Publications

colletotrichum circinans as a semiquantitative test unit for the growth substance produced by rhizopus suinus.		1938	17773760
agencies affecting the production of substance b by rhizopus suinus.		1941	16653716
growth of rhizopus suinus as affected by certain growth factors.		1942	16653774
cerebral mucormycosis: pathogenesis of the disease; description of the fungus, rhizopus oryzae, isolated from a fatal case.		1955	14361464
the effects of metabolic alterations on experimental rhizopus oryzae (mucormycosis) infection.		1956	13361273
the effects of cortisone and chemical inflammation on experimental mucormycosis (rhizopus oryzae infection).		1957	13423366
leukopenia with granulocytopenia in experimental mucormycosis (rhizopus oryzae infection).	mucormycosis was produced in rabbits with sustained, severe leukopenia and granulocytopenia induced by repeated injections of nitrogen mustard. initially, these animals developed extensive fungus lesions at the site of inoculation which later became granulomatous and tended to heal. only the early phases of host resistance appeared impaired by the virtual elimination of the polymorphonuclear leukocyte as a factor in the host response. despite the persistent leukopenia and granulocytopenia, the l ...	1957	13475609
activation of quiescent mucormycotic granulomata in rabbits by induction of acute alloxan diabetes.	in normal rabbits subcutaneous granulomata produced by the injection of a spore suspension of rhizopus oryzae remained confined to the site of inoculation, showed no fungus proliferation, no longer yielded the agent on culture 10 weeks after inoculation, and eventually healed. similar well established granulomata in rabbits with acute alloxan diabetes induced 8, 10, and 15 days after injection of the fungus uniformly showed activation of the infection. this occurred only in animals showing aceto ...	1958	13549649
the inhibitory effect of amphotericin b on localized rhizopus oryzae infection (mucormycosis) utilizing the pneumoderma pouch of the rat.		1958	24544973
treatment of experimental mucormycosis (rhizopus oryzae infection) in rabbits with amphotericin b.		1958	24544889
production of fumaric acid by rhizopus arrhizus.		1959	13637887
gas exchange in rhizopus oryzae.		1960	13826036
studies of opportunistic fungi. i. inhibition of rhizopus oryzae by human serum.		1961	13703046
production of fumaric acid in 20-liter fermentors.	the conditions necessary for the production of fumaric acid in 20-liter fermentors by fermentation of glucose with rhizopus arrhizus strain nrrl 2582 were determined. continuous neutralization of fumaric acid was necessary for optimal yields. yields of the calcium salt were in excess of 65 g of fumaric acid from 100 g of sugar consumed during fermentation of sugar concentrations of 10 to 16%. conditions established for calcium fumarate production include a simple mineral salts medium, 0.5 v:v:mi ...	1962	16349614
acute inflammation and tissue mast cells in adrenalectomized rats with cutaneous mucormycosis.	the effects of the absence of adrenal tissues on the inflammatory reaction and on the relationship of the tissue mast cells to the inflammatory process have been studied histologically in rats with cutaneous rhizopus oryzae infection. degranulation and regranulation of the tissue mast cells at the site of injury have been found to correlate respectively with the onset and subsidence of the exudative cellular phase of inflammation. in the adrenalectomized animals regranulation of these cells and ...	1963	14074382
on a new flavin mononucleotide produced by rhizopus oryzae.		1963	14109857
the germination of sporangiospores of rhizopus arrhizus; spore swelling and germ-tube emergence.		1964	14184103
[a case of mucormycosis (rhizopus oryzae)].		1964	14196841
studies on carbohydrate-metabolizing enzymes. 11. the hydrolysis of lichenin by enzyme preparations from malted barley and rhizopus arrhizus.		1964	5833367
role of proline and glutamic acid metabolism in spore germination of rhizopus arrhizus.		1966	5901876
further studies on germination of sporangiospores of rhizopus arrhizus.		1966	5912591
[lipase of rhizopus aerhizus. obtaining, purification and properties of the lipase of rhizopus arrhizus var. delemar].		1966	5958554
a kinetic method for the determination of number-average molecular weight of linear high polymer by using an exo-enzyme. number-average molecular weights of various fractions of amylose determined by using gluc-amylase of rhizopus delemar.		1966	5970083
