Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| human infections due to malassezia spp. | the genus malassezia contains three member species: malassezia furfur and malassezia sympodialis, both obligatory lipophilic, skin flora yeasts of humans, and malassezia pachydermatis, a nonobligatory lipophilic, skin flora yeast of other warm-blooded animals. several characteristics suggest the basidiomycetous nature of these yeasts, although a perfect stage has not been identified. classically, these organisms are associated with superficial infections of the skin and associated structures, in ... | 1992 | 1576583 |
| isolation of malassezia sympodialis from feline skin. | carriage of malassezia yeasts was investigated in 17 cats in two colonies using a lipid-supplemented culture medium. malassezia pachydermatis was isolated from one cat. lipid-dependent malassezia yeasts with electrophoretic karyotypes consistent with m. sympodialis were isolated from all six cats in one group and from one of 11 in the second group. to our knowledge, this is the first report of the isolation of lipid-dependent yeasts from cats. | 1996 | 8732361 |
| isolation of malassezia sympodialis and malassezia globosa from healthy pet cats. | 1997 | 9292976 | |
| [taxonomy of malassezia furfur: state of the art]. | malassezia furfur is a lipophilic yeast considered as a normal component of the human skin flora. apart from pityriasis versicolor, m. furfur has been linked to several skin diseases such as seborrheic dermatitis, folliculitis or atopic dermatitis. moreover, these yeasts have been reported as agent of invasive human diseases including pneumonia, catheter-associated sepsis and peritonitis. the existence of morphological, serological, metabolical, biochemical and karyotipical differences has been ... | 1997 | 15538815 |
| is common neonatal cephalic pustulosis (neonatal acne) triggered by malassezia sympodialis? | a type of neonatal cephalic pustulosis that is clinically similar to classic neonatal acne recently has been linked to malassezia furfur infection. to correlate the mycological and clinical findings in neonates with cephalic pustulosis, we carried out a prospective case-control study in a neonatal unit from february to april 1997 using new techniques for classifying malassezia species. | 1998 | 9722730 |
| different utilization of neutral lipids by malassezia furfur and malassezia sympodialis. | in recent years, the genus malassezia has been expanded based on molecular data; in addition to m. furfur and m. pachydermatis, five new species (m. sympodialis, m. globosa, m. obtusa, m. restricta, m. slooffiae) have been described. apart from their lipid dependence, little is known about the metabolism and nutritional requirements of these new species. defined inocula of malassezia reference strains were cultured on selective agar for pathogenic fungi which was overlaid with olive oil. samples ... | 1998 | 9776806 |
| characterization of malassezia species by means of phenotypic characteristics and detection of electrophoretic karyotypes by pulsed-field gel electrophoresis (pfge). | a total of 220 isolates of the genus malassezia obtained from affected skin of animals and humans were included in this study. the isolates were examined for their belonging to the known species malassezia pachydermatis, malassezia sympodialis, malassezia furfur, malassezia obtusa, malassezia restricta, malassezia globosa and malassezia slooffiae by means of phenotypic and biochemical characteristics as well as their ability to assimilate polyethylen(20)-sorbitan-esters (tween 20, 40, 60, 80). t ... | 1999 | 10536433 |
| [isolation and identification of malassezia spp. in pytiriasis versicolor, seborrheic dermatitis and healthy skin.]. | the taxonomy of the genus malassezia has been recently revised and enlarged to include seven distinct species. the aim of the study was to analyse the prevalence of these species in the cutaneous lesions of pityriasis versicolor and dermatitis seborrhoeica, as well as in the normal skin. seventy-five patients with pityriasis versicolor, 75 of dermatitis seborrhoeica, and 150 samples of normal skin (75 from the forehead and 75 from the shoulders) were studied. a direct microscopy with koh + parke ... | 1999 | 18473598 |
| malignant otitis externa caused by malassezia sympodialis. | malignant otitis externa caused by fungal infections is rare. a review of the literature showed only 9 cases, and the causative fungus in all cases was aspergillus. this article reports an unusual case caused by malassezia sympodialis. | 2000 | 10585610 |
| otitis externa associated with malassezia sympodialis in two cats. | the lipid-dependent species malassezia sympodialis was isolated from two cats with otitis externa. to our knowledge, this is the first report of the isolation of lipid-dependent species of the genus malassezia associated with skin disease in domestic animals. | 2000 | 10699037 |
| the effects of lipid extraction on the immunomodulatory activity of malassezia species in vitro. | malassezia spp. are believed to be the causative agents of pityriasis versicolor and are strongly implicated in seborrhoeic dermatitis. the yeast also forms part of the normal human cutaneous microflora. we have previously shown that when malassezia yeast cells are incubated with human peripheral blood mononuclear cells (pbmcs), they are capable of reducing the levels of pro-inflammatory cytokines produced. in order to test the hypothesis that this immunoevasive phenomenon may be related to the ... | 2000 | 10892993 |
| adherence of malassezia pachydermatis and malassezia sympodialis to canine, feline and human corneocytes in vitro. | 2000 | 11079443 | |
| [the yeast malassezia on the skin of healthy individuals and patients with atopic dermatitis]. | the lipophilic yeast malassezia spp. from the skin of 32 healthy individuals and 21 patients with atopic dermatitis was isolated and identified. malassezia sympodialis was shown to be most common in both groups, m. globosa was less frequently encountered. moreover, no malassezia was found in 34 and 53% of cases, respectively. there was a relationship of the skin colonization of malassezia to the patients' age. malassezia spp. cultures whose appurtenance was impossible to identified by the guillo ... | 2001 | 11338502 |
| epidemiology of malassezia yeasts associated with pityriasis versicolor in ontario, canada. | the genus malassezia was recently revised to include seven species, but the clinical significance of each of these species is not clearly understood. to obtain a better understanding of the contribution of individual malassezia species to the epidemiology of pityriasis (tinea) versicolor, we used leeming-notman medium to culture patient skin specimens showing positive evidence of malassezia infection in direct microscopy. isolates were identified on the basis of recently published morphological ... | 2001 | 11346269 |
