selective cytotoxicity for lymphocytes and monocytes.	we have examined soluble sonic extracts prepared from several strains of centipeda periodontii for their ability to alter human lymphocyte function. these organisms were isolated from subgingival plaque of patients with periodontal disease. we found that sonicates from several, but not all, strains of c. periodontii caused a dose-dependent inhibition of lymphocyte responsiveness to concanavalin a, phytohemagglutinin, pokeweed mitogen, and formalinized staphylococcus aureus. inhibition was associ ...	1987	3653981
ultrastructure of the flagellar basal body complex of centipeda periodontii.	the morphology and insertion of the flagellar basal body complex into the cell wall of centipeda periodontii was studied by electron microscopy of both negatively and positively stained specimens. freshly harvested cells were examined either after treatment with 0.2% sodium dodecyl sulfate (sds) for 2 min and negatively stained with phosphotungstic acid, or after treatment according to standard electron microscopy procedures that included positive staining. small numbers of flagella were disloca ...	1988	3171249
isolation and characterization of lipopolysaccharide from centipeda periodontii atcc 35019.	lipopolysaccharide (lps) was extracted from cells of centipeda periodontii atcc 35019 by a hot phenol-water method, purified by nuclease treatment and by repeated ultracentrifugations. the lps was composed of 21.7% hexose, 9.1% hexosamine, 10.3% fatty acid, 0.5% protein, 2.5% phosphorus, 4.3% 2-keto-3-deoxyoctonate(kdo) and 2.3% heptose. the major fatty acids of the lps were 3oh c13:0 and c13:0. c. periodontii lps was able to gelatinize limulus amebocyte and to produce the local schwartzman reac ...	1990	2087348
identification and analyses of periodontal pathogens in taiwan by microbiological tests.	the purpose of this study was to use microbiological tests for diagnosis of periodontal diseases in taiwan. anaerobic culture, direct microscopy, indirect immunofluorescence (if), and biochemical tests were used to examine 336 samples for the specific microorganisms in subgingival plaque. the results indicated that gram-negative species and motile bacteria were less frequently detected, and in lower proportion, in samples from healthy sites. the bacteria found frequently in healthy group were th ...	1994	9747334
phylogenetic characterization of centipeda periodontii, selenomonas sputigena and selenomonas species by 16s rrna gene sequence analysis.	the nearly complete 16s rrna gene sequences for oral gram-negative anaerobic motile bacteria, centipeda periodontii, selenomonas sputigena and selenomonas species (formerly s. sputigena type strain), were determined in order to unveil their relationship to other oral motile bacteria. to determine the phylogenetic characterization of these bacteria, their 16s rrna gene sequences were obtained and compared with those from the ribosomal sequence databases previously reported. the 16s rrna gene sequ ...	1999	10464949
development of 16s rdna-based pcr assay for detecting centipeda periodontii and selenomonas sputigena.	to detect oral motile bacteria directly from dental plaque, specific pcr primers for centipeda periodontii and selenomonas sputigena were designed based on the sequence analysis of their 16s rdna. the primers were specific and sensitive enough to amplify dna fragments from the available oral bacteria. the detection limit was fewer than 10 bacterial cells per sample. it was also possible to detect these bacteria in dental plaque. the prevalence of these bacteria varied in each sample. the specifi ...	2000	10849269
anaerobic bacteria cultured from the tongue dorsum of subjects with oral malodor.	the bacteria on the dorsum of the tongue are the most frequent cause of oral malodor; however, the bacterial flora of the tongue has not been well defined. although recent studies have used dna probes to detect the presence of certain periodontal pathogens, cultural studies have been limited because of the complexity of the flora of the tongue dorsum. the purpose of this study was to grow and to identify maximum numbers of capnophylic gram-negative bacilli and anaerobic micro-organisms by cultur ...	2003	16887710
nested pcr detection of centipeda periodontii in primary endodontic infections.	in recent years, molecular genetic methodologies have provided significant additional knowledge about components of the microbiota associated with infections of endodontic origin. following this research line, the purpose of the present study was to investigate the prevalence of centipeda periodontii in primary endodontic infections using a species-specific nested pcr assay. samples were collected from fifty teeth having carious lesions, necrotic pulps, and different forms of periradicular disea ...	2004	15055428
analysis of bacterial flora associated with peri-implantitis using obligate anaerobic culture technique and 16s rdna gene sequence.	to analyze and characterize the predominant bacterial flora associated with peri-implantitis by using culture techniques under obligate anaerobic conditions and 16s rdna gene sequences.	2013	24278920
centipeda periodontii in human periodontitis.	this study assessed the subgingival occurrence of the flagellated, gram-negative, anaerobic rod centipeda periodontii in chronic periodontitis and periodontal health/gingivitis with species-specific nucleic acid probes, and evaluated the in vitro resistance of subgingival isolates to therapeutic levels of amoxicillin, metronidazole, and doxycycline. subgingival plaque biofilm specimens from 307 adults with chronic periodontitis, and 48 adults with periodontal health/localized gingivitis, were ev ...	2015	25037463