Publications

genetic variability within the aspergillus viridinutans species.	the intraspecific variability of the aspergillus viridinutans species was examined using various techniques including morphological examinations, carbon-source-utilization tests, restriction-enzyme analysis of the mitochondrial and nuclear dna, and sequence analysis of part of the beta-tubulin gene. although the genetic distances between the isolates were higher than between, e.g., a. fumigatus and neosartorya fischeri, most a. viridinutans isolates, together with n. aureola and n. udagawae stra ...	2000	11347269
multiple genetically distinct groups revealed among clinical isolates identified as atypical aspergillus fumigatus.	to investigate whether genetic variants of a. fumigatus are found among clinical isolates, four isolates that were originally identified as poorly sporulating strains of aspergillus fumigatus were subjected to molecular analysis. dna sequence analysis of the alkaline protease genes of these isolates showed that each is genetically distinct and each shows substantial variation (7 to 11%) from the a. fumigatus nucleotide sequence. subsequent morphological examination suggested that all of the isol ...	2005	15695644
isolation, characterization and optimization of culture parameters for production of an alkaline protease isolated from aspergillus tamarii.	aspergillus tamarii expresses an extracellular alkaline protease that we show to be effective in removing hair from cattle hide. large quantities of the enzyme will be required for the optimization of the enzymatic dehairing process so the growth conditions for maximum protease expression by a. tamarii were optimized for both solid-state culture on wheat bran and for broth culture. optimal protease expression occurred, for both cultural media, at initial ph 9; the culture was incubated at 30 deg ...	2007	17245587
aspergillus section fumigati: antifungal susceptibility patterns and sequence-based identification.	this study analyzed 28 aspergillus strains belonging to the section fumigati that were isolated from clinical samples in spain. all isolates sporulated slowly and were unable to grow at 48 degrees c. phylogenetic analysis based on sequencing of partial sequences of the beta-tubulin and rodlet a genes was used to classify the 28 strains into six different clades (neosartorya hiratsukae, neosartorya pseudofischeri, aspergillus viridinutans, aspergillus lentulus, aspergillus fumigatiaffinis, and as ...	2008	18212093
chronic invasive aspergillosis caused by aspergillus viridinutans.	aspergillus viridinutans, a mold phenotypically resembling a. fumigatus, was identified by gene sequence analyses from 2 patients. disease was distinct from typical aspergillosis, being chronic and spreading in a contiguous manner across anatomical planes. we emphasize the recognition of fumigati-mimetic molds as agents of chronic or refractory aspergillosis.	2009	19751595
rapid identification of aspergillus fumigatus within the section fumigati.	abstract:	2011	21510879
aspergillus viridinutans: an agent of adult chronic invasive aspergillosis.	in contrast with the common hematogenous dissemination of invasive aspergillosis (ia), we present case with a protracted course through anatomical planes in an immunocompromised adult male. the unusual clinical features and laboratory findings led to fungal genotyping and identification of the mold as aspergillus viridinutans. it appears to be the first described case of ia caused by this agent in an adult patient.	2011	21299372
second-generation synthesis of (-)-viriditoxin.	viriditoxin is a secondary metabolite isolated from aspergillus viridinutans that has been shown to inhibit ftsz, the bacterial homologue of eukaryotic tubulin. a streamlined, scalable, and highly diastereoselective synthesis of this complex natural product is described. key advances include a more efficient synthesis of the requisite unsaturated pyranone, scalable assembly of the naphthopyranone monomer, and improved diastereoselectivity in the biaryl-coupling reaction. in addition, we disclose ...	2012	23049144
keratomycosis caused by aspergillus viridinutans: an aspergillus fumigatus-resembling mold presenting distinct clinical and antifungal susceptibility patterns.	we report here a case of fungal keratitis caused by aspergillus viridinutans, a member of the genus which was found to have clinical and antifungal susceptibility characteristics distinct from the species it most closely resembles, aspergillus fumigatus. a 26-year-old woman presented with contact lens-associated keratitis, the etiologic agent of which was initially slow growing and found to be resistant to amphotericin b and voriconazole. therapeutic keratoplasty was performed and an isolate fro ...	2012	22329455
[invasive fungal infections in immunocompromised patients with focus on aspergillosis and its causative agents].	invasive fungal diseases (ifd) are a life-threatening infectious complications in immunocompromised patients and are associated with high rate of morbidity and mortality. the most common invasive mycosis in patients who underwent an allogeneic hematopoietic stem cell transplantation is invasive aspergilosis (ia), most frequently caused by the clinically dominant species aspergillus fumigatus and, rarely, also by aspergillus flavus, aspergillus terreus and aspergillus niger. in recent years, othe ...	2012	23172020