antifungal activity of isothiocyanates and related compounds. i. naturally occurring isothiocyanates and their analogues.	data are presented concerning the antifungal activity of 11 natural isothiocyanates and 27 synthetized analogues in aspergillus niger, penicillium cyclopium, and rhizopus oryzae, as well as in 13 additional saprophytic and parasitic fungi. a remarkable antifungal activity was observed in some analogues of benzylisothiocyanate and beta-phenylethylisothiocyanate. the latter-mentioned compounds have not been described previously. in the group of benzylisothiocyanates, a correlation, which was inver ...	1967	6049294
antifungal activity of isothiocyanates and related compounds. ii. mononuclear aromatic isothiocyanates.	antifungal activity on aspergillus niger, penicillium cyclopium, and rhizopus oryzae, as well as on additional saprophytic and parasitic fungi, was determined in 57 substituted derivatives of phenylisothiocyanate. most of the investigated compounds displayed rather equal activity against the three mentioned fungi, in contradistinction to the analogues of natural benzyl- and beta-phenylethylisothiocyanate with their characteristic low activity against r. oryzae. differences occurred in the type o ...	1967	6049295
[on rhizopus arrhizus lipase. i. positional specificity].		1967	6055266
purification of a lipase from rhizopus arrhizus. identification of two active forms of the enzyme.		1967	6078132
[lipase of rhizopus arrhizus. isolation and properties of the pure enzyme].		1968	5716418
[rhizopus arrhizus lipase. i. action of the lipase on the triglycerides of chylomicrons and lipoproteins in vitro].		1968	4236043
[rhizopus arrhizus lipase. ii. action of lipase on chylomicrons in vivo].		1968	4236044
ultrastructural details in germinating sporangiospores of rhizopus stolonifer and rhizopus arrhizus.	electron microscope examination of sporangiospore sections from rhizopus stolonifer (ehrenb. ex fr.) lind. and r. arrhizus fischer revealed details on intracellular organization not previously reported. aldehyde fixation followed by chromeosmium postfixation permitted clear depiction of ribosomes hitherto unrevealed in these cells. mitochondria were diversiform. spore wall structures in the two species were generally similar, but outer contours differed sufficiently to permit easy species identi ...	1968	4876136
stereochemistry of microbiological hydroxylation. 3. hydroxylation of 3-benzoyl-3-azabicyclo[3.3.1]nonane with rhizopus arrhizus. novel chromic acid oxidations of substrate and product.		1969	5357524
some properties of a lipase from rhizopus arrhizus. separation of a glycopeptide bound to the enzyme.		1969	5363985
characterization of ribosomes from the phycomycetes, rhizopus arrhizus and pythium debaryanum.		1970	5413474
hemolytic activity of rhizopus nigricans and rhizopus arrhizus.		1970	5466029
lipids of rhizopus arrhizus fischer.	the lipids of rhizopus arrhizus fischer mycelia and sporangiospores were extracted, isolated, and separated by thin-layer, liquid, and gas chromatography. structural confirmations of the compounds were made by a gas chromatographmass spectrometer combination. the n-heptane fraction contained squalene (1%) as a major hydrocarbon constituent. other major lipid classes detected were free fatty acids, naturally occurring methyl esters of fatty acids, triglycerides, sterols, and polar lipids. the pol ...	1970	5474875
[some morphological and physiological properties of rhizopus delemar].		1970	5497282
pharmacological and toxicological studies on a starch diastatic enzyme "new sumyzyme" produced by rhizopus delemar var. multiplici-sporus.		1970	5535754
[i. transformation of scillarenin by rhizopus arrhizus. ii. structure clarification of the scillarenin transformation products of rhizopus arrhizus].		1971	5154908
[purification and properties of the beta-(1-3)-glucanase of rhizopus arrhizus].		1971	5159957
the exocellular lipase from rhizopus arrhizus.		1971	4997866
an nad + -independent l-lactate dehydrogenase from rhizopus oryzae.		1971	4334855
fatty acid ethyl esters of rhizopus arrhizus.	gas chromatographic and mass spectrometric analyses on selected lipid fractions revealed for the first time the presence of ethyl esters of long-chain fatty acids as biological products. ethyl esters of oleic, palmitic, and stearic acids were detected in relative concentrations of 21.2, 2.4, and 1.5 percent, respectively, of the total methyl and ethyl ester fraction. both saturated and unsaturated ethyl esters contain pronounced mass spectral fragments at a mass-to-charge ratio of 88.	1971	17792945
