Publications

transfer of several phytopathogenic pseudomonas species to acidovorax as acidovorax avenae subsp. avenae subsp. nov., comb. nov., acidovorax avenae subsp. citrulli, acidovorax avenae subsp. cattleyae, and acidovorax konjaci.	dna-rrna hybridizations, dna-dna hybridizations, polyacrylamide gel electrophoresis of whole-cell proteins, and a numerical analysis of carbon assimilation tests were carried out to determine the relationships among the phylogenetically misnamed phytopathogenic taxa pseudomonas avenae, pseudomonas rubrilineans, "pseudomonas setariae," pseudomonas cattleyae, pseudomonas pseudoalcaligenes subsp. citrulli, and pseudomonas pseudoalcaligenes subsp. konjaci. these organisms are all members of the fami ...	1992	1371056
investigating intraspecific variation of acidovorax avenae subsp. citrulli using dna fingerprinting and whole cell fatty acid analysis.	abstract to assess the diversity of acidovorax avenae subsp. citrulli, 121 strains from watermelon, cantaloupe, and pumpkin were compared using pulse field gel electrophoresis of spei-digested dna and gas chromatographic analysis of fatty acid methyl esters. twenty-nine unique dna fragments resulted from dna digestion, and 14 distinct haplotypes were observed. based on cluster analysis, two subgroups, i and ii, were recognized, which accounted for 84.8% (eight haplotypes) and 15.2% (six haplotyp ...	2000	18944608
role of blossoms in watermelon seed infestation by acidovorax avenae subsp. citrulli.	abstract the role of watermelon blossom inoculation in seed infestation by acidovorax avenae subsp. citrulli was investigated. approximately 98% (84/87) of fruit developed from blossoms inoculated with 1 x 10(7) or 1 x 10(9) cfu of a. avenae subsp. citrulli per blossom were asymptomatic. using immunomagnetic separation and the polymerase chain reaction, a. avenae subsp. citrulli was detected in 44% of the seed lots assayed, despite the lack of fruit symptoms. furthermore, viable colonies were re ...	2003	18942974
biological control to protect watermelon blossoms and seed from infection by acidovorax avenae subsp. citrulli.	abstract the efficacy of biological control seed treatments with pseudomonas fluorescens (a506), acidovorax avenae subsp. avenae (aaa 99-2), and an unidentified gram-positive bacterium recovered from watermelon seed (ws-1) was evaluated for the management of bacterial fruit blotch (bfb) of watermelon. in growth chamber and greenhouse experiments, seed treated with aaa 99-2 displayed superior disease suppression, reducing bfb transmission by 96.5%. aaa 99-2, p. fluorescens a506, and kocide also s ...	2005	18943044
simultaneous detection of acidovorax avenae subsp. citrulli and didymella bryoniae in cucurbit seedlots using magnetic capture hybridization and real-time polymerase chain reaction.	to improve the simultaneous detection of two pathogens in cucurbit seed, a combination of magnetic capture hybridization (mch) and multiplex real-time polymerase chain reaction (pcr) was developed. single-stranded dna hybridization capture probes targeting dna of acidovorax avenae subsp. citrulli, causal agent of bacterial fruit blotch, and didymella bryoniae, causal agent of gummy stem blight, were covalently attached to magnetic particles and used to selectively concentrate template dna from c ...	2009	19453225
type iv pili are required for virulence, twitching motility, and biofilm formation of acidovorax avenae subsp. citrulli.	acidovorax avenae subsp. citrulli is the causal agent of bacterial fruit blotch (bfb), a threatening disease of watermelon, melon, and other cucurbits. despite the economic importance of bfb, relatively little is known about basic aspects of the pathogen's biology and the molecular basis of its interaction with host plants. to identify a. avenae subsp. citrulli genes associated with pathogenicity, we generated a transposon (tn5) mutant library on the background of strain m6, a group i strain of ...	2009	19589067
multilocus sequence typing reveals two evolutionary lineages of acidovorax avenae subsp. citrulli.	acidovorax avenae subsp. citrulli, causal agent of bacterial fruit blotch, has caused considerable damage to the watermelon and melon industry in china and the united states. understanding the emergence and spread of this pathogen is important for controlling the disease. to build a fingerprinting database for reliable identification and tracking of strains of a. avenae subsp. citrulli, a multilocus sequence typing (mlst) scheme was developed using seven conserved loci. the study included 8 orig ...	2009	19594310
