Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| equine herpesviruses: antigenic relationships and deoxyribonucleic acid densities. | equine herpesviruses with a deoxyribonucleic acid density of 1.716 to 1.717 g/cm(3) were compared with one another by the plaque-reduction test and by the rate of development of cytopathic effect as indicated by plaque size in rabbit kidney cultures. of the 19 isolates studied, the 9 which had already been tentatively labeled equine abortion viruses were serologically similar to one another; each of them grew more quickly than did any of the other 10 isolates although the mean plaque sizes forme ... | 1973 | 4742974 |
| [experimental genital and nasal infection of horses with the equine coital exanthema virus]. | 1974 | 4844323 | |
| coital exanthema in stallions. | equine coital exanthema can be produced experimentally in stallions by inoculation with an equine herpesvirus (strain 65/61) and be transmitted during coitus with an infected mare. serological responses to this infection include the production of complement-fixing and serum-neutralizing antibodies which reach maximum levels 14 to 21 days after infection. complement-fixing antibodies decline rapidly and are usually not detectable by 60 days after infection, whereas serum-neutralizing antibody act ... | 1975 | 173847 |
| cell-free synthesis of equine herpesvirus type 3 nucleocapsid polypeptides. | 1976 | 176794 | |
| equine herpesviruses. 6. sequential infection of horses with types 2, 3 and 1. | the immunological and virological status of 3 foals in respect of equine herpesviruses (ehv) was established and the foals were sequentially infected with ehv2, ehv3 and ehv1. following experimental infection with ehv2, no clinical signs of disease were observed in any foal. the inoculation of ehv3 into the genital tract resulted in lesions of the mucous membrane and perineal skin that were considered typical of equine coital exanthema. following intransal inoculation of ehv3 extensive ulceratio ... | 1976 | 183644 |
| equine herpesviruses: type 3 as an abortigenic agent. | the inoculation of equine herpesvirus type 3 (ehv3) strain 65/61 into the amniotic cavity of a mare 6-7 months pregnant resulted in abortion 11 days later. following abortion typical lesions of coital exanthema were not observed in the genital tract of the mare, nor was ehv3 isolated from her. serological evidence, however, indicated that the mare was infected with ehv3 following inoculation. grossly the foetal disease was characterised by placentitis, focal ulcerative dermatitis, focal necrosis ... | 1976 | 186003 |
| genetic relatedness of equine herpesvirus types 1 and 3. | the genetic relatedness of two types of equine herpesviruses (ehvs), 1 (ehv-1) and 3 (ehv-3), was determined by dna-dna reassociation kinetics. denatured, labeled viral dna probe was allowed to reassociate in the presence or absence of the second unlabeled viral dna. the initial rate of reassociation of either labeled viral dna was increased by the presence of the heterologous viral dna to an extent indicating only 2 to 5% homology between the two ehv genomes. moreover, labeled rna extracted fro ... | 1977 | 201775 |
| replication of equine herpesvirus type 3: kinetics of infectious particle formation and virus nucleic acid synthesis. | the kinetics of equine herpesvirus type 3 (ehv-3) multiplication and of the synthesis of ehv-3 specific dna and rna were investigated. a one-step growth curve of ehv-3 in equine epithelial cells from a transitional cell carcinoma was characterized by: (1) a short eclipse period (4 h); (2) an exponential increase in infectious virus between 5 and 10 h post-inoculation; and (3) a slow, inefficient release of newly formed virus into the extracellular fluid. two hours after infection of cells with e ... | 1977 | 558280 |
| replication of equine herpesvirus type 1 and type 3: resistance to hydroxyurea and thymidine. | the replication of equine herpesvirus type 1 (ehv-1) and type 3 (ehv-3) was unimpeded in three different cell types-equine epithelial cells, equine fibroblasts, and mouse fibroblasts-which had been blocked in their capacity to synthesize host dna by 2.5 mm hydroxyurea (hu) or 2 mm thymidine (tdr). the rate of dna synthesis in uninfected or equine herpesvirus-infected cells in the presence of hu or tdr was measured by pulse-labeling cell samples with a labeled dna precursor at different times aft ... | 1978 | 563855 |