| quantitative culture of malassezia species from different body sites of individuals with or without dermatoses. | quantitative cultures were obtained using contact plates to determine whether the quantity and composition of malassezia species at a given anatomic site in normal individuals differs from that of patients with various cutaneous dermatoses. the sample included 20 clinically healthy individuals (without any dermatosis) and 110 patients with dermatoses (including 31 with atopic dermatitis [ad], 28 with psoriasis [ps], 28 with seborrheic dermatitis [sd] and 23 with pityriasis versicolor [pv]). cont ... | 2001 | 11446527 |
| [a case of central venous catheter-related infection with malassezia sympodialis]. | we report a 63-year-old male with central venous catheter-related infection caused by malassezia sympodialis after total gastrectomy for a gastric cancer. he had fever and his leukocyte counts and c-reactive protein were elevated 14 days after his operation. after his central venous hyperalimentation catheter was removed, the inflammatory signs immediately disappeared, suggesting an intravenous catheter-related infection. a yeast-like fungus was cultured in brain-heart infection semi-solid agar ... | 2001 | 11704751 |
| skin colonization by malassezia species in neonates: a prospective study and relationship with neonatal cephalic pustulosis. | to assess skin colonization by malassezia species in full-term healthy newborns, to investigate factors associated with colonization, and to look at acnelike cephalic pustulosis associated with this carriage. | 2002 | 11843642 |
| pityriasis versicolor rubra. | we report six typical cases with pityriasis versicolor (pv) rubra, with a background of collagen diseases in five cases and none in one case. two cases of systemic lupus erythematosus (sle) and one case of systemic scleroderma (ssc) had both pv rubra and nigra on the trunk. diagnosis of superficial infections of pv was made by microscopic examination of skin scrapings following koh, and many small whitish colonies were obtained in sabouraud's slant agar medium culture containing cycloheximide wi ... | 2002 | 11872414 |
| malassezia species in skin diseases. | since the taxonomic revision carried out in 1996, enlarging the genus malassezia to comprise seven different species, a number of studies have investigated from different points of view -- mycological, molecular and immunological -- the relationships of these species with the pathologies associated with lipophilic yeasts, as well as its presence in healthy skin. from these studies, it now appears clear that malassezia globosa is the main species associated with pityriasis versicolor, which is th ... | 2002 | 11964913 |
| identification and distribution of a novel malassezia species yeast on normal equine skin. | this study aimed to investigate the distribution of malassezia species yeasts on the skin of healthy horses. acetate tape samples were obtained from the lip, axilla, interbulbar region, groin and anus of 12 healthy horses. the samples were stained and examined microscopically and sites harbouring yeast-like organisms were identified. contact plates were applied to the skin at these sites and cultured at 26 degrees c and 32 degrees c. no growth was obtained on horse blood, sabouraud's dextrose or ... | 2002 | 11999275 |
| differentiation of malassezia furfur and malassezia sympodialis by glycine utilization. | the genus malassezia has been revised to include six lipophilic species and one nonlipophilic species. these malassezia species have been investigated to differentiate their morphological and physiological characteristics. however, assimilation of amino acids as a nitrogen source by these species was not well elucidated. in the present study, isolates of malassezia species were examined with a glycine medium (containing 7-266 mmol glycine, 7.4 mmol kh(2)po(4), 4.1 mmol mgso(4)7h(2)o, 29.6 mmol t ... | 2002 | 12100536 |
| modified method for evaluation of plasma membrane integrity in eukaryotic cell. | we propose a method for evaluation of the number of viable cells by the content of bromcresol purple dye absorbed by dead cells from the incubation medium. myramistin was used as a pore-forming agent. the number of live cells in yeast suspension inversely correlated with the percentage of dye absorbed by cells. the method is simple and requires no special equipment. the effect of myramistin on candida albicans and malassezia sympodialis cells and on epitheliocytes was evaluated. two-hour treatme ... | 2002 | 12459884 |
| detection and mutation screening of malassezia sympodialis sequences coding for the mal s 1 allergen implicated in atopic dermatitis. | 2003 | 14675212 | |
| detection of mala f and mala s allergen sequences within the genus malassezia. | malassezia species are opportunistic yeasts that are involved in the pathogenesis of a number of skin diseases including atopic eczema/dermatitis syndrome. previously, we cloned six allergens from malassezia sympodialis isolate atcc 42132; these allergens are designated mala s 1, and mala s 5-mala s 9. three additional allergens, mala f 2-mala f 4, have been isolated from m. furfur by other investigators. the objective of the present study was to investigate the presence of these mala sequences ... | 2003 | 14725321 |
| malassezia nana sp. nov., a novel lipid-dependent yeast species isolated from animals. | five isolates of a novel species of the yeast genus malassezia were isolated from animals in japan and brazil. phylogenetic trees based on the d1/d2 domains of the large-subunit (26s) rdna sequences and nucleotide sequences of the internal transcribed spacer 1 region showed that the isolates were conspecific and belonged to the genus malassezia. they were related closely to malassezia dermatis and malassezia sympodialis, but were clearly distinct from these two species and the other six species ... | 2004 | 15023986 |
| cloning, expression and characterization of two new ige-binding proteins from the yeast malassezia sympodialis with sequence similarities to heat shock proteins and manganese superoxide dismutase. | malassezia sympodialis is an opportunistic yeast that colonizes human skin and may induce ige and t cell reactivity in patients with atopic eczema/dermatitis syndrome (aeds). previously, we have cloned and expressed six recombinant allergens (rmala s 1 and rmala s 5 to rmala s 9) from this yeast. by combining high throughput screening and phage surface display techniques, 27 complete and partial ige-binding clones of m. sympodialis have been identified. here we enlarged the panel of recombinant ... | 2004 | 15128298 |
| no apparent association between periocular and ocular microcolonization and the degree of inflammation in patients with atopic keratoconjunctivitis. | the cause of the chronic inflammation in atopic keratoconjunctivitis (akc), the ocular manifestation of atopic eczema/dermatitis syndrome, is largely unknown. | 2004 | 15144463 |