pamdb, a multilocus sequence typing and analysis database and website for plant-associated microbes.	although there are adequate dna sequence differences among plant-associated and plant-pathogenic bacteria to facilitate molecular approaches for their identification, identification at a taxonomic level that is predictive of their phenotype is a challenge. the problem is the absence of a taxonomy that describes genetic variation at a biologically relevant resolution and of a database containing reference strains for comparison. moreover, molecular evolution, population genetics, ecology, and epi ...	2010	20128693
assessing adhesion, biofilm formation and motility of acidovorax citrulli using microfluidic flow chambers.	acidovorax citrulli is the causal agent of bacterial fruit blotch of cucurbits. we have shown previously that type iv pili (tfp) are required for wild-type levels of virulence of a. citrulli on melon and that this pathogen can colonize and move thorough the xylem vessels of host seedlings. here, comparative studies between wild-type and tfp mutant strains using microfluidic flow chambers demonstrated that tfp play a critical role in both the surface attachment and the biofilm formation of a. cit ...	2010	20807236
development of surface plasmon resonance imaging for detection of acidovorax avenae subsp. citrulli (aac) using specific monoclonal antibody.	an immunosensor based on surface plasmon resonance imaging (spr imaging) using a specific monoclonal antibody 11e5 (mab 11e5) was developed for the detection of the seed-borne bacterium acidovorax avenae subsp. citrulli (aac), which causes fruit blotch in watermelons and cantaloupes, and compared to the conventional elisa technique. the 1:40 mixed self-assembled monolayer (mixed sam) surface was used for the immobilized mab 11e5 on sensor surface for the detection of aac. both whole cells and br ...	2010	21093249
rapid on-site detection of acidovorax avenae subsp. citrulli by gold-labeled dna strip sensor.	acidovorax avenae subsp. citrulli (aac) is one of the most harmful diseases in cucurbit production. a rapid and sensitive dna strip sensor was constructed based on gold nanoparticle-labeled oligonucleotide probes for the detection of aac. both the qualitative and semi-quantitative detections of target dna were successfully achieved using the developed dna strip sensor. the qualitative limit of detection (lod) of the strip sensor was determined as 4nm. the lod for the semi-quantitative detection ...	2011	21536424
the cucurbit pathogenic bacterium acidovorax citrulli requires a polar flagellum for full virulence before and after host tissue penetration.	acidovorax citrulli causes seedling blight and bacterial fruit blotch of cucurbits. previous reports demonstrated the contribution of type iv pili (tfp) to a. citrulli virulence and to systemic infection of melon seedlings. microfluidic flow chamber assays demonstrated the involvement of tfp in surface adhesion and biofilm formation, whereas polar flagella did not appear to contribute to either of these features. on the other hand, a transposon mutant impaired in the biosynthesis of polar flagel ...	2011	21554180
[relationship of histidine auxotrophy of acidovorax citrulli with pathogenicity].	acidovorax citrulli (ac) is an important bacterium that occurs in watermelon, melon and other cucurbits. it mainly damages watermelon and melon, and can cause leaf blight, fruit rot, and even mortality.	2011	22126073
differentiation in maldi-tof ms and ftir spectra between two closely related species acidovorax oryzae and acidovorax citrulli.	two important plant pathogenic bacteria acidovorax oryzae and acidovorax citrulli are closely related and often not easy to be differentiated from each other, which often resulted in a false identification between them based on traditional methods such as carbon source utilization profile, fatty acid methyl esters, and elisa detection tests. maldi-tof ms and fourier transform infrared (ftir) spectra have recently been successfully applied in bacterial identification and classification, which pro ...	2012	22900823
acidovorax citrulli: generating basic and applied knowledge to tackle a global threat to the cucurbit industry.	acidovorax citrulli is the causal agent of bacterial fruit blotch (bfb) of cucurbit plants. in recent years, the disease has spread to many parts of the world, mainly via the inadvertent distribution of contaminated commercial seeds. because of the costly lawsuits filed by growers against seed companies and the lack of efficient management methods, bfb represents a serious threat to the cucurbit industry, and primarily to watermelons and melons. despite the economic importance of the disease, li ...	2012	22738439