| antigenic relatedness of equine herpes virus types 1 and 3. | antiserums prepared in specific pathogen free (spf) ponies were used in direct and indirect immunofluorescence, immunodiffusion, complement fixation and serum neutralization procedures to study the interrelationships of the three types of equine herpes viruses (ehv-1, ehv-2, and ehv-3). equine cell cultures infected with each type virus fluoresced when stained with homologous conjugated antiserum. in reciprocal tests ehv-1 and ehv-3 cross-fluoresced, but ehv-2 did not cross-fluoresce. non-infect ... | 1978 | 75724 |
| equine herpesvirus type 3 (equine coital exanthema) in new south wales. | 1979 | 232418 | |
| biochemical characterization of equine herpesvirus type 3-induced deoxythymidine kinase purified from lytically infected horse embryo dermal fibroblasts. | infection of horse kyed cells with equine herpesvirus type 3 (ehv-3) resulted in a sevenfold increase in cytosol deoxythymidine kinase (dtk) activity. the ehv-3 dtk was purified from kyed cytosol dtk by affinity chromatography on deoxythymidine-sepharose and characterized with respect to its electrophoretic mobility, molecular weight, substrate specificity, phosphate donor specificity, and immunological specificity. the purified ehv-3 dtk migrated in polyacrylamide gels with an rf of 0.30 and se ... | 1980 | 7373717 |
| herpesviral diseases affecting reproduction in the horse. | 1980 | 6262988 | |
| the effect of equine coital exanthema on the fertility of mares covered by stallions exhibiting the clinical disease. | the effect on the fertility of mares during two outbreaks of equine coital exanthema have been described. conception was not prevented, nor was pregnancy terminated in mares showing active lesions of coital exanthema. normal pregnancy rates occurred in mares served during active infection of the stallions. origin of the disease was not determined. | 1981 | 6266381 |
| properties of the genome of equine herpesvirus type 3. | 1982 | 6285597 | |
| analysis of small and large plaque variants of equine herpesvirus type 3 by restriction endonucleases. brief report. | six of 6 equine herpesvirus type 3 (ehv3) isolates, 5 of which were epidemiologically unrelated, produced a mixture of small and large plaque variants in equine foetal kidney cells under methylcellulose. in 4 of 4 instances the cleavage site(s) generating the bam hi a fragment of large plaque variants was distinct from the site(s) for the same fragment of small plaque variants. | 1983 | 6314939 |
| structure of the genome of equine herpesvirus type 3. | restriction endonuclease mapping studies were performed to determine the molecular structure of the genome of equine herpesvirus type 3 (ehv-3). purified ehv-3 dna, either unlabeled or 32p-labeled, was analyzed using the restriction enzymes bamhi, bcli, bglii, ecori, and hindiii. the findings that four 0.5 m (molar) fragments were present, that two of these were terminal fragments, and that all 0.5 m fragments contained homologous dna sequences as judged by dna hybridization analyses indicated t ... | 1984 | 6322418 |
| infectious skin diseases of horses. | 1984 | 6330957 | |
| molecular pathogenesis of equine coital exanthema: restriction endonuclease digestions of ehv-3 dna and indications of a unique xbai cleavage site. | equine herpesvirus type 3 (ehv-3) dna, isolated from purified virions of the large-plaque strain, was digested with the restriction endonucleases xbai, bg/ii, ecori, and hindiii. several lines of evidence indicated that the dna extracted from purified virions was composed of long (l) and short (s) components and was present as two isomeric forms, p and is. the evidence included: (i) after electrophoresis on agarose gels, the summed molecular weights of the digestion products exceeded that expect ... | 1985 | 2985521 |