| occurrence of malassezia species in healthy and dermatologically diseased dogs. | the presence of malassezia spp. yeasts was investigated in dermatological specimens of 224 dogs, 164 dermatologically diseased and 60 normal dogs. subjects included in the study were of different breed, age, sex and habitat. malassezia spp. positive cultures were obtained in 142 (63.4%) specimens: 67.6% from dermatologically diseased subjects and 51.6% from healthy dogs. malassezia pachydermatis, either as a pure culture or in association with lipid-dependent species, was identified in 138 (97%) ... | 2004 | 15281400 |
| malassezia sympodialis stimulation differently affects gene expression in dendritic cells from atopic dermatitis patients and healthy individuals. | it is known that 28-84% of patients with atopic dermatitis exhibit ige and/or t-cell reactivity to the opportunistic yeast malassezia sympodialis, which can be taken up by immature monocyte-derived dendritic cells (mddcs), resulting in mddc maturation. the aim of this study was to investigate whether mddcs from patients with atopic dermatitis respond differently to m. sympodialis compared to mddcs from healthy individuals. immature mddcs were stimulated with m. sympodialis and the gene expressio ... | 2004 | 15370698 |
| superficial fungal infections. | superficial fungal infections arise from a pathogen that is restricted to the stratum corneum, with little or no tissue reaction. in this seminar, three types of infection will be covered: tinea versicolor, piedra, and tinea nigra. tinea versicolor is common worldwide and is caused by malassezia spp, which are human saprophytes that sometimes switch from yeast to pathogenic mycelial form. malassezia furfur, malassezia globosa, and malassezia sympodialis are most closely linked to tinea versicolo ... | 2004 | 15451228 |
| molecular analysis of malassezia sympodialis-related strains from domestic animals. | recently, several new lipid-dependent species belonging to the genus malassezia have been described. some of them, such as malassezia dermatis, malassezia nana, and the tentatively named "malassezia equi," have similar phenotypes and are genetically close to malassezia sympodialis simmons et gueho 1990. dna characterization by d1/d2 26s rrna gene and internal transcribed spacer (its)-5.8s rrna gene sequencing analysis of lipid-dependent strains from different animal species close to m. sympodial ... | 2005 | 15634983 |
| prevalence of malassezia spp. in healthy pigs. | in the present study, we evaluated the occurrence of malassezia spp. from superficial areas (ear, anus, forehead and back) in 100 healthy pigs. all cultures from anus, forehead and back were negative for malassezia spp. a total of 73% of pigs were colonized by malassezia yeasts in the external ear canals. we isolated only lipid-dependent species (malassezia sympodialis 58%, malassezia slooffiae 30%). this study evaluates the occurrence of malassezia spp. in pigs since the taxonomic revision in 1 ... | 2005 | 15679660 |
| mycological examinations on the fungal flora of the chicken comb. | a total of 500 combs of adult chickens from two different locations in germany (hessen and schleswig-holstein) were clinically and mycologically examined. the chickens came from three battery cages (n = 79), one voliere system (n=32), six flocks maintained on deep litter (n = 69) and 12 flocks kept on free outdoor range (n=320). twenty-two of the 500 chicken combs (4.4%) were found to have clinical signs: only non-specific lesions neither typical of mycosis nor of avian pox such as desquamation ... | 2005 | 15743428 |
| ige-mediated and t cell-mediated autoimmunity against manganese superoxide dismutase in atopic dermatitis. | autoreactivity of patients with atopic dermatitis (ad) to human proteins has been postulated as a decisive pathogenetic factor for ad. | 2005 | 15867868 |
| isolation of malassezia species from healthy cats and cats with otitis. | lipid-dependent malassezia species have recently been cultured from veterinary specimens. the identification of malassezia species isolates from animals is important to clarify the epidemiology of these lipophilic yeasts. malassezia species were cultured from the external ear canals of 63 out of 99 cats with otitis and 12 of 52 (23%) healthy control cats. the rate of isolation in affected animals versus controls was highly significant (p<0.01). malassezia pachydermatis was isolated as a pure cul ... | 2005 | 15922220 |
| manganese superoxide dismutase in pathogenic fungi: an issue with pathophysiological and phylogenetic involvements. | manganese-containing superoxide dismutases (mnsods) are ubiquitous metalloenzymes involved in cell defence against endogenous and exogenous reactive oxygen species. in fungi, using this essential enzyme for phylogenetic analysis of pneumocystis and ganoderma genera, and of species selected among ascomycota, basidiomycota and zygomycota, provided interesting results in taxonomy and evolution. the role of mitochondrial and cytosolic mnsods was explored in some pathogenic basidiomycota yeasts (cryp ... | 2005 | 16055318 |
| malassezia and candida colonisation on glans penis of circumcised men. | the malassezia yeast are members of the normal human cutaneous flora in adults. they also are reported as part of the microflora of the male genital region in mostly uncircumcised males. it has been reported that malassezia sympodialis and malassezia globosa are the most frequent yeast belonging to the resident microflora of the penis as in other human skin areas. the aim was to evaluate the prevalence of malassezia and candida yeast colonisation on the glans penis of circumcised males. impressi ... | 2005 | 16115108 |
| the role of sensitization to malassezia sympodialis in atopic eczema. | atopic eczema (ae) is a chronic relapsing, highly pruritic inflammation of the skin with a worldwide prevalence of 10-20% in children and of 1-3% in adults. malassezia sympodialis has been reported as the most frequent skin-colonizing yeast in both ae patients and healthy individuals. approximately 50% of the ae patients show immediate-type skin reactions or have specific serum ige against m. sympodialis. sensitization to the yeast occurs almost exclusively in ae patients. the main cause for thi ... | 2006 | 16354952 |
| etiology of fungaemia and catheter colonisation in argentinean paediatric patients. | yeast strains obtained from blood cultures and catheters from intensive care units (icu) and hospitalised oncology paediatrics were studied. yeast were the first cause of catheter colonisation (51/627), and the third cause of bloodstream infection (44/6065). in catheter, the most frequent species were candida albicans (34%), c. parapsilosis (27.7%) and c. tropicalis (15%). in blood, c. albicans (40.8%), c. parapsilosis (26.6%), c. tropicalis (15%). malassezia furfur and malassezia sympodialis we ... | 2006 | 16367819 |