rapid on-site detection of acidovorax citrulli by cross-priming amplification.	cross-priming amplification (cpa) for acidovorax citrulli detection was evaluated in this study. the sensitivity of cpa assay for pure bacterial culture was 3.7 × 10(3) cfu/ml. bacteria on naturally infected watermelon seeds were detected using cpa assay, suggesting this method is suitable for a. citrulli on-site detection from watermelon seeds.	2012	22507851
location of acidovorax citrulli in infested watermelon seeds is influenced by the pathway of bacterial invasion.	watermelon seeds can become infested by acidovorax citrulli, the causal agent of bacterial fruit blotch (bfb) of cucurbits via penetration of the ovary pericarp or by invasion of the pistil. this study investigated the effect of these invasion pathways on a. citrulli localization in seeds. seed samples (n = 20 or 50 seeds/lot) from pistil- and pericarp-inoculated lots were dissected into testa, perisperm-endosperm (pe) layer, and embryo tissues and tested for a. citrulli by species-specific poly ...	2012	22352307
implementation of microfluidic sandwich elisa for superior detection of plant pathogens.	rapid and economical screening of plant pathogens is a high-priority need in the seed industry. crop quality control and disease surveillance demand early and accurate detection in addition to robustness, scalability, and cost efficiency typically required for selective breeding and certification programs. compared to conventional bench-top detection techniques routinely employed, a microfluidic-based approach offers unique benefits to address these needs simultaneously. to our knowledge, this w ...	2013	24376668
phenotypic variation in the plant pathogenic bacterium acidovorax citrulli.	acidovorax citrulli causes bacterial fruit blotch (bfb) of cucurbits, a disease that threatens the cucurbit industry worldwide. despite the economic importance of bfb, little is known about pathogenicity and fitness strategies of the bacterium. we have observed the phenomenon of phenotypic variation in a. citrulli. here we report the characterization of phenotypic variants (pvs) of two strains, m6 and 7a1, isolated from melon and watermelon, respectively. phenotypic variation was observed follow ...	2013	24023830
multiplex detection of plant pathogens using a microsphere immunoassay technology.	plant pathogens are a serious problem for seed export, plant disease control and plant quarantine. rapid and accurate screening tests are urgently required to protect and prevent plant diseases spreading worldwide. a novel multiplex detection method was developed based on microsphere immunoassays to simultaneously detect four important plant pathogens: a fruit blotch bacterium acidovorax avenae subsp. citrulli (aac), chilli vein-banding mottle virus (cvbmv, potyvirus), watermelon silver mottle v ...	2013	23638044
1.37 å crystal structure of pathogenic factor pectate lyase from acidovorax citrulli.	pectates lyase (pel) plays an important role in bacteria pathogenicity. the crystal structure of pel from acidovorax citrulli (acpel) has been solved to 1.37 å resolution. acpel belongs to the polysaccharide lyase family 1 (pl1), which has a characteristic right-handed β-helix fold. acpel is similar with other pels in the pl1 family, but also shows some differences at the substrate binding site.	2013	23568384
effect of chitosan solution on the inhibition of acidovorax citrulli causing bacterial fruit blotch of watermelon.	the production of watermelon in china has been seriously hampered by fruit blotch disease and limited control measures are now applied. chitosan has been employed to control a variety of plant diseases and is considered to be the most promising biochemical to control this disease.	2013	23400827
comparative analysis of type iii secreted effector genes reflects divergence of acidovorax citrulli strains into three distinct lineages.	acidovorax citrulli causes bacterial fruit blotch of cucurbits, a serious economic threat to watermelon (citrullus lanatus) and melon (cucumis melo) production worldwide. based on genetic and biochemical traits, a. citrulli strains have been divided into two distinct groups: group i strains have been mainly isolated from various non-watermelon hosts, while group ii strains have been generally isolated from and are highly virulent on watermelon. the pathogen depends on a functional type iii secre ...	2014	24848275