| isolation of equine coital exanthema virus (equine herpesvirus 3) from the nostril of a foal. | the virus causing equine coital exanthema (equine herpesvirus 3) was isolated from a lesion on the nostril of a 2-month-old foal. one week after the mare had returned from a stallion station, vesicular lesions developed on her vulva. they were diagnosed clinically as coital exanthema, and 5 days later a lesion developed on the nostril of her foal. this case is an example of horse-to-horse transmission of coital exanthema virus without coitus. a laboratory diagnosis is necessary to differentiate ... | 1985 | 2997094 |
| molecular pathogenesis of equine coital exanthema: temperature-sensitive function(s) in cells infected with equine herpesviruses. | preliminary experiments have revealed that several laboratory and wild-type strains of the equine herpesvirus (ehv) triad were temperature-sensitive for growth when assayed at 39 degrees c. the efficiencies of plating (eop) observed were 10(-2) for both ehv 1 and 2, and 1 x 10(-6) for ehv 3. the eops were determined by plaque assays which compared titrations at 34 degrees c and 39 degrees c on equine fetal dermal fibroblast cells. growth yield experiments, assayed at 34 degrees c, reflected thos ... | 1986 | 3016974 |
| oncogenic transformation of primary hamster embryo cells by equine herpesvirus type 3. | infection of nonpermissive primary hamster embryo cells with equine herpesvirus type 3 (ehv-3; multiplicity of infection = 10 pfu/cell) resulted in an abortive infection and the development of several hundred foci of rapidly growing cells. five of these foci were chosen at random for the establishment of transformed cell lines, designated evd-1 (equine venereal disease) through 5. these transformed cell lines exhibited altered biological properties typical of transformed cells, including immorta ... | 1986 | 3765823 |
| molecular pathogenesis of equine coital exanthema (ece): temperature sensitivity (ts) and restriction endonuclease (re) fragment profiles of several field isolates. | examination of six field isolates of equine herpesvirus 3, the causative agent of equine coital exanthema, indicates that all were temperature sensitive (ts) at the body temperature, 39 degrees c, of their host (equine asinus and callabus) when grown in cell culture. the isolates were characterized by fingerprint analysis with the restriction endonucleases xbai, ecori, bamhi and hind iii to establish possible epidemiologic relatedness. three of the six isolates may be considered related. variati ... | 1987 | 3028334 |
| molecular pathogenesis of equine coital exanthema: identification and expression of infected cell polypeptides at the restricted temperature during equine herpesvirus 3 infection. | equine herpesvirus 3 (ehv-3)-infected equine cells display a kinetics of infected cell polypeptide (icp) synthesis at 34 degrees c that is typical of coordinate cascade gene regulation of herpesviruses. in contrast, when infected cell cultures are incubated at the restricted temperature of 39 degrees c, the shift from early (beta) gene expression to late (gamma) gene expression is perturbed, i.e., there is an accumulation of early (beta) gene products and a decrease in, or absence of, late (gamm ... | 1988 | 2852875 |
| equine coital exanthema (ehv-3 virus) infection in india. | a progenital disease encountered at one equine stud farm at bangalore in southern india during 1987 was investigated and confirmed as equine coital exanthema on the basis of characteristic lesions and clinical symptoms, isolation of equine herpes virus-3 (ehv-3) from the scabs collected from animals having active lesions and demonstration of neutralizing antibodies in the sera of recovered mares and stallion. this is the first authenticated report of the occurrence of equine coital exanthema in ... | 1989 | 2559563 |
| temperature sensitivity of equine herpesvirus isolates: a brief review. | this article reviews the findings on temperature sensitivity of equine herpesvirus isolates with an emphasis on equine herpesvirus 3, etiological agent of equine coital exanthema. the hypothesis is presented that the relative apathogenic nature of this herpesvirus may be an indirect result of its inability to synthesize and/or process glycoproteins needed by the virus to produce infectious virions at the normal body temperature of its natural host. it is suggested that equine herpesvirus 3 is th ... | 1990 | 1369251 |