| analysis of the cross-reactivity and of the 1.5 a crystal structure of the malassezia sympodialis mala s 6 allergen, a member of the cyclophilin pan-allergen family. | cyclophilins constitute a family of proteins involved in many essential cellular functions. they have also been identified as a panallergen family able to elicit ige-mediated hypersensitivity reactions. moreover, it has been shown that human cyclophilins are recognized by serum ige from patients sensitized to environmental cyclophilins. ige-mediated autoreactivity to self-antigens that have similarity to environmental allergens is often observed in atopic disorders. therefore comparison of the c ... | 2006 | 16483252 |
| cutaneous hypersensitivity to malassezia sympodialis and dust mite in adult atopic dermatitis with a textile pattern. | atopic dermatitis (ad) patients with predominantly head and neck involvement react to patch tests of the yeast malassezia sympodialis (ms). protein patch testing methods and interpretation are controversial, but subgroups of ad patients may have unique triggers for disease activity. the aim of the study was to identify clinical characteristics of patients who are patch test-positive to dermatophagoides farinae/pteronyssinus (df) and ms and characterize cutaneous cytokine profiles of the atopy pa ... | 2006 | 16487281 |
| crystallization and preliminary crystallographic study of the yeast malassezia sympodialis allergen mala s 1. | the opportunistic yeast malassezia sympodialis can act as an allergen and elicit specific ige- and t-cell reactivity in patients with atopic eczema. the first identified major allergen from m. sympodialis, mala s 1, is present on the cell surface of the yeast. recombinant mala s 1 was expressed in escherichia coli, purified and refolded in a soluble form. crystals of mala s 1 were obtained in 25% peg 8k, 0.2 m (nh4)2so4. crystals belong to space group p2(1)2(1)2, with unit-cell parameters a = 44 ... | 2006 | 16511273 |
| malassezia sympodialis differently affects the expression of ll-37 in dendritic cells from atopic eczema patients and healthy individuals. | atopic eczema (ae) is a multifactorial disease, which has increased in prevalence. the skin-colonizing yeast malasezzia sympodialis can induce ige- and t-cell reactivity in patients with ae. ll-37 is an endogenous peptide antibiotic belonging to the cathelicidin family. the aim of this study was to examine whether exposure to m. sympodialis would affect the expression of ll-37 in dendritic cells. | 2006 | 16512803 |
| treatment of head and neck dermatitis with ciclopiroxolamine cream--results of a double-blind, placebo-controlled study. | in atopic dermatitis, microbial allergens may be pathogenetically significant. apart from staphylococcus aureus, these are primarily lipophilic malassezia yeasts. they are particularly involved in the pathogenesis of head and neck dermatitis (hnd), a special form of atopic dermatitis, which is often difficult to treat. fifty patients (21 men, 29 women) with moderate to severe hnd of at least 6 months' duration were included in a prospective double-blind study. all of them showed at least 10% inv ... | 2006 | 16612143 |
| susceptibility of yeast isolates from cattle with otitis to aqueous solution of povidone iodine and to alcohol-ether solution. | lipid-dependent malassezia species, candida spp. and rhodotorula mucilaginosa have been associated with bovine parasitic otitis. this paper evaluated the susceptibility of 63 yeast isolates from cattle with otitis to a povidone iodine aqueous solution (1% and 0.5% v/v) and to an alcohol-ether solution (1:1 v/v). the effectiveness of these antiseptics was assessed using the european suspension test. products achieving equal to or greater than 5-log reduction in numbers of the challenge organism a ... | 2006 | 16772232 |
| isolation of malassezia spp. from cerumen of wild felids. | the objective of this study was to determine the presence of different species of the genus malassezia in the healthy external auditory canal of wild felids maintained in captivity. one hundred and thirty-two adult animals (264 samples of cerumen), 77 males (58.3%) and 55 females (41.7%), were studied: large felids (55 animals) - 26 lions (panthera leo), 13 tigers (panthera tigris), 6 leopards (panthera pardus), 6 jaguars (panthera onca), 2 cheetahs (acinonyx jubatus), 2 pumas (puma concolor); s ... | 2006 | 16772235 |
| sensitization to the yeast malassezia sympodialis is specific for extrinsic and intrinsic atopic eczema. | the opportunistic yeast malassezia sympodialis belongs to the normal cutaneous flora but can also cause ige-mediated sensitization in patients suffering from atopic eczema (ae). we investigated 706 individuals by immunocapm70 and skin-prick tests with a crude m. sympodialis extract. in ae patients, we further performed skin prick tests, atopy patch tests, elisa, and peripheral blood mononuclear cells proliferation assays with recombinant m. sympodialis allergens (rmala s 1 and 5-9). in 52/97 pat ... | 2006 | 16778796 |
| higher ph level, corresponding to that on the skin of patients with atopic eczema, stimulates the release of malassezia sympodialis allergens. | the opportunistic yeast malassezia is a trigger factor in atopic eczema (ae). around 30-80% of patients with ae have an ige and/or t-cell reactivity to the yeast. several ige-binding components have been identified in malassezia extracts and 11 allergens have been cloned and sequenced. the ph of the skin surface in patients with ae is higher than that of normal healthy skin. we here investigate whether different ph conditions mimicking those of ae skin and healthy skin can influence the producti ... | 2006 | 16867055 |
| erlotinib associated with rosacea-like folliculitis and malassezia sympodialis. | 2006 | 16882195 | |
| [identification of malassezia species in patients with pityriasis versicolor in goiânia-go]. | the objectives of this study were to investigate the frequency of pityriasis versicolor occurrence and to identify yeasts of the genus malassezia in patients at the mycology laboratory of the federal university of goiás, in goiânia, state of goiás. ninety-five cases of pityriasis versicolor were diagnosed, and four species of malassezia were identified: malassezia furfur, malassezia sympodialis, malassezia globosa and malassezia obtusa. | 2006 | 17308710 |
| mala s 12 is a major allergen in patients with atopic eczema and has sequence similarities to the gmc oxidoreductase family. | atopic eczema (ae) is a chronic inflammatory skin disorder, characterized by impaired skin barrier and itch. the yeast malassezia belongs to the normal human skin microflora and can induce ige- and t-cell-mediated allergic reactions in ae patients. previously, we have identified several ige-binding components in malassezia sympodialis extract. | 2007 | 17313403 |
| two new lipid-dependent malassezia species from domestic animals. | during a study on the occurrence of lipid-dependent malassezia spp. in domestic animals, some atypical strains, phylogenetically related to malassezia sympodialis simmons et guého, were shown to represent novel species. in this study, we describe two new taxa, malassezia caprae sp. nov. (type strain ma383=cbs 10434), isolated mainly from goats, and malassezia equina sp. nov. (type strain ma146=cbs 9969), isolated mainly from horses, including their morphological and physiological characteristics ... | 2007 | 17367513 |
