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gracilibacillus gen. nov., with description of gracilibacillus halotolerans gen. nov., sp. nov.; transfer of bacillus dipsosauri to gracilibacillus dipsosauri comb. nov., and bacillus salexigens to the genus salibacillus gen. nov., as salibacillus salexigens comb. nov.a gram-positive, extremely halotolerant bacterium was isolated from the great salt lake, utah, usa. the strain, designated nnt (= dsm 11805t), was strictly aerobic, rod-shaped, motile by peritrichous flagella and spore-forming. strain nnt grew at salinities of 0-20% (w/v) nacl. a distinctive feature of strain nnt was its optimal growth in salt-free medium. the polar lipid pattern of strain nnt consisted of phosphatidyl glycerol, diphosphatidyl glycerol and two phospholipids of unknown structure. ...199910319508
paenibacillus jamilae sp. nov., an exopolysaccharide-producing bacterium able to grow in olive-mill wastewater.endospore-forming strains were isolated from corn-compost treated with olive-mill wastewater ('alpechin'). the strains were taxonomically studied and proposed as a novel paenibacillus species. these organisms (strains b.3t, b.7 and b.9) were particularly distinguishable from other aerobic spore-forming species by their ability to grow optimally in 100% (v/v) olive-mill wastewater at 30 degrees c and ph 7.0 and concomitant production of an interesting exopolysaccharide. chemotaxonomic analysis re ...200111594596
[amphibacillus fermentum sp. nov., amphibacillus tropicus sp. nov.--new alkaliphilic, facultatively anaerobic, saccharolytic bacilli from lake magadi].new alkaliphilic, saccharolytic, rod-shaped, gram-positive bacteria resistant to heating and drying and phylogenetically affiliated to the bacillus lineage were isolated under strictly anaerobic conditions from sediments of the alkaline and highly mineralized lake magadi. strain z-7792 forms endospores; in strain z-7984, endospore formation was not revealed. the strains are capable of both anaerobic growth (at the expense of fermentation of glucose and certain mono- and disaccharides with the fo ...200111785140
paraliobacillus ryukyuensis gen. nov., sp. nov., a new gram-positive, slightly halophilic, extremely halotolerant, facultative anaerobe isolated from a decomposing marine alga.a slightly halophilic, extremely halotolerant, alkaliphilic, and facultatively anaerobic rod bacterium was isolated from a decomposing marine alga collected in okinawa, japan. the isolate, designated o15-7(t), was gram-positive, endospore-forming, catalase-positive, menaquinone-7-possessing bacterium that is motile by peritrichous flagella. the isolate was an inhabitant of marine environments; the optimum nacl concentration for growth was 0.75-3.0% (w/v) with a range of 0-22.0%, and the optimum ...200212501437
pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic gram-positive bacterium from a solar saltern in korea.three moderately halophilic, spore-forming strains, designated bh030062t, bh030049 and bh030080, were isolated from a solar saltern in korea. phylogenetic analyses and comparative 16s rrna gene sequence studies revealed that the isolates represent a novel distinct monophyletic lineage within the phyletic group classically defined as the genus bacillus and are most closely related to members of the genera gracilibacillus (93.7-95.1 % similarity), virgibacillus (93.5-94.8 %), halobacillus (94.8-95 ...200515653871
endospores of halophilic bacteria of the family bacillaceae isolated from non-saline japanese soil may be transported by kosa event (asian dust storm).generally, extremophiles have been deemed to survive in the extreme environments to which they had adapted to grow. recently many extremophiles have been isolated from places where they are not expected to grow. alkaliphilic microorganisms have been isolated from acidic soil samples with ph 4.0, and thermophiles have been isolated from samples of low temperature. numerous moderately halophilic microorganisms, defined as those that grow optimally in media containing 0.5-2.5 molar (3-15%) nacl, an ...200516242015
