Publications

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molecular characterization of cytoplasmic and nuclear genomes in phenotypically abnormal valencia orange (citrus sinensis) + meiwa kumquat (fortunella crassifolia) intergeneric somatic hybrids.organelle dna inheritance of four 10-year-old somatic hybrid trees between valencia orange [citrus sinensis (l.) osbeck] and meiwa kumquat (fortunella crassifolia swingle) was analyzed by cleaved amplified polymorphic sequence (caps) and restriction fragment length polymorphisms (rflps). five chloroplast (cp) and three mitochondrial (mt) universal primer pairs were amplified, but no polymorphisms were detected. when the polymerase chain reaction products were digested by 15 restriction enzymes, ...200312789447
[genetic variation of citrus calli revealed by the ploidy analyser].the cell dna content of forty-eight citrus calli of different genotype were measured by using the ploidy analyser. the results showed that 93.8% out of forty-eight citrus calli had double dna content except that ruby. weizhang and kinnow had little varied cells and did not show second peak. moreover, pineapple, meiwa kumquat, changsha, russ navel, guoqing no. 4 and carter had dna varied cells including triploids and aneuploids based on the dpac software analyse. among the forty-eight citrus call ...200312776606
production of intergeneric somatic hybrids between round kumquat (fortunella japonica swingle) and 'morita navel' orange (citrus sinensis osbeck).intergeneric somatic hybrids between embryogenic callus-derived protoplasts of round kumquat (fortunella japonica swingle) and 'morita navel' orange (citrus sinensis osbeck) were produced by electrofusion. among the eight different fusion strains obtained, six showed normal morphology, whereas the remaining two showed malformation. all the regenerated plants were intermediate in leaf morphology and had thick and round leaves, which are typical characteristics of polyploids. ploidy analyses by fl ...200414997338
isoflavonoids in the rutaceae family: 1. fortunella obovata, murraya paniculata and four citrus species.several types of compounds with immunoreactivity similar to isoflavonoids were detected in water: ethanol extracts of leaves of fortunella obovata hort. ex tanaka, murraya paniculata jack. and four citrus species, namely c. aurantium l, c. grandis osbeck, c. limonia osbeck., and c. sinensis osbeck (rutaceae). the chromatographic mobilities of the immunoreactive substances were compared with those of authentic standards, revealing a spectrum of isoflavonoid metabolites in all plants studied. agly ...200415508833
characteristic odor components of kumquat (fortunella japonica swingle) peel oil.this study was conducted to determine the composition of kumquat (fortunella japonica swingle) cold-pressed peel oil and to determine which volatile components are primarily responsible for the aroma of this oil. eighty-two compounds were identified in the oil by gc and gc-ms. the major compounds were limonene (93.73%), myrcene (1.84%), and ethyl acetate (1.13%). flavor dilution (fd) factors and relative flavor activities (rfa) of volatile constituents were evaluated by aroma extract dilution an ...200515740053
effects of nucleo-cytoplasmic interactions on leaf volatile compounds from citrus somatic diploid hybrids.three diploid citrus somatic hybrids (cybrids) were produced by fusions combining nucellar callus-derived protoplasts of willow leaf mandarin (citrus deliciosa ten.) and commune clementine (citrus clementina hort. ex tan.) with, respectively, leaf protoplasts of eureka lemon [citrus limon (l.) burm.] and marumi kumquat [fortunella japonica (thunb.) swing.] and leaf protoplasts of marumi kumquat. ploidy and origins of the nuclear, chloroplastic, and mitochondrial genomes were investigated by flow ...200515913319
influence of postharvest hot water treatment on nutritional and functional properties of kumquat (fortunella japonica lour. swingle cv. ovale) fruit.the present study investigated the influence of a hot water dip (hwd) for 2 min at 50 degrees c, a standard and effective treatment for postharvest decay control of citrus fruit, on the nutritional and health-related properties of kumquats. the results show that most of the parameters examined, including titratable acidity, soluble solids content, maturity index, glucose, fructose, sucrose, ascorbic acid, dehydroascorbic acid, alpha- and gamma-tocopherols, beta-carotene, zeaxantin, rhoifolin, an ...200818163539