| crystal structure of the major malassezia sympodialis allergen mala s 1 reveals a beta-propeller fold: a novel fold among allergens. | atopic eczema (ae) is a chronic inflammatory disease in which genetic predisposition and environmental factors such as microorganisms contribute to the symptoms. the yeast malassezia sympodialis, part of the normal human cutaneous flora, can act as an allergen eliciting specific ige and t-cell reactivity in patients with ae. the major m. sympodialis allergen mala s 1 is localized mainly in the yeast cell wall and exposed on the cell surface. interestingly, mala s 1 does not exhibit any significa ... | 2007 | 17481656 |
| [concordance between phenotypical features and pcr-rea for the identification of malassezia spp]. | the genus malassezia has been recently revised and nowadays includes 11 species that cannot always be differentiated from each other by physiological and morphological tests. this study was aimed to evaluate the correlation between a molecular method and conventional phenotypic features in the identification of malassezia spp. to achieve this aim, 92 argentinean clinical strains isolated between 2001 and 2005 were analyzed along with three reference strains (malassezia furfur cbs 7019, malassezi ... | 2007 | 18095760 |
| cross-reactivity and 1.4-a crystal structure of malassezia sympodialis thioredoxin (mala s 13), a member of a new pan-allergen family. | we have identified thioredoxins (trx) of malassezia sympodialis, a yeast involved in the pathogenesis of atopic eczema, and of aspergillus fumigatus, a fungus involved in pulmonary complications, as novel ige-binding proteins. we show that these trx, including the human enzyme, represent cross-reactive structures recognized by serum ige from individuals sensitized to m. sympodialis trx. moreover, all three proteins were able to elicit immediate-type allergic skin reactions in sensitized individu ... | 2007 | 17182577 |
| the malassezia sympodialis allergen mala s 11 induces human dendritic cell maturation, in contrast to its human homologue manganese superoxide dismutase. | recently, we identified a major malassezia sympodialis allergen, mala s 11, which displays a high degree of dna sequence homology to human manganese superoxide dismutase (hmnsod). in atopic eczema patients sensitized to m. sympodialis, hmnsod can elicit eczematous reactions and positive skin prick tests, suggesting cross- reactivity to mala s 11 based on molecular mimicry. the objective of the current study was to compare the influence of mala s 11 and hmnsod on human dendritic antigen-presentin ... | 2007 | 17259733 |
| allergen motifs and the prediction of allergenicity. | we have recently shown that the majority of allergens can be represented by allergen motifs. this observation prompted us to experimentally investigate the synthesized peptides corresponding to the in silico motifs with regard to potential ige binding and cross-reactions with allergens. two motifs were selected as examples to conduct in vitro studies. from the first motif, derived from allergenic mnsod sequences, the motif stretch of the allergen asp f 6 was selected and synthesized as a peptide ... | 2007 | 17303251 |
| immunoglobulin-e-mediated reactivity to self antigens: a controversial issue. | immunoglobulin e (ige) reactivity to self antigens is well established in vitro by elisa, inhibition elisa, western blot analyses and t cell proliferation experiments. in vivo, ige-binding self antigens are able to elicit strong type i reactions in sensitized individuals and, in the case of human manganese superoxide dismutase, to elicit eczematous reactions on healthy skin areas of patients suffering from atopic eczema. the reactions against self antigens sharing structural homology with enviro ... | 2008 | 17823538 |
| in vitro modulation of human keratinocyte pro- and anti-inflammatory cytokine production by the capsule of malassezia species. | malassezia spp. are commensal, cutaneous fungi that are implicated in seborrhoeic dermatitis. we hypothesize that the lipid-rich capsule of malassezia spp. masks the organism from host detection, and depletion of this layer elicits an inflammatory response. to test this, preparations of capsulated or acapsular [10% (v/v) triton x-100 treated], viable and nonviable, exponential or stationary phase malassezia furfur, malassezia globosa, malassezia obtusa, malassezia restricta, malassezia slooffiae ... | 2008 | 18752620 |
| mutational analysis of amino acid residues involved in ige-binding to the malassezia sympodialis allergen mala s 11. | the yeast malassezia sympodialis, which is an integral part of the normal cutaneous flora, has been shown to elicit specific ige- and t-cell reactivity in atopic eczema (ae) patients. the m. sympodialis allergen mala s 11 has a high degree of amino acid sequence homology to manganese superoxide dismutase (mnsod) from homo sapiens (50%) and aspergillus fumigatus (56%). humoral and cell-mediated cross-reactivity between mnsod from h. sapiens and a. fumigatus has been demonstrated. taken together w ... | 2008 | 18922581 |
| auto- and cross-reactivity to thioredoxin allergens in allergic bronchopulmonary aspergillosis. | thioredoxins are cross-reactive allergens involved in the pathogenesis of atopic eczema and asthma. cross-reactivity to human thioredoxin can contribute to the exacerbation of severe atopic diseases. | 2008 | 19032234 |
| global expression profiling in atopic eczema reveals reciprocal expression of inflammatory and lipid genes. | atopic eczema (ae) is a common chronic inflammatory skin disorder. in order to dissect the genetic background several linkage and genetic association studies have been performed. yet very little is known about specific genes involved in this complex skin disease, and the underlying molecular mechanisms are not fully understood. | 2008 | 19107207 |
| isolation of 19 strains of malassezia dermatis from healthy human skin in korea. | this research was conducted on the cultured samples of 160 healthy men and women aged 0-80 years without any skin disease. nineteen clinical isolates of malassezia dermatis showed positive in a catalase test and all grew in 0.5% tween-60 and 0.1% tween-80 added to 2% glucose/1% peptone culture medium. round and ellipsoidal yeast cells and budding of the yeast cells were observed by microscopy, resembling malassezia sympodialis, malassezia furfur and malassezia nana. the 26s rdna polymerase chain ... | 2008 | 19239557 |
| tlr2/myd88-dependent and -independent activation of mast cell ige responses by the skin commensal yeast malassezia sympodialis. | atopic eczema (ae) is a chronic inflammatory skin disease. approximately 50% of adult ae patients have allergen-specific ige reactivity to the skin commensal yeast malassezia spp. due to the ruptured skin barrier in ae, it is likely that malassezia can come into contact with mast cells, which are known to be involved in ae. we therefore hypothesized that malassezia spp. can activate mast cells. bone marrow-derived mast cells (bmmcs) were generated from wild type, tlr2, tlr4, and myd88 gene-delet ... | 2009 | 19299719 |