interactions of seedborne bacterial pathogens with host and non-host plants in relation to seed infestation and seedling transmission.	the ability of seed-borne bacterial pathogens (acidovorax citrulli, clavibacter michiganensis subsp. michiganensis, pseudomonas syringae pv. tomato, xanthomonas euvesicatoria, and pseudomonas syringae pv. glycinea) to infest seeds of host and non-host plants (watermelon, tomato, pepper, and soybean) and subsequent pathogen transmission to seedlings was investigated. a non-pathogenic, pigmented strain of serratia marcescens was also included to assess a null-interacting situation with the same pl ...	2014	24936863
role of blossom colonization in pepper seed infestation by xanthomonas euvesicatoria.	colonization of xanthomonas euvesicatoria was investigated in pepper blossoms and the relationship between inoculum concentrations and seed infestation was determined. inoculation of blossoms resulted in asymptomatic pepper fruit. however, real-time polymerase chain reaction detected x. euvesicatoria in 39% of the seed lots assayed and viable colonies were recovered from 35% of them. successful transmission occurred in 16% of the seed lots tested. in a separate experiment, x. euvesicatoria reach ...	2014	24111576
antibody array in a multiwell plate format for the sensitive and multiplexed detection of important plant pathogens.	the global seed market is considered to be an important industry with a total value of $10,543 million us dollars in 2012. because plant pathogens such as bacteria and viruses cause a significant economic loss to both producers and exporters, the seed export industry urgently requires rapid, sensitive, and inexpensive testing for the pathogens to prevent disease spreading worldwide. this study developed an antibody array in a multiwell plate format to simultaneously detect four crucial plant pat ...	2014	24945525
the type vi protein secretion system contributes to biofilm formation and seed-to-seedling transmission of acidovorax citrulli on melon.	the type vi protein secretion system (t6ss) is essential for the virulence of several gram-negative bacteria. in this study, we identified a t6ss gene cluster in acidovorax citrulli, a plant-pathogenic bacterium that causes bacterial fruit blotch (bfb) of cucurbits. one t6ss cluster, of approximately 25 kb in length and comprising 17 genes, was found in the a. citrulli aac00-1 genome. seventeen a. citrulli mutants were generated, each with a deletion of a single t6ss core gene. there were signif ...	2015	24863458
identification of pathogenicity-related genes in biofilm-defective acidovorax citrulli by transposon tn5 mutagenesis.	biofilm formation is important for virulence of a large number of plant pathogenic bacteria. indeed, some virulence genes have been found to be involved in the formation of biofilm in bacterial fruit blotch pathogen acidovorax citrulli. however, some virulent strains of a. citrulli were unable to format biofilm, indicating the complexity between biofilm formation and virulence. in this study, virulence-related genes were identified in the biofilm-defective strain a1 of a. citrulli by using tn5 i ...	2015	26602922
rapid and specific detection of acidovorax avenae subsp. citrulli using sybr green-based real-time pcr amplification of the yd-repeat protein gene.	the aim of this study was to develop a sybr green-based real-time pcr assay for the rapid, specific, and sensitive detection of acidovorax avenae subsp. citrulli, which causes bacterial fruit blotch (bfb), a serious disease of cucurbit plants. the molecular and serological methods currently available for the detection of this pathogen are insufficiently sensitive and specific. thus, a novel sybr green-based real-time pcr assay targeting the yd-repeat protein gene of a. avenae subsp. citrulli was ...	2015	25951847
genome sequence of acidovorax citrulli group 1 strain pslb65 causing bacterial fruit blotch of melons.	acidovorax citrulli is typed into two groups, mainly based on the host. we determined the draft genome of a. citrulli group 1 strain pslb65. the strain was isolated from melon collected from xinjiang province, china. the a. citrulli pslb65 genome contains 4,903,443 bp and has a g+c content of 68.8 mol%.	2015	25908136
genome sequence of a copper-resistant strain of acidovorax citrulli causing bacterial fruit blotch of melons.	bacterial fruit blotch (bfb) of melons is a seed-borne disease caused by acidovorax citrulli. we determined the draft genome of a. citrulli tw6. the strain was isolated from a watermelon collected from beijing, china. the a. citrulli tw6 genome contains 5,080,614 bp and has a g+c content of 68.7 mol%.	2015	25908132