| antiretroviral activity of synthetic hypericin and related analogs. | hypericin and pseudohypericin are naturally occurring polycyclic quinones which have recently been shown to inhibit the infectivity of several retroviruses, including human immunodeficiency virus. to better understand the antiviral mechanisms of these compounds, hypericin and a series of analogous quinones were synthesized and tested for anti-retroviral activity against equine infectious anemia virus (eiav). treatment of eiav-infected cells with hypericin reduced the production of infectious vir ... | 1990 | 1699534 |
| temporal regulation of equine herpesvirus type 3 transcription. | the transcription of equine herpesvirus type 3 (ehv-3; equine coital exanthema virus) has been examined and found to be temporally regulated into three classes: immediate early (ie), early (e), and late (l). hybridization of in vivo 32po4-labeled transcripts revealed that ie transcript(s) are derived exclusively from the inverted repeat segments (irs) of the viral genome, while e and l transcripts are not restricted to any specific region of the genome. northern blot analysis of ehv-3 ie rna rev ... | 1990 | 2157315 |
| venereal disease. | equine venereal infections of concern in the united states include ehv-3, t. equigenitalis, p. aeruginosa, and k. pneumoniae. stallions may also harbor eav in the genital tract and transmit the virus to mares during coitus. with the exception of ehv-3, the stallion generally remains asymptomatic while transmitting infections to mares during breeding. methods for diagnosis, treatment, and control of these infections are discussed. | 1992 | 1315616 |
| a type-specific conformational epitope on the nucleocapsid of equid herpesvirus-1 and its use in diagnosis. | a type-specific monoclonal antibody was produced by immunizing mice with purified equid herpesvirus-1 (ehv-1). the ehv-1 specific mab reacted with all the ehv-1 strains tested so far by indirect elisa, immunofluorescence, and immunoperoxidase tests. no reactions were detected with the ehv-4, ehv-2, or ehv-3 strains tested. the indirect immunofluorescence and immunoperoxidase tests showed that the nuclei of infected cells were predominantly stained by this mab. triton treatment of the virus and i ... | 1993 | 7688948 |
| antibodies against equine herpesviruses in free-ranging mountain zebras from namibia. | twenty-one blood samples of free-ranging mountain zebras (equus zebra) from namibia were tested for equine herpesvirus (ehv-1, -2, -3, -4) specific antibodies by immunofluorescence assay (ifa) and neutralization test (nt). additionally, type-specific nested polymerase chain reactions (nested pcr) were employed for detection of ehv-1, -2 and -4 dna. equine herpesvirus-1 antibodies were detected by ifa in all zebras, while only seven serum samples contained ehv-4 ifa antibodies. sera with high ifa ... | 1997 | 9391966 |
| study of the protective immunity of co-expressed glycoprotein h and l of equine herpesvirus-1 in a murine intranasal infection model. | equine herpesvirus-1 (ehv-1) glycoproteins h, and l (gh and gl) expressed individually or co-expressed by recombinant baculoviruses were used to immunise balb/c mice prior to intranasal challenge in a murine model of respiratory infection. only the co-expressed material (ehv-1 gh/gl) induced neutralising antibody (low levels). the same immunogen also produced the strongest cellular responses. immunisation with gh/gl and, to a lesser extent, with gh alone was associated with a reduction of virus ... | 1998 | 9595623 |
| detection of new dna polymerase genes of known and potentially novel herpesviruses by pcr with degenerate and deoxyinosine-substituted primers. | a consensus primer pcr approach was used to (i) investigate the presence of herpesviruses in wild and zoo equids (zebra, wild ass, tapir) and to (ii) study the genetic relationship of the herpesvirus of pigeons (columbid herpesvirus 1) to other herpesvirus species. the pcr assay, based on degenerate primers targeting highly conserved regions of the dna polymerase gene of herpesviruses, was modified by using a mixture of degenerate and deoxyinosine-substituted primers. the applicability of the mo ... | 1999 | 10456789 |