| elevated peripheral allergen-specific t cell response is crucial for a positive atopy patch test reaction. | atopic eczema is a chronic inflammatory skin disease in which several subgroups of cases can be identified. atopy patch testing (apt) reveals allergen sensitization also in atopic eczema patients devoid of detectable allergen-specific ige, suggesting the importance of factors other than ige in the reaction. here we investigate the relationship between apt reactions and allergen-specific peripheral ige and t cell reactivity in atopic eczema patients. | 2009 | 19339802 |
| identification of (1-->6)-beta-d-glucan as the major carbohydrate component of the malassezia sympodialis cell wall. | members of the genus malassezia are commensal fungi found on the skin of both human and domestic animals and are associated with skin diseases including dandruff/seborrheic dermatitis, pityriasis versicolor, and atopic eczema (ae) in humans. in this study we have characterized the cell-wall carbohydrates of malassezia sympodialis, one of the species most frequently isolated from both ae patients and healthy individuals. cells were grown in liquid dixon media at 32 degrees c, harvested, and proce ... | 2009 | 19853245 |
| glans penis and prepuce colonisation of yeast fungi in a paediatric population: pre- and postcircumcision results. | the members of the genera candida and malassezia comprise opportunistic yeast with a natural habitat on the skin of humans and warm-blooded animals. this study aimed to compare the prevalence of these yeast fungi in samples from the glans penis and prepuce 3-5 min prior to circumcision and after 1-month follow-up by mycological examination. a total of 77 children aged between 0.01 and 13.0 years (mean age 5.8 +/- 3.4) were included in the study. impression preparations were made on modified dixo ... | 2009 | 18498303 |
| molecular identification of malassezia species isolated from dermatitis affections. | the lipophilic yeast of the genus malassezia are opportunistic microorganisms of the skin microflora but they can be agents of various dermatomycoses. the aim of this study was to perform molecular identification of the commonly isolated malassezia species from various dermatomycoses in our region. thirty strains of malassezia were isolated from different dermatologic affections: pityriasis versicolor (17), dandruff (5), seborrheic dermatitis (4), onyxis (2), folliculitis (1) and blepharitis (1) ... | 2009 | 18643889 |
| prevalence of malassezia species in pityriasis versicolor lesions in northeast argentina. | malassezia species normally colonize the skin but they can change their saprophytic state and invade the stratum corneum as pathogens. | 2010 | 20346299 |
| identification of malassezia species from tunisian patients with pityriasis versicolor. | pityriasis versicolor is caused by malassezia sp. it is a common worldwide mycosis. recently, eleven species are known of the malassezia genus, and are identified in vitro by their morphological characteristics, biochemical tests and by molecular biology. the aim of this study is the identification of malassezia species from tunisian patients with pityriasis versicolor. | 2010 | 20415164 |
| [pityriasis versicolor: isolation and identification of the main species of malassezia]. | species of the genus malassezia isolated were: malassezia sympodialis (16.66%), malassezia furfur (12.50%), malassezia globosa (11.45%), and malassezia slooffiae (2.10%). malassezia sympodialis predominated in the study. the species of malassezia identified did not show correlation with clinical variants and with the distribution of pityriasis versicolor lesions in relation to areas of the body. | 2010 | 20464101 |
| pityriasis versicolor circinata: isolation of malassezia sympodialis - case report. | the authors report a case of pityriasis versicolor circinata whose isolated etiologic agent was malassezia sympodialis in a 34-year-old woman. the isolation and identification of malassezia sympodialis were accomplished with modified dixon's agar, and the molecular method used to confirm the species was polymerase chain reaction and restriction fragment length polymorphism analysis (pcr-rflp). | 2010 | 20520940 |
| antimicrobial activity of hymenaea martiana towards dermatophytes and cryptococcus neoformans. | the biological activity of crude extract and fractions of hymenaea martiana was evaluated against a panel of human pathogenic fungi. the crude extracts and hydroalcoholic fractions (e) showed a high activity against cryptococcus neoformans species complex isolates with mics between 2 and 64 μg ml(-1). the methanolic (c) and butanolic (d) fractions were the most active against trichopyton rubrum, trichopyton mentagrophytes and microsporum canis with mics between 8 and 256 μg ml(-1). none of the e ... | 2010 | 19563478 |
| isolation and characterization of malassezia spp. in healthy swine of different breeds. | malassezia spp. genus is represented by several lipophilic yeasts, normally present on the skin of many warm-blooded vertebrates, including man. swine are one of the less investigated animal species. the aim of the present work was to study the occurrence of malassezia spp. in the external ear canal of 408 healthy swine of different breeds, under different breeding conditions. for this purpose n. 185 free-ranging wild boars, n. 107 large size pigs and 116 cinta senese breed were selected. animal ... | 2010 | 19709823 |
| atopic dermatitis in older patients: particular points. | we review the particular characteristics of atopic dermatitis (ad) in adult life, and compare findings with those of ad in childhood. ad affects 1-3% of adults world-wide, and can present as adult-onset ad, or as infantile/childhood ad that persists, or recurs after many years. eczema in adults usually exists for years, compromising quality of life, sex life and occupational choices. the flexural areas, shoulders, head-and-neck, and hands are typically affected. in elderly adults, eczematous ery ... | 2011 | 20569298 |
| mast cells generated from patients with atopic eczema have enhanced levels of granule mediators and an impaired dectin-1 expression. | the disrupted skin barrier of patients with atopic eczema (ae) might facilitate contact between mast cells (mcs) in the skin and environmental triggers of the disease. one such trigger is the skin-colonizing yeast malassezia sympodialis (m. sympodialis). in this study, we investigated the interaction of mc with m. sympodialis. | 2011 | 20608913 |