study on screening and antagonistic mechanisms of bacillus amyloliquefaciens 54 against bacterial fruit blotch (bfb) caused by acidovorax avenae subsp. citrulli.	bacterial fruit blotch (bfb) was a serious threat to cucurbitaceae crops. it was caused by the gram-negative bacterium acidovorax avenae subsp. citrulli. two hundred strains, which have the potential in controlling plant diseases in our laboratory's biocontrol strain library, were employed to this research to screen some antagonistic bacteria, which can efficiently control bacterial fruit blotch disease. based on the results of antagonistic activity experiments, greenhouse tests and field trials ...	2015	25267487
the vapb-vapc operon of acidovorax citrulli functions as a bona-fide toxin-antitoxin module.	toxin-antitoxin systems are commonly found on plasmids and chromosomes of bacteria and archaea. these systems appear as biscystronic genes encoding a stable toxin and a labile antitoxin, which protects the cells from the toxin's activity. under specific, mostly stressful conditions, the unstable antitoxin is degraded, the toxin becomes active and growth is arrested. using genome analysis we identified a putative toxin-antitoxin encoding system in the genome of the plant pathogen acidovorax citru ...	2015	26779154
bacterial cyanuric acid hydrolase for water treatment.	di- and trichloroisocyanuric acids are widely used as water disinfection agents, but cyanuric acid accumulates with repeated additions and must be removed to maintain free hypochlorite for disinfection. this study describes the development of methods for using a cyanuric acid-degrading enzyme contained within nonliving cells that were encapsulated within a porous silica matrix. initially, three different bacterial cyanuric acid hydrolases were compared: trzd from acidovorax citrulli strain 12227 ...	2015	26187963
selection of a protein solubilization method suitable for phytopathogenic bacteria: a proteomics approach.	finding the best extraction method of proteins from lysed cells is the key step for detection and identification in all proteomics applications. these are important to complement the knowledge about the mechanisms of interaction between plants and phytopathogens causing major economic losses. to develop an optimized extraction protocol, strains of acidovorax citrulli, pectobacterium carotovorum subsp. carotovorum and ralstonia solanacearum were used as representative cells in the study of phytop ...	2015	25670925
insights from the genome sequence of acidovorax citrulli m6, a group i strain of the causal agent of bacterial fruit blotch of cucurbits.	acidovorax citrulli is a seedborne bacterium that causes bacterial fruit blotch of cucurbit plants including watermelon and melon. a. citrulli strains can be divided into two major groups based on dna fingerprint analyses and biochemical properties. group i strains have been generally isolated from non-watermelon cucurbits, while group ii strains are closely associated with watermelon. in the present study, we report the genome sequence of m6, a group i model a. citrulli strain, isolated from me ...	2016	27092114
quorum-sensing contributes to virulence, twitching motility, seed attachment and biofilm formation in the wild type strain aac-5 of acidovorax citrulli.	acidovorax citrulli is a seed-borne pathogen causing bacterial fruit blotch of cucurbits including melon and watermelon. we investigated the roles of quorum sensing in the wild-type group ii strain aac-5 of a. citrulli by generating aacr and aaci knockout mutants and their complementation strains. we found that twitching motility and virulence were reduced, but biofilm formation and seed attachment were increased significantly in the two mutants as compared to the wild type strain. deletion of a ...	2016	27594669
selective detection of viable seed-borne acidovorax citrulli by real-time pcr with propidium monoazide.	in recent years, use of the dna-intercalating dye propidium monoazide (pma) in real-time pcr has been reported as a novel method to detect viable bacteria in different types of samples, such as food, environmental, and microbiological samples. in this study, viable cells of acidovorax citrulli, the causal agent of bacterial seedling blight and fruit blotch, were selectively detected and differentiated from dead cells by real-time fluorescent polymerase chain reaction amplification after the bact ...	2016	27739469
strains of the group i lineage of acidovorax citrulli, the causal agent of bacterial fruit blotch of cucurbitaceous crops, are predominant in brazil.	bacterial fruit blotch (bfb), caused by the seedborne bacterium acidovorax citrulli, is an economically important threat to cucurbitaceous crops worldwide. since the first report of bfb in brazil in 1990, outbreaks have occurred sporadically on watermelon and, more frequently, on melon, resulting in significant yield losses. at present, the genetic diversity and the population structure of a. citrulli strains in brazil remain unclear. a collection of 74 a. citrulli strains isolated from naturall ...	2016	27532426