the evolution of cma bands in citrus and related genera.most species of citrus and related genera display a similar karyotype with 2n = 18 and a variable number of terminal heterochromatic blocks positively stained with chromomycin a(3) (cma(+) bands). some of these blocks are 45s rdna sites, whereas others may correspond to the main gc-rich satellite dna found in several citrus species. in the present work, the distribution of the 45s rdna and the main satellite dna isolated from c. sinensis (cssat) were investigated by in situ hybridization in seve ...201020490650
inhibitory effects of fortunella japonica var. margarita and citrus sunki essential oils on nitric oxide production and skin pathogens.a number of essential oils from citrus peels are claimed to have biological activities. citrus peel, called 'jin-pi', is used in traditional medicine for digestion, severe cold, and fever. however, the antibacterial activities against skin pathogens and anti-inflammatory effects of the essential oils of citrus sunki (jingyul) and fortunella japonica var. margarita (gumgyul) have not yet been described. therefore, in this study, the essential oils of the citrus species c. sunki (cse) and f. japon ...201020350876
comprehensive analysis of ccch-type zinc finger gene family in citrus (clementine mandarin) by genome-wide characterization.the ccch-type zinc finger proteins comprise a large gene family of regulatory proteins and are widely distributed in eukaryotic organisms. the ccch proteins have been implicated in multiple biological processes and environmental responses in plants. little information is available, however, about ccch genes in plants, especially in woody plants such as citrus. the release of the whole-genome sequence of citrus allowed us to perform a genome-wide analysis of ccch genes and to compare the identifi ...201424820208
isolation and characterization of ten microsatellite loci for wild citrus japonica (rutaceae). 201425201823
analysis of biologically active oxyprenylated ferulic acid derivatives in citrus fruits.4'-geranyloxyferulic (gofa) and boropinic acid have been discovered during the last decade as interesting phytochemicals having valuable pharmacological effects as cancer chemopreventive, anti-inflammatory, neuroprotective, and anti-helicobacter pylori agents. a reverse-phase hplc-uv/vis method for the separation and quantification of the title oxyprenylated ferulic acid derivatives in extracts obtained from peels of nine edible citrus and fortunella fruits was successfully applied. concentratio ...201424928688
drying effect on flavonoid composition and antioxidant activity of immature kumquat.a seven flavonoids in hot water extract of immature kumquat (citrus japonica var. margarita) were identified and quantified (mg/100g fresh fruit): 3',5'-di-c-β-glucopyranosylphloretin (dgpp, 285.9 ± 2.9 mg/100g), acacetin 8-c-neohesperidoside (margaritene, 136.2 ± 2.6 mg/100g), acacetin 6-c-neohesperidoside (isomargaritene, 119.1 ± 1.8 mg/100g), fortunellin (acacetin 7-o-neohesperidoside, 28.5 ± 0.7 mg/100g), apigenin 8-c-neohesperidoside (16.9 ± 0.1mg/100g), poncirin (isosakuranetin 7-o-neohesp ...201525308680
chemical constituents and antioxidant activity of essential oil and organic extract from the peel and kernel parts of citrus japonica thunb. (kumquat) from iran.the constituents of essential oils and organic extracts from peel and kernels of citrus japonica were analysed by gc and gc/ms. the content of essential oil in peel and kernel was 1.1 and 0.8% based on dry weight. the essential oil of c. japonica peel and kernel was characterised by a higher amount of limonene (51.0 and 47.1%) and germacrene d (12.1 and 6.3%), and the hexane extracts of its peel and kernel were characterised by a higher amount of dodecanol-1(12.9 and 20.8%) and linolenic acid (1 ...201626500054
chemical composition of the fruit oils of five fortunella species grown in the same pedoclimatic conditions in corsica (france).fruit oil from five species of kumquat (fortunella japonica, f. margarita, f. crassifolia, f. obovata, and f. hindsii) grown in the same pedoclimatic conditions have been analyzed by a combination of chromatographic and spectroscopic techniques. the compositions of the five fruit oils were strongly dominated by limonene (84.2-96.3%). other components present with appreciable contents were myrcene (1.3-12.9%) and germacrene d (0.3-2.4%).201627032215
chemical profiling using uplc q-tof/ms and antioxidant activities of fortunella fruits.the fruits of fortunella swingle are widely consumed as fresh fruits and traditional medicine in china. china is the origin center and has the largest cultivated area of the genus fortunella. in this study, the chemical compositions of ethanol extracts of the major fortunella cultivated types including fortunella japonica swingle, fortunella margarita swingle, fortunella crassifolia swingle 1 (lanshang) and fortunella crassifolia swingle 2 (liuyang) were determined using ultra performance liquid ...201627243926
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