| prevalencia de especies de malassezia en pacientes con pitiriasis versicolor en rosario, argentina. | malassezia species are considered opportunistic yeasts of increasing clinical importance. these lipophilic yeasts are associated with various human diseases, especially pityriasis versicolor (pv), a chronic superficial scaling dermatomycosis. the aim of this study was to isolate, identify and analyze the distribution of the different species of malassezia in patients with pv in rosario city (argentina). a total of 264 clinical samples were studied. isolates were identified on the basis of micros ... | 2011 | 21419859 |
| malassezia sympodialis thioredoxin-specific t cells are highly cross-reactive to human thioredoxin in atopic dermatitis. | background: ige-mediated cross-reactivity between fungal antigens and human proteins has been described in patients with atopic dermatitis (ad), but it remains to be elucidated whether there is also cross-reactivity at the t-cell level. objective: we sought to explore cross-reactivity at the t-cell level between the fungal thioredoxin (mala s 13) of the skin-colonizing yeast malassezia sympodialis and its homologous human thioredoxin (htrx). methods: t-cell lines (tcls) were generated in the pre ... | 2011 | 21489611 |
| extracellular phospholipase activity of malassezia spp. isolated from individuals with and without dermatological disease. | background: the malassezia genus includes mainly lipophilic yeasts belonging to the cutaneous microbiota of man and other mammals. some malassezia species have been associated with various dermatological diseases. the factors permitting the transformation of yeasts of the malassezia genus from a commensal organism to a pathogenic agent are still little known but the production of various enzymes such as lipase, phospholipase and lipoxygenase could contribute to the pathogenic activity of these y ... | 2011 | 21645629 |
| nanovesicles from malassezia sympodialis and host exosomes induce cytokine responses - novel mechanisms for host-microbe interactions in atopic eczema. | intercellular communication can occur via the release of membrane vesicles. exosomes are nanovesicles released from the endosomal compartment of cells. depending on their cell of origin and their cargo they can exert different immunoregulatory functions. recently, fungi were found to produce extracellular vesicles that can influence host-microbe interactions. the yeast malassezia sympodialis which belongs to our normal cutaneous microbial flora elicits specific ige- and t-cell reactivity in appr ... | 2011 | 21799736 |
| granulomatous dermatitis due to malassezia sympodialis. | a 67-year-old man, with multiple skin lesions that appeared over 2 years, had biopsies that disclosed granulomatous dermatitis with associated small yeasts. the urinary antigen test results were negative for histoplasma infection; cultures from the biopsies did not grow any fungi or other potential pathogens. the chest roentgenogram results were normal. morphologic examination revealed features of a malassezia species. broad-range fungal polymerase chain reaction and dna sequencing disclosed tha ... | 2011 | 21877990 |
| identification of malassezia yeast species isolated from patients with pityriasis versicolor. | pityriasis versicolor (pv) is a disease with worldwide distribution. twelve different species of malassezia yeast have been described. the objective of this study was to determine which species of malassezia are more prevalent in patients with pityriasis versicolor. samples were collected by scraping the lesions of 87 patients with a clinical suspicion of pityriasis versicolor. the samples were then submitted to fungal microscopy and culture to identify the species. the species found were: malas ... | 2011 | 21987156 |
| Cell-penetrating peptides as antifungals towards Malassezia sympodialis. | Aim: To determine whether different antimicrobial peptides (AMPs) and cell-penetrating peptides (CPPs) are able to inhibit the growth of the commensal yeast Malassezia sympodialis, which can act as a trigger factor in different skin disorders, such as atopic eczema (AE), seborrhoeic eczema (SE) and dandruff. Methods and results: The antifungal activity of 21 different AMPs and CPPs was investigated by microdilution assay and plate counting to determine the number of colony forming units. Five ... | 2012 | 22023266 |
| the antifungal effect of light emitting diode on malassezia yeasts. | malassezia (m.) species are members of the normal part of the skin flora, but they might induce or be involved with various cutaneous diseases. although the role of malassezia in the pathogenesis of cutaneous diseases is not fully understood, recent studies have shown that decreased density of malassezia led to improvement of these diseases. | 2012 | 22551719 |
| treatment of atopic dermatitis associated with malassezia sympodialis by green tea extracts bath therapy: a pilot study. | multiple treatment modalities, including topical and systemic corticosteroid and phototherapy, have been used in treatment of patients with atopic dermatitis. however, long-term corticosteroid therapy may have various adverse effects. the purpose of this study was to investigate the therapeutic efficacy and safety of bath therapy using green tea extracts for treatment of patients with atopic dermatitis. a total of four patients with atopic dermatitis were enrolled in this study. a malassezia mul ... | 2012 | 22870055 |
| living and thriving on the skin: malassezia genomes tell the story. | our understanding of the interactions between normal skin microbiota and the human host has been greatly extended by recent investigations. in their recent study in mbio, a. gioti et al. (mbio 4[1]:e00572-12, 2013) sequenced the genome of the atopic eczema-associated yeast, malassezia sympodialis, and compared its gene content and organization with that of malassezia globosa, a species implicated in dandruff. their findings were also contrasted with those previously obtained for ustilago maydis, ... | 2013 | 23512963 |
| genomic insights into the atopic eczema-associated skin commensal yeast malassezia sympodialis. | malassezia commensal yeasts are associated with a number of skin disorders, such as atopic eczema/dermatitis and dandruff, and they also can cause systemic infections. here we describe the 7.67-mbp genome of malassezia sympodialis, a species associated with atopic eczema, and contrast its genome repertoire with that of malassezia globosa, associated with dandruff, as well as those of other closely related fungi. ninety percent of the predicted m. sympodialis protein coding genes were experimenta ... | 2013 | 23341551 |
| sensitization to skin-associated microorganisms in adult patients with atopic dermatitis is of importance for disease severity. | atopic dermatitis (ad) is a chronic inflammatory skin disease. environmental and genetic factors, as well as microbial products from yeasts and bacteria, play a role in triggering the disease. a cohort of 619 adult patients with ad was screened for severity of ad, sensitization to malassezia sympodialis, candida albicans, staphylococcus aureus enterotoxins and dermatophagoides pteronyssinus. serum levels of interleukin (il)-18 were measured. immunoglobulin e (ige) sensitization to the combinatio ... | 2013 | 23073977 |