freezing artifacts in frozen-etched rhizopus arrhizus sporangiospores.		1972	4343254
effect of dimethyl sulphoxide on growth, sporulation and spore germination of rhizopus arrhizus fischer.		1972	5024636
total, polar and neutral lipids of rhizopus arrhizus fischer.		1972	5038496
further studies on the exocellular lipase of rhizopus arrhizus.		1972	5046808
tryptophan stimulation of growth and sporulation of rhizopus arrhizus fischer.		1972	5052891
characteristics of an in vitro phenylalanine incorporating system from rhizopus arrhizus.		1972	5061184
changes in esters of fatty acids of rhizopus arrhizus during germination and growth.	natural esters of fatty acids of rhizopus arrhizus fischer increased during the first 48 hr of growth. during the 48- to 72-hr period, there was a shift in concentration from the methyl esters to the ethyl esters of fatty acids.	1972	5079078
total lipid and sterol components of rhizopus arrhizus: identification and metabolism.		1973	4705432
ultrastructure of dormant and germinated sporangiospores of rhizopus arrhizus.		1973	4707020
roles of tryptophan residues on the rhizopus delemar lipase activity: chemical modification in a water-olive oil emulsion.		1973	4778940
microbiological hydroxylation of steroids. ix. hydroxylation of diketones and keto-alcohols derived from 5 alpha-androstane with the fungi rhizopus arrhizus and rhizopus circinnans. steroidal 18- and 19-proton magnetic resonance signals.		1973	4796662
the suitability of lipase from rhizopus arrhizus delemar for analysis of fatty acid distribution in dihexosyl diglycerides, phospholipids and plant sulfolipids.		1973	4214753
studies on the structure and mechanism of action of glycoside hydrolases. i. purification and study of some factors affecting the activity of rhizopus arrhizus (1 leads to 3)-beta-d-glucanase.		1974	4842010
influence of oxygen substituents at the c-11, -17, -20, and -21 positions on the extent of 6beta-hydroxylation of steroids by the mold rhizopus arrhizus.		1974	4845230
some properties of an exocellular lipase from rhizopus arrhizus.		1974	4597007
degradation of dyfonate in soil inoculated with rhizopus arrhizus.		1974	4134574
treatment of exocrine pancreatic insufficiency with fungal lipase (author's transl).	7 patients suffering from severe exocrine pancreatic insufficiency have been treated with a lipolytic enzyme extracted from rhizopus arrhizus. comparing the fungal lipase with a placebo the drug lowered the daily stool weight from 809 g to 443 g on an average, i.e. by 45.2%. the steatorrhea was reduced from 75.6 g/24 h to 32.9 g, i.e. by 56.5%. when incubating the enzyme in vitro in saline solutions of different ph for 1 h the loss of lipolytic activity is 13% at ph 5, 15% at ph 4, and 19% at ph ...	1975	1053
environmental factors affecting the degradation of dyfonate by soil fungi.	the ability of selected fungi to degrade the soil insecticide dyfonate (o-ethyl s-phenyl ethylphosphonodithioate) into water-soluble, noninsecticidal metabolites was found to be dependent on the supply of nutrients, incubation time, temperature, ph, as well as other factors. with yeast extract as the carbon source (5 g/liter) and ammonium nitrate (1 g/liter) as the nitrogen source, both rhizopus arrhizus and penicillium notatum degraded the insecticide to a larger extent than with any other comb ...	1975	46772
mucormycosis in a transplant recipient.	mucormycosis classically occurs in patients who have uncontrolled diabetes who develop rhinocerebral disease. a fatal case of rhinocerebral infection caused by rhizopus arrhizus in a 53-year-old man who had received a renal homograft three years previously is reported. only five similar cases have been documented, all since 1970. although direct smears of the purulent nasal exudate revealed the presence of numerous gram-negative bacilli, later identified as haemophilus influenzae, the diagnosis ...	1975	1099897
fungal air spora at ibadan, nigeria.	the fungal air spora at ibadan, nigeria, was investigated by using casella slit samplers. three sites, incorporating three locations at each site, were selected for the exposure of replicate plates during sampling. to provide data on a wide range of saprophytic and pathogenic fungal spores, isolations were made on sabouraud dextrose agar and malt agar plates incubated at 26 and 37 c. altogether over 60,000 fungal colonies were isolated and counted during the 12-month sampling period. the prevale ...	1975	1124919
