Publications

the mycoparasite ampelomyces quisqualis expresses exga encoding an exo-beta-1,3-glucanase in culture and during mycoparasitism.	abstract ampelomyces quisqualis, a mycoparasite of fungi causing powdery mildews, exhibited high levels of extracellular exo-beta-1,3-glucanase activity in culture compared with neurospora crassa and gliocladium roseum. a. quisqualis culture filtrates affected powdery mildew caused by sphaerotheca fusca in a manner indicative of cell wall degradation, as determined by microscopic examination. a gene encoding an exo-beta-1,3-glucanase in a. quisqualis, designated exga, was isolated and sequenced. ...	1999	18944674
occurrence and molecular characterization of strobilurin resistance in cucumber powdery mildew and downy mildew.	abstract between 1998 and 1999, control failure of powdery mildew (podosphaera fusca) and downy mildew (pseudoperonospora cubensis) by the strobilurin fungicides azoxystrobin and kresoxim-methyl was observed in cucumber-growing areas of japan. results from inoculation tests carried out on intact cucumber plants and leaf disks clearly showed the distribution of pathogen isolates highly resistant to azoxystrobin and kresoximmethyl. fragments of the fungicide-targeted mitochondrial cytochrome b gen ...	2001	18943331
effect of mycoparasitic fungi on the development of sphaerotheca fusca in melon leaves.	we evaluated the possibilities of exploitation of several mycoparasitic fungi in the biocontrol of cucurbit powdery mildew (sphaerotheca fusca) in melon greenhouses. to simplify the screening, an in vitro biological control test on detached leaves of melon has been used and a detailed microscopic analysis of the interactions between mycoparasites and s. fusca conducted. in this context, the effect of mycoparasitic fungi on the formation of infection structures of s. fusca such as haustoria, coni ...	2003	12735245
changes in specific activities of peroxidase, chitinase and phenylalanine ammonia-lyase and phenolic content in cucumber leaves inoculated with podosphaera fusca, the causal agent of powdery mildew.	in this investigation, the effects of powdery mildew disease [caused by podosphaera fusca (syn. sphaerotheca fuliginea)] on specific activities of several defense-related enzymes and phenolic content were studied in cucumber leaves. spore suspension of the fungus was sprayed on cucumber (cv. super dominus) plants in greenhouse and leaves from both inoculated and non-inoculated control plants were sampled at 0, 24, 48, 72 and 144 hours after inoculation (hai). spore germination and tissue coloniz ...	2004	15756838
isolation and evaluation of antagonistic bacteria towards the cucurbit powdery mildew fungus podosphaera fusca.	powdery mildew is one of the most important limiting factors for cucurbits production in spain, its management being strongly dependent on chemicals. the aim of this work was to evaluate the possibility of exploiting antagonistic bacteria in the biological control of the cucurbit powdery mildew fungus podosphaera fusca (syn. sphaerotheca fusca). among a collection of bacterial strains isolated from distinct cucurbit powdery mildew diseased plants and rhizospheric soils, four isolates were select ...	2004	13680203
effect of lipopeptides of antagonistic strains of bacillus subtilis on the morphology and ultrastructure of the cucurbit fungal pathogen podosphaera fusca.	to analyse the morphological and ultrastructural effects of lipopeptides of cell-free liquid cultures from the antagonistic bacillus subtilis strains, umaf6614 and umaf6639, on the cucurbit powdery mildew fungus, podosphaera fusca, conidial germination.	2007	17897200
the iturin and fengycin families of lipopeptides are key factors in antagonism of bacillus subtilis toward podosphaera fusca.	podosphaera fusca is the main causal agent of cucurbit powdery mildew in spain. four bacillus subtilis strains, umaf6614, umaf6619, umaf6639, and umaf8561, with proven ability to suppress the disease on melon in detached leaf and seedling assays, were subjected to further analyses to elucidate the mode of action involved in their biocontrol performance. cell-free supernatants showed antifungal activities very close to those previously reported for vegetative cells. identification of three lipope ...	2007	17427813
multiple displacement amplification, a powerful tool for molecular genetic analysis of powdery mildew fungi.	powdery mildew fungi (erysiphales) are probably the largest group of plant pathogens that remain uncharacterized from genetic and molecular points of view, with the only exception of the powdery mildew of cereals, blumeria graminis. their nature as obligate biotrophic parasites and consequent inability to grow on culture media has significantly hampered research. a common bottleneck to the molecular genetic analysis of powdery mildew fungi is the availability of genomic dna of suitable quality a ...	2007	17256172
field resistance to qoi fungicides in podosphaera fusca is not supported by typical mutations in the mitochondrial cytochrome b gene.	a single nucleotide polymorphism in the mitochondrial cytochrome b gene confers resistance to strobilurin (qoi) fungicides in phytopathogenic fungi. recent studies have revealed worrying levels of resistance to strobilurins in podosphaera fusca (fr.) u braun & n shishkoff comb. nov. [ = sphaerothecafusca (fr.) s blumer], the main causal agent of cucurbit powdery mildew in spain. in the present study the underlying resistance mechanism to qoi fungicides in the spanish populations of p. fusca was ...	2008	18247319
comparative histochemical analyses of oxidative burst and cell wall reinforcement in compatible and incompatible melon-powdery mildew (podosphaera fusca) interactions.	the spatial-temporal expression patterns of oxidative burst and cell wall reinforcement were analyzed in leaves of resistant and susceptible melon (cucumis melo l.) cultivars in response to podosphaera fusca (fr.) braun & shishkoff, the main causal agent of powdery mildew in cucurbits. extensive development of powdery mildew mycelia and a progressive increase in haustorial count were recorded in the susceptible cultivar after 4d, while in the resistant cultivar powdery mildew failed to grow and ...	2008	18585824
survey of microfungi in the kleinwalsertal (austrian alps).	during an excursion to the alps near the german/austrian border (kleinwalsertal) in august 2007, we were able to collect more than 40 species of microfungi as parasites or saprophytes on different parts of wild plants. some of them have been observed only rarely until now. most of the species collected belong to the classes ascomycotina, basidiomycotina, and deuteromycotina. for example: -leptosphaeria jaceae holm on centaurea jacea l.; -mycosphaerella equiseticola bond.-mont. on equisetum telma ...	2008	19226751
the powdery mildew fungus podosphaera fusca (synonym podosphaera xanthii), a constant threat to cucurbits.	numerous vegetable crops are susceptible to powdery mildew, but cucurbits are arguably the group most severely affected. podosphaera fusca (synonym podosphaera xanthii) is the main causal agent of cucurbit powdery mildew and one of the most important limiting factors for cucurbit production worldwide. although great efforts have been invested in disease control, by contrast, many basic aspects of the biology of p. fusca remain unknown. taxonomy: podosphaera fusca (fr.) braun & shishkoff. kingdom ...	2009	19236565
sensitivities to dmi fungicides in populations of podosphaera fusca in south central spain.	cucurbit powdery mildew elicited by podosphaera fusca (fr.) u braun & n shishkoff limits crop production in spain. disease control is largely dependent on fungicides such as sterol demethylation inhibitors (dmis). fungicide resistance is an increasing problem in this pathogen. to overcome such risk, it is necessary to design rational control programmes based upon knowledge of field resistance. the aim of this study was to investigate the state of dmi sensitivity of spanish p. fusca populations a ...	2010	20533378