| the nucleotide sequence of the glycoprotein g homologue of equine herpesvirus 3 (ehv3) indicates ehv3 is a distinct equid alphaherpesvirus. | ehv3 causes equine coital exanthema and has been classified as an alphaherpesvirus on the basis of its biological properties; however due to the absence of any sequence information the phylogenetic relationship has not previously been examined. the complete nucleotide sequence of the ehv3 glycoprotein g (gg) gene was determined and showed that this virus is most closely related to the alphaherpesviruses equine herpesviruses type 1 (ehv 1) and type 4 (ehv4). ehv3 gg contains conserved and variabl ... | 1999 | 10550674 |
| identification of equine herpesvirus 3 (equine coital exanthema virus), equine gammaherpesviruses 2 and 5, equine adenoviruses 1 and 2, equine arteritis virus and equine rhinitis a virus by polymerase chain reaction. | to develop rapid (< 8 hour) tests using polymerase chain reaction (pcr) for the diagnosis of equine herpesvirus 3 (ehv3; equine coital exanthema virus), equine gammaherpesviruses 2 (ehv2) and ehv5, equine adenovirus 1 (eadv1), eadv2, equine arteritis virus (eav), equine rhinitis a virus (erav; formerly equine rhinovirus 1) | 2001 | 11712710 |
| the role of international transport of equine semen on disease transmission. | despite the numerous benefits of having the capability to transport semen internationally, there are serious potential ramifications if that semen is contaminated with a communicable disease. bacteria: many commensal bacteria colonize the exterior of the stallion penis and are not regarded as pathogenic. they may be cultured from an ejaculate. alterations of the normal bacterial flora on the exterior genitalia may cause the growth of opportunistic bacteria such as klebsiella pneumonia, pseudomon ... | 2001 | 11744267 |
| detection of equine herpesvirus 3 in equine skin lesions by polymerase chain reaction. | during a recent breeding season, ulcerative, pustular skin lesions were observed on the external genitalia of 2 mares and 1 stallion within a small herd. based on the location and description of the skin lesions plus the clinical history, equine coital exanthema, caused by equine herpesvirus 3 (ehv3), was the primary differential diagnosis. scrapings of skin lesions from the perineum of 2 mares were submitted for diagnostic evaluation. virus isolation was attempted by inoculation of several cell ... | 2004 | 14974851 |
| occurrence of equine coital exanthema in pastured draft horses and isolation of equine herpesvirus 3 from progenital lesions. | during the period from 2001 to the following year, progenital diseases had been epidemic among the draft stallions and mares pastured together in iwate prefecture, the northeastern district of japan. a stallion and 8 of 31 mares were affected in 2001, and 1 of 2 stallions and 10 of 36 mares in 2002. the clinical symptoms consisted of the formation of papules, pustules, ulcers and scabs on the progenital skin and mucosa in stallions and mares. in 2002, equine herpesvirus 3 (ehv3) was isolated fro ... | 2004 | 15644599 |
| temporal detection of equine herpesvirus infections of a cohort of mares and their foals. | the objectives of this study were to estimate the prevalence of equine herpesviruses (ehv) 1-5 in the nasal secretions (ns) of a cohort of 12 mares and their foals from birth to 6 months of age, estimate the prevalence of ehv-1-5 infection of peripheral blood mononuclear cells (pbmc) of selected foals, and investigate phylogenetic relationships amongst the various strains of ehv-2 and 5. virus-specific pcr assays were used to detect ehv-1-5 in ns and pbmc. a homologous portion of the glycoprotei ... | 2006 | 16774810 |