effects of local anaesthetics on the lipase of rhizopus arrhizus.	1. a number of local anaesthetics were shown to inhibit the hydrolytic activity of a partially purified lipase from the mold rhizopus arrhizur towards both triacylglycerol and phospholipid substrates. 2. irrespective of whether triacylglycerol or phospholipid substrates were used, the inhibition was uncompetitive with respect to the substrate, independent of ca2+ concentration, but ph dependent. 3. the inhibitory activity of the local anaesthetics studied closely paralleled the anaesthetic poten ...	1976	1252490
the relative susceptibility of new zealand black and cba mice to infection with opportunistic fungal pathogens.	the susceptibility of ageing new zealand black (nzb) mice to infection with a number of opportunistic fungi and yeasts wsd compared with that of similarly aged mice of the cba strain. the nzb mice showed a greater susceptibility to lethal infection with aspergillus fumigatus, candida albicans and cryptococcus neoformans than did those of the cba strain. both mouse strains showed similar susceptibility to lethal infection by allescheria boydii, absidia corymbifera, mucor pusillus and rhizopus ory ...	1976	772839
fungal infections after renal transplantation.	fungal infections occurred in 3 of 22 renal transplants (13.6%). two patients developed rhinocerebral phycomycosis, and rhizopus arrhizus was isolated from cultures of the nasal conchae. both patients were treated with systemic amphotericin b. three-and-a-half months after transplantation the third patient developed an acute ureteral obstruction secondary to infection with aspergillus nidulans; this necessitated removal of the graft. there was no immediate mortality associated with these fungal ...	1976	786944
antimicrobial activity of econazole and miconazole in vitro and in experimental candidiasis and aspergillosis.	the antibacterial and antimycotic activity of econazole base, an imidazole derivative, was examined in vitro and in experimental infections of mice. comparative minimal inhibitory concentration (mic) determinations indicate econazole as well as miconazole to be of moderate activity against gram-positive bacteria (mics: 0.78-25mug/ml) and yeasts (mics: 1.56-25 mug/ml). against filamentous fungi, econazole exhibits better in vitro activity than miconazole and - with the exception of rhizopus oryza ...	1976	817875
simplified, totally enzymatic method for determination of serum triglycerides with a centrifugal analyzer.	we describe a totally enzymatic method for determination of serum triglycerides (triacylglycerols) specifically adaptable to the centrifichem system. the method involves lipolysis with lipase from rhizopus arrhizus alone and quantitation of the resulting glycerol with glycerol dehydrogenase in a kinetic, fixed-time mode. hydrolysis by the lipase is complete, for concentrations up to at least 5.0 g/liter, in 10 min at room temperature. the unfavorable equilibrium for the oxidation of glycerol is ...	1976	7367
phycomycotic gangrenous cellulitis. a report of two cases and a review of the literature.	progressive gangrenous cellulitis due to rhizopus arrhizus following colostomy destroyed the entire abdominal wall of a young woman and caused her death. a similar infection in an 11-year-old kidney transplant recipient was diagnosed more promptly and treated successfully with extensive debridement and amphotericin b. nine similar cases found in the literature were reviewed. all 11 patients appeared to have had prior tissue injury at the original site of infection, and seven had diabetes mellitu ...	1976	131532
study of bound phospholipase activities of fungal mycelia using an organic solvent system.	it has been possible to demonstrate and characterize high phospholipase activities in mycelia of rhizopus arrhizus and mucor javanicus by use of a system in which substrates were dissolved in diisopropyl ether. such activities were associated with bound enzymes and would have been difficult to detect using aqueous assay systems. in both cases, phosphatidylcholine hydrolysis was by phospholipase a1 (ec 3.1.1.32) activity followed by the action of lysophospholipase (ec 3.1.1.5). phospholipase d (e ...	1976	948251