| epidemiological characteristics of malassezia folliculitis and use of the may-grünwald-giemsa stain to diagnose the infection. | various bacterial, fungal, parasitic, and viral pathogens can cause folliculitis, which is often mistakenly treated with antibiotics for months or even years. a laboratory diagnosis is required before therapy can be planned. here, we describe the prevalence and risk factors, as well as the clinical, cytological, and mycological characteristics, of patients with malassezia folliculitis (mf) in adana, turkey. we also report the treatment responses of the mf patients and describe the malassezia spp ... | 2013 | 23706503 |
| in vitro antifungal activity of topical and systemic antifungal drugs against malassezia species. | the strict nutritional requirements of malassezia species make it difficult to test the antifungal susceptibility. treatments of the chronic and recurrent infections associated with malassezia spp. are usually ineffective. the objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of malassezia species against 16 antifungal drugs used for topical or systemic treatment. isolates were identified by restriction fragment length polymorphism. minimal inhibitory ... | 2013 | 23496653 |
| antifungal susceptibility of malassezia furfur, malassezia sympodialis, and malassezia globosa to azole drugs and amphotericin b evaluated using a broth microdilution method. | we studied the in vitro activity of fluconazole (fcz), ketoconazole (ktz), miconazole (mcz), voriconazole (vcz), itraconazole (itz) and amphotericin b (amb) against the three major pathogenic malassezia species, m. globosa, m. sympodialis, and m. furfur. antifungal susceptibilities were determined using the broth microdilution method in accordance with clinical and laboratory standards institute reference document m27-a3. to support lipid-dependent yeast development, glucose, peptone, ox bile, m ... | 2014 | 24965946 |
| molecular cloning and characterization of a thioredoxin from taiwanofungus camphorata. | thioredoxin (trx) is reduced by thioredoxin reductase. trx is used in ribonucleoide reduction, assimilatory sulfate reduction, in modulation of protein sulfhydryl groups, and refolding proteins. | 2014 | 28510955 |
| distribution of malassezia species on the skin of patients with atopic dermatitis, psoriasis, and healthy volunteers assessed by conventional and molecular identification methods. | the malassezia yeasts which belong to the physiological microflora of human skin have also been implicated in several dermatological disorders, including pityriasis versicolor (pv), atopic dermatitis (ad), and psoriasis (ps). the malassezia genus has repeatedly been revised and it now accommodates 14 species, all but one being lipid-dependent species. the traditional, phenotype-based identification schemes of malassezia species are fraught with interpretative ambiguities and inconsistencies, and ... | 2014 | 24602368 |
| fungemia and interstitial lung compromise caused by malassezia sympodialis in a pediatric patient. | a case of fungemia with interstitial lung compromise caused by malassezia sympodialis is reported in an obese pediatric patient on long-term treatment with inhaled corticosteroids for asthma. the patient was hospitalized due to a post-surgical complication of appendicitis. the patient was treated with amphotericin b for 3 weeks, with good clinical evolution and subsequent negative cultures. | 2015 | 24863142 |
| detection and identification of malassezia species in domestic animals and aquatic birds by pcr-rflp. | the present study aimed at detection and species-level identification of the malassezia yeasts in domestic animals and aquatic birds by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp). samples were collected using tape strips and swabs from 471 animals including 97 horses, 102 cattle, 105 sheep, 20 camels, 60 dogs, 30 cats, 1 hamster, 1 squirrel, 50 aquatic birds and 5 turkeys. tape-strip samples were examined by direct microscopy. all samples were inoculated on mod ... | 2015 | 27175148 |
| compositional and immunobiological analyses of extracellular vesicles released by candida albicans. | the release of extracellular vesicles (ev) by fungal organisms is considered an alternative transport mechanism to trans-cell wall passage of macromolecules. previous studies have revealed the presence of ev in culture supernatants from fungal pathogens, such as cryptococcus neoformans, histoplasma capsulatum, paracoccidioides brasiliensis, sporothrix schenckii, malassezia sympodialis and candida albicans. here we investigated the size, composition, kinetics of internalization by bone marrow-der ... | 2015 | 25287304 |
| malassezia species in healthy skin and in dermatological conditions. | the genus malassezia comprises lipophilic species, the natural habitat of which is the skin of humans and other warm-blooded animals. however, these species have been associated with a diversity of dermatological disorders and even systemic infections. pityriasis versicolor is the only cutaneous disease etiologically connected to malassezia yeasts. in the other dermatoses, such as malassezia folliculitis, seborrheic dermatitis, atopic dermatitis, and psoriasis, these yeasts have been suggested t ... | 2016 | 26710919 |
| gene function analysis in the ubiquitous human commensal and pathogen malassezia genus. | the genus malassezia includes 14 species that are found on the skin of humans and animals and are associated with a number of diseases. recent genome sequencing projects have defined the gene content of all 14 species; however, to date, genetic manipulation has not been possible for any species within this genus. here, we develop and then optimize molecular tools for the transformation of malassezia furfur and malassezia sympodialis using agrobacterium tumefaciens delivery of transfer dna (t-dna ... | 2016 | 27899504 |
| low dna sequence diversity of the intergenic spacer 1 region in the human skin commensal fungi malassezia sympodialis and m. dermatis isolated from patients with malassezia-associated skin diseases and healthy subjects. | as dna sequences of the intergenic spacer (igs) region in the rrna gene show remarkable intraspecies diversity compared with the small subunit, large subunit, and internal transcribed spacer region, the igs region has been used as an epidemiological tool in studies on malassezia globosa and m. restricta, which are responsible for the exacerbation of atopic dermatitis (ad) and seborrheic dermatitis (sd). however, the igs regions of m. sympodialis and m. dermatis obtained from the skin of patients ... | 2016 | 27371104 |
| ige sensitization profiles differ between adult patients with severe and moderate atopic dermatitis. | atopic dermatitis (ad) is a complex chronic inflammatory disease where allergens can act as specific triggering factors. | 2016 | 27228091 |