| establishment of a novel equine cell line for isolation and propagation of equine herpesviruses. | in the present study, an equine-derived cell line was established by transfecting primary fetal horse kidney (fhk) cells with expression plasmid encoding simian virus 40 (sv40) large t antigen and then cloning them by limiting dilution. the cloned cell line, named fhk-tcl3, grew well and could be propagated over 30 times by splitting them 1:3. equine herpesvirus (ehv)-1 and ehv-4 replicated well in fhk-tcl3. ehv-2 and ehv-4 were isolated from samples collected from horses in the field using fhk- ... | 2007 | 17917390 |
| herpesvirus-associated neurological disease in a donkey. | a 4-year-old donkey was evaluated for progressive neurological abnormalities consisting of depression, stupor, weakness, and recumbency. diagnostic evaluation for viral involvement identified an asinine herpesvirus in dna extracted from deep pharyngeal swabs. specific primers were designed based on comparison with equine herpesviral dna polymerase sequences and yielded an 875-base pair product from the donkey. this sequence had complete identity with short sequences of asinine herpesvirus previo ... | 2008 | 18987238 |
| experimental reactivation of equine herpesvirus-3 following corticosteroid treatment. | state of latency, well known for several herpesviruses, has been proposed for equine herpesvirus-3 (ehv-3) and supported by epidemiological observations. no detailed assessment about reactivation, patterns of excretion and reexcretion has been formally reported. an experimental reactivation study by corticosteroid treatment in previously naturally infected horses was therefore carried out. two polo mares with clinical and virologically confirmed history of equine coital exanthema were injected w ... | 2008 | 19031515 |
| antiviral effect of recombinant equine interferon-gamma on several equine viruses. | recombinant equine interferon-gamma (reifn-gamma) was prepared using a baculovirus expression system and its antiviral activity was investigated using several equine viruses. the reifn-gamma suppressed the replication of all equine viruses used in the present experiment in horse cell cultures, but did not affect the growth of host cells at concentrations of less than 1000 u/ml. a strong antiviral effect was observed, especially against rna viruses. equine picornavirus, equine rhinovirus and equi ... | 2010 | 20004981 |
| outbreak of rhinitis caused by equine herpesvirus type 3. | 2010 | 20139382 | |
| subclinical infection and periodic shedding of equid herpesvirus 3. | the temporary disruption of reproductive activities due to equine coital exanthema (ece), caused by equid herpesvirus 3 (ehv-3), at thoroughbred breeding facilities and embryo transfer centres, has an appreciable economic impact. the aim of the present study was to estimate the prevalence of excretion of ehv-3 in mares without clinical symptoms under field conditions and the re-excretion patterns of the virus in two seropositive (presumably latently infected) mares maintained in isolation for 11 ... | 2010 | 20494427 |
| equine coital exanthema and its potential economic implications for the equine industry. | equine coital exanthema (ece) caused by equid herpesvirus 3 (ehv-3) is a contagious venereal disease characterised by the formation of painful papules, vesicles, pustules and ulcers on the external genitalia of both mares and stallions. ehv-3 is an alphaherpesvirus that is distinct from the other equine herpesviruses and endemic in most horse breeding populations worldwide. the negative impacts of ece on equine breeding enterprises are the forced, temporary disruption of mating activities of mar ... | 2011 | 21354835 |
| serological survey of equine viral diseases in mongolia. | three hundred sera were collected from horses in various parts of mongolia in 2007 and seroepidemiological surveys for several equine viruses performed on them. equid herpesvirus 1 and equine rhinitis a virus were prevalent, and equine arteritis virus and equid herpesvirus 3 were detected over a wide area though their rates of antibody-positivity were not high. equine infectious anemia was distributed locally. the rates of horses antibody-positive for japanese encephalitis virus and equine influ ... | 2011 | 21447048 |
