Publications

a small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for pcr assay.	the complexity of most nucleic acid extraction procedures limits the number of samples that can be easily processed for analysis by polymerase chain reaction (pcr). a simple, small-scale procedure was developed which can be carried out entirely in 1.5-ml microfuge tubes whereby the container and contents are frozen with liquid nitrogen, tissue is pulverized, and targeted nucleic acids are extracted. dna of bacterial and phytoplasmal plant pathogens was extracted in hot ctab buffer followed by ch ...	1998	9628220
cdna cloning and molecular characterization of cherry green ring mottle virus.	the complete nucleotide sequence of the cherry green ring mottle virus (cgrmv) genome was determined to be 8372 nt excluding a 3' poly(a) tail. based on computer analysis and sequence comparison, five open reading frames (orfs) were identified on the virion strand encoding: a putative rna-dependent rna polymerase, a triple gene block and a coat protein. two other orfs with mr values over 10,000 and internal to the helicase and coat protein genes, but of unknown function, were also identified. se ...	1998	9747738
complete nucleotide sequence of cherry necrotic rusty mottle virus.	a virus closely related to cherry green ring mottle virus (cgrmv) was isolated from a tree displaying typical symptoms of cherry necrotic rusty mottle disease. we have named this virus cherry necrotic rusty mottle virus (cnrmv) and report here its complete genomic sequence as determined from overlapping cloned cdnas. cnrmv has a genome of 8,432 nucleotides excluding the 3' poly(a) sequence and codes for 7 significant open reading frames (orfs). five of these orfs are conserved among all fovea-, ...	2001	11315647
the genome organisation and taxonomy of sugarcane striate mosaic associated virus.	sugarcane striate mosaic associated virus (scsmav) has slightly flexuous 950 nm x 15 nm filamentous particles and is associated with sugarcane striate mosaic disease in central queensland, australia. we report the full sequence of its rna genome, which comprises 5 open reading frames representing the polymerase, movement function proteins encoded in a triple gene block and coat protein. phylogenetic analyses based on either the full nucleotide sequence, the polymerase protein, or the coat protei ...	2001	11676409
molecular characterization of foveaviruses associated with the cherry necrotic mottle leaf disease and complete sequencing of an european isolate of cherry green ring mottle virus.	analysis of the p1c124 source (associated with the cherry necrotic mottle leaf (cnml) disease) revealed the presence of two different viral agents. the complete nucleotide sequence of one of these agents, p1a, had an overall nucleotide sequence similarity of 83% with a previously sequenced north american isolate of cherry green ring mottle virus (cgrmv) and should therefore be regarded as an european isolate of cgrmv. approximately 2 kb of the 5' end of the genome of the second agent, p1b, were ...	2002	12021871
an improved reverse transcription-polymerase chain reaction (rt-pcr) assay for the detection of two cherry flexiviruses in prunus spp.	a one-step reverse transcription-polymerase chain reaction (rt-pcr) procedure was developed to detect cherry green ring mottle virus (cgrmv) and cherry necrotic rusty mottle virus (cnrmv) in woody indicators and naturally infected prunus spp. viral rna suitable for rt-pcr was obtained by a simple trapping method that did not require either extraction of double-stranded rna (dsrna) or total rna, availability of virus antibodies, or purification of viral particles. consensus primers, degenerate pr ...	2005	15996763
partial genome sequence of an apricot isolate of cherry green ring mottle virus (cgrmv). annotated sequence record.		2005	15526145
reverse transcription-polymerase chain reaction-based detection of plant viruses.	a one-step reverse transcription-polymerase chain reaction (rt-pcr) is used to detect two cherry flexiviruses, cherry green ring mottle virus (cgrmv) and cherry necrotic rusty mottle virus (cnrmv), in prunus species. this unit presents procedures for collection of plant samples, preparation of total nucleic acids, viral rna-rich or total rna extracts from plant tissues, and subsequent amplification of the viral targets by one-step rt-pcr using a pair of consensus primers. the pcr amplicons are v ...	2007	18770613
the capsid protein of grapevine rupestris stem pitting-associated virus contains a typical nuclear localization signal and targets to the nucleus.	grapevine rupestris stem pitting-associated virus (grspav) is a positive strand, ssrna virus of the genus foveavirus (family betaflexiviridae; order tymovirales). grspav is distributed in table and wine grapes worldwide and comprises a large family of sequence variants. as a newly discovered virus, mechanisms of virus replication and movement of grspav have not been elucidated. we recently revealed the subcellular localization of the proteins encoded by the triple gene block of grspav (rebelo et ...	2010	20708048