counterimmunoelectrophoresis and opportunistic fungal infections.	sera from 35 apparently normal humans, 37 compromised human patients, 30 hedgehogs and 30 sheep, were examined for precipitating antibodies to four opportunistic fungi - absidia corymbifera, aspergillus fumigatus, candida albicans and rhizopus arrhizus-using counterimmunoelectrophoresis (cie). precipitins to a. fumigatus were almost exclusively confined to specimens obtained from the compromised human group (51% of those examined) while candida precipitating antibodies were detected in the sera ...	1977	321971
effects of amidination and chemical cross-linking on human factor viii (antihemophilic factor).	the bifunctional reagent dimethyl suberimidate, reacting with primary amino groups of proteins, was used to cross-link highly purified human factor viii. reaction products were reduced with beta-mercaptoethanol or treated with rhizopus arrhizus triglyceride lipase. the proportions of the dissociated subunits and their oligomers were calculated from the relative staining intensities of individual bands following polyacrylamide electrophoresis in the presence of sodium dodecyl sulfate. low concent ...	1977	410451
pancreatic and microbial lipases: a comparison of the interaction of pancreatic colipase with lipases of various origins.	conjugated bile salts inhibit the the hydrolysis of triglycerides (tg) by the lipases from rhizopus arrhizus and geotrichum candidum. this occurs for detergent concentrations similar to those which suppress the action of mammalian pancreatic lipases upon the same substrates. however, in opposition with what is observed with the latter enzymes, the activity is not restored by the addition of pancreatic colipase. both pancreatic and r. arrhizus lipases are inactivated at tributyrin/water interface ...	1977	558492
glyceride synthesis by four kinds of microbial lipase.	apart from their usual mechanism of action, lipases from aspergillus niger and rhizopus delemar also catalyzed the synthesis of glycerides from oleic acid and glycerol. lipases from geotrichum candidum and penicillium cyclopium were inactivated by oleic acid, but were stable in the presence of casein, albumin or buffer of appropriate ph. lipases from aspergillus niger and rhizopus delemar synthesized glycerides from, not only fatty acid, but dibasic acids and aromatic acids, making ester bonds o ...	1977	563245
synchronous sporulation during idiophase development in surface cultures of rhizopus arrhizus (fischer).	a method to obtain synchronous sporulation during idiophase development in liquid surface cultures of rhizopus arrhizus fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. in this system growth and sporulation are effectively separated. the respiratory pattern indicates three metabolic phases. the first is associated with spore germination; the second, trophophase, with growth; and the third, idiophase, with sporulation. synchronous sporulation ...	1977	890600
lipase hydrolysis of mammalian long-chain 1,2-alkanediol diesters. nonrandom distribution of fatty acids.	long-chain 1,2-alkanediol diesters were isolated from the total surface lipids of golden syrian hamsters and swiss albino mice. hydrolysis of the diol diester waxes with exocellular lipase from rhizopus arrhizus delemar or with purified porcine pancreatic lipase produced free fatty acids and 2-acyl diols in about 60--80% yield. nonrandom distribution of the constituent fatty acids at positions 1 and 2 of the alkanediols was observed. in the diester waxes from the hamster, both straight-chain and ...	1978	568644
purification, characterization, and action-pattern studies on the endo-(1 linked to 3)-beta-d-glucanase from rhizopus arrhizus qm 1032.	the extracellular (1 linked to 3)-beta-d-glucanase [(1 linked to 3)-beta-d-glucan glucanohydrolase, ec 3.2.1.6] produced by rhizopus arrhizus qu 1032 was purified 305-fold in 70% overall yield. this preparation was found to be homogeneous by ultracentrifugation (sedimentation velocity and equilibrium studies), electrophoresis on acrylamide gel with normal, sodium dodecyl sulfate, and urea-acetic acid gels, and upon isoelectric focusing. the amino acid composition of the enzyme has been determine ...	1978	647706