| experimental infection with equid herpesvirus 3 in seronegative and seropositive mares. | equine coital exanthema (ece), caused by equid herpesvirus 3 (ehv-3), has been recognized as an economically significant venereal disease for years. however, no infection models on the natural host have been established. in order to set up an experimental infection protocol, seronegative and seropositive mares were topically inoculated in the perineal region with 4 × 10(6)tcid(50)/ml of ehv-3. clinical signs were then evaluated by means of a designed scoring system, and body temperature was reco ... | 2012 | 22776511 |
| role of equine herpesviruses as co-infecting agents in cases of abortion, placental disease and neonatal foal mortality. | herpesviral infections frequently occur in horses. the objective of this study was to investigate the possible association of equine herpesviruses (ehv-1, ehv-2, ehv-3, ehv-4, ehv-5) with other causes of abortion, neonatal mortality or placental disorder. sixty-seven abortions, 22 stillbirths, 14 cases of neonatal foal mortality and 3 cases of placental disease were investigated for infectious and non-infectious causes. type-specific nested pcr assays and virus isolation were performed to detect ... | 2013 | 24052369 |
| complete genome sequence of equid herpesvirus 3. | equid herpesvirus 3 (ehv-3) is a member of the subfamily alphaherpesvirinae that causes equine coital exanthema. here, we report the first complete genome sequence of ehv-3. the 151,601-nt genome encodes 76 distinct genes like other equine alphaherpesviruses, but genetically, ehv-3 is significantly more divergent. | 2014 | 25278519 |
| replication characteristics of equine herpesvirus 1 and equine herpesvirus 3: comparative analysis using ex vivo tissue cultures. | replication kinetics and invasion characteristics of equine herpesvirus-1 and -3 (ehv-1/-3) in nasal and vaginal mucosae were compared using explants. the explants were cultured during 96 h with little change in viability. the tissues were inoculated with ehv-1 03p37 (neuropathogenic), 97p70 (abortigenic) and ehv-3 04p57, collected at 0, 24, 48 and 72 h post inoculation (pi) and stained for viral antigens. both ehv-1 and ehv-3 replicated in a plaquewise manner. the plaques were already observed ... | 2016 | 26768993 |
| isolation of equine herpesvirus 3 (ehv-3) from equine coital exanthema of two stallions and sero-epidemiology of ehv-3 infection in japan. | in the spring of 2015, two stallions reared in farms a and b in hokkaido in japan showed symptoms of equine coital exanthema. equine herpesvirus 3 (ehv-3) was isolated from penis swab samples of both stallions, and the isolates from each stallion in farms a and b were designated as ss-1 and ys-1 strains, respectively. bamhi restriction profiles of ss-1 and japanese reference strain iwate-1 were indistinguishable, but the bamhi-a fragment of ys-1 was larger than those of ss-1 and iwate-1 by 1.9 k ... | 2017 | 28132964 |
| occurrence of equine coital exanthema (ece) in stallions in japan and effectiveness of treatment with valacyclovir for ece. | equine coital exanthema (ece) has been reported in many countries, but equine herpesvirus 3 (ehv-3) has been isolated only once in japan. in 2015, symptoms of ece were found, and ehv-3 was isolated in two stallions. valacyclovir, an anti-herpesvirus agent, was administered orally. the stallions rested from mating for more than two weeks, causing enormous financial losses because of their high fees. this is the first study in which valacyclovir was administered for ece. though valacyclovir treatm ... | 2017 | 28123140 |
| construction and manipulation of a full-length infectious bacterial artificial chromosome clone of equine herpesvirus type 3 (ehv-3). | equine herpesvirus type 3 (ehv-3) is the causal agent of equine coital exanthema, a disease characterized by pox-like lesions on the penis of stallions and the vulva of mares. although the complete genomic sequence of ehv-3 has been recently made available, its genomic content remains poorly characterized and the molecular mechanisms of disease development not yet elucidated. in an attempt to facilitate genetic manipulation of ehv-3, we describe here the construction of a full-length infectious ... | 2017 | 27865864 |