diversity and specificity of microsatellites within aspergillus section fumigati.	microsatellites (or short tandem repeats, strs) are the genetic markers of choice for studying aspergillus fumigatus molecular epidemiology due to its reproducibility and high discrimination power. however, the specificity of these markers must be investigated in a group of isolates from closely related species. the aim of this work was to test a microsatellite-based pcr multiplex previously designed for a. fumigatus in a set of species belonging to section fumigati, namely aspergillus fumigatia ...	2012	22838495
new insights concerning the occurrence of fungi in water sources and their potential pathogenicity.	fungi are known to occur ubiquitously in the environment. in the past years, the occurrence of filamentous fungi in the aquatic environment has been a subject of growing interest. this study describes the occurrence of various fungal genera in different drinking water sources being penicillium and trichoderma the most representative ones (30% and 17%, respectively). also, 24 fungal species that have not been previously described in the aquatic environment are reported in this study, being once a ...	2013	24011405
invasive aspergillosis caused by cryptic aspergillus species: a report of two consecutive episodes in a patient with leukaemia.	we report a case of two consecutive episodes of invasive aspergillosis caused by cryptic aspergillus species in a patient with leukaemia. a first episode of pulmonary infection was caused by aspergillus calidoustus and aspergillus novofumigatus, and the second episode by a. novofumigatus and aspergillus viridinutans. fungal isolates were identified to species level using traditional and sequencing-based molecular methods.	2013	23161769
multi-resistant aspergillosis due to cryptic species.	reports of cryptic species causing aspergillosis in humans are increasing in the literature. cryptic species are defined as those which are morphologically indistinguishable, although their identifications can be confirmed using molecular or other techniques which continue to become more widely available in the clinical setting. antifungal resistance has often been noted in these cases, and indeed there does appear to be a higher prevalence of reduced susceptibility in cryptic species. many of t ...	2014	24969615
[a case of lung abscess due to aspergillus viridinutans in a patient with aplastic anemia].	a 75-year-old woman with aplastic anemia was admitted to our university hospital because of a dry cough that had persisted for a month. chest computed tomography showed a mass shadow with a central low attenuation area in the lower lobe of the left lung. filamentous fungus resembling aspergillus fumigatus was cultured from the specimens obtained by transthoracic needle aspiration biopsy and bronchoalveolar lavage. the initial diagnosis was a lung abscess due to a. fumigatus, although the patient ...	2014	25764808
polyphasic characterization of fungal isolates from a published case of invasive aspergillosis reveals misidentification of aspergillus felis as aspergillus viridinutans.		2014	24469682
secondary metabolite profiles and antifungal drug susceptibility of aspergillus fumigatus and closely related species, aspergillus lentulus, aspergillus udagawae, and aspergillus viridinutans.	the incidence of aspergillus infection has been increasing in the past few years. also, new aspergillus fumigatus-related species, namely aspergillus lentulus, aspergillus udagawae, and aspergillus viridinutans, were shown to infect humans. these fungi exhibit marked morphological similarities to a. fumigatus, albeit with different clinical courses and antifungal drug susceptibilities. the present study used liquid chromatography/time-of-flight mass spectrometry to identify the secondary metabol ...	2015	25737146
aspergillus fumigatus-related species in clinical practice.	aspergillus fumigatus is the main etiologic agent of invasive aspergillosis (ia). other aspergillus species belonging to the section fumigati (a. fumigatus complex) may occasionally be the cause of ia. these strains are often misidentified, as they cannot be distinguished from a. fumigatus by conventional morphological analysis and sequencing methods. this lack of recognition may have important consequences as these a. fumigatus-related species often display some level of intrinsic resistance to ...	2016	27242710
one-health pathogens in the aspergillus viridinutans complex.	cryptic species in aspergillus section fumigati are increasingly recognised as pathogens in humans and animals. the a. viridinutans complex (avc) has recently expanded to comprise 10 species, of which six are known to be pathogenic, including a. udagawae, a. felis, a. pseudofelis, a. parafelis, a. pseudoviridinutans, and a. wyomingensis. they cause locally invasive and disseminated invasive disease syndromes, including chronic pulmonary aspergillosis and invasive aspergillosis in humans, invasiv ...	2017	28379569
discovery of aspergillus frankstonensis sp. nov. during environmental sampling for animal and human fungal pathogens.	invasive fungal infections (ifi) due to species in aspergillus section fumigati (asf), including the aspergillus viridinutans species complex (avsc), are increasingly reported in humans and cats. the risk of exposure to these medically important fungi in australia is unknown. air and soil was sampled from the domiciles of pet cats diagnosed with these ifi and from a nature reserve in frankston, victoria, where aspergillus viridinutans sensu stricto was discovered in 1954. of 104 asf species isol ...	2017	28792943