[experiences with substitution therapy using a new pancreatic enzyme of plant origin].	the indication field of nortase, a combination of microbial lipolytic and proteolytic enzymes, comprises the replacement therapy of maldigestion and insufficiency of pancreas. its efficacy and tolerance were tested in 100 patients in an open study under the conditions of general practice. during the 15-day treatment the following symptoms were evaluated: anorexia, flatulence, pressure and pain in the epigastrium, nausea after the meals, belching, pyrosis, the quality of feces and the body weight ...	1978	700583
synthesis of trinitrophenylaminolauric acid and the use of its glyceryl esters for assaying lipase by a spectrophotometric procedure.	trinitrophenylaminolauric acid was synthesized from omega-aminolauric acid and trinitrobenzenesulfonic acid and then condensed with glycerol to yield mono-, di- and triacylglyceryl esters of this acid. hydrolysis of these glycerides was followed by isolation of the yellow fatty acid with the aid of one solvent extraction step and estimating its content by spectrophotometry. this procedure was used to assay the activities of lipases from hog pancreas, rat bile, microsomes of rat brain and rhizopu ...	1978	718973
damage to hyphal forms of fungi by human leukocytes in vitro. a possible host defense mechanism in aspergillosis and mucormycosis.	evidence suggests that neutrophils are important in host defenses against invasive aspergillosis and mucormycosis, although hyphae in these lesions are too large to be phagocytized. interactions of neutrophils with hyphae of aspergillus fumigatus and rhizopus oryzae were studed in vitro. light and electron microscopic observations indicated that neutrophils attached to and spread over the surfaces of hyphae, even in the absence of serum. this was followed by dramatic morphologic changes which su ...	1978	347942
the use of fluorescein-labelled lectins in the detection and identification of fungi pathogenic for man: a preliminary study.	fluorescein-labelled lectins of known specificities for different sugars were used in an attempt to identify fungi in paraffin sections of surgical and post-mortem material. aspergillus fumigatus, blastomyces dermatitidis, candida albicans, cryptococcus neoformans, paracoccidioides brasiliensis and rhizopus oryzae have been studied with five fluorescein-labelled lectins and with basis of differences in their reactions with these stains. the results accord well with what is known of the chemistry ...	1978	355633
development and evaluation of an immunodiffusion test for diagnosis of systemic zygomycosis (mucormycosis): preliminary report.	an antigen analysis with filtrate and homogenate precipitinogens of single isolates of the zygomycetes absidia corymbifera, mucor pusillus, rhizopus arrhizus, and rhizopus oryzae demonstrated the presence of common antigens among the three genera as well as antigens which permit their differentiation. selected homogenate antigens were valuable in developing a diagnostic immunodiffusion (id) test for systemic zygomycosis. when sera from 43 patients with various proven mycoses other than zygomycos ...	1978	75212
the post-harvest fruit rots of tomato (lycopersicum esculentum) in nigeria.	a survey of the post-harvest fruit rot diseases of tomato was conducted in five states of nigeria. during severe infections, the diseases could cause 25% loss at harvest and 34% loss of the remaining product in transit, storage and market stalls; thus giving an overall loss of about 50% of the product. two types of rots, soft and dry were recognised. the soft rot was found to account for about 85% and the dry rot about 15% of the overall loss. erwinia carotovora, rhizopus oryzae, r. stolonifer, ...	1979	471028
orbitofacial mucormycosis with unusual pathological features.	a 52-year-old man with mild diabetes and acute stem cell leukaemia developed an orbitofacial mucormycosis. cultures showed the fungus to be rhizopus oryzae. vigorous treatment with amphotericin b and other bactericidal and bacteriostatic antibiotics for a concurrent sepsis failed to suppress the infections, and the patient died. on post-mortem examination characteristic haematoxylin-staining, broad, aseptate fungal hyphae were found in the right eye, orbit, and lung. a striking and unusual featu ...	1979	508683
1,3-beta-d-glucanases from pisum sativum seedlings. ii. substrate specificities and enzymic action patterns.	two purified pea 1,3-beta-d-glucanases (ec 3.2.1.6) hydrolyse laminarin (degree of polymerization 20), laminaridextrins (degree of polymerization 3--7), and their reduced 3h-derivatives, 1,3-beta-d-glucans which are partially substituted (carboxymethyl-pachyman) or crystalline (curdlan), and mixed-linkage beta-glucans. enzyme kinetics and product-formation indicate endo-hydrolase activity with weak transglycosylase capacity. the enzymes do not hydrolyse beta-glucosides, the 1,3 linkage adjacent ...	1979	508768