halolactibacillus halophilus gen. nov., sp. nov. and halolactibacillus miurensis sp. nov., halophilic and alkaliphilic marine lactic acid bacteria constituting a phylogenetic lineage in bacillus rrna group 1.eleven novel strains of marine-inhabiting lactic acid bacteria that were isolated from living and decaying marine organisms collected from a temperate area of japan are described. the isolates were motile with peritrichous flagella and non-sporulating. they lacked catalase, quinones and cytochromes. fermentation products from glucose were lactate, formate, acetate and ethanol. lactate yield as percentage conversion from glucose was affected by the ph of the fermentation medium: approximately 55 ...200516280507
gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in inner mongolia, china.three gram-positive, moderately halophilic, spore-forming rods, designated xh-63t, xh-62 and ej-15, were isolated from two salt lakes located near xilin hot and ejinor, in inner mongolia, china. the strains were strictly aerobic and motile, with spherical, terminal and deforming endospores. they grew at ph 5.0-9.0 (optimal growth at ph 7.5), between 4 and 45 degrees c (optimal growth at 37 degrees c) and at salinities of 1-20% (w/v) total salts, growing optimally at 10% (w/v) salts. they had mes ...200616514034
gracilibacillus boraciitolerans sp. nov., a highly boron-tolerant and moderately halotolerant bacterium isolated from soil.a motile, gram-positive, boron-tolerant and moderately halotolerant rod-shaped bacterium was isolated from a soil naturally high in boron minerals found in the hisarcik area of turkey. the novel isolate, designated t-16x(t), produced spherical or ellipsoidal endospores in a non-bulging or slightly swollen sporangium in a terminal position and survived in a medium containing up to 450 mm boron. whereas it tolerated 11 % (w/v) nacl, it also grew without nacl or boron. the temperature range for gro ...200717392209
biodecolorization of azo dye acid red b under high salinity condition.the study was conducted by a novel salt tolerant bacterium gracilibacillus sp. gty. the strain was identified on the basis of morphological and physio-biochemical characteristics, and 16s rdna sequence analysis. decolorization was performed by growing and resting cells, as well as by extracted azo reductase. strain grown in the media containing 15% (w/v) of nacl showed the best performance in decolorization. decolorization was observed by the uv-visible absorbance spectra. the maximum absorption ...200717721733
changes in bacterial communities accompanied by aggregation in a fed-batch composting reactor.the contents of fed-batch composting (fbc) reactors often aggregate after prolonged operation. this process leads to irreversible breakdown of the decomposition reaction and possible alteration of the bacterial communities. we compared the structures of bacterial communities in reactors under aggregate and optimal conditions. the results of 16s rrna gene clone analysis showed that populations of the family bacillaceae (such as bacillus spp., cerasibacillus spp., gracilibacillus spp.), which domi ...200818231830
gracilibacillus quinghaiensis sp. nov., isolated from salt-lake sediment in the qaidam basin, north-west china.a motile, gram-positive, slightly halophilic, endospore-forming, catalase- and oxidase-positive, obligately aerobic, slender rod-shaped bacterium, strain yim-c229t was isolated from the sediment of a salt lake in the qaidam basin, north-west china. filamentous forms were present throughout the growth cycle. strain yim-c229t grew in the presence of 0.5-8% nacl and at ph 6.0-8.5, with optimum growth at 1-3% nacl and ph 7.0-7.5. it grew at 4-45 degrees c, with optimum growth at 37 degrees c. the ma ...200818571887