a duplex, sybr green i-based rt-qpcr assay for the simultaneous detection of apple chlorotic leaf spot virus and cherry green ring mottle virus in peach.	co-infections of apple chlorotic leaf spot virus (aclsv) and cherry green ring mottle virus (cgrmv) in peach is common in china and have resulted in significant yield reductions. a reliable, sensitive and quantitive method is needed to detect and distinguish between aclsv and cgrmv in peach.	2013	23938201
a one-step multiplex rt-pcr assay for simultaneous detection of four viruses that infect peach.	a multiplex reverse transcription polymerase chain reaction (mrt-pcr) assay was developed to enable the simultaneous detection and differentiation of four viruses that infect peach, namely apple chlorotic leaf spot virus (aclsv), cherry green ring mottle virus (cgrmv), prunus necrotic ringspot virus (pnrsv) and apricot pseudo-chlorotic leaf spot virus (apclsv). in this study, four pairs of primers, one specific for each virus, were designed; the corresponding pcr products were 632, 439, 346 and ...	2013	23777367
viruses associated with rusty mottle and twisted leaf diseases of sweet cherry are distinct species.	virus rna sequences related to those of the family betaflexiviridae were amplified from trees affected with the following diseases: cherry twisted leaf, apricot ring pox, cherry necrotic rusty mottle, cherry rusty mottle, and cherry green ring mottle. phylogenetic analysis of virus sequences obtained from these diseased trees from western north america, along with published sequences of cherry green ring mottle virus (cgrmv) and cherry necrotic rusty mottle virus (cnrmv), revealed four major cla ...	2013	24219146
complete nucleotide sequence of a virus associated with rusty mottle disease of sweet cherry (prunus avium).	cherry rusty mottle is a disease of sweet cherries first described in 1940 in western north america. because of the graft-transmissible nature of the disease, a viral nature of the disease was assumed. here, the complete genomic nucleotide sequences of virus isolates from two trees expressing cherry rusty mottle disease symptoms are characterized; the virus is designated cherry rusty mottle associated virus (crmav). the biological and molecular characteristics of this virus in comparison to thos ...	2013	23525699
complete nucleotide sequences of two isolates of cherry green ring mottle virus from peach (prunus persica) in china.	two complete nucleotide sequences of cherry green ring mottle virus (cgrmv) isolated from peach in hebei (hs10) and fujian (f9) provinces, china, were determined. five open reading frames (orfs) were found in the genomes of both isolates. the f9 and hs10 isolates shared 82.2 % and 83.4-94.4 % nucleotide sequence identity, respectively, with two cgrmv isolates from cherry. analysis of the nucleotide and amino acid sequences from the five orfs of both isolates showed that hs10 shares the greatest ...	2013	23108577
simultaneous detection of cherry necrotic rusty mottle virus and cherry green ring mottle virus using real-time pcr and high resolution melting analysis.	in this study, the real-time pcr assays were combined with high resolution melting (hrm) analysis for the simultaneous detection of cherry necrotic rusty mottle virus (cnrmv) and cherry green ring mottle virus (cgrmv) infection in sweet cherry trees. detection of cnrmv and cgrmv was performed in a real-time pcr using a primer set for both of them or duplex real-time pcr that included one specific primer set for each virus. these two strategies allowed us to confirmed virus infection in all teste ...	2014	24675146
survey of cherry necrotic rusty mottle virus and cherry green ring mottle virus incidence in korea by duplex rt-pcr.	the incidence of cherry necrotic rusty mottle virus (cnrmv) and cherry green ring mottle virus (cgrmv) have recently been occurred in korea, posing a problem for sweet cherry cultivation. since infected trees have symptomless leaves or ring-like spots on the pericarp, it is difficult to identify a viral infection. in this study, the incidence of cnrmv and cgrmv in sweet cherry in gyeongbuk province was surveyed using a newly developed duplex reverse transcriptase polymerase chain reaction (rt-pc ...	2014	25506311
genomic analyses of cherry rusty mottle group and cherry twisted leaf-associated viruses reveal a possible new genus within the family betaflexiviridae.	it is demonstrated that closely related viruses within the family betaflexiviridae are associated with a number of diseases that affect sweet cherry (prunus avium) and other prunus spp. cherry rusty mottle-associated virus (crmav) is correlated with the appearance of cherry rusty mottle disease (crmd), and cherry twisted leaf-associated virus (ctlav) is linked to cherry twisted leaf disease (ctld) and apricot ringpox disease (arpd). comprehensive analysis of previously reported full genomic sequ ...	2015	25496302