synthesis of various kinds of esters by four microbial lipases.	ester synthesis by microbial lipases, using homogeneous enzyme preparations, were investigated. the amount of synthesized ester was estimated by alkalimetry, and products were identified by thin-layer chromatography and infrared spectroscopy. lipases from aspergillus niger, rhizopus delemar, geotrichum candidum and penicillium cyclopium synthesized esters from oleic acid and various primary alcohols. only geotrichum candidum lipase synthesized esters of secondary alcohols. esters of tertiary alc ...	1979	508776
[further examinations of the influence of somatostatin on triglyceride absorption (author's transl)].	7 healthy subjects received an oral fat load containing 100 g neutral fat on two test days. the test meal was followed by an infusion of somatostatin (500 micrograms/h) over three hours. on the first test day 150,000 e of an active fungal lipase (extracted from rhizopus arrhizus) were given with the fat meal. on the second day placebo capsules were administered instead of lipase. no postprandial increase of serum triglyceride levels could be found in both examinations. these results suggest, tha ...	1980	6104384
nosocomical rhizopus infection (zygomycosis) in children.	three children with the rare occurrence of zygomycosis are descibed: two had involvement of a solitary lesion of gangrenous cellulitis on the buttocks, and th third was a neonate with gastric performation and a gangrenous appendicitis. all three patients were compromised hosts (two with leukemia and one a premature infant with respiratory distress syndrome). all three patients appeared to have acquired the same organism. rhizopus oryzae, from the same fomites, elastic bondages (elastoplast). the ...	1980	6928943
some studies on the composition and surface properties of oil bodies from the seed cotyledons of safflower (carthamus tinctorius) and linseed (linum ustatissimum).	1. the average oil-body diameter in intact cells of developing linseed (linum usitatissimum) and safflower (carthamus tinctorius) cotyledons was similar (about 1.4 micrometer), and there was little change in size after oil bodies were isolated and repeatedly washed. 2. the glycerolipid composition of washed oil bodies from both developing and mature cotyledons of the two species was similar; oil bodies from ten different batches of cotyledons contained 4.3 +/- 0.16 mumol of 3-sn-phosphatidylchol ...	1980	7008782
susceptibility of zoopathogenic fungi to phytoalexins.	phytoalexins are a group of low-molecular-weight antibiotics produced by higher plants in response to infection by relatively avirulent microorganisms. they are of relatively low toxicity for mammalian cells and have been reported to possess a broad spectrum of antimicrobial activity against bacteria and phytopathogenic fungi. employing a broth dilution technique, we have found the zoopathogens petriellidium boydii, aspergillus flavus, a. fumigatus, candida albicans, coccidioides immitis, crypto ...	1980	7387136
microbiological transformation of 1,2-diphenyl-3,5 dioxo-4-n-butyl pyrazolidine (phenylbutazone).	microbiological conversion of phenylbutazone with rhizopus arrhizus fischer gave 4-hydroxy phenylbutazone in excellent yield. spectral studies, mass spectrometry, in particular, were made of the obtained metabolite; other experiments performed with the intact microorganism or with the cell-free system, as well as studies with the well-known metabolic inhibitor, skf 525 a, indicated that the obtained 4-hydroxy phenylbutazone was not an artefact. similar experiments were performed on oxyphenbutazo ...	1980	7389746
fungi from interior organs of free-living small mammals in czechoslovakia and yugoslavia.	a total of 308 fungi was isolated from interior organs (lungs, spleen, liver) of 529 small mammals belonging to 21 species, 7 families and 3 orders (insectivora, chiroptera, rodentia), some of these being potentially pathogenic to vertebrates (e.g. aspergillus flavus, a. fumigatus, geotrichum candidum, mucor pusillus, rhizopus arrhizus). in one vole (microtus arvalis) captured in south moravia, adiaspiromycosis (emmonsia crescens) was demonstrated. comparison of mycoflora of hair and that of int ...	1980	7419129