self-paired monoclonal antibody lateral flow immunoassay strip for rapid detection of acidovorax avenae subsp. citrulli.	we screened a highly specific monoclonal antibody (mcab), named 6d, against acidovorax avenae subsp. citrulli (aac). single mcab 6d was used as both nanogold-labeled antibody and test antibody to develop a single self-paired colloidal gold immunochromatographic test strip (sa-gics). the detection limit achieved using the sa-gics approach was 10(5) cfu/ml, with a result that can be observed by the naked eye within 10 min. moreover, sa-gics can detect eight strains of aac and display no cross-reac ...	2016	27370686
tetranychus urticae (acari: tetranychidae) transmits acidovorax citrulli, causal agent of bacterial fruit blotch of watermelon.	the two-spotted spider mite (tssm) tetranychus urticae is one of the most important pests of cucurbit plants. if tssm can act as vector for acidovorax citrulli (acc), causal agent of bacterial fruit blotch (bfb), then the movement of mites from infected to healthy plants may represent a potential source of inocula for bfb outbreaks. to confirm the association between acc and tssm, we generated a green fluorescent protein-tagged mutant strain (acc02rf) by transposon mutagenesis and demonstrated t ...	2016	27178042
embryo localization enhances the survival of acidovorax citrulli in watermelon seeds.	acidovorax citrulli, the causal agent of bacterial fruit blotch (bfb) of cucurbits has been observed to survive for >34 years in stored melon and watermelon seeds. to better understand this remarkable longevity, we investigated the bacterium's tolerance to desiccation and the effect of bacterial localization in different watermelon seed tissues on its survival. we compared the ability of a. citrulli to tolerate desiccation on filter paper discs and on host (watermelon) and nonhost (cabbage, corn ...	2016	26756827
biological control of bacterial fruit blotch of watermelon pathogen (acidovorax citrulli) with rhizosphere associated bacteria.	bacterial fruit blotch (bfb), which is caused by acidovorax citrulli, is a serious threat to watermelon growers around the world. the present study was conducted to screen effective rhizobacterial isolates against 35 different a. citrulli isolates and determine their efficacy on bfb and growth parameters of watermelon. two rhizobacterial isolates viz. paenibacillus polymyxa (sn-22), sinomonas atrocyanea (nsb-27) showed high inhibitory activity in the preliminary screening and were further evalua ...	2017	28381964
structure of the cyanuric acid hydrolase trzd reveals product exit channel.	cyanuric acid hydrolases are of industrial importance because of their use in aquatic recreational facilities to remove cyanuric acid, a stabilizer for the chlorine. degradation of excess cyanuric acid is necessary to maintain chlorine disinfection in the waters. cyanuric acid hydrolase opens the cyanuric acid ring hydrolytically and subsequent decarboxylation produces carbon dioxide and biuret. in the present study, we report the x-ray structure of trzd, a cyanuric acid hydrolase from acidovora ...	2017	28345631
antagonistic activity and mode of action of phenazine-1-carboxylic acid, produced by marine bacterium pseudomonas aeruginosa pa31x, against vibrio anguillarum in vitro and in a zebrafish in vivo model.	phenazine and its derivatives are very important secondary metabolites produced from pseudomonas spp. and have exhibited broad-spectrum antifungal and antibacterial activities. however, till date, there are few reports about marine derived pseudomonas and its production of phenazine metabolites. in this study, we isolated a marine pseudomonas aeruginosa strain pa31x which produced natural product inhibiting the growth of vibrio anguillarum c312, one of the most serious bacterial pathogens in mar ...	2017	28289406
further characterization of genetically distinct groups of acidovorax citrulli strains.	bacterial fruit blotch of cucurbits (bfb) is caused by the gram-negative bacterium acidovorax citrulli, whose populations can be distinguished into two genetically distinct groups, i and ii. based on visual assessment of bfb severity on cucurbit seedlings and fruit after inoculation under greenhouse conditions, group i a. citrulli strains have been reported to be moderately to highly virulent on several cucurbit hosts, whereas group ii strains have exhibited high virulence on watermelon but low ...	2017	27618192