gracilibacillus lacisalsi sp. nov., a halophilic gram-positive bacterium from a salt lake in china.two gram-positive, motile, rod-shaped, halophilic bacteria showing optimum growth at 5-7 % (w/v) nacl were isolated from a salt lake, xin-jiang province, china. the strains, designated bh312(t) and bh314, grew in the presence of 1-18 % (w/v) nacl and at temperatures of 15-50 degrees c (optimum: 40 degrees c) and ph values of 5.5-10.0 (optimum: ph 7.5-8.0). the major cellular fatty acids were anteiso-c(15 : 0), iso-c(15 : 0) and anteiso-c(17 : 0). the dna g+c content was 38.8-39.0 mol% and the pr ...200818842841
gracilibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from saline soil.a gram-positive, moderately halophilic, moderately thermophilic, endospore-forming, catalase- and oxidase-positive, obligately aerobic bacterium, strain yim-c55.5(t), was isolated from saline soil in the qaidam basin, north-west china. cells were slender rods, motile by means of peritrichous flagella. filamentous forms were present throughout the growth cycle. strain yim-c55.5(t) was able to grow in the presence of 7-30 % (w/v) nacl and at ph 6.0-9.0, with optimum growth at 15 % (w/v) nacl and p ...200818842864
isolation and characterization of alginate-degrading bacteria for disposal of seaweed wastes.isolation of novel alginate degrading bacteria for the disposal of seaweed waste in composting process.200919018967
screening and isolation of halophilic bacteria producing extracellular hydrolyses from howz soltan lake, iran.screening of bacteria from different areas of howz soltan playa, a hypersaline lake in the central desert zone of iran, led to the isolation of 231 moderately halophilic bacteria, which were able to grow optimally in media with 5-15% of salt, and 49 extremely halophilic microorganisms that required 20-25% of salt for optimal growth. these isolates produced a great variety of extracellular hydrolytic enzymes. a total of 195, 177, 100, 95, 92, 68, 65, 33, and 28 strains produced lipases, amylases, ...200919037673
diversity and phylogeny of culturable spore-forming bacilli isolated from marine sediments.members of the genus bacillus and related genera are ubiquitous in nature. however, bacillus species isolated from marine sediments have attracted less interest respect to their terrestrial relatives. here, we report the phylogenetic diversity of a collection of 96 bacilli, isolated from 17 distinct stations of 5 oceanographic campaigns. the diversity was analysed by phenotypic and molecular approaches based on the amplified rdna restriction analysis (ardra), amplification of the internal transc ...200919322832
gracilibacillus saliphilus sp. nov., a moderately halophilic bacterium isolated from a salt lake.a novel gram-positive-staining, aerobic, spore-forming, rod-shaped bacterium, designated strain yim 91119(t), was isolated from ebinur lake in xinjiang province, north-west china. cells were motile, produced terminal endospores and grew at ph 6.0-8.0 (optimally at ph 7.0), 4-45 degrees c (optimally at 28-37 degrees c) and 1-22 % (w/v) nacl, (optimally at 10-15 %, w/v). comparative 16s rrna gene sequence analysis showed that strain yim 91119(t) belongs to the genus gracilibacillus, exhibiting the ...200919542126
gracilibacillus thailandensis sp. nov., from fermented fish (pla-ra).a novel strain, designated tp2-8(t), was isolated from fermented fish (pla-ra) in thailand. it stained gram-positive and the cells were aerobic, endospore-forming rods. the strain grew at ph 6-8 (optimum ph 7), 15-55 degrees c (optimum 37 degrees c) and 1-22 % (w/v) nacl (optimum 5-10 %). it contained meso-diaminopimelic in the cell-wall peptidoglycan. mk-7 and cellular fatty acids anteiso-c(15 : 0), iso-c(15 : 0) and anteiso-c(17 : 0) were major components. polar lipids diphosphatidylglycerol a ...201019661518
gracilibacillus ureilyticus sp. nov., a halotolerant bacterium from a saline-alkaline soil.a gram-stain-positive, halotolerant, neutrophilic, rod-shaped bacterium, strain mf38(t), was isolated from a saline-alkaline soil in china and subjected to a polyphasic taxonomic characterization. the isolate grew in the presence of 0-15 % (w/v) nacl and at ph 6.5-8.5; optimum growth was observed with 3.0 % (w/v) nacl and at ph 7.0. chemotaxonomic analysis showed menaquinone mk-7 as the predominant respiratory quinone and anteiso-c(15 : 0), anteiso-c(17 : 0), iso-c(15 : 0), c(17 : 0) and c(16 : ...201019671721