modification of rhizopus delemar lipase by its binding with phospholipids.	rhizopus delemar (atcc 34612) lipase was modified by phospholipid (pl)-treatment so as to enhance its activity on lipoprotein. in order to detect change in lipase conformation in the modified state, a preliminary experiment was performed to remove pl from the pl-treated lipase solution which included pl nonessential to enhancement of lipoprotein lipase (lpl) activity. it was found that treatment with a mixture of isopropyl ether: n-butanol (3 : 1) was suitable for this purpose because of the sta ...	1980	7419510
fatty acid elongation by a particulate fraction from germinating pea.	the synthesis of fatty acids from [14c]malonyl-coa was studied with a high-speed particulate fraction from germinating pea (pisum sativum). the variety used (feltham first) produced mainly saturated fatty acids with palmitate (30--40%) and stearate (40--60%) predominating. several palmitate-containing lipids stimulated overall synthesis and, in addition, increased the percentage of label in stearate. the production of stearate was severely inhibited by preincubation of the microsomal fraction wi ...	1980	7283974
reversibility of the modification of rhizopus delemar lipases by phosphatidylcholine.	rhizopus (rh.) delemar (atcc 34612) lipase is modified by its binding with phosphatidylcholine (pc); such binding enhances the lipoprotein lipase (lpl) activity, shifts the isoelectric point (pi) to the acidic side and decreases its alpha-helical content ((1980) j. biochem. 88, 533-538). the results of density gradient ultracentrifugation proved that pc binding to lipase molecule was depleted by the treatment of pc-bound lipase with 0.3% triton x-100 and 0.1 m nacl. by this treatment, lpl activi ...	1981	7287646
preparation of thioester substrates and development of continuous spectrophotometric assays for phospholipase a1 and monoacylglycerol lipase.	current assays of phospholipase a1 (ec 3.1.1.32) and monoacylglycerol lipase (ec 3.1.1.23) activities in tissues are discontinuous, laborious, and expensive. some spectrophotometric substrates were synthesized to alleviate this problem. thioester analogs of phosphatidylcholine and phosphatidylethanolamine. rac-1,2-s,o-didecanoyl-3-phosphocholine-1-mercapto-2,3-propanediol and rac-1,2-s,o-didecanoyl-3-phosphoethanolamine-1-mercapto-2,3-propanediol, were synthesized from the diacylglycerol analog, ...	1981	7240974
[immobilized lipase from rhizopus oryzae 14-14].	properties of lipase immobilized from rhizopus oryzae 14-14 immobilized on inorganic supports-aluminium oxide and silica gel-were investigated. aluminium oxide immobilized lipase retained 23% of its initial activity when tributyrin was used as substrate. ph optimum of action of immobilized lipase was at 8.75, i.e. it was shifted toward the more alkaline area as compared to ph optimum of the non-immobilized enzyme which was in the area of 7.0-7.8. the temperature optimum of action of the immobili ...	1981	7279881
is platelet-activating factor (paf-acether) synthesis by murine peritoneal cells (pc) a two-step process?	paf-acether (1-o-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) is released from several cell sources simultaneously with an inactive non-acylated compound (1-o-alkyl-glyceryl-3-phosphorylcholine) (lyso-paf-acether). formation of the latter probably results from the activation of a phospholipase a2 (pla2). indeed, the pla2 inhibitors, bromophenacyl bromide (bpb), mepacrine, 874cb (100 microm), and edta (5 mm), blocked the zymosan-induced release of paf-acether from pc. edta and bpb also markedl ...	1981	7053072
inducibility of cytochrome p-450 and of nadph-cytochrome c reductase in progesterone treated filamentous fungi rhizopus nigricans and rhizopus arrhizus.		1981	6785529
a mechanism of susceptibility to mucormycosis in diabetic ketoacidosis: transferrin and iron availability.	the defect in host defense that makes the diabetic ketoacidotic (dka) patient susceptible to mucormycosis has not been identified. sera from 10 dka patients and three normal volunteers were tested for their capacity to support the in vitro growth of a common etiologic agent of mucormycosis, rhizopus oryzae. after equilibration with room air none of the normal or dka sera, each of which was now extremely alkaline, supported growth of r. oryzae. when the sera were placed in a co2 atmosphere that p ...	1982	6816646