mechanism of boron tolerance in soil bacteria.boron (b) is toxic to living cells at levels above a certain threshold. we isolated several b-tolerant bacterial strains from soil samples and studied them for possible mechanisms of b tolerance. 16s rrna gene sequencing and comparative phylogenetic analysis demonstrated that the isolates belong to the following 6 genera: arthrobacter, rhodococcus, lysinibacillus, algoriphagus, gracilibacillus, and bacillus. these isolates exhibited b-tolerance levels of 80, 100, 150, 300, 450, and 450 mmol/l, r ...201020130690
bacterial community in the personal-use composting reactor revealed by isolation and cultivation-independent method.composting is an efficient and cost-effective process for organic waste treatment. in order to expand our knowledge regarding microorganisms and their roles in the composting process, bacterial community structures in the personal-use composting reactor were examined by isolation and 16s rdna clone analysis (cultivation-independent method). the results of 16s rdna clone analysis showed that populations of the bacillaceae family (such as bacillus spp., cerasibacillus spp., gracilibacillus spp.), ...201020512727
improved composting of undaria pinnatifida seaweed by inoculation with halomonas and gracilibacillus sp. isolated from marine environments.composting of the undaria pinnatifida (wakame) seaweed was conducted after inoculation with 6×10(8) cfu g(-1)halomonas sp. aw4 and the alginate-degrading bacterium gracilibacillus sp. a7. inoculation with strains a7 and aw4 resulted in 27.8% and 24.7% degradation of u. pinnatifida dry mass after 168 h, whereas only 17.5% degradation occurred in the uninoculated control. the c/n ratio decreased in the a7 and aw4 inoculated compost by 7.0% and 9.2% after 72 h, but increased by 11.5% in the control ...201021145233
gracilibacillus kekensis sp. nov., a novel moderate halophile isolated from keke salt lake in qinghai, china.a novel moderate halophilic bacterium, designated strain k170t, was isolated from keke salt lake in qinghai, china. the strain grew with 0-22% (w/v) nacl, at 4-50 -¦c and ph 6-11, with an optimum of 3% (w/v) nacl, 40 -¦c and ph 8, respectively. the predominant respiratory quinone was menaquinone 7 (mk-7). polar lipids diphosphatidylglycerol, phosphatidylglycerol and unidentified lipids were detected. the major cellular fatty acids were anteiso-c15:0, anteiso-c17:0, iso-c15:0 and c16:0. the dna g ...201121685255
characterization of alginase and elicitor-active oligosaccharides from gracilibacillus a7 in alleviating salt stress for brassica campestris l.alginase was purified from gracilibacillus a7 and evaluated for its ability to produce elicitor-active oligosaccharides. the optimum conditions for the alginase reaction are as follows: temperature, 40 -¦c; ph, 8.0; alginate content, 0.3-0.7%; and the presence of na(+) and mg(2+) metal ions. the degree of polymerization (dp) decreased as the reaction time of the alginase progressed, achieving values of 5.4 and 3.3 after 240 and 300 min, respectively. the relative root length (rrl) of the brassic ...201121696216
gracilibacillus bigeumensis sp. nov., a moderately halophilic bacterium from a solar saltern soil.a gram-positive, moderately halophilic bacterium, designated strain bh097(t), was isolated from a solar saltern soil of bigeum island in south-west korea. cells were motile rods, producing spherical endospores at a terminal position in swollen sporangia. strain bh097(t), a strictly aerobic bacterium, grew between ph 5.5 and 9.5 (optimal growth at ph 8.0), between 10 and 52 °c (optimal growth at 37 °c) and at salinities of 1-22 % (w/v) nacl, growing optimally at 7 % (w/v) nacl. on the basis of 16 ...201121984665
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